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1985 Spring No. 7, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 7 Spring 1985 was edited by Russell Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 24 pages.

Interleukin 2 Regulates Its Own Receptors., Kendall A. Smith, Doreen A. Cantrell

Dartmouth Scholarship

The cell surface density of high-affinity membrane receptors for the T-lymphocytotrophic hormone interleukin 2 (IL-2) determines the rate of T-cell-cycle progression. Since 10-fold greater numbers of IL-2 receptor molecules were found by using a radiolabeled monoclonal antibody reactive with IL-2 receptors (anti-Tac) compared with binding of IL-2, the functional relationship of the binding sites recognized by both of these ligands was assessed. In the presence of cycloheximide, IL-2 binding sites declined with a half-time (t1/2) of 2.6 hr, whereas the decay of anti-Tac binding sites was much slower (t 1/2 = 6.4 hr). Moreover, after limited membrane proteolysis, the half-time …

Two Separable Functional Domains Of Simian Virus 40 Large T Antigen: Carboxyl-Terminal Region Of Simian Virus 40 Large T Antigen Is Required For Efficient Capsid Protein Synthesis., Joanne Tornow, Maryellen Polvino-Bodnar, George Santangelo, Charles N. Cole

Dartmouth Scholarship

The carboxyl-terminal portion of simian virus 40 large T antigen is essential for productive infection of CV-1 and CV-1p green monkey kidney cells. Mutant dlA2459, lacking 14 base pairs at 0.193 map units, was positive for viral DNA replication, but unable to form plaques in CV-1p cells (J. Tornow and C.N. Cole, J. Virol. 47:487-494, 1983). In this report, the defect of dlA2459 is further defined. Simian virus 40 late mRNAs were transcribed, polyadenylated, spliced, and transported in dlA2459-infected cells, but the level of capsid proteins produced in infected CV-1 green monkey kidney cells was extremely low. dlA2459 large T …

Faculty Publications 1984, Lsu Health Sciences Center New Orleans

Historical Faculty Publications

This Faculty bibliography contains 565 publications.

Clinical Findings In Four Children With Biotinidase Deficiency Detected Through A Statewide Neonatal Screening Program, Barry Wolf, Gregory S. Heard, Linda G. Jefferson, Virginia K. Proud, Walter E. Nance, Karen A. Weissbecker

Human and Molecular Genetics Publications

Four children with biotinidase deficiency were identified during the first year of a neonatal screening program for this disease in the Commonwealth of Virginia. Two unrelated probands were identified among the 81,243 newborn infants who were screened. In addition, two siblings of one of these infants were found to be affected. Both probands had mild neurologic symptoms at two and four months, respectively, and the two older children had more severe neurologic abnormalities, cutaneous findings, and developmental delay at two and three years of age. However, none of the affected children had acute metabolic decompensation. Previous studies have shown that …

Genetics, Eugenics, And Public Policy, George P. Smith Ii

Scholarly Articles

No abstract provided.

Anterior Communicating Artery Aneurysm And Vasospasm – A Computer Model [Poster Boards], G. Austin, R. Gaskell

The Dr. George M. Austin Neurology Research Collection

Poster presentation boards for “Anterior Communicating Artery Aneurysm and Vasospasm – A Computer Model,” G. Austin and R. Gaskell – Storke [sic] Research Foundation, Santa Barbara, Ca. and Dept. of Neurosurgery, Univ. of S. Cal., L.A., 5 p.

An Experimental Study Of The Longest Tolerable Time Of The Total Portal Triad Occlusion In Normothermia In Rabbit, X P Chen, Z D Wu

Faculty, Staff and Student Publications

No abstract provided.

Regulation Of Luteinizing Hormone And Catecholamine Release, Benjamin Aaron Adler

Theses and Dissertations (ETD)

These studies tested the interrelated hypotheses that the ovarian hormones produce their positive feedback effects on luteinizing hormone (LH) secretion through activation of noradrenergic and adrenergic systems in specific hypothalamic regions. Furthermore, the ovarian hormones may alter the activity of opioid neuropeptide and Gamma-Aminobutyric Acid (GABA) systems to produce these alterations in catecholamine transmission and gonadotropin secretion. Radioimmunoassays were utilized to determine plasma LH and median eminence LHRH, and hypothalamic catecholamine concentrations were measured by radioenzymatic assay.

The first two studies tested whether epinephrine (EPI) synthesis inhibition blocks the accumulation of median eminence LHRH that precedes the ovarian hormone-induced LH …

Genetics And Epidemiology Of Gallbladder Disease In New World Native Peoples, K M Weiss, R E Ferrell, C L Hanis, P N Styne

Faculty, Staff and Student Publications

Native peoples of the New World, including Amerindians and admixed Latin Americans such as Mexican-Americans, are highly susceptible to diseases of the gallbladder. These include cholesterol cholelithiasis (gallstones) and its complications, as well as cancer of the gallbladder. Although there is clearly some necessary dietary or other environmental risk factor involved, the pattern of disease prevalence is geographically associated with the distribution of genes of aboriginal Amerindian origin, and levels of risk generally correspond to the degree of Amerindian admixture. This pattern differs from that generally associated with Westernization, which suggests a gene-environment interaction, and that within an admixed population …

Detection Of Nonrandom Association Of Alleles From The Distribution Of The Number Of Heterozygous Loci In A Sample, R Chakraborty

Faculty, Staff and Student Publications

The distribution of the number of heterozygous loci in two randomly chosen gametes or in a random diploid zygote provides information regarding the nonrandom association of alleles among different genetic loci. Two alternative statistics may be employed for detection of nonrandom association of genes of different loci when observations are made on these distributions: observed variance of the number of heterozygous loci (s2k) and a goodness-of-fit criterion (X2) to contrast the observed distribution with that expected under the hypothesis of random association of genes. It is shown, by simulation, that s2k is statistically more efficient than X2 to detect a …

1984 Fall No. 6, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 6 Fall 1984 was edited by Richard M. Paddison, MD, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 24 pages and includes the special section "Reunion Scrapbook".

Extrathyroidal Human Biochemistry Of Monoiodotyrosine, Stanley Andrew Tan

Loma Linda University Electronic Theses, Dissertations & Projects

The existence of extrathyroidal monoiodotyrosine (MIT) has been debated because of uncertain results from measuring serum MIT. The reason is that the isotopic and chromatographic assays used have not been sensitive enough. This investigation undertook to establish the existence of MIT in serum utilizing a radioimmunoassay (RIA), to study the physiology of serum MIT, and to discover the pharmacologic effects of exogenous MIT.

MIT-binding antibody was raised in rabbits by immunization with an MIT-porcine thyroglobulin conjugate. The antiserum bound 40% of the labeled MIT at a final dilution of 1:1000. Incubation of 100 µ1 antiserum with 50 pg [^{125 …}

Effect Of Menstrual Cycle On Hedonic Response To Gustatory Stimuli, Laurie Ann O'Toole

Dissertations and Theses

The present experiment was conducted to determine the relationship between the human menstrual cycle and gustatory hedonic response. Hedonic response was defined as the perceived pleasantness or unpleasantness of a taste stimulus. Gender differences in taste hedonic have been observed; these may be due to differences in endocrine systems. Animal taste preferences are altered when reproductive hormonal status is experimentally manipulated. Previous researchers report that human females' hedonic responses to sucrose vary systematically with phase of menstrual cycle.

Metabolism In Preimplantation Mouse Embryos, Harry M. Weitlauf, Gary L. Nieder

Neuroscience, Cell Biology & Physiology Faculty Publications

The ability of preimplantation mouse embryos to utilize glucose oxidatively is controlled, in part at least, at the level of glycolysis. Various experimental observations are reviewed that indicate the regulatory mechanism in delayed implanting blastocysts involves the classic negative allosteric feedback of high levels of ATP on phosphofructokinase while the situation in 2-cell embryos appears to be more complicated. That is, in addition to the usual negative effect of ATP and citrate on phosphofructokinase, there appears to be a modification of hexokinase that prevents phosphorylation of adequate amounts of glucose and results in low levels of fructose-6-phosphate at the 2-cell …

The Effect Of Dietary Alpha - Linolenic Acid On Apparent Risk Factors Of Cardiovascular Disease, Kelvin D. Lindbeck

Loma Linda University Electronic Theses, Dissertations & Projects

Eight healthy male lacto-ovo-vegetarian subjects averaging 28.0 ± 3.6 years of age (22 to 53 years) were fed a diet rich in α-linolenic acid (18:3 ω 3) for four weeks. Arterial blood pressure and body weight were recorded each week. Plasma 6-keto-prostaglandin F_1α (6-keto- PGF_1α ), thromboxane B₂ (TXB₂), total cholesterol, triglyceride, and total phospholipid fatty acid composition were analyzed at 0, 2 and 4 weeks.

Systolic blood pressure increased significantly between weeks 0 and 1 (106.5 ± 2.7 mmHg to 114.0 ± 3.7 mmHg; p < 0.003), but decreased to 107.3 t 2.6 mrHg (p < 0.02) at week 2. There was an insignificant increase in both systolic and diastolic blood pressures after four weeks. There was no significant change in either total cholesterol or triglyceride levels during the four weeks.

Plasma 6-keto-PGF_1α levels increased significantly from 60.13 ± …

Lifeline - City Of Faith Newsletter (May-June, 1984), Holy Spirit Research Center Oru Library

City of Faith

Lifeline vol 2, no 3 (May-June - 1984)

Lifeline is the newsletter for the employees and staff of the City of Faith Medical and Research Center. This edition includes the following articles:

• "Quick Care Meeting South Tulsans' Needs"
• "Research at the City of Faith: The Quest for a Breakthrough"
• "Diabetes Institute Tests 'Miracle' Drug"
• "Grants Provide Research in Number of Areas"
• "Alzheimer's Disease Research Fund Established"
• "Optical Clinic Focuses on Quality Eye Care"
• "The Case for City of Faith Marketing"
• "Miracles Expected in Informations Systems"
• "COF Communications Reaches Out and Touches Everyone"
• "Welcome New Employees and Staff"

Inhibition Of Growth Of Toxoplasma Gondii In Cultured Fibroblasts By Human Recombinant Gamma Interferon., E. R. Pfefferkorn, Paul M. Guyre

Dartmouth Scholarship

The growth of Toxoplasma gondii in cultured human fibroblasts was inhibited by recombinant human gamma interferon at concentrations of 8 to 16 U/ml. The interferon was titrated by observing a total inhibition of parasite plaque formation 7 days after infection. Inhibition of the growth of T. gondii in the early days after infection was measured by marked reductions in the incorporation of radioactive uracil, a precursor that can only be used by the parasites. This assay showed that when cells were pretreated with gamma interferon for 1 day and then infected, inhibition of T. gondii growth could be readily detected …

Digital Computer Model Of An Intracranial Aneurysm, G. Austin, R. Gaskell

The Dr. George M. Austin Neurology Research Collection

Digital Computer Model of an Intracranial Aneurysm. G. Austin, R. Gaskell. Poster presentation. American Association of Neurologic Surgeons, April 8-12, 1984.

File includes Abstract, 1 pg; Presentation, 2 pg; Presentation draft, 3 pgs.

1984 Spring No. 5, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 5 Spring 1984 was edited by Richard M. Paddison, MD, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 16 pages.

Triggering Of The T3-Ti Antigen-Receptor Complex Results In Clonal T-Cell Proliferation Through An Interleukin 2-Dependent Autocrine Pathway., Stefan C. Meuer, Rebecca E. Hussey, Doreen A. Cantrell, James C. Hodgdon, Stuart F. Schlossman, Kendall A. Smith, Ellis L. Reinherz

Dartmouth Scholarship

Human T-cell clones and anti-T-cell-receptor antibodies (clonotypic) directed at surface receptors for antigen (T3-Ti molecular complex) as well as anti-interleukin 2 (IL-2) and anti-IL-2-receptor antibodies were utilized to investigate the mechanism by which alloantigens or antigen plus self-major histocompatibility complex (MHC) (i.e., physiologic ligand) trigger specific clonal proliferation. Soluble or Sepharose-bound anti-Ti monoclonal antibodies, like physiologic ligand, enhanced proliferative responses to purified IL-2 by inducing a 6-fold increase in surface IL-2 receptor expression. In contrast, only Sepharose-bound anti-Ti or physiologic ligand triggered endogenous clonal IL-2 production and resulted in subsequent proliferation. The latter was blocked by antibodies directed at either …

Body Weight Changes Throughout The Menstrual Cycle And Their Effect Upon The Components Of Body Composition, Lovina M. Query

Dissertations and Theses

Research has shown that women experience fluctuations in body weight (BW) due to water retention and/or other processes at various times in the menstrual cycle. The purpose of this study is to measure women repeatedly throughout the course of one menstrual cycle and

(1) to confirm whether significant fluctuations in weight occur at various times and

(2) to determine whether there are significant differences in the components of body composition by hydrostatic weighing which are related to any changes in BW.

It was concluded that while significant fluctuations in BW occur at certain times in the menstrual cycle, the changes …

Interferon Gamma Blocks The Growth Of Toxoplasma Gondii In Human Fibroblasts By Inducing The Host Cells To Degrade Tryptophan., E. R. Pfefferkorn

Dartmouth Scholarship

Treatment of human fibroblasts with human recombinant gamma interferon blocked the growth of Toxoplasma gondii, an obligate intracellular protozoan parasite. Growth of the parasite was measured by a plaque assay 7 days after infection or by the incorporation of [3H]uracil 1 or 2 days after infection. The antitoxoplasma activity induced in the host cells by gamma interferon was strongly dependent upon the tryptophan concentration of the medium. Progressively higher minimal inhibitory concentrations of gamma interferon were observed as the tryptophan concentration in the culture medium was increased. Treatment with gamma interferon did not make the cells impermeable to tryptophan. The …

The Changes Of Cortical Blood Flow And Cortical Oxygen Tension In Focal Ischemia, K. Kaminoga, Y. Taguchi, G. Austin

The Dr. George M. Austin Neurology Research Collection

The Changes of Cortical Blood Flow and Cortical Oxygen Tension in Focal Ischemia. K. Kaminoga, M.D., Y. Taguchi, M.D., G. Austin. The Stroke Research Foundation and Department of Environmental Stress, UC-Santa Barbara, Ca. Presented at the Southern California Chapter American college of Surgeons, Santa Barbara, Calif. 28 January 1984. 2 pg.

Behavioral Interactions Of Opioid Agonists And Antagonists With Serotonergic Systems, David J. Mokler, Richard H. Rech, Randall L. Commissaris, Judith W. Henck

Biomedical Sciences Faculty Publications

Morphine interacts with brain serotonergic (5-HT) systems; these systems have been implicated in morphine analgesia and dependence (see Cervo et al., 1981). The 5-HT agonist quipazine induces analgesia in rats that is attenuated by naloxone and 5-HT antagonists (Minnema et al., 1980; Samanin et al., 1976). Behavioral disruption by the hallucinogens LSD, DMT and mescaline, mediated primarily through brain 5-HT effects (Rech and Commissaris, 1982), is potentiated by naloxone and naltrexone (Commissaris et al., 1980; Ruffing and Domino, 1981) and is variably antagonized or potentiated by morphine and methadone (Ruffing and Domino, 1981). Cyclazocine causes a disruption of operant behavior …

[3 Cortical Blood Flow And Oxygenation Abstracts], Y. Taguchi, M. Kaminogo, G. Austin

The Dr. George M. Austin Neurology Research Collection

Three presentations stapled together:

The Changes of Cortical Blood Flow and Cortical Oxygen Tension in Focal Ischemia. M. Kaminoga, M.D., Y. Taguchi, G. Austin, M.D. Conference presentation. American College of Surgeon Annual Meeting, January 28, 1984. NOTE 2nd page of this abstract not present in this file. 2nd page is present in Individual presentation posted separately.

Effect of Hypoxia and Hyperoxia of NADH Redox State and Cortical PO₂ in the Rabbit. Y Taguchi, M.D.; M. Kaminogo, M.D.; G. Austin, M.D. Conference Presentation. Southern California Chapter, American College of Surgeons, Santa Barbara, Calif, January 28, 1984.

Autoregulation of Cortical …

Effect Of Membrane Filtration Of Antimalarial Drug Solutions On In Vitro Activity Against Plasmodium Falciparum, J. Kevin Baird, C. Lambros

United States Public Health Resources

Antimalarial activities ofchloroquine, mefloquine, amodiaquine, and quinine in vitro against Plasmodium falciparum were diminished as a consequence ofmembranefiltration. Filtered drug solutions gave ID₅₀ values up to 25-fold greater than those of non-filtered (ethanol-sterilized) drug solutions. Loss ofactivity byfiltration was overcome by increasing the drug concentration prior to filtration. Water solutions filtered through Millex-GSfilter units consistently showed an absorbance maximum at 277 nm, accompanied by a lesser peak at 225 nm. Water filtrates from Nucleopore and Millex-G Vfilters showed no absorbance at 277 nm and only slight absorbance was evidentfor the Gelmanfilter unit. Activity losses were attributed to extractable contaminating …

The Effect Of Visible Light On Carotenogenesis Of Arthrosporulating Trichophyton Mentagrophytes, Ruth C. Mock

Dissertations

No abstract provided.

Activation Of B Lymphocytes By Monovalent Anti-Lyb-2 Antibodies, Subbarao Bondada, D. E. Mosier

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Small resting B lymphocytes are activated when their cell surface immuno-globulin (Ig) receptors interact with specific antigen or anti-Ig antibodies. Such a receptor-ligand interaction leads to enlargement and proliferation of B lymphocytes. These activated B cells can subsequently mature into Ig-secreting cells in the presence of appropriate accessory cell signals (1-3). However, monovalent anti-Ig antibodies cannot induce B cell activation even though they can bind to the surface Ig (4-7). It has thus been concluded that membrane Ig has to be cross-linked to induce early steps in B cell activation (membrane depolarization; 8) as well as later steps (proliferation). T …

The Incidence Of Obesity In Lds College Women: The Effect Of Selected Physical Socio-Environmental Variables On Total Percent Body Fat In Two Populations Of Lds Women, Carrie Tanner Summers

Theses and Dissertations

The purpose of this study was to determine the effect of selected physical socio-environmental variables on total percent body fat. The sample population consisted of single, LDS, white women attending Brigham Young University (BYU) and California State University at Fullerton (CSUF). Significant data as well as trends that appeared were included in this paper.

From the data collected, it was concluded that the sample populations at BYU and CSUF were the same. The incidence of obesity was determined only among individuals attending both universities. The entire sample population mean was 22.19 percent. This percentage did not meet the obesity criteria. …