Medicine and Health Sciences Commons^™

The Pharmacokinetics Of Nebulized Nanocrystal Budesonide Suspension In Healthy Volunteers., Walter Kraft, Barry Steiger, Don Beussink, John N. Quiring, Nancy Fitzgerald, Howard E. Greenberg, Scott A. Waldman

Department of Medicine Faculty Papers

Nanocrystal budesonide (nanobudesonide) is a suspension for nebulization in patients with steroid-responsive pulmonary diseases such as asthma. The pharmacokinetics and safety of the product were compared to those of Pulmicort Respules. Sixteen healthy volunteers were administered nanobudesonide 0.5 and 1.0 mg, Pulmicort Respules 0.5 mg, and placebo in a four-way, randomized crossover design. All nebulized formulations were well tolerated, with no evidence of bronchospasm. Nebulization times were significantly shorter for nanobudesonide compared to Pulmicort Respules. Because of a low oral bioavailability, plasma concentration of budesonide is a good marker of lung-delivered dose. The pharmacokinetics of nanobudesonide 0.5 and 1.0 mg …

Cd46-Mediated Transduction Of A Species D Adenovirus Vaccine Improves Mucosal Vaccine Efficacy, Zenaido T. Camacho, Mallory A. Turner, Michael A. Barry, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

The high levels of preexisting immunity against Adenovirus type 5 (Ad5) have deemed Ad5 unusable for translation as a human vaccine vector. Low seroprevalent alternative viral vectors may be less impacted by preexisting immunity, but they may also have significantly different phenotypes from that of Ad5. In this study we compare species D Ads (26, 28, and 48) to the species C Ad5. In vitro transduction studies show striking differences between the species C and D viruses. Most notably, Ad26 transduced human dendritic cells much more effectively than Ad5. In vivo imaging studies showed strikingly different transgene expression profiles. The …

Phylogenetic Analyses Of Texas Isolates Indicate An Evolving Subtype Of The Clade B Feline Immunodeficiency Viruses, Eric A. Weaver, Ellen W. Collisson, Margaret Slater, Guan Zhu

Nebraska Center for Virology: Faculty Publications

Rigorous phylogenetic analyses were used to compare the nucleotide sequences of feline immunodeficiency virus strains isolated from Texas and throughout the world. The envelope V3-V4 sequences and capsid gene of the Texas isolates formed a cluster between subtypes B and E. Statistical comparisons with other published sequences confirmed that the Texas group is a unique cluster, possibly a new subtype, arising from subtype B.

The Effect Of Hyperbaric Oxygen Therapy (Hbo) When Used Postoperatively In Skin Grafting Over Radiated Tissue In The Rat, Burçak Tümerdem, Ufuk Emekli̇, Burcu Çelet Özden, Şami̇l Aktaş, Mi̇sten Demi̇ryont, Gönül Kemi̇kler

Turkish Journal of Medical Sciences

Under conditions that may risk the success of flap surgery, free skin grafting may be an option for the reconstruction of the irradiated area. Clinical observations reveal that skin grafts applied to irradiated area undergo ulceration, resulting in chronic open wounds. The role of hyperbaric oxygen therapy (HBO) in problem wounds is to increase the tissue oxygen tension to optimize fibroblast proliferation and collagen synthesis, to enhance the oxidative killing capacity of the white blood cells and to stimulate angiogenesis. HBO has also been shown to stimulate angiogenesis in radiation-damaged tissue. In view of this data, an animal model is …

What Is New In Wound Healing?, Senthil Kumar, Peng Foo Wong, David John Leaper

Turkish Journal of Medical Sciences

Wound biology is complex. Wounds which were until recently seen only as defects in tissues are now increasingly interpreted in cellular and molecular terms. Growth factors, cytokines, proteases and adhesion molecules which participate in wound healing are discussed in this article. From a clinical perspective, conceptual shifts of importance, including moist wound healing, wound bed preparation and wound assessment, are presented. The frontiers of therapeutics employed in wound healing continue to advance with an increasing array of modalities joining the ranks at a regular pace. A range of currently available as well as evolving therapies- physical (topical negative pressure therapy, …

Mesenchymal Chondrosarcoma Of The Mandible, Nusret Akpolat, Üzeyi̇r Gök

Turkish Journal of Medical Sciences

KPOLAT^1, Üzeyir GÖK^2 ^1Department of Pathology, Faculty of Medicine, Fırat University, Elazığ - Turkey ^1Department of Otorhinolaryngology, Faculty of Medicine, Fırat University, Elazığ - Turkey

Systemic Responses To Burn Injury, Bariş Çakir, Berrak C. Yeğen

Turkish Journal of Medical Sciences

The major causes of death in burn patients include multiple organ failure and infection. It is important for the clinician to understand the pathophysiology of burn injury and the effects it will have on the pharmacokinetics of a drug. The local and systemic inflammatory response to thermal injury is extremely complex, resulting in both local burn tissue damage and deleterious systemic effects on all other organ systems distant from the burn area itself. Thermal injury initiates systemic inflammatory reactions producing burn toxins and oxygen radicals and finally leads to peroxidation. The relationship between the amount of products of oxidative metabolism …

Genotoxic Evaluation Of The Antibacterial Drug, Ciprofloxacin, In Cultured Lymphocytes Of Patients With Urinary Tract Infection, Mevli̇t İkbal, Hasan Doğan, Hati̇ce Odabaş, İbrahi̇m Pi̇ri̇m

Turkish Journal of Medical Sciences

Ciprofloxacin is a quinolone carboxylic acid derivative and is commonly used in medicine. The genotoxicity of ciprofloxacin was evaluated in cultured human peripheral blood lymphocytes in patients with urinary tract infection. Sister chromatid exchange (SCE), mitotic index (MI) and replicative index (RI) were measured before and after ciprofloxacin therapy. Our results showed that SCE frequency significantly increased after ciprofloxacin therapy (P < 0.001), but MI and RI decreased (P < 0.001). The results of this study suggest that clinical therapy using ciprofloxacin may have a moderate genotoxic potential.

Analysis Of Borrelia Burgdorferi Antibody Seroprevalence In Patients With Behçet´S Disease Using 3 Different Serological Assays, Ergi̇n Ayaşlioğlu, Emel Erkek, Sedat Kaygusuz, Di̇lek Kiliç, Ali̇ İnal, Göksal Keski̇n

Turkish Journal of Medical Sciences

Behçet´s disease is a chronic multisystemic inflammatory vasculitis of unknown etiology. Various infectious agents have been implicated as potential triggering factors in the pathogenesis of the disease. The present study was designed to evaluate the seroprevalence of Borrelia burgdorferi antibodies in Turkish patients with Behçet´s disease. For this purpose, 90 consecutive patients with Behçet´s disease and 50 healthy individuals were enrolled into a prospective study. The IgM and IgG antibodies to B. burgdorferi in sera were determined by 3 different serological techniques, namely indirect immunofluorescence assay (IFA), enzyme linked immunosorbent assay (ELISA) and Western blotting (WB). Although there was a …

Thermodynamic Consequences Of Dna Base Modifications And Ligand-Dna Interactions., Rachel Hutchins Graves

All ETDs from UAB

The thermodynamic effects of alterations of the DNA duplex by DNA base modifications or ligand binding are investigated by a combination of spectrophotometric and calorimetric methods. Examination of the effects of substituent modification on the DNA binding energetics of the anticancer agent AAC (Y-[2-(dimethylamino)ethyl]-9-aminoacridine-4-carboxamide) indicated that DNA binding affinities and energetics were enhanced upon substitution at the C5 position on the chromophore of the drug with a variety of chemical groups. A correlation between enthalpies of DNA binding and anticancer activity was evident.The impacts of DNA base modifications, including cytosine arabinoside, deaminated cytosine, and abasic sites, on the thermodynamics of …

Faculty Publications 2003, Lsu Health Sciences Center New Orleans

Historical Faculty Publications

This Faculty bibliography contains 869 publications.

Multi-Channel Fetal Magnetocardiographic Signal Seperation For Analysis Of Heart Rate Variability, Joshua Quaid Campbell

Theses and Dissertations

Objective: The objectives were to implement signal-processing techniques to separate the fetal magnetocardiogram (FMCG), as collected by SQUID (Superconducting QUantum Interference Device) gradiometers, from mixtures of maternal and fetal biomagnetic signals recorded during the antepartum period and to develop a database of normal FMCG values of the fetal cardiac time intervals and heart rate variability measures. Materials and Methods: The research was performed in three phases: 1) Independent component analysis (ICA), nonlinear filtering techniques, and orthogonal projection methods were optimized enabling the FMCG to be processed using traditional signal analysis techniques; 2) The cardiac time intervals and heart rate variability …

A Drosophila Protein-Interaction Map Centered On Cell-Cycle Regulators, Clement A. Stanyon, Guozhen Liu, Bernardo A. Mangiola, Nishi Patel, Loic Giot, Bing Kuang, Huamei Zhang, Jinhui Zhong, Russell L. Finley Jr

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Maps depicting binary interactions between proteins can be powerful starting points for understanding biological systems. A proven technology for generating such maps is high-throughput yeast two-hybrid screening. In the most extensive screen to date, a Gal4-based two-hybrid system was used recently to detect over 20,000 interactions among Drosophila proteins. Although these data are a valuable resource for insights into protein networks, they cover only a fraction of the expected number of interactions.

Results

To complement the Gal4-based interaction data, we used the same set of Drosophila open reading frames to construct arrays for a LexA-based two-hybrid system. We …

Imaging Genome Abnormalities In Cancer Research, Henry Hq Heng, Joshua B. Stevens, Guo Liu, Steven W. Bremer, Christine J. Ye

Wayne State University Associated BioMed Central Scholarship

Abstract

Increasing attention is focusing on chromosomal and genome structure in cancer research due to the fact that genomic instability plays a principal role in cancer initiation, progression and response to chemotherapeutic agents. The integrity of the genome (including structural, behavioral and functional aspects) of normal and cancer cells can be monitored with direct visualization by using a variety of cutting edge molecular cytogenetic technologies that are now available in the field of cancer research. Examples are presented in this review by grouping these methodologies into four categories visualizing different yet closely related major levels of genome structures. An integrated …

Traumatic Deep Vein Thrombosis In A Soccer Player: A Case Study, Paul S. Echlin, Ross Eg Upshur, Douglas B. Mckeag, Harsha P. Jayatilake

Wayne State University Associated BioMed Central Scholarship

Abstract

A 42 year-old male former semi-professional soccer player sustained a right lower extremity popliteal contusion during a soccer game. He was clinically diagnosed with a possible traumatic deep vein thrombosis (DVT), and sent for confirmatory tests. A duplex doppler ultrasound was positive for DVT, and the patient was admitted to hospital for anticoagulation (unfractionated heparin, warfarin). Upon discharge from hospital the patient continued oral warfarin anticoagulation (six months), and the use of compression stockings (nine months). He followed up with his family doctor at regular intervals for serial coagulation measurements, and ultrasound examinations. The patient's only identified major thrombotic …

Molecular Genetic Investigation Of Autosomal Dominant Hemifacial Microsomia, Rebecca Watt

Theses: Doctorates and Masters

The overall purpose of this project was to explore the underlying pathogenesis of Hemifacial microsomia (HFM), investigate treatment and management options identify likely candidate genes and screen candidate genes for mutation(s) causing the form of HFM segregating on chromosome 11 in a West Australian family. Hemifacial microsomia is a congenital malformation arising from the derivatives of the first and second branchial arches. It is both clinically and genetically heterogeneous and can occur sporadically or segregate within families in an autosomal fashion. HFM is characterised by significant undergrowth to one side of the face and is a common birth defect with …

Target Selection For Antisense Oligonucleotide Induced Exon Skipping In The Dystrophin Gene, Stephen J. Errington

Theses: Doctorates and Masters

Duchene Muscular Dystrophy (DMD), and the milder allelic Becker muscular dystrophy (EMD), are X-linked recessive muscle wasting disorders characterised by mutations in the dystrophin gene. DMD occurs at a frequency of approximately 1 in 3500 male newborns and life expectancy is typically less than 30 years. Due to progressive muscle wasting, affected boys are restricted to a wheelchair by the age of 12 years. The most common cause of death is pneumonia, compounded by cardiac involvement. Mutations that disrupt the dystrophin reading frame, or prevent the synthesis of either terminus, preclude the synthesis of a fully functional dystrophin. The subsequent …

Response Of Dark-Adapted Retinal Rod Photoreceptors, H. Khanal, V. Alexiades, E. Dibenedetto

Publications

The process of phototransduction, whereby light is converted into an electrical response, in rod and cone photoreceptors in the retina, involves as a key setp, the diffusion of the cytoplasmic, signaling molecules cGMP (cyclic guanosime monophosphate) and Ca²+ diffuse in the cytoplasm (the fluid surrounding the discs). the complex geometry of the rod creates computational difficulties. We present spatio-temporal compuational models for interacctions and diffusion of cGMP and Ca²+ in the cytoplasm of vertebrate rod photoreceptors, as well as numerical simulations fo the response to light of dark-adapted Salamander rods.

Adenoid Cystic Carcinoma Of The External Auditory Canal With Pulmonary Metastasis, Bumi̇n Deği̇rmenci̇, Ayli̇n Yücel, Murat Acar, Ramazan Albayrak

Turkish Journal of Medical Sciences

ĞİRMENCİ, Aylin YÜCEL, Murat ACAR, Ramazan ALBAYRAK Department of Radiology, Faculty of Medicine, Kocatepe University, Afyon - TURKEY

Use Of Spongious Bone Chips And Fascia Temporalis In Alveolar Bone Defects, Hakan H. Tüz, Ümi̇t K. Akal, Mi̇ne Cambazoğlu, Reha Ş. Ki̇şni̇şçi̇

Turkish Journal of Medical Sciences

Graft materials are used for inducement of regeneration in bone defects. Organic and synthetic bone graft materials facilitate remodelation or healing of the bone and induce new bone formation in the area of bone resorption caused by pathological, traumatic, and physiological reasons. The aim of this study was to evaluate the effects of spongious allogenic bone graft and fascia temporalis membranous collagen tissue on the healing of bone defects clinically and radiologically. The study was carried out on 90 bone defects of 81 patients who underwent apicoectomy, cystectomy, and curettage of chronic infections in the maxilla and mandible. Defects were …

Liesegang Rings In Breast Tissue: An Unusual Component Of A Foreign Body Reaction, Banu Si̇s, Tülay Canda, Ömer Harmancioğlu

Turkish Journal of Medical Sciences

^1, Tülay CANDA^1, Ömer HARMANCIOĞLU^2 ^1Department of Pathology, Faculty of Medicine, Dokuz Eylül University, İzmir - Turkey ^2Department of General Surgery, Faculty of Medicine, Dokuz Eylül University, İzmir - Turkey

Comparative Cytotoxicity Of Fludarabine In Human Peripheral Blood Mononuclear Cells And In The Promyelocytic Leukemia Cell Line (Hl60), A. Lale Doğan

Turkish Journal of Medical Sciences

Fludarabine is an inhibitor of DNA/RNA synthesis with antitumor activity on lymphoproliferative malignancies. We investigated the dose-dependent cytotoxic effects of the drug on human peripheral blood mononuclear cells, the HL60 cell line and the differentiated HL60 cells. Dimethylformamide was used for the neutrophilic differentiation of HL60 cells and the MTT assay was used to estimate drug cytotoxicity. Undifferentiated tumor cells had higher proliferation rates compared to the differentiated subline. There was 57% cell death in HL60 cells at a dose of 5 µg/ml of the drug. On the other hand, in differentiated HL60 cells and nonmalignant cells, cell death did …

Sister Chromatid Exchange Frequency In Lymphocytes Cultured From Cotton Gin Workers, Münevver Atmaca, Hüseyi̇n Bağci, İbrahi̇m Açikbaş, Di̇li̇han Gümüş, Füsun Düzcan

Turkish Journal of Medical Sciences

Genetic biomonitoring of human populations exposed to potential mutagens/carcinogens can be performed using different genetic markers. Sister chromatid exchange (SCE) is one of the most extensively used markers of the early biological effects of DNA damaging agents. In order to assess the genotoxicity associated with exposure to cotton dust, we determined SCE frequency in peripheral blood lymphocytes cultured from 20 cotton gin workers and 20 controls. Student’s-t test indicated an increased frequency of SCE in lymphocytes of the workers (14.66 ± 4.18) compared to the controls (10.40 ± 1.37) (P < 0.0001). The Mann-Whitney U test indicated that the duration of exposure to cotton dust (10 years) and the work area were not related to the frequency of SCE (P > 0.05). The textile industry is rapidly expanding in Turkey, and …

Prevalence Of Listeria Species In Ice Creams Sold In The Cities Of Kahramanmaraş And Adana, Deni̇z Akman, Ni̇zami̇ Duran, Meti̇n Diğrak

Turkish Journal of Medical Sciences

In this study, the prevalence of Listeria spp. was investigated in the ice cream samples collected from the downtown stores of Kahramanmaras (28 samples) and Adana (30 samples). A total of 58 ice cream samples were analysed by the Food and Drug Administration (FDA) two-stage enrichment techniques. Growth of Listeria spp. was determined in 14 (24.1%) of the samples collected from Kahramanmaras and 10 (17.2%) samples from Adana. The results of the biochemical tests revealed that the bacterial growth in the samples from Kahramanmaras was Listeria grayi whereas 8 (13.8%) samples of Adana were Listeria grayi, the other two samples …

The Evaluation Of The Stomatognathic System Of A Group With Anterior Openbite, Semra Ci̇ğer, Eli̇f İdi̇l Keser

Turkish Journal of Medical Sciences

Anterior openbite (AOB) is a malocclusion considered to be associated with symptoms and signs of temporomandibular disorders (TMD). An accurate, objective and repeatable method is needed in order to clarify the relationship between TMD and anterior openbite. The aim of this study is to examine the functions of the stomatognathic systems of 21 adolescents with AOB by means of electrovibratography (EVG), electrognathography (EMG), electroganthography (EGN), and transcranial and cephalometric radiographs. Nonconcentricity and mild asymmetry of the condyle fossa relationships were observed on transcranial radiographs. EMG results showed that 85% of the subjects had abnormal swallowing patterns and 52% had abnormal …

Case Report: Intramesocolic Malposition Of The Gallbladder, Oral Saygun, Kuzey Aydinuraz, Çağatay Daphan, Abdullah Akkus, Fati̇h Ağalar

Turkish Journal of Medical Sciences

GUN, Kuzey AYDINURAZ, Cağatay DAPHAN, Abdullah AKKUS, Fatih AĞALAR Department of General Surgery, Faculty of Medicine, Kırıkkale University, Kırıkkale - TURKEY

Can The Natural Carbonic Anhydrase Inhibitors In Trout Plasma Be Used In The Treatment Of Some Human Diseases?, Şükrü Beydemi̇r, Olcay Hi̇sar

Turkish Journal of Medical Sciences

No abstract provided.

The Effects Of Melatonin On 6-Phosphogluconate Dehydrogenase: An In Vitro And In Vivo Study, İlhami̇ Gülçi̇n, Şükrü Beydemi̇r

Turkish Journal of Medical Sciences

Melatonin is known to influence a variety of biological processes including circadian rhythms, neuroendocrine, and cardiovascular and immune functions as well as thermoregulation. Melatonin is the chief secretory product of the pineal gland, although it is also produced in other organs. In this study, in vitro effects of melatonin on 6-phosphogluconate dehydrogenase from human erythrocytes and in vivo effects of melatonin on 6-phosphogluconate dehydrogenase from rat (Sprague-Dawley) erythrocytes were studied. Human erythrocyte 6-phosphogluconate dehydrogenase was purified in 3 steps, namely haemolysate preparation, ammonium sulphate fractionation (35-65%) and 2´,5´-ADP Sepharose-4B affinity gel chromatography. 6-Phosphogluconate dehydrogenase was purified with a recovery rate …

The Effects Of Asthma Education On Knowledge, Behavior And Morbidity In Asthmatic Patients, Rezan Demi̇ralay

Turkish Journal of Medical Sciences

This work was carried out to study the effect of asthma education on asthma knowledge, behavior and morbidity in asthmatic patients. A randomized double blind controlled study was conducted with 54 adults with asthma followed-up at the outpatient clinic of Chest Diseases and Tuberculosis at the Faculty of Medicine of Suleyman Demirel University. Patients were randomized to the verbal (n: 17), written (n: 19), and verbal-written (n: 18) education groups. Knowledge of asthma was measured at the baseline, and 2 months and 1 year after education. Compliance with treatment, correct inhaler skill and admisssons to hospital in the previous year …

Abdominal Wall Endometrioma: Localizing In Rectus Abdominus Sheath, Mustafa Çeli̇k, Ertan Bülbüloğlu, Mehmet Aki̇f Büyükbeşe, Ali̇ Çeti̇nkaya

Turkish Journal of Medical Sciences

ÇELİK^1, Ertan BÜLBÜLOĞLU^2, Mehmet Akif BÜYÜKBEŞE^3, Ali ÇETİNKAYA^3 ^1Department of Family Medicine, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş - Turkey ^2Department of General Surgery, Faculty of Medicine, Kahramanmaraş Sütçü İmam University , Kahramanmaraş - Turkey ^3Department of Internal Medicine, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş - Turkey