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Articles 39301 - 39330 of 42084

Full-Text Articles in Medicine and Health Sciences

Partial Purification And Characterization Of F₄₂₀-Dependent Nadp Reductase From Methanobrevibacter Smithii Strain De1, Scott D. Sheridan Jan 1985

Partial Purification And Characterization Of F₄₂₀-Dependent Nadp Reductase From Methanobrevibacter Smithii Strain De1, Scott D. Sheridan

Dissertations and Theses

The F420-dependent NADP reductase of Methanobrevibacter smithii has been partially purified employing a combination of affinity chromatography with Blue Sepharose (Cl-6B) and molecular sieve chromatography with Sephacryl S-200, The enzyme, which requires reduced F420 as an electron donor, has been purified over 145 fold with a recovery of 6%. A molecular weight of 120,00 for the native enzyme was determined by Sephacryl S-200 chromatography. A subunit molecular weight of 28,200 was determined by SDS-PAGE, indicating that the native enzyme is a tetramer. The optimal temperature for enzymatic activity was found to be 45°C, with a pH optimum …

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Methemoglobin Reduction In The Presence Of Various Concentrations Of Oxyhemoglobin, Ali Mansouri Jan 1985

Methemoglobin Reduction In The Presence Of Various Concentrations Of Oxyhemoglobin, Ali Mansouri

Journal of the Arkansas Academy of Science

Methemoglobin reduction in human red blood cell is governed by many factors, one of which is the concentration of oxyhemoglobin. Experiments were conducted to determine the inhibition of methemoglobin reduction by oxyhemoglobin under concentrations approaching those in the red cell. Hemoglobin, methemoglobin reductase and cytochrome b₅ were prepared by routine protein purification methods. Methemoglobin reduction was carried out at 37° C in spectrophotometer. It is shown that methemoglobin concentration variation (at least when it is above 1 mM) does not change the reduction rate. Whereas, the ratio of methemoglobin to oxyhemoglobin determines the rate of methemoglobin reduction when all other …

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Fungicidal Control Of Wheat Leaf Diseases, A G P Brown Jan 1985

Fungicidal Control Of Wheat Leaf Diseases, A G P Brown

Experimental Summaries - Plant Research

In a further experiment on fungicides to protect wheat during flowering and grain fill from Septoria nodorurn at Badgingarra Research Station, rates below 1 litre and lower carrier volumes were compared for the fungicide Tilt applied at Zadoks stage 50. A few other fungicides were also included. At spraying there was a moderate infection by Septoria nodorurn (85% of leaf damage), Septoria tritici (10%) and Pyrenophora tritici-repentis (5%). Good control was obtained with most treatments (Table 1) with only Benlate at 1 kg/ha appearing ineffective when percentage disease was rated on October 10, 1985. At October 17 disease scores differed …

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Barley Agronomy., K. J. Young, B. R. Wylde, B. Raszyk, T. N. Khan Jan 1985

Barley Agronomy., K. J. Young, B. R. Wylde, B. Raszyk, T. N. Khan

Experimental Summaries - Plant Research

Barley foliar diseases, effect on nitrogen response, 85ES25, 85E24, 85E25, 85MT37, 85ES29, 85MT38, 85E26, 85ES32, 85ES31, 85ES30, 85ES26, 85ES24, 85ES27, 85ES28, 85ES34, 85M27, 85SG27, 85MT42, 85C70,

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Low Tillering Cereals, Early Maturity Of Cereals, Cereal Variety And Reduced Branching Lupins., R. Delane, J. Hamblin Jan 1985

Low Tillering Cereals, Early Maturity Of Cereals, Cereal Variety And Reduced Branching Lupins., R. Delane, J. Hamblin

Experimental Summaries - Plant Research

Trial 85C62 - growth and water use of low tillering cereals. Trial 85C62 - yield potential of low tillering cereals. Trial WEUNI - response of low tillering breeding lines and standard varieties to sowing rate. Trial 85C45 - effect of flowering date, seeding rate, deep tillage and applied nitrogen on yield of barley. Trial 85C61 - wheat variety x management interaction on sandplain. Trial 85C55 - growth and water use of reduced branching lupins. Trial 85C56 - normal and reduced branching lupins - planting density response.

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Modification Of Sand Fly Biting Behavior By Leishmania Leads To Increased Parasite Transmission, Raymond Beach, Gabriel Kjilu, Johannis Leeuwenberg Jan 1985

Modification Of Sand Fly Biting Behavior By Leishmania Leads To Increased Parasite Transmission, Raymond Beach, Gabriel Kjilu, Johannis Leeuwenberg

Harold W. Manter Laboratory of Parasitology: Library Materials

To attempt rodent-sand fly-rodent transmission of Leishmania major, laboratory-reared Phlebotomus doboscqi were fed on L. major-infected mice and then refed on uninfected mice 21 days later. Flies which refed either probed 1–2 times and took a full blood meal in less than 10 minutes or probed 3 or more times and took little or no blood during a period of 15 minutes or more. When dissected, 7 of 8 flies which experienced difficulty in obtaining a blood meal had flagellates in their cibaria, an observation supporting the hypothesis that parasites in this part of the alimentary canal modify normal …

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Sulfite Residues In Maraschino Cherries, Julie A. Nordlee, Laura B. Martin, Steve L. Taylor Jan 1985

Sulfite Residues In Maraschino Cherries, Julie A. Nordlee, Laura B. Martin, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

A survey of 53 samples of maraschino cherries from 14 different processors revealed that total sulfite residues averaged 52.3 ± 44.7 ppm. However, 58.5% of the samples had less than 40 ppm total SO2, while only 7.6% had greater than 120 ppm total SO2 indicating that the distribution was skewed in the direction of lower residue levels. Free sulfite residues in the 53 samples were considerably lower, averaging 14.2 ± 7.1 ppm. With free SO2 levels, 35.8% of the samples had less than 10 ppm free SO2, while only 5.7% had greater than 30 …

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Knox-Wise Family Papers - Accession 591, Knox-Wise Jan 1985

Knox-Wise Family Papers - Accession 591, Knox-Wise

Manuscript Collection

The Knox-Wise Family Papers includes a land grant issued to John Knox in 1768; diaries written by Dr. John Knox [1792-1859] covering the 1840s and 1850s; James N. Knox [1806-1880] covering 1859-1880; and William D. Knox [1847-1928] covering 1869-1928; indentures, deeds, receipts, court summonses and other papers of Hugh Knox [1757-1821], sheriff and justice of the peace in Chester County, South Carolina (ca. 1780s and 1790s); correspondence of James N. Knox, correspondence, and other professional papers of Dr. John Knox; correspondence, and other papers of William D. Knox, Superintendent of Education in Chester County from 1896-1928. Papers of various other …

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Animals In Testing: How The Cpi Is Handling A Hot Issue, Anonymous Dec 1984

Animals In Testing: How The Cpi Is Handling A Hot Issue, Anonymous

Popular Press Items

No abstract provided.

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Linkage Data Excluding A Locus For Multiple Endocrine Neoplasia Type 2 Syndromes From The Distal Part Of The Short Arm Of Chromosome 11, Kenneth K. Kidd, Susan D. Kruger, Daniela S. Gerhard, Judith R. Kidd, David Housman, Joseph M. Gertner Dec 1984

Linkage Data Excluding A Locus For Multiple Endocrine Neoplasia Type 2 Syndromes From The Distal Part Of The Short Arm Of Chromosome 11, Kenneth K. Kidd, Susan D. Kruger, Daniela S. Gerhard, Judith R. Kidd, David Housman, Joseph M. Gertner

Henry Ford Hospital Medical Journal

A study designed to identify the location of a gene for multiple endocrine neoplasia type 2 (MEN-2) is being performed using a single large family segregating for MEN-2A. Here we report our initial results using a set of DNA restriction fragment length polymorphisms spanning a 40 cM segment of the short arm of human chromosome 11. The locus for MEN-2 in this family is excluded from this entire region which includes an oncogene, c-Ha-ras-1, two hormone loci, insulin and parathyroid, and the five hemoglobin genes in the beta hemoglobin gene duster. This is the first exclusion of such a large …

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The First International Workshop On Multiple Endocrine Neoplasia Type 2 Syndromes, Charles E. Jackson Dec 1984

The First International Workshop On Multiple Endocrine Neoplasia Type 2 Syndromes, Charles E. Jackson

Henry Ford Hospital Medical Journal

No abstract provided.

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Impact Of Screening On Prognosis In The Multiple Endocrine Neoplasia Type 2 Syndromes: Natural History And Treatment Results In 105 Patients, Margareta Telenius-Berg, Bertel Berg, Bertil Hamberger, Sten Tibblin, Lars-Erik Tisell, Lars Ysander, Gunnar Welander Dec 1984

Impact Of Screening On Prognosis In The Multiple Endocrine Neoplasia Type 2 Syndromes: Natural History And Treatment Results In 105 Patients, Margareta Telenius-Berg, Bertel Berg, Bertil Hamberger, Sten Tibblin, Lars-Erik Tisell, Lars Ysander, Gunnar Welander

Henry Ford Hospital Medical Journal

We evaluated the effects of screening for multiple endocrine neoplasia type 2A (MEN-2A) in 12 families. Genealogical studies going back to 1730 show a common ancestry for seven Swedish families and one American family. The total number of patients included 105 individuals, 68 of whom were diagnosed by our screening program. Our screening methods for medullary carcinoma of the thyroid (MTC) had an optimal sensitivity and specificity. The frequency of gene carriers detected in MEN-2A families was 55%. Screening will lead to early diagnosis and early therapy, which in turn, will significantly decrease morbidity, incidence of surgical complications, and mortality …

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Central Registration Of Multiple Endocrine Neoplasia Type 2 Families In The Netherlands, C. J. M. Lips, E. Den Aantrekker, J. M. Jansen-Schillhorn Van Veen, R. A. Geerdink, G. Griffioen, E. A. Van Slooten Dec 1984

Central Registration Of Multiple Endocrine Neoplasia Type 2 Families In The Netherlands, C. J. M. Lips, E. Den Aantrekker, J. M. Jansen-Schillhorn Van Veen, R. A. Geerdink, G. Griffioen, E. A. Van Slooten

Henry Ford Hospital Medical Journal

No abstract provided.

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Chromosomes In Multiple Endocrine Neoplasia Type 2 Syndromes, Daniel L. Van Dyke, V. Ramesh Babu, Charles E. Jackson Dec 1984

Chromosomes In Multiple Endocrine Neoplasia Type 2 Syndromes, Daniel L. Van Dyke, V. Ramesh Babu, Charles E. Jackson

Henry Ford Hospital Medical Journal

In 19 patients from nine MEN-2A families, high-resolution C-banded chromosome studies have revealed a visible deletion within sub-band 20p12.2, yet no abnormality was observed in a 10th family. A deletion indistinguishable from that in MEN-2A was observed in five patients from three MEN-2B families but not in two other MEN-2B families. We found no abnormality in the entire karyotype of the four MEN-1 patients studied. These findings suggest that the mutation in most MEN-2 patients is a visible deletion in the short arm of chromosome 20.

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Research Vistas In The Multiple Endocrine Neoplasia Syndromes, John J. Mulvihill Dec 1984

Research Vistas In The Multiple Endocrine Neoplasia Syndromes, John J. Mulvihill

Henry Ford Hospital Medical Journal

Progress in understanding the single gene, cytogenetic, and multifactorial traits that predispose to human cancer suggests possible new directions for research in the multiple endocrine neoplasia (MEN) syndromes. Among the other 200 or so monogenic disorders associated with human neoplasia, advances have come from further delineation of syndromes by various clinical specialists, the recognition of subtypes of syndromes previously thought to be homogeneous, the search for in vitro manifestations of the mutant gene in fibroblasts, and the establishment of cell, tissue and patient registries and of voluntary lay organizations to serve as advocates for the disease. With regard to cytogenetics, …

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Book Review: Medical Meanings, Fred W. Whitehouse Dec 1984

Book Review: Medical Meanings, Fred W. Whitehouse

Henry Ford Hospital Medical Journal

No abstract provided.

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Front Matter Dec 1984

Front Matter

Henry Ford Hospital Medical Journal

No abstract provided.

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Multiple Endocrine Neoplasia Type 2 Syndromes: Historical Perspectives, John H. Sipple Dec 1984

Multiple Endocrine Neoplasia Type 2 Syndromes: Historical Perspectives, John H. Sipple

Henry Ford Hospital Medical Journal

No abstract provided.

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The Calcitonin Assay And Multiple Endocrine Neoplasia Type 2 Syndromes: Historical Footnote, Kenneth E. W. Melvin Dec 1984

The Calcitonin Assay And Multiple Endocrine Neoplasia Type 2 Syndromes: Historical Footnote, Kenneth E. W. Melvin

Henry Ford Hospital Medical Journal

No abstract provided.

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Register Of Multiple Endocrine Neoplasia Type 2 Syndromes In The United Kingdom, Bruce A. J. Ponder Dec 1984

Register Of Multiple Endocrine Neoplasia Type 2 Syndromes In The United Kingdom, Bruce A. J. Ponder

Henry Ford Hospital Medical Journal

A collaborative group for the study of multiple endocrine neoplasia type 2 (MEN-2) syndromes comprised of clinicians and laboratory scientists has been set up in the United Kingdom. Its aims are 1) to provide a basis for collaborative work on MEN-2; 2) to establish a register of patients; 3) and specifically to conduct studies aimed at defining the best policy for screening the families of apparently sporadic patients, to establish radioimmunoassays for family screening, and to identify large kindreds for genetic linkage studies using DNA polymorphisms.

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Screening For Hereditary Medullary Cancer In Denmark, Kristian Emmertsen Dec 1984

Screening For Hereditary Medullary Cancer In Denmark, Kristian Emmertsen

Henry Ford Hospital Medical Journal

Screening of first-degree relatives of patients with medullary thyroid cancer (MTC) gave normal values of pentagastrin-stimulated serum calcitonin and 24-hour urinary catecholamine levels in the relatives of 18 of 22 patients. This result is considered to be valid evidence for sporadic MTC. Absence of C-cell hyperplasia maybe another indication of sporadic MTC. Four hereditary MTCs were represented by one fully expressed MEN-2B patient without affected relatives, one fully expressed MEN-2A case in one family, and two first cousins with MTC as the only MEN-2A lesion in another family. Fourteen relatives of the MEN-2A patients had elevated serum calcitonin levels, and …

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Familial Medullary Carcinoma Of The Thyroid: Clinical Studies In Northern New England, Walter W. Noll, L. Herbert Maurer, Victoria L. Herzberg, Paul J. Beisswenger, Brian M. Quinn, Charles C. Cate, Jane P. Bassick, Patricia A. Clark, Thomas A. Colacchio Dec 1984

Familial Medullary Carcinoma Of The Thyroid: Clinical Studies In Northern New England, Walter W. Noll, L. Herbert Maurer, Victoria L. Herzberg, Paul J. Beisswenger, Brian M. Quinn, Charles C. Cate, Jane P. Bassick, Patricia A. Clark, Thomas A. Colacchio

Henry Ford Hospital Medical Journal

No abstract provided.

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Multiple Endocrine Neoplasia Type 2 Syndromes In Japan, Shin-Ichiro Takai, Akira Miyauchi, Hideo Matsumoto, Tatsuro Ikeuchi, Tetsuro Miki, Kanji Kuma, Yuichi Kumahara Dec 1984

Multiple Endocrine Neoplasia Type 2 Syndromes In Japan, Shin-Ichiro Takai, Akira Miyauchi, Hideo Matsumoto, Tatsuro Ikeuchi, Tetsuro Miki, Kanji Kuma, Yuichi Kumahara

Henry Ford Hospital Medical Journal

Through nationwide surveys, we collected and analyzed 242 patients of medullary thyroid carcinoma (MTC). Included were 40 patients with multiple endocrine neoplasia type 2A (MEN-2A), six patients with MEN-2B, and 36 patients with only MTC having a positive family history (82 total patients in the hereditary group). Ten-year survival rates were 81.5% for all cases, and 97.5% and 76.1% for the hereditary and the sporadic group, respectively. Epinephrine/norepinephrine ratio in the urine was found to be a good indicator of the adrenomedullary hyperfunction in patients with hereditary MTC. At least one patient in each family with hereditary MTC had overt …

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A French Canadian Family With Multiple Endocrine Neoplasia Type 2 Syndromes, Maurice B. Verdy, Marcel Cadotte, Walter Schurch, William C. Sturtridge, Jacques Cantin, Andrée M. Weber, André Lacroix, Cynthia Forster-Gibson Dec 1984

A French Canadian Family With Multiple Endocrine Neoplasia Type 2 Syndromes, Maurice B. Verdy, Marcel Cadotte, Walter Schurch, William C. Sturtridge, Jacques Cantin, Andrée M. Weber, André Lacroix, Cynthia Forster-Gibson

Henry Ford Hospital Medical Journal

No abstract provided.

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Scintigraphy With 1-131 Mibg As An Aid To The Treatment Of Pheochromocytomas In Patients With The Multiple Endocrine Neoplasia Type 2 Syndromes, James C. Sisson, Brahm Shapiro, William H. Beierwaltes Dec 1984

Scintigraphy With 1-131 Mibg As An Aid To The Treatment Of Pheochromocytomas In Patients With The Multiple Endocrine Neoplasia Type 2 Syndromes, James C. Sisson, Brahm Shapiro, William H. Beierwaltes

Henry Ford Hospital Medical Journal

We reviewed the scintigraphic images made after injections of 1-131 metaiodobenzylguanidine (MIBG) or 1-123 MIBG in patients with multiple endocrine neoplasia (MEN) types 2A and 2B. The information we obtained was applied to three questions about the treatment of pheochromocytoma in patients affected with these syndromes. Our first question "When should adrenal gland(s) be removed?" was not directly answered. However, adrenalectomy generally should not be contemplated unless distinct abnormalities are present in the scintigraphic images. With experience it may be possible to approximate, from the stage of pheochromocytoma depicted by scintigraphy, how many years will elapse before symptoms or hypertension …

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The Molecular Basis Of Polypeptide Hormone Production By Medullary Thyroid Cancer, C. J. M. Lips, P. H. Steenbergh, J. W. M. Höppener, J. Zandberg, H. S. Jansz Dec 1984

The Molecular Basis Of Polypeptide Hormone Production By Medullary Thyroid Cancer, C. J. M. Lips, P. H. Steenbergh, J. W. M. Höppener, J. Zandberg, H. S. Jansz

Henry Ford Hospital Medical Journal

Using recombinant DNA techniques, we analyzed the structure of human calcitonin (CT)-encoding DNA. The sequence of nucleotides derived from the messenger RNA (mRNA) predict the amino acid sequence of the entire human CT precursor protein. The complete CT gene could be isolated from a DNA library of the human genome. The CT precursor encoding sequences (exons) as well as the noncoding intervening sequences (introns) could be identified. Another sequence, called human calcitonin gene-related peptide (hCCRP), could be located near this CT-encoding DNA. A genomic fragment encoding hCCRP was used as a probe to detect and isolate the corresponding mRNA in …

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Genetic Studies Of Multiple Endocrine Neoplasia Type 2 Syndromes: A Workshop Commentary, Nancy E. Simpson Dec 1984

Genetic Studies Of Multiple Endocrine Neoplasia Type 2 Syndromes: A Workshop Commentary, Nancy E. Simpson

Henry Ford Hospital Medical Journal

No abstract provided.

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Back Matter Dec 1984

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

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Skin Temperature Changes In The Ankle During And After Ice Pack Application Of 10, 20, 30, 45, And 60 Minutes, Joan H. Mlynarczyk Dec 1984

Skin Temperature Changes In The Ankle During And After Ice Pack Application Of 10, 20, 30, 45, And 60 Minutes, Joan H. Mlynarczyk

All-Inclusive List of Electronic Theses and Dissertations

This study investigated changes in ankle skin temperature during and following various durations of therapeutic cold application. Surface temperature was measured with a telethermister on the ankles of 10 male subjects during five treatment conditions. Experimental conditions involved the application of an ice pack to the ankle for 10, 20, 30, 45, and 60 minutes. Each subject was tested under all five treatment conditions utilizing a balanced Latin Square design. Subjects were required to lie at rest in a supine manner with both ankles elevated six inches above the treatment table. Following a 30 minute pre-treatment period, ice packs were …

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A Quantitative Study Of The Distinctive Proteins Of The Eosinophil: A Comparison Of Normals And Eosinophilic Patients, James M. Olson Dec 1984

A Quantitative Study Of The Distinctive Proteins Of The Eosinophil: A Comparison Of Normals And Eosinophilic Patients, James M. Olson

Honors Theses

Eosinophils have long been associated with parasitic and allergic diseases. Methods of procurement and purification of eosinophils in the last ten years have allowed intensive investigation into their components, properties, functions, and mechanisms (Gleich et al, 1982). The goals of this project are to separate eosinophils in blood samples taken from patients with eosinophilia and from normals based on density over distontinuous Metrizamide gradients; to determine quantitatively the levels of the three distinctive proteins of the eosinophil, specifically MBP, EDN, and ECP; and to compare the levels of these proteins found in eosinophils of patients with eosinophilia to the amounts …

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