Animal Experimentation and Research Commons^™

Metcalf-Hatch Repeal Means Lab Accountability, Henry Spira

Articles

No abstract provided.

Metcalf-Hatch Act: Profit, Politics And Pain, Henry Spira

Articles

No abstract provided.

Metcalf-Hatch Fact Sheet, Henry Spira

Metcalf-Hatch Campaign

The Metcalf-Hatch Act forces tax supported pounds and shelters to turn over unwanted cats and dogs to New York laboratories. This creates difficulties for shelters/pounds. People who are aware of Metcalf-Hatch (MH) will tend to abandon animals rather than risk having them recycled into labs. And this places additional tax burdens on communities for rounding up abandoned strays. The cost of stray animals is already $400 million a year in the USA.

Ultrastructural Study Of Wound Healing On A Root Planed And Citric Acid Treated Root Surface, Craig Martin Ririe

Loma Linda University Electronic Theses, Dissertations & Projects

It has been shown previously that a root surface which has been scaled, root planed and demineralized by topical application of citric acid in vitro, is dominated by exposed collagen fibrils. The purpose of this study was to examine the mechanism of healing and possible reattachment of soft periodontal connective tissue to the root planed and subsequently acid treated root surfaces and to determine whether new collagen fibrils produced in the connective tissue could attach to these exposed fibrils.

Full thickness tissue flaps were raised and the coronal 4-5 mm of the labial alveolar plate removed over the maxillary incisor …

Isolation And Chemico-Physiologic Characterization Of A Parotid Hormone Activity From Porcine Glands, Jean-Marc Tieche

Loma Linda University Electronic Theses, Dissertations & Projects

For the past decade, evidence has accumulated which suggests that the resistance of the teeth to dental decay is in part under systemic control. It was shown that the resistance is accomplished through the optimal operation of a dentinal fluid transport mechanism which is believed to be hormonally controlled by the hypothalamic-parotid gland endocrine axis. Although circumstantial evidence for such an endocrine function was strong, the isolation of a parotid hormone which stimulates the dentinal fluid transport mechanism remained to be accomplished.

Using modern biochemical techniques, a methodology was designed to isolate and purify the hormone from porcine parotid glands. …

Euthanasia Of Dogs And Cats: An Analysis Of Experience And Current Knowledge With Recommendations For Research, T. Carding, M. W. Fox

eBooks

1978 Special Report prepared by the Institute for the Study of Animal Problems

Influence Of Denervation And Hemithyroidectomy On The Age Related Increase In The Number Of Calcitonin-Containing Cells In Mouse Thyroid, Nualnoi Wechbanjong

Loma Linda University Electronic Theses, Dissertations & Projects

It has been concluded from this study that nerve fibers in the superior laryngeal nerves have an influence on the development of C-cells in the mouse thyroid. This conclusion is based on the results of two types of investigation. The first is an analysis of the effect of age on the number of C-cells and the second is an investigation of the influence of denervation and hemithyroid-parathyroidectomy on the age related changes in C-cells numbers.

I. The distribution and c011centraticn of C-cells in mouse thyroid at various ages.

Serial 6 µm sections of thyroid glands from Swjss Webster mice at …

Effects Of Trypanosoma Musculi Kendall, 1906 Immune Serum On The Reproduction Of Trichinella Spiralis (Owen, 1835) Railliet, 1896 In Mice, Samuel L. Chafin

Loma Linda University Electronic Theses, Dissertations & Projects

The possible effects of ablastin, a reproduction-inhibiting antibody which is produced during the infection of the blood parasite, Trypanosoma musculi in the mouse, were investigated by fractionating the parasite into a supernatant fraction (designated as Group A test mice) and a pellet fraction (Group B test mice). These groups A and B mice were injected with the respective fractions, plus Freund’s Complete Adjuvant (FCA). Control mice were injected with FCA only. Sera from these three groups of mice were removed after the Group A and Group B mice were shown to be negative to T. musculi challenge, and Group A …

Museum Victory For Animal Rights, Henry Spira

Commentaries and Editorials

No abstract provided.

Amnesty International Scandal, Henry Spira

Commentaries and Editorials

File also includes:

November 11, 1977

• Letter to the Editor from Michael E. Levin, Associate Professor of Philosophy, City University
• Spira response to Professor Levin
• Letter to the Editor from David Hawk, Executive Director, Amnesty International USA
• Spira response to David Hawk

February 23, 1978

• Letter from Mrs. F. Blakiston, Secretary to the International Executive Committee for Amnesty International to Henry Spira

March 1, 1978

• Memorandum from Leonard Rack M.D. and Henry Spira to the International Executive Committee for Amnesty International

July 25, 1978

• Letter from Dick Oosting, Deputy Secretary General for Amnesty International, to Henry Spira

October 1978

• Letter …

The Effects Of Inducing Functional Class Iii Malocclusion On Actively Growing Rat Mandibles, Aarin Chu

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Human Placental Alkaline Phosphatase And Steroid Metabolism By Rat Adrenals, Allen G. Meier

Loma Linda University Electronic Theses, Dissertations & Projects

Human placental alkaline phosphatase was purified by butanol extraction, methanol precipitation and chromatography on DEAE-cellulose. The pH optimum was found to be 10.9. The enzyme was competitively inhibited by di-ethyl-p-nitrobenzyl-phosphonate and copper sulfate. An increase in enzymatic activity occurred in the presence of the phosphate ion (HPO₄) and ATP.

The enzyme was crystallized from a 40% saturated solution of ammonium sulfate and shown to be homogeneous by sedimentation analysis. Homogeneity of the enzyme was also demonstrated using poly-acrylamide-gel-electrophoresis. At a pH of 7.5% the electrophoretic pattern yielded a single band and showed no significant contamination.

The metabolic transformation …

The Effect Of Endodontic Therapy On The Incidence Of Radiation Caries In Dogs, James A. Abbott

Loma Linda University Electronic Theses, Dissertations & Projects

Endodontic therapy was evaluated as a possible control or prevention modality of radiation type dental caries in dogs. Two adult male dogs with permanent dentitions had endodontic therapy performed on alternating teeth in each jaw. The nontreated teeth served as controls.

The dogs were irradiated with 7000 rad Cobalt-60 radiation fractionated over an eight week period. The dogs were killed at 60 and 182 days post-irradiation. The teeth were removed from the jaws and immersed in methylene blue dye, sectioned, and the dye penetration pattern photographed. Diffusion of the dye into the tooth structure was quantified and statistically analyzed.

A …

Apical Closure Induction Using Bone Growth Factors And Mineral Trioxide Aggregate, Kenneth W. Tittle

Loma Linda University Electronic Theses, Dissertations & Projects

This study compared the ability of three different bone growth factor combinations with that of Ca(OH)₂ to stimulate apical closure as well as the effectiveness of Mineral Trioxide Aggregate (MTA) as an apical barrier. Fifty-six roots of second and fourth mandibular premolars of seven six- month old beagle dogs were used. Periradicular lesions were created over a four week period. The canals were cleaned and shaped and filled with Ca(OH)₂ for one week. They were then irrigated and received one of seven different treatments in a balanced design. Growth factors were placed in a carboxymethylcellulose carrier and delivered …

Animal Rights: Nih Cat Sex Study Brings Grief To New York Museum, Nicholas Wade

Popular Press Items

No abstract provided.

Animals Suffer For Science, Henry Spira

Articles

No abstract provided.

Effects Of Cariogenic And Noncariogenic Diets On The Concentration Of The Parotid Hormone Releasing Factor In Rat Hypothalami, Melva Joan Brown

Loma Linda University Electronic Theses, Dissertations & Projects

Hypothalamic and cortical extracts from various cariogenically grouped rats which had been maintained on either a Purina diet or a high sucrose diet (HSD) for three months were infused intravenously into 28 days old rats to determine if there was a titer of parotid hormone releasing factor (PRF) capable of facilitating fluid movement (FM) in the teeth. The hypothalamic and cortical extracts obtained from Purina-fed rats with 0-caries and the hypothalamic extracts from BSD-fed rats conclusively demonstrated FM stimulatory ability. Other Purina-fed groups were essentially biologically ineffective, however, extracts from HSD-fed caries groups including scores 11-40 demonstrated extremely potent FM …

Histology Of Apical Tissue : A Comparative Study Of Sargenti Formula, Short Vs. Overfill, James G. Schulze

Loma Linda University Electronic Theses, Dissertations & Projects

Four Stump-tail monkeys (Macaca spiciosa) were used for this study to analyze, on a cellular level, the apical tissue of teeth overfilled versus teeth filled short 1-3 mm. of the radiographic apex with the Sargenti formula. In each animal four incisors were filled short and four incisors were overfilled. A final sample size consisted of 28 teeth. The time periods ranged from 48 hours to 120 days. After analysis of the histology it can be stated that the teeth filled short showed no inflammation to moderate inflammation in the periapical tissues. In teeth overfilled there was a range from moderate …

Determination Of Lead Content In Apical Tissue Following Root Canal Obturation With Sargenti's Formula, G. Merle Hickok

Loma Linda University Electronic Theses, Dissertations & Projects

Four Stump-tail monkeys were used to help determine if the lead in Sargenti's formula diffuses out into the apical tissue while confined entirely within the root canal. In each animal two teeth were filled with Sargenti's formula 1-2 millimeters short of the radiographic apex, and two teeth were filled with gutta percha and Proco-Sol sealer for controls, making a total sample size of sixteen. The time ranged from 48 hours to 120 days. Lead analysis using the spectrophotometric method showed a higher lead content in the teeth filled with Sargenti's formula than with the gutta percha controls.

The Effect Of Insulin, Fluoride And Methyl Xanthines On The Hypothalamic-Parotid Gland Endocrine Axis, Reagan Ondler

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to determine the effect of insulin and fluoride on dentinal fluid movement (FM) in high-sucrose fed rats as mediated through the hypothalamic-parotid gland endocrine axis. In conjunction with the fluoride study, the possible involvement of cAMP was examined through the use of several methyl xanthines which are recognized as phosphodiesterase inhibitors.

Twenty-one day old, male Sprague Dawley rats were maintained on a high-sugar diet (62% sucrose) for 8-9 days at which time intravenous or arterial infusions of insulin, fluoride or the methyl xanthines were performed. Additional procedures included collecting plasma from rats which had …

The Relationship Of Alcohol Consumption In Rats To Morphine And Codeine Intake And The Metabolism Of Dopamine, Hsiu Fen Liu

Loma Linda University Electronic Theses, Dissertations & Projects

The effects of morphine and codeine free-choice alcohol consumption in male Sprague-Dawley rats were studied. Intakes of a 10% alcohol solution in milliliters per 100 gm. of body weight were on recorded weekly. No difference was found in the alcohol intake of rats given either a morphine-supplemented diet (0.01% of the diet) or daily injection of morphine sulfate (2 mg./kg.); however, there was a significant lower intake of water (p < .05) in those rats fed the diet with morphine. Total fluid consumption of both groups given the morphine-supplemented diet and daily injection of morphine sulfate was decreased (p < .05). It was found that morphine did not affect the percentage of total fluid consumed as alcohol or the weight gain.

Two levels of codeine phosphate (10 mg./kg. and 20 mg./kg.) were injected subcutaneously into rats twice a day. It was observed that the codeine significantly decreased alcohol intake in rats …

Bone Regeneration Over Submerged Dental Roots In Dogs, Roger Lee Plata

Loma Linda University Electronic Theses, Dissertations & Projects

Nonendodontically treated roots were submerged 1.5 to 2.0 mm. below the alveolar crest in three dogs. Twelve roots were treated in this manner. Regeneration of alveolar bone can be seen in three weeks with complete bone coverage in five weeks. A complete lamina dura can be seen around the amputated roots.

Bone failed to regenerate over two of the twelve roots. In one case there was delayed healing due to the early loss of some sutures. A sinus tract developed connecting the oral environment with the contents of the pulp. This led to bone resorption and a necrotic pulp. In …

The Developmental Fine Structure Of Pacinian Corpuscles, Pedro B. Nava

Loma Linda University Electronic Theses, Dissertations & Projects

A preliminary light microscopic study of the mouse hind limb revealed a group of about 20 Pacinian corpuscles extending from the mid-region of the fibula to the tibio-fibular fusion strategically located for the possible sensing of bone-transmitted vibration.

The fine structure of the corpuscles is described in four stages: primordial or prenatal (19 and 20 days); early differentiation (1-8 days postnatal); differentiated corpuscle (9, 10, 12, 14 and 21 days postnatal); and adult (28 days old).

The corpuscles commence development during the 19th prenatal day. The corpuscle then consists of a nerve ending surrounded by sheath-cells which appear to be …

Histologic Comparison Of Apical Tissue Response To Endodontic Overfills With Gutta Percha And Grossman's Sealer Vs. Sargenti Method (N₂), Donald L. Brewer

Loma Linda University Electronic Theses, Dissertations & Projects

This study compared endodontic overfills of N₂ and gutta percha-Grossman's Sealer from a histopathological basis. The study employed four male rhesus monkeys and a sample of 31 teeth.

The most obvious difference between the samples filled with N₂ and the gutta percha-Grossman's Sealer samples was the recurring presence of ankylosis in the N₂-treated teeth. Results also included bone necrosis, inflammation and root and bone resorption.

The N₂ samples were considered to have evidenced greater violence of reaction apically.

Effects Of Cocaine, Nicotine And Diet On Voluntary Alcohol Consumption In Rats, Zaida Raquel Cordero Loayza

Loma Linda University Electronic Theses, Dissertations & Projects

The consumption of alcohol is a problem not only in this country (U.S.), but also in many other places in the world. Thus we find the Indians in Peru, Bolivia and other neighboring countries (Ecuador and northern part of Argentina) consuming alcohol to the point that they become alcoholics, in addition to this they also chew coca leaves, plant from which cocaine is extracted.

In an effort to establish the cause for this behavior, we put Sprague-Dawley albino rats on either a poor or good basal diet. Once every other day the rats were injected with a solution of cocaine. …

Viability Of Dentin In Rats Incisors : Fluid Movement In Pulpless, Endodontically Treated, And Normal Teeth In Rats, Maria Luisa Canales

Loma Linda University Electronic Theses, Dissertations & Projects

It is a well established fact that there is a fluid transport system through the dentin, however to the present time very little has been done regarding this system in teeth that have been endodontically treated.

The present work was conducted in vivo using the lower incisors of rats as the medium for the study.

A selected number of Sprague Dawley rats (36 animals), whose average age was 60 days, and whose body weight averaged about 180 grams, were given an anesthetic of Sodium Pentabarbitol, .05 to .07 ml. per 100 grams body weight. This anesthetic was injected intraperitoneally.

The …

The Effect Of Post-Operative Use Of Chlorhexidine On Periodontal Regeneration In Monkeys With Chronic Bifurcation Defects, J. Pat Bishop

Loma Linda University Electronic Theses, Dissertations & Projects

Periodontal regeneration and inflammation was evaluated histometrically in 36 paired teeth in six macque(sic) monkeys with chronic bifurcation defects. Daily topical TL Chlorhexidine was applied to one-half of the teeth, saline to the other half for six weeks after the bifurcation defects were surgically debrided. Measurements were made from a section taken from the mid-bifurcation for each tooth. The results demonstrated no significant difference in the area of inflammatory response or the degree of regenerative capacity of post-operative healing between Chlorhexidine treated and paired nontreated control teeth.

Effect Of Post-Operative Use Of Chlorhexidine On Regeneration Of Bifurcation Defects In Dogs, Gary Bogle

Loma Linda University Electronic Theses, Dissertations & Projects

The daily post-operative use of topical chlorhexidine application has been compared to daily toothbrushing. Periodontal defects were experimentally created in the bifurcations of lower premolars in dogs. The defects were treated by surgical debridement. Chlorhexidine application and toothbrushing were performed post-operatively for six weeks on contralateral pairs of teeth. The amounts of connective tissue and bone regeneration were evaluated from microscopic sections of the bifurcation areas. No difference between chlorhexidine and tooth brushing was found for the connective tissue attachment. However, significantly more bone had regenerated in the chlorhexidine treated areas than the tooth brushed area.

The Effects Of Crowding On The Diurnal Ascorbic Acid Levels Of Albino Rats, Mary Jo Barr

Masters Theses

The adrenal physiology of the albino rat was studied through the measurement of adrenal ascorbic acid levels. A cyclic phenomenon was observed which follows the diel periodicity of the animal. Ascorbic acid values were found to be characteristically higher in males than in females. A similar difference was also seen in populations divided into young animals (body weight less than 175 grams) and old animals (body weight greater than 175 grams). Crowding, induced by manipulating the area of cage space per gram of body weight, was observed to be a definite stressor and caused the adrenal ascorbic acid levels to …

The Effects Of Acute Stress On Adrenal Ascorbic Acid Levels, Robert Joseph Collier

Masters Theses

The diurnal rhythm of adrenal ascorbic acid (DAR) displays an inverse relationship to the diurnal rhythm of circulating levels of plasma corticosteroids. Alloxan monohydrate, 175 mg/kg body weight was given subcutaneously to albino rats to determine the effect of the diabetic state on the DAR. A total of 200 female albino rats were divided into two study groups. Study Group I was composed of 150 animals divided into populations of 15, each including 3 controls. Study Group II was composed of populations of 5, each group containing all controls or all alloxinated rats. The populations of 15 were sacrificed at …