Animal Experimentation and Research Commons^™

Sprague-Dawley Rats Fed Milk Or Beef Protein : Differences In Response To 1,2-Dimethylhydrazine Carcinogenesis, Stanley W. L. Ng

Loma Linda University Electronic Theses, Dissertations & Projects

Colon cancer is responsible for a high percentage of cancer deaths in developing countries, and there is convincing epidemiological evidence that meat protein and fat in the diet increase the incidence of this form of cancer. An animal tumor model which has been used by many investigators interested in colon cancer is the 1,2-dimethylhydrazine (DMH)-rat or DMH-mouse tumor model. In previous work done in this laboratory the DMH-BALB/c mouse tumor model was used to compare the production of colon tumors in mice fed a diet using non-fat powdered milk as a source of protein with those in mice fed beef …

Stability In Mechanical Expansion Of The Maxilla With Surgical/Mechanical Expansion Of The Mandible In An Adult Baboon, Robert D. Mitchell

Loma Linda University Electronic Theses, Dissertations & Projects

Expansion of the maxilla at the midpalatal suture has had much investigation and is a recognized option in orthodontic therapy. Lateral expansions of the mandible through surgical separation at the symphysis have been done in animal studies. A combination of these procedures has not yet been done together.

The purpose of this study was to expand both the maxilla and the mandible of a baboon simultaneously while maintaining occlusion. The amount of expansion and stability was studied using photographs, models, cephalograms, and tetracycline bone labels using U.V. microscopy.

A young adult baboon with full dentition and normal occlusion was used. …

Age-Related Changes Of Neurites In Meissner Corpuscles Of Diabetic, Vicky R. Ras

Loma Linda University Electronic Theses, Dissertations & Projects

This thesis is based on a qualitative and quantitative study of the neural component of Meissner corpuscles of diabetic and non-diabetic mice. Age-related changes were also documented in these mechanoreceptors. Corpuscles from forty-six diabetic mice and forty-six non-diabetic mice ranging from 2.5 to 17 months old were studied. Forepaw index digital pads were formalin-fixed and frozen-sectioned parallel to each digit and perpendicular to the skin. Serial sections were then silver-impregnated to allow light microscopic examination of the neurites in the corpuscles.

Neurites became more coarse, tortuous, branched, varicose, and thus more complex until middle age. Corpuscle size appeared greatest at …

Observation Of Bone Formation In The Mandibular Symphysis Following Surgical Separation And Slow Mechanical Expansion In A Baboon, Robert J. Homer

Loma Linda University Electronic Theses, Dissertations & Projects

Mechanical expansion of the maxilla, at the midline suture, is a widely accepted orthodontic procedure used to gain arch width in certain types of maxillary deficient arches.

In animal research on the mandible, techniques using surgical separation at the symphysis with immediate expansion up to 12 mm, fixation with a metal splint, and autogenous bone transplanted into the expanded area, have been reported to be successful.

To date, no experiment utilizing slow mechanical expansion of the mandible at the symphysis has been reported.

In this pilot study, the mandible of a female baboon was surgically separated A-P at the symphysis. …

The Effect Of Escherichia Coli Endotoxin On The Cat Periapical Tissues, Thomas G. Dwyer

Loma Linda University Electronic Theses, Dissertations & Projects

The periapical tissue reaction to three concentrations of endotoxin solutions and to a sterile saline control solution was examined in six adult cats. The maxillary and mandibular cuspids were isolated and the pulps extirpated with barbed broaches. The four solutions (1, 10, 100 μg/ml endotoxin, and saline) were then injected into the four prepared teeth for each cat and the access cavities sealed with IRM.

At two week intervals, two cats were sacrificed and the specimens removed, while the remaining animals were retreated as previously described. The block tissue sections were radiographed, fixed in 10% buffered formalin, and prepared for …

Cell Mediated Immunity By Cytotoxicity Assay And The Effect Of Corynebacterium Parvum And Radiation On Mice Bearing Herpes-Induced Tumors, Robert B. Stagg

Loma Linda University Electronic Theses, Dissertations & Projects

Cell mediated immune (CMI) cytotoxic reactivity of Balb/c mice against H238 cells, a Herpes simplex virus (HSV) Type 2 - induced sarcoma, was measured by the ¹²⁵IUdR release assay. The Balb/c mouse response to the growing HSV-induced tumor, treated with radiation and , was measured by survival rate, tumor growth and immune cytotoxicity of spleen and peritoneal exudate cells (PEC) as determined by ¹²⁵IUdR release assay. Subcutaneous (s.c.) inoculation of 1 x 10⁶ H238 cells (high dose) produced progressive tumor growth while s.c. inoculation of 1 x 10⁴ H238 cells (low dose) produced no tumors. A …

The Effect Of Endodontic Therapy On The Incidence Of Radiation Caries In Dogs, James A. Abbott

Loma Linda University Electronic Theses, Dissertations & Projects

Endodontic therapy was evaluated as a possible control or prevention modality of radiation type dental caries in dogs. Two adult male dogs with permanent dentitions had endodontic therapy performed on alternating teeth in each jaw. The nontreated teeth served as controls.

The dogs were irradiated with 7000 rad Cobalt-60 radiation fractionated over an eight week period. The dogs were killed at 60 and 182 days post-irradiation. The teeth were removed from the jaws and immersed in methylene blue dye, sectioned, and the dye penetration pattern photographed. Diffusion of the dye into the tooth structure was quantified and statistically analyzed.

A …

Apical Closure Induction Using Bone Growth Factors And Mineral Trioxide Aggregate, Kenneth W. Tittle

Loma Linda University Electronic Theses, Dissertations & Projects

This study compared the ability of three different bone growth factor combinations with that of Ca(OH)₂ to stimulate apical closure as well as the effectiveness of Mineral Trioxide Aggregate (MTA) as an apical barrier. Fifty-six roots of second and fourth mandibular premolars of seven six- month old beagle dogs were used. Periradicular lesions were created over a four week period. The canals were cleaned and shaped and filled with Ca(OH)₂ for one week. They were then irrigated and received one of seven different treatments in a balanced design. Growth factors were placed in a carboxymethylcellulose carrier and delivered …

The Effect Of Elevated Glucose On Neutrophilic Phagocytosis In Genetically Diabetic Mice, Larry B. Thompson

Loma Linda University Electronic Theses, Dissertations & Projects

Infiltration to an injury site or area of bacterial contamination by polymorphonuclear leucocytes (or neutrophils) is an early and essential phase of the early inflammatory reaction. Their action on foreign matter is the most critical step taken by body defense mechanisms in the first 24 hours. Anything which interferes with that action, phagocytosis, greatly hampers early inflammation. This study was designed to see the effect of elevated glucose levels on neutrophil phagocytosis in a unique strain of genetically diabetic mice.

Twenty-two diabetic and twenty-four control female mice were used. Blood glucose determinations and total white counts were made on each …

Some Aspects Of Cranial Bone Growth In The Puberal Macaca Mulatta Monkey: As Evidenced By Metallic Implants And Cephalometric Roentgenography And The Application Of A Geological Lapidary Technique For The Semi-Serial Thin Sectioning Of Undecalcified Bone, Lee Eugene Olsen

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to investigate cranial and facial bone growth in the puberal Macaca mulatta monkey over a six month period utilizing the methods of metallic implantation combined with serial cephalometric roentgenography and a bichromatic vital staining method.

The employment of the vital stains, alizarin Red S and anthracene blue, over the six month period of the study, failed to stain the actively growing bone as evidenced by gross and microscopic analysis of the undecalcified bone preparations.

Interimplant measurements were made across certain bone sutures and the following results were obtained: The zygomaticotemporal sutures showed marked growth …

A Cephalometric, Anatomic And Histologic Investigation Of The Effects Resulting From A Heavy Interrupted Orthopedic Force To The Maxilla Of The Macaca Mulatta Monkey, Roger Wayne Triftshauser

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to determine changes that occur in the craniofacial complex, when a heavy interrupted orthopedic force is employed to retract the maxilla in the post puberal rhesus monkey. Cephalometric, histologic and anatomic examinations were utilized to substantiate the findings.

Four female rhesus monkeys were used for experimentation. Two were experimental animals and the other two controls. Cervical retraction with headgear was applied over a period of 313 days and 159 days to the two experimental animals. Forces not exceeding 2.5 lbs. were found most effective.

The findings were as follows:-

1. A total posterior displacement of …

Craniofacial Changes Resulting From Expansion Of The Midpalatal Suture In The Macaca Mulatta Monkey As Revealed By Roentgenographic Analysis, Raymond M. Sugiyama

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to determine the effects of midpalatal suture separation on the craniofacial complex by quantitative roentgenographic analysis.

Ten female Macaca mulatta monkeys were randomly grouped for experimentation. Six animals were paired in groups for two months of treatment, four months of treatment followed by two months of post retention, and six months of treatment. The four remaining monkeys were paired and served as control animals for two and six month periods. Total activation of the expansion appliances in the treated animals was completed over a three week period.

The findings of this study were based …

Pharmacological Studies Of Antidiabetic Chinese Herbs, Mun Fa Lee

Loma Linda University Electronic Theses, Dissertations & Projects

Rehmannia glutinosa, Atractylus ovata, and Polygonatum chinense have all been employed by the Chinese as oral antidiabetic agents and reported by Min (1928), Kin (1931), and Li et al. (1936) to possess hypoglycemic activity. This prompted the pharmacological studies of these herbs.

Materials and methods. Their pharmacognosy was condensed from all available Chinese and scientific literature. Preliminary chemical and physical analyses included determinations for moisture, ash, crude fat, carbohydrates, protein, phosphorus, pH, alkaloids, melting point, and spectroscopy. Employing the Somogyi-Nelson method, their glycemic activity was studied in rats, rabbits, and dogs. Stress reactions were minimized. The effect …

The Effect Of Adrenal Steroid Hormones On The Protein Content Of Tumorous Livers, Duncan W. Martin

Biology ETDs

Protein is basic to the structure and function of protoplasm. Enzyme systems which regulate the rate of cellular reactions are primarily composed of protein and many of these enzymatic systems influence the metabolism of less specialized proteins that form the major structural material of protoplasm. It is believed that experimental procedures which modify protein composition of cells may also influence the activity of intra-cellular enzymes. In experimental live cancer, it has been shown that the activities of a number of oxidative enzymes are modified as the carcinogenic process proceeds (Richmond, 1955, p.38). Furthermore, it has been demonstrated that endocrine function …

Kidney Of The Frog, Felix Taubman

Bachelors’ Theses

The frog has been used exclusively in both high school and college as the main source of experimental biological and zoological studies. Because it is so easily obtained and so finely adapted to our purposes, we chose it as the specimen upon which to do our thesis work. Much has been done experimentally on the kidneys with the frog. The purpose of this thesis is to present a general view of the external and microscopic anatomy of the frog kidney and with this as a groundwork to present the problems of secretion; whether secretion is carried on by the renal …