Animal Experimentation and Research Commons^™

The Effect Of Guidor™ And Hop-1 On Hard Tissue Healing : Following Periradicular Surgery In Cats, Hugh Maguire

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to evaluate the effects of the resorbable membrane Guidor™ and the osteogenic protein hOP-1 on hard tissue healing following periradicular surgery in cats.

Surgical endodontic therapy was performed on the maxillary canine teeth of twelve cats. After orthograde root canal therapy, flap reflection, osteotomy, resection of the root end, root end cavity preparation and the placement of a root end filling, eight of the surgical sites were treated with Guidor™ and eight with hOP-1. The remaining eight sites received no further treatment and served as controls.

The animals were sacrificed at twelve weeks following …

Bone Morphogenetic Proteins And Periodontal Repair, Michael B. Lee

Loma Linda University Electronic Theses, Dissertations & Projects

For over thirty years now, research has been carried out to isolate and purify bone morphogenetic protein (BMP), a substance which has been shown to induce heterotopic bone formation in various animal species. Recent advances in the fields of developmental biology, molecular biology and genetics, have shown that the BMPs not only are responsible for postfetal bone induction (including normal bone remodeling, healing and repair), but are also critical during embryogenesis - not only in regards to the skeletal system, but quite possibly in the morphogenesis and pattern formation of other tissues and organs as well.

Due to their bone …

Alveolar Bone And Cementum Regeneration Following Periodontal Reconstructive Therapy : A Review Of Histologic Observations In Man And Experimental Animals, Karen E. Rodden

Loma Linda University Electronic Theses, Dissertations & Projects

Periodontal regeneration includes the formation of both alveolar bone and cementum. Studies reporting histologic evidence of healing following periodontal reconstructive therapy were reviewed to determine whether regeneration of these structures improves with increasing healing interval, and whether they regenerate independently or whether regeneration of one structure is dependent upon regeneration of the other. Additionally, the review addresses whether specific reconstructive modalities predictably yield increased amounts of regeneration. Differences in regenerative potential between humans, nonhuman primates, and canines were also examined. Results of over a decade of studies of periodontal reconstructive therapy were tabulated by family (human, nonhuman primate, and canine) …

Periodontal Repair In Dogs : Evaluation Of Rhbmp-2 Carriers, Lauralee Nygaard

Loma Linda University Electronic Theses, Dissertations & Projects

This study evaluated candidate carriers for recombinant human bone morphogenic protein-2 (rhBMP-2) in periodontal reconstructive surgery.

Canine demineralized bone matrix (DBM), bovine inorganic crystallene bone matrix (Bio-Oss), bovine derived microfibrillar collagen matrix (Helistat), poly (D,L-lactide-coglycolide) micropaiticles (PLGA), and polylactic acid granules (Drilac) were used with rhBMP-2 (20 (µg/100 µl implant volume) in routine critical size canine supraalveolar periodontal defects. Contralateral defects in six beagle dogs were randomly assigned to receive: DBM/rhBMP-2, DBM-control, Bio-Oss/rhBMP-2, Helistat/rhBMP-2, PLGA/rhBMP-2, or Drilac/rhBMP-2, all with autologous blood except for Helistat and PLGA. Animals were sacrificed eight weeks post-surgery and block sections of the defects were processed …

Experimental Peri-Implant Tissue Breakdown In Rhesus Monkeys, Oliver Hanisch

Loma Linda University Electronic Theses, Dissertations & Projects

The aim of this investigation was to characterize peri-implant tissue breakdown following ligature-induced plaque accumulation around osseointegrated implants in the rhesus monkey. Two hydroxyapatite-coated cylindrical implants were inserted bilaterally in the second premolar and first molar region of both mandible and maxilla in four non-human primates (Macaca mulatto). The implants were left submerged to osseointegrate for one year. Eight weeks after healing abutment connection, a two-month plaque control program was initiated. At the end of this period, clinical and radiographic recordings were carried out, and peri-implant subgingival microbial samples were obtained (baseline). Subsequently, cotton ligatures were placed around …

Healing Potential Of Osteotomies Of The Nasal Sinus In The Dog, Roderick W. Tataryn

Loma Linda University Electronic Theses, Dissertations & Projects

Maxillary sinus osteotomy is sometimes indicated for transantral surgical approaches to palatal roots of maxillary molars. A search of the literature showed an absence of any report on the healing potential of such defects. The purpose of this study was to determine histologically the reparative process of the nasal sinus of dogs following small and large surgical antral perforations. After anesthetizing six beagle dogs, full thickness periosteal flaps were raised overlying the maxillary buccal cortical plates. A 5x5 mm diameter perforation was created with a trephine bur above the second maxillary premolar on one side of each animal. On the …

Measurement Of Blood Flow In Reflected Muco-Gingival Tissue Flaps In Cats : Using The Radiolabeled Microsphere Method, Gary T. Wuchenrich

Loma Linda University Electronic Theses, Dissertations & Projects

Blood flow to the maxilla and mandible may vary due to differences in vascular supply and in physiologic, metabolic and pathologic conditions. In general it is accepted that oral tissues have an abundant blood supply; however, there is little information quantifying blood flow to these regions. In this study, the maxillary tissues were chosen to measure the blood flow in attached and reflected gingival tissue in adult cats using the radiolabeled microsphere method.

Sixteen cats with permanent dentition, clean mouths, and without inflamed gingiva upon visual inspection were used and divided into two groups. Group 1 consisted of six cats …

Periodontal Repair In Dogs : Part I : Evaluation Of The Natural Disease Model ; Part Ii : Eptfe Barrier Membranes Support Wound Stabilization And Enhance Bone Regeneration, James Marc Haney

Loma Linda University Electronic Theses, Dissertations & Projects

Animal models are frequently consulted for histometric analysis of reconstructive periodontal therapy. Statistical analysis of induced lesion defects and surgically created defects from our laboratory suggest defect size homogeneity is critical for the model to discriminate treatment effects. This study characterizes natural disease defects in beagle dogs. Buccal-lingual histologic sections from the 2nd, 3rd, and 4th mandibular premolar teeth (P2, P3, P4) from 6 aged beagle dogs with natural disease defects were evaluated for variation in defect height between pair matched tooth types, and jaw quadrants, as well as estimated confidence intervals for treatment differences between left and right pair …

A Non-Human Primate Model For Evaluating A Fenestration Corticotomy Technique, Philip G. M. Khng

Loma Linda University Electronic Theses, Dissertations & Projects

A study model has been designed to evaluate the effectiveness of a new fenestration corticotomy procedure in two non-human primates Macaca fasicularis.

The objective of this study was to evaluate the efficacy of a new conservative corticotomy procedure to facilitate dental translation using fenestrations. This pilot study compared treatment and control quadrants of each individual animal's maxilla and mandible with both having the same retraction appliance and force on the 2nd premolar following extraction of the 1st molars. The null hypothesis stated that there was no significant statistical difference between the new conservative corticotomy procedure (fenestrations) when compared with …

Development Of Periapical Lesions In Immunosuppressed Rats, Phillip A. Waterman Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

The pathogenesis of periapical lesions is the interaction of the oral flora with the existing host defenses. The role of bacteria has been established in endodontic disease but the contribution of the host defenses in periapical lesion formation is less clear. The purpose of this study was to compare periapical lesion development in immunosuppressed rats with that in normal rats. Thirty-five, eight-week old Sprague-Dawley rats were equally divided into seven groups. Groups 1, 2, and 3 were given weekly injections of Cytoxan, an immunosuppressive agent. Groups 4, 5, and 6 received no immunosuppressive therapy. Group 7 served as a control, …

Transforming Growth Factor-Beta 1 Expression In Developing Ovine Teeth As Visualized By In Situ Hybridization, Søren Jepsen

Loma Linda University Electronic Theses, Dissertations & Projects

Recently we demonstrated that human dentin contained relatively high amounts of transforming growth factor beta (TGF-ß) and proposed that dentin TGF-ß originated from odontoblasts. Because of the suggested role of TGF-ß in mediating cellular interactions in dental cell development by paracrine mechanisms, we studied the expression of TGF-ß message in developing teeth by in situ hybridization. 5 µm serial sections of neonatal sheep mandibular third molars that had been fixed in glutaraldehyde and paraffin-embedded were used for in situ analysis. An [³⁵S]-labeled cRNA probe, complementary to TGF-ß1 mRNA, was constructed from human TGF-ß1 cDNA. Northern analysis of total …

New In Vivo And In Vitro Techniques For Quantification Of Lateral Force Levels That Cause Implants To Fail : A Progress Report On Monkey Research, V. Leroy Leggitt

Loma Linda University Electronic Theses, Dissertations & Projects

Two new techniques for quantification of forces generated by an expansion screw apparatus placed between pairs of cylindrical endosseous titanium implants are described, along with preliminary data gained from their use in Macaca rhesus monkeys. Strain gages were bonded to the expansion apparatus and were calibrated either directly by micro load cell, or indirectly by Instron Machine to reflect the applied force.

Immediate loads of up to 6 kilograms have been applied to the implants with no increase in mobility as verified by a Periotest device. No previous studies on the orthodontic uses of implants have evaluated in vivo forces …

The Role Of T Cells In The Pathogenesis Of Periapical Lesions, John B. Wallstrom

Loma Linda University Electronic Theses, Dissertations & Projects

The pulps of mandibular molars of 15 athymic and 15 conventional rats were surgically exposed and left open to their oral flora. Each group was divided into 3 subgroups of 5 animals each. The rats were sacrificed after their pulps were exposed for 2, 4, or 8 weeks. After fixing, decalcifying, and embedding, the specimens were sectioned and stained with H and E. They were then examined under a microscopic grid and quantified by percentages of surface areas of bone, connective tissue, bone marrow, intrabony spaces, periapical lesions, and numbers of osteoclasts. This was done using a DataVoice computerized data …

Histomorphometric Analysis Of Primate Periodontal Tissues Following Corticotomy In Orthodontics, Wuiteng Koh

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to evaluate the prevalence of specific cell types and structures at the active tooth movement site in corticotomized and non-corticotomized specimens obtained from a previous study, and to consider the hypothesis that corticotomy facilitated orthodontics is efficacious due to an inflammatory response rather than tooth-bone block movement. Specimens from 3 monkeys, each of which had a treatment and control quadrant in each arch, were sectioned, mounted on glass slides, and stained with Giemsa, haematoxylin, and Safranin stains. A histomorphometric analysis was performed with DataVoice™, a voice activated computer system which allowed for digitization of …

The Effect Of An Anti-Osteoclastic Agent And A Collagen Sponge On Root Resorption Following Regenerative Periodontal Surgery In The Dog, Rudolf Hahn

Loma Linda University Electronic Theses, Dissertations & Projects

Previous studies indicate that root resorption is a frequent sequela to significant amounts of periodontal repair following experimental regenerative periodontal surgical procedures in animal models. To study if root resorption can be reduced or prevented, periodontal repair was evaluated in large horizontal circumferential defects in the dog in the absence of influence from the oral environment. Diphosphonate (Cl₂ MDP) applied topically to the root surfaces prior to wound closure as well as the use of a collagen sponge membrane (Helistat®) draped over the root surfaces and a control with no additional treatment after defect creation were the modalities employed. …

Electromagnetic Field Variations In The Activation Of Bone Cell Synthesis, Gary Ketcherside

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

A Non-Human Primate Model For Studying Corticotomy-Facilitated Orthodontic Tooth Movement, R. David Rynearson

Loma Linda University Electronic Theses, Dissertations & Projects

This study was undertaken to test the hypothesis that a corticotomised tooth moves as a tooth-bone unit when under orthodontic force. A non-human primate model was designed to test the hypothesis. The non-human primate selected was Macaca fasicularis. Three adult males with erupted third molars were chosen so that extrapolation could be made to the adult human. The methods employed included the following: 1) Each animal had one treatment quadrant and one control quadrant per arch; 2) Pre-op and post-op study models were made; 3) All 2nd premolars, 2nd molars, and 3rd molars were banded; 4) A full-thickness flap …

Histologic Host Response To Polyoxymethylene Prosthesis As A Temporomandibular Joint Condylar Replacement, Frederick R. Mathews

Loma Linda University Electronic Theses, Dissertations & Projects

Polyoxyinethylene (Delrin) is a polyacetyl homopolymer which has been used successfully in many orthopedic joint replacements. To determine histologic host response to Delrin as a temporomandibular joint condylar replacement condyles were removed from ten fully mature macaca fasicularis monkeys. The condyles were replaced with a total prosthetic Delrin condyle attached to a titanium mesh. Specimens obtained at one, two, and six months indicated total acceptance of the Delrin, remodeling of the glenoid fossa and formation of bone between the middle cranial fossa and the roof of the glenoid fossa. These findings would indicate Delrin is a viable option for condylar …

The Use Of Dura Mater As A Meniscus Replacement In Tmj Surgery, Kim J. Dipasquale

Loma Linda University Electronic Theses, Dissertations & Projects

Internal derangement of the temporomandibular joint is recognized as a problem of significant magnitude in both medical and dental circles. The last decade has seen a steady progression in the knowledge and sophistication of not only the anatomy and physiology of the temporomandibular joint, but also in the techniques of non-surgical and surgical management of internal derangements. Surgical correction of the unrepairable disc has typically been addressed by performing a menisectomy followed by placement of a non-physiologic implant material.

The animal study reported here was performed in order to evaluate the histologic fate and biological compatibility of freeze-dried dura when …

Effects Of Lateral Mandibular Expansion On Mandibular Morphology In The Growing Rat : A Comparative Study Of Treatment Effects With Orthopaedic And/Or Surgical Procedures, Eric Looi

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to investigate the effects of mandibular expansion by orthopaedic and surgical procedures in the growing rat mandible.

Sixty-four 35 day old Sprague Dawley Harlan rats were divided into four groups of 16 rats each and categorized as the control, orthopaedic expansion, surgery without graft and surgery with graft respectively. Prefabricated appliances were used for orthopaedic expansion of the mandibles and maintaining surgical expansion at the symphysis in the respective groups. Oxytetracycline were given intraperitoneally to mark areas of bone remodeling. Half the number of rats from each group were sacrificed at the end of …

Stability In Mechanical Expansion Of The Maxilla With Surgical/Mechanical Expansion Of The Mandible In An Adult Baboon, Robert D. Mitchell

Loma Linda University Electronic Theses, Dissertations & Projects

Expansion of the maxilla at the midpalatal suture has had much investigation and is a recognized option in orthodontic therapy. Lateral expansions of the mandible through surgical separation at the symphysis have been done in animal studies. A combination of these procedures has not yet been done together.

The purpose of this study was to expand both the maxilla and the mandible of a baboon simultaneously while maintaining occlusion. The amount of expansion and stability was studied using photographs, models, cephalograms, and tetracycline bone labels using U.V. microscopy.

A young adult baboon with full dentition and normal occlusion was used. …

Observation Of Bone Formation In The Mandibular Symphysis Following Surgical Separation And Slow Mechanical Expansion In A Baboon, Robert J. Homer

Loma Linda University Electronic Theses, Dissertations & Projects

Mechanical expansion of the maxilla, at the midline suture, is a widely accepted orthodontic procedure used to gain arch width in certain types of maxillary deficient arches.

In animal research on the mandible, techniques using surgical separation at the symphysis with immediate expansion up to 12 mm, fixation with a metal splint, and autogenous bone transplanted into the expanded area, have been reported to be successful.

To date, no experiment utilizing slow mechanical expansion of the mandible at the symphysis has been reported.

In this pilot study, the mandible of a female baboon was surgically separated A-P at the symphysis. …

The Effects Of Orthodontic Repositioning Into A Surgically Expanded Site On Bone Formation And Tooth Mobility In A Baboon, Anthony B. Lier

Loma Linda University Electronic Theses, Dissertations & Projects

For sometime, expansion of the maxillary arch, by means of surgical and mechanical procedures, have been acknowledged with minimal attention directed toward the mandible. In this pilot study a surgical technique was employed in separating the mandible, A-P, at the symphysis. The interosseous void, approximately 10 mm., was then filled with autogenous bone marrow collected from the greater trochanter and the crest of the ilium.

The surgical site was then maintained for a period of eight (8) weeks with the aid of a precast chromium-cobalt splint and titanium mesh placed intradentally and at the symphysis, respectively. Following the stabilization period …

Orthognathic Surgical Expansion Procedure Of The Mandible Of Female Baboon Papio Sp?, Willis L. Schlenker

Loma Linda University Electronic Theses, Dissertations & Projects

Expansion of the maxillae is a well recognized procedure in orthodontics. Little is known about Mandibular Expansion. This project is a pure research of the possibility of expanding the mandible laterally at the symphysis on a baboon.

The symphysis was cut A-P and expanded laterally 11 mm, then held and stabilized with a titanium mesh work screwed to the inferior border of the mandible. The defect area was filled with a bone graft of marrow and cancellous bone fragments taken from the greater trochanter and the crest of the ilium. New bone did fill in the defect and the expansion …

An Ultra-Violet Light Microscopic Study Of The Hydroxylapatite Ceramic-Bone Interface Phenomena, Leonard W. Pierce

Loma Linda University Electronic Theses, Dissertations & Projects

Preparations used in this study were obtained from an investigation which had undertaken the following:

Durapatite, a hydroxylapatite ceramic was implanted in 24 mature Beagle dogs to study its maintenance effect on the alveolar bone of the mandible.

After dividing the dogs into two groups, a fresh socket and a healed socket group, the posterior teeth were removed and alveolectomies were performed. Eight ceramic implants were then placed in the right mandible of each animal in each group. The healed group received the implants five weeks after tooth removal and the fresh socket group received implants immediately after extraction.

The …

The Effect Of Escherichia Coli Endotoxin On The Cat Periapical Tissues, Thomas G. Dwyer

Loma Linda University Electronic Theses, Dissertations & Projects

The periapical tissue reaction to three concentrations of endotoxin solutions and to a sterile saline control solution was examined in six adult cats. The maxillary and mandibular cuspids were isolated and the pulps extirpated with barbed broaches. The four solutions (1, 10, 100 μg/ml endotoxin, and saline) were then injected into the four prepared teeth for each cat and the access cavities sealed with IRM.

At two week intervals, two cats were sacrificed and the specimens removed, while the remaining animals were retreated as previously described. The block tissue sections were radiographed, fixed in 10% buffered formalin, and prepared for …

Ultrastructural Study Of Wound Healing On A Root Planed And Citric Acid Treated Root Surface, Craig Martin Ririe

Loma Linda University Electronic Theses, Dissertations & Projects

It has been shown previously that a root surface which has been scaled, root planed and demineralized by topical application of citric acid in vitro, is dominated by exposed collagen fibrils. The purpose of this study was to examine the mechanism of healing and possible reattachment of soft periodontal connective tissue to the root planed and subsequently acid treated root surfaces and to determine whether new collagen fibrils produced in the connective tissue could attach to these exposed fibrils.

Full thickness tissue flaps were raised and the coronal 4-5 mm of the labial alveolar plate removed over the maxillary incisor …

Isolation And Chemico-Physiologic Characterization Of A Parotid Hormone Activity From Porcine Glands, Jean-Marc Tieche

Loma Linda University Electronic Theses, Dissertations & Projects

For the past decade, evidence has accumulated which suggests that the resistance of the teeth to dental decay is in part under systemic control. It was shown that the resistance is accomplished through the optimal operation of a dentinal fluid transport mechanism which is believed to be hormonally controlled by the hypothalamic-parotid gland endocrine axis. Although circumstantial evidence for such an endocrine function was strong, the isolation of a parotid hormone which stimulates the dentinal fluid transport mechanism remained to be accomplished.

Using modern biochemical techniques, a methodology was designed to isolate and purify the hormone from porcine parotid glands. …

The Effects Of Inducing Functional Class Iii Malocclusion On Actively Growing Rat Mandibles, Aarin Chu

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

The Effect Of Endodontic Therapy On The Incidence Of Radiation Caries In Dogs, James A. Abbott

Loma Linda University Electronic Theses, Dissertations & Projects

Endodontic therapy was evaluated as a possible control or prevention modality of radiation type dental caries in dogs. Two adult male dogs with permanent dentitions had endodontic therapy performed on alternating teeth in each jaw. The nontreated teeth served as controls.

The dogs were irradiated with 7000 rad Cobalt-60 radiation fractionated over an eight week period. The dogs were killed at 60 and 182 days post-irradiation. The teeth were removed from the jaws and immersed in methylene blue dye, sectioned, and the dye penetration pattern photographed. Diffusion of the dye into the tooth structure was quantified and statistically analyzed.

A …