Research Methods in Life Sciences Commons^™

Nectar Source Preferences Of The Hummingbird: A Field Study From South Texas, Jasmine Benavidez

Honors Theses

With the rise of urbanization, comes a rise in urban-like activities—most notable is birdfeeding. Given the increasing popularity of bird-feeding, especially in hummingbird feeding, concern for implications, if any, has mounted. There is reason to believe that artificial feeders can impact the surrounding environment; that is, it is possible that feeders could attract pollinators away from flowering plants, which, in turn, could bring repercussions for pollination and all associated with it. However, literature, on the matter, is rather limited. To add to this, past studies provide two opposing arguments. This study aims to explore the nectar source preferences of hummingbirds …

Good Cop/Bad Cop: A Comparative Analysis Of Affiliative And Aggressive Behaviors By Adult Female And Male Olive Baboons With Infants At Randilen Wildlife Management Area, Tanzania, Jesse Elop

Independent Study Project (ISP) Collection

This study investigated the infant-directed behaviors of adult male and female olive baboons in Randilen Wildlife Management Area in April of 2022. The objectives were 1) To determine the difference between olive baboon males and females in frequency and duration of listed behaviors towards infants; 2) To classify the interactions of adult male and adult female olive baboons with infants as either primarily affiliative or primarily aggressive; 3) To determine the frequency of selected behaviors at different reproductive phases (lactation, estrus, neutral, indeterminant) amongst female olive baboons towards infants. A single baboon troop in Randilen Wildlife Management Area was observed …

A Quantitative Study Of Orchids And Their Proximate Environments Over An Elevational Gradient On The Northern Slope Of Montagne D’Ambre, Hazel Schrader

Independent Study Project (ISP) Collection

This research investigated change in orchid populations and their proximate environments on the Northern slope of Montagne d’Ambre, a subhumid forest in Northern Madagascar. Orchid density, way of life (epiphyte or terrestrial), phenology, and associations were recorded over 100m elevation gradients and between forest types (primary, secondary, dry, and humid forests at the same altitude). Additionally, orchid preferences for certain heights in the forest and DBH of host trees were analyzed. The establishment of 50X20m plots in 100m elevation gradients combined with ground survey yielded significant results demonstrating change along with elevation as well as forest types. Orchid density increased …

Behavioral And Physiological Effects Of Heavy Metals On Fish: A Review And Preliminary Results, Diana V. Morales

Theses and Dissertations

Pollution, specifically heavy metal pollution, in various bodies of water has been a significant issue for decades. Research has been conducted on heavy metals and their effects on the environment, larger fish species, and humans since the 1970s (Skidmore, 1964; Adeniyi et al., 2007; Adeosun et al., 2015; Bawuro et al., 2018). It is known that zinc is a neurotoxin in some species and causes harm to the environment. It is also known that it causes harm to people if it is ingested or if they are exposed to it for long periods of time (Mahurpawar, 2015; Mizuno and Kawahara, …

Soy Formula Is Not Estrogenic And Does Not Result In Reproductive Toxicity In Male Piglets: Results From A Controlled Feeding Study, Martin J.J. Ronis, Horacio Gomez‐Acevedo, Kartik Shankar, Leah Hennings, Neha Sharma, Michael L. Blackburn, Isabelle Miousse, Harry Dawson, Celine Chen, Kelly E. Mercer, Thomas M. Badger

School of Graduate Studies Faculty Publications

Soy infant formula which is fed to over half a million infants per year contains isoflavones such as genistein, which have been shown to be estrogenic at high concentrations. The developing testis is sensitive to estrogens, raising concern that the use of soy formulas may result in male reproductive toxicity. In the current study, male White‐Dutch Landrace piglets received either sow milk (Sow), or were provided milk formula (Milk), soy formula (Soy), milk formula supplemented with 17‐beta‐estradiol (2 mg/kg/d) (M + E2) or supplemented with genistein (84 mg/L of diet; (M + G) from postnatal day 2 until day 21. …

Concentrations Of Digestible, Metabolizable, And Net Energy In Coconut Co-Products Fed To Growing Pigs, Ronilo O. De Castro, Satoshi Ohkura, Akemi Yamamoto, Yasuhiro Morita, Analyn P. Padua, Rommel C. Sulabo

The Philippine Agricultural Scientist

This experiment was conducted to determine the concentration of digestible (DE), metabolizable (ME), and net energy (NE), and to develop prediction equations for DE and ME in coconut co-products (CCP) fed to growing pigs. The CCP used were copra meal (CM), protein enriched copra meal (PECM), and white copra (WC) obtained from the different sources in the Philippines. Twenty-two growing barrows (PIC L337 × C24; initial BW 18.74 ± 0.91 kg) were individually housed in metabolism cages that allowed for a total but separated collection of feces and urine. Pigs were arranged in a replicated 11 × 3 Youden square …

Mytrials: Clinical Trial Application Concept, Katie Hollister

Graphic Communication

A clinical trial’s success is dependent upon whether a patient attends their highly-structured clinical trial visit schedule, which may include both in-person clinic visits and virtual check-in visits. MyTrials is a clinical trial journey app prototype that enables patients to schedule, reschedule, and attend join their in-person or virtual clinic visits. This product seeks to reduce the burden that scheduling places on the patient, and provides trial-provided resources and a daily and weekly timeline to help patients navigate their clinical trial journey.

Cilia Proteins Are Biomarkers Of Altered Flow In The Vasculature, Ankan Gupta, Karthikeyan Thirugnanam, Madhan Thamilarasan, Ashraf M. Mohieldin, Hadeel T. Zedan, Shubhangi Prabhudesai, Meghan R. Griffin, Andrew D. Spearman, Amy Pan, Sean P. Palecek, Huseyin C. Yalcin, Surya M. Nauli, Kevin R. Rarick, Rahima Zennadi, Ramani Ramchandran

Pharmacy Faculty Articles and Research

Cilia, microtubule-based organelles that project from the apical luminal surface of endothelial cells (ECs), are widely regarded as low-flow sensors. Previous reports suggest that upon high shear stress, cilia on the EC surface are lost, and more recent evidence suggests that deciliation—the physical removal of cilia from the cell surface—is a predominant mechanism for cilia loss in mammalian cells. Thus, we hypothesized that EC deciliation facilitated by changes in shear stress would manifest in increased abundance of cilia-related proteins in circulation. To test this hypothesis, we performed shear stress experiments that mimicked flow conditions from low to high shear stress …

A Semi-Physiological Three-Compartment Model Describes Brain Uptake Clearance And Efflux Of Sucrose And Mannitol After Iv Injection In Awake Mice, Behnam Noorani, Ekram Ahmed Chowdhury, Faleh Alqahtani, Md Sanaullah Sajib, Yeseul Ahn, Ehsan Nozohouri, Dhavalkumar Patel, Constantinos Mikelis, Reza Mehvar, Ulrich Bickel

Pharmacy Faculty Articles and Research

Purpose

To evaluate a three-compartmental semi-physiological model for analysis of uptake clearance and efflux from brain tissue of the hydrophilic markers sucrose and mannitol, compared to non-compartmental techniques presuming unidirectional uptake.

Methods

Stable isotope-labeled [¹³C]sucrose and [¹³C]mannitol (10 mg/kg each) were injected as IV bolus into the tail vein of awake young adult mice. Blood and brain samples were taken after different time intervals up to 8 h. Plasma and brain concentrations were quantified by UPLC-MS/MS. Brain uptake clearance (K_in) was analyzed using either the single-time point analysis, the multiple time point graphical method, …

Characterization Of Site-Specific Vegetation Activity In Alaskan Wet And Dry Tundra As Related To Climate And Soil State, Michael Gregory Brown, Kyle C. Mcdonald, Reiner Zimmermann, Nicholas Steiner, Stephanie Devries, Laura Bourgeau-Chavez

Michigan Tech Publications, Part 1

We present discrete (2-h resolution) multi-year (2008–2017) in situ measurements of seasonal vegetation growth and soil biophysical properties from two sites on Alaska's North Slope, USA, representing dry and wet sedge tundra. We examine measurements of vertical active soil layer temperature and soil moisture profiles (freeze/thaw status), woody shrub vegetation physiological activity, and meteorological site data to assess interrelationships within (and between) these two study sites. Vegetation phenophases (cold de-hardening start, physiological function start, stem growth start, stem growth end, physiological function end, cold hardening completion) were found to have greater interannual day of year (DOY) occurrence variability at the …

Inhibition Of Interferon-Gamma-Stimulated Melanoma Progression By Targeting Neuronal Nitric Oxide Synthase (Nnos), Shirley Tong, Maris A. Cinelli, Naglaa Salem El-Sayed, He Huang, Anika Patel, Richard B. Silverman, Sun Yang

Pharmacy Faculty Articles and Research

Interferon-gamma (IFN-γ) is shown to stimulate melanoma development and progression. However, the underlying mechanism has not been completely defined. Our study aimed to determine the role of neuronal nitric oxide synthase (nNOS)-mediated signaling in IFN-γ-stimulated melanoma progression and the anti-melanoma effects of novel nNOS inhibitors. Our study shows that IFN-γ markedly induced the expression levels of nNOS in melanoma cells associated with increased intracellular nitric oxide (NO) levels. Co-treatment with novel nNOS inhibitors effectively alleviated IFN-γ-activated STAT1/3. Further, reverse phase protein array (RPPA) analysis demonstrated that IFN-γ induced the expression of HIF1α, c-Myc, and programmed death-ligand 1 (PD-L1), in contrast …

Barriers In Surgical Research: A Perspective From The Developing World, Nadeem Ahmed Siddiqui, Muhammad Aanish Raees, Rehan Nasir Khan, Farhan Zafar

Department of Surgery Docs

Research in surgery has led to significant advances over the last century in terms of how medicine is practised in and outside the operating rooms today. Surgical research in the developed countries is responsible for most of this advancement, but it is often inapplicable in resource-limited settings in the developing world. Lower- and middle-income countries are in a unique position to take this work further, but they are limited by certain barriers. These barriers could broadly be classified under social and cultural, infrastructure, financial, ethical, and personal categories. These barriers are often not fully realised, but can potentially be addressed …

Snp-Based Breeding For Broiler Resistance To Ascites And Evaluation Of Correlated Production Traits, Katie Pepper Lee, Nicholas B. Anthony, Sara K. Orlowski, Douglas D. Rhoads

Biological Sciences Faculty Publications and Presentations

Background

The goal of this study was to evaluate marker-assisted selection (MAS) in broiler chickens using previously mapped gene regions associated with ascites syndrome incidence. The second-generation MAS products were assessed for impact on ascites phenotype and whether there were associated changes in important production traits. Previously, we used whole genome resequencing (WGR) to fine-map 28 chromosomal regions as associated with ascites phenotype in our experimental ascites broiler line (Relaxed, REL) based on a hypobaric chamber challenge. Genotypes for single nucleotide polymorphisms (SNPs) in mapped regions on chromosomes 2 and 22, were used for MAS in our REL line. After …

Cold Shock Induces A Terminal Investment Reproductive Response In C. Elegans, Leah Gulyas, Jennifer R. Powell

Biology Faculty Publications

Challenges from environmental stressors have a profound impact on many life-history traits of an organism, including reproductive strategy. Examples across multiple taxa have demonstrated that maternal reproductive investment resulting from stress can improve offspring survival; a form of matricidal provisioning when death appears imminent is known as terminal investment. Here we report a reproductive response in the nematode Caenorhabditis elegans upon exposure to acute cold shock at 2 °C, whereby vitellogenic lipid movement from the soma to the germline appears to be massively upregulated at the expense of parental survival. This response is dependent on functional TAX-2; TAX-4 cGMP-gated channels …

Self-Conscious Emotions And The Right Fronto-Temporal And Right Temporal Parietal Junction, Adriana Lavarco, Nathira Ahmad, Qiana Archer, Matthew Pardillo, Ray Nunez Castaneda, Anthony Minervini, Julian Keenan

Department of Biology Faculty Scholarship and Creative Works

For more than two decades, research focusing on both clinical and non-clinical populations has suggested a key role for specific regions in the regulation of self-conscious emotions. It is speculated that both the expression and the interpretation of self-conscious emotions are critical in humans for action planning and response, communication, learning, parenting, and most social encounters. Empathy, Guilt, Jealousy, Shame, and Pride are all categorized as self-conscious emotions, all of which are crucial components to one’s sense of self. There has been an abundance of evidence pointing to the right Fronto-Temporal involvement in the integration of cognitive processes underlying the …

Bovine Respiratory Microbiota Of Feedlot Cattle And Its Association With Disease, Jianmin Chai, Sarah F. Capik, Beth Kegley, John T. Richeson, Jeremy G. Powell, Jiangchao Zhao

Animal Science Faculty Publications and Presentations

Bovine respiratory disease (BRD), as one of the most common and costly diseases in the beef cattle industry, has significant adverse impacts on global food security and the economic stability of the industry. The bovine respiratory microbiome is strongly associated with health and disease and may provide insights for alternative therapy when treating BRD. The niche-specific microbiome communities that colonize the inter-surface of the upper and the lower respiratory tract consist of a dynamic and complex ecological system. The correlation between the disequilibrium in the respiratory ecosystem and BRD has become a hot research topic. Hence, we summarize the pathogenesis …

A Nationwide Virtual Research Education Program For Medical Students In Pakistan: Methodological Framework, Feasibility Testing, And Outcomes, Ali Aahil Noorali, Maha Inam, Hamna Shahbaz, Hareem Rauf, Faiqa Binte Aamir, Farah Khalid, Saadia Abbas, Abdullah Saeed, Muhammad Daniyal Musharraf, Asma Altaf Hussain Merchant, Babar Hasan, Muneera A. Rasheed, Fyezah Jehan, Muhammad Tariq, Adil Hussain Haider

Medical College Documents

Introduction: Equipping young medical trainees with fundamental research skills can be a promising strategy to address the need for professionals who can understand and responsibly communicate evolving scientific evidence during a pandemic. Despite an ardent interest to partake in research, most educational institutions in Pakistan and other low-middle income countries have not yet adopted a comprehensive strategy for research skills education. The authors aimed to design and assess the feasibility of implementing the first nation-wide virtual research workshop for medical students in Pakistan.
Methods: The course "Beginners Guide to Research," designed as a nation-wide virtual research workshop series, was conducted …

Comparison Of Percutaneous Vs Oral Infection Of Hamsters With The Hookworm Ancylostoma Ceylanicum: Parasite Development, Pathology And Primary Immune Response, Richard D. Bungiro, Lisa M. Harrison, Blaise Dondji, Michael Cappello

Biology Faculty Scholarship

Background

Hundreds of millions of people in poor countries continue to suffer from disease caused by bloodfeeding hookworms. While mice and rats are not reliably permissive hosts for any human hookworm species, adult Golden Syrian hamsters are fully permissive for the human and animal pathogen Ancylostoma ceylanicum. Similar to humans, hamsters may be infected with A. ceylanicum third-stage larvae orally or percutaneously. Oral infection typically leads to consistent worm yields in hamsters but may not accurately reflect the clinical and immunological manifestations of human infection resulting from skin penetration.

Methodology/Principal findings

In this study we compared host responses …

People-Powered Research And Experiential Learning: Unravelling Hidden Biodiversity, Melanie Pivarski, Matt Von Konrat, Tom Campbell, Numerous Other Co-Authors

Biology Faculty Publications

Globally, thousands of institutions house nearly three billion scientific collections offering unparallelled resources that contribute to both science and society. For herbaria alone - facilities housing dried plant collections - there are over 3,000 herbaria worldwide with an estimated 350 million specimens that have been collected over the past four centuries. Digitisation has greatly enhanced the use of herbarium data in scientific research, impacting diverse research areas, including biodiversity informatics, global climate change, analyses using next-generation sequencing technologies and many others. Despite the entrance of herbaria into a new era with enhanced scientific, educational and societal relevance, museum specimens remain …

Ephemeroptera Culturing Methods: An Analysis On Rearing And Toxcity Testing On Sensitive Early Life Stages Of Native Mayfly Taxa, Geneve Rainette Edwards

Theses, Dissertations and Capstones

Standard toxicity testing organisms are utilized for regulatory purposes and often ecological risk assessments. Ephemeroptera taxa have been reported to be more sensitive to aquatic contaminants than the standard toxicity testing organisms currently used in determining effects on aquatic ecosystems. Establishing methods for culturing native Ephemeroptera taxa will provide a more sensitive test organism to determine the toxicity of contaminants and will be more representative of the responses of native taxa. Additionally, it will provide a test organism at the most sensitive life stages. The objective of this research is to develop methods for culturing and testing of native Ephemeroptera …

Fish And Invertebrate Use Of Restored Vs. Natural Oyster Reefs In A Shallow Temperate Latitude Estuary, Jonathan H. Grabowski, Christopher J. Baillie, Adam Baukus, Rachael Carlyle, F. Joel Fodrie, Rachel K. Gittman, A. Randall Hughes, David L. Kimbro, Juhyung Lee, Hunter S. Lenihan, Sean P. Powers, Kevin Sullivan

University Faculty and Staff Publications

Coastal marine habitats continue to be degraded, thereby compelling largescale restoration in many parts of the world. Whether restored habitats function similarly to natural habitats and fully recover lost ecosystem services is unclear. In estuaries, oyster reefs have been degraded by multiple anthropogenic activities including destructive fishing practices and reduced water quality, motivating restoration to maintain oyster fisheries and other ecosystem services, often at relatively high cost. We compared fish and invertebrate communities on recently restored (0–1 year post-restoration), older restored (3–4 years post-restoration), and natural oyster reefs to determine if and when restored reefs support functionally similar faunal communities. …

Population Dynamics, Relative Abundance, And Habitat Suitability Of Adult Red Drum (Sciaenops Ocellatus) In Nearshore Waters Of The North-Central Gulf Of Mexico, Crystal Hightower, J. Marcus Drymon, Amanda E. Jefferson, Matthew B. Jargowsky, Emily A. Seubert, Simon Dedman, John Mareska, Sean P. Powers

University Faculty and Staff Publications

In the Gulf of Mexico, the red drum (Sciaenops ocellatus) is an immensely popular sportfish, yet the Gulf of Mexico stock is currently managed as data-limited in federal waters. The results of the federal stock assessment conducted in 2016 for Gulf of Mexico red drum were not recommended for providing management advice. Consequently, we sought to address data gaps highlighted in the assessment by producing up-to- date overall and sex-specific growth models, standardized indices of relative abundance, and predictions of habitat suitability and by updating estimates of natural mortality. Using a time series for the period of 2006–2018, we assigned …

Ephemeroptera Culturing Methods: Development Of Culturing And Rearing Methodology And High Sulfate Simulated Mine Effluent Toxicity Test Using Field Collected Water, Daniel Edward Brady

Theses, Dissertations and Capstones

Ephemeroptera taxa are not frequently used in toxicity testing; however, some mayfly taxa may be more sensitive to aquatic pollutants than standard test organisms used to determine anthropogenic effects on aquatic ecosystems. Additionally, some standard test organisms are not native to the Appalachian region and may not be truly reflective of the effects on native organisms. With mayflies not being the typical test organism, there is not a great deal of literature on culturing methods for this organism. For a standard acute toxicity test, there must be 80% survival within the control organisms for the test to be considered viable. …

Evaluating Relative Abundance, Fish Length, And Marine Protected Area Effectiveness For Four Key Rocky Reef Species Along The Northern Californian Coast, Leon Davis

Cal Poly Humboldt theses and projects

Marine protected areas (MPAs) were created with the purpose of helping conserve and restore diminished populations of marine organisms. Measuring the effectiveness of MPAs requires long-term monitoring, investigating the abundance and size distributions of the species that utilize the conservation areas, and comparing the results to neighboring reference sites that are not currently protected. In this study, observations from long-term MPA monitoring in northern California (2010-2019) were modeled with substrate, oceanographic, spatial, temporal, and body size variables to describe the variability in abundance and size of three fish groups: Black rockfish (Sebastes melanops), the Blue rockfish group (comprised …

Screening Ocean Samples From Georgia For The Presence Of Msx And Dermo Using Pcr And Qpcr Methods, Olivia Michelle Williams

Honors Theses and Capstones

This Senior Thesis project aimed to be able to screen samples that came in from a PhD student in Georgia for the presence of two oyster diseases, MSX and Dermo. This study was done using PCR followed by visualizing on E-gels for initial presence of disease. These were then followed by qPCR and visualization on Criterion gels. These Criterion gels were able to be analyzed in order to calculate the pg/uL of MSX and Dermo in the original ocean samples. Since the samples were sent in over the course of February-August ‘21 the presence over time was also able to …

Modernizing Fish Surveys: Evaluating How Reliable Environmental Dna Sampling Is In Monitoring Fish Populations, Alicia Cozza

EWU Masters Thesis Collection

No abstract provided.

An Assessment Of Geographic And Taxonomic Biases In Research On Climate Change-Related Range Shifts, Evan J. Parker

Library Map Prize

Geographic and taxonomic biases in ecological literature inhibit the ability of research to be generalized or translated to other systems. This is particularly true with research on climate change-related range shifts, where a diverse and representative set of observations is needed to understand the significant heterogeneity of range shifts and improve models. A literature review was conducted and biases were assessed by examining the geographic location of study areas, the taxonomic makeup of study species, and the location of study areas within species ranges. A substantial geographic skew towards terrestrial, mid-latitude North America and Europe was observed. However, taxonomic coverage …

Conditioned Territory Defense In Madagascar Hissing Cockroaches (Gromphadorhina Portentosa), Macy R. Albaitis

Honors Theses

In male blue gourami fish (Trichogaster trichopterus), signaled presentation of a male rival produces an aggressive conditioned response that results in an increased likelihood of the male winning the contest (Hollis, 1984). I replicated this classic study in a species that also shows territorial aggression: the Madagascar hissing cockroach (MHC; Gromphadorhina portentosa). During training, four adult male MHCs were designated as “CS+” animals and received a light (NS) followed by visual access to a rival male (US). Simultaneously, another group of four adult male MHCs designated as “UNC” animals received the light (NS) and visual access to a …

Increasing Detection Sensitivity For Rare And Endangered Species In Kansas Through Development Of An Aquatic Environmental Dna Sampling Protocol, Sarah Hallyburton

Master's Theses or Doctor of Nursing Practice

Anthropogenic global climate change is forcing the mass extinction of wildlife across the globe. Humans depend on freshwater ecosystems for many aspects of life including agricultural production, sanitation, and recreation. Healthy levels of biodiversity in freshwater environments ensure economically beneficial ecosystem services are maintained. Freshwater ecosystems are hotspots for biodiversity and have higher levels of imperiled organisms compared to terrestrial and marine systems. Within freshwater systems, freshwater mussels (Family: Unionidae) are the most imperiled with over 70% of North American species assigned to a conservation class as judged by state or federal management agencies. The objective of this study was …

Development Of Novel Pyridazine Derivatives And Drug Delivery Systems Against Dengue, Janae A. Culmer

College of Graduate Studies: Theses & Dissertations

The lack of approved vaccines, medications and treatment regimens has significantly contributed to the rapid spread of mosquito-borne viruses such as Dengue and Zika virus. The complex immunopathology of these viruses presents limitations for the development and implementation of a definitive, safe and effective approach to combat infections.Previous research has demonstrated that vector control strategies such as the elimination of larval habitats, larviciding with insecticides, the use of biological agents and the application of adulticides have been unsuccessful in the reduction of viral transmission leading to the need for the continued development of antivirals. This research proposes an approach for …