Research Methods in Life Sciences Commons^™

Targeting Plasma Membrane Phosphatidylserine Content To Inhibit Oncogenic Kras Function, Walaa E. Kattan

Dissertations and Theses (Open Access)

The small GTPase KRAS, which is frequently mutated in human cancers, must be localized to the plasma membrane (PM) for biological activity. We recently showed that the KRAS C-terminal membrane anchor exhibits exquisite lipid-binding specificity for select species of phosphatidylserine (PtdSer). We therefore investigated whether reducing PM PtdSer content is sufficient to abrogate KRAS oncogenesis. Oxysterol-related binding proteins ORP5 and ORP8 exchange PtdSer synthesized in the ER for phosphatidylinositol-4-phosphate (PI4P) synthesized in the PM. We show that depletion of ORP5 or ORP8 reduced PM PtdSer levels, resulting in extensive mislocalization of KRAS from the PM. Concordantly, ORP5 or ORP8 depletion …

Modulation Of Glucose Homeostasis By Nucleotide P2y2 Receptor And Biological Sex, Hailee Anne Marino

Graduate Theses/Dissertations

Recent insights into the pathological role of Nucleotide P2Y₂ receptor suggest P2Y₂R involvement in high fat diet-induced obesity and potentiates insulin resistance. However, these recent insights do not demonstrate how P2Y₂R modulates glucose homeostasis under physiological conditions. Further, it remains unknown how sex biological factors influence P2Y₂R receptor signaling in the regulation of glucose homeostasis. The research objective for the present study is to elucidate the novel roles of P2Y₂ in fasting blood glucose and glucose tolerance (basal insulin sensitivity) under resting conditions in males and females. We expected that under physiological …

Combination Of Oncolytic Adenoviruses, T-Cell Activation, And Blockade Of Ido Metabolic Circuitry For The Treatment Of Glioma, Teresa Nguyen

Dissertations and Theses (Open Access)

Glioblastoma is the most common malignant primary brain tumor in adults; the current aggressive treatment results in a 5% five-year survival rate. More effective therapies should be developed. One promising alternative is oncolytic adenovirus, Delta-24-RGD, which elicits cancer cell lysis and immunogenic cell death. In fact, Delta-24-RGD produced complete responses in 20% of recurrent glioblastoma patients through immune mechanisms that activate anti-tumor cytotoxic properties of T-cells. This cytolytic effect can further be enhanced by adding immune agonists, namely OX40L, which engages the OX40 receptor to co-stimulate activated T cells for enhanced proliferation. Hence, we produced the next generation of Delta-24-RGD, …

Developing Pandemic Comics For Youth Audiences, Judy Diamond, Amy Spiegel, Patricia Wonch Hill, Elizabeth Vanwormer, Judi M. Gaiashkibos, Bob Hall, Aaron Sutherlen, Julia Mcquillan

University of Nebraska State Museum: Staff and Affiliates, Publications

In spring 2020 our team received funding from the Rapid Response Research program of the National Science Foundation to develop comics that would help youth understand the COVID-19 pandemic. Our project built on a decade of expertise creating comics about the biology of viruses. In collaboration with virologists and artists, we developed three comic stories about COVID-19 during the pandemic and posted them on-line during the last half of 2020. The fictional narratives address fundamental issues in biology, virology, and network science, in order to help readers understand the complexities of living through a viral pandemic. The stories focus on …

Flow Cytometry For Identification Of Prdm1-Eyfp Transgenic Mice, Kayla M. Hufford, Olyvia D. Weaver, Nicole M. Young, Aspen E. King, Gregory K. Dekrey

Ursidae: The Undergraduate Research Journal at the University of Northern Colorado

The goal of this study was to determine if flow cytometry could be used to identify transgene carrier mice [B6.Cg-Tg(Prdm1-EYFP)1Mnz/J] by their expression of the enhanced yellow fluorescent protein (eYFP) in peripheral blood lymphocytes. In these mice, eYFP expression is under the control of the Prdm1 gene promoter. Littermates were identified as being either wild type or transgene carriers using a polymerase chain reaction (PCR) analysis of tail tissue. Peripheral blood leukocytes were isolated from each of the mice, and both the percent and mean fluorescence intensity (MFI) were determined for eYFP expression by lymphocytes. We found that blood lymphocytes …

Directed Genome Evolution To Identify Genes For Macrophage Survival By Staphylococcus Agnetis, Sonali Lakshika Anne Lenaduwe Lokuge

Graduate Theses and Dissertations

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a debilitating infection that negatively impacts animal welfare and costs the broiler industry billions of dollars annually. We have previously isolated Staphylococcus agnetis 908 from BCO samples obtained from broilers at the University of Arkansas research farm. This isolate can induce BCO lameness at greater than 50% in broilers exposed to the pathogen in drinking water. We found that S. agnetis 908 is capable of surviving and escaping macrophages compared to a closely related cattle isolate,1379. Through Directed Genome Evolution (DGE) we identified that this difference is at least partially associated with an alanine …

Host-Parasite Interactions Within Food Webs, Adam Zvanut Hasik

Graduate Theses and Dissertations

Parasitism is one of the most common life history strategies employed in nature, yet the effects of parasites are often thought to be minimal, and the vast majority of studies fail to consider parasites and their effects on host organisms. This is likely a problem, as the magnitude of parasite-mediated effects on their hosts can be quite large. Additionally, the effects of parasites are known to extend beyond the host to affect other species interactions. I used a series of approaches to gain a more integral understanding of host-parasite interactions by studying (1) the effects of parasites on biotic interactions …

Microfabrication And Electrochemical Characterization Of A Novel Su-8 Probe With An Array Of Individually Addressable Electrodes Suitable For Redox Cycling Experiments In Ultra-Small Volumes, Mahsa Lotfi Marchoubeh

Graduate Theses and Dissertations

Redox cycling is an electrochemical technique that utilizes closely spaced generator and collector electrodes to cycle reversible redox species between their oxidative states. With advantages in signal amplification, selectivity of species based on their electrochemical reaction mechanism, and limited or no background subtraction, this technique is well suited for selective detection of important electrochemically active molecules such as dopamine at basal or slowly changing levels.

Miniaturized medical devices have become an area of great interest for measurement of chemicals in limited volumes with low concentrations or in sensitive tissues. A probe on a polymeric SU-8 substrate with suitable dimensions and …

The Neurological Asymmetry Of Self-Face Recognition, Aleksandra Janowska, Brianna Balugas, Matthew Pardillo, Victoria Mistretta, Katherine Chavarria, Janet Brenya, Taylor Shelansky, Vanessa Martinez, Kitty Pagano, Nathira Ahmad, Samantha Zorns, Abigail Straus, Sarah Sierra, Julian Keenan

Department of Biology Faculty Scholarship and Creative Works

While the desire to uncover the neural correlates of consciousness has taken numerous directions, self-face recognition has been a constant in attempts to isolate aspects of self-awareness. The neuroimaging revolution of the 1990s brought about systematic attempts to isolate the underlying neural basis of self-face recognition. These studies, including some of the first fMRI (functional magnetic resonance imaging) examinations, revealed a right-hemisphere bias for self-face recognition in a diverse set of regions including the insula, the dorsal frontal lobe, the temporal parietal junction, and the medial temporal cortex. In this systematic review, we provide confirmation of these data (which are …

Science Forum: A Community-Led Initiative For Training In Reproducible Research, Susann Auer, Nele A. Haeltermann, Tracey L. Weissberger, Jeffrey C. Erlich, Damar Susilaradeya, Magdalena Julkowska, Małgorzata Anna Gazda, Benjamin Schwessinger, Nafisa M. Jadavji, Angela Abitua, Anzela Niraulu, Aparna Shah, April Clyburne-Sherinb, Benoit Guiquel, Bradly Alicea, Caroline Lamanna, Diep Ganguly, Eric Perkins, Helena Jambor, Ian Man Ho Li, Jennifer Tsang, Joanne Kamens, Lenny Teytelman, Mariella Paul, Michelle Cronin, Nicolas Schmelling, Peter Crisp, Rintu Kutum, Santosh Phuyal, Sarvenaz Sarabipour, Sonali Roy, Susanna M. Bachle, Tuan Tran, Tyler Ford, Vicky Steeves, Vinodh Ilangovan, Ana Baburamani

Agricultural and Environmental Sciences Faculty Research

Open and reproducible research practices increase the reusability and impact of scientific research. The reproducibility of research results is influenced by many factors, most of which can be addressed by improved education and training. Here we describe how workshops developed by the Reproducibility for Everyone (R4E) initiative can be customized to provide researchers at all career stages and across most disciplines with education and training in reproducible research practices. The R4E initiative, which is led by volunteers, has reached more than 3000 researchers worldwide to date, and all workshop materials, including accompanying resources, are available under a CC-BY 4.0 license …

A Qualitative Study On The Factors Affecting The Willingness Of 3rd Level Students To Register As Organ Donors In Ireland, Evelyn Hayes, Erika Horan, Cian Lally, Aisling Murphy

International Undergraduate Journal of Health Sciences

A wide range of factors contribute to an individual’s choice whether or not to register as an organ donor. The knowledge of the Irish population at large around the area of donation and transplantation is varied. A research survey was designed to be completed by third level students. The purpose of the research was to determine the most important factors that played a role in their decisions regarding opting-in to organ donation. A second aim of the survey was to determine the participants’ levels of knowledge and understanding on the ‘opt in’ donation system in place here in Ireland.

The …

Full Issue: The International Undergraduate Journal Of Health Sciences, Volume 1, Issue 1, June 2021, Iujhs Full Issue

International Undergraduate Journal of Health Sciences

The full June 2021 issue (Volume 1, Issue 1) of the International Undergraduate Journal of Health Sciences

Biodiversity Monitoring And Volunteer Motivations: A Case Study On The Imagined Communities Of Citizen Scientists In Meinung, Taiwan, Serena May Calcagno

Master's Projects and Capstones

The Asia Pacific’s biodiversity is under threat. One significant step that can improve conservation is gathering data on what species exist in different areas over time, which can provide insight into ecosystem health. This is especially important in biodiversity hotspots, where high levels of endemism and anthropogenic risk overlap. Though it is one of the few places in the Pacific not classified as a biodiversity hotspot, Taiwan has an unusually high saturation in terms of biodiversity data points. Investigating the motives of biodiversity monitoring volunteerism is already a topic of growing scholarly interest, but relatively few studies have focused on …

Larval Chondrocranial And Internal Oral Morphology Of The Neotropical Treefrog Boana Crepitans (Wied-Neuwied, 1824; Amphibia, Anura, Hylidae), Marianna Isabella Rosa Rodrigues De Oliveira, Luiz Norberto Weber, Johnny Sousa Ferreira, Anna Evelin Coimbra Liborio, André Masahide Guimaraes Takazone, Rafael O. De Sá

Biology Faculty Publications

We describe the internal oral morphology and chondrocranial anatomy for Boana crepitans tadpoles, and compare them with available descriptions for other species in the subfamily Cophomantinae. Among species of the Boana faber group, the chondrocranial anatomy has been reported only for one species internal oral morphology and cranial anatomy are similar to other described species of Boana and Cophomantinae. B. crepitans lacks unique features in the oral cavity and chondrocranium that would distinguish it from other congeneric species. We identify six characters from the internal oral anatomy of tadpoles unique for Cophomantinae. In addition, Boana has infralabial papillae projections, buccal …

Mapping The Little Brain At The Heart By An Interdisciplinary Systems Biology Team., Rajanikanth Vadigepalli, James S. Schwaber

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

No abstract provided.

Cnd-1/Neurod Is Required For Rme Head Neuron Cell Fate Specification In The Nematode Caenorhabditis Elegans., Genevieve Doxakis

Symposium of Student Scholars

cnd-1/NeuroD is required for RME head neuron cell fate specification in the nematode Caenorhabditis elegans.

Genevieve Doxakis (1), Illyana Weitzel (2), Amber Baldwin (1), Davron Hanley (1), and Martin L. Hudson (1)

(1) Department of Molecular and Cellular Biology, Kennesaw State University

(2) STEM Magnet Program, Wheeler High School

Transcription factors are proteins that control gene transcription. NeuroD is a deeply conserved transcription factor that regulates neuronal differentiation and pancreatic development. Mutations in this gene correlate with several neurological disorders, creating an imperative for further study. The nematode Caenorhabditis elegans has a NeuroD ortholog, cnd-1, which means we can use …

The Effects Of Mapk Signaling On The Development Of Cerebellar Granule Cells, Kerry Morgan

University Scholar Projects

The granule cells are the most abundant neuronal type in the human brain. Rapid proliferation of granule cell progenitors results in dramatic expansion and folding of the cerebellar cortex during postnatal development. Mis-regulation of this proliferation process causes medulloblastoma, the most prevalent childhood brain tumor. In the developing cerebellum, granule cells are derived from Atoh1-expressing cells, which arise from the upper rhombic lip (the interface between the roof plate and neuroepithelium). In addition to granule cells, the Atoh1 lineage also gives rise to different types of neurons including cerebellar nuclei neurons. In the current study, I have investigated the …

The Effects Of Mapk Signaling On The Development Of Cerebellar Granule Cells, Kerry Morgan

Honors Scholar Theses

The granule cells are the most abundant neuronal type in the human brain. Rapid proliferation of granule cell progenitors results in dramatic expansion and folding of the cerebellar cortex during postnatal development. Mis-regulation of this proliferation process causes medulloblastoma, the most prevalent childhood brain tumor. In the developing cerebellum, granule cells are derived from Atoh1-expressing cells, which arise from the upper rhombic lip (the interface between the roof plate and neuroepithelium). In addition to granule cells, the Atoh1 lineage also gives rise to different types of neurons including cerebellar nuclei neurons. In the current study, I have investigated the …

Biomedical Porcine Models For The Study Of Surgical Hemostasis, Hindlimb Ischemia, And Pancreatic Cancer, Shruthishree Aravind

Theses & Dissertations

Murine models have dominated the world of biomedical research and comparative medicine since their development in the early 1900s. [1] While they may be suitable models to study proteomics and genomics, they may not serve as effective translational models. [2-4] Murine models do not accurately model the pathophysiology of human disease and are limited by their size, application of medical imaging and intervention, which reduces their overall preclinical predictive value. [2-4]

Porcine models on the other hand, are slowly and steadily bridging the gap between murine models and human patients. [5] Pigs …

Safety Of Silver Oxide Coated Biomaterials In Mice, Michael Klug, Darien L. Seidman, Rahyan Mahmoud, Destiny Morot, Lei Yu, Jeffrey D Hettinger, Renee M Demarest

Rowan-Virtua Research Day

It has been demonstrated that silver oxide coatings designed by our collaborators are able to prevent E. coli and P. aeruginosa attachment to biomaterials in vivo. These findings demonstrate that such coatings show promise in preventing the development of biofilm on biodevices. However, it is unknown if the use of silver oxide in this fashion is toxic in vivo. The goal of this project was to determine whether our silver oxide coatings are safe to use in vivo. To assess the toxicity of our silver oxide formula, mice were implanted with either silver oxide coated titanium discs or uncoated titanium …

Evaluation Of A Keratin 1 Targeting Peptide-Doxorubicin Conjugate In A Mouse Model Of Triple-Negative Breast Cancer, Azam Saghaeidehkordi, Shiuan Chen, Sun Yang, Kamaljit Kaur

Pharmacy Faculty Articles and Research

Chemotherapy is the main treatment for triple-negative breast cancer (TNBC), a subtype of breast cancer that is aggressive with a poor prognosis. While chemotherapeutics are potent, these agents lack specificity and are equally toxic to cancer and nonmalignant cells and tissues. Targeted therapies for TNBC treatment could lead to more safe and efficacious drugs. We previously engineered a breast cancer cell targeting peptide 18-4 that specifically binds cell surface receptor keratin 1 (K1) on breast cancer cells. A conjugate of peptide 18-4 and doxorubicin (Dox) containing an acid-sensitive hydrazone linker showed specific toxicity toward TNBC cells. Here, we report the …

Pathogenesis Of Dry Eye In Graft Versus Host Disease (Gvhd): Role Of Ocular Mucins And Conjunctival Fibrosis, Kiumars Shamloo

Pharmaceutical Sciences (PhD) Dissertations

Allogenic hematopoietic stem cell transplantation is a procedure that offers a possible cure for hematologic cancers and other hematologic disorders. Unfortunately, despite the increasing survival rate of patients, the quality of their life is adversely affected by the allogeneic bone marrow transplantation’s major side effect i.e Graft vs. Host Disease (GVHD). GVHD is a complex, multi-organ disease resulting from an immunological attack by donor engrafted immune cells to host organs, including the eye surface. Mostly based on the time of disease onset after transplantation, GVHD is divided into the acute and chronic phase. The eyes may be involved in both …

Evaluation Of Weight Gain, Feed Intake, Feed Conversion And Oocyst Shedding Of Eimeria Maxima And Eimeria Acervulina In Broiler Chickens, Lauren Laverty

Poultry Science Undergraduate Honors Theses

The purpose of the present study was to evaluate the day and the time of sample collection of an

experimental challenge with Eimeria maxima (EM) and Eimeria acervulina (EA) in broiler chickens. One day old male Cobb-Vantress broiler chickens were randomly allocated to one of three groups with ten replicates (n=8 chickens/replicate). Chickens were placed in battery cages with a controlled age-appropriate environment: Group 1) Negative control (no challenge or treatment); 2) Challenge control (Eimeria challenge only); 3) Challenge + Salinomycin. Challenged chickens were orally gavaged with the mixed culture of EM/EA (10,000 sporulated EM containing 4% wild-type EA) …

Survey Of Ticks And Tick-Borne Pathogens Associated With Feral Swine (Sus Scrofa) In Arkansas, Haylee Campbell

Graduate Theses and Dissertations

Feral hogs (Sus scrofa L.) are an invasive species throughout the southeast United States and found in every Arkansas county. As feral hogs invade new habitat, they can disrupt ecosystems, damage agriculture systems, and bring ticks and tick-borne pathogens with them. There are no surveys of the tick species parasitizing the Arkansas feral hog populations or the pathogens they carry. This is a public health concern because feral hogs occupy often same geographical regions as humans and livestock and can harbor over 45 animal diseases and parasites. The ticks carried by feral hogs can carry tick-borne pathogens that can cause …

Particle Tracking Reveals Pelagic Red Crabs As Indicators Of Climate-Driven Range Expansion In The California Current, Elizabeth Saraf

Senior Honors Projects

Since their first recorded occurrence in 1859, Pelagic Red Crabs (PRC; Pleuroncodes planipes) have experienced increased frequency of episodic mass stranding events in the Eastern Pacific Ocean. PRC’s are transported over 1,000 km northward of their central domain from the coast of central Baja California, Mexico. They have been reported by the thousands on the beaches of central and southern California. Their presence influences the surrounding ecosystem and commercial fisheries because they are nutritional prey items and large predators change their diet to incorporate PRC’s when they are available. Seasonal coastal currents and the California Undercurrent transport warmer waters …

Role Of Reptile-Invertebrate Interactions In Enigmatic Reptile Declines, Meredith Swartwout

Graduate Theses and Dissertations

Reptile populations are declining world-wide and the mechanisms behind many of these declines remain enigmatic. Food web interactions (i.e., reduced prey availability or increased predation) have been implicated behind some reptile declines. However, relatively little is known about predation on lizard and snake eggs, despite egg survival being important for population dynamics of some species. Ants are important predators of squamate reptile eggs in tropical and temperate systems. In Costa Rica, long-term declines in terrestrial anole lizards were linked with reduced leaf litter depth, a factor that could influence egg vulnerability to ant predation. Fire ants (genus Solenopsis) are aggressive …

Climate-Driven Impacts On Himalayan Aquatic Biodiversity: A Case Study Involving Snowtrout (Cyprinidae: Schizothorax), Riri Wiyanti Retnaningtyas

Graduate Theses and Dissertations

Monitoring biodiversity, to include its relative dispersal and contraction, has become a conservation task of great importance, particularly given the catastrophic and ongoing loss of habitat due to climate change. However, the timing, direction, and magnitude of these rates vary across taxa and ecosystems. Predicting specific impacts of climate change can thus be difficult and this, in turn, hampers management action. Metrics are needed to not only quantify contemporary requirements of species, but also predict potential distributions that fluctuate in lockstep with climate.

Montane ecosystems in the Himalayas are highly impacted by climate change, yet remain largely understudied due to …

Aiming For The Sun- Averting The Fate Of Icarus: De-Risking Solar Energy Projects, Manu Srivastava

Asian Management Insights

Climate change is increasingly accepted as an existential threat to civilisation and ‘business as usual’ is considered a luxury we cannot afford any more.

Responses To Fine Particulate Matter (Pm2.5) Exposures In Two Wild-Type Zebrafish Strains, Connor Necaise

Honors Theses

Atmospheric fine particulate matter (PM_2.5) exposure poses great health risks across the globe, causing both acute and chronic illnesses in humans. Therefore, a more complete understanding of the mechanisms in which PM_2.5 induces these adverse health effects is urgently needed. Oxidative stress due to PAHs and other common components of PM_2.5 is a proposed mechanism for its adverse health effects. However, little is known about the actual mechanisms of PM_2.5 damage in humans. This study aimed to distinguish behavioral differences in two lines of zebrafish (AB & 5D) as a result of developmental exposure to …

The Effect Of Agkistrodon Contortrix And Crotalus Horridus Venom Toxicity On Strike Locations With Live Prey., Chase Giese

Honors Program: Senior Projects (Public)

This paper aims to uncover if there is a significant difference in the strike location of snake species that have different values of LD50% venom. It is thought that most snakes strike their prey in the anterior (head) area in order for their venom to work quicker in killing them. Venom toxicity is measured by its LD50% value, which is the amount of venom, in mg/kg, to kill 50% of a test population. The Copperhead has an LD50% value of 10.9 mg/kg, and the Timber Rattlesnake has an LD50% value of 1.64 mg/kg. The hypothesis was that if venom toxicity …