Research Methods in Life Sciences Commons^™

Sexual Dimorphism Of Glomerular Capillary Morphology In Rats, Zackarias Coker

Undergraduate Honors Theses

Chronic kidney disease (CKD) progresses faster in males than females; however, the underlying mechanisms remain poorly understood. Sex differences in glomerular capillary morphology has been hypothesized to contribute, in part, to the increased susceptibility to hypertension-induced renal injury and CKD progression in males, but this has not been investigated. The goal of the present study was to assess glomerular capillary morphology in male vs. female rats with intact kidneys and after uninephrectomy (UNX). We hypothesized that glomerular capillary radii (R_CAP) and length (L_CAP) would be greater in male rats.

Male (n=4) and female (n=4) with intact …

Abcds Of Triggerfish In Pemba And Misali Island: Abundance, Biodiversity, Behaviour, Cultural Significance And Distribution, Hannah Ng Shueh Yi

Independent Study Project (ISP) Collection

This study takes a socioecological approach to understanding triggerfish (Balistidae) in Pemba Island, using Misali Island reefs as a sample ecological site. Two reef sites on Misali Island and three of four districts in Pemba were observed during the study. The abundance of Balistipus undulatus at one of two study sites is close to the maximum carrying capacity of the reef. Overall observed biodiversity was low. Behavioural trends point to distinct species specific defence strategies and species aggression taking different forms. Triggerfishes cultural significance was primarily as a food fish, though it is not highly sought after. With polarised responses …

Rapid Assesment Of Aquatic Insect Diversity And Abundance Used To Evaluate The Quality Of Stream Restoration, Kaleb Stephen Kindoll

2023 Midwest Ecology & Evolution Conference

Stream and wetland restoration activities seek to improve the function of aquatic ecosystems. Measurement of the diversity and abundance of aquatic insects are commonly used to evaluate the success of restoration efforts, but these measurements are often very time consuming. In this study, we attempt to construct an efficient method that can be used to rapidly assess the diversity and abundance of aquatic insects as a measure of the quality of stream restoration. We suspended uniform sized pieces of cardboard paper coated with adhesive to trap insects in three different environments: one restored and two unrestored streams with similar characteristics. …

Understanding Invasive Predation: Detections Of Feral Cats (Felis Catus) On Kangaroo Island’S Western River Refuge, Leah Boget

Independent Study Project (ISP) Collection

Feral cats (Felis catus) are a priority-level threat for small to medium-sized native vertebrates across Australia and especially so in insular environments like that of Kangaroo Island, South Australia. The island is home to several endangered and geographically restricted species, including the Kangaroo Island Dunnart (Sminthopsis aitkeni), an endangered marsupial endemic to Kangaroo Island. When bushfires spread across the island and burned x percent of native vegetation in late 2019 to early 2020, these populations were critically impacted and post-fire feral cat predation on the island risked their extinctions, motivating the creation of a refuge with …

Impact Of Wildlife Provisioning On Species Diversity, Relative Frequency, And Richness In New South Wales, Alanah Cohen-Tigör

Independent Study Project (ISP) Collection

Wildlife provisioning and camera trapping are two recently evolving methods of conservation and wildlife management, aimed at protecting animals in the face of ever worsening climate change. Habitats are being destroyed by climate disruption, decreasing species diversity and abundance worldwide. It is imperative that methods of protecting species are developed to slow or reverse this era of extinction. By providing supplementary food and water to ecological communities, the hope is that species will have improved survivorship and reproduction, making them more resilient and resistant to population decline. Camera trapping is product of modern technology, allowing researchers to monitor species without …

A Comparative Plague Study Of Cacao Fungal Disease In Cacao Pods Within Monocultures And Indigenous Agroforests In Ecuador’S Napo Province, Seamus Mccarthy

Independent Study Project (ISP) Collection

This study analyzes the composition of three major fungal diseases in Theobroma cacao fruits compared between monocultures and chakra agroforests in the Napo province of Ecuador with the goal of noting similarities and differences in the disease composition between the two systems, as well as investigate possible variation within this poorly understood category of agroforest to better structure future studies. Cacao pods on sampled trees were counted and fungal infections identified visually and by touch. Chakra systems were selected in the communities of Cinco de Enero and Seis de Marzo to the Southwest of Tena, Ecuador. Monoculture data was collected …

Movement, Behavior, And Trophic Ecology Of A Pelagic Predator Guild In The Eastern Tropical Pacific Ocean, Ryan Keith Logan

All HCAS Student Capstones, Theses, and Dissertations

Pelagic apex predators exert strong influences on ecological communities, and often support valuable commercial or recreational fisheries worldwide. Yet, due to their rarity and pelagic lifestyle, many species, such as billfishes, have proven particularly difficult to study at resolutions necessary to define dynamics of recovery from fishery interaction, physical interaction with environmental features and prey exploitation, and competitive interactions among other billfish predators. This leads to a paucity of knowledge on billfish ecology and habitat use, and hinders management efforts. With the ever-improving and miniaturization of technology and oceanographic datasets, the ability to define and quantify these interactions of fish …

Passive Chlorination: Providing Clean Drinking Water To Rural Ecuadorian Communities, C.D. Tatko, Kyle Borror

Summer Research

In 2002, the World Health Organization (WHO) reported that nearly 1.7 million deaths worldwide were caused by unclean water. The risk of death is elevated for young children, for which the second cause of death worldwide is diarrheal disease, which is largely caused by microbes; these microbes, when unaddressed, can proliferate in drinking water. Health risks are further elevated in rural communities, which often lack access to the technology necessary to treat water for microbial contamination. This can be seen in Ecuador, in which only 52.8% of rural households have access to safe drinking water.

Orb-Web Spider Argiope (Araneidae) As Indigenous Arrow Poison Of G/Ui And G//Ana San Hunters In The Kalahari, Tharina L. Bird, Smith Moeti, Robert K. Hitchcock, Melinda C. Kelly, Lefang L. Chobolo, Nonofo Gotcha, Kgosi K. Moatlhodi, Leungo D. Mukoka, Emmanuel K. Sekopo, Caroline Simmrita Chaboo

University of Nebraska State Museum: Staff and Affiliates, Publications

Hunting has been crucial in early human evolution. Some San (Bushmen) of southern Africa still practice their indigenous hunting. The use of poisons is one remarkable aspect of their bow-and-arrow hunting but the sources, taxonomic identifications of species used, and recipes, are not well documented. This study reports on fieldwork to investigate recent indigenous hunting practices of G/ui and G//ana San communities in the Central Kalahari Game Reserve (CKGR), Botswana. Here we discuss their use of spider poison. The hunters use the contents of the opisthosoma (‘abdomen’) of a spider as sole ingredient of the arrow poison and discard the …

A Growth Model For Arctica Islandica: The Performance Of Tanaka And The Temptation Of Von Bertalanffy—Can The Two Coexist?, John M. Klinck, Eric N. Powell, Kathleen M. Hemeon, Jillian R. Sower, Daniel R. Hennen

CCPO Publications

Organisms increase in size over time (age) due to excess assimilation over metabolic (respiration) energy demands. Most organisms reach a maximum size with increasing age as gain and loss balance. The von Bertalanffy length-at-age relationship, which is commonly used in fishery assessment calculations, imposes such a maximum size. However, some fished species, such as ocean quahogs, Arctica islandica, are long lived and continue to grow at old age. The Tanaka age-at-length relationship has continued growth at old age, but is rarely used in stock assessment models. A modified form of the von Bertalanffy model is presented, which mimics the …

Modeling The Bidirectional Relationship Between Shared-Patient Physician Networks And Patient Longitudinal Treatment Patterns: Application To Physician Risky-Prescribing, Xin Ran

Dartmouth College Ph.D Dissertations

Risky-prescribing is a pressing public health concern in the United States. Opioids, benzodiazepines, and non-benzodiazepine sedative-hypnotics (sedative-hypnotics) are three commonly-prescribed but potentially risky drug groups, prescribed alone or in combination. Physician shared-patient networks provide a unique perspective in studying physician network characteristics and structures, as well as their association with the delivery of health care. Understanding how physician shared-patient networks are related to their prescribing may inform network-based interventions targeting risky-prescribing, which is yet to be fully studied.

We investigated patient receipt of risky prescriptions and physician risky-prescribing intensity through the scope of shared-patient networks. We used retrospective Medicare insurance …

Orb-Weaver Diversity And Niche Partitioning In Ecuador’S Amazonian Foothills: What Spiders Can Reveal About Tree Fall Gaps, Streams, And Cultivated Areas, Riley Endries

Independent Study Project (ISP) Collection

As abundant predators at the top of arthropod food chains, spiders are excellent bioindicators. Araneae is a megadiverse and extremely understudied order, especially in the tropics. This study aims to investigate the diversity of orb-weaver spiders across a disturbance gradient and variety of habitat types as well as their microhabitat preferences and potential niche partitioning. Spider collection was performed on spiders of the families Araneidae, Tetragnathidae, and Theridiosomatidae in Sumak Kawsay in Situ biological reserve in the lower elevation cloud forest of Ecuador’s Andean foothills. Spiders and webs were observed and analyzed from primary and secondary forest tree fall gaps, …

The Nesting Behavior Of Russet-Backed Oropendola On Sumak Allpa, Ecuador: An Analysis Of Nest Construction And Interactions With Competing Species, Claire Molina

Independent Study Project (ISP) Collection

Nests are the literal place of birth of bird species; they are incredibly important in the survival and reproduction of populations. Therefore, nesting behavior is a gateway into the lives and success of the animals. With the degradation of natural landscapes due to human activity, there is more of a need to monitor these nests to ensure healthy inhabitants. The Russet-backed Oropendola (Psarocolius angustifrons) creates unique, pendulous nests that are subject to piracy, parasitism, and predation; as well as weather phenomenon’s such as wind and rainstorms. This study describes the nest construction behavior, nest materials and nest architecture by collecting …

Hummingbird Diversity, Abundance, And Feeding Interactions Across Three Floral Communities On Mount Totumas, Chiriquí, Panamá, Zachary Ginn

Independent Study Project (ISP) Collection

(Trochilidae) are a large Neotropical bird family of nectar-feeders that have evolved as pollinators of many Neotropical plants. Interactions between hummingbirds and plants form mutualistic networks that may change in structure over environmental and anthropogenic gradients. While the unique dynamics of hummingbird diversity and floral interactions have been studied throughout the Neotropics, differing drivers between locations emphasizes the need for further local research. This deficit is especially crucial in biodiverse and understudied locations like the Western Highlands of Panamá. In this study, I investigated how hummingbird diversity, abundance, and floral interactions differed between Cloud Forest, Garden, and Oak Forest on …

Observational Study Of Woolly Monkey Behavior And Vocalizations: Behavioral Time Allotment And Vocalization Habits Of A Small Population Of Poeppig’S Woolly Monkey (Lagothrix Lagotricha Poeppigii) In The Ecuadorian Amazon, Marina Smith-Hanke

Independent Study Project (ISP) Collection

Poeppig’s woolly monkey (Lagothrix lagotricha poeppigii), a species of primate native to the Amazonian regions of western Brazil, eastern Ecuador, and northeastern Peru, is classified as endangered by the IUCN. These large, frugivorous primates are threatened by habitat loss and climate change as a result of human interference. Deforestation, hunting, petroleum mining, and illegal trafficking are all huge threats to this species. As a result of these pressures, their populations have decreased by at least 30% over the past three generations. However, as keystone seed dispersers, they are essential for Amazonian ecosystem health; without their seed dispersal services, a loss …

Online Privacy Challenges And Their Forensic Solutions, Bandr Fakiha

Journal of the Arab American University مجلة الجامعة العربية الامريكية للبحوث

In the digital age, internet users are exposed to privacy issues online. Few rarely know when someone else is eavesdropping or about to scam them. Companies, governments, and individual internet users are all vulnerable to security breaches due to the challenges of online privacy ranging from trust and hierarchical control to financial losses. As systems advance, people are optimistic that forensic science will provide long-term interventions that surpass the current solutions, including setting stronger passwords and firewall protection. The future of online privacy is changing, and more practical interventions, such as email, malware, mobile, and network forensics, must be integrated, …

Assessing The Threat Of Coral Decline On Fish Diversity In Response To Temperature, Ryland Talmadge

Independent Study Project (ISP) Collection

Misali, a PECCA-protected island off the east coast of Pemba, Tanzania, is considered to be a marine biodiversity hotspot with a protected Non-Extractive Zone (NEZ) and remaining Extractive Zone (EZ). Upon protection, the island’s fringe reefs faced severe coral bleaching during the 1998 bleaching event, estimated to kill up to 70% of corals. Since the event, several others have occurred in addition to restructuring the management plan in 2006 to expand the region to 1000km. While the damage from the most recent coral bleaching event in 2020 has yet to be surveyed, this study investigates the current reef health in …

The Relationship Between Substrate Composition, Community Structure And Feeding Preferences Of Parrot Fishes (Scaridae) In Anmardub, Guna Yala Coastal Reefs, Caitlin Amman

Independent Study Project (ISP) Collection

Coral reefs are important marine ecosystems, as they support biodiversity and generate buffer zones, yet their abundance is incredibly limited globally. One of the main threats that coral reefs face is excessive macroalgal coverage, which limits sunlight from reaching corals, and thus, limits the energy that can reach them. Parrot fish (Scaridae) are keystone herbivores, as they consume macroalgae growing on corals, which aids in reef survivability. While there is previous research on the abundances, sizes, and feeding habits of Scaridae species in the Caribbean, there is no published research on how community structure and feeding preferences of parrot fishes …

A Quantitative Study Of Orchids And Their Proximate Environments Over An Elevational Gradient On The Northern Slope Of Montagne D’Ambre, Hazel Schrader

Independent Study Project (ISP) Collection

This research investigated change in orchid populations and their proximate environments on the Northern slope of Montagne d’Ambre, a subhumid forest in Northern Madagascar. Orchid density, way of life (epiphyte or terrestrial), phenology, and associations were recorded over 100m elevation gradients and between forest types (primary, secondary, dry, and humid forests at the same altitude). Additionally, orchid preferences for certain heights in the forest and DBH of host trees were analyzed. The establishment of 50X20m plots in 100m elevation gradients combined with ground survey yielded significant results demonstrating change along with elevation as well as forest types. Orchid density increased …

Fish And Invertebrate Use Of Restored Vs. Natural Oyster Reefs In A Shallow Temperate Latitude Estuary, Jonathan H. Grabowski, Christopher J. Baillie, Adam Baukus, Rachael Carlyle, F. Joel Fodrie, Rachel K. Gittman, A. Randall Hughes, David L. Kimbro, Juhyung Lee, Hunter S. Lenihan, Sean P. Powers, Kevin Sullivan

University Faculty and Staff Publications

Coastal marine habitats continue to be degraded, thereby compelling largescale restoration in many parts of the world. Whether restored habitats function similarly to natural habitats and fully recover lost ecosystem services is unclear. In estuaries, oyster reefs have been degraded by multiple anthropogenic activities including destructive fishing practices and reduced water quality, motivating restoration to maintain oyster fisheries and other ecosystem services, often at relatively high cost. We compared fish and invertebrate communities on recently restored (0–1 year post-restoration), older restored (3–4 years post-restoration), and natural oyster reefs to determine if and when restored reefs support functionally similar faunal communities. …

An Assessment Of Geographic And Taxonomic Biases In Research On Climate Change-Related Range Shifts, Evan J. Parker

Library Map Prize

Geographic and taxonomic biases in ecological literature inhibit the ability of research to be generalized or translated to other systems. This is particularly true with research on climate change-related range shifts, where a diverse and representative set of observations is needed to understand the significant heterogeneity of range shifts and improve models. A literature review was conducted and biases were assessed by examining the geographic location of study areas, the taxonomic makeup of study species, and the location of study areas within species ranges. A substantial geographic skew towards terrestrial, mid-latitude North America and Europe was observed. However, taxonomic coverage …

Review Of The Effects Of Asphalt Fume Emission (Vocs And Pahs) In Vitro, In Vivo And Human Studies, Omran Taqi, Nastassja Lewinski, Elham Fini, Eran Rozewski, Judith Klein-Seetharaman

Undergraduate Research Posters

Review of the effects of Asphalt fume emission (VOCs and PAHs) in vitro, in vivo and human studies

Omran Taqi, Nastassja Lewinski¹

1) Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, VA

Background

Asphalt is abundantly available around human operations including road paving and roofing sites. However, their emissions and fumes (specifically volatile organic compounds (VOCs) and polycyclic aromatic hydrocarbons (PAHs)) have been found to have adverse effects on health. Asphalt fumes are a class 2A carcinogen and exposure can increase the risk of lung cancer, asthma, headache, fatigue, and other cardiovascular diseases.

Methods

We used …

Systematic Review Of Polyherbal Combinations Used In Metabolic Syndrome, Amber Hanif Palla, Faridah Amin, Bilqees Fatima, Arooj Shafiq, Najeeb Ur Rehman, Ikram Ul Haq, Anwar-Ul-Hassan Gilani

Department of Biological & Biomedical Sciences

Background: Metabolic syndrome (MetS) is a multifactorial disease, whose main stay of prevention and management is life-style modification which is difficult to attain. Combination of herbs have proven more efficacious in multi-targeted diseases, as compared to individual herbs owing to the "effect enhancing and side-effect neutralizing" properties of herbs, which forms the basis of polyherbal therapies This led us to review literature on the efficacy of herbal combinations in MetS.
Methods: Electronic search of literature was conducted by using Cinnahl, Pubmed central, Cochrane and Web of Science, whereas, Google scholar was used as secondary search tool. The key words used …

A Phenological Analysis Of Páramo Vegetation Along An Altitudinal Gradient: The Influence Of Camelids On Andean Flora In The Páramo Of Chimborazo, Ecuador, Tessa Seifried

Independent Study Project (ISP) Collection

This study hopes to reveal the mechanisms behind camelid-plant interactions in the Chimborazo province of Ecuador, which is known for having a large population of domesticated alpacas (Vicugna pacos), along with wild vicuñas (Vicugna vicugna) that were reintroduced into the area 30 years ago. These camelid species, especially vicuñas, are understudied within Ecuador in regards to their effects on plant composition, diversity, and spatial phenology. To gain a better insight into this topic, three sites were chosen: a site inhabited by only vicuña, a site inhabited by only alpaca, and a site inhabited by both species. The vicuña site sat …

A Ladder Of Endemicity: A Pioneering Study Of Anuran Communities Along An Elevational Gradient In The Eastern Andean Cloud Forests, Ecuador, Ella Halbert

Independent Study Project (ISP) Collection

Per unit of area, Ecuador is home to the most herpetofauna of any region on Earth, yet many ecosystems are under researched. Positioned between the Eastern Andes and the Amazon rainforest, the Río Pastaza Basin of Ecuador has been the recent focus of anuran biodiversity surveys and is home to many endemic species. This study aimed to determine how the anuran community composition of a vertical transect in the Llanganates National Park varied between elevations. A 7-day expedition was conducted along a new trail up Abitagua Range spanning 1,050 m in elevation. Each night, visual encounter surveys were employed to …

A Biological Assessment Of Water Quality In El Placer, Ecuador: The Effect Of Agriculture On Stream Health And The Quality Of Historical Versus Current Drinking Water Sources, Danielle Kleinberg

Independent Study Project (ISP) Collection

Although fresh water is one of Ecuador’s most abundant resources, high quality drinking water for its inhabitants is scarce (Wingfield et al., 2021). The most prevalent sources of water pollution in Ecuador are domestic waste, silver and gold mining, oil production, and agricultural chemicals (Buckalew et al., 1997). El Placer, a village located in Tungurahua, Ecuador, is highly dependent on agriculture as a source of income. The first objective of this study was to determine the effect of agriculture on the El Placer’s Tía Anita Stream through comparing the water quality at three sites with varying agricultural influence. The second …

Beach Suitability For Nesting Olive Ridley Sea Turtles (Lepidochelys Olivacea), Playa Malena, Azuero Peninsula, Panama, Soleil Foy

Independent Study Project (ISP) Collection

Sea turtle species all over the world are facing continuous declines in their populations partly due to diminished viable nesting habitat. Understanding sea turtle nesting preferences and the suitability of beaches for reproductive success is necessary in order to sustain their populations and ensure their conservation. Olive ridley sea turtles (Lepidochelys olivacea) are one of the species experiencing this trend, especially in the Eastern Pacific. This study examined the beach suitability of a site known as Playa Malena in the Azuero Peninsula in Panama for Olive ridley nesting. This site was compared to the suitability of 7 other …

Abundance, Foraging Levels, And Dietary Preferences Of Chaetodon Capistratus On Reefs Surrounding Porvenir Island In The Guna Yala Comarca Of Panamá, Eleanor Ann Casement

Independent Study Project (ISP) Collection

Reef fish are a physically and functionally diverse group of organisms that live in close association with coral reef habitats. Chaetodontidae represents the most species rich family of corallivorous fish, and their reliance on corals as food resources has led to their designation as indicator species of coral cover and health. However, the majority of research on the foraging ecology of Caribbean Chaetodontids dates back several decades, and therefore does not account for recent changes in coral community composition as the result of disease, climate change, and other stressors. As the novel and deadly stony coral tissue loss disease (SCTLD) …

An Eventful Past Predicting A Bizarre Future: Assessing The Influence Of Ocean Circulation On Key Fish Species In Icelandic Waters. An Analysis Of Past Circulation Records In Order To Predict The State Of Demersal Fish Stocks In Future Climate Scenarios, Matthew Engfer

Independent Study Project (ISP) Collection

Feedback-driven changes in North Atlantic Ocean circulation are affecting marine ecosystems off the coast of Iceland and are predicted to have differing outcomes by 2100. In this study, data reflecting Icelandic fish stock distributions was compiled on a map with oceanographic temperature and depth in order to observe patterns of population changes with changes in ocean currents. After evaluating past temperature effects on demersal fish stocks, future habitat predictions were estimated in weaker ocean circulation scenarios. A number of assumptions were made in order to pinpoint a correlation between ocean temperature and fish habitat. Iceland’s unique location and climate offers …

Eradicating Zebra Mussels: What Works?, Elijah Davies

Symposium of Student Scholars

The invasion of U.S lakes and rivers by the invasive species of zebra mussels called Dreissena polymorpha has caused catastrophic harm to the local ecosystem by reproducing and outcompeting native mussel species as well as harm to pipes leading into water sources by binding to surfaces and reproducing to the point that the mussels clog pipes. In addition, recreation areas must be closed due to the sharp shells making areas unusable. In the past, research has focused on individual molluscicides and their eradication of zebra mussels, as well as their effect on native flora and fauna. My research will contrast …