Research Methods in Life Sciences Commons^™

Oral Bandage Tissue Reaction In Dogs, John Rozema

Loma Linda University Electronic Theses, Dissertations & Projects

This experiment was designed to evaluate an oral surgical dressing which may be used as an aid to healing tissue. The parameters of evaluation were directed toward wound healing enhancement, duration, and stability of the bandage. A histological evaluation of the surgical wounds protected with the bandage as compared to the control wounds was made.

The surgeries were performed on eight dogs in a double controlled experiment and were in the form of bilateral maxillary gingivectomies and mandibular molar tooth extractions. The surgery sites on the left were covered with the Orahesive Bandage while the right side remained exposed to …

Effects Of Poor Quality Diets With Various Dietary Factors Added And Exposure To Cigarette Smoke On The Voluntary Alcohol Intakes Of Albino Rats, Bonnie Emmerson

Loma Linda University Electronic Theses, Dissertations & Projects

Studies were conducted utilizing albino rats to determine the relationship of voluntary alcohol consumption with various dietary additives and exposure to cigarette smoke in combination with poor quality diets. Male, Sprague-Dawley rats, from weanling age to 100 grams, were given the various experimental diets and a free fluid choice of ten per cent ethanol or tap water. Fluid intakes were recorded and analyzed statistically. Individual variation was common with a generally low level of alcohol consumption when rats were fed a poor quality diet of common United States foods. The addition of caffeinated beverages and/or spices and relish caused increases …

Carbon Dioxide Fixation In Schistosoma Mansoni, Clint Earl Carter

Loma Linda University Electronic Theses, Dissertations & Projects

Adults of the blood fluke Schistosoma mansoni were perfused from the hepatic portal system of CF1 female mice sixty to seventy days post infection. The worms were then incubated in Eagle’s minimal essential medium with glutamate and 0.03 mg/100 ml of nonlabelled aspartic acid. To this medium was added 10 μc of either NaH¹⁴0₃, glucose-UC¹⁴ or aspartate-4-C¹⁴. The worms were found to fix carbon from the NaH¹⁴0₃ and to incorporate the glucose and aspartate. The amount of activity recovered in five different chemical fractions after two hours incubation was determined …

Mandibular And Alveolar Growth In Dog Following Infusion Of 5-Fluorouricil, Richard D. Pfeiffer

Loma Linda University Electronic Theses, Dissertations & Projects

The action of a chemotherapeutic agent as a tool in studying its effect upon a primary growth center, the mandibular condyle, was investigated. So far as can be ascertained, no studies have been reported where these growth sites have been eliminated or modified by the use of chemotherapeutic growth inhibitors.

Observation of mandibular and alveolar growth following the infusion into the left external carotid artery of a cancer chemotherapeutic agent, 5-Fluorouricil (Roach-Hoffman), was noted. Comparisons were made between the infused and the uninfused sides of the mandible observing gross growth changes.

Four dogs, aged 75 days, were infused with varying …

The Effect Of Schistosoma Mansoni On Free Amino Acid Levels In Mice, Richard D. Tkachuck

Loma Linda University Electronic Theses, Dissertations & Projects

Free amino acid levels in the blood plasma of mice infected with Schistosoma mansoni Sambon, 1907, were measured at 3, 6, and 9 weeks post-infection with an automatic amino acid analyzer. Significant differences between experimental and control animals occurred at all three time periods. At 3 weeks, asparagine, alanine, citrulline, proline, methionine, and threonine levels all were lower in the infected animals. At 6 weeks, four differences were noted. Alpha-amino-n-butyric acid, ornithine, lysine, and histidine all had lower values in infected animals. At 9 weeks there were 12 differences. Tyrosine, phenylalanine, threonine, serine, proline, citrulline, glycine, alanine, valine, isoleucine, and …

The Fecal Shedding Of Listeria Monocytogenes By Conventional And "Germ Free" Mice, Margaret Joyce Ramsay

Loma Linda University Electronic Theses, Dissertations & Projects

A series of experiments involving Listeria monocytogenes was undertaken to study first the fecal shedding of Listeria by conventional mice challenged intraperitoneally with Listeria and second the fecal shedding and ability to survive of germ free mice with similar challenge. In addition, mites infesting Listeria infected mice were cultured for their possible contamination by Listeria.

Conventional mice were challenged intraperitoneally with a single near LD₅₀ dose of Listeria. Fecal Listeria were detected by collecting specimens from each mouse daily for the first 4 days and every second day thereafter. The feces was held at 7°C for 14 …

How Can We Best Help Laboratory Animals Now?, Oliver Evans

Laws and Legislation Collection

No abstract provided.

Ixodid Ticks: Possible Vectors Of Tuberculosis, Ya. A. Blagodarnyy, I. N. Blekhman, M. P. Yukunin

United States Navy: Publications

"From these tests, we established that artificially and naturally fed ticks are susceptible to the infective source and preserve tuberculosis mycobacteria in the body for a long period."

Antibiotics And The Wasting Disease In Neonatally Thymectomized Rats, Douglas M. Grignon

Loma Linda University Electronic Theses, Dissertations & Projects

The administration of the antibiotic, Chloramphenicol, to pregnant rats just prior to, and for four days after delivery, diminishes the incidence of the so-called "Wasting Disease" in neonatally thymectomized rats.

A total of 50 rats were divided into the following groups:

1. Thymectomy only---nonmedicated 17 rats
2. Thymectomy only---medicated 12 rats
3. Thymectomy-adrenalectomy---nonmedicated 5 rats
4. Thymectomy-adrenalectomy---medicated 8 rats
5. Controls---nonmedicated 4 rats
6. Controls---medicated 4 rats

Chloramphenicol was administered in the drinking water to the mothers of groups 2, 4 and 6 for about 2 days prior to delivery and 4 days after. On the day of birth or within three days after birth, the …

Sheep Breeding Problems In W.A. : The Industry's View, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

IN 1963 a short questionnaire was distributed to farmers and pastoralists seeking information about sheep breeding problems and lamb losses.

This was a fore-runner to the Department of Agriculture survey into the causes of abortion and lamb mortality which is reported elsewhere in this issue.

Dead Lambs : A Dead Loss, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

During the past year the Animal Health Laboratory has continued its survey into the causes of abortion in sheep and perinatal lamb losses.

This article records the results of this work.

LAMBS and specimens from lambs were submitted to the Animal Health Laboratory from 229 properties situated in 121 areas in this survey during 1964.

Post-mortem examination was carried out on 1,422 lambs and specimens from 61 lambs were also examined.

The Effect Of Varying Protein Intake On Trichinella Spiralis Infection In Mice, Clare K. Kwan

Loma Linda University Electronic Theses, Dissertations & Projects

Three hundred Swiss Webster albino mice three weeks of age were placed on low (8%), medium (24%), high (48%) protein levels and a commercial laboratory animal feed, Purina Chow for various lengths of time: (1) simultaneously with infection, and four weeks thereafter, (2) three weeks before infection and throughout the four-week infection period, and (3) six weeks prior to infection and during the infection period. The protein source was casein. Sixty of the 300 mice used served as noninfected controls, and the other 240 were each infected with 400 washed, suspended Trichinella spiralis larvae obtained from a rat by the …

The Effect Of Mulesing And Tailstripping At Lamb Marking On Subsequent Lamb Growth And Incidence Of Fly Strike, R J. Lightfoot, W. L. Mcgarry

Journal of the Department of Agriculture, Western Australia, Series 4

THE modified mules and tailstrip operation has been shown to be highly effective in reducing the incidence of crutch strike in sheep.

In Western Australia the operation is usually performed on ewe weaners or hoggets immediately after either crutching or shearing.

A Study Of Regeneration Of Peripheral Nerve In The Rat, James C. Jay

Loma Linda University Electronic Theses, Dissertations & Projects

Due to improvements in fixation and embedding techniques a restudy of regenerating peripheral nerve was thought advisable in order to better understand and describe the processes which take place.

Femoral nerves of white Wistar rats were used. The lesions were made by either crushing or severing the nerve. After a period of regeneration, varying from 12 hours to six days, the wound was reopened and the injured portion fixed in situ with 1% osmium tetroxide. The tissues were then removed, dehydrated, embedded and sectioned. The sections were stained and studied with a modified RCA EMU2A electron microscope.

Observations revealed that …

Free Feeding For The Control Of Rabbits, C Marshall

Journal of the Department of Agriculture, Western Australia, Series 4

IN common with most other farm work, the control of rabbits calls for a high degree of skill, patience and perseverance.

There is no short cut to success, and if results are required we must be prepared to give of our best.

Failures of control works are experienced at various times, and the reasons can generally be traced back to something we have failed to do or some short cut we have tried to take.

However, we should not lose heart from one failure. During normal farming procedure, if a farmer's crop fails he does not give up planting in …

Pharmacological Studies Of Antidiabetic Chinese Herbs, Mun Fa Lee

Loma Linda University Electronic Theses, Dissertations & Projects

Rehmannia glutinosa, Atractylus ovata, and Polygonatum chinense have all been employed by the Chinese as oral antidiabetic agents and reported by Min (1928), Kin (1931), and Li et al. (1936) to possess hypoglycemic activity. This prompted the pharmacological studies of these herbs.

Materials and methods. Their pharmacognosy was condensed from all available Chinese and scientific literature. Preliminary chemical and physical analyses included determinations for moisture, ash, crude fat, carbohydrates, protein, phosphorus, pH, alkaloids, melting point, and spectroscopy. Employing the Somogyi-Nelson method, their glycemic activity was studied in rats, rabbits, and dogs. Stress reactions were minimized. The effect …

The Effect Of Preweanling Administration Of Carbohydrates Used In Infant Formulas On Subsequent Dental Caries In White Rats, Erlinda Amor Romulo

Loma Linda University Electronic Theses, Dissertations & Projects

The high incidence of tooth decay, one of the most common diseases found in all age groups, gives a reason for the study of methods to prevent dental caries. Certain dietary factors, specifically carbohydrates, have been implicated in the decay process. Since diet influences both the oral and systemic environments in which the teeth develop, mature, age, and decay (1), many studies have been conducted to determine the effects of oral administration of different carbohydrates on dental caries. The extrinsic effects of diet on the mature erupted tooth have, however, been the major concern of the research (2).

The first …

The Effect Of Adrenal Steroid Hormones On The Protein Content Of Tumorous Livers, Duncan W. Martin

Biology ETDs

Protein is basic to the structure and function of protoplasm. Enzyme systems which regulate the rate of cellular reactions are primarily composed of protein and many of these enzymatic systems influence the metabolism of less specialized proteins that form the major structural material of protoplasm. It is believed that experimental procedures which modify protein composition of cells may also influence the activity of intra-cellular enzymes. In experimental live cancer, it has been shown that the activities of a number of oxidative enzymes are modified as the carcinogenic process proceeds (Richmond, 1955, p.38). Furthermore, it has been demonstrated that endocrine function …

A Study Of The Renal Vascular Supply In The Domestic Cat, Elizabeth Rosen

Bachelors’ Theses

Studies on the normal and abnormal vascularization of the renal vessels in the domestic cat (felis domestica) indicate the frequency of anomalies that occur, but in most of the studies it is the inferior vena cava that is most often recorded. Among the occasional references to several renal veins or arteries we have Huntington and McClure, Elmer Butler, Gaylord Bates, William Darrach, and C.H. Fortune. Yet no study seems to have been made of individuals of any particular class. A superficial observation seems to indicate a large number of cats used in the laboratory had multiple renal veins. Hence a …

The Effect Of Adrenaline Injection On Glycogenolysis And Glycogenesis In Rat Livers, Virginia Mary Zingsheim

Bachelors’ Theses

Present day knowledge concerning the liver is derived from many sources and the accumulation of many methods of investigation. Sugar, which is needed by the body for energy, is stored in the liver as glycogen. Claude Bernard (26), an eminent physiologist, was the first to suspect that carbohydrates were stored in the liver. Faulty glycogen synthesis and hydrolysis results in one of our most widespread diseases, diabetes. Thus, many biochemists and physiologists have tried to find the mechanism of the process, and the primary and secondary facts which affect glycogenesis and glycogenolysis. Although the chemical secretions and hormones of such …

The Spinal Nerves And Spinal Cord Of The Frog, Laverne A. Penn

Bachelors’ Theses

The common grass frog, Rana pipiens as wall as the bull frog, Rana catesbiana have served during many decades as material from which both the student of zoology and the investigator in biological problems have gathered valuable information about the anatomy and physiology of the common systems. Among these systems the central, peripheral, and sympathetic nervous system plays a coordinating role, because of the ramifying nature of its organization. It is the purpose of this thesis to investigate the gross and histological structure of these branches known as spinal nerves. When one inspects the literature on the nervous system of …

The Lungs And Respiration In The Frog, Peter P. Strassburg

Bachelors’ Theses

In a wide sense, respiration is sometimes defined as the gaseous exchange constantly taking place between an organism and its environment. Essentially it is concerned with the intake of oxygen and the output of carbon dioxide but it also includes any other gas exchange that might occur. Small organisms take oxygen directly from the air or from the watery medium surrounding them into all parts of their structures. They also give off carbon dioxide from all their parts directly into the surrounding medium. They can do this successfully because they are small enough to have every cell in direct or …

Kidney Of The Frog, Felix Taubman

Bachelors’ Theses

The frog has been used exclusively in both high school and college as the main source of experimental biological and zoological studies. Because it is so easily obtained and so finely adapted to our purposes, we chose it as the specimen upon which to do our thesis work. Much has been done experimentally on the kidneys with the frog. The purpose of this thesis is to present a general view of the external and microscopic anatomy of the frog kidney and with this as a groundwork to present the problems of secretion; whether secretion is carried on by the renal …

Chelonian Brain-Membranes, Brain-Bladder, Metapore And Metaplexus, John P. Munson

Biology Faculty Scholarship

The chelonian brain is not too small to be studied macroscopically. It is easily removed from the skull. Minute surface details and the cell structure can be easily observed.