Research Methods in Life Sciences Commons^™

Ethical Aspects Of Animal Experimentation, Wolfgang Scharmann

Experimentation Collection

If inquiries are made of people regarding their attitudes towards animal experimentation, there will no doubt be various answers corresponding to the different ethical attitudes today. Three principle points of view are imaginable. The two extremes are: an unrestricted support of all animal experiments; and a radical rejection of any such experiment. These two positions, in all likelihood, are taken by only a minority of the population. The majority will approve of animal experimentation in principle, however, only insofar as it is really necessary to preserve human life. So, the point of controversy arises from the question: When is an …

Three Blind Mice, See How They Run: A Critique Of Behavioral Research With Animals, Michael A. Giannelli

Experimentation Collection

Animal research has been a traditionally accepted and respected part of modern psychology from its earliest days. The prevalent view of animals in contemporary psychology has origins far more basic than the scientific method. Its roots are deeply imbedded in Judaeo-Christian culture, a tradition which postulates a wide gulf between humankind and the animal world. The Darwinian revolution and the ethological outlook it fostered, while of immense biological significance, has for the most part been neglected by modern American comparative psychologists in favor of a positivistic-behaviorist orientation with a heavy reliance upon laboratory experimentation.

In recent years, opposition to animal …

Anthropomorphism Is Not A Four-Letter Word, Randall Lockwood

Experimentation Collection

Most animal scientists are directly or indirectly instructed to avoid any hint of anthropomorphism, yet it is an approach that is invariably applied by scientists and lay people alike. Science may have gained some objectivity in discarding this common view of animals, but it has gone overboard in its rejection of the concept of the continuity of human and animal experiences. I hope to outline how an anthropomorphic perspective can be applied in ways that are a service to science and the animals themselves.

The Imbalance Between Experiment And Theory In Biology: The Need For Theory-Directed Modeling, M. L. Fidelman, D. C. Mikulecky

Experimentation Collection

In biological and biomedical research, the vast majority of resources are focused on conducting experiments. Most of these experiments utilize animals. Only a tiny amount of resources is spent on theory and modeling. It is our contention and the basic theme of this paper that the imbalance between theory and experiment in biology produces very poor science. The implications of which are that many of the experiments conducted have little real scientific meaning or value and, therefore, go hand-in-hand with unnecessary animal use and suffering. Given the finite resources available for research, the redirection of significant resources from an almost …

Animals In Testing: How The Cpi Is Handling A Hot Issue, Anonymous

Popular Press Items

No abstract provided.

The Role Of The Salivary Glands In Uptake Of Fluoride In Teeth And Bone, Robin E. Vance

Loma Linda University Electronic Theses, Dissertations & Projects

The cariostatic capability of fluoride is generally recognized, in addition, it may also have a beneficial effect on bone. Fluoride is thought to exercise its cariostasis through a local effect on teeth, however, the possibility exists that fluoride exerts its action through a systemic mechanism. Fluoride uptake by rat teeth and its effectiveness as a cariostatic substance was studied in parotidectomized, submaxillariectomized, and totally sialoadenectomized animals (both parotid and submaxillary glands removed). The uptake of fluoride in bone was also quantitated for comparison purposes.

Rats were injected daily with 15 micromoles of fluoride dissolved in 0.1 ml saline. The rats …

Age-Related Changes Of Neurites In Meissner Corpuscles Of Diabetic, Vicky R. Ras

Loma Linda University Electronic Theses, Dissertations & Projects

This thesis is based on a qualitative and quantitative study of the neural component of Meissner corpuscles of diabetic and non-diabetic mice. Age-related changes were also documented in these mechanoreceptors. Corpuscles from forty-six diabetic mice and forty-six non-diabetic mice ranging from 2.5 to 17 months old were studied. Forepaw index digital pads were formalin-fixed and frozen-sectioned parallel to each digit and perpendicular to the skin. Serial sections were then silver-impregnated to allow light microscopic examination of the neurites in the corpuscles.

Neurites became more coarse, tortuous, branched, varicose, and thus more complex until middle age. Corpuscle size appeared greatest at …

The Hsus Condemns Psychological Experimentation On Animals

Close Up Reports

For almost a century, millions of cats, dogs, monkeys, and other laboratory animals have fallen victim to the misguided notion that by torturing animals we may someday find the golden key that unlocks the dark corners and passageways of human psychology. Heedless of any relevance the experiments may have to the human condition or of the differences between humans and other animals, experimental psychologists are exercising unbridled on animals the whole range of suffering, from emotional trauma, like that experienced by the doomed infant monkey, to outright physical torture. Animals have been blinded and returned to the wild to test …

The Effects Of Maternal Administration Of Methadone On Beta-Endorphin Content In The Brain And Pituitary Of The Rat Progeny, Randall L. Braun

Loma Linda University Electronic Theses, Dissertations & Projects

Methadone is often used in the maintenance and detoxification of opioid-dependent pregnant women. This use of methadone during pregnancy is known to be associated with teratogenic effects observed in the progeny. The present study is an attempt to gain insight into possible pathophysiologic mechanism(s) responsible for these teratogenic effects: specifically, a relationship between maternal methadone administration and the content of the endogenous opioid peptide, beta-endorphin, in the brain and pituitary of the rat progeny.

In brief, nulliparous mature female Sprague-Dawley rats received daily injections (5 mg./kg.) of methadone hydrochloride (methadone-treated) or a similar volume of physiological saline (controls), treatment started …

Distribution And Proportion Of Immunoreactive Neuron Specific Enoclase Containing Cells In The Rat Pineal Gland, Alfonso Miranda C.

Loma Linda University Electronic Theses, Dissertations & Projects

The distribution and proportion of cytoplasm containing immunoreactive neuron specific enolase (NSE) in the rat pineal have been investigated. Step serial sections of the pineal glands of 7 male Sprague-Dawley rats were stained for NSE using an indirect immunoperoxidase method. The volumes of the pineals and of the regions containing reactive cells were derived from the distance between sections and the sum of the corresponding sectional areas of camera lucida drawings. The proportion of the NSE areas actually consisting of NSE reactive cytoplasm was measured using an image array processor.

Cytoplasm reactive for NSE was consistently localized to the posterior …

Age-Changes Of The Neuronal Component Of Meissner Corpuscles In The Mouse Digital Pad, Roger C. Mathewson

Loma Linda University Electronic Theses, Dissertations & Projects

Silver impregnated sections of mouse digital pads were studied using light microscopy to detect age-related changes of the neuronal component of Meissner corpuscles. Direct microscopic observation, photomicrographs and camera lucida tracing were utilized. From qualitative observation, the corpuscular neurites were found to undergo morphological age-related changes of diameter, tortuosity, varicosity, branching and terminal expansion size. Quantitative examination was made of the number of corpuscles, corpuscular neurites, branching neurites, cross-innervations, terminal neurite expansions, neurite intraepidermal continuations and terminal axonal processes. The number of corpuscles and neurite intraepidermal continuations decreased with age while having significant linear correlation; whereas, branching increased with age …

Vivisection And Misanthropy, George P. Cave

Experimentation Collection

Goodman recognizes that it is reasonable to question whether all experiments performed on animals really contribute to human welfare, and he even concedes that the use of animals in laboratories "could no doubt be reduced further without harm to humankind." On the other hand, it is quite clear that he is completely unaware of the sheer quantity of absolutely worthless experiments currently being conducted, ,and that he subscribes to the popular misconception, deliberately perpetrated by the research establishment, that animal experimentation is coextensive with biomedical research, thereby contributing directly to human welfare through the conquering of disease. Furthermore, Goodman seems …

Thoughtful Use Of Animals, Hyram Kitchen

Experimentation Collection

As part of a symposium held in Cincinnati entitled, "Ethical Issues Related to the Use of Research Animals," I was asked by the program director to consider whether further legislation regarding the use of animals might be necessary to ensure more thoughtful use of animals at universities. The following is my response.

A Survey Of Animal Behavior-Related Research And Teaching Activities In North American Agricultural And Veterinary Medical Colleges, W. R. Stricklin

Ethology Collection

A letter questionnaire was used to survey animal behavior-related research and teaching efforts in U.S.A. and Canadian university animal sciences departments (agriculture) and veterinary medical colleges. The objectives of the eleven questions of the survey were to identify behavior workers and to determine the current and planned levels of emphasis on research and teaching activities in domestic animal behavior. During 1981, questionnaires were mailed to 162 deans and chairmen, and 102 were answered and returned. Twenty-three persons were identified as having appointments specifically or primarily in animal behavior, and 99 persons were identified as having appointments that had some responsibilities …

Psychology And Its Animal Subjects, Kenneth J. Shapiro

Experimentation Collection

By way of introducing Psychologists for the Ethical Treatment of Animals (PsyETA) to readers of the journal, I have been asked to make some comments about the organization and, from a personal point of view, to suggest some of my own positions and views.

Observation Of Bone Formation In The Mandibular Symphysis Following Surgical Separation And Slow Mechanical Expansion In A Baboon, Robert J. Homer

Loma Linda University Electronic Theses, Dissertations & Projects

Mechanical expansion of the maxilla, at the midline suture, is a widely accepted orthodontic procedure used to gain arch width in certain types of maxillary deficient arches.

In animal research on the mandible, techniques using surgical separation at the symphysis with immediate expansion up to 12 mm, fixation with a metal splint, and autogenous bone transplanted into the expanded area, have been reported to be successful.

To date, no experiment utilizing slow mechanical expansion of the mandible at the symphysis has been reported.

In this pilot study, the mandible of a female baboon was surgically separated A-P at the symphysis. …

A Message From Pano, William G. Conway

Zoos and Aquariums Collection

The memo attached was found in an unstamped envelope with no return address on the grounds of the Bronx Zoo. It appears to have been written by a chimpanzee on assignment from a clandestine organization in Africa.

Effects Of Psycho-Physiological Stress On Captive Dolphins, Nick Carter

Conservation Collection

Morgane (1978) has stated that:

Man sees all other creatures through the narrow focus of his own knowledge and sees the whole image in distortion. We patronize animals for their incompleteness and dependence and for their fate in having taken form so far below ourselves ... a great mistake, for animals should not and cannot, be measured by man. Many are gifted with many extensions of senses we have lost or never attained .... They live by voices we may never hear. Some may not be our accepted brethren, but also they are not our underlings.

If this "narrow focus …

Reporting Requirements Under The Animal Welfare Act: Their Inadequacies And The Public's Right To Know, Mark Solomon, Peter C. Lovenheim

Laws and Legislation Collection

We conclude from the analysis that the Reporting System, as presently administered, fails to achieve its primary statutory objective: it does not provide APHIS with information sufficient to demonstrate that researchers have used pain-relieving drugs "appropriately" and in accordance with "professionally acceptable standards." The chief reasons for this failing are (1) regulations and guidelines do not define "pain" or "distress," (2) regulations and guidelines do not adequately define "routine procedures," and (3) regulations and guidelines do not require meaningful explanations for the withholding of pain-relieving drugs in procedures acknowledged to cause pain.

The Effect Of Hypercholesterolemia On The Membrane Lipids, 3-0-[¹⁴C] Methylglucose Transport, And 2,3-Diphosphoglycerate Levels In Rat Erythrocytes, Edwin J. Lester Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

An increase in dietary cholesterol and cholic acid will produce hypercholesterolemia in rats. For ten months, two groups of rats (six in each group) were fed commercial rat pellets (control group) or rat pellets plus cholesterol (1%) and cholic acid (0.25%) (experimental group). A blood sample from each animal was divided into three aliquots for three separate experiments on the erythrocytes: a membrane cholesterol and phospholipid determination, a 3-0-[¹⁴C] methylglucose (30MG) transport determination, and a 2,3-diphosphoglycerate (2,3 DPG) determination. The 30MG transport and 2,3 DPG aliquots were each incubated in the same five concentrations of 30MG and glucose …

The Effects Of Orthodontic Repositioning Into A Surgically Expanded Site On Bone Formation And Tooth Mobility In A Baboon, Anthony B. Lier

Loma Linda University Electronic Theses, Dissertations & Projects

For sometime, expansion of the maxillary arch, by means of surgical and mechanical procedures, have been acknowledged with minimal attention directed toward the mandible. In this pilot study a surgical technique was employed in separating the mandible, A-P, at the symphysis. The interosseous void, approximately 10 mm., was then filled with autogenous bone marrow collected from the greater trochanter and the crest of the ilium.

The surgical site was then maintained for a period of eight (8) weeks with the aid of a precast chromium-cobalt splint and titanium mesh placed intradentally and at the symphysis, respectively. Following the stabilization period …

The Role Of The Parotid Salivary Glands In The Regulation Of Insulin Secretion, Kenneth L. Millard

Loma Linda University Electronic Theses, Dissertations & Projects

Stress of handling, partial or complete sialoadenectomy, and the administration of parotid hormone (PH), sodium fluoride (NaF), or guanidino-succinic acid (GSA) were examined for effects on fasting plasma glucose and insulin or effects on intravenous glucose tolerance in male Sprague-Dawley rats. Basal glucose and insulin were substantially elevated as a result of the stress of translocation in large animals, but were not altered in small animals. A rise in plasma insulin occured in control animals 20 minutes following anesthesia and the removal of a blood sample by cardiac puncture but not in animals that had been handled twice daily for …

Ethical Concerns In Primate Use And Husbandry, Ardith A. Eudey

Experimentation Collection

Subsequent to World War II, a dramatic increase occurred in the utilization of nonhuman primates in biomedical and psychological research and industry. At the same time field studies on the ecological and social behavior of natural populations of primates also increased, making possible more realistic assessments of both the behavioral potentiality of primate populations and their conservation status. In spite of the growing body of information indicating the endangered or threatened status of most species, many laboratory workers and planning agencies continue to regard primates as renewable resources, even seeking to bypass protective legislation in habitat countries to obtain them. …

Biomedical Research And Animal Welfare: Traditional Viewpoints And Future Directions, Franklin M. Loew

Experimentation Collection

It has been twenty years since C.P. Snow first presented the concept of "The Two Cultures"; referring to the "culture" of scientists and the "culture" of literary intellectuals (mainly writers), Snow said (1969):

... constantly I felt I was moving among two groups- comparable in intelligence, identical in race, not grossly different in social origin, earning about the same incomes, who had almost ceased to communicate at all, who in intellectual, moral and psychological climate had so little in common ...

In some ways, "Two Cultures" goes far to characterize the current state of affairs surrounding those whose scientific endeavors …

Beyond Conspecifics: Is Brer Rabbit Our Brother?, Gordon Burghardt, Harold Herzog

Experimentation Collection

Today, on many fronts, there is renewed interest in our relationship with nonhuman animals. Many factors have contributed to this concern. Environmental and ecological awareness has drawn public attention to the near extermination of many species and the detrimental effects of pollution, pesticides, and habitat destruction. The inefficiency of transmuting vegetable protein to meat has added to the traditional moral arguments of vegetarians. The widespread questioning of government support for basic research has been intertwined with suspicions about the use and worth of any studies on animals, even those purporting to help understand human medical and behavioral problems. New evidence …

The Effects Of 712-Dimethylbenz (A) Anthracene On Ovarian Steroidogenesis And Uterine Growth In The Mouse, Jeffrey Lynn Clark

Biological Sciences Theses & Dissertations

It has been previously demonstrated that 7,12-dimethylbenz(a) anthracene (DMBA) is an initiator of ovarian granulosa cell tumors. It is also known that DMBA leads to destruction of primordial oocytes, and that tumor induction may involve the interaction of pituitary gonadotropins. However, the effects of DMBA on ovarian steroidogenesis and uterine growth in relation to the tumorigenic process have not been investigated. Therefore, the present study was conducted to examine these parameters using adult C57Bl/6N mice treated in proestrus and diestrus II with 0.1 ml corn oil with or without 1.5 mg DMBA and killed 44 hours later. The uteri and …

Orthognathic Surgical Expansion Procedure Of The Mandible Of Female Baboon Papio Sp?, Willis L. Schlenker

Loma Linda University Electronic Theses, Dissertations & Projects

Expansion of the maxillae is a well recognized procedure in orthodontics. Little is known about Mandibular Expansion. This project is a pure research of the possibility of expanding the mandible laterally at the symphysis on a baboon.

The symphysis was cut A-P and expanded laterally 11 mm, then held and stabilized with a titanium mesh work screwed to the inferior border of the mandible. The defect area was filled with a bone graft of marrow and cancellous bone fragments taken from the greater trochanter and the crest of the ilium. New bone did fill in the defect and the expansion …

An Ultra-Violet Light Microscopic Study Of The Hydroxylapatite Ceramic-Bone Interface Phenomena, Leonard W. Pierce

Loma Linda University Electronic Theses, Dissertations & Projects

Preparations used in this study were obtained from an investigation which had undertaken the following:

Durapatite, a hydroxylapatite ceramic was implanted in 24 mature Beagle dogs to study its maintenance effect on the alveolar bone of the mandible.

After dividing the dogs into two groups, a fresh socket and a healed socket group, the posterior teeth were removed and alveolectomies were performed. Eight ceramic implants were then placed in the right mandible of each animal in each group. The healed group received the implants five weeks after tooth removal and the fresh socket group received implants immediately after extraction.

The …

The Rate Of The Healing Process In Venous Thrombi Produced By A Sclerosing Agent With Special Attention To Endothelial Lining, Kiyoshi Tanaka

Loma Linda University Electronic Theses, Dissertations & Projects

Despite extensive clinical and experimental investigations of venous thrombosis, relatively little is known about the early phases of healing of the venous thrombus. Soon after its discovery heparin has become the most popular agent for the prevention and treatment of venous thrombosis, but there are a number of unanswered questions regarding the most satisfactory treatment for venous thrombosis.

One area of controversy has been the duration of anticoagulation therapy for acute venous thrombosis. If the anticoagulant is discontinued too soon, the thrombus may continue to propagate. Prolonged anticoagulant therapy exposes the patient to the risk of serious hemorrhage. The objective …

Live Animals In Car Crash Studies, Nancy Heneson

Experimentation Collection

The scientific rationale for using live animals in car crash studies proceeds from the argument that comparative biomedical and biomechanical data are needed to develop an instrumented dummy, or anthropomorphic test device, which will provide reliable, reproducible information for designing safe cars. The animal studies are thus not really ends in themselves, i.e., they do not supply data which can be readily applied to real situations. Instead, they contribute to a pool of information which is supposed to lead to the perfecting of an experimental subject (the instrumented dummy) which will eventually render the further use of Iive animals unnecessary.