Research Methods in Life Sciences Commons^™

Founders Of Plant Ecology: Frederic And Edith Clements, Jon H. Oberg

University of Nebraska State Museum: Staff and Affiliates, Publications

Nineteenth-century students of Charles Bessey at the University of Nebraska, Frederic Clements and Edith Schwartz received doctorates in botany, married, and went on to become founders of the discipline of plant ecology. They tested and taught their theory of plant succession, known as Clementsian ecology, for nearly four decades at their Alpine laboratory in Colorado. Their leadership and influence at the Carnegie Institution was world-wide and attracted followers from several other disciplines. They advocated land use measures to combat the Dust Bowl in the 1930s. Clementsian ecology is still recognized as a paradigm against which other theories of nature are …

The Influence Of Location, Positioning, And Seasonality On Feeding Behavior Of The Sydney Oyster (Saccostrea Glomerata) In New South Wales, Australia, Nathaniel Hess

Independent Study Project (ISP) Collection

Oysters are hard shelled bivalves that aggregate over time to create structures in estuarine systems, known as oyster reefs. These reefs are important for the promotion of estuarine biodiversity by supporting many species of fish, invertebrates, and plants. They also act as a key contributor to water health by using active suspension feeding mechanisms and selective feeding to remove nutrients and water borne pollutants from estuarine systems. They have been touted as possible bio-remediators. They also effect rates of sedimentation in estuaries.

Oyster reefs have historically been threatened by anthropogenic influences such as overharvesting, destructive fishing practices, water pollution, CO2 …

36 Years After The Species' Mass-Mortality: Diadema Antillarum Test Sizes, Population Densities, And Substrate Preferences In Three Guna Yala Reefs Near Wichub Wala Island, Bimini Horstmann

Independent Study Project (ISP) Collection

In January 1983, a waterborne pathogen beginning near the Atlantic Panama Canal entrance caused history's largest recorded marine animal die-off, wiping out 95-99% of Diadema antillarum populations throughout the Caribbean. D. antillarum, the long-spined sea urchin, is a keystone herbivore in coral reefs and its decreased densities have caused many reefs to suffer macroalgal phase shifts. Modest recovery of this species has been documented in some Caribbean locations, but reefs in Guna Yala, Panama continued to experience population decline. This study investigates density, test size, and substrate preferences of D. antillarum in three shallow coral reef areas to update the …

Andean Condor Cliff And Parental Care Behavior: A Behavioral Study Of A Chick And His Free-Living Parents On The Peñón Del Isco, Ecuador, Ellie Cohn

Independent Study Project (ISP) Collection

Andean condors (Vultur gryphus) are a Near Threatened species of New World Vulture found throughout the Andes region of South America. Their population has been significantly diminished in the country of Ecuador due to threats primarily related to habitat loss and persecution by humans. High chick and juvenile mortality also threaten populations and as a result, investigation of wild Andean condors’ cliff behavior and parental care are necessary in order to develop informed and effective conservation management plans. A 22-day observational study was conducted, monitoring a known breeding pair of Andean condors and their approximately three-month-old male chick on the …

Effectiveness Of Different Agricultural Management Styles As Insect Biological Corridors: A Comparison Of Insect Populations In Fragmented Chocó Cloud Forest, Ecuador, Tara M. Krantz

Independent Study Project (ISP) Collection

Insects are part of the most diverse class of animals on the planet and are essential to various ecological functions such as pollination, nutrient cycling, providing a food source for other taxa, and more. The diversity and ecological services of insects are necessary to the operation of agriculture because of pest control and pollination of crops. However, the diversity of insects is severely reduced due to fragmentation. It is currently not well understood if certain types of agriculture can lessen the impact of fragmentation on natural and crop-based insect communities. In this study, insect populations in four different agricultural management …

Cambios En El Cronograma De Temporadas Reproductivas De Pingüinos De Magallanes (Spheniscus Magellanicus): Relaciones Entre Eventos Fenológicos Y Variables Climáticas, Grace Neumiller

Independent Study Project (ISP) Collection

Estudios a largo plazo han demostrado que hay fluctuaciones a lo largo de la fenología de la vida silvestre a causa de los cambios climáticos (Black, 2016). Los pingüinos de Magallanes (Spheniscus magellanicus) exhiben cambios en el cronograma de los eventos fenológicos de sus temporadas reproductivas a causa de variables oceanográficas y climáticas de su ambiente. A partir de fotos de cámaras trampas, este trabajo describe y analice algunos eventos en la fenología de los pingüinos de Magallanes en Isla Martillo, Canal Beagle, Tierra del Fuego, Argentina: el inicio de la temporada reproductiva, el periodo de muda de pichones, y …

Araneae Biodiversity In The Amazonian Foothills: An Analysis Of Family And Guild Presence Across Habitat Types, Rae Lewark

Independent Study Project (ISP) Collection

The activity of the order Araneae within ecosystems is often considered an ecological mystery. Considered top-level trophic predators, spiders act as regulators of insect populations and have influence upon the foundation of the trophic web. In the Ecuadorian Amazonian foothills, it is important to address the lack of knowledge of the biodiversity of spiders in the region. Conducting biodiversity studies across land use gradients can begin to characterize anthropogenic impacts on neotropical spider species. This study quantifies the araneae biodiversity in the transition between the Cloud and Amazon forests in Ecuador. Populations in primary forest, secondary forest, cultivated fields and …

Mammals Of The Northern Andes: An Analysis Of Camera Trap Data And Observation In Angochagua, Ecuador, Risa Berman

Independent Study Project (ISP) Collection

The high Andes of Ecuador are home to a diverse community of mammals, many of which are threatened. These include the Andean bear, Andean fox, puma, and mountain tapir. This study used camera traps, direct observation, and indirect observation over three weeks to monitor the wildlife in the mountains of the Parroquia of Angochagua. The five species observed during this period were Andean bear, Andean fox, dog, mountain paca, and mouse. While this short study did not completely survey the mammalian community of the area, it confirmed the presence, diet, sociality, temporal habits, elevational range, and habitat preferences of the …

Spatial And Temporal Variability In Post-Larval Yellow Perch Density, Steve Hauschildt

Biology Graduate Theses

Yellow Perch Perca flavescens are important as forage for other species and for sport fishing in many northern lakes. However, estimating post-larval Yellow Perch populations can be difficult because of many environmental factors that cause unexplained variation. The objective of this study was to help reduce unexplained variation by determining a post-larval Yellow Perch population density estimate on Blackduck Lake, MN, provide guidance to help determine the number of trawls required for trawling-based recruitment indices to achieve varying levels of precision, and test for the effects of wind speed and direction on post-larval Yellow Perch spatial variability. This study estimated …

A Prairie And Its People: Conflict And Cooperation. E. Frank Schramm, Joseph E.A. Alexis, The Amos Eager Family, The University Of Nebraska And Other Notable Owners Of Nine Mile Prairie And Its Environs, Jon H. Oberg

University of Nebraska State Museum: Staff and Affiliates, Publications

This study looks at the social history of individuals connected to a particular geographic quadrant -- Section 1 of Middle Creek precinct, Lancaster County, Nebraska. This an area famous for its natural history, as it is the home of Nine Mile Prairie. But the property owners in this section have a colorful history that encompasses the fields of sports, politics, academics, foreign wars, and crime. The study looks primarily at E. Frank Schramm, Joseph E.A. Alexis, and the Amos Eager family, who with other owners contributed importantly to the history and development of the City of Lincoln and the State …

Population Health Of Spotted Salamanders (Ambystoma Maculatum) In Created Vernal Pools: An Integrative Approach, Alice R. Millikin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Habitat creation is an important tool for conservation to counteract habitat loss and degradation. Vernal pools are susceptible to destruction due to limited detection, protection, and regulation. These wetlands provide fishless breeding habitat for many amphibian species including spotted salamanders (Ambystoma maculatum) in eastern North America. Determining whether created vernal pool habitat is successful is often determined by demographic data of colonizing populations. I suggest that hormone levels, population genetics, and disease prevalence can improve our understanding of population health in created habitat. The goal of this dissertation was to assess the health of spotted salamander larvae in …

Growth Of Pelagic Age-0 Yellow Perch In Relation To Hatch Dates And Water Temperature In North-Central Minnesota Lakes, Ethan Karppinen

Biology Graduate Theses

Exposure to consistent water warming rates and access to abundant populations of edible sized zooplankton affects growth and increases the probability of survival for newly hatched Yellow Perch Perca flavescens. Seven lakes with varying physical characteristics were selected in north-central Minnesota to study abiotic (water temperature) and biotic (zooplankton community and fish growth) progressions during the spring and early summer (April-June). Mean water temperatures from ice-out to 28 June (11.98-14.62 °C) had significant relationships with mean depth (P < 0.01, R? = 0.85), max depth (P < 0.01, R? = 0.73), and total volume (P < 0.01, R2 = 0.54). Hatch periods ranged from 13 to 19 days in 2015 and 13 to 25 days in 2016. Cumulative degree-days (CDD) were used to predict Yellow Perch hatch periods and explain growth. CDD predicted 21-81% of hatch periods in 2015 and 11-50% in 2016. Mean predicted total lengths at 28 June were 19.65-30.41 mm in 2015 and 20.88-28.54 mm in 2016. There was a significant relationship between CDD and predicted total length (R? = 0.62, P < 0.01) to explain differences in Yellow Perch total length while inhabiting the pelagic zone. Observed gape measurements were highly correlated with predicted gapes from the Schael formula (R? = 0.89, P < 0.01) that was used to assess the availability of edible sized zooplankton for age-0 Yellow Perch in each lake. No predator-prey mismatches were found and earlier hatch dates produced age-0 Yellow Perch with greater predicted total lengths in late June on the majority of lakes. This suggests that earlier hatched Yellow perch have an advantage over later hatched Yellow Perch in attaining greater lengths by late June. Our study provides information on a variety of physical and biological progressions in lakes, which may be used for fisheries management practices in the future.

Population Characteristics Of A Closed Northern Pike (Esox Lucius) Catch-And-Release Population In Minnesota, John Kempe

Biology Graduate Theses

Literature regarding catch-and-release populations of Northern Pike are rare and provide a unique perspective on their population dynamics. Shingobee Lake is a 64- ha mesotrophic lake with a maximum depth of 12.2 m located in northern Minnesota. Northern Pike fishing has been catch-and-release only since 2005, and there are no public accesses on the lake. Annual ice-out trap-netting occurred from 2009-2017, and a sizable amount of the Northern Pike recaptured via trapnet or angling had been previously tagged (37-59%). The lack of exploitation and high tagging percentage provided a unique opportunity to study the population dynamics of Northern Pike. Shinogbee’s …

Estimating Exploitation Rates In The Alabama Red Snapper Fishery Using A High-Reward Tag–Recapture Approach, Dana K. Sackett, Mattgew Catalano, J. Marcus Drymon, Sean P. Powers, Mark Albins

University Faculty and Staff Publications

Accurate estimates of exploitation are essential to managing an exploited fishery. However, these estimates are often dependent on the area and vulnerable sizes of fish considered in a study. High-reward tagging studies offer a simple and direct approach to estimating exploitation rates at these various scales and in examining how model parameters impact exploitation rate estimates. These methods can ultimately provide a better understanding of the spatial dynamics of exploitation at smaller local and regional scales within a fishery—a measure often needed for more site-attached species, such as the Red Snapper Lutjanus campechanus. We used this approach to tag 724 …

Compression And Relaxation Of Fishing Effort In Response To Changes In Length Of Fishing Season For Red Snapper (Lutjanus Campechanus) In The Northern Gulf Of Mexico, Sean P. Powers, Kevin Anson

University Faculty and Staff Publications

A standard method used by fisheries managers to decrease catch and effort is to shorten the length of a fishery; however, data on recreational angler response to this simple approach are surprisingly lacking. We assessed the effect of variable season length on daily fishing effort, measured by using numbers of boat launches per day, anglers per boat, and anglers per day from video observations, in the recreational sector of the federal fishery for red snapper (Lutjanus campechanus) in coastal Alabama. From 2012 through 2017, season length fluctuated from 3 to 40 d. Daily effort, measured by using mean number of …

Bacteria Mitigation In Sponge Mariculture, Jambiani Zanzibar, Claire Johnston

Independent Study Project (ISP) Collection

The declining seaweed industry coupled with efforts by the NGO Marine Cultures have led to the establishment of sponge mariculture in Jambiani, Zanzibar. However, growing cyanobacteria levels have substantially increased sponge mortality rates. In order to determine successful cyanobacteria mitigation treatments, six populations of farm ropes, support lines, and sponges were (a) untreated, (b) manually cleaned or submerged in solutions of (c) 2% hydrogen peroxide, (d) 4% hydrogen peroxide, (e) 50 g/L salt, or (f) 70 g/L salt. No conclusions were drawn from rope treatments, no techniques were effective for support line treatments, and both concentrations of hydrogen peroxide were …

Rehabilitation Of An Injured Golden Eagle (Aquila Chrysaetos): A Case Study, Fida Muhammad Khan, Fakhar-I- Abbas, Abu Ul Hassan Faiz

Journal of Bioresource Management

Habitat destruction and anthropogenic activities cause wild birds to migrate towards urban areas in search of food and sometimes nesting in high-roof buildings, where they are caught by local people using different techniques and are further used for hunting or recreational purposes. An injured Golden Eagle (Aquila chrysaetos) was found entangled in bushes near the vicinity of Balkasar Research Complex, Chakwal, Pakistan. After complete physical examination, a wound on the right wing and closed leg fracture was diagnosed. The successful treatment was done by anti-parasitic and anti-bacterial drugs with careful monitoring. Splint was adjusted after aligning the fractured …

Estimating Seasonal Variation In Condition Of Yellow Perch, Emily Anne Powers

Biology Graduate Theses

Research has shown that Yellow Perch Perca flavescens, found throughout lakes and streams of North America, are good indicators of environmental health. Due to the correlation between fish condition and ecosystem health it is essential to reliably estimate condition to better manage our fisheries. Therefore, the study objectives were to measure the seasonal changes in adult Yellow Perch percent dry weight (PDW), and compare the reliability of bioelectrical impedance analysis (BIA) and morphometric-based estimates of condition. The seasonal fluctuations in average monthly PDW were measured over a two- year period and were sinusoidal in shape with peaks in August 2015 …

Survey Results: Complexities And Overlaps In Existing Citizen Science Mosquito Projects, Yujia He, Elizabeth Tyson

Patterson School of Diplomacy and International Commerce Faculty Publications

Currently, multiple projects worldwide engage the public in the scientific process of learning about mosquito biodiversity or monitoring mosquitoes that carry diseases like Dengue, Chikungunya, Zika and Malaria. These projects are executed at different invasion-stages and different scenarios of epidemiological risk based on their country of origin. Through surveying 12 international research teams, this brief report illuminates the differences and the overlaps in the protocols and tools used by existing citizen science mosquito monitoring projects. The analysis covers project goals, requirements and support for participants, policy impact, digital tools and data privacy.

Marine Ecoregion And Deepwater Horizon Oil Spill Affect Recruitment And Population Structure Of A Salt Marsh Snail, Steven C. Pennings, Scott Zengel, Jacob Oehrig, Merryl Alber, T. Dale Bishop, Donald R. Deis, Donna Devlin, A. Randall Hughes, John J. Hutchens, Jr., Whitney M. Kiehn, Caroline R. Mcfarlin, Clay L. Montague, Sean P. Powers, C. Edward Proffitt, Nicholle Rutherford, Camille L. Stagg, Keith Walters

University Faculty and Staff Publications

Marine species with planktonic larvae often have high spatial and temporal variation in recruitment that leads to subsequent variation in the ecology of benthic adults. Using a combination of published and unpublished data, we compared the population structure of the salt marsh snail, Littoraria irrorata, between the South Atlantic Bight and the Gulf Coast of the United States to infer geographic differences in recruitment and to test the hypothesis that the Deepwater Horizon oil spill led to widespread recruitment failure of L. irrorata in Louisiana in 2010. Size-frequency distributions in both ecoregions were bimodal, with troughs in the distributions consistent …

First Record Of The Beetle Family Throscidae (Insecta: Coleoptera), A New Species Of I>Aulonothroscus Horn, And New Species Records To The Fauna Of Peru / Primer Registro De La Familia De Escarabajos Throscidae (Insecta: Coleoptera), Una Nueva Especie De Aulonothroscus Horn Y Tres Nuevos Registros De Especies Para La Fauna De Perú, Paul J. Johnson, Caroline S. Chaboo

University of Nebraska State Museum: Staff and Affiliates, Publications

The beetle family Throscidae is documented from Peru for the first time, based on specimens collected in the regions of Cajamarca, Junín, Loreto and Madre de Dios. Aulonothroscus tambopata new species is descri­bed from Madre de Dios. Also, three additional species of Aulonothroscus are reported from Peru for the first time – A. alvarengai Cobos new country record, A. freudi Cobos new country record, and A. oculatissimus Cobos new country record. A key separating these four species is given. This report is part of the “Beetles of Peru” project.

Se documenta por primera vez la familia de …

The Role Of Citizens In Detecting And Responding To A Rapid Marine Invasion, Steven B. Scyphers, Sean P. Powers, J. Lad Akins, J. Marcus Drymon, Charles W. Martin, Zeb H. Schobernd, Pamela J. Schofield, Robert L. Shipp, Theodore S. Switzer

University Faculty and Staff Publications

Documenting and responding to species invasions requires innovative strategies that account for ecological and societal complexities. We used the recent expansion of Indo-Pacific lionfish (Pterois volitans/miles) throughout northern Gulf of Mexico coastal waters to evaluate the role of stakeholders in documenting and responding to a rapid marine invasion. We coupled an online survey of spearfishers and citizen science monitoring programs with traditional fishery-independent data sources and found that citizen observations documented lionfish 1–2 years earlier and more frequently than traditional reef fish monitoring programs. Citizen observations first documented lionfish in 2010 followed by rapid expansion and proliferation in …

Female Breakfast Skippers Display A Disrupted Cortisol Rhythm And Levated Blood Pressure, Megan Witbracht, Nancy L. Keim, Shavawn Forester, Adrianne Widaman, Kevin D. Laugero

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Chronic stress and over-activity in the hypothalamic–pituitary–adrenal (HPA) axis may link breakfast skipping and poor cardiometabolic health. Missing the first major meal of the day in rodents prolongs elevated circulating corticosterone at a time when it's normally decreasing. To extend these findings to humans, we hypothesized that habitual breakfast skippers would display a similar pattern of circulating cortisol and alterations in meal and stress-induced cortisol reactions. Normal weight to obese women aged 18–45 years old who were strictly defined as either breakfast skippers (n = 30) or breakfast eaters (n = 35) were invited to participate in our …

Integrating Societal Perspectives And Values For Improved Stewardship Of A Coastal Ecosystem Engineer, Steven B. Scyphers, J. Steven Picou, Robert D. Brumbaugh, Sean P. Powers

University Faculty and Staff Publications

Oyster reefs provide coastal societies with a vast array of ecosystem services, but are also destructively harvested as an economically and culturally important fishery resource, exemplifying a complex social-ecological system (SES). Historically, societal demand for oysters has led to destructive and unsustainable levels of harvest, which coupled with multiple other stressors has placed oyster reefs among the most globally imperiled coastal habitats. However, more recent studies have demonstrated that large-scale restoration is possible and that healthy oyster populations can be sustained with effective governance and stewardship. However, both of these require significant societal support or financial investment. In our study, …

Changing Body Condition In Walleye With Reference To Ideal Sample Size Requirements, Kevin Longtin

Journal of Earth and Life Science

The objective of this study was to determine if walleye Sander vitreus percent dry weight, a measure of condition, differed among summer months of 2014 on Maple Lake, Minnesota. A second objective was to determine if there was a relationship between condition and body temperature, total length, or wet weight. Between three and six walleyes were collected per month by hook and line methods. All fish had internal body temperatures taken at the time of capture and were oven dried in the laboratory at 80 °C in order to measure percent dry weight. There was no relationship between condition and …

Validation Of Pcr-Based Assays And Laboratory Accreditation For Environmental Detection Of Aquatic Invasive Species, Invasive Species Advisory Committee

National Invasive Species Council

This white paper provides:

a) Background information on the use, accuracy and reliability of PCR-based assays such as environmentally sampled DNA (eDNA) for early detection of aquatic invasive species (AIS) and;

b) Recommendations for establishing a system for validating assays and accrediting laboratories that report on the presence or absence of AIS.

This white paper was developed by the members of ISAC and discusses the need for developing validation requirements for Polymerase Chain Reaction (PCR) and other DNA-based molecular assays that are increasingly being used to detect AIS. It does not provide a simplified checklist for evaluation of their ability …

Age Composition And Distribution Of Red Drum (Sciaenops Ocellatus) In Offshore Waters Of The North Central Gulf Of Mexico: An Evaluation Of A Stock Under A Federal Harvest Moratorium, Sean P. Powers, Crystal Hightower, J. Marcus Drymon, Matthew W. Johnson

University Faculty and Staff Publications

Because of a lack of fishery- dependent data, assessment of the recovery of fish stocks that undergo the most aggressive form of management, namely harvest moratoriums, remains a challenge. Large schools of red drum (Sclaenops ocellatus) were common along the northern Gulf of Mexico until the late 1980s when increased fishing effort quickly depleted the stock. After 24 years of harvest moratorium on red drum in federal waters, the stock is in need of reassessment; however, fishery dependent data are not available in federal waters and fishery-independent data are limited. We document the distribution, age composition, growth, and condition of …

Can R&D Investment Offset The Negative Impact Of Climate Change On Agricultural Productivity?, Nazrul Islam, Ruhul A. Salim

Climate Science Published Reports

Productivity growth in agriculture helps maintain the nation's economic prosperity. However, long-run productivity growth in agriculture faces several challenges such as the potential worsening of climate due to global warming and the loss of productive land due to land use competition, salinity, wind erosion and soil acidity. Strategic policy making and appropriate funding for sustained productivity growth in agriculture is therefore crucial.

Research and development (R&D) to drive innovation is an acknowledged main source of productivity growth. The development and application of technologies generated through R&D boosts agriculture's productivity. Agricultural R&D activities in Australia and in most other developed economies …

Mammals Of Nebraska: Checklist, Key, And Bibliography, Hugh H. Genoways, Justin D. Hoffman, Patricia Freeman, Kenneth N. Geluso, Russell A. Benedict, Jeffrey J Huebschman

University of Nebraska State Museum: Staff and Affiliates, Publications

The Recent mammalian fauna of Nebraska is composed of 89 native species, 8 domestic species that can have feral populations, and 4 wild species introduced by humans, but not by intentional action. Thus, the included checklist and dichotomous key contain 101 species of mammals. Of the native species, 36 are rodents, 20 carnivores, 13 bats, 7 shrews and mole, 7 even-toed ungulates, 4 rabbits and hares, 1 armadillo, and 1 opossum. Another 8 species are identified as potentially occurring in peripheral areas of Nebraska. The bibliography includes 1693 entries that each contain data concerning one or more species of mammals …

Agribusiness Sheep Updates - 2004 Part 3, Ken Hart, Keith Croker, Rob Davidson, Mario D'Antuono, Evan Burt, Tanya Kilminster, Diana Fedorenko, Darryl Mcclements, Robert Beard, Sandy Turton, Martin Atwell, Robert Beard

Sheep Updates

Posters Can Merino Ewes be Teased to Synchronise Oestrus for a Summer/Autumn Mating? Ken Hart, Keith Croker, Department of Agriculture Western Australia, Rob Davidson, School of Animal Biology, University of Western Australia. Mario D'Antuono, Department of Agriculture Western Australia Damara Sheep: Now Looking Domestic Evan Burt and Tanya Kilminster, Department of Agriculture Western Australia Production and Water Use of Lucerne and French Serradella Under Three Sowing Rates Diana Fedorenko1,4, Darryl McClements1,4 , Robert Beard3,4 1Department of Agriculture, 3 Farmer, Meckering, 4CRC for Plant-based Management of Dryland Salinity. E-SHEEP - Individual Animal Management is here Sandy Turton & Martin Atwell, Department …