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Articles 91 - 120 of 301
Full-Text Articles in Research Methods in Life Sciences
Characteristics Of Athletic Trainers That Are Satisfied And Dissatisfied With Their Compensation, K Ef Mair, J P. Young, L E. Eberman
Characteristics Of Athletic Trainers That Are Satisfied And Dissatisfied With Their Compensation, K Ef Mair, J P. Young, L E. Eberman
Clinical Practice in Athletic Training
Introduction: Job satisfaction among athletic trainers (ATs), particularly in relation to compensation, is a critical factor influencing retention and career fulfillment. Examining the factors linked to earnings satisfaction may offer insight into how workplace conditions can be improved to better support ATs. The purpose of our study was to investigate the attributes that distinguish ATs content with their compensation from those who are dissatisfied. Methods: We used a cross-sectional, web-based survey to gather AT compensation data, of which 1,887 participants responded to a question regarding their satisfaction with compensation. Respondents were divided into two groups based on their satisfaction with …
The Effects Of A Joint Reduction Trainer On Confidence Levels In Professional Athletic Training Students, T Franklin, S Hacherl
The Effects Of A Joint Reduction Trainer On Confidence Levels In Professional Athletic Training Students, T Franklin, S Hacherl
Clinical Practice in Athletic Training
Introduction: Dislocations are common injuries managed by athletic trainers, yet athletic training students are unlikely to practice reduction techniques during clinical experiences. Current standards set by the Commission on Accreditation of Athletic Training Education require accredited programs to prepare students to manage patients with dislocations, including joint reductions. Research indicates the use of simulation techniques, such as joint reduction simulators, in education can fill clinical experience gaps and improve student confidence and skills. Shoulder dislocations make up nearly 50% of dislocations with several reduction techniques identified in the current literature. The use of a joint reduction simulator may provide athletic …
Systematic Review Of Immunohistochemistry Protocols, Arpitha Parthasarathy
Systematic Review Of Immunohistochemistry Protocols, Arpitha Parthasarathy
Pitzer Senior Theses
Immunohistochemistry (IHC) is an essential technique in biological research that allows for the visualization and localization of proteins within tissue samples. This systematic review analyzes the most effective, efficient and reproducible protocols for IHC. It focuses on three critical steps: freezing, cryosectioning, and staining of skeletal muscle tissues in mice models. A total of 41, 44, and 48 articles on freezing, cryosectioning, and staining techniques, respectively, were analyzed, from reputable sources in biology like PubMed and Web of Science. Results showed the following. For freezing, the use of isopentane combined with liquid nitrogen appeared to be the best method, minimizing …
Training Module And Assessment Strategy For Light Microscopy Skills Training In A Research Environment, Kristopher E. Kubow, Joseph A. Harsh
Training Module And Assessment Strategy For Light Microscopy Skills Training In A Research Environment, Kristopher E. Kubow, Joseph A. Harsh
Faculty Scholarship
Training in specialized techniques and instrumentation is an important component of undergraduate and graduate education. While such training can occur in formal lab courses, it also occurs as students engage in research. Although training in a research lab setting is typically informal, implementing evidence-based practices from the classroom would be beneficial, particularly for learning complex techniques and instruments, for example, light microscopy. Using advanced microscopes for research is more complicated than using teaching microscopes in a course. In addition, microscopes are easy to misuse because they will generally always produce an image even if that image is suboptimal, misleading, or …
Caspases Protect Against Hyperglycemia Induced Necroptosis-Like Cell Death In Brain Endothelial Cells, Aleena S. Mathew
Caspases Protect Against Hyperglycemia Induced Necroptosis-Like Cell Death In Brain Endothelial Cells, Aleena S. Mathew
Theses and Dissertations
Acute diabetes characterized by high spikes in blood glucose levels in association with severe hyperglycemia can lead to an increased risk of ischemic stroke in the brain. To better understand high blood glucose levels, hyperglycemia, in the brain we explored its effects on endothelial cells. Endothelial cells line the inner walls of blood vessels and are directly impacted in hyperglycemia. Brain endothelial cells are a major component of the blood brain barrier (BBB) which tightly regulates the brain’s microenvironment. The type of cell death such as apoptosis, caspase dependent, or necroptosis, caspase independent, can exacerbate diabetes associated complications. There is …
Effects Of Diet Composition On Physical Parameters In Danio Rerio, Isabella Hemphill
Effects Of Diet Composition On Physical Parameters In Danio Rerio, Isabella Hemphill
Williams Honors College, Honors Research Projects
With the use of zebrafish as a common model organism for human health, the reduction of confounding variables in their care is key. Many commercialized zebrafish diets are available for purchase, but each one consists of a different ratio of these crucial macronutrients. This study aimed to determine the effects long-term diets high in one macronutrient would have on the morphometrics, heart, liver, and protein content of zebrafish. Four diets were fed over a period of almost two years- high lipid, high carb, high protein, and a control diet. The control diet was the standard feed used in this lab, …
Impact Of Tetrahydrocannabinol (Thc) On Daphnia Magna Heart Rate, Ashley Cosgrave
Impact Of Tetrahydrocannabinol (Thc) On Daphnia Magna Heart Rate, Ashley Cosgrave
Williams Honors College, Honors Research Projects
Marijuana (Cannabis) is currently federally illegal within the United States and classified as a Schedule I drug. This leaves a large research gap on the impacts of Tetrahydrocannabinol (THC) use. Daphnia magna are frequently used as a model organism for the human heart and toxicology screenings due to their myogenic heart, similarities in physiological pathways, and sensitivity to toxins. As such, in order to bridge the research gap and uncover potential use of Daphnia magna as a model organism for humans in regard to impact on heart rate, we hypothesized that if Daphnia magna possess CB1 receptors or an equivalent …
Transcriptome-Wide Gene Expression Analysis Of Skeletal Muscles Of Duchenne Muscular Dystrophy Mouse Models And Patients, Olivia N. Ransiear
Transcriptome-Wide Gene Expression Analysis Of Skeletal Muscles Of Duchenne Muscular Dystrophy Mouse Models And Patients, Olivia N. Ransiear
Electronic Theses & Dissertations (2024 - present)
Duchenne Muscular Dystrophy (DMD) is an X-linked recessive muscle degenerative disease that frequently proves fatal by age 30. It primarily affects adolescent males with symptoms appearing as early as age 6. Patients quickly lose their ability to walk due to muscle weakness and are confined to wheelchairs for the remainder of their lives. DMD is caused by a mutation in the dystrophin gene. Without dystrophin, mechanical stress, injury, or shock takes a drastic toll on muscle tissue and muscle stem cells, rendering them unable to regenerate or be repaired. This results in a rapid loss of muscular strength and muscle …
Timing Of Migration And Seasonal Movements Of Arctic Grayling (Thymallus Arcticus) In Relation To Water Temperature In The Kakisa River And Upper Mackenzie River, Nwt: Results From An Acoustic Telemetry Study., Levi Snook
Theses and Dissertations (Comprehensive)
Arctic grayling (Thymallus arcticus) are a cold-water salmonid that inhabit waterbodies throughout the circumpolar regions of the northern hemisphere. In the last decade, Ka'a'gee Tu First Nation members as well as local sport fishers, have observed lower catches of Arctic grayling during their annual spawning run in the Kakisa River, NWT. Many potential causes of this decline have been hypothesized including climate change and over harvesting. Outside of the general annual spawning activity in the headwaters of the Kakisa River, the ecology of the population, including post-spawn habitat use and migratory patterns, remained understudied. To address these knowledge gaps, acoustic …
Dog Sniffing Biomechanic Responses In An Odor Detection Test Of Odorants With Differing Physical Properties, Lydia Burnett, Nicholas Hebdon, Pete A. Stevens, Monica Moljo, Lindsay D. Waldrop, Lauryn Degreeff
Dog Sniffing Biomechanic Responses In An Odor Detection Test Of Odorants With Differing Physical Properties, Lydia Burnett, Nicholas Hebdon, Pete A. Stevens, Monica Moljo, Lindsay D. Waldrop, Lauryn Degreeff
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Dogs are utilized in forensic science for their extensive scent detection capabilities. They are often considered the “gold standard” in field detection for targets such as illicit drugs and explosives. Despite their prevalence in the field, relatively little is known about how dogs interact with and transport volatile organic compounds through their olfactory system. In this study, two groups of dogs were utilized – Sport detection dogs (n=19) that participate in the National Association of Canine Scent Work and have achieved advanced standing through training and successful search competitions and law enforcement explosive detection dogs (n=8) which were included for …
Limits Of Longwood's Electron Microscope: A Study In Biological Imaging, Rachel Danzitz, Bjoern Ch. Ludwar
Limits Of Longwood's Electron Microscope: A Study In Biological Imaging, Rachel Danzitz, Bjoern Ch. Ludwar
Fall Showcase for Research and Creative Inquiry
This study evaluates the imaging capabilities and limitations of the Hitachi TM4000II Plus Scanning Electron Microscope (SEM) and its Scanning Transmission adapter in biological imaging. A variety of specimens, including protozoans (e.g., Volvox and nematodes), bacteria (e.g., Magnetospirillum gryphiswaldense, Bacillus cereus, and Escherichia coli), tissue samples, and blood, were analyzed. While the SEM effectively imaged protozoans and bacteria, its ability to visualize viruses was deemed impractical. Despite claims of magnification up to 100,000x, clarity significantly decreased beyond 5,000x. The transmission adapter proved functional but challenging to operate consistently. The study highlights the microscope's limitations in producing high-clarity, …
Oxidative Stress Responses In Arabidopsis Thaliana Plants To Uv-B Irradiation, Bolaji Olalere
Oxidative Stress Responses In Arabidopsis Thaliana Plants To Uv-B Irradiation, Bolaji Olalere
Theses
The depletion of the stratospheric ozone layer has led to increased levels of harmful UV-B radiation reaching the Earth's surface, posing significant challenges for plant health and development. This study investigates the oxidative stress responses in Arabidopsis thaliana when exposed to UV-B irradiation. Arabidopsis thaliana, a well-established model organism, is particularly sensitive to UV-B, making it ideal for understanding the effects of UV-B-induced oxidative stress. UV-B radiation, a mutagenic component of sunlight, disrupts key metabolic, molecular, and developmental processes, leading to DNA damage, impaired metabolism, and morphological changes. This research aims to explore how UV-B exposure affects the activities of …
Growth And Mobility Of Physella Acuta Under Individual And Combined Acetaminophen And Salinity Exposure, Rhea Wilder, Jason Doll, Daniel Elias
Growth And Mobility Of Physella Acuta Under Individual And Combined Acetaminophen And Salinity Exposure, Rhea Wilder, Jason Doll, Daniel Elias
Journal of the South Carolina Academy of Science
Global climate change, driven by rapid population growth and anthropogenic activities, presents an urgent challenge to aquatic ecosystems. Salinity intrusion threatens water quality and availability for human and wildlife populations. At the same time, due to the demand for pharmaceuticals, these pollutants are increasingly detected in freshwater environments, with acetaminophen being one of the most prevalent. This research analyzed the individual and combined effects of salinity (2.5 g/L) and acetaminophen (488 μg/L) on Physella acuta. Overall, we observed reduced snail movement under elevated salinity and no effects on growth or movement with acetaminophen alone or in combination with salinity. …
Louisiana Graduate Network In Applied Evolution (Lagniappe): 2023-2024 End-Year Report, Kristina Little, Samuel B. Robison, Bret Blackmon, Andrew R. Burns
Louisiana Graduate Network In Applied Evolution (Lagniappe): 2023-2024 End-Year Report, Kristina Little, Samuel B. Robison, Bret Blackmon, Andrew R. Burns
Reports
The Louisiana Graduate Network in Applied Evolution (hereinafter; LAGNiAppE) was funded by a grant awarded through the National Science Foundation (NSF 22-506), as part of the Research and Mentoring for Postbaccalaureates in Biological Sciences (RaMP) grant program. The LAGNiAppE program provides training and mentorship to cohorts of recent college graduates from underrepresented groups interested in advancing their knowledge and research skills in evolutionary biology. Social Research and Evaluation Center (SREC) at Louisiana State University conducted the evaluation of LAGNiAppE, assessing program implementation and impact. The evaluation team at SREC employed a mixed methods design that included online surveys for scholars …
Standardized Patient Evaluation Tool: A Valid Measure Of The Core Competencies, Lindsey E. Eberman, Jessica Edler Nye
Standardized Patient Evaluation Tool: A Valid Measure Of The Core Competencies, Lindsey E. Eberman, Jessica Edler Nye
Clinical Practice in Athletic Training
No abstract provided.
The Value Of Using Validated Competency-Based Assessments Across The Career Span Of An Athletic Trainer, Matthew Drescher, Lindsey Eberman
The Value Of Using Validated Competency-Based Assessments Across The Career Span Of An Athletic Trainer, Matthew Drescher, Lindsey Eberman
Clinical Practice in Athletic Training
No abstract provided.
The Development, Implementation, And Value Of The Athletic Training Milestones For Assessing Progressive Clinical Behaviors, Hollie Walusz, Eric Sauers, Mark Laursen, Forrest Pecha
The Development, Implementation, And Value Of The Athletic Training Milestones For Assessing Progressive Clinical Behaviors, Hollie Walusz, Eric Sauers, Mark Laursen, Forrest Pecha
Clinical Practice in Athletic Training
No abstract provided.
Perceptions And Use Of The Athletic Training Milestones In Education: A Report From The Aate Research Network, Cailee E. Welch Bacon, Barton E. Anderson, Julie M. Cavallario, Bonnie L. Van Lunen, Lindsey E. Eberman
Perceptions And Use Of The Athletic Training Milestones In Education: A Report From The Aate Research Network, Cailee E. Welch Bacon, Barton E. Anderson, Julie M. Cavallario, Bonnie L. Van Lunen, Lindsey E. Eberman
Clinical Practice in Athletic Training
The Athletic Training Milestones (AT Milestones) were developed as an evaluation tool to capture the breadth and depth of athletic training knowledge, skills, and behavior. However, it is unclear whether athletic training programs are implementing this tool or how they are implementing it to gauge the clinical progression of students or residents in athletic training. We aimed to explore the perceptions and use of the AT Milestones among educators in athletic training programs. We used a cross-sectional, web- based survey with open-ended questions to collect data from athletic training programs on if and how they implement the AT Milestones at …
Microplastic Accumulation In The Marsh Periwinkle (Littoraria Irrorata), Sarah Keaton, Hannah Lukhard, Eli Windy, Manar Hasan
Microplastic Accumulation In The Marsh Periwinkle (Littoraria Irrorata), Sarah Keaton, Hannah Lukhard, Eli Windy, Manar Hasan
DePaul Discoveries
Contamination of oceans by microplastics (mm) currently poses a major threat to aquatic and terrestrial ecosystems worldwide. Recent attention towards this issue has raised questions about the extent to which microplastics have accumulated in the environment, and has led to an increase in studies on the effects of microplastics in various organisms. However, levels of contamination in protected natural areas are still largely unexplored, yet can offer an important empirical perspective on the issue. In addition, little is known about the potential effects of microplastics on behavior in the field. This research was conducted within the protected ACE Basin National …
Lmpact Of Salmonella Enterica Effector Proteins On Plant Physiology And Immunity, Sara Qader
Lmpact Of Salmonella Enterica Effector Proteins On Plant Physiology And Immunity, Sara Qader
Honors Theses
Salmonella enterica serovar Typhimurium are primarily recognized as pathogens associated with foodborne illnesses in humans. Salmonella also can colonize plant tissue effectively. The pathogen relies on type III secretion systems (T3SS) to infect mammals and plants successfully and counteract the immune response. Understanding the molecular mechanisms underlying Salmonella’s interactions with plants is essential for developing effective strategies to reduce its impact on human health and agriculture. Effector proteins, known for their role in modulating host-pathogen interactions, have been implicated in the virulence and pathogenicity of Salmonella. However, their specific functions and contributions to Salmonella’s interactions with plants remain largely unknown. …
Phenotypic Plasticity In Pigmentation: Investigating Coloration Patterns In Anablepsoides Hartii Population Responses To Environmental Variation, Jasdeep Kaur
2024 Spring Honors Capstone Projects - Archive
In this study, we investigated the color-changing capabilities of Trinidadian killifish, Anablepsoides hartii, through exposure to varied color conditions over defined periods of time. Our experiment aims to understand adaptive responses when killifish are subjected to different color backgrounds, exploring their mechanisms to change color. By subjecting the species to different environmental color variations, we sought to discern color change patterns, which could indicate adaptive responses related to camouflage, stress, behavior clues, and physiological adjustments. Carefully controlling environmental factors, we monitored and documented the fishes’ color changes, aiming to provide insights into the interplay between fish’s developmental plasticity and its …
Thamnophis And Their Toxic Relationship: Testing For Intraspecific Venom Variation In The Common Garter Snake, Thamnophis Sirtalis, Grace E. Coppinger
Thamnophis And Their Toxic Relationship: Testing For Intraspecific Venom Variation In The Common Garter Snake, Thamnophis Sirtalis, Grace E. Coppinger
Poster Presentations
Project Objective - Estimate intraspecific venom variation between male and female garter snakes collected from the University of South Alabama of different sizes.
Effects Of Sex, Cadmium, And High-Fat-Diet On Accumulation Of Essential Metals In Mouse Kidneys., Justin Y. Xu, Adam Gaweda, Michelle Barati, Michael Merchant, Jun Cai, Lu Cai, Madhavi Rane
Effects Of Sex, Cadmium, And High-Fat-Diet On Accumulation Of Essential Metals In Mouse Kidneys., Justin Y. Xu, Adam Gaweda, Michelle Barati, Michael Merchant, Jun Cai, Lu Cai, Madhavi Rane
Undergraduate Research Events
Effects of cadmium and obesity on accumulation of essential metals in mouse kidney were evaluated. Starting in utero and continuing until 24 weeks postweaning, male/female mice were exposed to cadmium (0, 0.5, or 5 ppm) and were fed on Normal Diet (ND)/High-Fat Diet (HFD) post weaning. ICP-MS analysis was performed to evaluate cadmium and essential metals absorbed in the kidneys of aforementioned mice. Metals data obtained was analyzed using MANOVA with multiple comparisons (Bonferroni correction with p
A Dietary Variety: Analyzing The Impacts Of Diverse Vs Worm-Exclusive Diets On The Blanding’S Turtle (Emydoidea Blandingii), Caitlin F. Copenhaver
A Dietary Variety: Analyzing The Impacts Of Diverse Vs Worm-Exclusive Diets On The Blanding’S Turtle (Emydoidea Blandingii), Caitlin F. Copenhaver
Honors Program: Senior Projects (Public)
The endangered Blanding’s turtle (Emydoidea blandingii) is a species with a generally diverse diet in their freshwater wetland habitats; they consume prey such as crayfish and snails. In captivity, these options often become more limited due to logistical reasons. As ex situ conservation efforts such as captive rearing and head-starting are becoming more popular for this species, it will be important to gain more insight on how these more limited diets might affect these turtles. This study describes a comparison between diets in six captive Blanding’s turtles from September to November of 2023, with three turtles receiving only …
Investigating Optimal Laboratory Growth Conditions Of Gracilibacillus Halotolerans In Media Supplemented With Salt, Isaac Young
Investigating Optimal Laboratory Growth Conditions Of Gracilibacillus Halotolerans In Media Supplemented With Salt, Isaac Young
Annual Research Symposium
As interest continues to grow in the field of persister cells and their morphology, there arises an ever-evolving desire to further understand specific strains of bacteria that exemplify the qualities of seemingly anomalous survival regardless of anti-bacterial treatment. In the case of the Gracilibacillus halotolerans, a halotolerant extremophile extracted from the Great Salt Lake with known persistent characteristics, uncovering its optimal growth conditions was essential for future investigations. Identifying the optimal salinity for the growth of G. halotolerans will allow us to standardize our growth methods, uncover several mechanisms of saline tolerance, and add to future investigations of persistence with …
Estimation Of Probability Of Habitat Use Of Roosevelt Elk On The Olympic Peninsula, Vincent Michael Gugliotti
Estimation Of Probability Of Habitat Use Of Roosevelt Elk On The Olympic Peninsula, Vincent Michael Gugliotti
Undergraduate Theses, Professional Papers, and Capstone Artifacts
Estimating the probability of habitat use for a particular species is crucial to the direct management and conservation of that species. Without knowledge of habitat preferences, managers cannot effectively focus efforts on vital resources or landscape types. However, modelling probability of habitat use can be done in several ways which leaves room for variation and uncertainty in the estimates produced by each method. This study is an examination of the variation between two estimates of probability of habitat use while focusing on a particular subspecies of elk that inhabits a unique ecosystem relative to other elk subspecies. I modeled elk …
Compliance And Roughness In Relation To Gecko Locomotion, Brooklynn Campbell
Compliance And Roughness In Relation To Gecko Locomotion, Brooklynn Campbell
Williams Honors College, Honors Research Projects
Geckos move efficiently through a variety of substrates, from swaying branches to wooden fences to the sides of structures. Previous studies have studied locomotion and adhesion on manmade and rough substrates; however, the work is limited when discussing locomotion and adhesion as a relationship of compliance and roughness. Ease of movement was quantified by speed, under the assumption that the faster a gecko moves, the easier it is for the gecko to cross the substrate. Gehyra oceanica geckos from Moorea, French Polynesia were tested on substrates varying in compliance and roughness with four different treatments in total. Compliance, roughness, and …
Eliciting The Effect Free Roaming Cats Have On Native Hawaiian Wildlife Using Stable Isotope Analysis, Erin Salano
Eliciting The Effect Free Roaming Cats Have On Native Hawaiian Wildlife Using Stable Isotope Analysis, Erin Salano
Theses and Dissertations--Biology
This study measured the concentration of the stable isotopes Carbon and Nitrogen within the tissues of free-roaming and indoor cats on the Hawai’ian islands. This study aimed to determine whether cats are consuming more human provisioned or wild food sources and whether their diet is having a negative impact on endemic species; with a focus on assessing impacts on conserved bird populations. We hypothesized that human provisioned food sources have higher levels of carbon due to the amount of corn fillers within them, while wild food sources are more Nitrogen rich. We found that indoor and feral cats sampled from …
Understanding Cellular Dynamics: Investigating A Yeast-Two Hybrid Protein-Protein Interaction, Riok3:Cse1l; Also By Investigating Trogocytosis Of Cd4+ T Cells From Rhog-/- Mice, Brittnee N. Crane
Graduate Student Theses, Dissertations, & Professional Papers
Rift Valley fever virus (RVFV) is a negative-sense virus that was first described in 1930 when it was isolated from sheep during an outbreak. In the last 25 years there has been a spread in the geographical range into the Middle East. The most susceptible animals are cattle, goats, sheep, and camels. The most overt symptom of livestock infection is that infected pregnant animals experience spontaneous abortion. Humans are susceptible as well and symptoms range from mild febrile illness to liver necrosis, blindness, hemorrhagic fever, and death. An important player in the cellular innate response to RVFV infections is the …
Tim/Tam Receptors: A Potential Biomarker For Predicting Sensitivity To Zika Virus-Induced Oncolysis In Non-Small Cell Lung Cancers, Shankari Somasekar
Tim/Tam Receptors: A Potential Biomarker For Predicting Sensitivity To Zika Virus-Induced Oncolysis In Non-Small Cell Lung Cancers, Shankari Somasekar
Honors Undergraduate Theses
Non-small cell lung cancers (NSCLC) constitute 80-85% of lung cancers and are the leading cause of cancer-related deaths globally. The most common cause is prolonged smoking. Current treatment options for NSCLC include surgery, radiation, chemotherapy, targeted drug therapy, and immunotherapy. Although these medications are effective in the short term, patients often face issues of drug resistance and debilitating side effects with prolonged use. Currently, the use of Zika virus (ZIKV) is being researched as a possible alternative treatment for cancer, which minimizes side effects and the risk of drug resistance. TIM/TAM proteins are identified as the putative ZIKV receptors on …