Open Access. Powered by Scholars. Published by Universities.®

Plant Pathology Commons™

Open Access. Powered by Scholars. Published by Universities.®

Department of Plant Pathology: Faculty Publications

Discipline
Keyword
Publication Year

Articles 601 - 630 of 660

Full-Text Articles in Plant Pathology

Conjugative Plasmid In Corynebacterium Flaccumfaciens Subsp. Oortii That Confers Resistance To Arsenite, Arsenate, And Antimony(Iii), Carol A. Hendrick, William P. Haskins, Anne K. Vidaver Jul 1984

Conjugative Plasmid In Corynebacterium Flaccumfaciens Subsp. Oortii That Confers Resistance To Arsenite, Arsenate, And Antimony(Iii), Carol A. Hendrick, William P. Haskins, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

Gene transfer systems for phytopathogenic corynebacteria have not been reported previously. In this paper we describe a conjugative 46-megadalton plasmid (pDG101) found in Corynebacterium flaccumfaciens subsp. oortii COIOI that mediates resistance to arsenite, arsenate, and antimony(III). Transfer of the plasmid from CO101 to four other strains from the C. flaccumfaciens group occurred between cells immobilized on nitrocellulose filters or on agar surfaces. Transconjugant strains expressed the same levels of metal resistance as the donor strain and were able to act as donor strains in subsequent matings. The physical presence of the plasmid was detected by agarose gel electrophoresis. Arsenite-sensitive derivatives …

Go to article

Clavibacter: A New Genus Containing Some Phytopathogenic Coryneform Bacteria, Including Clavibacter Xyli Subsp. Xyli Sp. Nov., Subsp. Nov. And Clavibacter Xyli Subsp. Cynodontis Subsp. Nov., Pathogens That Cause Ratoon Stunting Disease Of Sugarcane And Bermudagrass Stunting Disease, Michael J. Davis, A. Graves Gillaspie Jr, Anne K. Vidaver, Russell W. Harris Apr 1984

Clavibacter: A New Genus Containing Some Phytopathogenic Coryneform Bacteria, Including Clavibacter Xyli Subsp. Xyli Sp. Nov., Subsp. Nov. And Clavibacter Xyli Subsp. Cynodontis Subsp. Nov., Pathogens That Cause Ratoon Stunting Disease Of Sugarcane And Bermudagrass Stunting Disease, Michael J. Davis, A. Graves Gillaspie Jr, Anne K. Vidaver, Russell W. Harris

Department of Plant Pathology: Faculty Publications

A total of 23 strains of coryneform bacteria that cause ratoon stunting disease of sugarcane and Bermudagrass stunting disease were examined. These included 17 sugarcane strains from Florida, Louisiana, South Africa, Brazil, and Japan and six Bermudagrass strains from Florida and Taiwan. The sugarcane and Bermudagrass strains contained 2,4-diaminobutyric acid, rhamnose, and fucose in their cell walls, suggesting a relationship to some other phytopathogenic coryneform bacteria and related organisms with similar cell wall compositions. The cellular protein patterns of the sugarcane and Bermudagrass strains after polyacrylamide gel electrophoresis resembled the patterns of Corynebacterium michiganense subspecies more than the patterns of …

Go to article

Book Review: Plant Infectious Agents, Myron K. Brakke Jan 1984

Book Review: Plant Infectious Agents, Myron K. Brakke

Department of Plant Pathology: Faculty Publications

This is not the book to go to for exhaustive coverage or detailed experimental protocol, but it is excellent for a concise version of recent developments in the molecular biology of plant viruses.

Go to article

Two Purified Rnas Of Soil-Borne Wheat Mosaic Virus Are Needed For Infection, Yukio Shirako, Myron K. Brakke Jan 1984

Two Purified Rnas Of Soil-Borne Wheat Mosaic Virus Are Needed For Infection, Yukio Shirako, Myron K. Brakke

Department of Plant Pathology: Faculty Publications

RNAs of soil-borne wheat mosaic virus (SBWMV) from virions 281 nm, 138 nm and 92 nm long (designated here by relative lengths as 1∙0L, 0∙SL and 0∙35L, respectively), were isolated and purified by three cycles of sucrose density gradient centrifugation. Infectivity assays with these RNAs proved the bipartite nature of SBWMV, the combination of 1∙0L and either 0∙5L or 0∙35L RNAs being required for infection and for multiplication of progeny viruses. The 0∙5L RNA underwent deletion mutation, producing smaller variants with various sizes, of which 0∙4L and 0∙35L RNAs were confirmed to be functional in combination with 1∙0L RNA. The …

Go to article

Mutations, The Abberant Ratio Phenomenon, And Virus Infection Of Maize, Myron K. Brakke Jan 1984

Mutations, The Abberant Ratio Phenomenon, And Virus Infection Of Maize, Myron K. Brakke

Department of Plant Pathology: Faculty Publications

Viruses interact genetically in various ways with bacterial and animal hosts, e.g. by lysogeny in bacteriophage and by incorporating a DNA copy of retrovirus RNA into the host genome. These phenomena lead to mutations, to the transfer of host genes by viruses, and to latent viruses being transmitted from generation to generation essentially as host genes. In contrast, there are no known examples of the incorporation of viral genomes into plant DNA.

Go to article

Spontaneous Deletion Mutation Of Soil-Borne Wheat Mosaic Virus Rna Ii, Yukio Shirako, Myron K. Brakke Jan 1984

Spontaneous Deletion Mutation Of Soil-Borne Wheat Mosaic Virus Rna Ii, Yukio Shirako, Myron K. Brakke

Department of Plant Pathology: Faculty Publications

The wild-type (WT) isolate of soil-borne wheat mosaic virus has two species of rodshaped virions 281 nm and 138 nm in length, which are designated as virions 1∙0L and 0∙5L, respectively. We reported previously that virions shorter than 0∙5L arose in assay plants inoculated with separated and recombined 1∙0L RNA and 0∙5L RNA and suggested that these short virions were caused by deletion mutation of 0∙5L RNA. We now report that these short virions arose after a period of several months in wheat plants that had been inoculated manually with unpurified WT isolate, and also when plants infected naturally in …

Go to article

Biosynthesis And Degradation Of Storage Protein In Spores Of The Fungus Botryodiplodia Theobromae, Gary Petersen, Kurt Dahlberg, James L. Van Etten Aug 1983

Biosynthesis And Degradation Of Storage Protein In Spores Of The Fungus Botryodiplodia Theobromae, Gary Petersen, Kurt Dahlberg, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Muiridin, a spore-specific protein of the fungus Botryodiplodia theobromae, comprises about 25% of the mature pycnidiospore protein. It has an apparent molecular weight of 16,000 to 17,000 and is rich in glutamine, asparagine, and arginink. Muiridin is synthesized in developing spores via a precursor with an apparent molecular welght of 24,000. Two other polypeptides present in young developing spores with apparent molecular weights of 18,000 and 15,000 are immunologically related to muiridin. We propose a pathway for muiridin synthesis. Muiridin is actively degraded during the germination of spores from 30-day old cultures. This degradation is independent of exogenous amino …

Go to article

Effects Of Two Species Of Va Mycorrhizal Fungi On Drought Tolerance Of Winter Wheat, Michael F. Allen, Michael G. Boosalis Jan 1983

Effects Of Two Species Of Va Mycorrhizal Fungi On Drought Tolerance Of Winter Wheat, Michael F. Allen, Michael G. Boosalis

Department of Plant Pathology: Faculty Publications

Roots and soils from western Nebraska fields of native and planted grasslands, and winter wheat of varied fallow-wheat cultivation duration, were evaluated for vesicular-arbuscular (VA) mycorrhizal root infection and spore numbers and types. Increased cultivation decreased percentage mycorrhizal infection in wheat and reduced spore numbers of Glomus fasciculatus, the dominant VA mycorrhizal fungus in these soils. Spore numbers of other VA mycorrhizal fungi did not change significantly with cultivation although mean numbers of G. mosseae increased with continued wheat production. Water relations and growth were determined for greenhouse-grown non-mycorrhizal, G. fasciculatus-infected, and G. mosseae-infected wheat in wet …

Go to article

Taxonomic Limits Of The Genus Nagelus (Thorne And Malek, ]968) Siddiqi, 1979 With A Description Of Nagelus Borealis N.Sp. From Alaska, Thomas O. Powers, James G. Baldwin, A. H. Bell Jan 1983

Taxonomic Limits Of The Genus Nagelus (Thorne And Malek, ]968) Siddiqi, 1979 With A Description Of Nagelus Borealis N.Sp. From Alaska, Thomas O. Powers, James G. Baldwin, A. H. Bell

Department of Plant Pathology: Faculty Publications

Tire genus Nagelus (Thorne and Malek, 1968) Siddiqi, 1979 is modified and a new species from Alaska is described. The combination of scanning electron microscopy and light microscopy permits the characterization of Nagelus spp. as having a broadly oval face pattern, no longitudinal striations on the lip region, deirids surrounded by six incisures, and an irregularly tapering tail with a large hyaline region. Nagelus leptus (Allen, 1955) Siddiqi, 1979, N. alpensis Doucet and Luc, 1981, N. camelliae (Kheiri, 1972) Siddiqi, 1979, N. jamelensis (Nesterov, 1973) Siddiqi, 1979, and N. obscurus (Allen, 1955) n. comb. are retained on this basis. Nagelus …

Go to article

Viruses Of Symbiotic Chlorella-Like Algae Isolated From Paramecium Bursaria And Hydra Viridis, James L. Van Etten, Russel H. Meints, Daniel Kuczmarski, Dwight E. Burbank, Kit Lee Jan 1982

Viruses Of Symbiotic Chlorella-Like Algae Isolated From Paramecium Bursaria And Hydra Viridis, James L. Van Etten, Russel H. Meints, Daniel Kuczmarski, Dwight E. Burbank, Kit Lee

Department of Plant Pathology: Faculty Publications

We previously reported that isolation of symbiotic Chloreila-like algae from the Florida strain of Hydra viridis induced replication of a virus (designated HVCV-1) in the algae. We now report that isolation ofsymbiotic Chlorella-like algae from four other sources of green hydra and one source ofthe protozoan Paramecium bursaria also induced virus synthesis. Algae from one of these hydra contained a virus identical to HVCV-1 (based on its rate of sedimentation, buoyant density, reaction to HVCV-1 antiserum, and DNA restriction fragments) whereas algae from the other three hydra contained. another similar, but distinct, virus (designated HVCV-2). The virus from the paramecium …

Go to article

Influence Of Vesicular-Arbuscular Mycorrhizae On Water Movement Through Bouteloua Gracilis (H.B.K.) Lag Ex Steud, Michael F. Allen Jan 1982

Influence Of Vesicular-Arbuscular Mycorrhizae On Water Movement Through Bouteloua Gracilis (H.B.K.) Lag Ex Steud, Michael F. Allen

Department of Plant Pathology: Faculty Publications

Root growth characteristics and water transport were compared between non-mycorrhizal Bouteloua gracilis and vesicular-arbuscular mycorrhizal Bouteloua gracilis infected with Glomus fasciculatus. Mycorrhizal plants did not have significantly different leaf area or root length from non-mycorrhizal plants, hut did have significantly fewer and shorter root hairs. Mycorrhizal plants had 50% lower leaf resistance with no change in leaf or root water potentials; thus transpiration was increased 100% with a 50% reduction in whole-plant, soil-to-root and root-to-leaf resistance. Assuming that the difference in water uptake was due to transport via the mycorrhizal hyphae, estimated fungus-to-root transport was 2◦8 x 10-5 …

Go to article

The Plant Pathogenic Corynebacteria, Anne K. Vidaver Jan 1982

The Plant Pathogenic Corynebacteria, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

The plant pathogenic corynebacteria are a fascinating group of bacteria that produce effects as diverse as any in the microbial world, ranging from wilts to abnormal growths. Several new pathogens have been discovered since this group was reviewed by Jensen (75), Starr (157), and Lelliott (96). Most subsequent reviews have dealt with taxonomy. The areas covered in this review necessarily reflect my biases and limitations. However, this review attempts to be comprehensive in some areas, and it calls attention to other areas that would benefit from more detailed investigation.

Go to article

Influence Of Phosphate Source On Vesicular-Arbuscular Mycorrhizae Of Bouteloua Gracilis, Michael F. Allen, James C. Sexton, Thomas S. Moore Jr., Martha Christensen Jan 1981

Influence Of Phosphate Source On Vesicular-Arbuscular Mycorrhizae Of Bouteloua Gracilis, Michael F. Allen, James C. Sexton, Thomas S. Moore Jr., Martha Christensen

Department of Plant Pathology: Faculty Publications

Non-mycorrhizal and vesicular-arbuscular mycorrhizal Bouteloua gracilis infected with Glomus fasciculatus were grown in defined media containing different phosphate sources and compared with respect to phosphate content and form, biomass, chlorophyll concentration, and root phosphatase activity. The phosphate sources were sodium monobasic phosphate, a mixture of sodium monobasic phosphate and calcium phytate, and calcium phytate. Inositol and inositol plus calcium were added to the sodium phosphate medium as additional treatments. Mycorrhizal infection was highest in roots of plants grown in the presence of phytate (75%). Lower root infection levels were noted in plants from the sodium phosphate (19%) and mixed phosphate …

Go to article

Recessive Alleles Found At R And C Loci In Maze Stalks Showing Aberrant Ratio At The A Locus, Myron K. Brakke, R. G. Samson, W. A. Compton Jan 1981

Recessive Alleles Found At R And C Loci In Maze Stalks Showing Aberrant Ratio At The A Locus, Myron K. Brakke, R. G. Samson, W. A. Compton

Department of Plant Pathology: Faculty Publications

Corn stocks showing virus-induced aberrant ratio (AR) at the “A” locus were found to have recessive alleles at the R and/or C loci. Since by the known pedigree these loci should be homozygous dominant, the results suggest an inactivation of maize genes by a mechanism as yet unknown. The presence of recessive alleles at these additional loci can explain the segregation ratios obtained in these particular stocks.

Go to article

Effects Of Reduced Tillage And Multiple Cropping On Plant Diseases, Donald R. Sumner, Ben Doupnik Jr., M. G. Boosalis Jan 1981

Effects Of Reduced Tillage And Multiple Cropping On Plant Diseases, Donald R. Sumner, Ben Doupnik Jr., M. G. Boosalis

Department of Plant Pathology: Faculty Publications

In the past, tillage research on plant diseases was concerned primarily with practices that buried plant residues in single-crop production systems. The burial of plant debris to destroy pathogens is an ancient agricultural prac­ tice (34). Each crop was tested as a single entity, and most crop rotations were based on one crop per year. Interest in notill and conservation tillage systems and mUltiple cropping has increased in the past two decades be­ cause of the scarcity and increased cost of fossil fuels, periodic world food shortages, and the concern over soil erosion (73,110). These concerns are of such paramount …

Go to article

Comparative Water Relations And Photosynthesis Of Mycorrhizal And Non-Mycorrhizal Bouteloua Gracilis H.B.K. Lag Ex Steud., Michael F. Allen, William K. Smith, Thomas S. Moore Jr., Martha Christensen Jan 1981

Comparative Water Relations And Photosynthesis Of Mycorrhizal And Non-Mycorrhizal Bouteloua Gracilis H.B.K. Lag Ex Steud., Michael F. Allen, William K. Smith, Thomas S. Moore Jr., Martha Christensen

Department of Plant Pathology: Faculty Publications

The rangeland grass, Bouteloua gracilis was inoculated with its mycorrhizal symbiont, Glomus fasciculatus, to determine the influence of vesicular-arhuscular mycorrhizae on water status, stomatal behaviour and photosynthesis as well as gross plant morphology, biomass and phosphorus content. Mycorrhizal infection increased transpiration rates by over 100% with 50 to 70% lower leaf resistances to water vapour diffusion. Leaf xylem pressure was not different between mycorrhizal and non-mycorrhizal plants indicating that whole-plant resistance to water transport was reduced by more than 50%. Photosynthetic rates under saturating light conditions increased 68% with infection as a consequence of a 33% reduction in stomatal …

Go to article

In Vitro Translation Of The Three Bacteriophage Φ6 Rnas, Diane A. Cuppels, James L. Van Etten, Dwight E. Burbank, Leslie C. Lane, Anne K. Vidaver Jul 1980

In Vitro Translation Of The Three Bacteriophage Φ6 Rnas, Diane A. Cuppels, James L. Van Etten, Dwight E. Burbank, Leslie C. Lane, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

In vitro translation of the three single-stranded RNAs transcribed in vitro by bacteriophage φ6 RNA polymerase revealed that the large RNA codes for phage proteins P1, P2, P4, and P7, the medium RNA codes for P3, P6, and P10, and the small RNA codes for P5, P8, and P9.

Go to article

Semiconservative Synthesis Of Single-Stranded Rna By Bacteriophage Φ6 Rna Polymerase, James L. Van Etten, Dwight E. Burbank, Diane A. Cuppels, Leslie C. Lane, Anne K. Vidaver Feb 1980

Semiconservative Synthesis Of Single-Stranded Rna By Bacteriophage Φ6 Rna Polymerase, James L. Van Etten, Dwight E. Burbank, Diane A. Cuppels, Leslie C. Lane, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

The RNA polymerase in the nucleocapsid of seudomonas phaseolicola bacteriophage φ6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a semiconservative (displacement) mechanism. Approximately 23%, 63%, and 65% of the nucleocapsid particles in the assay mixture synthesized at least one round of large, medium, and small single-stranded RNA molecules, respectively. Some of these particles reinitiated synthesis such that an average of 1.5 large, 33 medium, and 24 small singlestranded RNAs were synthesized from each double-stranded RNA.

Go to article

Glutaraldehyde Nonfixation Of Isolated Viral And Yeast Rnas, Willem G. Langenberg Jan 1980

Glutaraldehyde Nonfixation Of Isolated Viral And Yeast Rnas, Willem G. Langenberg

Department of Plant Pathology: Faculty Publications

The RNAs of brome mosaic (BMV), barley stripe mosaic (BSMV), and tobacco mosaic (TMV) viruses were inactivated by reaction with buffered glutaraldehyde. Glutaraldehyde did not fix 4% BMV-RNA, 20% t-RNA, 5% polyadenylic acid, or 5% adenosine monophosphate into water-insoluble precipitates, or gels, in distilled water or in low or high ionic strength buffers nor did it change their ultraviolet (UV) spectra. Two SDS- and phenolpurified commercial yeast RNA preparations from different sources gave UV spectra typical of pure RNA, but could not be freed of a contaminant that reacted with glutaraldehyde by forming a precipitate. The yeast RNAs did not …

Go to article

Presence Of A Major (Storage?) Protein In Dormant Spores Of The Fungus Botryodiplodia Theobromae, James L. Van Etten, S. N. Freer, B. K. Mccune May 1979

Presence Of A Major (Storage?) Protein In Dormant Spores Of The Fungus Botryodiplodia Theobromae, James L. Van Etten, S. N. Freer, B. K. Mccune

Department of Plant Pathology: Faculty Publications

Approximately 23% of the protein isolated from dormant spores of Botryodiplodia theobromae consisted of a single polypeptide; the polypeptide is probably degraded during germination.

Go to article

Bacteriocin-Like Substances Produced By Rhizobium Japonicum And Other Slow-Growing Rhizobia, D. C. Gross, A. K. Vidaver Dec 1978

Bacteriocin-Like Substances Produced By Rhizobium Japonicum And Other Slow-Growing Rhizobia, D. C. Gross, A. K. Vidaver

Department of Plant Pathology: Faculty Publications

Bacteriocin-like substances were commonly produced by slow-growing Rhizobium japonicum and cowpea rhizobia on an L-arabinose medium. Antagonism between strains of R. japonicum was not detected in vitro; however, such strains were often sensitive to some bacteriocins produced by cowpea rhizobia. Inhibitory zones (2 to 8 mm from colony margins), produced by 58 of 66 R. japonicum test strains, were reproducibly detected with Corynebacterium nebraskense as an indicator. Quantitative production was not related to symbiotic properties of effective strains, since nine noninfective strains and one ineffective strain produced bacteriocin. Eight R. japonicum strains that did not produce bacteriocin nevertheless formed effective …

Go to article

Simple Procedure For Disruption Of Fungal Spores, James L. Van Etten, Shelby Freer Mar 1978

Simple Procedure For Disruption Of Fungal Spores, James L. Van Etten, Shelby Freer

Department of Plant Pathology: Faculty Publications

A simple, inexpensive method for disrupting dormant fungal spores is described.

Go to article

Purification Of Some Legume Carlaviruses, Venkateswarlu Veerisetty, Myron K. Brakke Jan 1978

Purification Of Some Legume Carlaviruses, Venkateswarlu Veerisetty, Myron K. Brakke

Department of Plant Pathology: Faculty Publications

Purification schemes were developed which yielded 0.7 to 1.0 mg of alfalfa latent virus (ALV) and pea streak virus (PSV) and 0.1 to 0.3 mg of red clover vein mosaic virus (RCVMV) per gram pea cullivar Lincoln plant tissue (excluding roots). The freezing of the tissue and the use of an appropriate extraction buffer were crucial. Virus from sap was precipitated by 6%(w/v) polyethylene glycol (PEG, MW 6,000) and concentrated by two cycles of differential centrifugation. Partially purified virus preparations had a single nucleoprotein component in rate-zonal sucrose and equilibrium cesium chloride density gradient centrifugation. The virus preparations did not …

Go to article

Purification Of Some Legume Carlaviruses, Venkateswarlu Veerisetty, Myron K. Brakke Jan 1978

Purification Of Some Legume Carlaviruses, Venkateswarlu Veerisetty, Myron K. Brakke

Department of Plant Pathology: Faculty Publications

Purification schemes were developed which yielded 0.7 to 1.0 mg of alfalfa latent virus (ALV) and pea streak virus (PSV) and 0.1 to 0.3 mg of red clover vein mosaic virus (RCVMV) per gram pea cullivar Lincoln ) plant tissue (excluding roots). The freezing of the tissue and the use of an appropriate extraction buffer were crucial. Virus from sap was precipitated by 6%(w/v) polyethylene glycol (PEG, MW 6,000) and concentrated by two cycles of differential centrifugation. Partially purified virus preparations had a single nucleoprotein component in rate-zonal sucrose and equilibrium cesium chloride density gradient centrifugation. The virus preparations did …

Go to article

Nitrogen Fixation (Acetylene Reduction) Associated With Roots Of Winter Wheat And Sorghum In Nebraska, Wayne L. Pedersen, K. Chakrabarty, R. V. Klucas, Anne K. Vidaver Jan 1978

Nitrogen Fixation (Acetylene Reduction) Associated With Roots Of Winter Wheat And Sorghum In Nebraska, Wayne L. Pedersen, K. Chakrabarty, R. V. Klucas, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

Root segments and root-soil cores (6.5-cm diameter) from fields and nurseries of winter wheat and sorghum were tested for N2 fixation by using the acetylene reduction assay. Wheat samples (~1,200) from 109 sites generally had low or no activity (0 to 3.1 nmol of C2H4 produced per h per g [dry weight] of root segments), even after 24 h of incubation. However, a commercial field of Scout 66, located in western Nebraska, exhibited appreciable activity (290 nmol of C2H4 produced per h per g [dry weight] of root segments). Of 400 sorghum lines …

Go to article

Alfalfa Latent Virus, A Naturally Occurring Carlavirus In Alfalfa, Yenkateswarlu Veerisetty, Myron K. Brakke Jan 1977

Alfalfa Latent Virus, A Naturally Occurring Carlavirus In Alfalfa, Yenkateswarlu Veerisetty, Myron K. Brakke

Department of Plant Pathology: Faculty Publications

Alfalfa latent virus (ALV), a new member of the carlavirus group, was isolated From alfalfa (Medicago sarivo) clones from rbe University of Nebraska experimental plots and from the Farmers' fields. The pea aphid, Acvrihosiphon pisum, transmitted this virus to M. sativa, Pisum sativum, and Viria faba. but not to Trifolium pratense. It was also sap transmissible to V. faba, V. villoso, and P. sarivum, but was not seed-borne in P. sariuum. In V. Jaba sap ALV was infectious at 65 but not at 70 C in 10 min. or …

Go to article

Sedimentation Coefficients Of The Virions Of Soil-Borne Wheat Mosaic Virus, Myron K. Brakke Jan 1977

Sedimentation Coefficients Of The Virions Of Soil-Borne Wheat Mosaic Virus, Myron K. Brakke

Department of Plant Pathology: Faculty Publications

The sedimentation coefficient of virion I of soil-borne wheat mosaic virus was estimated to be 219S, the same, within error, as the sedimentation coefficient of the dimer of virion II, estimated to be 218S. The monomer of virion II sedimented at 177S, and was 138 nm long. Another strain of the virus had a virion II (designated lIb) that was 92 nm long and sedimented at 159S. The two virus strains coexist in some fields.

Go to article

Comparative Properties Of Bacteriophage Φ6 And Φ6 Nucleocapsid, James L. Van Etten, Les Lane, Carlos Gonzalez, James Partridge, Anne Vidaver May 1976

Comparative Properties Of Bacteriophage Φ6 And Φ6 Nucleocapsid, James L. Van Etten, Les Lane, Carlos Gonzalez, James Partridge, Anne Vidaver

Department of Plant Pathology: Faculty Publications

Nonionic detergent treatments released a nucleocapsid from the enveloped bacteriophage φ6. The nucleocapsid sedimented at nearly the same rate as the whole phage in sucrose density gradients, but the buoyant density in Cs2SO4 changed from 1.22 g/cm3 for the whole phage to 1.33 g/cm3 for the nucleocapsid. The detergent completely removed the lipid and 5 of the 10 proteins from the phage. Surface labeling of the phage and nucleocapsid with 125I revealed that protein P3 was on the outer surface of the whole phage and P8 was on the surface of the nucleocapsid. Both …

Go to article

Prospects For Control Of Phytopathogenic Bacteria By Bacteriophages And Bacteriocins, Anne K. Vidaver Jan 1976

Prospects For Control Of Phytopathogenic Bacteria By Bacteriophages And Bacteriocins, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

The last review on bacteriophages and bacteriocins of phytopathogenic bacteria was that of Okabe & Goto (98). All but one (21) of the subsequent reviews (II, 12, 60, 94, 104) have dealt primarily with viruses and bacteriocins of bacteria other than phytopathogens. The most recent microbiological compendia that in­ clude bacteriophages and bacteriocins are of limited value for phytobacteriolo­ gists because the authors generally overlook or ignore such agents (29, 47, 77, 119). However, Adam's classic .book (4), the review by Bradley (II), and the bibliography of Raettig (102) are still of value to phytobacteriologists as are reviews dealing principally …

Go to article

Pollution Of Surface Irrigation Waters By Plant Pathogenic Organisms, James R. Steadman, C. R. Maier, H. F. Schwartz, E. D. Kerr Aug 1975

Pollution Of Surface Irrigation Waters By Plant Pathogenic Organisms, James R. Steadman, C. R. Maier, H. F. Schwartz, E. D. Kerr

Department of Plant Pathology: Faculty Publications

Systematic sampling of waterways and irrigation runoff from agricultural lands in the North Platte Project of Nebraska in July and August of 1972–1974 demonstrated that phytopathogenic organisms were disseminated. The organisms monitored included the bean common blight bacterium Xanthomonas phaseoli, the bean white mold fungus Whetzelinia sclerotiorum and various nematodes. Although many types of nematodes often were recovered from irrigation water, Heterodera sp. cysts which cause significant disease problems in the valley were found infrequently. Patterns of movement of the bacterial and fungal organisms were correlated with previous or current season infection of bean plants. The short-term survival of X. …

Go to article

First Prev Next Last