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Full-Text Articles in Plant Pathology
Oxalic Acid Leaf Disk Soak Assay Is A New Possibility In Screening For Blight Resistance In Castanea Species, Kaitlyn Harden
Oxalic Acid Leaf Disk Soak Assay Is A New Possibility In Screening For Blight Resistance In Castanea Species, Kaitlyn Harden
Honors Theses
Chestnut blight is a disease caused by the ascomycete fungus Cryphonectria parasitica in the Castanea species. The fungus uses oxalic acid (OA) to attack the tree’s cells. Castanea dentata, the American chestnut, was wiped out by chestnut blight in the early to mid-20th century, but several East Asian Castanea species appear highly resistant to the fungus. To breed resistant American type trees, screening methods are used to enable selection of interspecific hybrids. The alternative small stem assay (aSSA) is a method of screening container-grown hybrid seedlings during their first growing season by directly infecting them with C. parasitica. Some plant …
A Small Stem Assay Using Hypovirulent Cryphonectria Parasitica To Screen Castanea Dentata Backcross F2 Families May Set The Stage For Long-Term Survival, Trish Nguyen
Honors Theses
By combining the results of blight resistance breeding and the application of hypovirulence as a biocontrol, populations of resistant hybrid trees could be deployed together with a less pathogenic strain of Cryphonectria parasitica (Murr.) Barr. I used a small stem assay to screen seedlings in thirteen half-sibling backcross F2 families with an attenuated strain of C. parasitica containing the Cryphonectria parasitica hypovirus-1 (CHV-1) Euro7 virus. The experiment was set up as a randomized complete block design in 2-gallon containers. Measurements of canker length and morphology were gathered at 90 days post-inoculation. Although statistically significant differences were seen between canker lengths …
A Small Stem Assay For Chestnut Blight Resistance In Hybrid American Chestnut Trees, Evelyn Odle
A Small Stem Assay For Chestnut Blight Resistance In Hybrid American Chestnut Trees, Evelyn Odle
Honors Theses
Cryphonectria parasitica (Murr.) Barr is the causal agent for the chestnut blight disease, which was brought to North America in the late 19th century on nursery stock from Japan (Anagnostakis 1987). A pandemic of chestnut blight lasted for the next half century, nearly wiping out all American chestnut trees (Castanea dentata Borkh.). Restoration works to restoring the American chestnut to its native range in North America’s canopy has been going on for the past century. The American Chestnut Foundation has strived to breed a resistant American chestnut by backcross breeding with Chinese species (C. mollissima Blume) since the 1980s. The …
The Effect Of Phosphite On Mycorrhiza Formation In American Chestnut (Castanea Dentata), Matthew T. Perkins
The Effect Of Phosphite On Mycorrhiza Formation In American Chestnut (Castanea Dentata), Matthew T. Perkins
Honors Theses
One of the primary hindrances to Castanea dentata restoration in the Southeast is the root rot disease caused by the fungus-like microorganism Phytophthora cinnamomi. Root rot can be combated by the application of mono- and di-potassium salts of phosphorous acid, which are marketed as phosphite fungicides. Despite its value in preventing infection by P. cinnamomi it is also thought that phosphite may impede root colonization by beneficial, ectomycorrhizal fungi. I hypothesized that plants given a routine application of phosphite will display fewer mycorrhizas in the root tips than those plants that were not treated with potassium phosphite. Therefore I attempted …