Plant Pathology Commons^™

Chlorella Viruses, Takashi Yamada, Hideki Onimatsu, James L. Van Etten

James Van Etten Publications

Chlorella viruses or chloroviruses are large, icosahedral, plaque‐forming, double‐stranded‐DNA— containing viruses that replicate in certain strains of the unicellular green alga Chlorella. DNA sequence analysis of the 330‐kbp genome of Paramecium bursaria chlorella virus 1 (PBCV‐1), the prototype of this virus family (Phycodnaviridae), predict ∼366 protein‐encoding genes and 11 tRNA genes. The predicted gene products of ∼50% of these genes resemble proteins of known function, including many that are completely unexpected for a virus. In addition, the chlorella viruses have several features and encode many gene products that distinguish them from most viruses. These products include: (1) multiple …

The Structure Of A T=169d Algal Virus, Pbcv-1, At 15Å Resolution, X. Yan, V. Bowman, N. H. Olson, James Gurnon, James L. Van Etten, Michael G. Rossmann, T. S. Baker

James Van Etten Publications

Paramecium bursaria chlorella virus 1 (PBCV-1) (genus Chlorovirus, family Phycodnaviridae), infects certain unicellular, exsymbiotic, chlorella-like green algae [1]. The PBCV-1 virion (~1x109 Da) has a linear dsDNA genome (330kbp) and contains at least 100 different proteins [1, unpublished data]. A published cryo-electron microscopy and three-dimensional (3D) image reconstruction of PBCV-1 (26Å resolution) revealed that the outer icosahedral glycoprotein capsid, with a maximum diameter of 1,900Å along the five-fold axis, consists of 1680 trimeric capsomers (trimer) and 12 pentameric capsomers (pentamer) [2]. These capsomers are arranged on a T=169d (h=7, k=8) quasi-equivalent lattice. The trimers and pentamers are organized into 20 …

Functional Implication Of The Trna Genes Encoded In The Chlorella Virus Pbcv-L Genome, Da Young Lee, Michael V. Graves, James L. Van Etten, Tae-Jin Choi

James Van Etten Publications

The prototype Chlorella virus PBCV-l encodes 11 tRNA genes and over 350 protein-encoding genes in its 330 kbp genome. Initial attempts to overexpress the recombinant A189/192R protein, a putative virus attachment protein, in E. coli strain BL21(DE3) SI were unsuccessful, and multiple protein bands were detected on Western blots. However, the full-length A189/192R recombinant protein or fragments derived from it were detected when they were expressed in E. coli BL21 CodonPlus (DE3) RIL, which contains extra tRNAs. Codon usage analysis of the a1891192r gene showed highly biased usage of the AGA and AUA codons compared to genes encoded by E. …

Glycosyltransferases Encoded By Viruses, Nicolas Markine-Goriaynoff, Laurent Gillet, James L. Van Etten, Haralambos Korres, Naresh Verma, Alain Vanderplasschen

James Van Etten Publications

Studies of cellular biology in recent decades have highlighted the crucial roles of glycans in numerous important biological processes, raising the concept of glycomics that is now considered as important as genomics, transcriptomics and proteomics. For millions of years, viruses have been co-evolving with their hosts. Consequently, during this co-evolution process, viruses have acquired mechanisms to mimic, hijack or sabotage host processes that favour their replication, including mechanisms to modify the glycome. The importance of the glycome in the regulation of host–virus interactions has recently led to a new concept called ‘glycovirology’. One fascinating aspect of glycovirology is the study …

Characterization Of A PorosTm-Fumonisin B1 Affinity Column For Isolating Ceramide Synthase From Rat Liver, S. Jernigan, W. B. Melchior Jr., G. R. Jenkins, K. L. Rowland, D. W. Roberts, P. C. Howard, H. Tolleson

Journal of the Arkansas Academy of Science

Fumonisin B1 is a mycotoxin produced by fungi of the genus Fusarium, common pathogens of corn and other grain plants. Toxic effects associated with fumonisin B1 include equine leukoencephalomacia, porcine pulmonary edema, rat renal carcinoma, and murine hepatocellular carcinoma. Increased risk for esophageal cancer in humans has been epidemiologically associated with consumption of corn contaminated with Fusarium, suggesting that fumonisin B1 may be involved. The biological effects of fumonisin B1 exposure result primarily from disruption of de novo sphingolipid biosynthesis via inhibition of ceramide synthase. Exposure of animals or cultured cells to fumonisin B1 results in the characteristic accumulation of …

Bulletin 4406a - Fungal Diseases Of Canola In Western Australia, Martin Barbetti, Ravjit Khangura

Bulletins 4000 -

GRDC - Grains Research & Development Corporation.

There has been dramatic growth in the canola industry in Western Australia, from 35,000 hectares in 1993 to 920,000 hectares in 1999. Along with this expansion in the area sown the incidence and severity of various fungal diseases have also increased, especially blackleg. This Bulletin describes the most important and most prevalent fungal diseases of canola in Western Australia.

Can Large Dsdna-Containing Viruses Provide Information About The Minimal Genome Size Required To Support Life?, James L. Van Etten

James Van Etten Publications

The genomes of a few viruses, such as Bacillus megaterium phage G (670 kb) and the chlorella viruses (330 to 380 kb), are larger than the predicted minimal genome size required to support life (ca. 320 kb). A comparison of the 256 proteins predicted to be required for life with the putative 376 proteins encoded by chlorella virus PBCV-1, as well as those encoded by other large viruses, indicates that viruses lack many of these “essential” genes. Consequently, it is unlikely that viruses will aid in determining the minimal number and types of genes required for life. However, viruses may …

Partial Characterization Of Bacteriophages Of Pseudomonas Syringae Pv.Tomato, Scott M. Minor, Russell O. Nordeen, Chad E. Paschall

Journal of the Arkansas Academy of Science

No abstract provided.

Bulletin 4294 - Virus Diseases Of Lupins, Roger Jones

Bulletins 4000 -

Two virus diseases pose a serious threat to lupins. These are caused by cucumber mosaic virus (CMV), which induces diseases in narrow-leafed and yellow lupins, and bean yellow mosaic virus (BYMV), which infects all types of cultivated lupins. The effects of the viruses on lupin plants range from subtle colour changes and leaf distortion to leaf shedding, stunting and death. Seed production is decreased or prevented. There is no cure, but lupin crops can be managed to minimise both virus spread and its persistence from year to year.

CMV and BYMV occur within all tissues in the plant, but do …

Role Of Endophytes In Tall Fescue, E. L. Piper, C. P. West

Journal of the Arkansas Academy of Science

Tall fescue (Festuca arundinacea Schreb.) is the most commonly grown cool season grass used for pastures in Arkansas. Most tall fescue contains a fungal endophyte (Acremonium coenophialum Morgan-Jones & Gams), which causes fescue toxicosis in livestock and costs cattle producers millions of dollars annually in lost production. Endophyte presence is known to reduce wild mammal populations in areas where tall fescue is prevalent. The endophyte spends its entire life cycle within the plant and is transmitted through the seed. The association is mutualistic with the plant providing nutrients for the endophyte and the endophyte conferring drought, insect, and nematode resistance …

Evaluation Of Aposphaeria Amaranthi As A Bioherbicide For Pigweed (Amaranthus Spp.), A. S. Mintz, G. J. Weidmann

Journal of the Arkansas Academy of Science

Studies were conducted to determine the potential of the fungus, Aposphaeria amaranth!, as a bioherbicide for pigweeds (Amaranthus spp.). Experiments to establish the environmental parameters necessary for control of tumble pigweed (A. albus) demonstrated that an 8-hr dew period was sufficient for control of seedlings with four to six leaves, and that temperatures ranging from 20 to 28 C were conducive for disease development. Conidial concentrations as lowas 1x 10s conidia per ml also were sufficient for plant mortality. Host range tests demonstrated pathogenicity of A. amaranthi to several other species of Amaranthus, including biotypes resistant to triazine herbicides. Disease …

Origin And Evolution Of Defective Interfering Rnas Of Tomato Bushy Stunt Virus, David A. Knorr, Thomas Jack Morris

School of Biological Sciences: Faculty Publications

Viruses with defective genomes have been identified in association with virtually every major family of viruses and have been widely utilized as tools for investigating virus functions in animal cell culture systems (Perrault, 1981). It is generally thought that defective interfering viruses (DIs) arise through deletion, rearrangement, or recombination of a competent viral genome. DIs tack the ability for independent existence relying on their parental helper viruses to supply factors required for replication, maturation, and/or encapsidation (Huang and Baltimore, 1977). The interference attributed to DIs is thought to result from competition with the helper virus for factors required in trans …

Bulletin 4133 - Fungal Diseases Of Pasture Legumes In Western Australia, M. J. Barbetti

Bulletins 4000 -

Subterranean clover (Trifolium subterraneum L.) is the most important annual pasture legume in Western Australia, forming the basis of some 6.5 million ha of annual pastures. The success of subterranean clover may be attributed to its ability to increase soil fertility as a consequence of nitrogen fixed, to withstand heavy grazing, to control soil erosion and, with some cultivars, to tolerate waterlogging. Fungal diseases have been a major limitation to the productivity of subterranean clover, affecting both the amount of plant matter and the quantity of seed produced.

Annual medic (Medicago spp., in particular M. polymorpha L.) is also an …

Breeding Phomopsis-Resistant Lupins, John Sylvester Gladstones

Journal of the Department of Agriculture, Western Australia, Series 4

The release of Gungurru and Yorrel lupins marks the coming of age of the narrow-leafed lupin as a crop plantt. These are the first cultivars of the species th have substantial resistance to Phomopsis stem blight, which will make the stubbles much safer for grazing stock.

This article describes the new lupins, their background, and how they were bred. It also acknowledges the contributions of colleagues who helped in important ways.

Symptoms And Treatment Of Copper Deficiency Of Wheat, J W. Gartrell, R. F. Brennan, A. D. Robson

Journal of the Department of Agriculture, Western Australia, Series 4

Copper deficiency in wheat is still common but may not be easily noticed.

Bulletin No 3573 - Wheat Diseases In Western Australia, A. G. P. Brown, G. C. Mac Nish

Bulletins - 3000 - 3999

Diseases caused by pathogenic organisms can seriously affect the yield of wheat. Some diseases are comparatively rare whereas others occur over a large area of the wheatbelt year after year.

it is important to be able to identify diseases correctly so that the appropriate control measures can be applied, In the following article the diseases of wheat caused by pathogenic organisms are illustrated, with their symptoms described, and where possible, the control measures given.

Bulletin No 3863 - Plants Which Cause Kimberley Horse Disease, A. L. Payne

Bulletins - 3000 - 3999

Crotalaria crispata and Crotalaria retusa (Rattlepod), two plants responsible for Kimberley horse disease*, are, unfortunately, fairly widespread throughout the Kimberleys. Both species are apparently toxic in all stages of growth.

This bulletin is to assist pastoralists to:-

• Identify the two plants;

• Recognise the type of country and conditions under which they grow; and

• Locate horse paddocks on country where the plants are least likely to occur.

Wheat Rust Epidemics, A G P Brown, J. T. Reeves

Journal of the Department of Agriculture, Western Australia, Series 4

Stem rust of wheat is probably the most damaging plant disease in the world. The rust fungus attacks stems, leaves and heads, producing characteristic red-brown pustules which affect the plant's ability to fill out the grain. Yield loss is directly related to the amount of rust in the crop and the length of time the crop is infected.

In Western Australia it is not uncommon to find rust on crops in mid-October, but usually there is negligible loss of yield. In fact this pattern is quite normal throughout the world: In general, rust arrives too late to cause an epidemic. …

Annual ('Wimmera') Ryegrass Toxicity, Geoffrey A. Pearce, O. M. Goss, P. H. Berry, J. L. Wise

Journal of the Department of Agriculture, Western Australia, Series 4

Because annual ryegrass is one of the most widespread pasture plants in Western Australia, annual ryegrass toxicity is a threat to livestock in many agricultural areas—even though the area now affected is small.

The problem is being intensively studied by the Department of Agriculture and a working group has been set up to investigate potential control measures.

In this article the group reviews the problem and reports progress in its investigations to the end of 1973.

Wimmera Ryegrass Toxicity, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

Wimmera ryegrass toxicity has been reported for many years to affect sheep and occasionally cattle in New Zealand, South Australia and the United States of America.

In Western Australia, occasional reports since 1959 and severe outbreaks of staggers in the Katanning- Gnowangerup areas over the last two years prompted the investigation reported below.

Bulletin No 3768 - Submitting Plants For Disease Identification, M D. Marcley

Bulletins - 3000 - 3999

THERE are several important points to remember when submitting plant material for disease identification by the Department of Agriculture's Plant Pathology Branch.

Firstly it is very important to collect a suitable sample because it is very difficult to determine the cause of a plant disease by looking at a completely dead plant. The sample should include the entire plant (before it is completely dead) including roots and soil. Then all the effects of the disease can be seen. For example leaf yellowing, dead areas of the stem, collar rot, root rot, eelworm damage, and disease of the water and food …

Submitting Plants For Disease Identification, M D. Marcley

Journal of the Department of Agriculture, Western Australia, Series 4

THERE are several important points to remember when submitting plant material for disease identification by the Department of Agriculture's Plant Pathology Branch.

Lupins In Western Australia. 5. The Grazing Value Of Green And Mature Lupins, John Sylvester Gladstones

Journal of the Department of Agriculture, Western Australia, Series 4

WHETHER or not lupins are grown primarily as a grain crop, grazing of standing crops and harvested stubbles will continue to be an important use. This article examines the uses of sweet lupins for forage, and the main problem of lupin grazing, lupinosis.

Oat Diseases In Western Australia, W R. Tweedie, W. A. Shipton

Journal of the Department of Agriculture, Western Australia, Series 4

OVER ONE MILLION acres of oats are grown yearly in this State, making the crop next in importance to wheat.

Pathogenic organisms are responsible for considerable reductions in yield, yet losses can be minimized if control measures are taken.

Cyanogenetic Plants Of Western Australia, T E H Aplin

Journal of the Department of Agriculture, Western Australia, Series 4

THIS article deals with the more important cyanogenetic plants found in Western Australia.

It also deals with plants which have not been proved as cyanogenetic but which do produce toxic effects similar to those produced by cyanogenetic plants.

Symptoms, post mortem appearances and the treatment for affected stock are also dealt with.

Supplementary List Of Disease Recorded On Various Hosts In Western Australia, G C. Macnish

Journal of the Department of Agriculture, Western Australia, Series 4

THIS supplement contains the diseases recorded on all hosts during the period July 1, 1961 and June 30, 1963 inclusive.

A few disease records omitted from previous census have been incorporated. A list of previous publications in this series is appended. Also appended is a list of common names of host plants to facilitate reference.

A Study Of Puccinia Graminis And Cronartium Ribicola, Duane W. Hughes

Graduate Student Research Papers

It was the purpose of this study (1) to review the literary history of rusts in the United States and in the Northwest in particular, ( 2) to learn where rusts generally are found geographically, (3) to gain further information regarding the etiology of wheat rust (Puccinia graminis tritici), of white pine blister rust (Cronartium ribicola) and to become enlightened on other rusts, (4) to learn ways of controlling the two rusts, black stem rust of wheat and white pine blister rust, and to gain an appreciation of the economic importance of rust prevention.

List Of The Smut Fungi Of Arkansas, George E. Templeton

Journal of the Arkansas Academy of Science

No abstract provided.

Chemical Poisoning In Animals. 3. Prussic Acid, H D. Seddon

Journal of the Department of Agriculture, Western Australia, Series 4

HYDROCYANIC or prussic acid poisoning is a serious and not uncommon ailment of ruminants, and may be a cause of rapid death when pasture plants capable of developing dangerous levels of the poison are being grazed.

Bulletin 121 - Mildew, Septoria, Leaf Spots And Similar Diseases Of Cereals, W. M. Carne, J. G. C. Campbell

Bulletins 1 - 2999

Mildew attacks all the cereals as well as many grasses. but as in black stem rust the fungus has developed specialised races, one attacking wheat, another barley, another rye and so on, so that the form attacking wheat will not attack oats, etc