Plant Breeding and Genetics Commons^™

Identification Of Qtl Modifying The Activity Of The Tcb1-S Locus And Characterization And Sequencing Of Two Plutonium-Beryllium Induced Reduced Gametophyte Transmission Mutants In Maize, Merritt Bryer Burch

Electronic Theses and Dissertations

This thesis is split into two independent projects both involving the male gametophyte generation of maize. The first project looks at how pollen interacts with the female gametophyte to reduce its transmission in cross-incompatible reactions controlled by the unilateral cross-incompatibility system, teosinte crossing barrier 1. The second project explores two plutonium-beryllium induced male gametophyte mutants and attempts to uncover their genetic basis.

Identification of QTL Modifying the Activity of the Tcb1-s Locus
Teosinte crossing barrier 1 (Tcb1) is a unilateral cross-incompatibility system present in maize that provides a pre-zygotic pistil barrier to plants carrying Tcb1-s (strong allele) from pollen …

Intraspecific Genetic Variation, Population Structure, And Performance Of The Invasive Aquatic Macrophyte Eurasian Watermilfoil (Myriophyllum Spicatum) In Waterbodies With And Without Histories Of Chemical Herbicide Treatment Across Michigan, Taylor Zallek

Dissertations, Master's Theses and Master's Reports

Populations of invasive species are often subjected to novel selective forces in the form of anthropogenic control agents in their introduced ranges. These control agents, applied unevenly among populations within a species’ new range, can send invasive populations on drastically different evolutionary and ecological trajectories. In these studies, we aimed to see if different histories of chemical herbicide treatment are differentially influencing the genetic diversity, structure, and performance of populations of invasive watermilfoil (Myriophyllum spicatum and M. spicatum x M. sibiricum hybrids) from waterbodies in the state of Michigan. We sampled ten waterbodies with different histories of herbicide treatment …

Low- Copy Nuclear Markers In Isoëtes (Isoëtaceae) Identified With Transcriptomes, Peter W. Schafran, Gabriel Johnson, W. Carl Taylor, Elizabeth A. Zimmer, Lytton J. Musselman

Biological Sciences Faculty Publications

PREMISE OF THE STUDY: Few genetic markers provide phylogenetic information in closely related species of Isoëtes (Isoëtaceae). We describe the development of primers for several putative low- copy nuclear markers to resolve the phylogeny of Isoëtes, particularly in the southeastern United States.

METHODS AND RESULTS: We identified regions of interest in Isoëtes transcriptomes based on low- copy genes in other plants. Primers were designed for these regions and tested with 16 taxa of Isoëtes and one species of Lycopodium. Parts of the pgiC, gapC, and IBR3 gene regions show phylogenetic signal within the North American and …

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

This paper reports on six bicolor sugar-enhanced or synergistic sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

This paper reports on fifteen bicolor, two yellow, and two white supersweet sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Midwest Vegetable Trial Report For 2017, Elizabeth Maynard, Brad R. Bergefurd, Wenjing Guan, Petrus Langenhoven

Purdue Fruit and Vegetable Research Reports

This is a compilation of 19 research trial reports from six land-grant universities in the Midwestern United States. Crops include green beans, cantaloupe, cucumber, ornamental corn, pepper, potato, pumpkin, butternut squash, sweet corn, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties. One report addressed specific growing practices: plant spacing for sweet corn.

Tomato Cultivar Evaluation In High Tunnels, Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

Tomatoes are a common crop grown in unheated greenhouses, commonly called high tunnels. Production in these structures has increased in recent years. This trial was conducted to evaluate varieties for their performance as a summer-harvested crop in soil-based high tunnel production systems. Five hybrid determinate varieties or line, one hybrid indeterminate, and one open-pollinated indeterminate variety were compared for yield, earliness, fruit size, and quality.

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

This paper reports on six bicolor sugar-enhanced or synergistic sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Tomato Cultivar Evaluation In High Tunnels, Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

Tomatoes are a common crop grown in unheated greenhouses, commonly called high tunnels. Production in these structures has increased in recent years. This trial was conducted to evaluate varieties for their performance as a summer-harvested crop in soil-based high tunnel production systems. Five hybrid determinate varieties or line, one hybrid indeterminate, and one open-pollinated indeterminate variety were compared for yield, earliness, fruit size, and quality.

Pumpkin Variety Performance With And Without Treatment For Powdery Mildew In Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

Pumpkins are grown on 5,000 acres in Indiana with an average yield of 6.75 tons per acre. Successful pumpkin production requires the use of varieties that yield well and produce pumpkins of the size, shape, color, and quality demanded by the market. Genetic resistance to the fungal disease powdery mildew is present in some varieties. This trial was designed to evaluate performance of pumpkin varieties in northern Indiana with and without treatment for powdery mildew. The trial included fourteen orange jack-o-lantern size pumpkins, one small or 'pie' pumpkin and one specialty yellow pumpkin.

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

This paper reports on fifteen bicolor, two yellow, and two white supersweet sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Pumpkin Variety Performance With And Without Treatment For Powdery Mildew In Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

Pumpkins are grown on 5,000 acres in Indiana with an average yield of 6.75 tons per acre. Successful pumpkin production requires the use of varieties that yield well and produce pumpkins of the size, shape, color, and quality demanded by the market. Genetic resistance to the fungal disease powdery mildew is present in some varieties. This trial was designed to evaluate performance of pumpkin varieties in northern Indiana with and without treatment for powdery mildew. The trial included fourteen orange jack-o-lantern size pumpkins, one small or 'pie' pumpkin and one specialty yellow pumpkin.

Midwest Vegetable Trial Report For 2017, Elizabeth Maynard, Brad R. Bergefurd, Wenjing Guan, Petrus Langenhoven

Midwest Vegetable Trial Reports

This is a compilation of 19 research trial reports from six land-grant universities in the Midwestern United States. Crops include green beans, cantaloupe, cucumber, ornamental corn, pepper, potato, pumpkin, butternut squash, sweet corn, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties. One report addressed specific growing practices: plant spacing for sweet corn.

An Efficient Protocol For Root Studies In The Common Sunflower Using Composite Plants, Tyler Parks

Masters Theses

Sunflower, Helianthus annuus, is one of the most important oilseed crop in the world. Oil from the seeds is prized for its' exceptional quality and flavor. Despite this, sunflowers have been forced onto marginal lands often on semi-arid and non-fertile soil, due to disease pressures and economics making it critical to produce more resilient lines and varieties to withstand these new stresses. The recent availability of the sunflower genome can allow genome-wide characterization of gene families. Stable transformation protocols, which can be used for characterization studies, have been developed for H. annuus using Agrobacterium tumefaciens, but they produce …

Investigation Of A Putative Membrane-Bound Protein In Arabidopsis Thaliana And Nicotiana Tabacum, Allen Potter

Masters Theses

The NLG (Novel Leaf Gene) is a recently discovered gene from aspen (Populus tremula x alba). Previous research indicates that NLG impacts leaf shape. Our preliminary analyses indicate that NLG may function as a membrane bound protein with possible localization in plasma membrane and/or nucleus. Sequence and phylogenetic analyses in Arabidopsis thaliana and Populus trichocarpa identified four homologous genes in Arabidopsis and five in Populus. Gene expression analysis was done using online tools and indicated that NLG is expressed in multiple tissue types but predominately in stem and flowers. The protein coding region of the NLG orthologs genes from aspen …

Quantitative And Molecular Analysis Of Habituation At The Maize R1 Locus, Robert C. Lindsay

Theses and Dissertations

Epigenetics is the study of heritable changes in phenotypes that are not the result of changes in DNA sequence. Examples of epigenetic affecters include methylation changes, chromatin modifications, transcription factors, and RNA-based changes. The molecular mechanisms behind epigenetic changes are not fully understood. Canalization is the buffering of gene expression against environmental changes over time, while habituation is semi-stable expression change over time due to selection. This work characterized the molecular changes associated with the kernel color changes of the R-sc:86-17pale allele at the maize red color1 (r1) locus to determine if the changes are epigenetic in nature. …

Natural And Anthropogenic Drivers Of Tree Evolutionary Dynamics, Brandon M. Lind

Theses and Dissertations

Species of trees inhabit diverse and heterogeneous environments, and often play important ecological roles in such communities. As a result of their vast ecological breadth, trees have become adapted to various environmental pressures. In this dissertation I examine various environmental factors that drive evolutionary dynamics in threePinusspecies in California and Nevada, USA. In chapter two, I assess the role of management influence of thinning, fire, and their interaction on fine-scale gene flow within fire-suppressed populations of Pinus lambertiana, a historically dominant and ecologically important member of mixed-conifer forests of the Sierra Nevada, California. Here, I find evidence …

Morphological, Genetic And Biochemical Characterization Of The Anti-Malarial Herb, Artemisia Annua Germplasm Collection At West Virginia University, Delini K. Samarasinghe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Malaria is one of the deadliest diseases in human history. Nearly half of the world’s population, is at the risk in 106 countries. Only in 2016, this disease killed about 445,000 people, 72% of them being children under age five. It also accounts for US $12 billion dollars of direct costs in Africa alone. Five different species of Plasmodium cause malaria but P. falciparum is the most detrimental one, causing 50% of all malaria cases and is considered as the deadliest parasite in humans. Artemisinin (ART), a 15 C sesquiterpenoid is currently the only precursor to the most effective anti-malarial …