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2023

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Articles 1681 - 1693 of 1693

Full-Text Articles in Plant Biology

Sequencing Antisense Transcriptome Of Populus Through Antisense/Sense Transcript Pair Enrichment, Emma V. Burke Jan 2023

Sequencing Antisense Transcriptome Of Populus Through Antisense/Sense Transcript Pair Enrichment, Emma V. Burke

Dissertations, Master's Theses and Master's Reports

Past transcriptome research on plants focused primarily on protein coding genes, and only recently researchers began looking into the non-protein coding regions that may play significant roles in gene regulation. Antisense RNA transcripts that are found naturally in the cell share complementary sequence with sense transcripts and have been shown to regulate expression of their sense counterparts. Since antisense RNA has been largely under-studied and difficult to sequence because of their low relative abundance, new methods are needed to target antisense RNA for efficient genome-wide profiling. To address this gap in methods to efficiently and cost effectively enrich antisense RNA …


Effect Of Soil Ph And Indigenous Bacterial Populations In Selecting Rhizobial Endophytes Within Soybean Root Nodules, Brianne Danielle Edwards Jan 2023

Effect Of Soil Ph And Indigenous Bacterial Populations In Selecting Rhizobial Endophytes Within Soybean Root Nodules, Brianne Danielle Edwards

Graduate Theses/Dissertations

Soybean plants fulfill most of their nitrogen requirement by developing symbiotic associations with four different rhizobial genera, including Bradyrhizobium, Sinorhizobium, Mesorhizobium, and genus Rhizobium. In general, members of Bradyrhizobium and Sinorhizobium have been reported as dominant root nodule endophytes under acidic soils and alkaline soil conditions, respectively. So far, it is unknown whether the selection of rhizobial endophytes is regulated by their ability to survive under different soil pH or driven by host plants regardless of their relative abundance in soil. This study was focused on the assessment of the potential role of soil pH in …


Morphological And Genetic Analysis Of The Root System In Two American Grapevines (Vitis Species), Parinaz Mohtasebi Jan 2023

Morphological And Genetic Analysis Of The Root System In Two American Grapevines (Vitis Species), Parinaz Mohtasebi

Graduate Theses/Dissertations

The North American grapes species Vitis rupestris Scheele and Vitis riparia Michx have been the pillars of rootstock breeding for many decades. Though a large body of viticultural knowledge has been accumulated on their impact on grafted scions, the genetic basis of their root system architecture (RSA) has received limited scientific attention. In this study, I generated and analyzed adventitious root systems from dormant cuttings of 22 V. riparia and 19 V. rupestris accessions, as well as 162 interspecific F1 hybrid progeny from a cross between V. rupestris (♀) and V. riparia (♂). I photographed the roots and then extracted …


Correction To: Genetic Characterization And Expression Analysis Of Wheat (Triticum Aestivum) Line 07or1074 Exhibiting Very Low Polyphenol Oxidase (Ppo) Activity, S. M. Hystad, J. M. Martin, R. A. Graybosch, M. J. Giroux Jan 2023

Correction To: Genetic Characterization And Expression Analysis Of Wheat (Triticum Aestivum) Line 07or1074 Exhibiting Very Low Polyphenol Oxidase (Ppo) Activity, S. M. Hystad, J. M. Martin, R. A. Graybosch, M. J. Giroux

Department of Agronomy and Horticulture: Faculty Publications

The above mentioned article was published in 2015 with an error in the naming of one of the allele sequences for the PPO gene on the D genome. The PPO-D1f allele was incorrectly named as it is identical to the PPO-D1c allele previously published in GenBank by the authors on March 11th, 2014. An alignment between the previously published PPOD1c allele and a resequencing of the parental line 07OR1074 shows that the PPO-D1f allele is identical to the PPO-D1c allele. The allele in the paper referred to as PPO-D1f should be treated as PPO-D1c and can be accessed at NCBI …


Estimating The Genetic Parameters Of Yield-Related 1 Traits Under Different Nitrogen Conditions In Maize, Semra Palali Delen, Gen Xu, Jenifer Velazquez-Perfecto, Jinliang Yang Jan 2023

Estimating The Genetic Parameters Of Yield-Related 1 Traits Under Different Nitrogen Conditions In Maize, Semra Palali Delen, Gen Xu, Jenifer Velazquez-Perfecto, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

Understanding the genetic basis responding to nitrogen (N) fertilization in crop production is a long-standing research topic in plant breeding and genetics. Albeit years of continuous efforts, the genetic architecture parameters, such as heritability, polygenicity, and mode of selection, underlying the N responses in maize remain largely unclear. In this study, about n = 230 maize inbred lines were phenotyped under high N (HN) and low N (LN) conditions for two consecutive years to obtain six yield-related traits. Heritability analyses suggested that traits highly responsive to N treatments were less heritable. Using publicly available SNP genotypes, the genome-wide association study …


Zonal Application Of Plant Growth Regulator In Cotton To Reduce Variability And Increase Yield In A Highly Variable Field, Carlos M.P. Vaz, Júlio C. Franchini, Eduardo A. Speranza, Ricardo Y. Inamasu, Lúcio A. De C. Jorge, Ladislau M. Rabello, Ivani De O.N. Lopes, Sérgio Das Chagas, Jefferson L.R. De Souza, Márcio De Souza, Amandio Pires, James Schepers Jan 2023

Zonal Application Of Plant Growth Regulator In Cotton To Reduce Variability And Increase Yield In A Highly Variable Field, Carlos M.P. Vaz, Júlio C. Franchini, Eduardo A. Speranza, Ricardo Y. Inamasu, Lúcio A. De C. Jorge, Ladislau M. Rabello, Ivani De O.N. Lopes, Sérgio Das Chagas, Jefferson L.R. De Souza, Márcio De Souza, Amandio Pires, James Schepers

Department of Agronomy and Horticulture: Faculty Publications

Variable-rate application has great potential to reduce variability and increase yield by spatially optimizing agricultural inputs. In cotton, plant growth regulators (PGRs) control excessive growth and provide suitable plant height for harvest operations. This study evaluates the effect of variable-rate PGR application compared to constant-rate application to reduce yield spatial variability and increase yield. The variable-rate approach was carried out in 2020 based on zonal applications defined by clustering analysis using soil electrical conductivity, vegetation indexes, and yield maps. Application doses and timings were determined by integrating plant height measurements for the whole field in 2019 and by zone in …


Interaction Of Quizalofop-P-Ethyl With 2,4-D Choline And/Or Glufosinate For Control Of Volunteer Corn In Corn Resistant To Aryloxyphenoxypropionates, Mandeep Singh, Vipan Kumar, Stevan Z. Knezevic, Suat Irmak, John L. Lindquist, Santosh Pitla, Amit J. Jhala Jan 2023

Interaction Of Quizalofop-P-Ethyl With 2,4-D Choline And/Or Glufosinate For Control Of Volunteer Corn In Corn Resistant To Aryloxyphenoxypropionates, Mandeep Singh, Vipan Kumar, Stevan Z. Knezevic, Suat Irmak, John L. Lindquist, Santosh Pitla, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Corn resistant to aryloxyphenoxypropionates (FOPs) (Enlist™ corn) enables the use of quizalofop-p-ethyl (QPE) as a selective postemergence (POST) herbicide for control of glufosinate/glyphosate-resistant corn volunteers. Growers usually mix QPE with 2,4-D choline and/or glufosinate to achieve broad-spectrum weed control in Enlist™ corn. The objectives of this study were to (1) evaluate the efficacy of QPE applied alone or mixed with 2,4-D choline and/or glufosinate for control of glufosinate/glyphosate-resistant corn volunteers in Enlist™ corn and (2) determine the impact of application time (V3 or V6 growth stage of volunteer corn) of QPE-based treatments on volunteer corn control as well as Enlist™ …


The Biggest Grower: A Youth Gardening Competition For Growing Specialty Crops And Urban Farmers, Stacy A. Adams, Terri James Jan 2023

The Biggest Grower: A Youth Gardening Competition For Growing Specialty Crops And Urban Farmers, Stacy A. Adams, Terri James

Department of Agronomy and Horticulture: Faculty Publications

Youth today have tendencies for unhealthy lifestyles, being sedentary, consuming high fat diets low in fruits and vegetables, all contributing to child obesity, Type 2 diabetes, hypertension, and precedence for life-long health concerns. For rural and economically disadvantaged urban households, poor diet is directly attributed to the inaccessibility of fresh produce, affordability and understanding of preparation of various specialty crops. Through a grant from the NE Specialty Crop Block Funding Program, The Biggest Grower Competition (BGC) was developed as a summer extension education program for youth in 8th through 12th grade. Project goals included; provide educational materials on …


Propagation, Physiology And Biomass Of Giant Cane (Arundinaria Gigantea) For Conservation And Restoration, Sanjeev Sharm Jan 2023

Propagation, Physiology And Biomass Of Giant Cane (Arundinaria Gigantea) For Conservation And Restoration, Sanjeev Sharm

Graduate Theses/Dissertations

Giant cane (Arundinaria gigantea) is a native species to 22 states in the U.S. The species and its ecosystem are considered critically endangered, and the species has been reduced to 2% of its original extent. The species has a long cultural and conservation history. Large canebrakes were commonly found in Missouri in bottomland forests, stream and riverbanks, and margins of lakes. My research goals were to: 1) examine methods for propagation success from field to greenhouse to field; 2) examine the physiology of cane at one of the few current canebrakes, for greenhouse propagated plants, and field planted …


Natural Transmission Of Grapevine Vein Clearing Virus, Matthew Manu Jan 2023

Natural Transmission Of Grapevine Vein Clearing Virus, Matthew Manu

Graduate Theses/Dissertations

More than 60 million tons of grapes are produced annually in the world, making them one of the most widely grown fruit crops. Despite grapes’ economic and health benefits, biotic stressors, such as viruses, cause significant loss to the grape and wine industry. One such virus is grapevine vein clearing virus (GVCV) which seriously threatens grape cultivation in the Midwest region of the United States. This virus has caused the removal of seven commercial vineyards since its discovery in 2004. About 34% of Ampelopsis cordata wild vines are infected with GVCV and serve as a primary inoculum for the spread …


Winter Dynamics Of Storm Water Management Ponds And Winter Tolerance In Three Aquatic Plant Species, Patrick Strzalkowski Jan 2023

Winter Dynamics Of Storm Water Management Ponds And Winter Tolerance In Three Aquatic Plant Species, Patrick Strzalkowski

Theses and Dissertations (Comprehensive)

The vast majority of the research into the performance of stormwater management ponds (SWMPs) has been performed in warm regions or during the warmer seasons in temperate regions. It is presumed that SWMPs are inactive in the winter as any potential stormwater is trapped in snow and ice. The main goal of this thesis was to test this presumption and to study the dynamics and performance of three SWMPs during the winter. Remote water level loggers were installed into the three SWMPs and daily grab samples from the influents and effluents were taken and analyzed for total phosphorus (TP), chloride, …


Natural Variation In Temperature-Modulated Immunity To Pseudomonas Syringae Pv. Tomato (Pst Dc3000) In Arabidopsis Thaliana Plants, Christina A. M. Rossi Jan 2023

Natural Variation In Temperature-Modulated Immunity To Pseudomonas Syringae Pv. Tomato (Pst Dc3000) In Arabidopsis Thaliana Plants, Christina A. M. Rossi

Theses and Dissertations (Comprehensive)

Studies conducted on the model plant-pathogen system Arabidopsis thaliana-Pseudomonas syringae pv. tomato (Pst) showed that elevated temperature suppresses the pathogen-induced production of salicylic acid (SA), which is a key plant defence hormone. This increased host vulnerability at higher temperatures is due to downregulated expression of CALMODULIN BINDING PROTEIN 60-LIKE G (CBP60g) and SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1), which encode master regulators of SA production. Collectively, these previous studies only focused on one natural accession of Arabidopsis called Columbia-0 (Col-0). However, the intraspecific variation in Arabidopsis immunity phenotypes under elevated temperature remains unknown. To fill this critical knowledge gap, my …


Investigation Of The Function Of Protein Acyl Transferases Using Crispr-Cas9 To Create Null Mutants, Claire E. Christopher Jan 2023

Investigation Of The Function Of Protein Acyl Transferases Using Crispr-Cas9 To Create Null Mutants, Claire E. Christopher

Honors Theses and Capstones

24 Protein Acyl Transferases (PATs) have been identified in the model organism Arabidopsis thaliana. Despite knowing the enzymatic function of these genes (palmitoylation), the specific subcellular pathways, protein targets, and cellular activities of these proteins remain unknown. To investigate the pathways that PAT genes are involved in, deletion mutations were created in several genes and the phenotype of the organisms carrying homozygous mutations was observed. To introduce mutations, the CRISPR/Cas9 system was inserted into the plant’s genome using Agrobacterium tumefaciens to cause deletions in PAT genes of interest. To date, no CRISPR-induced pat mutants have a noticeable phenotype. These …