Plant Biology Commons^™

Site Population Trial Data, Margaret E. Maloney, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Chemical Analysis Data, Charlotte A. Shade, Kevin W. Custer, Julia I. Chapman, Ryan W. Mcewan

Data Files

Hardness, Alkalinity, Orthophosphate, Soluble reactive phosphorus, Total suspended solids, Dissolved organic carbon, Total Kjeldahl nitrogen, Nitrate:Nitrite ratio

Aquatic Fine Woody Debris, Erin C. Rowekamp, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Prototype Data, Margaret E. Maloney, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Lonicera Maackii Leaf Feeding Tests: Initial Mass, Kevin W. Custer, Julia I. Chapman, Ryan W. Mcewan

Data Files

Lymnaea stagnalis, Hyalella Azteca, and Allocapnia spp.

Lonicera Maackii Leaf Feeding Tests: Physicochemical Data, Kevin W. Custer, Julia I. Chapman, Ryan W. Mcewan

Data Files

Lymnaea stagnalis, Hyalella Azteca, and Allocapnia spp.

Lonicera Maackii Flower Leachate Toxicity: Survival And Mass, Kevin W. Custer, Julia I. Chapman, Ryan W. Mcewan

Data Files

In-stream (in situ) and in-lab microcosm trials using Hyalella Azteca and Anthopotamus verticis

Leaf Litter Basket, Erin C. Rowekamp, Taylor M. Buskey, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Seedling, Erin C. Rowekamp, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Aquatic Coarse Woody Debris, Erin C. Rowekamp, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Biofilm Ash Free Dry Mass And Chlorophyll A, Kevin W. Custer, Sean D. Mahoney, Mitchell J. Kukla, Corey Kuminecz, Joseph Murphy, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Managing Wicked Herbicide-Resistance: Lessons From The Field, Jill Schroeder, Michael Barrett, David R. Shaw, Amy B. Asmus, Harold Coble, David Ervin, Raymond A. Jussaume Jr., Michael D. K. Owen, Ian Burke, Cody F. Creech, A. Stanley Culpepper, William S. Curran, Darrin M. Dodds, Todd A. Gaines, Jeffrey L. Gunsolus, Bradley D. Hanson, Prashant Jha, Annie E. Klodd, Andrew R. Kniss, Ramon G. Leon, Sandra Mcdonald, Don W. Morishita, Brian J. Schutte, Christy L. Sprague, Phillip W. Stahlman, Larry E. Steckel, Mark J. Vangessel

Department of Agronomy and Horticulture: Faculty Publications

Herbicide resistance is ‘wicked’ in nature; therefore, results of the many educational efforts to encourage diversification of weed control practices in the United States have been mixed. It is clear that we do not sufficiently understand the totality of the grassroots obstacles, concerns, challenges, and specific solutions needed for varied crop production systems. Weed management issues and solutions vary with such variables as management styles, regions, cropping systems, and available or affordable technologies. Therefore, to help the weed science community better understand the needs and ideas of those directly dealing with herbicide resistance, seven half-day regional listening sessions were held …

Growth, Fitness, And Overwinter Survival Of A Shattercane (Sorghum Bicolor Ssp. Drummondii) × Grain Sorghum (Sorghum Bicolor Ssp. Bicolor) F2 Population, Jared J. Schmidt, Melinda K. Yerka, Jeffrey F. Pedersen, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Although sorghum [Sorghum bicolor (L.) Moench ssp. bicolor] is the fifth most important grain crop in terms of global production, no commercial hybrids carry genetically engineered (GE) traits for resistance to insect pests or herbicides due to regulatory concerns about gene flow to weedy relatives. However, non-GE herbicide resistance currently is being developed in grain sorghum and will likely transfer to related weeds. Monitoring the impact of this new nuclear technology on the evolution and invasiveness of related weeds requires a baseline understanding of the population biology of grain sorghum genes once they transfer to in situ weed …

Genomic Selection In Preliminary Yield Trials In A Winter Wheat Breeding Program, Vikas Belamkar, Mary J. Guttieri, Waseem Hussain, Diego Jarquin, Ibrahim El-Basyoni, Jesse Poland, Aaron J. Lorenz, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Genomic prediction (GP) is now routinely performed in crop plants to predict unobserved phenotypes. The use of predicted phenotypes to make selections is an active area of research. Here, we evaluate GP for predicting grain yield and compare genomic and phenotypic selection by tracking lines advanced. We examined four independent nurseries of F_3:6 and F_3:7 lines trialed at 6 to 10 locations each year. Yield was analyzed using mixed models that accounted for experimental design and spatial variations. Genotype-by-sequencing provided nearly 27,000 high-quality SNPs. Average genomic predictive ability, estimated for each year by randomly masking lines as missing …

Investigating The Ecology Of A Rare Species On St. John, Usvi: Reintroducing Solanum Conocarpum In Light Of Climate Change, Cecilia Rogers

Senior Honors Projects, 2010-2019

Approximately two thirds of St. John is National Park territory. However, the land has been threatened with tourism and development, greatly impacting island biodiversity. One species that may become extinct due to this degradation is Solanum conocarpum. S. conocarpum is a rare shrub, endemic to the dry tropical forests of St. John, USVI. This plant is a species of conservation concern and is one of very few native and endemic plants on this island. Very little is known about the ecology and reproduction of S. conocarpum. Most plants are found on the southern half of the island. Recent observations …

The Role Of Environmental Filtering In Structuring Appalachian Tree Communities: Topographic Influences On Functional Diversity Are Mediated Through Soil Characteristics, Julia I. Chapman, Ryan W. Mcewan

Biology Faculty Publications

Identifying the drivers of community assembly has long been a central goal in ecology, and the development of functional diversity indices has provided a new way of detecting the influence of environmental gradients on biotic communities. For an old-growth Appalachian forest, we used path analysis to understand how patterns of tree functional diversity relate to topography and soil gradients and to determine whether topographic effects are mediated through soil chemistry. All of our path models supported the idea of environmental filtering: stressful areas (high elevation, low soil moisture, low soil nutrients) were occupied by communities of low functional diversity, which …

Tomato Spotted Wilt Virus Nss Protein Supports Infection And Systemic Movement Of A Potyvirus And Is A Symptom Determinant, Hernan Garcia-Ruiz, Sergio M. Gabriel Peralta, Patricia A. Harte-Maxwell

Department of Plant Pathology: Faculty Publications

Plant viruses are inducers and targets of antiviral RNA silencing. To condition susceptibility, most plant viruses encode silencing suppressor proteins that interfere with antiviral RNA silencing. The NSs protein is an RNA silencing suppressor in orthotospoviruses, such as the tomato spotted wilt virus (TSWV). The mechanism of RNA silencing suppression by NSs and its role in virus infection and movement are poorly understood. Here, we cloned and tagged TSWV NSs and expressed it from a GFP-tagged turnip mosaic virus (TuMV-GFP) carrying either a wild-type or suppressor-deficient (AS9) helper component proteinase (HC-Pro). When expressed in cis, NSs restored pathogenicity and promoted …

Intermolecular Rna Recombination Occurs At Different Frequencies In Alternate Forms Of Brome Mosaic Virus Rna Replication Compartments, Hernan Garcia-Ruiz, Arturo Diaz, Paul Ahlquist

Department of Plant Pathology: Faculty Publications

Positive-strand RNA viruses replicate their genomes in membrane-bound replication compartments. Brome mosaic virus (BMV) replicates in vesicular invaginations of the endoplasmic reticulum membrane. BMV has served as a productive model system to study processes like virus-host interactions, RNA replication and recombination. Here we present multiple lines of evidence showing that the structure of the viral RNA replication compartments plays a fundamental role and that recruitment of parental RNAs to a common replication compartment is a limiting step in intermolecular RNA recombination. We show that a previously defined requirement for an RNA recruitment element on both parental RNAs is not to …

Metagenomic Analysis Of Viruses Associated With Maize Lethal Necrosis In Kenya, Mwathi Jane Wamaitha, Deepti Nigam, Solomon Maina, Francesca Stomeo, Anne Wangai, Joyce Njoki Njuguna, Timothy A. Holton, Bramwel W. Wanjala, Mark Wamalwa, Tanui Lucas, Appolinaire Djikeng, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Background: Maize lethal necrosis is caused by a synergistic co-infection of Maize chlorotic mottle virus (MCMV) and a specific member of the Potyviridae, such as Sugarcane mosaic virus (SCMV), Wheat streak mosaic virus (WSMV) or Johnson grass mosaic virus (JGMV). Typical maize lethal necrosis symptoms include severe yellowing and leaf drying from the edges. In Kenya, we detected plants showing typical and atypical symptoms. Both groups of plants often tested negative for SCMV by ELISA. Methods: We used next-generation sequencing to identify viruses associated to maize lethal necrosis in Kenya through a metagenomics analysis. Symptomatic and asymptomatic leaf samples …

Susceptibility Genes To Plant Viruses, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Plant viruses use cellular factors and resources to replicate and move. Plants respond to viral infection by several mechanisms, including innate immunity, autophagy, and gene silencing, that viruses must evade or suppress. Thus, the establishment of infection is genetically determined by the availability of host factors necessary for virus replication and movement and by the balance between plant defense and viral suppression of defense responses. Host factors may have antiviral or proviral activities. Proviral factors condition susceptibility to viruses by participating in processes essential to the virus. Here, we review current advances in the identification and characterization of host factors …

Profile Of Genetically Modified Plants Authorized In Mexico, Mayra Teresa Garcia Ruiz, Aaron N. Knapp, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Mexico is a center of origin for several economically important plants including maize, cotton, and cocoa. Maize represents more than a food crop, has been declared a biological, cultural, agricultural and economic patrimony, and is linked to the national identity of Mexicans. In this review, we describe the historic and current use of genetically modified plants in Mexico and factors that contributed to the development of the biosafety regulation. We developed a database containing all permit applications received by the government to release genetically modified plants. A temporal and geographical analysis identified the plant species that have been authorized for …

Genomic-Enabled Prediction Models Using Multi-Environment Trials To Estimate The Effect Of Genotype × Environment Interaction On Prediction Accuracy In Chickpea, Manish Roorkiwal, Diego Jarquin, Muneendra K. Singh, Pooran M. Gaur, Chellapilla Bharadwaj, Abhishek Rathore, Reka Howard, Samineni Srinivasan, Ankit Jain, Vanika Garg, Sandip Kale, Annapurna Chitikineni, Shailesh Tripathi, Elizabeth Jones, Kelly R. Robbins, José Crossa, Rajeev K. Varshney

Department of Agronomy and Horticulture: Faculty Publications

Genomic selection (GS) by selecting lines prior to field phenotyping using genotyping data has the potential to enhance the rate of genetic gains. Genotype × environment (G × E) interaction inclusion in GS models can improve prediction accuracy hence aid in selection of lines across target environments. Phenotypic data on 320 chickpea breeding lines for eight traits for three seasons at two locations were recorded. These lines were genotyped using DArTseq (1.6 K SNPs) and Genotyping-by-Sequencing (GBS; 89 K SNPs). Thirteen models were fitted including main effects of environment and lines, markers, and/or naïve and informed interactions to estimate prediction …

Gene Expression Profiling Of Iron Deficiency Chlorosis Sensitive And Tolerant Soybean Indicates Key Roles For Phenylpropanoids Under Alkalinity Stress, Brian M. Waters, Keenan Amundsen, George L. Graef

Department of Agronomy and Horticulture: Faculty Publications

Alkaline soils comprise 30% of the earth and have low plant-available iron (Fe) concentration, and can cause iron deficiency chlorosis (IDC). IDC causes soybean yield losses of $260 million annually. However, it is not known whether molecular responses to IDC are equivalent to responses to low iron supply. IDC tolerant and sensitive soybean lines provide a contrast to identify specific factors associated with IDC.We used RNA-seq to compare gene expression under combinations of normal pH (5.7) or alkaline pH (7.7, imposed by 2.5mM bicarbonate, or pH 8.2 imposed by 5mM bicarbonate) and normal (25μM) or low (1μM) iron conditions from …

Morphological, Genetic And Biochemical Characterization Of The Anti-Malarial Herb, Artemisia Annua Germplasm Collection At West Virginia University, Delini K. Samarasinghe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Malaria is one of the deadliest diseases in human history. Nearly half of the world’s population, is at the risk in 106 countries. Only in 2016, this disease killed about 445,000 people, 72% of them being children under age five. It also accounts for US $12 billion dollars of direct costs in Africa alone. Five different species of Plasmodium cause malaria but P. falciparum is the most detrimental one, causing 50% of all malaria cases and is considered as the deadliest parasite in humans. Artemisinin (ART), a 15 C sesquiterpenoid is currently the only precursor to the most effective anti-malarial …

Pushing The Resolution Limit By Correcting The Ewald Sphere Effect In Single-Particle Cryo-Em Reconstructions, Dongjie Zhu, Xiangxi Wang, Qianglin Fang, James L. Van Etten, Michael G. Rossmann, Zihe Rao, Xinzheng Zhang

Department of Plant Pathology: Faculty Publications

The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a “block-based” reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes.

Genome-Wide Analysis Of Genes Encoding Core Components Of The Ubiquitin System In Soybean (Glycine Max) Reveals A Potential Role For Ubiquitination In Host Immunity Against Soybean Cyst Nematode, Chunyu Zhang, Li Song, Mani Kant Choudhary, Bangjun Zhou, Guangchao Sun, Kyle C. Broderick, Loren Giesler, Lirong Zeng

Department of Plant Pathology: Faculty Publications

Background: Ubiquitination is a major post-translational protein modification that regulates essentially all cellular and physiological pathways in eukaryotes. The ubiquitination process typically involves three distinct classes of enzymes, ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2) and ubiquitin ligase (E3). To date, a comprehensive identification and analysis of core components comprising of the whole soybean (Glycine max) ubiquitin system (UBS) has not been reported.

Results: We performed a systematic, genome-wide analysis of genes that encode core members of the soybean UBS in this study. A total of 1431 genes were identified with high confidence to encode putative soybean UBS components, …

Tor-Autophagy Branch Signaling Via Imp1 Dictates Plant-Microbe Biotrophic Interface Longevity, Guangchao Sun, Christian Elowsky, Gang Li, Richard Wilson

Department of Plant Pathology: Faculty Publications

Like other intracellular eukaryotic phytopathogens, the devastating rice blast fungus Magnaporthe (Pyricularia) oryzae first infects living host cells by elaborating invasive hyphae (IH) surrounded by a plant-derived membrane. This forms an extended biotrophic interface enclosing an apoplastic compartment into which fungal effectors can be deployed to evade host detection. M. oryzae also forms a focal, plant membrane-rich structure, the biotrophic interfacial complex (BIC), that accumulates cytoplasmic effectors for translocation into host cells. Molecular decision-making processes integrating fungal growth and metabolism in host cells with interface function and dynamics are unknown. Here, we report unanticipated roles for the M. …

Determination Of Vegetative Development And Flower Effect Of Transgenic Nicotiana Tabacum, Hasan Pinar, Aydin Uyun, Semih Yilmaz, Nedim Mutlu, Amitava Mitra

Department of Plant Pathology: Faculty Publications

Species and varieties in the Nicotiana genus may respond differently to photoperiodism in terms of flowering time control. These are classified as short day, neutral day and long day plants. One of these, Nicotiana tabacum cv. The Xanthi nc is a genotype which can flowering on a long day. But the genotype Kanamycin resistance can open its flowers in January when the resistance gene is transferred to the plant. On the other hand, the effect of rootstock to scion in plant species is very important. One of the issues raised is whether the transgenic plant in the rootstock is effect …

Genome-Wide Association Study For Identification And Validation Of Novel Snp Markers For Sr6 Stem Rust Resistance Gene In Bread Wheat, Amira M. I. Mourad, Ahmed Sallam, Vikas Belamkar, Stephen N. Wegulo, Robert Bowden, Yue Jin, Ezzat Mahdy, Bahy Bakheit, Atif A. El-Wafaa, Jesse Poland, Peter S. Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Stem rust (caused by Puccinia graminis f. sp. tritici Erikss. & E. Henn.), is a major disease in wheat (Triticum aestivium L.). However, in recent years it occurs rarely in Nebraska due to weather and the effective selection and gene pyramiding of resistance genes. To understand the genetic basis of stem rust resistance in Nebraska winter wheat, we applied genome-wide association study (GWAS) on a set of 270 winter wheat genotypes (A-set). Genotyping was carried out using genotyping-by-sequencing and ~35,000 high-quality SNPs were identified. The tested genotypes were evaluated for their resistance to the common stem rust race in …

Crop Residue Harvest Impacts Wind Erodibility And Simulated Soil Loss In The Central Great Plains, Yuxin He, Deann R. Presley, John Tatarko, Humberto Blanco-Canqui

Department of Agronomy and Horticulture: Faculty Publications

Crop residue removal can affect the susceptibility to soil wind erosion in climates such as those of the Central Great Plains, United States. Six on-farm trials were conducted in Kansas from 2011 to 2013 to determine the effects of winter wheat (Triticum aestivum L.), corn (Zea mays L.), and grain sorghum (Sorghum bicolor (L.) Moench), residue removal at 0, 25, 50, 75, and 100% of initial height on soil wind erosion parameters. Those parameters include soil surface random roughness (RR), and wind erodible fraction (EF; aggregates -¹ for three hours. Threshold wind velocity required to initiate …