Plant Biology Commons^™

Insect Infestation Of Maize, Zea Mays (L.) In Indigenous Storage Structures In Morogoro Region, Tanzania, Cp Rugumamu

Tanzania Journal of Science

Insect infestations and losses of maize stored in four types of indigenous storage structures in Morogoro Region, Tanzania were investigated. Infestation levels and weight losses caused by the larger grain borer, Prostephanus truncatus (Horn) and the maize weevil, Sitophilus zeamais (Motsch.) were found to differ significantly in the four types of storage structures (Dari, Kihenge, Kichanja and Reli). Lower infestation levels by the two pests and less weight losses were observed inDari and Kihenge than in Kichanja and Reli. The infestations and weight losses exhibited a significant trend that was in the order, Dari< Kihenge < Kichanja < Reli. These findings suggest that Dari and Kihenge are more appropriate for the incorporation in Integrated Pest Management (IPM) for P. truncatus and S. zeamais in Morogoro Region.

Concentrations Of Metals In River Sediment And Wetland Vegetations In Mining, Lake Victoria Basin, Tanzania, Jf Machiwa

Tanzania Journal of Science

Levels of metals were determined in river sediment, rice and sugarcane juice from Lake Victoria basin where small-scale gold processing activities are carried out to assess levels of contamination. Concentrations of metals in river sediments were generally high in areas that were closest to gold ore processing sites. For instance, mercury was highest (36.3 ±12.8 mg/g dw) in river sediments of Tigiti River in Mugumu District. Other heavy metals (Cd, Pb, Zn and Cu) had highest concentrations (2.20±0.71, 208±19.3, 412±20.1 and 132.7±11.9mg/g dw, respectively) in sediment samples from Kagota. The concentration of arsenic was highest (985.0±67.2 mg/g dw) in Mugusu …

News From Cart, Donald Padgett

Bridgewater Review

No abstract provided.

Gaca, The Response Regulator Of A Two-Component System, Acts As A Master Regulator In Pseudomonas Syringae Pv. Tomato Dc3000 By Controlling Regulatory Rna, Transcriptional Activators, And Alternate Sigma Factors, Asita Chatterjee, Yaya Cui, Hailian Yang, Alan Collmer, James R. Alfano, Arun K. Chatterjee

Department of Plant Pathology: Faculty Publications

Concerted investigations of factors affecting host-pathogen interactions are now possible with the model plant Arabidopsis thaliana and its model pathogen Pseudomonas syringae pv. tomato DC3000, as their whole genome sequences have become available. As a prelude to analysis of the regulatory genes and their targets, we have focused on GacA, the response regulator of a two-component system. The DC3000 gene was cloned by testing for the reversal of phenotypes of an Erwinia GacA– mutant. A GacA– mutant of DC3000 constructed by marker exchange produces much-reduced levels of transcripts of three alternate sigma factors: HrpL, required for the production of effector …

Activation Of A Coi1-Dependent Pathway In Arabidopsis By Pseudomonas Syringae Type Iii Effectors And Coronatine, Ping He, Satya Chintamanani, Zhongying Chen, Lihuang Zhu, Barbara N. Kunkel, James R. Alfano, Xiaoyan Tang, Jian-Min Zhou

Center for Plant Science Innovation: Faculty Publications

Gram-negative bacteria use a variety of virulence factors including phytotoxins, exopolysaccharides, effectors secreted by the type III secretion system, and cell-wall-degrading enzymes to promote parasitism in plants. However, little is known about how these virulence factors alter plant celluar responses to promote disease. In this study, we show that virulent Pseudomonas syringae strains activate the transcription of an Arabidopsis ethylene response factor (ERF) gene, RAP2.6, in a coronatine insensitive 1 (COI1)-dependent manner. A highly sensitive RAP2.6 promoter-firely luciferase (RAP2.6-LUC) reporter line was developed to monitor activities of various bacterial virulence genes. Analyses of P. syringae pv. tomato DC3000 mutants indicated …

Fisheries Research Contract Report No. 6 - Assessment Of The Nutritional Variability Of Lupins As An Aquaculture Feed Ingredient, Brett Glencross, John Curnow, Wayne Hawkins

Fisheries Research Reports

The nutritional value of the kernel meals of three species of lupin (Lupinus albus, L. angustifolius and L. luteus) was compared against each other and a reference ingredient of solvent extracted soybean meal, when fed to rainbow trout (Oncorhynchus mykiss). Each lupin kernel meal was included in test diets at 300 g/kg and also at a greater level, representing 50% of the total protein content in the diet. The digestibility of protein of all lupin kernel meals was better than for the soybean meal. The highest protein digestibility was that from L. luteus kernel meal at 30% inclusion (100%), which …

Bulletin No. 38: The Hidden World Of Plants: A Scanning Electron Microscope Survey Of The Native Plant Collection, Connecticut College Arboretum, Danica C. Kubick, T. Page Owen Jr.

Bulletins

No abstract provided.

Integrated Weed Management Plan For The Lower Las Vegas Wash, Elizabeth Bickmore

Publications (WR)

The Las Vegas Wash (Wash) is the primary drainage for the metropolitan Las Vegas Valley, eventually finding its way into the Colorado River watershed system. Pursuant to the recommendations of the Water Quality Citizens Advisory Committee, the Las Vegas Wash Coordination Committee (LVWCC) was formed in 1998 to develop a comprehensive plan for the long-term stabilization and management of the Wash. Consisting of representatives from 28 government agencies, businesses, environmental groups and citizens, the LVWCC formulated the Las Vegas Wash Comprehensive Adaptive Management Plan (CAMP) and designated the Southern Nevada Water Authority (SNWA) as the lead agency for the implementation …

Effects Of Modification Of The Transcription Initiation Site Context On Citrus Tristeza Virus Subgenomic Rna Synthesis, Maria A. Ayllon, Siddarame Gowda, Satyanarayana Tatineni, Alexander V. Karasev, Scott Adkins, Munir Mawassi, Jose Guerri, Pedro Moreno, William O. Dawson

Department of Plant Pathology: Faculty Publications

Citrus tristeza virus (CTV), a member of the Closteroviridae, has a positive-sense RNA genome of about 20 kb organized into 12 open reading frames (ORFs). The last 10 ORFs are expressed through 3'-coterminal subgenomic RNAs (sgRNAs) regulated in both amounts and timing. Additionally, relatively large amounts of complementary sgRNAs are produced. We have been unable to determine whether these sgRNAs are produced by internal promotion from the full-length template minus strand or by transcription from the minus-stranded sgRNAs. Understanding the regulation of 10 sgRNAs is a conceptual challenge. In analyzing commonalities of a replicase complex in producing so many …

Sheep Updates 2003 - Pastures, Ed Barrett-Lennard, Hayley Norman, Robyn Dynes, David Masters, David Henry, Stephen Gherardi, Graham Donald, Asoka Edirisinghe, Chris Oldham, Richard Smith, Joanne Sneddon, Mike Hyder, Andrew Thompson, Kazue Tanaka, Roy Latta, Chris Matthews, Brad Nutt, Angela Loi, Tim Wiley

Sheep Updates

This session covers seven papers from different authors: 1. Pastures for saline land, Ed Barrett-Lennard 1Department of Agriculture, Western Australia, Hayley Norman, Robyn Dynes and David Masters CSIRO Livestock Industries, Centre for Mediterranean Agricultural Research, Floreat Park, 2. Feeding value - the essential link between pastures and animals CSIRO Livestock Industries, Centre for Environmental and Life Sciences 3. ‘Pastures from space’ - how do we do it, how well do we do it and what do producers think about it? Stephen Gherardi Department of Agriculture Western Australia Graham Donald Asoka Edirisinghe Dave Henry CSIRO Livestock Industries Chris Oldham Department of …

Heritability Of Cooking Time And Water Absorption Traits In Dry Beans (Phaseolus Vulgaris L.) Using A North Carolina Design Ii Mating Scheme, Fm Elia

Tanzania Journal of Science

Estimation of genetic variances, heritabilities and estimations of response to selection of the cooking time and water absorption traits of Andean gene pool of dry bean seed Phaseolus vulgaris L. were done. General combining ability (GCA) i.e. GCA males and GCA females, and Specific combining ability (SCA) were estimated. The parents were crossed in a North Carolina mating design II and genetic analysis was made on the F3 and F4. Both male and female effectations within sets for the cooking trait in F3 and F4 were highly significant for the traits studied. Variances due to General combining ability (GCA) and …

Induced Resistance As A Mechanism Of Biological Control By Lysobacter Enzymogenes Strain C3, Ozlem Kilic-Ekici, Gary Y. Yuen

Department of Plant Pathology: Faculty Publications

Induced resistance was found to be a mechanism for biological control of leaf spot, caused by Bipolaris sorokiniana, in tall fescue (Festuca arundinacea) using the bacterium Lysobacter enzymogenes strain C3. Resistance elicited by C3 suppressed germination of B. sorokiniana conidia on the phylloplane in addition to reducing the severity of leaf spot. The pathogen-inhibitory effect could be separated from antibiosis by using heat-inactivated cells of C3 that retained no antifungal activity. Application of live or heat-killed cells to tall fescue leaves resulted only in localized resistance confined to the treated leaf, whereas treatment of roots resulted in …

A Bt Transgene Reduces Herbivory And Enhances Fecundity In Wild Sunflowers, A. A. Snow, Diana Pilson, L. H. Rieseberg, M. J. Paulsen, N. Pleskac, M. R. Reagon, D. E. Wolf, S. M. Selbo

Diana Pilson Publications

Gene flow from transgenic crops can introduce novel traits into related species, but the ecological importance of this process is unknown. Here, we report the first empirical evidence that wild plants can benefit from a bacterial transgene under uncaged, natural conditions. Cultivated sunflower (Helianthus annuus) is known to hybridize frequently with wild sunflower (H. annuus) in the western and midwestern United States. We studied a crop-developed Bacillus thuringiensis (Bt) transgene, cry1Ac, in backcrossed wild sunflower populations. Lepidopteran damage on transgenic plants was strongly reduced relative to control plants at our two study sites, while damage …

Crop Updates - 2003 Weeds, Bill Roy, Peter Newman, J. R. Peirce, B. J. Rayner, Kathryn J. Steadman, Gavin P. Bignell, Amanda J. Ellery, Sandy Nedelkos, Ross Chapman, Michelle J. Owen, Simone Dudley, Robert Gallagher, Pippa J. Michael, Julie A. Plummer, Peter Newman, Glenn Adam, Catherine Borger, Abul Hashem, Nerys Wilkins, Sally Peltzer, Alex Douglas, Fran Hoyle, Paul Matson, Michael Walsh, Darren Chitty, David Ferris, Paul Neve, Art Diggle, Patrick Smith, Stephen Powles, David Archer, James Eklund, Frank Forcella, Alister Draper, Rick Llewellyn, Aik Cheam, Siew Lee, David Nicholson, Mike Clarke, Debbie Allen, Harmohinder S. Dhammu, Terry Piper, L. E. Young

Crop Updates

This session covers Thirty four papers from different authors

INTRODUCTION

INTEGRATED WEED MANAGEMENT

IWM system studies/demonstration sites

1. Six years of IWM investigation – what does it tell us? Bill Roy, Agricultural Consulting and Research Services Pty Ltd

2. Long term herbicide resistance site, the final chapter, Peter Newman and Glen Adam, Department of Agriculture

3. Management of skeleton weed (chondrilla juncea) in a cropping rotation in Western Australia, J. R. Peirce and B. J. Rayner, Department of Agriculture

WEED BIOLOGY AND COMPETITION

1. Annual ryegrass seedbanks: The good, the bad and the ugly, Kathryn J. Steadman¹, Amanda …

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Crop Updates - 2003 Oilseeds, Peter Nelson, Graham Walton, Hasan Zaheer, Paul Carmody, J. A. Fortescue, D. W. Turner, Janet Wroth, Wallace Cowling, Anh-Van Pham, R. F. Brennan, Glen Riethmuller, Greg Hamilton, Jo Hawksley, C. M. Zaicou-Kunesch, D. Eksteen, Françoise A. Berlandier, Christiaan Valentine, Owain Edwards, Roger Jones, Brenda Coutts, Lisa Smith, Jenny Hawkes, Ravjit Khangura, Moin Salam, Art J. Diggle, Martin J. Barbetti, Kevin Walden

Crop Updates

This session covers fifteen papers from different authors

ACKNOWLEDGMENTS

VARIETIES

1. Large scale canola varietal evaluation in WA, Peter Nelson, Oilseeds WA

2. Performance of IT and TT canola varieties in the medium and high rainfall agzones of WA 2001-02, Graham Walton, Hasan Zaheer and Paul Carmody, Department of Agriculture

QUALITY

1. Reproductive biology, cotyledon development and oil accumulation in canola, J.A. Fortescue and D.W. Turner, School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia

2. Plant and environmental factors affecting oil concentration in canola – a mini-review, D.W. Turner, School of Plant Biology, …

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Crop Updates 2003 - Cereals, Graham Crosbie, Robert Loughman, Collin Wellings, Greg Shea, Simon Mckirdy, Neil C. Turner, Brenda Shackley, Wal Anderson, Darshan Sharma, Mohammad Amjad, Steve Penny Jr, Melanie Kupsch, Anne Smith, Veronika Reck, Pam Burgess, Glenda Smith, Elizabeth Tierney, Peter Burges, Moin U. Salam, Megan Collins, Art Diggle, Blakely Paynter, Roslyn J. Jettner, Kevin Young, Jocelyn Ball, Natasha Littlewood, Lucy Anderton, Irene Waters, Tim Setter, Jeff Russell, Reg Lance, Chengdao Li, Sue Broughton, Michael Jones, Grace Zawko, Keith Gregg, Stephen Loss, Frank Ripper, Ryan Guthrie, Daniel Bell, Patrick Gethin, Narelle Hill, Laurence Caeslake, Vivien Vanstone, Sean Kelly, Helen Hunter, Christopher R. Newman

Crop Updates

This session covers twenty one papers from different authors:

PLENARY

1. Recognising and responding to new market opportunities in the grains industry, Graham Crosbie, Manager, Grain Products Research, Crop Breeding, Plant Industries, Department of Agriculture

2. Stripe rust – where to now for the WA wheat industry? Robert Loughman¹, Colin Wellings² and Greg Shea¹¹Department of Agriculture, ²University of Sydney Plant Breeding Institute, Cobbitty (on secondment from NSW Agriculture)

3. Benefits of a Grains Biosecurity Plan, Dr Simon McKirdy, Plant Health Australia, Mr Greg Shea, Department of Agriculture

4. Can we improve …

A Watershed Analysis Of Threemile Pond: Implications For Water Quality And Land Use Management, Colby Environmental Assessment Team, Colby College, Problems In Environmental Science Course (Biology 493), Colby College

Colby College Watershed Study: Threemile Pond (2003)

The 2003 Colby Environmental Assessment Team chose to study the Threemile Pond watershed. The Threemile Pond watershed is located in Vassalboro, China., Windsor, and Augusta, Maine. Threemile Pond is a popular site for recreation and is home to a wide range of flora and fauna. Like all other lakes in Maine, it is a young lake. However, intensive human activity in the watershed contributes a substantial amount of nutrients and the lake has algal blooms annually in the summer months. The purpose of this study was to assess the impact of land use and development on the water quality of …

A Utah Flora: 3rd Edition, Stanley L. Welsh, N. Duane Atwood, Sherel Goodrich, Larry C. Higgins

Books by Faculty of the Monte L. Bean Life Science Museum

No abstract provided.

On The Relationship Between Stomatal Characters And Atmospheric Co2, C. D. Reid, H. Maherali, H. B. Johnson, S. D. Smith, S. D. Wullschleger, R. B. Jackson

Life Sciences Faculty Research

Leaf stomatal characters influence the response of terrestrial evapotranspiration to climate change and are used as proxies for the reconstruction of past atmospheric [CO2]. We examined the phenotypic response of stomatal index (SI), density (SD) and aperture (AP) to rising atmospheric CO2 in 15 species after four years exposure to a field CO2 gradient (200 to 550 μmol mol−1 atmospheric [CO2]) or at three Free Air CO2 Enrichment (FACE) sites. Along the CO2 gradient, SI and SD showed no evidence of a decline to increasing [CO2], while AP decreased slightly. There was no significant change in SI, SD or AP …

The Pseudomonas Syringae Type Iii-Secreted Protein Hopptod2 Possesses Protein Tyrosine Phosphatase Activity And Suppresses Programmed Cell Death In Plants, Avelina Espinosa, Ming Guo, Vincent C. Tam, Zheng Qing Fu, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

The bacterial plant pathogen Pseudomonas syringae possesses a type III protein secretion system that delivers many virulence proteins into plant cells. A subset of these proteins (called Avr proteins) is recognized by the plant’s innate immune system and triggers defences. One defence-associated response is the hypersensitive response (HR), a programmed cell death (PCD) of plant tissue. We have previously identified HopPtoD2 as a type III secreted protein from P. s. pv. tomato DC3000. Sequence analysis revealed that an N-terminal domain shared homology with Avr- PphD and a C-terminal domain was similar to protein tyrosine phosphatases (PTPs). We demonstrated that purified …

Chitosanase May Enhance Anti-Fungal Defense Responses In Transgenic Tobacco, Bill L. Hendrix

Inquiry: The University of Arkansas Undergraduate Research Journal

Fungicides are expensive, dangerous, and can be harmful to the environment, but they are often necessary for profitable farming operations. New technologies may soon allow farmers to replace these chemicals with genetically engineered plants producing antifungal enzymes that degrade fungal cell walls. To explore this option, a Paenbacillus chitosanase gene was cloned, sequenced, and modified for plant expression. The modified gene was delivered to tobacco (Nicotiana tabacum L cv. Xanthine) leaf disks via Agrobacterium tumenfaciens-mediated transformation. The putative GMOs were tested for transgene integration, transcription, and translation. Confirmed transformants were then screened for enhanced responses to a Rhizoctonia solani cell …

The Role Of Natural Calcium Oxalate Crystals In Plant Defense Against Chewing Insects, Sarah J. Doege

Inquiry: The University of Arkansas Undergraduate Research Journal

Calcium oxalate is the most abundant insoluble mineral found in plants and it is common among many plant families. Calcium oxalate crystals in plants can appear as irregular rectangles, spiked balls, or needles. The formidable appearance of these crystals has lead to speculation that they might serve as a form of pest control by deterring chewing insects. We utilized mutant plant lines to assess the effects of plant calcium oxalate crystals on the survival and feeding habits of chewing insects. We have taken advantage of calcium oxalate-deficient (cod) mutants of the barrel medic, Medicago truncatula Calcium oxalate crystals accumulate in …

Forage Yield And Economic Losses Associated With The Brown-Midrib Trait In Sudangrass, M. D. Casler, Jeffrey F. Pedersen, D. J. Undersander

Department of Agronomy and Horticulture: Faculty Publications

Brown-midrib genes increase digestibility due to reduced lignification in sudangrass, Sorghum bicolor subsp. drummondii (Nees ex Steud.) de Wet & Harlan. Brown-midrib lines are known to be low in forage yield potential, but this reduction in forage yield has not been previously quantified. The objectives of this study were to quantify the increase in forage quality and decrease in forage yield and to provide an economic assessment of this dichotomy. Piper and Greenleaf (normal leaves) were compared with their brown-midrib counterparts and four highly selected brown-midrib (FG) lines at two locations for 2 yr. Brown-midrib lines averaged 9.0% lower in …

Registration Of Rn582 Sorghum Germplasm Line, J. F. Pedersen, J. J. Toy

Department of Agronomy and Horticulture: Faculty Publications

RN582 sorghum [Sorghum bicolor (L.)Moench] germplasm line (Reg. no. GP-591, PI 628277) was developed jointly by the USDA-ARS and the Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska, and was released in September 2001.

RN582 is an S₆ selection from the cross (RTx430 ms₃ms₃X E 35-1) X E 35-1. RTx430 was developed and released by the Texas Agricultural Experiment Station (Miller, 1984) and was provided to this project containing the nuclear male sterility gene ms₃ by A.B. Maunder in 1988. E 35-1 is an Ethiopian land race with white …

Diurnal Variation In The Basal Emission Rate Of Isoprene, Jennifer L. Funk, Clive G. Jones, Christine J. Baker, Heather M. Fuller, Christian P. Giardina, Manuel T. Lerdau

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Isoprene is emitted from numerous plant species and profoundly influences tropospheric chemistry. Due to the short lifetime of isoprene in the atmosphere, developing an understanding of emission patterns at small time scales is essential for modeling regional atmospheric chemistry processes. Previous studies suggest that diurnal fluctuations in isoprene emission may be substantial, leading to inaccuracies in emission estimates at larger scales. We examined diurnal patterns in the basal emission rate of isoprene in red oak (Quercus rubra), eastern cottonwood (Populus deltoides), and eucalyptus (Eucalyptus saligna) and the influence of light and temperature on the magnitude of these diurnal patterns. Maximum …

Fungal Biology And Agriculture: Revisiting The Field, O. Yarden, D. J. Ebbole, S. Freeman, R. J. Rodriguez, M. B. Dickman

Department of Plant Pathology: Faculty Publications

Plant pathology has made significant progress over the years, a process that involved overcoming a variety of conceptual and technological hurdles. Descriptive mycology and the advent of chemical plant-disease management have been followed by biochemical and physiological studies of fungi and their hosts. The later establishment of biochemical genetics along with the introduction of DNA-mediated transformation have set the stage for dissection of gene function and advances in our understanding of fungal cell biology and plant–fungus interactions. Currently, with the advent of high-throughput technologies, we have the capacity to acquire vast data sets that have direct relevance to the numerous …

Transcription Strategy In A Closterovirus: A Novel 5'-Proximal Controller Element Of Citrus Tristeza Virus Produces 5'- And 3'-Terminal Subgenomic Rnas And Differs From 3' Open Reading Frame Controller Elements, Siddarame Gowda, Maria A. Ayllon, Satyanarayana Tatineni, Moshe Bar-Joseph, William O. Dawson

Department of Plant Pathology: Faculty Publications

Citrus tristeza virus (CTV) produces more than thirty 3'- or 5'-terminal subgenomic RNAs (sgRNAs) that accumulate to various extents during replication in protoplasts and plants. Among the most unusual species are two abundant populations of small 5'-terminal sgRNAs of approximately 800 nucleotides (nt) termed low-molecular-weight tristeza (LMT1 and LMT2) RNAs. Remarkably, CTV replicons with all 10 3' genes deleted produce only the larger LMT1 RNAs. These 5'-terminal positive-sense sgRNAs do not have corresponding negative strands and were hypothesized to be produced by premature termination during plus-strand genomic RNA synthesis. We characterized a cis-acting element that controls the production …

Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman

The Journal of Undergraduate Research

HVCR21 is a barley protein known to be cold regulated at the mRNA level. However, its function as well as the affect of low temperature on its translation are unknown. The purpose of this project was to engineer E. coli to express recombinant HVCR21. PGR primers were designed for the 5' and 3' ends of the HVCR21 coding region. The primers were also designed with a 5' SacI restriction site and a 3' Pst I restriction site. After ligation of the PCR product into the pCR4-TOPO vector, bacteria were transformed and plated and the successful transformant verified by PCR. The …

Patterns Of Water Uptake And Rhizosphere Salinity In Casuarina Obesa Miq. During A Drying Period At Lake Toolibin, Western Australia, Patrick J. Mitchell

Theses : Honours

Lake Toolibin is one of a few remaining freshwater lakes in the central wheatbelt of Western Australia. Since monitoring began at Lake Toolibin in the early 1970's groundwater levels have risen to within 1-2 m of the lakebed with an accompanying migration of salt. The site-specific nature of water uptake in Casuarina obesa Miq. (the dominant lakebed tree species) and rhizosphere salinity were explored spatially through analyses of soil properties (groundwater depth and salinity, particle size and components of soil water potential ψ₁). Plant water sources (using δ²H signatures) and water availability (based on ψ_{leaf …}

Evaluation Of Resistance Screening Methods For Sclerotinia Stem Rot Of Soybean And Dry Bean, Linda S. Kull, Tri D. Vuong, Kris S. Powers, Kent M. Eskridge, James R. Steadman, Glen L. Hartman

Department of Plant Pathology: Faculty Publications

Three methods to identify levels of resistance to Sclerotinia sclerotiorum in soybean (Glycine max) and dry bean (Phaseolus vulgaris) were compared using multiple data analyses. The three methods were mycelial plug inoculations of cotyledons, cut stems, and detached leaves. Six S. sclerotiorum isolates of known relative aggressiveness were inoculated on each of three soybean and dry bean cultivars with varied response to S. sclerotiorum. For soybean, all three inoculation methods accurately identified isolate aggressiveness irrespective of cultivar, but identification of susceptible and partially resistant soybean cultivars was influenced by isolate. For dry bean, the cotyledon and cut …