Plant Biology Commons^™

Differential Gene Expression Of Ethylene Biosynthesis And Signaling Associated With Climacteric Behaviors In Indonesian Melon, Vida Rahma Latifah, Adib Fakhruddin Yusuf, Karso Suryo Putro, Dian Sartika, Anjar Tri Wibowo, Awik Puji Dyah Nurhayati, Karlia Meitha, Miftahudin Miftahudin, Budi Setiadi Daryono

Makara Journal of Science

Fruit ripening in melon (Cucumis melo L.) is a complex developmental process that is characterized by the coexistence of climacteric and non-climacteric varieties within the same species. This study characterized the ripening behavior of four local Indonesian melon cultivars (“Melona”, “GMP”, “Tacapa Gold”, and “Kinaya”) by integrating morphophysio-logical traits with transcriptional expression analysis of ethylene biosynthesis (CmACS, CmACO) and signaling (CmATH, CmEREBP) genes. The results revealed a strong agreement between the phenotypic traits and molecular regulation. “Melona” and “GMP” exhibited a strong climacteric profile, which was characterized by natural fruit abscission, sweet aroma …

Differential Effects Of Thiamine, Bap, And Thidiazuron Combinations On Shoot Multiplication And Phenolic-Flavonoid Accumulation In Pineapple In Vitro Cultures, Dinda Septia Rachman, Erni Suminar, Anne Nuraini, Fiky Yulianto Wicaksono, Suseno Amien, Syariful Mubarok, Hermanto

Jurnal Kultivasi

The demand for high-quality pineapple (Ananas comosus (L.) Merr.) planting materials continues to increase, while conventional propagation methods are limited in multiplication rate, uniformity, and production efficiency. In vitro culture offers an alternative approach for rapid and large-scale seedling production. However, information on the effects of thiamine and cytokinin combinations on both shoot multiplication and phenolic-flavonoid accumulation in pineapple in vitro remains limited. This study evaluated ten defined combinations of thiamine and cytokinin for shoot multiplication and total phenolic-flavonoid contents of pineapple explants. The experiment employed a completely randomized design with 10 treatment combinations of thiamine (0.1 and 0.4 mg/L), …

Performance Of Dual-Form Terminalia Catappa Organic Fertilizers On Sweet Corn Yield And Economic Viability In Tropical Highlands, Stefina Liana Sari, Eso Solihin, Rahmat Budiarto

Jurnal Kultivasi

Recycling underutilized plant biomass into organic fertilizers underpins low-input cropping strategies, but liquid and solid formulations derived from a common feedstock have seldom been evaluated side by side under field conditions. This study aimed to (i) characterize the physicochemical properties and safety profile of a liquid organic fertilizer (LOF) and a crumb-type organic fertilizer (COF) co-produced from Terminalia catappa leaf compost, (ii) compare their effects on sweet corn growth and yield, (iii) determine relative agronomic effectiveness (RAE), and (iv) evaluate economic feasibility of the most promising organic–inorganic combinations. We produced LOF and COF through aerobic composting of Terminalia catappa leaves …

Modeling The Clonal Rosette Composition Of A Bromeliaceae Genet: A Combinatorial Approach, Layla K. Lammers, Erin N. Bodine

Spora: A Journal of Biomathematics

Bromeliaceae, a neo-tropical plant family encompassing over 3,000 species, exhibit two modes of reproduction: sexual reproduction via flowers and seeds, and asexual reproduction via genetically identical clonal rosettes. The vegetative bodies of bromeliads form rosettes with new leaves emerging from the center and clonal rosettes emerging above a single leaf in the rosette, resulting in a genetic individual consisting of a seed-grown rosette and multiple iterations of clonal rosettes. This research develops a combinatorial model of probability that a single genetic individual will include at least n clonal rosettes when a single rosette can produce at most 1 or 2 …

Plant Diversity In Laos, And Its Strategic Significance For Building China-Laos Community With A Shared Future For Biodiversity, Meng Honghu, Bo Li, Wenbin Yu, Yaowu Xing

Bulletin of Chinese Academy of Sciences (Chinese Version)

Laos is the first country to sign the bilateral cooperation document for construction of the community with a shared future with China. Over the years, Laos and China have established clear directions for cooperation in politics, economy, security, culture, and ecology, opening a new chapter in the development of China-Laos relations. At present, global environmental governance is facing severe challenges, and the biodiversity loss has become a major international issue of growing concern. Nevertheless, biodiversity conservation and sustainable development remain relatively limited. Laos shares a boarder with China and is characterized by complex natural environments with rich plant resource. Yet, …

A Comparative Expression Analysis Of Selected Genes Involved In Secondary Metabolites Biosynthesis Among Three Alfalfa Cultivars Grown In Iraq, Izdehar Yahia Obaid, Bushra M. J. Alwash

Al-Bahir

Because of its abundance in proteins, vital amino acids, vitamins, and minerals that promote balanced nutrition, Medicago sativa L., also known as alfalfa, is regarded as a nutritionally excellent forage crop. In addition to its nutritional value, alfalfa produces a range of bioactive secondary metabolites, including flavonoids, alkaloids, and phenolic acids, further supporting its use in medicinal and nutritional applications. Three alfalfa cultivars grown in Iraq were examined for gene expression levels of the primary genes involved in secondary metabolite biosynthesis (PAL, COMT, CHS, and OMT). Among these cultivars are Victoria (VC), Green Gold (GC), and a local cultivar (LC). …

The Multifaceted Medicinal Properties Of Momordica Charantia: Insights Into Phytochemistry And Therapeutic Applications, Maham Ashfaq, Haroon Ur Rashid, Javaria Ashfaq, Shazia Ramzan, Fatma Hussain

Journal of Bioresource Management

Momordica charantia (bitter melon) is a significant medicinal plant, having been long used in traditional medicine in Asian, African and South American systems. M. charantia is more than a traditional medicine; by consolidating existing evidence, it is a promising source of drug discovery and can serve as a complementary in contemporary therapeutics. Over the last several decades, it has become the subject of contemporary biomedical studies due to its abundance of phytochemicals, such as saponins, flavonoids, alkaloids, and polypeptides. These bioactive compounds present a wide range of pharmacological processes that M. charantia can be a natural solution to some of …

Visualizing Ozone Pollution In The Denver Metro Area Using A Bioindicator Garden, Ella K. Mathews, Hillary Hamann, Julie Morris, Erica L. Larson

DU Undergraduate Research Journal Archive

Since the industrial revolution, widespread fossil fuel combustion has significantly increased atmospheric pollution. The combustion of fossil fuels releases nitrogen oxides (NOx) and volatile organic compounds (VOCs), which form tropospheric (ground-level) ozone when exposed to sunlight. Ozone directly harms plants by disrupting their ability to absorb carbon dioxide, release oxygen, and carry out photosynthesis. Bioindicator species are plants that have visible responses to environmental conditions, such as ozone, and can act as “canaries in the coal mine,” indicating the health of their environment. In this study, we used a garden of ozone bioindicator species (ozone garden) as a cost-effective tool …

Response Of Chlorophyll, Morphology, And Yield Of Several East Java Superior Soybean (Glycine Max L.) Varieties To Levels Of Salinity, Puji Lestari Tarigan, Ida Retno Moeljani, Fadila Suryandika, Hadi Munarko

Jurnal Kultivasi

Soybean production has been extended to saline areas. This development is a strategy to expand planting areas to suboptimal land to increase worldwide production. To support cultivation in these locations, it is necessary to develop superior varieties that are resistant to salinity and provide supporting technological inputs. In saline conditions, soybeans may face stress that leads to morphological, physiological, and yield disturbances. Consequently, the response of several soybeans in East Java should be observed. This study is a pot experiment and arranged in a Factorial Completely Randomized Design (CRD) consisting of two factors, soybean varieties and NaCl concentrations. The treatments …

Comparing The Effect Of Varying Concentrations Of Polytetrafluoroethylene On The Length And Fresh Weight Of Salvinia Minima, Talha Islam

Caroliniana Undergraduate Research Journal

The increasing usage of fluoropolymer pollution in aquatic environments has increased concerns about the ecological consequences of materials such as polytetrafluoroethylene (PTFE), commonly known as Teflon. In the past decade, the usage of Teflon in American homes across the United States has risen by 37.7%, resulting in large amounts of aquatic pollution due to improper disposal of these harmful substances (Török et al., 2020). Although PTFE is chemically inert, its accumulation in freshwater systems can introduce matter that physically interacts with aquatic organisms. This study examined how varying concentrations of PTFE suspensions affect the growth of Salvinia minima, a …

Impact Of One Nucleotide On Organ Enumeration And Phyllotaxy In Arabidopsis Thaliana, Hazel R. Frans, Tara Phelps-Durr

SACAD: Scholarly Activities

Arabidopsis thaliana is a model organism used by scientists to study plant genetics, development, and physiology. CRISPR-Cas9 is a biotechnology tool adapted from a bacterial defense mechanism to precisely edit DNA using a guide mRNA and a Cas9 protein. This project aims to create CRISPR-CAS9 mutations in the APETALA3 (AP3) gene of the model plant Arabidopsis thaliana. AP3 is a class B gene critical to the petal and stamen development of Arabidopsis flowers. The AP3 protein contains a MADS domain, which binds directly to DNA and may be responsible for the expression of the CaRG-box genes. AP3 works in conjunction …

Liparis Condylobulbon Reichb. F. From Gumitir, Indonesia: Morphology, Molecular, And Phytochemical Profile, Fuad Bahrul Ulum, Asyifa Yasmin Arum, Kurnia Sa’Adah, Dwi Setyati, Edia Fitria Dwinianti, Ranjithkumar Rajamani, Ari Satia Nugraha, Mukhamad Su’Udi

Makara Journal of Science

Liparis condylobulbon Rchb.f. is an epiphytic orchid across South and Southeast Asia, which holds notable ethnomedicinal and pharmacological significance. This study aims to characterize Indonesian Liparis condylobulbon using integrated morphological, molecular (matK, rbcL, ITS2), and phytochemical (LC-MS, DPPH) analyses of specimens from Mount Gumitir, providing the first comprehensive taxonomic and pharmacological profile to support species authentication and future bioprospecting. The L. condylobulbon specimen displays diagnostic epiphytic morpho-logy, including fusiform pseudobulbs, two elongate leaves, and an erect inflorescence bearing ~123 flowers. Molecular barcoding using matK, rbcL, and ITS2 confirmed its identity, with phylogenetic analyses consistently clustering it with …

Effects Of Phosphorus Addition On Fungal Colonization In Little Bluestem, Isabel M. Zanartu, Alexx Nicholas, Mckenzie Goodwyn, Connor Morozumi

The Cardinal Edge

Little Bluestem (Schizachyrium scoparium) is a North American perennial grass that is able to tolerate high heat and droughts. This is possible by its extensive underground root network that taps deep into the ground for resources. Within the same soil systems are fungal endophytes, which are symbionts that live within plant tissues including roots. Fungal endophytes can affect host growth and nutrient acquisition, but the functional role these endophytes play in plant health may be dependent on the abiotic conditions the plant is facing. In the modern era, one large driver of abiotic conditions is synthetic fertilizer use which alters …

Impact Of Legacy Amf Communities On Vegetative Growth & Yield Of Soybean, Dylan Habeeb, Connor Morozumi, Natalie Christian

The Cardinal Edge

Soil microbial communities are increasingly recognized as an important factor in sustainable agriculture. Arbuscular mycorrhizal fungi (AMF), are key players in these communities, known to benefit their plant host in a number of ways, such as increased nutrient uptake. Plants can partner with multiple AMF species; however, little is known about how interactions between multispecies communities of AMF impact plant growth and yield. In an agricultural setting, the long-term development of these communities following practices such as rotational planting or cover cropping is also an important consideration, but there is little research on the long-term legacy of AMF communities or …

Effects Of Salinity On Interactions Between Epichloë Amarillans And Fusarium Spp. In American Beachgrass, Emma G. Mills, Dylan Habeeb, Natalie Christian, Connor Morozumi

The Cardinal Edge

Effects of Salinity on interactions between Epichloë amarillans and Fusarium spp. In American Beachgrass

Emma Mills, Dylan Habeeb, Natalie Christian, Connor Morozumi

Epichloë is a well-known genus of vertically transmitted fungal endophytes in many species of grasses. In Ammophila breviligulata (American beachgrass), Epichloë amarillans is a key endophyte species known to cause chemical and morphological changes to its host and provide protection from stressors. Additionally, Epichloë is thought to influence fungal endophyte community composition within its host. However, deepening research on the interaction of Epichloë with coexisting fungal endophytes may shed further light on its role in community composition. Additionally, …

Asimina Triloba (L.) Dunal: The Complete Story Of America’S Forgotten Fruit, Autumn M. Howard

The Cardinal Edge

Asimina triloba, commonly known as the pawpaw, is a unique and historically significant native fruit tree of the eastern United States. This research paper explores the taxonomy, botanical description, distribution, habitat requirements, ecological interactions, economic importance, and reproductive biology of A. triloba. The species is distinguished by its tropical-flavored fruits, adaptation to temperate climates, and reliance on clonal reproduction and specific insect pollinators. While offering promising opportunities for sustainable agriculture and riparian restoration, A. triloba faces challenges related to limited seed dispersal, susceptibility to certain pests and diseases, and the presence of neurotoxic compounds. Despite its historical importance …

Biology, Phenology, And Host Range Of A Seed-Feeding Weevil, Ceutorhynchus Constrictus (Coleoptera: Curculionidae), A Biological Control Agent For Garlic Mustard, Alliaria Petiolata, In North America, Ghislaine Cortat, Hariet L. Hinz, Esther Gerber, Elizabeth J. Katovich, Mary E. Marek-Spartz, Roger L. Becker, Robert S. Bourchier, Danielle Fife

The Great Lakes Entomologist

Ceutorhynchus constrictus Marsham is a seed-feeding weevil and potential biocontrol agent of the invasive plant garlic mustard, Alliaria petiolata (Bieb.) Cavara & Grande (Brassicaceae), in North America. Ceutorhynchus constrictus is native to Europe, has a single generation per year and eggs are laid into developing seeds within the seedpods of bolting garlic mustard plants. Females use their prolonged rostrum to bore holes through seedpods into the developing seed, deposit a single egg and then cover the opening with secretion. Larvae feed on ripening seeds and progress through three instars before exiting seedpods in late June, to pupate and overwinter in …

Non-Native Seagrass, Halophila Stipulacea, Reaches Northern Caribbean, Elizabeth R. Whitman, Elizabeth W. Stoner, Stephanie K. Archer

Gulf and Caribbean Research

Halophila stipulacea, a seagrass native to the western Indian Ocean, has become an established invader throughout the western Atlantic, where it often outcompetes native seagrass species across diverse environmental conditions. Here, we report its presence in the northern Bahamas, ca. 2300 km northwest of the first Caribbean record in Grenada and 304 km northeast of the recent observation in Florida, USA. This range expansion represents the northernmost Caribbean record to date and highlights the species’ capacity for long-distance dispersal and continued spread within the region. Our observation underscores the need for coordinated monitoring efforts to track the trajectory of …

Growth Of Porang (Amorphopallus Muelleri) Seedlings As Influenced By Seed Maturity And The Presence Of Sarcotesta, Indri Fariroh, Restiani Sih Harsanti, Aminatun Nisa, Didik Pudji Restanto, Rizky Anugerah Putra

Jurnal Kultivasi

Porang seed maturity based its position on spadix position significantly impacts germination. The presence of the seed coat (sarcotesta) also influenced germination and initial seedling growth. This study aimed to explore the effect of seed maturity and the presence of sarcotesta to the seedling growth. The experiment was conducted from January to June 2023. A two factor Randomized Complete Block Design was employed, examining three maturity levels based on spadix position (physiological maturity seed from the tip spadix (red colour), half physiological maturity seed from the middle spadix (orange colour), and immature seed from the base spadix (green colour)), and …

Comparative Analysis Of Taproot And Fibrous Root System Microbiomes Under Flooding Stress, Natalie S. Kozlowski, Leighanna Jake

Humboldt Journal of Microbiology

There has been increased interest in understanding how the microbiomes of vascular plants respond to flooding stress and, many studies have focused on monocotyledonous plants with fibrous root systems. This leaves a gap in knowledge of dicotyledonous plants with taproot systems, as previous studies have been in the context of flooding to cropland affecting major food crops which are often monocotyledonous plants with fibrous root systems. Since the structural and functional differences between these root systems may influence microbial community stability and response to flooding conditions, a study looking at both is long overdue As such, this study aims to …

The Effects Of Plastic Mulch And Irrigation On Hemp Chemistry And Leaf Endophytes, Allison Rietman, Connor Morozumi, Robert C. Pearce, Natalie Christian

Journal of Agricultural Hemp Research

As an emerging crop, cultivation techniques for industrial hemp (Cannabis sativa L.) are still being developed and optimized. For example, irrigation and plastic mulching are some management practices that have been touted for their benefits in improving crop quality. Both techniques ensure ample water availability, which has often been attributed to improving hemp cannabinoid and terpene composition. In this study, we examined how supplemental irrigation implemented in a plastic mulch system influenced the cannabinoid and terpene profiles of one cultivar of CBD hemp, Lifter. We grew industrial hemp in field with plastic mulch and applied regular irrigation to one group …

Gama Melon Parfum, A Potential Melon Genotype Tolerant Of Salinity Stress, Giri Nugroho, Tatik Chikmawati, Dian Sartika, Adib Fakhruddin Yusuf, Karso Suryo Putro, Vida Rahma Latifah, Budi Setiadi Daryono, Anjar Tri Wibowo, Awik Puji Dyah Nurhayati, Karlia Meitha, Miftahudin Miftahudin

Makara Journal of Science

Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week …

The Effect Of Melatonin And 6-Benzylaminopurine Application On The Post-Harvest Quality Of Cut Roses (Rosa X Alba), Azizah Tiara Putri, Syariful Mubarok, Rahmat Budiarto

Jurnal Kultivasi

Roses are known as a high-value commodity frequently used in various important events. However, they are susceptible to postharvest quality deterioration, which can affect their vase life and appearance. In this study, roses with a blooming stage of approximately 25–50% were immersed in melatonin and 6-benzylaminopurine (BAP) solutions at different concentrations. This research aims to analyze the effect of melatonin and BAP application on the freshness of cut roses. The parameters observed included flower vase life, flower wilting angle, increase in flower diameter, fresh weight, solution uptake, and chlorophyll content. The results showed that melatonin and BAP, applied individually or …

Effect Of Preharvest Paclobutrazol And Nitrogen Fertilizers On The Sprouting Performance Of ‘Median’ Potato Seed G0 Tuber, Jajang Sauman Hamdani, Rahmat Budiarto, Anne Nuraini, Selika Fitrian Ramadani

Jurnal Kultivasi

Various intensifications of potato cultivation, such as the provision of paclobutrazol and nitrogen (N) fertilizer, are thought to impact the quality of the seeds produced. This study aims to evaluate the effects of different N fertilizer doses and paclobutrazol concentrations applied in the preharvest period on the sprouting performance of G0 potato tuber seeds after storage. Nine treatment combinations, each repeated three times, were tested, using 50%, 100%, and 150% of the recommended N dose and paclobutrazol concentrations of 50, 100, and 150 part per million (ppm), applied at 30 and 45 days after planting, respectively. The interaction effect between …

Effect Of Mercury Dose Variation On Growth And Nitrate Reductase Activity In Aquarius Palifolius (Nees & Mart.) Christenh. & Byng, Tsurayya Nurhanifah, Dwi Umi Siswanti

Makara Journal of Science

Mercury, a byproduct of mining, can harm the environment. One method to reduce this impact is through phytoremediation. This study used the Mexican sword (Aquarius palifolius), a plant with the potential to absorb and accumulate mercury. The aim of this study was to examine the effect of mercury stress on nitrate reductase activity, which plays a role in plant productivity, and to assess its impact on growth parameters, including plant height growth rate, leaf length, and number of leaves. Three-month-old A. palifolius plants obtained from rice fields in Sleman were treated with mercury at doses of 0, 14, …

Isolation And Enumeration Of Salmonella Enterica From Fresh Lettuce (Lactuca Sativa L.) Leaves And Its Susceptibility To Different Washing Protocols, Meinard Talplacido, Angella Melissa Carlos, Bryan Rey Oliveros, Ida Dalmacio

The Philippine Agricultural Scientist

Lettuce is one of the most consumed fresh produce items in the country, but it also serves as a vehicle for human pathogens, posing a risk of foodborne illness. This study used conventional microbiological and molecular analyses to obtain the concentration and prevalence of Salmonella enterica in fresh Green Ice and Iceberg lettuce leaves from selected supermarkets in Los Baños, Laguna, Philippines. The efficacy of different household washing procedures in reducing S. enterica on lettuce leaves was also investigated. Of the 19 composite Green Ice lettuce and 21 composite Iceberg lettuce samples tested, 5.26% and 4.76%, respectively, were contaminated with …

Effects Of Water Deficit Stress And Mycorrhiza, Azotobacter Chroococcum, And Azospirillum Lipoferum Biofertilizers On Yield And Morphological Traits Of Wheat (Triticum Aestivum L.) Cultivars, Mehdi Rashnou, Farshid Aref, Bahram Amiri, Masoud Rafiee, Mahdi Zare

The Philippine Agricultural Scientist

Crop production in various parts of Iran faces significant constraints due to insufficient water availability, which is a major challenge to agricultural productivity. To study the effects of water deficit stress on shoot and root growth characteristics and seed yield of different wheat (Triticum aestivum L.) cultivars, an experiment was conducted using a randomized complete block design with a split-plot arrangement and 3 replications. The main factor was irrigation treatments (full irrigation during the growing season [S1], water withholding after flowering [S2], and water withholding after grain filling at the milk stage [S3]). The secondary factors were wheat cultivars …

Evaluation Of Thermotherapy On Potato Tubers To Control Tuber-Borne Nematodes, Meloidogyne Spp, Wawan Kurniawan, Supramana, Abdul Munif, Giyanto

Jurnal Kultivasi

Root knot nematodes (Meloidogyne spp.) are significant plant-parasitic nematodes frequently transmitted through potato tubers and are a major factor contributing to the decline in both crop quality and yield. This study aims to evaluate the effectiveness of thermotherapy for eliminating Meloidogyne spp. from potato tubers and its impact on sprouting viability. Thermotherapy was conducted by immersing potato tubers and second-stage juveniles (J2) of Meloidogyne spp. in water at temperatures of 50 °C, 52.5 °C, 55 °C, and 60 °C for durations ranging from 5 to 75 minutes, depending on the treatment. Parameters observed included the mortality rate of Meloidogyne spp. …

The Effect Of Topping And Various Cytokinin-Based Plant Growth Regulators Applications On Immature Liberica Coffee Growth, Yudithia Maxiselly, Joshua Samuel, Cucu Suherman

Jurnal Kultivasi

The growth of Liberica coffee in the 2-year immature plant phase can be improved by applying cytokinin-based plant growth regulators (PGRs) and topping. Toppings applied to plants can inhibit apical dominance, while cytokinin PGRs such as 6-Benzylaminopurine (BAP) and coconut water can stimulate plant shoot growth. This research aims to determine the effect of topping and PGR application on the vegetative growth of Liberica coffee plants. This experiment was carried out at the Ciparanje experimental field, Universitas Padjadjaran, West Java, with an elevation of ± 750 meters above sea level from March to June 2024. The experimental design used a …

A Review On Detection Of Drought Stress In Tea Plants Through Morphological, Physiological, And Biochemical Approaches, Intan Ratna Dewi Anjarsari, Erni Suminar, Nabila Ragil Wiharti

Jurnal Kultivasi

Tea plants (Camellia sinensis) are important commodities with high economic value, but their production is greatly affected by environmental stresses such as drought and extreme temperatures. Global warming in recent years has led to extreme weather events and an increase in the earth's temperature, which also causes drought. Drought stress is one of the most significant abiotic factors affecting crop productivity. However, despite significant progress, there remains a notable gap in research, particularly the lack of integrated studies that combine morphological, physiological, and biochemical indicators for early and precise detection of drought stress in tea plants. To identify and mitigate …