Plant Biology Commons^™

Foliar Fungicide Application Timing For Disease Management, Stay Green, And Yield In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The objective of the trial was to compare foliar fungicides applied at different corn growth stages for gray leaf spot (GLS) and southern rust (SR) efficacy. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % organic matter, and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to GLS) was planted at approximately 34,000 seed/A on 26 May, which is later than normal, due to wet spring …

Foliar Fungicide Modes Of Action For Southern Rust Management, Push Lodging, And Yield In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The objective of the trial was to compare foliar fungicides with different modes of action for southern rust (SR) efficacy. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % organic matter, and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to gray leaf spot (GLS)) was planted at approximately 34,000 seed/A on 26 May. Eight treatments were arranged in a randomized complete block design with six …

Deposition Aid Adjuvants Plus A Fungicide For Southern Rust Management, Stay Green, Push Lodging, And Yield Comparisons In Field Corn In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The objective of the trial was to compare foliar fungicides for southern rust (SR) efficacy. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % OM and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to gray leaf spot (GLS)) was planted at approximately 34,000 seed/A on 26 May. Eleven treatments were arranged in a randomized complete block design with six replications. . . . Phytotoxicity was …

Root-Lesion Nematodes, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

Root-lesion nematodes, Pratylenchus spp., are among the most commonly encountered plant-parasitic nematodes, with one or more species occurring in almost every field. Their small size (0.4-0.7 mm long) relative to most other plant-parasitic nematodes enables them to survive in almost any soil texture. Their wide host range and distribution make them one of the most damaging nematodes worldwide, ranging from cool temperate to tropical environments. At least eight species of root-lesion nematodes have been associated with sunflower.

Root-Knot Nematodes, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

Root-knot nematodes, Meloidogyne spp., are some of the most damaging plant pathogens. Several of the most common species of root-knot nematodes have been confirmed to reproduce and cause damage on sunflower, including M. arenaria, M. hapla, M. incognita, M. javanica, and M. hispanica.

Meloidogyne spp. occur worldwide but are especially common in temperate, subtropical, and tropical regions. Countries where Meloidogyne spp. have been confirmed to affect sunflower include the United States and the African nations of Egypt, Mozambique, South Africa, and Zambia. Root-knot nematodes tend to cause the most damage to plants grown in sandy …

Northern Corn Leaf Blight, Tamra A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

Northern corn leaf blight (NCLB) is a disease of corn caused by a fungus, Exserohilum turcicum (sexual stage Setosphaeria turcica). Its development is favored by cool to moderate temperatures and high relative humidity. Historically, NCLB has been more common and severe in states in the eastern Corn Belt, but its incidence has increased in Nebraska and the western Corn Belt in recent years. The disease is distributed throughout most of the corn-growing areas of the United States. The disease also occurs sporadically throughout other humid corn-producing areas of the world. In Nebraska, the disease has been most serious in …

Identification Of Erwinia Rhapontici As The Causal Agent Of Crown And Shoot Rot And Pink Seed Of Pea In Nebraska, Anthony O. Adesemoye, Hsin-Ho Wei, Robert M. Harveson

Department of Plant Pathology: Faculty Publications

Over the last five years, the production of dry yellow peas (Pisum sativum L.) has been increasing in Nebraska and other areas of the Central High Plains, according to a USDA report (Jasa 2013). Dry pea is a short-season crop with a low water requirement, making it a good rotational crop for the high plains. We have noted bacterial pathogens, potentially a disease complex, that may negatively impact the production of pea in this region, and one of the emerging pathogens is Erwinia rhapontici. This pathogen is a gram-negative bacterium that has been reported from soil, seed, and …

Transcriptome Profiling Of Buffalograss Challenged With The Leaf Spot Pathogen Curvularia Inaequalis, Bimal S. Amaradasa, Keenan Amundsen

Department of Plant Pathology: Faculty Publications

Buffalograss (Bouteloua dactyloides) is a low maintenance U. S. native turfgrass species with exceptional drought, heat, and cold tolerance. Leaf spot caused by Curvularia inaequalis negatively impacts buffalograss visual quality. Two leaf spot susceptible and two resistant buffalograss lines were challenged with C. inaequalis. Samples were collected from treated and untreated leaves when susceptible lines showed symptoms. Transcriptome sequencing was done and differentially expressed genes were identified. Approximately 27 million raw sequencing reads were produced per sample. More than 86% of the sequencing reads mapped to an existing buffalograss reference transcriptome. De novo assembly of unmapped reads …

Morphological And Molecular Characterization Of Gracilacus Wuae N. Sp. (Nematoda: Criconematoidea) Associated With Cow Parsnip (Heracleum Maximum) In Ontario, Canada, Qing Yu, Weimin Ye, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

Gracilacus wuae n. sp. from soil associated with cow parsnip in Ontario, Canada is described and illustrated. Morphologically, females have a long stylet ranging from 80 to 93 mmlong, the lip region not offset from the body contour, without lateral lips but with large and flat submedian lobes, the mouth opening slit-like elongated laterally and surrounded by lateral flaps, the excretory pore is anterior to the knobs of the stylet; males without stylet and the pharynx degenerated. The fourth-stage juveniles lack a stylet, the pharynx degenerated, and can be differentiated into preadult females and males based on the position of …

Mechanisms, Applications, And Perspectives Of Antiviral Rna Silencing In Plants / Mecanismos, Aplicaciones Y Perspectivas Del Silenciamiento Génico De Virus En Plantas, Hernan Garcia-Ruiz, Mayra Teresa Garcia Ruiz, Sergio Manuel Gabriel Peralta, Cristina Betzabeth Miravel Gabriel, Kaoutar El-Mounadi

Department of Plant Pathology: Faculty Publications

Viral diseases of plants cause important economic losses due to reduction in crop quality and quantity to the point of threatening food security in some countries. Given the reduced availability of natural sources, genetic resistance to viruses has been successfully engineered for some plant-virus combinations. A sound understanding of the basic mechanisms governing plant-virus interactions, including antiviral RNA silencing, is the foundation to design better management strategies and biotechnological approaches to engineer and implement antiviral resistance in plants. In this review, we present current molecular models to explain antiviral RNA silencing and its application in basic plant research, biotechnology and …

Incidence Of Wheat Streak Mosaic Virus, Triticum Mosaic Virus, And Wheat Mosaic Virus In Wheat Curl Mites Recovered From Maturing Winter Wheat Spikes, E. Byamukama, S. Tatineni, G. Hein, J. Mcmechan, S. N. Wegulo

Department of Plant Pathology: Faculty Publications

Wheat curl mites (WCM; Aceria tosichella) transmit Wheat streak mosaic virus (WSMV), Triticum mosaic virus (TriMV), and Wheat mosaic virus (WMoV) to wheat (Triticum aestivum L.) in the Great Plains region of the United States. These viruses can be detected in single, double, or triple combinations in leaf samples. Information on incidence of viruses inWCM at the end of the growing season is scant. The availability of this information can enhance our knowledge of the epidemiology ofWCM-transmitted viruses. This research was conducted to determine the frequency of occurrence of WSMV, TriMV, and WMoV in WCM populations on fieldcollected maturing …

Developing The Framework For A Risk Map For Mite Vectored Viruses In Wheat Resulting From Pre-Harvest Hail Damage, Anthony L. Nguy-Robertson, Arthur Zygielbaum, Anthony J. Mcmechan, Gary L. Hein, Stephen N. Wegulo, Abby R. Stilwell, Travis M. Smith

Department of Plant Pathology: Faculty Publications

There is a strong economic incentive to reduce mite-vectored virus outbreaks. Most outbreaks in the central High Plains of the United States occur in the presence of volunteer wheat that emerges before harvest as a result of hail storms. This study provides a conceptual framework for developing a risk map for wheat diseases caused by mite-vectored viruses based on pre-harvest hail events. Traditional methods that use NDVI were found to be unsuitable due to low chlorophyll content in wheat at harvest. Site-level hyperspectral reflectance from mechanically hailed wheat showed increased canopy albedo. Therefore, any increase in NIR combined with large …

Sequence Variation In Two Genes Determines The Efficacy Of Transmission Of Citrus Tristeza Virus By The Brown Citrus Aphid, S. J. Harper, N. Killiny, Satyanarayana Tatineni, S. Gowda, S. J. Cowell, T. Shilts, W. O. Dawson

Department of Plant Pathology: Faculty Publications

Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in to the poorly transmissible T36-based infectious clone. We found that introducing p65 or p61 sequences from FS577 significantly increased transmission efficacy. Interestingly, replacement of both genes produced a greater increase than either gene alone, suggesting that CTV transmission requires the concerted action of co-evolved p65 and p61 proteins.

Field Damage Of Sorghum (Sorghum Bicolor) With Reduced Lignin Levels By Naturally Occurring Insect Pests And Pathogens, Patrick F. Dowd, Deanna L. Funnell-Harris, Scott E. Sattler

Department of Plant Pathology: Faculty Publications

Sorghum (Sorghum bicolor (L.) Moench) brown midrib (bmr) mutant lines have reduced levels of lignin, which is a potentially useful trait for bioenergy production, but the effects of this trait on insect and plant pathogen interactions are unknown under field conditions. Field-grown bmr6, bmr12, and wild-type (WT) plants were examined for insect and disease damage. In most cases, observed frequency, population, or leaf area damage caused by insects or pathogens on bmr6 or bmr12 plants were not greater than those observed on WT plants in the field or laboratory assays. European corn borers [Ostrinia nubilalis (Hübner)(Lepidoptera: …

What's New In Plant Pathology, Anthony O. Adesemoye, Loren Giesler, Robert M. Harveson, Tamra A. Jackson-Ziems, Kevin Korus, Stephen N. Wegulo

Department of Plant Pathology: Faculty Publications

Changes to the Disease Management Section of the 2016 Guide for Weed, Disease, and Insect Management in Nebraska

Biological control Products

Trivapro Fungicide

Priaxor D. Fungicide

Table 1. Foliar products for disease control that were updated in the 2016 Guide for Weed, Disease and Insect Management in Nebraska.

Table 2. Seed treatment products for disease control that were updated in the 2016 Guide for Weed, Disease and Insect Management in Nebraska.

Table 3. Seed treatment nematicide product that was updated in the 2016 Guide for Weed, Disease and Insect Management in Nebraska.

Fungicide Application Timing And Disease Control, Tamra A. Jackson-Ziems, Loren J. Giesler, Robert M. Harveson, Stephen N. Wegulo, Kevin Korus, Anthony O. Adesemoye

Department of Plant Pathology: Faculty Publications

Wet weather that persisted during much of the 2015 growing season proved to be very favorable for disease development in several crops across Nebraska. In particular, diseases caused by fungi were favored by repeated rainfall events and high relative humidity, fog, etc., that supported the production of more spores and continuous plant infection. On some susceptible varieties and hybrids, diseases developed early and quickly eventually reaching moderate to severe levels. Foliar fungicides were applied one or more times to some crops in an effort to mitigate losses due to disease. However, low crop prices and narrow profit margins made foliar …

Corn Disease Update, Tamra A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The 2015 corn crop was impacted by several corn diseases from the beginning to the end of the season and in all areas of Nebraska. The increased severity and incidence of some diseases was largely due to the extended periods of favorable weather conditions in varying parts of the state that supported the increase in some diseases. In particular, frequent rainfall and high relative humidity were especially favorable for several fungal diseases. Unfortunately, almost all diseases of corn (except rusts) are caused by pathogens that can successfully overwinter in Nebraska. For this reason, making detailed notes on disease development and …

Trivapro A + Trivapro B Application Timing And Disease Efficacy In Field Corn In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The objective of the trial was to evaluate foliar fungicides and application timings for crop response and foliar disease efficacy. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % OM and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to grey leaf spot (GLS)) was planted at approximately 34,000 seed/A on 26 May. Seven treatments were arranged in a randomized complete block design with six replications. …

Fungicide And Herbicide Effects On Grey Leaf Spot In Nebraska Field Corn, 2015, J. D. Harbour, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The objective of the trial was to compare foliar fungicides applied to three growth stages of corn for grey leaf spot (GLS) efficacy. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % OM and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to gray leaf spot (GLS)) was planted at approximately 34,000 seed/A on 26 May. Five treatments were arranged in a randomized complete block design …

A Foliar Fungicide Plus Herbicide Tank Mixture Evaluation In Field Corn In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The objective of the trial was to evaluate the tank mixture of Laudis herbicide plus Stratego YLD fungicide for corn response. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % OM and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to gray leaf spot (GLS)) was planted at approximately 34,000 seed/A on 26 May. Four treatments were arranged in a randomized complete block design with six …

Corn Disease Profiles: Diseases Favored By Dry Conditions, Tamra A. Jackson-Ziems, Anthony O. Adesemoye, Loren J. Giesler, Robert M. Harveson, Kevin A. Korus, Stephen N. Wegulo

Department of Plant Pathology: Faculty Publications

Extension Circular 1910 (EC1910)

Extreme weather events are predicted to become increasingly common and could bring periods of drought as well as intense rainfall events. Wet conditions are favorable for many plant pathogens and the development of diseases, but some diseases may also develop during or following dry weather. The timing during the season when dry conditions occur, as well as other factors, such as temperature, impact which diseases develop and when. The list summarizes some of the most common corn diseases that can develop during dry conditions: Seedling Root Rot Diseases, Nematodes, Common Smut, Rust Diseases Charcoal Rot, Stalk …

Corn Disease Profiles: Diseases Favored By Wet Conditions, Tamra A. Jackson-Ziems, Anthony O. Adesemoye, Loren J. Giesler, Robert M. Harveson, Kevin A. Korus, Stephen N. Wegulo

Department of Plant Pathology: Faculty Publications

Extension Circular 1909 (EC1909)

Extreme weather events are predicted to become increasingly common and could bring periods of more intense rainfall. Wet conditions are favorable for many plant pathogens and the development of diseases. Seasonal timing when these conditions occur, as well as other factors such as temperature, impact which diseases develop and when. Listed, described, and illustrated are some common corn diseases favored by wet conditions: Pythium Root Rot, Eyespot, Northern Corn Leaf Blight, Gray Leaf Spot, Physoderma Brown Spot, Rust Diseases (Puccinia spp.), Stalk and Crown Rot Diseases (such as those caused by Fusarium and Diplodia spp.), …

Cryptic Species: A Leitmotif Of Contemporary Mycology Has Challenges And Benefits For Plant Pathologists, Frank M. Dugan, Sydney Everhart

Department of Plant Pathology: Faculty Publications

Multiple traditional species names for plant pathogenic fungi have been supplemented with new names that delimit formerly cryptic species. In separate instances, isolates within a species are clearly differentiated by both phylogeny and distinctive pathogenic traits and are assigned sub-specific designations. These new species names and the sub-specific designations are both cases of cryptic species that are, in some instances, relevant and/or critical for plant disease management. Here we provide examples of such instances in which newly described taxa differ from the original (“parent”) in phenotypic traits of importance to plant disease management: host range, fungicide sensitivity, environmental niche, metabolite …

Foliar Fungicide Comparison For Southern Rust Management, Stay Green, Push Lodging, And Yield In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The objective of the trial was to compare foliar fungicides for southern rust (SR) efficacy. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % OM and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to gray leaf spot (GLS)) was planted at approximately 34,000 seed/A on 26 May. Eight treatments were arranged in a randomized complete block design with six replications. . . . Stalk lodging …

Association Of Neonectria Macrodidyma With Dry Root Rot Of Citrus In California, A. O. Adesemoye, J. S. Mayorquin, B. B. Peacock, K. Moreno, S. Hajeri, R. Yokomi, A. Eskalen

Department of Plant Pathology: Faculty Publications

During yearly surveys that started in 2010 to identify pathogens associated with dry root rot disease of citrus in California, samples with root rot symptoms were collected in Tulare County. Small pieces of tissue from root samples were plated onto potato dextrose agar amended with 0.01% tetracycline and incubated at 25°C. Pure cultures of fungal isolates were identified by morphology and sequence analysis of the Internal Transcribed Spacer and Beta Tubulin regions. Neonectria macrodidyma (Cylindrocarpon macrodidymum) was first recovered in 2011 and has subsequently been recovered multiple times from citrus samples. The pathogen appeared to be widely distributed …

Chromatin Immunoprecipitation (Chip) Assay For Detecting Direct And Indirect Protein -Dna Interactions In Magnaporthe Oryzae, Gang Li, Margarita Marroquin-Guzman, Richard A. Wilson

Department of Plant Pathology: Faculty Publications

Chromatin immunoprecipitation (ChIP) is a powerful technology for analyzing protein-DNA interactions in cells. Robust ChIP procedures have been established for investigating direct interactions between protein and DNA. However, detecting indirect protein-DNA interactions in vivo is challenging. Recently, we used ChIP to analyze an indirect protein-DNA interaction between a putative histone demethylase, MoJmjC, and the promoter of the superoxide dismutase 1-encoding gene MoSOD1 in the rice blast fungus Magnaporthe oryzae (M. oryzae) (Fernandez et al., 2014). We tagged MoJmjC with the 3x FLAG epitope (Fernandez et al., 2014), instead of the larger and more commonly used GFP epitope, to mitigate …

Transgenic Soybean Plants Expressing Asoybean Homolog Of Glycine-Rich Protein 7 (Grp7) And Exhibiting Improved Innate Immunity, James R. Alfano, Anna Joe, Thomas E. Clemente, Zhengqing Fu, Ming Guo, Byeong-Ryool Jeong, Thomas Elthon

Department of Plant Pathology: Faculty Publications

This disclosure provides for transgenic Soybean plants expressing a soybean homolog of glycine-rich protein 7 (GRP7) and exhibiting improved innate immunity and meth ods of making Such plants.

The Khmer Software Package: Enabling Efficient Nucleotide Sequence Analysis, Michael R. Crusoe, Hussien F. Alameldin, Sherine Awad, Elmar Boucher, Adam Caldwell, Reed Cartwright, Amanda Charbonneau, Bede Constantinides, Greg Edvenson, Scott Fay, Jacob Fenton, Thomas Fenzl, Jordan Fish, Leonor Garcia-Gutierrez, Phillip Garland, Jonathan Gluck, Iván González, Sarah Guermond, Jiarong Guo, Aditi Gupta, Joshua R. Herr, Adina Howe, Alex Hyer, Andreas Härpfer, Luiz Irber, Rhys Kidd, David Lin, Justin Lippi, Tamer Mansour, Pamela Mca'nulty, Eric Mcdonald, Jessica Mizzi, Kevin D. Murray, Joshua R. Nahum, Kaben Nanlohy, Alexander Johan Nederbragt, Humberto Ortiz-Zuazaga, Jeramia Ory, Jason Pell, Charles Pepe-Ranney, Zachary N. Russ, Camille Scott, Josiah Seaman, Scott Sievert 38, Jared Simpson, Connor T. Skennerton, James Spencer, Ramakrishnan Srinivasan, Daniel Standage, James A. Stapleton, Susan R. Steinman, Joe Stein, Benjamin Taylor, Will Trimble, Heather L. Wiencko, Michael Wright, Brian Wyss, Qingpeng Zhang, En Zyme, C. Titus Brown, Erich Schwarz

Department of Plant Pathology: Faculty Publications

The khmer package is a freely available software library for working efficiently with fixed length DNA words, or k-mers. khmer provides implementations of a probabilistic k-mer counting data structure, a compressible De Bruijn graph representation, De Bruijn graph partitioning, and digital normalization. khmer is implemented in C++ and Python, and is freely available under the BSD license at https://github.com/dib-lab/khmer.

A Unique 5' Translation Element Discovered In Triticum Mosaic Virus, Robyn Roberts, Jincan Zhang, Laura K. Mayberry, Satyanarayana Tatineni, Karen S. Browning, Aurelie M. Rakotondrafara

Department of Plant Pathology: Faculty Publications

Several plant viruses encode elements at the 5' end of their RNAs, which, unlike most cellular mRNAs, can initiate translation in the absence of a 5' m7GpppG cap. Here, we describe an exceptionally long (739-nucleotide [nt]) leader sequence in triticum mosaic virus (TriMV), a recently emerged wheat pathogen that belongs to the Potyviridae family of positive-strand RNA viruses. We demonstrate that the TriMV 5' leader drives strong cap-independent translation in both wheat germ extract and oat protoplasts through a novel, noncanonical translation mechanism. Translation preferentially initiates at the 13th start codon within the leader sequence independently of eIF4E but involves …

Gata-Dependent Glutaminolysis Drives Appressorium Formation In Magnaporthe Oryzae By Suppressing Tor Inhibition Of Camp/Pka Signaling, Margarita Marroquin-Guzman, Richard A. Wilson

Department of Plant Pathology: Faculty Publications

Fungal plant pathogens are persistent and global food security threats. To invade their hosts they often form highly specialized infection structures, known as appressoria. The cAMP/ PKA- and MAP kinase-signaling cascades have been functionally delineated as positive-acting pathways required for appressorium development. Negative-acting regulatory pathways that block appressorial development are not known. Here, we present the first detailed evidence that the conserved Target of Rapamycin (TOR) signaling pathway is a powerful inhibitor of appressorium formation by the rice blast fungus Magnaporthe oryzae. We determined TOR signaling was activated in an M. oryzae mutant strain lacking a functional copy of …