Plant Biology Commons^™

Effect Of Leaf Phosphorus And Potassium Concentration On Chlorophyll Meter Reading In Rice, Shaobing Peng, Arnel L. Sanico, Felipe V. Garcia, Rebecca C. Laza, Romeo M. Visperas, Josue P. Descalsota, Kenneth G. Cassman

Department of Agronomy and Horticulture: Faculty Publications

Chlorophyll meter (SPAD) is a convenient tool to estimate leaf nitrogen (N) concentration of rice plants. There is no information on the effects of leaf phosphorus (P) and potassium (K) concentration on SP AD readings and on the relationship between SPAD values and leaf N concentration in the literature. In 1996 dry season, cv IR72 was grown at the International Rice Research Institute (IRRI) and the Philippine Rice Research Institute (PhilRice) under various N, P and K fertilizer combinations. SPAD measurements were made on the topmost fully expanded leaves at mid-tillering and panicle initiation. The leaves were then detached, dried …

Loci Controlling Resistance To High Plains Virus And Wheat Streak Mosaic Virus In A B73 × Mo17 Population Of Maize, A. Marçon, S. M. Kaeppler, S. G. Jensen, L. Senior, C. Stuber

Department of Agronomy and Horticulture: Faculty Publications

High Plains disease has the potential to cause significant yield loss in susceptible corn (Zea mays L.) and wheat (Triticum aestivum L.) genotypes, especially in the central and western USA. The primary causal agent, High Plains virus (HPV), is vectored by wheat curl mite (WCM; Aceria tossicheila Keifer), which is also the vector of wheat streak mosaic virus (WSMV). In general, the two diseases occur together as a mixed infection in the field. The objective of this research was to characterize the inheritance of HPV and WSMV resistance using B73 (resistant to HPV and WSMV) × Mo17 (moderately …

Measurement Of Sorghum Stalk Strength Using The Missouri-Modified Electronic Rind Penetrometer, Jeffrey F. Pedersen, J. J. Toy

Department of Agronomy and Horticulture: Faculty Publications

Resistance to lodging is critical in sorghum [Sorghum bicolor (L.) Moench] grown for grain, yet adequate tools for plant breeders to assess this character at or before anthesis have not been developed. The objective of this study was to evaluate whether an electronic penetrometer previously shown to be an effective tool for measuring stalk strength in maize (Zea mays L.) could be usedd to differentiate among grain sorghum hybrids. Entries in three commercial grain sorghum hybrid trials were evaluated for rind penetrometer resistance (RPR) of the peduncle, the lower stalk anthesis and the lower stalk at maturity. Actual …

Growth Traits Among Sorghum Genotypes In Response To Aluminum, J. H. Bernal, R. B. Clark

Department of Agronomy and Horticulture: Faculty Publications

Aluminum (Al) has many detrimental effects on plant growth, and shoots and roots are normally affected differently. A study was conducted to determine differences among sorghum [Sorghum bicolor (L.) Moench] genotypes with broad genetic backgrounds for growth traits of plants grown at 0,200,400,600, and 800 uM Al in nutrient solutions (pH 4.0). Genotypes were categorized into "Al-sensitive", "intermediate Al-tolerant", "Al-tolerant", and SC 283 (an Al-tolerant standard). As Al increased, shoot and root dry matter (DM), net main axis root length (NMARL), and total root length (TRL) became lower than controls (0 Al). Aluminum toxicity and/or nutrient deficiency symptoms become …

Soil Temperature And Fumigation Effects On Plant Phosphorus Uptake And Related Microbial Properties, Dennis L. Mccallister, L. A. Jawson, M. D. Jawson

Department of Agronomy and Horticulture: Faculty Publications

Early season problems with growth of corn (Zea mays L.) under cool, wet conditions prompted a study of the effects of soil and environmental conditions on mineralization and plant uptake of phosphorus (P). Our objective was to determine the effect of soil test P, temperature, and soil fumigation on soil P availability and uptake during early corn growth. Corn was grown in growth chambers at temperatures of 14°C or 25°C. Soils were a high-P Hastings silty clay loam (fine, montmorillonitic, mesic Udic Argiustoll) and a low-P Sharpsburg clay loam (fine, montmorillonitic, mesic Typic Argiudoll). Plants grew for up to …

Molecular And Functional Characterization Of A Novel Low-Affinity Cation Transporter (Lct1) In Higher Plants, Daniel P. Schachtman, Raman Kumar, Julian I. Schroeder, Ellen L. Marsh

Department of Agronomy and Horticulture: Faculty Publications

The transport of cations across membranes in higher plants plays an essential role in many physiological processes including mineral nutrition, cell expansion, and the transduction of environmental signals. In higher plants the coordinated expression of transport mechanisms is essential for specialized cellular processes and for adaptation to variable environmental conditions. To understand the molecular basis of cation transport in plant roots, a Triticum aestivum cDNA library was used to complement a yeast mutant deficient in potassium (K⁺) uptake. Two genes were cloned that complemented the mutant: HKT1 and a novel cDNA described in this report encoding a cation …

Notes: Forage Yield, Quality, And Fertility Of Sorghum X Sudan Grass Hybrids In A1 And A3 Cytoplasm, Jeffrey F. Pedersen, J. J. Toy

Department of Agronomy and Horticulture: Faculty Publications

Currently, no information is available comparing the agronomic performance of A1 and A3 cytoplasms in sorghum x sudangrass [Sorghumbi color (L.) Moench] hybrids. The objectives of this study were to compare the effects of A1 and A3 cytoplasm on the maturity, fertility, height, forage yield, and forage quality of sorghum × sudangrass hybrids. In 1989, pollen from a bulk of eight sudangrass populations was used to pollinate four sorghum lines that had been male-sterilized in both A1 and A3 cytoplasm. Hybrids were grown at the Univ. of Nebraska Field Laboratory Ithaca, NE, in 1990 and 1991, in a split-plot …

Media Effects On Phenotype Of Callus Cultures Initiated From Photoperiod-Insensitive, Elite Inbred Sorghum Lines, H. F. Kaeppler, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Sorghum (Sorghum bicolor L. Moench) is a crop of worldwide agronomic importance. Routine production of high quality (friable, embryogenic, fast growing) callus cultures is fundamental to tissue culture based genetic study and improvement of sorghum. Genotype by culture medium interactions for sorghum callus growth and morphology have been previously reported. The objective of this study was to identify tissue culture media that would support high quality callus growth across photoperiod insensitive, relatively elite genotypes. Explants from immature inflorescences of 11 sorghum genotypes were cultured on 6 tissue culture media of differing composition. After 3 and 5 months in culture, calli …

Germplasm And Cultivar Development, M. D. Casler, Jeffrey F. Pedersen, G. C. Eizenga, S. D. Stratton

Department of Agronomy and Horticulture: Faculty Publications

Cool-season forage grasses have evolved, and continue to evolve, in natural ecosystems subject to environmental factors both in the presence and absence of human influences. The literature often lacks facts describing the evolution and domestication of forage grasses. Furthermore, the literature on this subject mainly deals with evolution of species in the broad scope, i.e., on a scale of hundreds of thousands or millions or years. Thus, some of our conclusions are necessarily speculative and are highly subject to the nature of the research that has been reported. We describe the forces of selection that act upon cool-season forage grasses …

Annual Forages: New Approaches For C-4 Forages, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

The current agricultural paradigm in the U.S. is heavily biased towards the production and marketing of crops as commodities. This paradigm is kept in place by grain handling and marketing infrastructure, as well as government farm programs, designed for crops as commodities. Sorghum (Sorghum bicolor L. Moench) and maize (Zea mays L.) grown for grain certainly fit into this current paradigm.

A new approach to agricultural production and marketing, identity preserved products, is gaining in importance. This is made possible through new technologies and markets demanding products designed specifically for their needs. Examples of investment in identity preserved …

Breeding Sorghum And Pearl Millet For Forage And Fuel, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Sorghum [Sorghum bicolor (L.) Moench] and pearl millet [Pennisetum glaucum (L.) R. Br.] are unique species in their ability to be used in many forage llivestock system roles. Such flexibility has made prioritizing breeding objectives difficult and has even contributed to contradictory opinions on appropriate forage breeding objectives. Few breeding projects identified in the USDA-ARS, USDA-CREES, or at ICRISAT had forage sorghum or forage pearl millet as their sole research assignment. In the United States, it can be argued that breeding resources committed to forage sorghum improvement are probably declining. A new forage sorghum and forage pearl millet …

Application Of The Single Kernel Wheat Characterization Technology To Sorghum Grain, Jeffrey F. Pedersen, C. R. Martin, F. C. Felker, J. L. Steele

Department of Agronomy and Horticulture: Faculty Publications

A single kernel wheat characterization system (SKWCS) was recently developed by the USDA, ARS Grain Marketing Research Laboratory and is currently being marketed by Perten Instruments North America, Inc. This device has been shown to accurately measure individual seed hardness, moisture, and size of wheat. The objective of this study was to determine if the SKWCS technology could be applied to the measurement of sorghum grain. Grains from 64 sorghum plots grown at Mead, NE in 1992 were characterized using a prototype SKWCS at the USDA, ARS Grain Marketing Research Laboratory. Problems encountered were primarily associated with the single kernel …

In Vitro Starch Disappearance Procedure Modifications, C. J. Richards, Jeffrey F. Pedersen, R. A. Britton, R. A. Stock, C. R. Krehbiel

Department of Agronomy and Horticulture: Faculty Publications

Four in vitro experiments evaluated the effects of ruminal fluid inoculum:artificial saliva ratios,

grinder type, grind size, and diet of ruminal fluid donor on in vitro starch disappearance. Experiment 1 examined rates of starch disappearance and coefficients of determination obtained by linear regression of starch disappearance using five grain sorghum (Sorghum bicolur (L.) Moench) lines, a corn (Zeu muys L.) control, and a wheat (Triticum aestivum L.) control. Grains were incubated for 4, 8, 12, 16, and 20 h with inoculum varying in proportion of ruminal fluid and artificial saliva ( l:l, 1:2, 1:3, and 1:4). In …

An Automated Plot Harvest System For Use With A Commercial Forage Harvester, Jeffrey F. Pedersen, Kenneth J. Moore

Department of Agronomy and Horticulture: Faculty Publications

This automated harvest system is based on minimal modification of currently available commercial forage harvesters. Using 6.8 m plots separated by 0.75 m alleys, a single operator can harvest, finely chop, and collect wet plot weights of forage sorghum [Sorghum bicolor (L.) Moench] or pearl millet [Pennisetum glaucum (L.) R. Hr.] in <1 min per plot. Harvested material is deposited in a pile in the center of each plot. Subsamples for moisture and quality analysis can easily and safely be obtained from the pile.

Brown· Midrib Sorghum Silage For Midlactation Dairy Cows, R. J. Grant, S. G. Haddad, K. J. Moore, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Brown midrib sorghum silage was compared with alfalfa, corn, and normal sorghum silages for its effect on performance, ruminal metabolism, and digestive kinetics of Holstein dairy cows in midlactation. Twelve cows averaging 90 ± 5 DIM were assigned to one of four diets in replicated 4 X 4 Latin squares with 4-wk periods. Additionally, 3 ruminally fistulated cows (95 ± 20 DIM) were assigned to the same diets in a 3x 4 Youden square for measurement of ruminal characteristics. Diets were fed as isonitrogenous TMR that contained 65% silage (OM basis). The DMl was greater for the corn and brown …

Cdna Sequence For The Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase Complex Protein A Protein That Accumulates In Soybean Leaves In Response To Fruit Removal, Paul E. Staswick, Steven J. Crafts-Brandner

Department of Agronomy and Horticulture: Faculty Publications

Soybean (Glycine max L.) transiently accumulates two abundant vegetative storage proteins, VSP A and VSP B, in vacuoles of above-ground vegetative tissues (Wittenbach, 1983; Staswick, 1990). As older leaves and stems become a source of exported metabolites for developing sinks, the VSPs are preferentially degraded and the resulting metabolites are presumably mobilized in the xylem and phloem stream. A striking feature of these proteins is that VSP gene expression is dramatically increased in mature leaves following the remova1 of reproductive sinks (seed pods) and the amount of these proteins increases accordingly (for review, see Staswick, 1994). Other changes …

Models To Predict Yield Loss Due To Weeds In Rice Ecosystems, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Yield loss models have been developed and evaluated for irrigated rice ecosystems with respect to effects of Echinochloa crusgalli. Simple regression models that give the relation between yield loss and weed density or relative leaf area are discussed with respect to their practical value in weed management in rice. More complex eco-physiological process models have been developed and used for rice ecosystems as well. Such models can be useful as a tool in research, whereas more simple approaches are needed for practical application in weed management.

Purification Of The Major Soybean Leaf Acid Phosphatase That 1s Increased By Seed-Pod Removal, Paul E. Staswick, Chuck Papa, Jing-Feng Huang, Yoon Rhee

Department of Agronomy and Horticulture: Faculty Publications

Fruit removal for 5 weeks after flowering increased acid phosphatase activity 10-fold in soybean (Glycine max 1. Merr. vai Hobbit) leaves compared with normal seed-pod-bearing plants. The major acid phosphatase activity in leaves was purified over 2700-fold, yielding a single polypeptide of 51 kD with a specific activity of 1353 units/mg protein using p-nitrophenylphosphate as the substrate. lsoelectric focusing demonstrated that the purified protein co-migrated with a majority of the activity that increased in leaves following seed-pod removal. lmmunoblot analysis demonstrated that at least part of the increased activity was dueto an increased abundance of the phosphatase protein. In situ …

Breeding Systems For Cross-Pollinated Perennial Grasses, K. P. Vogel, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

I. Introduction

II. Reproductive and Breeding Characteristics

III. Breeding Systems

A. Ecotype Selection

B. Recurrent, RestricteclPhenotypic Selection

C. Half-sib Progeny Test

D. Between and Within Family Selection

E. Recurrent Multistep Family Selection

IV. Gains From Selection

A. Time Interval per Cycle

B. Potential Gain per Cycle .

C. Potential Inbreeding per Cycle

V. Polycrossing

VI. Hybrid Cultivars

VII. Conclusions

Literature Cited

Developmental Morphology Of Tropical Sorghum And Sorghum X Sudangrass, R. B. Mitchell, K. J. Moore, Jeffrey F. Pedersen, T. A. Peterson, L. E. Moser, D. D. Redfearn

Department of Agronomy and Horticulture: Faculty Publications

Tropical sorghum and sorghum x sudangrass are known to be efficient scavengers of soil nitrogen and are important forage crops in the U.S. Nitrate contamination of groundwater due to disposal of organic wastes and excessive fertilization is a major concern in the Central Great Plains. A common disposal method of municipal wastes is application to fallow cropland. Quantification of the developmental morphology of tropical sorghum and sorghum x sudangrass is important to developing management strategies that allow multiple applications of organic wastes to optimize nitrogen removal and dry matter production. The objective of this study was to quantify the developmental …

Genetic Manipulation Of Tall Fescue, Jeffrey F. Pedersen, D. A. Sleper

Department of Agronomy and Horticulture: Faculty Publications

Genetic manipulation of tall fescue (Festuca arnndinacea Schreb.) has not been altered by the discovery of the Acremonium coenophialum (Morgan-Jones and Gams) / grass interaction. However, tall fescue breeding programs have been affected greatly. The basic methods for genetically manipulating the grass have remained static. Tall fescue is an obligate out-crossing species, and most improvements are, therefore, captured in the form of an improved population developed through some form of mass or recurrent selection. What has changed is the breeder's ability to recognize genetic differences in the grass because of the confounding effect of A. coenophialum on plant phenotype. It …

Comparison Of Sorghum And Indiangrass Chloroplast Genomes Using Rflps, Jeffrey F. Pedersen, R. D. Lee, D. J. Lee, K. P. Vogel

Department of Agronomy and Horticulture: Faculty Publications

Sorghum (Sorghum bicolor (L.) Moench) and indiangrass (Sorghastrum nutans (L.) Nash) appear closely related based on morphological and chemotaxonomic characters. Each species could potentially provide desirable traits to the other. However, traditional breeding techniques have been unsuccessful in hybridizing these two species. The objective of this study was to determine the relatedness of sorghum and indiangrass chloroplast DNA using RFLPs. Eleven sorghum lines in several cytoplasms, two indiangrass popu1ations, and a corn line were studied using 60 proberestriction enzyme combinations. Principal component analysis of the results showed sorghum to be as closely related to corn as to indiangrass, with no …

Jasmonate, Genes, And Fragrant Signals, Paul E. Staswick

Department of Agronomy and Horticulture: Faculty Publications

Although first recognized for its growth-inhibiting activity about 20 years ago, JA² and its fragrant methyl ester MeJA (referred to here collectively as jasmonate) are receiving renewed interest as potentially important signaling molecules in plants. This is because jasmonate markedly increases the expression of specific plant genes, some of which are wound responsive. Similarities with fatty acid-derived stress signaling molecules in animals (1) make jasmonate of general biological interest.

Nitrogen And Methyl Jasmonate Induction Of Soybean Vegetative Storage Protein Genes, Paul E. Staswick, Jing-Feng Huang, Yoon Rhee

Department of Agronomy and Horticulture: Faculty Publications

Vegetative storage protein (VSP) and VSP mRNA levels in soybean (Glycine max) leaves correlated with the amount of NH₄NO₃ provided to nonnodulated plants. The mRNA level declined as leaves matured, but high levels of N delayed the decline. This is consistent with the proposed role for VSP in the temporary storage of N. Wounding, petiole girdling, and treatment with methyijasmonate (MeJA) increased VSP mRNA in leaves 24 hours after treatment. The magnitude of the response depended on leaf age and N availability. N deficiency essentially eliminated the response to wounding and petiole girdling. MeJA was …

Inheritance Of Acid-Soil Tolerance In Sorghum (Sorghum Bicolor) Grown On An Ultisol, C. I. Flores, L. M. Gourley, Jeffrey F. Pedersen, R. B. Clark

Department of Agronomy and Horticulture: Faculty Publications

Inheritance of acid-soil tolerance (generally considered AI-toxicity tolerance) i.n sorghum [Sorghum bicolor (L.) Moench] is not clear. Forty F₁ sorghum hybrids and their 14 parents were grown two seasons in the field at relatively high (67 and 71%) and low (43 and 42%) Al saturations on an acid Ultisol in Colombia, South America to evaluate the effects of acid soil on agronomic component traits and to better understand inheritance of acid-soil tolerance of sorghum. For plants grown at the high Al saturation levels, hybrids from acid-soil tolerant [AS-T] x acid soil-sensitive [AS-S] crosses were as tolerant as hybrids from …

Fertility And Forage Yield Of Sorghum X Sudangrass Hybrids In A1 And A3 Cytoplasm, J. J. Toy, Jeffrey F. Pedersen, K. J. Moore

Department of Agronomy and Horticulture: Faculty Publications

Most sorghum x sudangrass hybrids are currently produced in Al male-sterile cytoplasm. Availability of alternative cytoplasmic sterility systems allows production of sorghum x sudangrass hybrids that may have superior forage characteristics. A study was conducted to compare the agronomic performance of Al and A3 sorghum x sudangrass hybrids. A bulk of eight sudangrass populations was used to pollinate four grain sorghum inbreds normally used as females that had been sterilized in both Al and A3 cytoplasm, and two inbreds normally used as males that had been sterilized in A3 cytoplasm. The hybrids were evaluated in 1990. Results indicate that male-sterile …

Notes: Interpretive Analysis For Forage Yield Trial Data, Jeffrey F. Pedersen, K. J. Moore, Edzard Van Santen

Department of Agronomy and Horticulture: Faculty Publications

Forage cultivar evaluation is often done in small plots with multiple harvests throughout the growing season. Data is often summarized by presenting a yearly total yield for each cultivar in addition to the mean for each harvest date. Data summarization often becomes burdensome and difficult to interpret. Regressing yield against a growth index associated with harvest dates can be utilized to describe forage performance in a concise and easily interpreted format. Subsets of data from tall fescue (Festuca amndinacea Schreb.) yield trials conducted in Alabama and Kentucky were used to demonstrate the technique. The analysis involves regressing yield of a …

Fescues With Large Roots Are Drought Tolerant, H. A. Torbert, J. H. Edwards, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Consideration of root/soil interactions is essential in, adapting tall fescue (Festuca arundinacea Schreb.) to soil and climatic conditions of the Coastal Plain region of the southeast. Sandy soils of the region are very susceptible to the formation of hardpans. These compacted layers often restrict plant root development to the plow layer (0 to 6 in.) and prevent plant roots from reaching available moisture and nutrients in the subsoil horizons. Plants grown under these conditions are shallow rooted and will be subjected to water stress up to 50% of the time during the growing season. Although implements are available to penetrate …

A Review Of The Agronomic Characteristics Of Endophyte-Free And Endophyte-Infected Tall Fescue, Jeffrey F. Pedersen, G. D. Lacefield, D. M. Ball

Department of Agronomy and Horticulture: Faculty Publications

Agronomic differences between endophyte- (Acremonium coenophialum Morgan Jones and Gams) free and endophyte-infected tall fescue (Festuca arundinacea Schreb.) exist, and should be considered when implementing a management strategy. Although dry matter yield of tall fescue does not generally appear to be influenced by endophyte infection status, endophyte infection has been shown to improve seedling performance and survival, is associated with insect and nematode resistance, drought resistance, improved nitrogen assimilation, and higher seed set. Considering all biologically valuable characters of the endophyte-tall fescue relationship, survival of endophyte-infected tall fescue is probably better than that of endophyte-free tall fescue, especially in drought- …

Evaluation Of Seeding Rates Of Au Triumph And Endophyte-Infected Kentucky 31 Tall Fescue, Jeffrey F. Pedersen, D. M. Ball

Department of Agronomy and Horticulture: Faculty Publications

Increased difficulty in stand establishment of tall fescue (Festuca arundinacea Schreb.) has been reported by producers since the introduction of endophyte (Acremonium coenophialum Morgan Jones and Gams)-free tall fescue seed into the market place. This has encouraged the use of higher seeding rates for endophyte-free tall fescue without scientific basis. The objective of this study was to determine whether ‘AU Triumph’, and endophyte-free cultivar, and endophyte-infected ‘Kentucky 31’ differ in minimum seeding rates for successful establishment. Experiments were established at two locations in each of two years, using a randomized complete block with four replications. Soils at the two locations …