Plant Biology Commons^™

Center For Grassland Studies, March 2022

Center for Grassland Studies: Newsletters

Contents

New Course: Research Experiences in Grasslands by Dan Uden

Center for Grassland Studies Policy Advisory Committee

Director’s Column by Jerry Volesky (Interim Director)

Educational Trunk to Focus on Grasslands by Cheryl Dunn

Form a Team and Grab Your Clubs by Scott Holly

Collaborative Adaptive Management for the Barta Brothers Ranch by Craig Allen

Nebraska Range Short Course, June 20-23

Registration Opens for 2022 Nebraska Ranch Practicum by Troy Walz

Nebraska Sandhills Rates as World’s Most Intact Prairie by Scott Schrage

Nebraska Youth Range Camp, June 6-9, Nebraska College of Technical Agriculture, Curtis, Nebraska

Impacts Of Temperature And Carbon Dioxide On Boro Rice Production, Sabiha Ferdous

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

There are different anticipated impacts of climate change (CC) on agriculture all over the world. Bangladesh as a developing country has potential vulnerability and negative impacts on CC. This change in meteorological parameters creates negative impacts on agricultural production which is a threat to food security and economic development. The country has recently achieved food security in rice which is mainly due to increased yields and area coverage of irrigated dry season (Boro) rice over the last decades. It is necessary to predict the future Boro rice production in accordance with CC as well as take supplementary actions to ensure …

Ecology And Diversity Of Culturable Fungal Species Associated With Soybean Seedling Diseases In The Midwestern United States, Mirian F. Pimentel, Ali Y. Srour, Amanda J. Warner, Jason P. Bond, Carl A. Bradley, John Rupe, Martin I. Chilvers, J. Alejandro Rojas, Janette L. Jacobs, Christopher R. Little, Alison E. Robertson, Loren J. Giesler, Dean Malvick, Kiersten Wise, Albert Tenuta, Ahmad M. Fakhoury

Department of Plant Pathology: Faculty Publications

Aims: To isolate and characterize fungi associated with diseased soybean seedlings in Midwestern soybean production fields and to determine the influence of environmental and edaphic factors on their incidence.

Methods and Results: Seedlings were collected from fields with seedling disease history in 2012 and 2013 for fungal isolation. Environmental and edaphic data associated with each field was collected. 3036 fungal isolates were obtained and assigned to 76 species. The most abundant genera recovered were Fusarium (73%) and Trichoderma (11.2%). Other genera included Mortierella, Clonostachys, Rhizoctonia, Alternaria, Mucor, Phoma, Macrophomina and Phomopsis. …

Improving Taxonomic Delimitation Of Fungal Species In The Age Of Genomics And Phenomics, Asjley Stengel, Kimberly M. Stanke, Amanda C. Quattrone, Joshua Herr

Department of Plant Pathology: Faculty Publications

Species concepts have long provided a source of debate among biologists. These lively debates have been important for reaching consensus on how to communicate across scientific disciplines and for advancing innovative strategies to study evolution, population biology, ecology, natural history, and disease epidemiology. Species concepts are also important for evaluating variability and diversity among communities, understanding biogeographical distributions, and identifying causal agents of disease across animal and plant hosts. While there have been many attempts to address the concept of species in the fungi, there are several concepts that have made taxonomic delimitation especially challenging. In this review we discuss …

The Astounding World Of Glycans From Giant Viruses, Anna Notaro, Chantal Abergel, Rosa Lanzetta, Todd L. Lowary, Antonio Molinaro, Michela Tonetti, James L. Van Etten, Cristina De Castro

Department of Plant Pathology: Faculty Publications

Viruses are a heterogeneous ensemble of entities, all sharing the need for a suitable host to replicate. They are extremely diverse, varying in morphology, size, nature, and complexity of their genomic content. Typically, viruses use host-encoded glycosyltransferases and glycosidases to add and remove sugar residues from their glycoproteins. Thus, the structure of the glycans on the viral proteins have, to date, typically been considered to mimick those of the host. However, the more recently discovered large and giant viruses differ from this paradigm. At least some of these viruses code for an (almost) autonomous glycosylation pathway. These viral genes include …

Hybrid Seed Set In Relation With Male Floral Traits, Estimation Of Heterosis And Combining Abilities For Yield And Its Components In Wheat (Triticum Aestivum L.), Samira El Hanafi, Souad Cherkaoui, Zakaria Kehel, Miguel Sanchez-Garcia, Jean-Benoit Sarazin, Stephen Baenziger, Wuletaw Tadesse

Department of Agronomy and Horticulture: Faculty Publications

Breeding hybrids with maximum heterosis requires efficient cross-pollination and an improved male sterility system. Renewed efforts have been made to dissect the phenotypic variation and genetic basis of hybrid floral traits, although the potential of tailoring the appropriate flower design on seed setting is less known. To this end, elite wheat genotypes were crossed using a chemical hybridizing agent at different doses. A total of 23 hybrids were developed from a partial diallel design; and planted in an alpha lattice design with their parents at two locations in Morocco, for two years, to evaluate for yield components, heterosis and combining …

Post-Termination Effects Of Cover Crop Monocultures And Mixtures On Soil Inorganic Nitrogen And Microbial Communities On Two Organic Farms In Illinois, Eleanor E. Lucadamo, Ashley A. Holmes, Samuel E. Wortman, Anthony C. Yannarell

Department of Agronomy and Horticulture: Faculty Publications

Cover crops can continue to affect agricultural systems even after they have been terminated by influencing nitrogen dynamics and by altering soil microbial communities. These post-termination effects can influence soil fertility, weed pressure, and the dynamics of potential plant pathogens in the narrow window of time between cover crop termination and cash crop emergence. We evaluated the post-termination effects of 12 different spring-sown cover crop mixtures and monocultures on soil nitrogen and microbial communities on two different organic farms in Central Illinois (on Lawson silt loam soil) and Northern Illinois (on Virgil silt loam soil). In comparison to control plots …

Post-Termination Effects Of Cover Crop Monocultures And Mixtures On Soil Inorganic Nitrogen And Microbial Communities On Two Organic Farms In Illinois, Eleanor E. Lucadamo, Ashley A. Holmes, Samuel E. Wortman, Anthony C. Yannarell

Department of Agronomy and Horticulture: Faculty Publications

Cover crops can continue to affect agricultural systems even after they have been terminated by influencing nitrogen dynamics and by altering soil microbial communities. These post-termination effects can influence soil fertility, weed pressure, and the dynamics of potential plant pathogens in the narrow window of time between cover crop termination and cash crop emergence. We evaluated the post-termination effects of 12 different spring-sown cover crop mixtures and monocultures on soil nitrogen and microbial communities on two different organic farms in Central Illinois (on Lawson silt loam soil) and Northern Illinois (on Virgil silt loam soil). In comparison to control plots …

Practice Cost And Size Differences In Invasive Plant Management Strategies: An Empirical Analysis Of Us Great Plains States, Mazbahul G. Ahamad

Department of Agronomy and Horticulture: Faculty Publications

Grassland conservation of the Conservation Stewardship Program in the United States (US) is one of the largest cost-sharing initiatives for protecting grazing land from invasive and woody plants. The practice cost and unit size of various invasive and woody plant management strategies, such as mulching, brush management, and pre- scribed burning, are different from state to state. We aimed to compare and examine the association between practice cost ($/acre[ac]) and standard unit size of practice (acre) of mulching, brush management, and pre- scribed burning strategies in nine US Great Plains states, including Kansas, Montana, Nebraska, New Mexico, North Dakota, Oklahoma, …

Implementation Of Epigenetic Variation In Sorghum Selection And Implications For Crop Resilience Breeding, Dikungwa Ketumile, Xiaodong Yang, Robersy Sanchez, Hardik Kundariya, John F. Rajewski, Ismail M. Dweikat, Sally A. Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Crop resilience and yield stability are complex traits essential for food security. Sorghum bicolor is an important grain crop that shows promise for its natural resilience to drought and potential for marginal land production. We have developed sorghum lines in the Tx430 genetic background suppressed for MSH1 expression as a means of inducing de novo epigenetic variation, and have used these materials to evaluate changes in plant growth vigor. Plant crossing and selection in two distinct environments revealed features of phenotypic plasticity derived from MSH1 manipulation. Introduction of an epigenetic variation to an isogenic sorghum population, in the absence of …

Assessment Of Bacterial Inoculant Delivery Methods For Cereal Crops, Yen Ning Chai, Stephanie Futrell, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Despite growing evidence that plant growth-promoting bacteria can be used to improve crop vigor, a comparison of the different methods of delivery to determine which is optimal has not been published. An optimal inoculation method ensures that the inoculant colonizes the host plant so that its potential for plant growth-promotion is fully evaluated. The objective of this study was to compare the efficacy of three seed coating methods, seedling priming, and soil drench for delivering three bacterial inoculants to the sorghum rhizosphere and root endosphere. The methods were compared across multiple time points under axenic conditions and colonization efficiency was …

Seed-Shattering Phenology At Soybean Harvest Of Economically Important Weeds In Multiple Regions Of The United States. Part 3: Drivers Of Seed Shatter, Lauren M. Schwartz-Lazaro, Lovreet S. Shergill, Jeffrey A. Evans, Muthukumar V. Bagavathiannan, Shawn C. Beam, Mandy D. Bish, Jason A. Bond, Kevin W. Bradley, William S. Curran, Adam S. Davis, Wesley J. Everman, Michael L. Flessner, Steven C. Haring, Nicholas R. Jordan, Nicholas E. Korres, John L. Lindquist, Jason K. Norsworthy, Tameka L. Sanders, Larry E. Steckel, Mark J. Vangessel, Blake Young, Steven B. Mirsky

Department of Agronomy and Horticulture: Faculty Publications

Seed retention, and ultimately seed shatter, are extremely important for the efficacy of harvest weed seed control (HWSC) and are likely influenced by various agroecological and environmental factors. Field studies investigated seed-shattering phenology of 22 weed species across three soybean [Glycine max (L.) Merr.]-producing regions in the United States. We further evaluated the potential drivers of seed shatter in terms of weather conditions, growing degree days, and plant biomass. Based on the results, weather conditions had no consistent impact on weed seed shatter. However, there was a positive correlation between individual weed plant biomass and delayed weed seed-shattering rates during …

Corn Nitrogen Nutrition Index Prediction Improved By Integrating Genetic, Environmental, And Management Factors With Active Canopy Sensing Using Machine Learning, Dan Li, Yuxin Miao, Curtis J. Ransom, Gregory Mac Bean, Newell R. Kitchen, Fabián G. Fernández, John E. Sawyer, James J. Camberato, Paul R. Carter, Richard B. Ferguson, David W. Franzen, Carrie A. M. Laboski, Emerson D. Nafziger, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Accurate nitrogen (N) diagnosis early in the growing season across diverse soil, weather, and management conditions is challenging. Strategies using multi-source data are hypothesized to perform significantly better than approaches using crop sensing information alone. The objective of this study was to evaluate, across diverse environments, the potential for integrating genetic (e.g., comparative relative maturity and growing degree units to key developmental growth stages), environmental (e.g., soil and weather), and management (e.g., seeding rate, irrigation, previous crop, and preplant N rate) information with active canopy sensor data for improved corn N nutrition index (NNI) prediction using machine learning methods. Thirteen …

Tracking Spatial Regimes In Animal Communities: Implications For Resilience-Based Management, Caleb P. Roberts, Daniel R. Uden, Craig R. Allen, David G. Angeler, Larkin A. Powell, Brady W. Allred, Matthew O. Jones, Jeremy D. Maestas, Dirac L. Twidwell Jr

Department of Agronomy and Horticulture: Faculty Publications

Spatial regimes (the spatial extents of ecological states) exhibit strong spatiotemporal order as they expand or contract in response to retreating or encroaching adjacent spatial regimes (e.g., woody plant invasion of grasslands) and human management (e.g., fire treatments). New methods enable tracking spatial regime boundaries via vegetation landcover data, and this approach is being used for strategic management across biomes. A clear advancement would be incorporating animal community data to track spatial regime boundaries alongside vegetation data. In a 41,170-hectare grassland experiencing woody plant encroachment, we test the utility of using animal community data to track spatial regimes via two …

Water Use Characteristics Of Weeds: A Global Review, Best Practices, And Future Directions, Mandeep Singh, Meetpal Singh Kukal, Suat Irmak, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Weeds usually penalize crop yields by competing for resources, such as water, light, nutrients, and space. Most of the studies on the crop-weed competition domain are limited to assessing crop-yield losses due to weed pressure and other crop-weed interactions, overlooking the significant uptake of soil-water by weeds that exacerbates global water constraints and threatens the productivity and profitability. The objective of this review was to synthesize globally available quantitative data on weed water use (WU) sourced from 23 peer-reviewed publications (filtered from 233 publications via a multi-step protocol of inclusion criteria) with experimental investigations across space (3 continents), time (1927–2018), …

Management-Intensive Grazing Impacts On Total Escherichia Coli, E. Coli O157: H7, And Antibiotic Resistance Genes In A Riparian Stream, Laura M. Rubeck, James E. Wells, Kathy Hanford, Lisa M. Durso, Walter H. Schacht, Elaine D. Berry

Department of Agronomy and Horticulture: Faculty Publications

The impacts of management-intensive grazing (MIG) of cattle on concentrations of total Escherichia coli, total suspended solids (TSS), and nitrate-nitrite nitrogen (NO₃+NO₂-N), and occurrence of E. coli O157:H7 and selected antibiotic resistance genes (ARGs) in stream water and/or sediments were evaluated. Cattle were grazed for twoweek periods in May in each of three years. Overall, grazing increased total E. coli in downstream water by 0.89 log10 MPN/100 mL (p < 0.0001), and downstream total E. coli concentrations were higher than upstream over all sampling intervals. Downstream TSS levels also increased (p ≤ 0.0294) during grazing. In contrast, there was …

The Last Continuous Grasslands On Earth: Identification And Conservation Importance, Rheinhardt Scholtz, Dirac L. Twidwell Jr

Department of Agronomy and Horticulture: Faculty Publications

Grasslands are the most threatened and least protected biome. Yet, no study has been conducted to identify the last remaining continuous grasslands on Earth. Here, we used World Wildlife Fund (WWF) and International Union for Conservation of Nature (IUCN) classifications to measure the degree of intactness remaining for the world's grassland ecoregions. This analysis revealed three findings of critical conservation importance. First, only a few large, intact grasslands remain. Second, every continent with a grassland ecoregion considered in this study contains at least one relatively intact grassland ecoregion. Third, the largest remaining continuous grasslands identified in this analysis have persisted …

Fddm1 And Fddm2, Two Sgs3-Like Proteins, Function As A Complex To Affect Dna Methylation In Arabidopsis, Shengjun Li, Weilong Yang, Yunfeng Liu, Guangyong Li, Xiang Liu, Yaling Liu, James R. Alfano, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

DNA methylation is an important epigenetic modification required for the specific regulation of gene expression and the maintenance of genome stability in plants and animals. However, the mechanism of DNA demethylation remains largely unknown. Here, we show that two SGS3-like proteins, FACTOR OF DNA DEMETHYLATION 1 (FDDM1) and FDDM2, negatively affect the DNA methylation levels at ROS1-dependend DNA loci in Arabidopsis. FDDM1 binds dsRNAs with 50 overhangs through its XS (rice gene X and SGS3) domain and forms a heterodimer with FDDM2 through its XH (rice gene X Homology) domain. A lack of FDDM1 or FDDM2 increased DNA methylation levels …

Qteller: A Tool For Comparative Multi-Genomic Gene Expression Analysis, Margaret R. Woodhouse, Shatabdi Sen, David Schott, John L. Portwood Ii, Michael Freeling, Justin W. Walley, Carson M. Andorf, James Schnable

Center for Plant Science Innovation: Faculty Publications

Motivation: Over the last decade, RNA-Seq whole-genome sequencing has become a widely used method for measuring and understanding transcriptome-level changes in gene expression. Since RNA-Seq is relatively inexpensive, it can be used on multiple genomes to evaluate gene expression across many different conditions, tissues and cell types. Although many tools exist to map and compare RNA-Seq at the genomics level, few web-based tools are dedicated to making data generated for individual genomic analysis accessible and reusable at a gene-level scale for comparative analysis between genes, across different genomes and meta-analyses. Results: To address this challenge, we revamped the comparative gene …

A Phytobacterial Tir Domain Effector Manipulates Nad+ To Promote Virulence, Samuel Eastman, Thomas Smith, Mark A. Zaydman, Panya Kim, Samuel Martinez, Neha Damaraju, Aaron Diantonio, Jeffrey Milbrandt, Thomas Clemente, James R. Alfano, Ming Guo

Department of Agronomy and Horticulture: Faculty Publications

The Pseudomonas syringae DC3000 type III effector HopAM1 suppresses plant immunity and contains a Toll/interleukin-1 receptor (TIR) domain homologous to immunity-related TIR domains of plant nucleotide-binding leucine-rich repeat receptors that hydrolyze nicotinamide adenine dinucleotide (NAD+) and activate immunity. In vitro and in vivo assays were conducted to determine if HopAM1 hydrolyzes NAD+ and if the activity is essential for HopAM1’s suppression of plant immunity and contribution to virulence. HPLC and LC-MS were utilized to analyze metabolites produced from NAD+ by HopAM1 in vitro and in both yeast and plants. Agrobacterium-mediated transient expression and in planta inoculation assays were performed to …

Predicting Munsell Color For Turfgrass Leaves, William L. Berndt, Roch E. Gaussoin

Department of Agronomy and Horticulture: Faculty Publications

Linking turfgrass color to hue, value, chroma (H V/C) in the Munsell Plant Tissue Color Book is a visual comparison process for specifying and communicating plant color. If subjectivity of visual comparison can be mitigated, then accuracy of color matching may be improved. Research was conducted to develop an algorithm predicting H V/C from CIE-xyY color (xyY) in digital images of leaves of four turfgrasses. First, value-chroma (V/C) arrays for Munsell hue groups 5Y, 2.5GY, 5GY, 7.5GY, 10GY, and 2.5G were converted to xyY. Next, chromaticity (xy) plots from each array were fitted with …

Targeting Transgenic Proteins To The Mitochondria In Plants, Susan Qudus, Jeffrey P. Mower

Honors Program: Senior Projects (Public)

Cytoplasmic male sterility (CMS) in plants can convert an individual into female through sterilization of the male reproductive parts, creating an inability to produce functional pollen. In nature, this is advantageous for genetic diversity and producing progeny with unique genes that may be beneficial in evolution. For agriculture, this method is preferential for selection of desirable traits in crops when cross breeding. CMS is typically caused by genes in the mitochondrial DNA (mtDNA), such as novel open reading frames (ORFs) that form after imperfect repair of double-stranded breaks in the mtDNA. Specific CMS genes vary among various plant species. It …

Field Validation Of A Farmer Supplied Data Approach To Close Soybean Yield Gaps In The Us North Central Region, José F. Andrade, Spyridon Mourtzinis, Juan I. Rattalino Edreira, Shawn P. Conley, John Gaska, Herman J. Kandel, Laura E. Lindsey, Seth Naeve, Scott Nelson, Maninder P. Singhi, Laura J. Thompson, James E. Specht, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

CONTEXT: Producer-reported data can be used to identify suites of management practices that lead to higher yield and profit. However, a rigorous validation of the approach in relation to its potential impact on farmer yield and profit is lacking.

OBJECTIVE: This study aimed to validate a producer-data approach on its capability to guide on-farm evaluation of management practices with greatest potential for increasing producer yield and profit. We show proof of concept using soybean in the North Central US region as a case study.

METHODS: We used a combination of regression tree analysis and a spatial framework to determine practices …

Ecological Intensification With Soil Health Practices Demonstrates Positive Impacts On Multiple Soil Properties: A Large-Scale Farmer-Led Experiment, Fernanda Souza Krupek, Daren D. Redfearn, Kent M. Eskridge, Andrea Basche

Department of Agronomy and Horticulture: Faculty Publications

Improving soil health is critical to reversing trends of soil degradation and is of increasing interest to a range of stakeholders including policymakers, agricultural industry leaders, food companies, and farmers. Crop and soil management practices focused on ecological functions can be effective in restoring fundamental biological, chemical and physical soil properties. The call for ecological intensification of agricultural systems has the potential to improve soil health and input-use efficiency. In this study, we developed a framework to classify spatial and temporal ecological intensification with soil health practices: tillage, crop rotation, cover crop, organic amendment, and crop-livestock integration. We applied this …

Charles A. Francis, Abstracts And Publications [1969--2022], December 2022, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Contents

I. Journal articles (218), p.1

II. Book reviews (60), p. 18

III. Published abstracts (213), p. 23

IV. Books (23), p. 40

V. Chapters in books (81), p. 42

VI. Symposium or workshop proceedings (127), p. 50

VII. Symposia or extension publications edited (31), p. 62

VIII. Miscellaneous (522), p. 65

Improving Taxonomic Delimitation Of Fungal Species In The Age Of Genomics And Phenomics, Ashley Stengel, Kimberly M. Stanke, Amanda C. Quattrone, Joshua R. Herr

Department of Plant Pathology: Faculty Publications

Species concepts have long provided a source of debate among biologists. These lively debates have been important for reaching consensus on how to communicate across scientific disciplines and for advancing innovative strategies to study evolution, population biology, ecology, natural history, and disease epidemiology. Species concepts are also important for evaluating variability and diversity among communities, understanding biogeographical distributions, and identifying causal agents of disease across animal and plant hosts. While there have been many attempts to address the concept of species in the fungi, there are several concepts that have made taxonomic delimitation especially challenging. In this review we discuss …

Determining The Novel Pathogen Neodothiora Populina As The Causal Agent Of The Aspen Running Canker Disease In Alaska, Loretta M. Winton, Gerard C. Adams, Roger W. Ruess

Department of Plant Pathology: Faculty Publications

Neodothiora populina Crous, G.C. Adams & Winton was determined to be a new pathogen of trembling aspen (Populus tremuloides) growing in Alaska, based on completion of Koch's Postulates in replicated forest and growth chamber inoculation trials. The pathogen is responsible for severe damage and widespread rapid mortality of sapling to mature aspen (≥ 80 years) in the boreal forests of interior Alaska, due to large diffuse annual (1–2 years) cankers. Isolation of the pathogen was challenging, and identification based on cultural characters was difficult. Fruiting bodies were not found on wild diseased trees, but erumpent pycnidia were found …

Characterization Of Salt And Drought Tolerance In Sunflower (Helianthus Annuus L.), Sevgi Saylak

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Consequent to the increasing world population, food sources are needed to be increased to meet the nutritional needs. However, due to natural processes and agricultural activities, the most destructive environmental factors that limit crop production, soil salinity, and drought-exposed areas are growing. As one of the major oilseed crops, sunflower (Helianthus annuus L.), is considered to be moderately tolerant to salt and drought. Although it can grow in arid to semi-arid regions, increasing salinity and drought might adversely affect sunflower production. This study aimed to investigate several sunflower germplasms' morphological responses to salt and drought stresses. For this purpose, …

Identification Of Beneficial And Detrimental Bacteria Impacting Sorghum Responses To Drought Using Multi-Scale And Multisystem Microbiome Comparisons, Mingsheng Qi, Jeffrey C. Berry, Kira W. Veley, Lily O'Connor, Omri M. Finkel, Isai Salas-González, Molly Kuhs, Julietta Jupe, Emily Holcomb, Tijana Glavina Del Rio, Cody Creech, Peng Liu, Susannah G. Tringe, Jeffery L. Dangl, Daniel P. Schachtman, Rebecca S. Bart

Department of Agronomy and Horticulture: Faculty Publications

Drought is a major abiotic stress limiting agricultural productivity. Previous field-level experiments have demonstrated that drought decreases microbiome diversity in the root and rhizosphere. How these changes ultimately affect plant health remains elusive. Toward this end, we combined reductionist, transitional and ecological approaches, applied to the staple cereal crop sorghum to identify key root-associated microbes that robustly affect drought-stressed plant phenotypes. Fifty-three Arabidopsis-associated bacteria were applied to sorghum seeds and their effect on root growth was monitored. Two Arthrobacter strains caused root growth inhibition (RGI) in Arabidopsis and sorghum. In the context of synthetic communities, Variovorax strains were able to …

Temporal Changes In The Nutrient Content Of Cattle Dung In The Nebraska Sandhills Ecosystem, Amanda Shine, Martha Mamo, Gandura O. Abagandura, Walter Schacht, Jerry Volesky

Department of Agronomy and Horticulture: Faculty Publications

Dung excreted by cattle composes a significant portion of the nutrient inputs in a grazed ecosystem and can have wide-ranging effects on soil properties and vegetation. However, little research has been conducted on the nutrient dynamics of excreted dung in situ that has not been disturbed prior to field sampling. In this study, we analyzed 294 dung pats (1–24 days old) collected from a Nebraska Sandhills meadow to determine water-extractable organic carbon (WEOC), water-extractable nitrogen (WEN), water-extractable phosphorus (WEP), and percent dry matter (DM) changes over time. In addition, we investigated if sample handling - frozen storage – and the …