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Articles 31 - 35 of 35
Full-Text Articles in Plant Biology
Cooperation And Punishment In The Arbuscular Mycorrhizal Symbiosis: Implications For Resource Exchange & Biological Market Dynamics, Jerry A. Mensah
Cooperation And Punishment In The Arbuscular Mycorrhizal Symbiosis: Implications For Resource Exchange & Biological Market Dynamics, Jerry A. Mensah
Electronic Theses and Dissertations
The arbuscular mycorrhizal (AM) symbiosis is arguably the world’s most abundant and important mutualism, and brings together the roots of the majority of land plants and AM fungi to great mutual advantage. The AM symbiosis can increase the uptake of nutrients, such as phosphorus (P) and nitrogen (N), and improves the abiotic and biotic stress resistance of the host plant. AM fungi have the potential to act as biofertilizers and bioprotectors in sustainable agriculture. However, despite its significance, the mechanisms that control the resource exchange between both partners in the arbuscular mycorrhizal symbiosis are largely unknown. The main aim of …
Role Of Silencing Rna Fgsir34 In Fusarium Graminearum's Pathogenicity To Wheat, Subha Dahal
Role Of Silencing Rna Fgsir34 In Fusarium Graminearum's Pathogenicity To Wheat, Subha Dahal
Electronic Theses and Dissertations
Fusarium graminearum is an ascomycetous fungal pathogen that causes Fusarium head blight (FHB) disease in wheat and other cereal grains. Mycotoxin produced by the fungus, predominantly deoxynivalenol (DON), is considered as an important virulence factor for the spread of disease. Our previous study of a Dicer-like 2 knockdown mutant has led to our hypothesis that a silencing RNA, fgsiR34, might play a key role in regulating DON biosynthesis and some other virulent factors. To test this hypothesis, we generated an fgsiR34 over-expressing mutant (ΔfgsiR34+) using Inverse Repeat Transgene method and studied the pathogenicity of the mutant in …
Effects Of Stratification, Warm Treatment, And Mechanical And Acid Scarification On The Emergence Of Yellow-Flowered Alfalfa (Medicago Sativa Subsp. Falcata) Seeds, Diane Narem
The Journal of Undergraduate Research
Yellow-flowered alfalfa (YFA) has great potential to improve rangelands in the Northern Plains. Greater than 99% of YFA seeds from soil were viable but less than 4% germinated under standard laboratory conditions. The objective was to determine if low germination was due to physical or physiological seed dormancy. The experiment utilized a random complete block design with two trials, eight replications per trial. Each replication consisted of ten treatments plus a control, four stratification treatments consisting of a combination of two temperatures (-5°C, -20°C) and two durations (48 hr, 72 hr), one warm treatment (4 min, 60°C), four sandpaper scarification …
Detecting Shifts In Soil Microbial Community Structure And Function Post Landspread Of Manure Or Biosolids Containing Antimicrobial Chemicals, Kelly Lehnert, Sharon A. Clay, Susan Gibson, Volker Brozel
Detecting Shifts In Soil Microbial Community Structure And Function Post Landspread Of Manure Or Biosolids Containing Antimicrobial Chemicals, Kelly Lehnert, Sharon A. Clay, Susan Gibson, Volker Brozel
Agronomy, Horticulture and Plant Science Faculty Publications
Soil microbial diversity and community interaction play an indispensable role in 2,4-dichorophenoxyacetic acid (2,4-D) herbicide degradation. The addition of manure or municipal waste biosolids through landspreading may alter soil community structure and function if these materials contain antimicrobial chemicals like chlortetracycline (CTC), administered in livestock feed to promote animal growth and health or tetracycline (TET) utilized in human health. In this study, soil applied with manure collected from pigs fed standard CTC levels was compared to soil containing manure from control pigs fed no CTC, and a comparison of soil applied with biosolids containing TET or without TET to distinguish …
Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman
Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman
The Journal of Undergraduate Research
HVCR21 is a barley protein known to be cold regulated at the mRNA level. However, its function as well as the affect of low temperature on its translation are unknown. The purpose of this project was to engineer E. coli to express recombinant HVCR21. PGR primers were designed for the 5' and 3' ends of the HVCR21 coding region. The primers were also designed with a 5' SacI restriction site and a 3' Pst I restriction site. After ligation of the PCR product into the pCR4-TOPO vector, bacteria were transformed and plated and the successful transformant verified by PCR. The …