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South Dakota State University

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Articles 31 - 35 of 35

Full-Text Articles in Plant Biology

Evaluation And Identification Of Soybean Aphid Resistance Sources And Mapping Of Soybean Aphid Resistance Loci In Early Maturing Soybean Germplasm Accessions, Siddhi Jeewan Bhusal Jan 2016

Evaluation And Identification Of Soybean Aphid Resistance Sources And Mapping Of Soybean Aphid Resistance Loci In Early Maturing Soybean Germplasm Accessions, Siddhi Jeewan Bhusal

Electronic Theses and Dissertations

Soybean aphid (Aphis glycines Matsumura) has been a major pest of soybean [Glycine max (L.) Merr.] in North America particularly in the northern United States and three Canadian provinces. At least four biotypes of soybean aphid have been confirmed in the United States. Identification of soybean aphid resistance sources in early-maturing soybeans and genetic characterization of new sources of resistance will facilitate to expand the gene pool of soybean aphid resistance and thus will help to develop soybean aphid resistant cultivars. To identify new sources of resistance in early maturing soybeans, 330 soybean germplasm accessions from Maturity Group (MG) I, …


Cooperation And Punishment In The Arbuscular Mycorrhizal Symbiosis: Implications For Resource Exchange & Biological Market Dynamics, Jerry A. Mensah Jan 2016

Cooperation And Punishment In The Arbuscular Mycorrhizal Symbiosis: Implications For Resource Exchange & Biological Market Dynamics, Jerry A. Mensah

Electronic Theses and Dissertations

The arbuscular mycorrhizal (AM) symbiosis is arguably the world’s most abundant and important mutualism, and brings together the roots of the majority of land plants and AM fungi to great mutual advantage. The AM symbiosis can increase the uptake of nutrients, such as phosphorus (P) and nitrogen (N), and improves the abiotic and biotic stress resistance of the host plant. AM fungi have the potential to act as biofertilizers and bioprotectors in sustainable agriculture. However, despite its significance, the mechanisms that control the resource exchange between both partners in the arbuscular mycorrhizal symbiosis are largely unknown. The main aim of …


Effects Of Stratification, Warm Treatment, And Mechanical And Acid Scarification On The Emergence Of Yellow-Flowered Alfalfa (Medicago Sativa Subsp. Falcata) Seeds, Diane Narem Jan 2009

Effects Of Stratification, Warm Treatment, And Mechanical And Acid Scarification On The Emergence Of Yellow-Flowered Alfalfa (Medicago Sativa Subsp. Falcata) Seeds, Diane Narem

The Journal of Undergraduate Research

Yellow-flowered alfalfa (YFA) has great potential to improve rangelands in the Northern Plains. Greater than 99% of YFA seeds from soil were viable but less than 4% germinated under standard laboratory conditions. The objective was to determine if low germination was due to physical or physiological seed dormancy. The experiment utilized a random complete block design with two trials, eight replications per trial. Each replication consisted of ten treatments plus a control, four stratification treatments consisting of a combination of two temperatures (-5°C, -20°C) and two durations (48 hr, 72 hr), one warm treatment (4 min, 60°C), four sandpaper scarification …


Detecting Shifts In Soil Microbial Community Structure And Function Post Landspread Of Manure Or Biosolids Containing Antimicrobial Chemicals, Kelly Lehnert, Sharon A. Clay, Susan Gibson, Volker Brozel Nov 2007

Detecting Shifts In Soil Microbial Community Structure And Function Post Landspread Of Manure Or Biosolids Containing Antimicrobial Chemicals, Kelly Lehnert, Sharon A. Clay, Susan Gibson, Volker Brozel

Agronomy, Horticulture and Plant Science Faculty Publications

Soil microbial diversity and community interaction play an indispensable role in 2,4-dichorophenoxyacetic acid (2,4-D) herbicide degradation. The addition of manure or municipal waste biosolids through landspreading may alter soil community structure and function if these materials contain antimicrobial chemicals like chlortetracycline (CTC), administered in livestock feed to promote animal growth and health or tetracycline (TET) utilized in human health. In this study, soil applied with manure collected from pigs fed standard CTC levels was compared to soil containing manure from control pigs fed no CTC, and a comparison of soil applied with biosolids containing TET or without TET to distinguish …


Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman Jan 2003

Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman

The Journal of Undergraduate Research

HVCR21 is a barley protein known to be cold regulated at the mRNA level. However, its function as well as the affect of low temperature on its translation are unknown. The purpose of this project was to engineer E. coli to express recombinant HVCR21. PGR primers were designed for the 5' and 3' ends of the HVCR21 coding region. The primers were also designed with a 5' SacI restriction site and a 3' Pst I restriction site. After ligation of the PCR product into the pCR4-TOPO vector, bacteria were transformed and plated and the successful transformant verified by PCR. The …