Plant Biology Commons^™

Editorial: New Insights Into Mechanisms Of Epigenetic Modifiers In Plant Growth And Development, Volume Ii, Ming Luo, Tomasz Jacek Sarnowski, Marc Libault, Gabino Ríos, Jean-Benoit Charron, Nitin Mantri, Shoudong Zhang

Department of Agronomy and Horticulture: Faculty Publications

As we have learned, chromatin modifications, including histone modifications and DNA methylation, play a key role in plant development (Ng and Bird, 1999). However, accumulated evidence shows that, besides chromatin biochemical modifications, other epigenetic regulations such as chromatin architecture also function at a pivot point to regulate plant development (Zhang et al., 2021). In this Research Topic, five research papers describe multiple developmental facets mediated by different epigenetic mechanisms besides histone modifications. These manuscripts report experimental evidence or summarize recent advances in epigenetic regulations of some important developmental genes or development-related mechanisms. This Research Topic allows readers to learn of …

A Complete Telomere-To-Telomere Assembly Of The Maize Genome, Jian Chen, Zijian Wang, Kaiwen Tan, Wei Huang, Junpeng Shi, Tong Li, Jiang Hu, Kai Wang, Chao Wang, Beibei Xin, Haiming Zhao, Weibin Song, Matthew B. Hufford, James C. Schnable, Weiwei Jin, China Agricultural University, Hainan Yazhou Bay Seed Laboratory

Department of Agronomy and Horticulture: Faculty Publications

A complete telomere-to-telomere (T2T) finished genome has been the long pursuit of genomic research. Through generating deep coverage ultralong Oxford Nanopore Technology (ONT) and PacBio HiFi reads, we report here a complete genome assembly of maize with each chromosome entirely traversed in a single contig. The 2,178.6 Mb T2T Mo17 genome with a base accuracy of over 99.99% unveiled the structural features of all repetitive regions of the genome. There were several super-long simple-sequence-repeat arrays having consecutive thymine–adenine– guanine (TAG) tri-nucleotide repeats up to 235 kb. The assembly of the entire nucleolar organizer region of the 26.8 Mb array with …

Genome-Wide Association Analysis For Emergence Of Deeply Sown Rice (Oryza Sativa) Reveals Novel Aus-Specific Phytohormone Candidate Genes For Adaptation To Dry-Direct Seeding In The Field, Sandeep A. Sakhale, Shailesh Yadav, Lindsay V. Clark, Alexander E. Lipka, Arvind Kumar, Erik J. Sacks

Department of Agronomy and Horticulture: Faculty Publications

Dry direct-seeded rice (dry-DSR) is typically sown deeply to circumvent the need for irrigation, and thus seedling emergence is a crucial trait affecting plant stand and yield. To breed elite cultivars that use less water and are climate-resilient, an understanding of the genomic regions and underlying genes that confer emergence in deeply sown dry-DSR would be highly advantageous. A combined diversity panel of 470 rice accessions (RDP1 plus aus subset of 3K RGP) was evaluated with 2.9 million single nucleotide polymorphisms (SNPs) to identify associations with dry-DSR traits in the field and component traits in a controlled-environment experiment. Using genome-wide …

Do Cover Crop Mixtures Improve Soil Physical Health More Than Monocultures?, Humberto Blanco-Canqui

Department of Agronomy and Horticulture: Faculty Publications

Rationale and Purpose — Adding multispecies cover crop (CC) mixtures could diversify the current simplified crop rotations and enhance soil health more than monoculture CCs. Further, CC mixtures with diverse plant species could adapt better to changing climatic and environmental conditions than monoculture CCs. However, our current understanding of the soil benefits of CC mixtures is still limited. This review discussed whether CC mixtures are better than monoculture CCs to improve soil physical health.

Methods — All studies published up to May 25, 2023, comparing soil physical properties between CC mixtures and their constituents grown as monocultures were searched in …

2018–2019 Field Seasons Of The Maize Genomes To Fields (G2f) G X E Project, Dayane Dayane Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Bridget A. Mcfarland, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint‑Garcia, Candice N. Hirsch, Elizabeth Hood, David C. Hooker, Joseph E. Knoll, Judith M. Kolkman, Sanzhen Liu, John Mckay, Richard Minyo, Danilo E. Moreta, Seth C. Murray, Rebecca Nelson, James C. Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Randall J. Wisser, Wenwei Xu31, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Objectives This report provides information about the public release of the 2018–2019 Maize G X E project of the Genomes to Fields (G2F) Initiative datasets. G2F is an umbrella initiative that evaluates maize hybrids and inbred lines across multiple environments and makes available phenotypic, genotypic, environmental, and metadata information. The initiative understands the necessity to characterize and deploy public sources of genetic diversity to face the challenges for more sustainable agriculture in the context of variable environmental conditions.

Data description Datasets include phenotypic, climatic, and soil measurements, metadata information, and inbred genotypic information for each combination of location and year. …

Induction And Suppression Of Gene Silencing In Plants By Nonviral Microbes, Eric Parperides, Kaoutar El Mounadi, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Gene silencing is a conserved mechanism in eukaryotes that dynamically regulates gene expression. In plants, gene silencing is critical for development and for maintenance of genome integrity. Additionally, it is a critical component of antiviral defence in plants, nematodes, insects, and fungi. To overcome gene silencing, viruses encode effectors that suppress gene silencing. A growing body of evidence shows that gene silencing and suppression of silencing are also used by plants during their interaction with nonviral pathogens such as fungi, oomycetes, and bacteria. Plant–pathogen interactions involve trans-kingdom movement of small RNAs into the pathogens to alter the function of genes …

Induction And Suppression Of Gene Silencing In Plants By Nonviral Microbes, Eric Parperides, Kaoutar El Mounadi, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Gene silencing is a conserved mechanism in eukaryotes that dynamically regulates gene expression. In plants, gene silencing is critical for development and for maintenance of genome integrity. Additionally, it is a critical component of antiviral defence in plants, nematodes, insects, and fungi. To overcome gene silencing, viruses encode effectors that suppress gene silencing. A growing body of evidence shows that gene silencing and suppression of silencing are also used by plants during their interaction with nonviral pathogens such as fungi, oomycetes, and bacteria. Plant–pathogen interactions involve trans-kingdom movement of small RNAs into the pathogens to alter the function of genes …

Creeper Legume, In Conjunction With Biochar, Is A Potential Tool To Minimize Soil Erosion, Sujani De Silva, Priyantha Indralal Yapa, Kushani Mahatantila, Saurav Das, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Accelerated soil erosion and landslides are destructive consequences of road development and intensive agriculture in the central highlands of Sri Lanka. Properly designed vegetation covers can play a vital role in erosion control. Identifying a plant that can adapt to eroded land with a low-nutrient supply is critical for natural erosion management. A perennial creeper legume, Vigna marina, adaptable for marginal lands and used to control soil erosion in Australia, was introduced to Sri Lanka via the 2004 tsunami. The objective of this study was to assess V. marina under five different soil substrates, including a reference treatment (RT) …

Effectiveness Of Tadreb-B1 And 1-Feh W3 Kasp Markers In Spring And Winter Wheat Populations For Marker-Assisted Selection To Improve Drought Tolerance, Shamseldeen Eltaher, Mostafa Hashem, Asmaa A. M. Ahmed, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Due to the advances in DNA markers, kompetitive allele-specific PCR (KASP) markers could accelerate breeding programs and genetically improve drought tolerance. Two previously reported KASP markers, TaDreb-B1 and 1-FEH w3, were investigated in this study for the markerassisted selection (MAS) of drought tolerance. Two highly diverse spring and winter wheat populations were genotyped using these two KASP markers. The same populations were evaluated for drought tolerance at seedling (drought stress) and reproductive (normal and drought stress) growth stages. The single-marker analysis revealed a high significant association between the target allele of 1-FEH w3 and drought susceptibility in the spring …

Long-Term Conservation And Conventional Tillage Systems Impact Physical And Biochemical Soil Health Indicators In A Corn–Soybean Rotation, Anuoluwa O. Sangotayo, Jemila Chellappa, Udayakumar Sekaran, Sangeeta Bansal, Padma Angmo, Paul J. Jasa, Sandeep Kumar, Javed Iqbal

Department of Agronomy and Horticulture: Faculty Publications

Agricultural management practices tend to influence soil structure stabilization, mediating many physical, chemical, and biological processes in soils. Therefore, understanding the long-term effects of management practices on various soil health indicators is crucial to develop sustainable agricultural practices. This study aimed to assess the long-term conventional and conservation tillage effects on soil physical (aggregates) and biochemical (soil organic carbon [SOC], enzymes, and microbial biomass) parameters under a range of tillage practices in a corn–soybean rotation in Nebraska. The experiment was conducted at two locations as follows: (1) Concord site (36 years) with the three treatments: no-till (NT), disk, and moldboard …

Hybrid Allele‑Specific Chip‑Seq Analysis Identifies Variation In Brassinosteroid‑Responsive Transcription Factor Binding Linked To Traits In Maize, Thomas Hartwig, Michael Banf, Gisele Passaia Prietsch, Jia‑Ying Zhu, Isabel Mora‑Ramírez, Jos H. M. Schippers, Samantha J. Snodgrass, Arun S. Seetharam, Bruno Huettel, Judith M. Kolkman, Jiashi Yang, Julia Engelhorn, Zhi‑Yong Wang

Department of Agronomy and Horticulture: Faculty Publications

Background: Genetic variation in regulatory sequences that alter transcription factor (TF) binding is a major cause of phenotypic diversity. Brassinosteroid is a growth hormone that has major effects on plant phenotypes. Genetic variation in brassinosteroidresponsive cis-elements likely contributes to trait variation. Pinpointing such regulatory variations and quantitative genomic analysis of the variation in TF-target binding, however, remains challenging. How variation in transcriptional targets of signaling pathways such as the brassinosteroid pathway contributes to phenotypic variation is an important question to be investigated with innovative approaches.

Results: Here, we use a hybrid allele-specific chromatin binding sequencing (HASChseq) approach and …

A Deep Learning Framework For Processing And Classification Of Hyperspectral Rice Seed Images Grown Under High Day And Night Temperatures, Víctor Díaz-Martínez, Jairo Orozco-Sandoval, Vidya Manian, Balpreet K. Dhatt, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

A framework combining two powerful tools of hyperspectral imaging and deep learning for the processing and classification of hyperspectral images (HSI) of rice seeds is presented. A seed-based approach that trains a three-dimensional convolutional neural network (3D-CNN) using the full seed spectral hypercube for classifying the seed images from high day and high night temperatures, both including a control group, is developed. A pixel-based seed classification approach is implemented using a deep neural network (DNN). The seed and pixel-based deep learning architectures are validated and tested using hyperspectral images from five different rice seed treatments with six different high temperature …

Entropy And Energy Profiles Of Chemical Reactions, Johannes C. B. Dietschreit, Dennis J. Diestler, Rafael Gómez-Bombarelli

Department of Agronomy and Horticulture: Faculty Publications

The description of chemical processes at the molecular level is often facilitated by use of reaction coordinates, or collective variables (CVs). The CV measures the progress of the reaction and allows the construction of profiles that track the evolution of a specific property as the reaction progresses. Whereas CVs are routinely used, especially alongside enhanced sampling techniques, links between profiles and thermodynamic state functions and reaction rate constants are not rigorously exploited. Here, we report a unified treatment of such reaction profiles. Tractable expressions are derived for the free-energy, internal-energy, and entropy profiles as functions of only the CV. We …

Divergent Metabolic Changes In Rhizomes Of Lowland And Upland Switchgrass (Panicum Virgatum) From Early Season Through Dormancy Onset, Nathan A. Palmer, Gautam Sarath, Michael J. Bowman, Aaron J. Saathoff, Serge J. Edmé, Robert B. Mitchell, Christian M. Tobias, Soundararajan Madhavan, Erin D. Scully, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

High-biomass-yielding southerly adapted switchgrasses (Panicum virgatum L.) frequently suffer from unpredictable winter hardiness at more northerly sites arising from damage to rhizomes that prevent effective spring regrowth. Previously, changes occurring over the growing season in rhizomes sampled from a cold-adapted tetraploid upland cultivar, Summer, demonstrated a role for abscisic acid (ABA), starch accumulation, and transcriptional reprogramming as drivers of dormancy onset and potential keys to rhizome health during winter dormancy. Here, rhizome metabolism of a high-yielding southerly adapted tetraploid switchgrass cultivar, Kanlow—which is a significant source of genetics for yield improvement—was studied over a growing season at a northern …

Herbicide Drift Vs. Crop Resilience – The Influence Of Micro-Rates, Milan Brankov, Bruno Canella Vieira, Miloš Rajković, Milena Simić, Vukadinović Jelena, Violeta Mandić, Vesna Dragičević

Department of Agronomy and Horticulture: Faculty Publications

A greenhouse study was conducted to test the effects of low herbicide dose exposure on different crops measuring visible damages, plant height, leaf area, and dry matter. Seven crops were tested: lettuce (Lactuca sativa L.) cv. Novosadska majska maslena, oil pumpkin (Cucurbita maxima Duch) cv. Olivija, oilseed rape (Brassica napus L.) cv. NS Ras, pepper (Capsicum annuum L.) cv. Kurtovska kapija, soybean (Glycine max (L.) Merr) cv. ZP Laura, sunflower (Helianthus annuus L.) cv. NS Kruna, and tomato (Solanum lycopersicum L.) cv. Dunavski Rubin. Herbicide dicamba in the range of 0.14 to 1 …

Late Postemergence Glufosinate-Based Programs For Glyphosate-Resistant Palmer Amaranth Control In Dicamba/Glufosinate/Glyphosate-Resistant Soybean, Vipan Kumar, Jatinder S. Aulakh, Rui Liu, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Glyphosate-resistant (GR) Palmer amaranth (Amaranthus palmeri S. Watson) is widespread in the Central Great Plains. Introduction of newly developed dicamba/glufosinate/glyphosate (DGG)-resistant soybean varieties allows postemergence (POST) applications of dicamba and glufosinate for in-season control of GR Palmer amaranth. Limited information exists on the effectiveness of glufosinate applied late-POST for tall (70–90 cm) GR Palmer amaranth control in DGG-resistant soybean. The objectives of this study were to (1) determine the effectiveness of late-POST glufosinate-based programs for GR Palmer amaranth control, and (2) determine the impact of those programs on soybeans grain yields. Ten glufosinatebased programs were tested in a field …

A Leaf-Level Spectral Library To Support High-Throughput Plant Phenotyping: Predictive Accuracy And Model Transfer, Nuwan K. Wijewardane, Huichun Zhang, Jinliang Yang, James C. Schnable, Daniel P. Schachtman, Yufeng Ge

Department of Agronomy and Horticulture: Faculty Publications

Leaf-level hyperspectral reflectance has become an effective tool for high-throughput phenotyping of plant leaf traits due to its rapid, low-cost, multi-sensing, and non-destructive nature. However, collecting samples for model calibration can still be expensive, and models show poor transferability among different datasets. This study had three specific objectives: first, to assemble a large library of leaf hyperspectral data (n=2460) from maize and sorghum; second, to evaluate two machine-learning approaches to estimate nine leaf properties (chlorophyll, thickness, water content, nitrogen, phosphorus, potassium, calcium, magnesium, and sulfur); and third, to investigate the usefulness of this spectral library for predicting external datasets …

Quantifying The Relationship Between Arbuscular Mycorrhizal Fungi And A Native And Invasive Thistle, Nicole Baker, Lucia Vera, Ian Rosales

2023 Midwest Ecology & Evolution Conference

Arbuscular mycorrhizal fungi (AMF) associate with 80% of vascular plant roots and are an important part of nutrient exchange. Our research quantified the relationship between (AMF) and native Pasture Thistle (Cirsium discolor) and invasive Canada Thistle (Cirsium arvense). We hypothesized that Pasture Thistle would have a stronger relationship with AMF than Canada Thistle because of the degraded mutualism hypothesis, which states that invasive species have reduced relationships with AMF. For our experiment, we grew inoculated and non-inoculated plants at 10 different levels of soil phosphorus concentrations. Pasture Thistle and Canada Thistle were both facultatively dependent upon …

Effects Of Heavy Metal Uptake On Growth, Chlorophyll Content, And Calcium Oxalate Crystals In Lemna Minor (Duckweed), Olivia F. Miller

Undergraduate Honors Thesis Projects

Lemna minor L., duckweed, is a common aquatic plant used for the phytoremediation of wastewater. Heavy metal contamination poses a significant issue, and numerous studies have been conducted on the efficiency of L. minor’s hyperaccumulation ability of these metals. Calcium oxalate crystals are an important aspect of the growth and development of L. minor, but how they are influenced by the uptake of heavy metal has not been extensively studied. This study aims to determine the effects that lead, cadmium, and copper have on the formation of calcium oxalate crystals, vegetative growth, and chlorophyll a and b content on L. …

Predicting Site‑Specific Economic Optimal Nitrogen Rate Using Machine Learning Methods And On‑Farm Precision Experimentation, Alfonso De Lara, Taro Mieno, Joe D. Luck, Laila A. Puntel

Department of Agronomy and Horticulture: Faculty Publications

Applying at the economic optimal nitrogen rate (EONR) has the potential to increase nitrogen (N) fertilization efficiency and profits while reducing negative environmental impacts. On-farm precision experimentation (OFPE) provides the opportunity to collect large amounts of data to estimate the EONR. Machine learning (ML) methods such as generalized additive models (GAM) and random forest (RF) are promising methods for estimating yields and EONR. Twenty OFPE N trials in wheat and barley were conducted and analyzed with soil, terrain and remote-sensed variables to address the following objectives: (1) to quantify the spatial variability of winter crops yield and the yield response …

A Plea For Scale, And Why It Matters For Invasive Species Management, Biodiversity And Conservation, Nicholas A. Mcmillan, Samuel D. Fuhlendorf, Craig A. Davis, Robert G. Hamilton, Landon. K. Neumann, Samantha M. Cady

Department of Agronomy and Horticulture: Faculty Publications

1. Invasive species are suspected to be major contributors to biodiversity declines worldwide. Counterintuitively, however, invasive species effects are likely scale dependent and are hypothesized to be positively related to biodiversity at large spatial scales. Past studies investigating the effect of invasion on biodiversity have been mostly conducted at small scales (<100 m²) that cannot represent large dynamic landscapes by design. Therefore, replicated experimental evidence supporting a negative effect of invasive plants on biodiversity is lacking across many landscape types, including large grasslands.

2. We collected data across eight large (333–809 ha) grassland landscapes managed with pyric herbivory—that is the recoupling …

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Soil Depth And Geographic Distance Modulate Bacterial Β-Diversity In Deep Soil Profiles Throughout The U.S. Corn Belt, Lucas Dantas Lopes, Stephanie L. Futrell, Emily E. Wright, Gerasimos J. Danalatos, Michael J. Castellano, Tony J. Vyn, Sotirios V. Archontoulis, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Understanding how microbial communities are shaped across spatial dimensions is of fundamental importance in microbial ecology. However, most studies on soil biogeography have focused on the topsoil microbiome, while the factors driving the subsoil microbiome distribution are largely unknown. Here we used 16S rRNA amplicon sequencing to analyse the factors underlying the bacterial β-diversity along vertical (0–240 cm of soil depth) and horizontal spatial dimensions (~500,000 km²) in the U.S. Corn Belt. With these data we tested whether the horizontal or vertical spatial variation had stronger impacts on the taxonomic (Bray-Curtis) and phylogenetic (weighted Unifrac) β-diversity. Additionally, we …

Graph Convolutional Network Using Adaptive Neighborhood Laplacian Matrix For Hyperspectral Images With Application To Rice Seed Image Classification, Jairo Orozco, Vidya Manian, Estefania Alfaro, Harkamal Walia, Balpreet K. Dhatt

Department of Agronomy and Horticulture: Faculty Publications

Graph convolutional neural network architectures combine feature extraction and convolutional layers for hyperspectral image classification. An adaptive neighborhood aggregation method based on statistical variance integrating the spatial information along with the spectral signature of the pixels is proposed for improving graph convolutional network classification of hyperspectral images. The spatial-spectral information is integrated into the adjacency matrix and processed by a single-layer graph convolutional network. The algorithm employs an adaptive neighborhood selection criteria conditioned by the class it belongs to. Compared to fixed window-based feature extraction, this method proves effective in capturing the spectral and spatial features with variable pixel neighborhood …

Harvest Aids Did Not Advance Maturity Of Non-Shatter Pennycress, Julija A. Cubins, Samantha Wells, Russ W. Gesch, Gregg A. Johnson, Maninder K. Walia, Ratan Chopra, M. David Marks, Rebecca D. Swenson, Katherine Anna Frels

Department of Agronomy and Horticulture: Faculty Publications

Reliance on summer annual crops in the Upper Midwest results in fallow land from late fall through early spring, providing opportunities to integrate winter crops, such as pennycress (Thlapsi arvense L.), onto the landscape. Pennycress agronomics have primarily been studied using unimproved wild-type lines prone to seed shatter, resulting in significant yield loss if not harvested early. However, high plant and seed moisture complicates harvest and seed storage. A new breeding line with a reducedshatter mutation made it possible to use harvest aids to reduce plant moisture without the risk of seed loss. The objectives of this study were …

A Role For Heritable Transcriptomic Variation In Maize Adaptation To Temperate Environments, Guangchao Sun, Huihui Yu, Peng Wang, Martha Lopez‑Guerrero, Ravi V. Mural, Olivier N. Mizero, Marcin Grzybowski, Baoxing Song, Karin V. Van Dijk, Daniel P. Schachtman, Chi Zhang, James C. Schnable

Department of Agronomy and Horticulture: Faculty Publications

Background: Transcription bridges genetic information and phenotypes. Here, we evaluated how changes in transcriptional regulation enable maize (Zea mays), a crop originally domesticated in the tropics, to adapt to temperate environments.

Result: We generated 572 unique RNA-seq datasets from the roots of 340 maize genotypes. Genes involved in core processes such as cell division, chromosome organization and cytoskeleton organization showed lower heritability of gene expression, while genes involved in anti-oxidation activity exhibited higher expression heritability. An expression genome-wide association study (eGWAS) identified 19,602 expression quantitative trait loci (eQTLs) associated with the expression of 11,444 genes. A …

Recombination Hotspots In Soybean [Glycine Max (L.) Merr.], Samantha Mcconaughy, Keenan L. Amundsen, Qijian Song, Vince Pantalone, D. Hyten

Department of Agronomy and Horticulture: Faculty Publications

Recombination allows for the exchange of genetic material between two parents, which plant breeders exploit to make improved cultivars. This recombination is not distributed evenly across the chromosome. Recombination mostly occurs in euchromatic regions of the genome and even then, recombination is focused into clusters of crossovers termed recombination hotspots. Understanding the distribution of these hotspots along with the sequence motifs associated with them may lead to methods that enable breeders to better exploit recombination in breeding. To map recombination hotspots and identify sequence motifs associated with hotspots in soybean [Glycine max (L.) Merr.], two biparental recombinant inbred lines …

Large And Stable Genome Edits At The Sorghum Alpha Kafirin Locus Result In Changes In Chromatin Accessibility And Globally Increased Expression Of Genes Encoding Lysine Enrichment, J. Preston Hurst, Abou Yobi, Aixia Li, Shirley Sato, Thomas E. Clemente, Ruthie Angelovici

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sorghum is a resilient and widely cultivated grain crop used for feed and food. However, it’s grain is deficient in lysine, an essential amino acid. This is due to the primary seed storage proteins, the alpha-kafirins, lacking lysine. It has been observed that reductions in alpha-kafirin protein results in rebalancing of the seed proteome and a corresponding increase in non-kafirin proteins which leads to an increased lysine content. However, the mechanisms underlying proteome rebalancing are unclear. This study characterizes a previously developed gene edited sorghum line, with deletions at the alpha kafirin locus.

Methods: A single consensus …

Large And Stable Genome Edits At The Sorghum Alpha Kafirin Locus Result In Changes In Chromatin Accessibility And Globally Increased Expression Of Genes Encoding Lysine Enrichment, J. Preston Hurst, Abou Yobi, Aixia Li, Shirley Sato, Thomas E. Clemente, Ruthie Angelovici, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sorghum is a resilient and widely cultivated grain crop used for feed and food. However, it’s grain is deficient in lysine, an essential amino acid. This is due to the primary seed storage proteins, the alpha-kafirins, lacking lysine. It has been observed that reductions in alpha-kafirin protein results in rebalancing of the seed proteome and a corresponding increase in non-kafirin proteins which leads to an increased lysine content. However, the mechanisms underlying proteome rebalancing are unclear. This study characterizes a previously developed gene edited sorghum line, with deletions at the alpha kafirin locus.

Methods: A single consensus …

Intensifying A Crop–Fallow System: Impacts On Soil Properties, Crop Yields, And Economics, S. J. Ruis, S. Stepanovic, Humberto Blanco-Canqui

Department of Agronomy and Horticulture: Faculty Publications

Intensifying crop–fallow systems could address increased weed control costs, increased land or rental costs, reduced crop diversity, and degraded soil properties in water-limited environments. One strategy to intensify such systems could be the insertion of a short-season crop during fallow. But, how this strategy affects soils, crop production, and farm economics needs further research. Thus, we studied the impacts of replacing fallow in a winter wheat (Triticum aestivum L)–corn (Zea mays L.)–fallow system with a short-season spring crop [field pea (Pisum sativum L.)] on crop yields and economics from 2015 to 2019 and 5-yr cumulative effects on …

Abnormal Ear Development In Corn: Does Hybrid, Environment, And Seeding Rate Matter?, Osler A. Ortez, Anthony J. Mcmechan, Emily Robinson, Thomas C. Hoegemeyer, Reka Howard, Roger W. Elmore

Department of Agronomy and Horticulture: Faculty Publications

Corn (Zea mays L.) yields have increased in the United States since the 1930s and in other parts of the world since the 1950s and 1960s because of improvements in agricultural management and genotypes. Despite these increases, production concerns still exist. In July 2016, abnormal ear development (multi-ears per node, barbell-ears, and short-husks) was reported in cornfields that extended from the Texas Panhandle to eastern Colorado and east through Kansas, Nebraska, Iowa, and Illinois. Surveys in Nebraska farmer fields revealed significant productivity losses due to the issues, but little was known about the underlying causes. A research study was …