Plant Biology Commons^™

Long-Term Winter Wheat (Triticum Aestivum L.) Seasonal Irrigation Amount, Evapotranspiration, Yield, And Water Productivity Under Semiarid Climate, Koffi Djaman, Michael O'Neill, Curtis K. Owen, Daniel Smeal, Margaret West, Dallen Begay, Samuel Allen, Komlan Koudahe, Suat Irmak, Kevin Lombard

Department of Agronomy and Horticulture: Faculty Publications

A long-term field experiment was conducted from 2002 to 2014 for the evaluation of yield and water productivity of three winter wheat varieties—Kharkof, Scout 66, and TAM107—under sprinkler irrigation at New Mexico State University Agricultural Science Center at Farmington, NM. Winter wheat daily evapotranspiration was estimated following the United Nations Food and Agriculture Organization FAO crop coefficient approach (ETc = Kc ETo), and crop water use efficiency (CWUE), evapotranspiration water use efficiency (ETWUE), and irrigation water use efficiency (IWUE) were estimated for each growing season. There was inter-annual variation in seasonal precipitation and irrigation amounts. Seasonal irrigation amounts varied from …

Engineering Linear, Branched-Chain Triterpene Metabolism In Monocots, Chase Kempinski, Zuodong Jiang, Garrett Zinck, Shirley J. Sato, Zhengxiang Ge, Thomas E. Clemente, Joe Chappell

Department of Agronomy and Horticulture: Faculty Publications

Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to yield farnesyl diphosphate (FPP), catalyzed by FPP synthase (FPS). Squalene synthase (SQS) condenses two molecules of FPP to generate the symmetrical product squalene, the first committed precursor to sterols and most other triterpenes. In the green algae Botryococcus braunii, two FPP molecules can also be condensed in an asymmetric manner yielding the more highly branched triterpene, botryococcene. Botryococcene …

Genetic Architecture Of Common Bunt Resistance In Winter Wheat Using Genome-Wide Association Study, Amira M. I. Mourad, Ahmed Sallam, Vikas Belamkar, Ezzat Mahdy, Bahy Bakheit, Atif Abo El-Wafaa, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Background: Common bunt (caused by Tilletia caries and T. foetida) has been considered as a major disease in wheat (Triticum aestivum) following rust (Puccinia spp.) in the Near East and is economically important in the Great Plains, USA. Despite the fact that it can be easily controlled using seed treatment with fungicides, fungicides often cannot or may not be used in organic and low-input fields. Planting common bunt resistant genotypes is an alternative.

Results: To identify resistance genes for Nebraska common bunt race, the global set of differential lines were inoculated. Nine differential lines carrying nine …

Cc-Type Glutaredoxins Mediate Plant Response And Signaling Under Nitrate Starvation In Arabidopsis, Ji-Yul Jung, Ji Hoon Ahn, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Background: Nitrogen is an essential nutrient in plants. Despite the importance of nitrogen for plant growth and agricultural productivity, signal transduction pathways in response to nitrate starvation have not been fully elucidated in plants.

Results: Gene expression analysis and ectopic expression were used to discover that many CC-type glutaredoxins (ROXYs) are differentially expressed in response to nitrate deprivation. A gain-of-function approach showed that ROXYs may play a role in nutrient sensing through the regulation of chlorophyll content, root hair growth, and transcription of nitrate-related genes such as NRT2.1 under low or high nitrate conditions. Reactive oxygen species (ROS) …

Risks And Opportunities Of Gm Crops: Bt Maize Example, Andrea K. Carzoli, Siddique I. Aboobucker, Leah L. Sandall, Thomas T. Lubberstedt, Walter P. Suza

Department of Agronomy and Horticulture: Faculty Publications

The anticipated world population growth emphasizes a need to produce more food on less land. Cutting-edge technologies, including genetic engineering, can help to develop improved crop varieties and protect natural resources. In spite of the potential for genetically-modified (GM) crops to make crop production more efficient, they remain a polarizing issue due to safety concerns. This paper provides an overview of the risk assessment process. The safety of Bacillus thuringiensis (Bt) proteins is used as an example for how risk assessment is applied to GM crops. Risks associated with GM crops have proven to be low to non-existent. Developing countries …

Root And Shoot Contribution To Carbon And Nitrogen Inputs In The Topsoil Layer In No-Tillage Crop Systems Under Subtropical Conditions, Marciel Redin, Sylvie Recous, Celso Aita, Bruno Chaves, Ismael Cristiano Pfeifer, Leonardo Mendes Bastos, Getulio Elias Pilecco, Sandro Jose Giacomini

Department of Agronomy and Horticulture: Faculty Publications

Recycling of carbon (C) and nitrogen (N) from plants into soils is decisive for maintaining soil organic matter and soil fertility. Therefore, we quantified plant biomass and C and N in the shoots and roots from the topsoil layer for a wide range of annual crops grown under subtropical conditions. We grew 26 species, 13 main crops, and 13 cover crops, in the field in standard sowing arrangements. Root biomass was recovered from the 0.00-0.20 m soil layer at flowering, and shoot biomass was measured at flowering for all crops and at maturity only for the main crops. Root dry …

Genotype Imputation In Winter Wheat Using First-Generation Haplotype Map Snps Improves Genome-Wide Association Mapping And Genomic Prediction Of Traits, Moses Nyine, Shichen Wang, Kian Kiani, Katherine Jordan, Shuyu Liu, Patrick Byrne, Scott Haley, Stephen Baenziger, Shiaoman Chao, Robert Bowden, Eduard Akhunov

Department of Agronomy and Horticulture: Faculty Publications

Genome-wide single nucleotide polymorphism (SNP) variation allows for the capture of haplotype structure in populations and prediction of unobserved genotypes based on inferred regions of identity-by-descent (IBD). Here we have used a first-generation wheat haplotype map created by targeted re-sequencing of low-copy genomic regions in the reference panel of 62 lines to impute marker genotypes in a diverse panel of winter wheat cultivars from the U.S. Great Plains. The IBD segments between the reference population and winter wheat cultivars were identified based on SNP genotyped using the 90K iSelect wheat array and genotyping by sequencing (GBS). A genome-wide association study …

Genome-Wide Association Study Reveals Novel Genomic Regions Associated With 10 Grain Minerals In Synthetic Hexaploid Wheat, Madhav Bhatta, P. Stephen Baenziger, Brian M. Waters, Rachana Poudel, Vikas Belamkar, Jesse Poland, Alexey Morgounov

Department of Agronomy and Horticulture: Faculty Publications

Synthetic hexaploid wheat (SHW; Triticum durum L. x Aegilops tauschii Coss.) is a means of introducing novel genes/genomic regions into bread wheat (T. aestivum L.) and a potential genetic resource for improving grain mineral concentrations. We quantified 10 grain minerals (Ca, Cd, Cu, Co, Fe, Li, Mg, Mn, Ni, and Zn) using an inductively coupled mass spectrometer in 123 SHWs for a genome-wide association study (GWAS). A GWAS with 35,648 single nucleotide polymorphism (SNP) markers identified 92 marker-trait associations (MTAs), of which 60 were novel and 40 were within genes, and the genes underlying 20 MTAs had annotations suggesting …

Stolon Development And Cultural Production Practices Of Winter-Grown Strawberries, Donna M. Morrison, Erin Blankenship, Paul Read, Ellen Paparozzi

Department of Agronomy and Horticulture: Faculty Publications

The four experiments described here are part of a 5-year program focused on determining whether day-neutral and short-day strawberries would be suitable for commercial offseason/ winter (temperate climate) production in greenhouses. All research was performed using container-grown plants fertigated through a capillary mat production system with heat supplied under the benches. The first experiment focused on stolon development. The second experiment investigated the influence of stolon removal on berry production and included the removal of flowers for the first three weeks. The third and fourth experiments were conducted as a comparison of university research (experiment 3) versus a commercial operation …

Wildland Fire Science Literacy: Education, Creation, And Application, Devan Allen Mcgranahan, Carissa L. Wonkka

Department of Agronomy and Horticulture: Faculty Publications

Wildland fire science literacy is the capacity for wildland fire professionals to understand and communicate three aspects of wildland fire: (1) the fundamentals of fuels and fire behavior, (2) the concept of fire as an ecological regime, and (3) multiple human dimensions of wildland fire and the socio-ecological elements of fire regimes. Critical to wildland fire science literacy is a robust body of research on wildland fire. Here, we describe how practitioners, researchers, and other professionals can study, create, and apply robust wildland fire science. We begin with learning and suggest that the conventional fire ecology canon include detail on …

An Exome-Seq Based Tool For Mapping And Selection Of Candidate Genes In Maize Deletion Mutants, Shangang Jia, Kyla Morton, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Despite the large number of genomic and transcriptomic resources in maize, there is still much to learn about the function of genes in developmental and biochemical processes. Some maize mutants that were generated by gamma-irradiation showed clear segregation for the kernel phenotypes in B73 X Mo17 F2 ears. To better understand the functional genomics of kernel development, we developed a mapping and gene identification pipeline, bulked segregant exome sequencing (BSEx-seq), to map mutants with kernel phenotypes including opaque endosperm and reduced kernel size. BSEx-seq generates and compares the sequence of the exon fraction from mutant and normal plant F2 DNA …

Genetic Architecture Of Soybean Yield And Agronomic Traits, Brian W. Diers, Jim Specht, Katy Martin Rainey, Perry Cregan, Qijian Song, Vishnu Ramasubramanian, George Graef, Randall L. Nelson, William Schapaugh, Dechun Wang, Grover Shannon, Leah Mchale, Stella K. Kantartzi, Alencar Xavier, Rouf Mian, Robert M. Stupar, Jean-Michel Michno, Yong-Qiang Charles An, Wolfgang Goettel, Russell Ward, Carolyn Fox, Alexander E. Lipka, David Hyten, Troy Cary, William D. Beavis

Department of Agronomy and Horticulture: Faculty Publications

Soybean is the world’s leading source of vegetable protein and demand for its seed continues to grow. Breeders have successfully increased soybean yield, but the genetic architecture of yield and key agronomic traits is poorly understood. We developed a 40-mating soybean nested association mapping (NAM) population of 5,600 inbred lines that were characterized by single nucleotide polymorphism (SNP) markers and six agronomic traits in field trials in 22 environments. Analysis of the yield, agronomic, and SNP data revealed 23 significant marker-trait associations for yield, 19 for maturity, 15 for plant height, 17 for plant lodging, and 29 for seed mass. …

Influence Of Harvest Date On Seed Yield And Quality In Forage Kochia, Cody F. Creech, Blair L. Waldron, Corey V. Ransom, Dale R. Zobell, Joseph Earl Creech

Department of Agronomy and Horticulture: Faculty Publications

Forage kochia (Bassia prostrata) is used for rangeland reclamation and livestock and wildlife forage, but limited research has been conducted on its seed production. Therefore, this research evaluated the effect of harvest date on seed weight, germination, and seed yield of forage kochia subspecies virescens and grisea. Seed was harvested from individual plants for 3 years during October, November, and December. October harvest had the lightest 100-seed weights, with the November harvest slightly heavier than December, for most accessions. Cultivar Snowstorm and breeding line Sahsel, both subsp. grisea, had the greatest 100-seed weights in November, 155 …

Transient Heat Stress During Early Seed Development Primes Germination And Seedling Establishment In Rice, Kevin Begcy, Jaspreet Sandhu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Rice yield is highly sensitive to increased temperature. Given the trend of increasing global temperatures, this sensitivity to higher temperatures poses a challenge for achieving global food security. Early seed development in rice is highly sensitive to unfavorable environmental conditions. Heat stress (HS) during this stage decreases seed size and fertility, thus reducing yield. Here, we explore the transgenerational phenotypic consequences of HS during early seed development on seed viability, germination, and establishment. To elucidate the impact of HS on the developmental events in postzygotic rice seeds, we imposed moderate (35°C) and severe (39°C) HS treatments initiated 1 day after …

Maize And Soybean Root Front Velocity And Maximum Depth In Iowa, Usa, Raziel A. Ordóñez, Michael J. Castellano, J. L. Hatfield, M. J. Helmers, Mark A. Licht, Matt Liebman, Ranae Dietzel, Rafael Martinez-Feria, Javed Iqbal, Laila A. Puntel, S. Carolina Córdova, Kaitlin Togliatti, Emily E. Wright, Sotirios V. Archontoulis

Department of Agronomy and Horticulture: Faculty Publications

Quantitative measurements of root traits can improve our understanding of how crops respond to soil and weather conditions, but such data are rare. Our objective was to quantify maximum root depth and root front velocity (RFV) for maize (Zea mays) and soybean (Glycine max) crops across a range of growing conditions in the Midwest USA. Two sets of root measurements were taken every 10–15 days: in the crop row (in-row) and between two crop rows (center-row) across six Iowa sites having different management practices such as planting dates and drainage systems, totaling 20 replicated experimental treatments. …

Sequences Involved In Plant Yield And Methods Of Using, Harkamal Walia, Dante Placido, Thomas E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Nucleic acid sequences involved in plant yield are provided, as are methods of using such nucleic acid sequences .

Identification Of Quantitative Trait Loci Conferring Resistance To Tan Spot In A Biparental Population Derived From Two Nebraska Hard Red Winter Wheat Cultivars, Gayan K. Kariyawasam, Waseem Hussain, Amanda Easterly, Mary Guttieri, Vikas Belamkar, Jesse Poland, Jorge Venegas, Stephen Baenziger, Francois Marais, Jack B. Rasmussen, Zhaohui Liu

Department of Agronomy and Horticulture: Faculty Publications

Tan spot, caused by Pyrenophora triticirepentis (Ptr), is a destructive foliar disease in all types of cultivated wheat worldwide. Genetics of tan spot resistance in wheat is complex, involving insensitivity to fungal-produced necrotrophic effectors (NEs), major resistance genes, and quantitative trait loci (QTL) conferring race-nonspecific and race-specific resistance. The Nebraska hard red winter wheat (HRWW) cultivar ‘Wesley’ is insensitive to Ptr ToxA and highly resistant to multiple Ptr races, but the genetics of resistance in this cultivar is unknown. In this study, we used a recombinant inbred line (RIL) population derived from a cross between Wesley and another …

Active-Optical Reflectance Sensing Corn Algorithms Evaluated Over The United States Midwest Corn Belt, G. M. Bean, N. R. Kitchen, J. J. Camberato, R. B. Ferguson, F. G. Fernandez, D. W. Franzen, C. A. M. Laboski, E. D. Nafziger, J. E. Sawyer, P. C. Scharf, J. Schepers, J. S. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Uncertainty exists with corn (Zea mays L.) N management due to year-to-year variation in crop N need, soil N supply, and N loss from leaching, volatilization, and denitrification. Activeoptical reflectance sensing (AORS) has proven effective in some fields for generating N fertilizer recommendations that improve N use efficiency, but locally derived (e.g., within a US state) AORS algorithms have not been tested simultaneously across a broad region. The objective of this research was to evaluate locally developed AORS algorithms across the US Midwest Corn Belt region for making in-season corn N recommendations. Forty-nine N response trials were conducted across …

Substantial Contribution Of Genetic Variation In The Expression Of Transcription Factors To Phenotypic Variation Revealed By Erd-Gwas, Hung-Ying Lin, Qiang Liu, Xiao Li, Jinliang Yang, Sanzhen Liu, Yinlian Huang, Michael J. Scanlon, Dan Nettleton, Patrick S. Schnable

Department of Agronomy and Horticulture: Faculty Publications

Background: There are significant limitations in existing methods for the genome-wide identification of genes whose expression patterns affect traits.

Results: The transcriptomes of five tissues from 27 genetically diverse maize inbred lines were deeply sequenced to identify genes exhibiting high and low levels of expression variation across tissues or genotypes. Transcription factors are enriched among genes with the most variation in expression across tissues, as well as among genes with higher-than-median levels of variation in expression across genotypes. In contrast, transcription factors are depleted among genes whose expression is either highly stable or highly variable across genotypes. We developed a …

Estimation Of Irrigation Requirements For Drip-Irrigated Maize In A Sub-Humid Climate, Liu Yang, Hai-Shun Yang, Jiu-Sheng Li, Yan-Feng Li, Hai-Jun Yan

Department of Agronomy and Horticulture: Faculty Publications

Drip-irrigation is increasingly applied in maize (Zea mays L.) production in sub-humid region. It is critical to quantify irrigation requirements during different growth stages under diverse climatic conditions. In this study, the Hybrid-Maize model was calibrated and applied in a sub-humid Heilongjiang Province in Northeast China to estimate irrigation requirements for drip irrigated maize during different crop physiological development stages and under diverse agro-climatic conditions. Using dimensionless scales, the whole growing season of maize was divided into diverse development stages from planting to maturity. Drip-irrigation dates and irrigation amounts in each irrigation event were simulated and summarized in 30-year simulation …

Identifying Ecologically Relevant Scales Of Habitat Selection: Diel Habitat Selection In Elk, Caleb P. Roberts, James W. Cain Iii, Robert D. Cox

Department of Agronomy and Horticulture: Faculty Publications

Although organisms make resource selection decisions at multiple spatiotemporal scales, not all scales are ecologically relevant to any given organism. Ecological patterns and rhythms such as behavioral and climatic patterns may provide a consistent method for identifying ecologically relevant scales of habitat selection. Using elk (Cervus canadensis) as an example species, we sought to test the ability of behavioral patterns to empirically partition diel scales for modeling habitat selection. We used model selection to partition diel scales by shifts in dominant behavior and then used resource selection probability functions to model elk habitat selection hierarchically at diel scales within seasons. …

Effects Of Nitrogen And Phosphorus Fertilizer And Topsoil Amendment On Native Plant Cover In Roadside Revegetation Projects, Heidi L. Hillhouse, Walter H. Schacht, Jonathan M. Soper, Carol E. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Establishing vegetation on roadsides following construction can be challenging, especially for relatively slow growing native species. Topsoil is generally removed during construction, and the surface soil following construction (“cut-slope soils”) is often compacted and low in nutrients, providing poor growing conditions for vegetation. Nebraska Department of Transportation (NDOT) protocols have historically called for nitrogen (N) and phosphorus (P) fertilization when planting roadside vegetation following construction, but these recommendations were developed for cool-season grass plantings and most current plantings use slower-establishing, native warmseason grasses that may benefit less than expected from current planting protocols. We evaluated the effects of nitrogen and …

Recognizing Challenges And Opportunities In The Quest To End Hunger, Jennifer Williams Zwagerman

Texas A&M Law Review

As an attorney and professor that does not focus on intellectual property law, I was a bit apprehensive about providing a keynote address for a Symposium focusing on “Agriculture, Intellectual Property, and Feeding the World in the 21st Century.” As I thought about this topic, knowing that there were other speakers who would focus more on the IP issues and technical aspects of various topics, I kept coming back to the importance of technology as we worktowards the goal of feeding the world, and the many ways in which innovation plays a role in meeting that goal. It also brought …

Control Of Glyphosate-Resistant Common Ragweed (Ambrosia Artemisiifolia L.) In Glufosinate-Resistant Soybean [Glycine Max (L.) Merr], Ethann R. Barnes, Stevan Z. Knezevic, Peter H. Sikkema, John L. Lindquist, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Common ragweed emerges early in the season in Nebraska, USA and is competitive with soybean; therefore, preplant herbicides are important for effective control. Glyphosate has been used as a preplant control option; however, confirmation of glyphosate-resistant (GR) common ragweed in Nebraska necessitates evaluating other herbicide options. The objectives of this study were to (1) evaluate the efficacy of preplant (PP) herbicides followed by (fb) glufosinate alone or in tank-mixture with imazethapyr, acetochlor, or S-metolachlor applied post-emergence (POST) for control of GR common ragweed in glufosinate-resistant soybean; (2) their effect on common ragweed density, biomass, and soybean yield; and (3) the …

Impact Of Seed Protein Alleles From Three Soybean Sources On Seed Composition And Agronomic Traits, Lillian F. Brzostowski, Timothy I. Pruski, James Specht, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Evaluation of seed protein alleles in soybean populations showed that an increase in protein concentration is generally associated with a decrease in oil concentration and yield.

Soybean [Glycine max (L.) Merrill] meal is one of the most important plant-based protein sources in the world. Developing cultivars high in seed protein concentration and seed yield is a difficult task because the traits have an inverse relationship. Over two decades ago, a protein quantitative trait loci (QTL) was mapped on chromosome (chr) 20, and this QTL has been mapped to the same position in several studies and given the confirmed QTL …

Molecular And Culture-Based Assessment Of Root/Crown Rot Fungal/Oomycete Complex Of Bean In Mozambique, Suzana Vanessa Ismael Fernandes

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Dry edible beans (Phaseolus vulgaris) play an important role in food security, alleviating hunger and malnutrition and adding value to the economy in Mozambique. Recently, root/crown rot (RCR) has caused yield losses in bean fields in Mozambique. A diverse taxa of fungi and oomycetes have been associated with RCR. However, in Mozambique little information on root rot is available. The purpose of this study is to use Illumina sequencing platform, polymerase chain reaction (PCR) and culture-based methods to identify the primary pathogens associated with RCR in Mozambique. A total of 88 plants showing symptoms of RCR disease were …

Inferring Foliar Water Uptake Using Stable Isotopes Of Water, Gregory R. Goldsmith, Marco M. Lehmann, Lucas A. Cernusak, Matthias Arend, Rolf T.W. Siegwolf

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

A growing number of studies have described the direct absorption of water into leaves, a phenomenon known as foliar water uptake. The resultant increase in the amount of water in the leaf can be important for plant function. Exposing leaves to isotopically enriched or depleted water sources has become a common method for establishing whether or not a plant is capable of carrying out foliar water uptake. However, a careful inspection of our understanding of the fluxes of water isotopes between leaves and the atmosphere under high humidity conditions shows that there can clearly be isotopic exchange between the two …

Cellular And Molecular Targets Of Menthol Actions, Murat Oz, Eslam El Nebrisi, Keun-Hang Susan Yang, Frank Christopher Howarth, Lina T. Al Kury

Mathematics, Physics, and Computer Science Faculty Articles and Research

Menthol belongs to monoterpene class of a structurally diverse group of phytochemicals found in plant-derived essential oils. Menthol is widely used in pharmaceuticals, confectionary, oral hygiene products, pesticides, cosmetics, and as a flavoring agent. In addition, menthol is known to have antioxidant, anti-inflammatory, and analgesic effects. Recently, there has been renewed awareness in comprehending the biological and pharmacological effects of menthol. TRP channels have been demonstrated to mediate the cooling actions ofmenthol. There has been new evidence demonstrating thatmenthol can significantly influence the functional characteristics of a number of different kinds of ligand and voltage-gated ion channels, indicating that at …

Hydrogenation Of Organic Matter As A Terminal Electron Sink Sustains High Co2:Ch4 Production Ratios During Anaerobic Decomposition, Rachel M. Wilson, Malak M. Tfaily, Virginia I. Rich, Jason K. Keller, Scott D. Bridgham, Cassandra Medvedeff Zalman, Laura Meredith, Paul J. Hanson, Mark Hines, Laurel Pfeifer-Meister, Scott R. Saleska, Patrick Crill, William T. Cooper, Jeff P. Chanton, Joel E. Kostka

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Once inorganic electron acceptors are depleted, organic matter in anoxic environments decomposes by hydrolysis, fermentation, and methanogenesis, requiring syntrophic interactions between microorganisms to achieve energetic favorability. In this classic anaerobic food chain, methanogenesis represents the terminal electron accepting (TEA) process, ultimately producing equimolar CO₂ and CH₄ for each molecule of organic matter degraded. However, CO₂:CH₄ production in Sphagnum-derived, mineral-poor, cellulosic peat often substantially exceeds this 1:1 ratio, even in the absence of measureable inorganic TEAs. Since the oxidation state of C in both cellulose-derived organic matter and acetate is 0, and CO₂ has …

Protocol For Enrichment Of The Membrane Proteome Of Mature Tomato Pollen, Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff

Department of Agronomy and Horticulture: Faculty Publications

We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.