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Full-Text Articles in Plant Biology

Rekayasa Source – Sink Dengan Pemberian Zat Pengatur Tumbuh Untuk Meningkatkan Produksi Benih Kentang Di Dataran Medium Desa Margawati Kabupaten Garut, Anne Nuraini, Yayat Rochayat Suradinata, Dedi Widayat Mar 2016

Rekayasa Source – Sink Dengan Pemberian Zat Pengatur Tumbuh Untuk Meningkatkan Produksi Benih Kentang Di Dataran Medium Desa Margawati Kabupaten Garut, Anne Nuraini, Yayat Rochayat Suradinata, Dedi Widayat

Jurnal Kultivasi

Produksi kentang dipengaruhi oleh keter-sediaan benihnya. Kurangnya pasokan atau ketersediaan benih kentang akan berpengaruh pada produksi kentang. Penelitian ini dilakukan untuk menguji kualitas ubi kentang G2 yang dihasilkan dengan sistem Nutrient Film Technique (NFT) di lapangan. Percobaan dilakukan pada dataran medium di Garut dengan ketinggian tempat sekitar 700 m di atas permukaan laut untuk menghasilkan benih kentang G3 dengan perlakuan aplikasi sitokinin 0, 5, 10 dan 15 ml/L dan paklobutrazol 0, 15, 30 dan 45 ml/L. Percobaan menggunakan Rancangan Acak Kelompok Pola Faktorial. Faktor pertama adalah konsentrasi sitokinin yaitu: 0, 5, 10 dan 15 ml/L, dan faktor kedua adalah konsentrasi …


Information Thermodynamics Of Cytosine Dna Methylation, Robersy Sanchez Rodriguez, Sally Ann Mackenzie Mar 2016

Information Thermodynamics Of Cytosine Dna Methylation, Robersy Sanchez Rodriguez, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Cytosine DNA methylation (CDM) is a stable epigenetic modification to the genome and a widespread regulatory process in living organisms that involves multicomponent molecular machines. Genome-wide cytosine methylation patterning participates in the epigenetic reprogramming of a cell, suggesting that the biological information contained within methylation positions may be amenable to decoding. Adaptation to a new cellular or organismal environment also implies the potential for genome-wide redistribution of CDM changes that will ensure the stability of DNA molecules. This raises the question of whether or not we would be able to sort out the regulatory methylation signals from the CDM background …


Ws-2 Introgression In A Proportion Of Arabidopsis Thaliana Col-0 Stock Seed Produces Specific Phenotypes And Highlights The Importance Of Routine Genetic Verification, Mon-Ray Shao, Vikas Shedge, Hardik Kundariya, Fredric R. Lehle, Sally Ann Mackenzie Mar 2016

Ws-2 Introgression In A Proportion Of Arabidopsis Thaliana Col-0 Stock Seed Produces Specific Phenotypes And Highlights The Importance Of Routine Genetic Verification, Mon-Ray Shao, Vikas Shedge, Hardik Kundariya, Fredric R. Lehle, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Arabidopsis thaliana is an important model organism with a robust network of resources that has been of enormous value to the plant science research community. The use of isogenic material as a reference point or control is critical for many types of experiments in plant molecular biology and genetics. Recently, we noticed that some seed from a common source of the widely used Columbia-0 (Col-0) strain gave rise to plants showing features atypical for this strain. Whole-genome DNA-sequencing and allelespecific PCR assays confirmed that the abnormal individuals contain multiple introgressions from the ecotype Wassilewskija-2 (Ws-2), as described below. This …


Student-Conducted Farmer Video Interviews, Jenn K. Simons, Justin Van Wart, Charles Francis, Kristyn Harms Mar 2016

Student-Conducted Farmer Video Interviews, Jenn K. Simons, Justin Van Wart, Charles Francis, Kristyn Harms

Department of Agronomy and Horticulture: Faculty Publications

High school agricultural education teachers have expressed concern about the lack of easily accessible educational materials dealing with contemporary topics in sustainable agriculture. There are numerous textbooks and monographs available for farmers and students at the college level, including the highly practical resources available from the Sustainable Agriculture Research and Education (SARE) book series on soil fertility (Magdoff and van Es, 2010), cover crops (Bowman et al., 2007) and building a farm business (DiGiacomo et al., 2003), among others. Although these are full of color photos and easily accessible graphs and tables, they are still in the print media category. …


Corn Era Hybrid Response To Nitrogen Fertilization, Krishna P. Woli, Matthew J. Boyer, Roger Wesley Elmore, John E. Sawyer, Lori J. Abendroth, Daniel W. Barker Jan 2016

Corn Era Hybrid Response To Nitrogen Fertilization, Krishna P. Woli, Matthew J. Boyer, Roger Wesley Elmore, John E. Sawyer, Lori J. Abendroth, Daniel W. Barker

Department of Agronomy and Horticulture: Faculty Publications

Corn (Zea mays L.) N use is of continued interest due to agronomic performance and environmental issues. This 2-yr study evaluated era hybrid response to fertilizer nitrogen (FN) rate in a factorial arrangement of one popular hybrid per five decades (1960–2000 eras) and five N rates (0–224 kg N ha–1). An additional hybrid per era was grown at 168 kg N ha–1. Hybrid productivity and nitrogen use efficiency (NUE) increased across the eras, but not between the 1980 and 1990 eras. Grain yield (GY) increased 65% and total plant biomass 43%, however, total plant nitrogen …


Decipher The Molecular Response Of Plant Single Cell Types To Environmental Stresses, Mehrnoush Nourbakhsh-Rey, Marc Libault Jan 2016

Decipher The Molecular Response Of Plant Single Cell Types To Environmental Stresses, Mehrnoush Nourbakhsh-Rey, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

The analysis of the molecular response of entire plants or organs to environmental stresses suffers from the cellular complexity of the samples used. Specifically, this cellular complexity masks cell-specific responses to environmental stresses and logically leads to the dilution of the molecular changes occurring in each cell type composing the tissue/organ/plant in response to the stress.Therefore, to generate a more accurate picture of these responses, scientists are focusing on plant single cell type approaches. Several cell types are now considered as models such as the pollen, the trichomes, the cotton fiber, various root cell types including the root hair cell, …


Draft Genome Sequence Of Erwinia Tracheiphila, An Economically Important Bacterial Pathogen Of Cucurbits, Lori R. Shapiro, Erin D. Scully, Dana Roberts, Timothy J. Straub, Scott M. Geib, Jihye Park, Andrew G. Stephenson, Erika Salaau Rojas, Quin Liu, Gwyn Beattie, Mark Gleason, Consuelo M. De Moraes, Mark C. Mescher, Shelby G. Fleischer, Roberto Kolter, Naomi Pierce, Olga Zhaxybayeva Jan 2016

Draft Genome Sequence Of Erwinia Tracheiphila, An Economically Important Bacterial Pathogen Of Cucurbits, Lori R. Shapiro, Erin D. Scully, Dana Roberts, Timothy J. Straub, Scott M. Geib, Jihye Park, Andrew G. Stephenson, Erika Salaau Rojas, Quin Liu, Gwyn Beattie, Mark Gleason, Consuelo M. De Moraes, Mark C. Mescher, Shelby G. Fleischer, Roberto Kolter, Naomi Pierce, Olga Zhaxybayeva

Department of Agronomy and Horticulture: Faculty Publications

Erwinia tracheiphila is one of the most economically important pathogens of cucumbers, melons, squashes, pumpkins, and gourds in the northeastern and midwestern United States, yet its molecular pathology remains uninvestigated. Here, we report the first draft genome sequence of an E. tracheiphila strain isolated from an infected wild gourd (Cucurbita pepo subsp. texana) plant. The genome assembly consists of 7 contigs and includes a putative plasmid and at least 20 phage and prophage elements.


Genome-Wide Discriminatory Information Patterns Of Cytosine Dna Methylation, Robersy Sanchez, Sally A. Mackenzie Jan 2016

Genome-Wide Discriminatory Information Patterns Of Cytosine Dna Methylation, Robersy Sanchez, Sally A. Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Cytosine DNA methylation (CDM) is a highly abundant, heritable but reversible chemical modification to the genome. Herein, a machine learning approach was applied to analyze the accumulation of epigenetic marks in methylomes of 152 ecotypes and 85 silencing mutants of Arabidopsis thaliana. In an information-thermodynamics framework, two measurements were used: (1) the amount of information gained/lost with the CDM changes IR and (2) the uncertainty of not observing a SNP LCR. We hypothesize that epigenetic marks are chromosomal footprints accounting for different ontogenetic and phylogenetic histories of individual populations. A machine learning approach is proposed to …


Molecular Characterization Of Resistance To Soybean Rust (Phakopsora Pachyrhizi Syd. & Syd.) In Soybean Cultivar Dt 2000 (Pi 635999), Tri D. Vuong, David R. Walker, Binh T. Nguyen, Tuyet T. Nguyen, Hoan X. Dinh, David L. Hyten, Perry B. Cregan, David A. Sleper, Jeong D. Lee, James G. Shannon, Henry T. Nguyen Jan 2016

Molecular Characterization Of Resistance To Soybean Rust (Phakopsora Pachyrhizi Syd. & Syd.) In Soybean Cultivar Dt 2000 (Pi 635999), Tri D. Vuong, David R. Walker, Binh T. Nguyen, Tuyet T. Nguyen, Hoan X. Dinh, David L. Hyten, Perry B. Cregan, David A. Sleper, Jeong D. Lee, James G. Shannon, Henry T. Nguyen

Department of Agronomy and Horticulture: Faculty Publications

Resistance to soybean rust (SBR), caused by Phakopsora pachyrhizi Syd. & Syd., has been identified in many soybean germplasm accessions and is conferred by either dominant or recessive genes that have been mapped to six independent loci (Rpp1 -Rpp6), but No U. S. cultivars are resistant to SBR. The cultivar DT 2000 (PI 635999) has resistance to P. pachyrhizi isolates and field populations from the United States as well as Vietnam. A F6:7 recombinant inbred line (RIL) population derived from Williams 82 × DT 2000 was used to identify genomic regions associated with resistance to SBR in …


Genome-Wide Association Mapping Of Qualitatively Inherited Traits In A Germplasm Collection, Nonoy B. Bandillo, Aaron J. Lorenz, George L. Graef, Diego Jarquin, David L. Hyten, Randall L. Nelson, James E. Specht Jan 2016

Genome-Wide Association Mapping Of Qualitatively Inherited Traits In A Germplasm Collection, Nonoy B. Bandillo, Aaron J. Lorenz, George L. Graef, Diego Jarquin, David L. Hyten, Randall L. Nelson, James E. Specht

Department of Agronomy and Horticulture: Faculty Publications

Genome-wide association (GWA) has been used as a tool for dissecting the genetic architecture of quantitatively inherited traits. We demonstrate here that GWA can also be highly useful for detecting many major genes governing categorically defined phenotype variants that exist for qualitatively inherited traits in a germplasm collection. Genome-wide association mapping was applied to categorical phenotypic data available for 10 descriptive traits in a collection of ~13,000 soybean [Glycine max (L.) Merr.] accessions that had been genotyped with a 50,000 single nucleotide polymorphism (SNP) chip. A GWA on a panel of accessions of this magnitude can offer substantial statistical …


Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding Jan 2016

Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such …


Modeling Shattercane Dynamics In Herbicide-Tolerant Grain Sorghum Cropping Systems, Rodrigo Werle, Brigitte Tenhumberg, John L. Lindquist Jan 2016

Modeling Shattercane Dynamics In Herbicide-Tolerant Grain Sorghum Cropping Systems, Rodrigo Werle, Brigitte Tenhumberg, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Traditional breeding technology is currently being used to develop grain sorghum [Sorghum bicolor (L.) Moench ssp. bicolor] germplasm that will be tolerant to acetolactate synthase (ALS)-inhibiting herbicides. This technology (InzenTM, DuPontTM) has the potential to improve sorghum production by allowing for the postemergence control of traditionally hard-to-control grasses. However, grain sorghum and shattercane [weedy Sorghum species; Sorghum bicolor (L.) Moench ssp. drummondii (Nees ex Steud.) de Wet ex Davidse] can interbreed and introduced traits such as herbicide tolerance could increase the weediness of the weedy relative. Our objective was to develop a simulation model …


Genomic Prediction Of Single Crosses In The Early Stages Of A Maize Hybrid Breeding Pipeline, Dnyaneshwar C. Kadam, Sarah M. Potts, Martin O. Bohn, Alexander E. Lipka, Aaron J. Lorenz Jan 2016

Genomic Prediction Of Single Crosses In The Early Stages Of A Maize Hybrid Breeding Pipeline, Dnyaneshwar C. Kadam, Sarah M. Potts, Martin O. Bohn, Alexander E. Lipka, Aaron J. Lorenz

Department of Agronomy and Horticulture: Faculty Publications

Prediction of single-cross performance has been a major goal of plant breeders since the beginning of hybrid breeding. Recently, genomic prediction has shown to be a promising approach, but only limited studies have examined the accuracy of predicting single-cross performance. Moreover, no studies have examined the potential of predicting single crosses among random inbreds derived from a series of biparental families, which resembles the structure of germplasm comprising the initial stages of a hybrid maize breeding pipeline. The main objectives of this study were to evaluate the potential of genomic prediction for identifying superior single crosses early in the hybrid …


Dioxygenase-Encoding Atdao1 Gene Controls Iaa Oxidation And Homeostasis In Arabidopsis, Silvana Porco, Aleš Pěnčík, Afaf Rashed, Ute Voβ, Rubén Casanova-Sáez, Anthony Bishop, Agata Golebiowska, Rahul Bhosale, Ranjan Swarup, Kamal Swarup, Pavlína Penáková, Ondrej Novák, Paul E. Staswick, Peter Hedden, Andrew L. Phillips, Kris Vissenberg, Malcolm J. Bennett, Karin Ljung Jan 2016

Dioxygenase-Encoding Atdao1 Gene Controls Iaa Oxidation And Homeostasis In Arabidopsis, Silvana Porco, Aleš Pěnčík, Afaf Rashed, Ute Voβ, Rubén Casanova-Sáez, Anthony Bishop, Agata Golebiowska, Rahul Bhosale, Ranjan Swarup, Kamal Swarup, Pavlína Penáková, Ondrej Novák, Paul E. Staswick, Peter Hedden, Andrew L. Phillips, Kris Vissenberg, Malcolm J. Bennett, Karin Ljung

Department of Agronomy and Horticulture: Faculty Publications

Auxin represents a key signal in plants, regulating almost every aspect of their growth and development. Major breakthroughs have been made dissecting the molecular basis of auxin transport, perception, and response. In contrast, how plants control the metabolism and homeostasis of the major form of auxin in plants, indole-3- acetic acid (IAA), remains unclear. In this paper, we initially describe the function of the Arabidopsis thaliana gene DIOXYGENASE FOR AUXIN OXIDATION 1 (AtDAO1). Transcriptional and translational reporter lines revealed that AtDAO1 encodes a highly root-expressed, cytoplasmically localized IAA oxidase. Stable isotope-labeled IAA feeding studies of loss and …


The Draft Genome Of The C3 Panicoid Grass Species Dichanthelium Oligosanthes, Anthony J. Studer, James C. Schnable, Sarit Weissmann, Allison R. Kolbe, Michael R. Mckain, Ying Shao, Asaph B. Cousins, Elizabeth A. Kellogg, Thomas P. Brutnell Jan 2016

The Draft Genome Of The C3 Panicoid Grass Species Dichanthelium Oligosanthes, Anthony J. Studer, James C. Schnable, Sarit Weissmann, Allison R. Kolbe, Michael R. Mckain, Ying Shao, Asaph B. Cousins, Elizabeth A. Kellogg, Thomas P. Brutnell

Department of Agronomy and Horticulture: Faculty Publications

Background: Comparisons between C3 and C4 grasses often utilize C3 species from the subfamilies Ehrhartoideae or Pooideae and C4 species from the subfamily Panicoideae, two clades that diverged over 50 million years ago. The divergence of the C3 panicoid grass Dichanthelium oligosanthes from the independent C4 lineages represented by Setaria viridis and Sorghum bicolor occurred approximately 15 million years ago, which is significantly more recent than members of the Bambusoideae, Ehrhartoideae, and Pooideae subfamilies. D. oligosanthes is ideally placed within the panicoid clade for comparative studies of C3 and C4 grasses.

Results: …


Estimating Yield Potential In Temperate High-Yielding, Direct-Seeded Us Rice Production Systems, Matthew B. Espe, Haishun Yang, Kenneth Cassman, Nicolas Guilpart, Hussain Sharifi, Bruce A. Linquist Jan 2016

Estimating Yield Potential In Temperate High-Yielding, Direct-Seeded Us Rice Production Systems, Matthew B. Espe, Haishun Yang, Kenneth Cassman, Nicolas Guilpart, Hussain Sharifi, Bruce A. Linquist

Department of Agronomy and Horticulture: Faculty Publications

Accurate estimation of a crop’s yield potential (Yp) is critical to addressing long-term food security via identification of the exploitable yield gap. Due to lack of field data, efforts to quantify crop yield potential typically rely on crop models. Using the ORYZA rice crop model, we sought to estimate Yp of irrigated rice for two widely used rice varieties (M-206 and CXL745) in three major US rice-producing regions that together represent some of the highest yielding rice regions of the world. Three major issues with the crop model had to be addressed to achieve acceptable simulation of Yp; first, the …


Yield Gap Analysis Of Us Rice Production Systems Shows Opportunities For Improvement, Matthew B. Espe, Kenneth Cassman, Haishun Yang, Nicolas Guilpart, Patricio Grassini, Justin Van Wart, Merle Anders, Donn Beighley, Dustin Harrell, Steve Linscombe, Kent Mckenzie, Randall Mutters, Lloyd T. Wilson, Bruce A. Linquist Jan 2016

Yield Gap Analysis Of Us Rice Production Systems Shows Opportunities For Improvement, Matthew B. Espe, Kenneth Cassman, Haishun Yang, Nicolas Guilpart, Patricio Grassini, Justin Van Wart, Merle Anders, Donn Beighley, Dustin Harrell, Steve Linscombe, Kent Mckenzie, Randall Mutters, Lloyd T. Wilson, Bruce A. Linquist

Department of Agronomy and Horticulture: Faculty Publications

Many assessments of crop yield gaps based on comparisons to actual yields suggest grain yields in highly intensified agricultural systems are at or near the maximum yield attainable. However, these estimates can be biased in situations where yields are below full yield potential. Rice yields in the US continue to increase annually, suggesting that rice yields are not near the potential. In the interest of directing future efforts towards areas where improvement is most easily achieved, we estimated yield potential and yield gaps in US rice production systems, which are amongst the highest yielding rice systems globally. Zones around fourteen …


Smokey Comes Of Age: Unmanned Aerial Systems For Fire Management, Dirac L. Twidwell Jr., Craig R. Allen, Carrick Detweiler, James Higgins, Christian Laney, Sebastian Elbaum Jan 2016

Smokey Comes Of Age: Unmanned Aerial Systems For Fire Management, Dirac L. Twidwell Jr., Craig R. Allen, Carrick Detweiler, James Higgins, Christian Laney, Sebastian Elbaum

Department of Agronomy and Horticulture: Faculty Publications

During the past century, fire management has focused on techniques both to protect human communities from catastrophic wildfire and to maintain fire-dependent ecological systems. However, despite a large and increasing allocation of resources and personnel to achieve these goals, fire management objectives at regional to global scales are not being met. Current fire management techniques are clearly inadequate for the challenges faced by fire managers, and technological innovations are needed. Advances in unmanned aerial systems (UAS) technology provide opportunities for innovation in fire management and science. In many countries, fire management organizations are beginning to explore the potential of UAS …


Soil Water Improvements With The Long-Term Use Of A Winter Rye Covercrop, Andrea D. Basche, Thomas C. Kaspar, Sotirios V. Archontoulis, Dan B. Jaynes, Thomas J. Sauer, Timothy B. Parkin, Fernando E. Miguez Jan 2016

Soil Water Improvements With The Long-Term Use Of A Winter Rye Covercrop, Andrea D. Basche, Thomas C. Kaspar, Sotirios V. Archontoulis, Dan B. Jaynes, Thomas J. Sauer, Timothy B. Parkin, Fernando E. Miguez

Department of Agronomy and Horticulture: Faculty Publications

The Midwestern United States, a region that produces one-third of maize and one-quarter of soybean grain globally, is projected to experience increasing rainfall variability. One approach to mitigate climate impacts is to utilize crop and soil management practices that enhance soil water storage and reduce the risks of flooding as well as drought-induced crop water stress. While some research indicates that a winter cover crop in maize-soybean rotations increases soil water availability, producers continue to be concerned that water use by cover crops will reduce water for a following cash crop. We analyzed continuous in-field soil water measurements from 2008 …


Impact Of Pre-Anthesis Water Deficit On Yield And Yield Components In Barley (Hordeum Vulgare L.) Plants Grown Under Controlled Conditions, Zakaria I. Al-Ajlouni, Ayed Al-Abdallat, Abdul Latief A. Al-Ghzawi, Jamal Y. Ayad, Jamal M. Abu Elenein, Nisreen A. Al-Quraan, P. Stephen Baenziger Jan 2016

Impact Of Pre-Anthesis Water Deficit On Yield And Yield Components In Barley (Hordeum Vulgare L.) Plants Grown Under Controlled Conditions, Zakaria I. Al-Ajlouni, Ayed Al-Abdallat, Abdul Latief A. Al-Ghzawi, Jamal Y. Ayad, Jamal M. Abu Elenein, Nisreen A. Al-Quraan, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Drought at pre-anthesis stages can influence barley growth and results in yield losses. Therefore, it is important to understand how drought at pre-anthesis can affect different traits associated with yield reduction in barley. The objective of this study was to understand the relevance of the genetic background of major flowering time genes in barley plants subjected to pre-anthesis drought and its impact on yield and yield components. A glasshouse experiment using a Randomized Complete Block Design was conducted to investigate the effect of drought and its timing on yield and yield components on eleven barley genotypes, which were selected to …


Shrubland Resilience Varies Across Soil Types: Implications For Operationalizing Resilience In Ecological Restoration, Carissa L. Wonkka, Dirac L. Twidwell, Jacob B. West, William E. Rogers Jan 2016

Shrubland Resilience Varies Across Soil Types: Implications For Operationalizing Resilience In Ecological Restoration, Carissa L. Wonkka, Dirac L. Twidwell, Jacob B. West, William E. Rogers

Department of Agronomy and Horticulture: Faculty Publications

In ecosystems with alternative stable states, restoration success can be thought of as overcoming the resilience of an undesirable state to promote an alternative state that yields greater ecosystem services. Since greater resilience of undesirable states translates into reduced restoration potential, quantifying differences in resilience can enhance restoration planning. In the context of shrub- encroached rangeland restoration, shrubland resilience is the capacity of a woody vegetated state to absorb management interventions designed to produce a more desirable grass- dominated state, and remain within its current regime. Therefore, differences in the resilience of a state can be quantifi ed in a …


Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding Jan 2016

Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such …


Letter To The Editor Ws-2 Introgression In A Proportion Of Arabidopsis Thaliana Col-0 Stock Seed Produces Specific Phenotypes And Highlights The Importance Of Routine Genetic Verification, Mon-Ray Shao, Vikas Shedge, Hardik Kundariya, Fredric R. Lehle, Sally Ann Mackenzie Jan 2016

Letter To The Editor Ws-2 Introgression In A Proportion Of Arabidopsis Thaliana Col-0 Stock Seed Produces Specific Phenotypes And Highlights The Importance Of Routine Genetic Verification, Mon-Ray Shao, Vikas Shedge, Hardik Kundariya, Fredric R. Lehle, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Arabidopsis thaliana is an important model organism with a robust network of resources that has been of enormous value to the plant science research community. The use of isogenic material as a reference point or control is critical for many types of experiments in plant molecular biology and genetics. Recently, we noticed that some seed from a common source of the widely-used Columbia-0 (Col-0) strain gave rise to plants showing features atypical for this strain. Whole genome DNA-sequencing and allele-specific PCR assays confirmed that the abnormal individuals contain multiple introgressions from the ecotype Wassilewskija-2 (Ws-2), as described below. This emphasizes …


Construction Of High Resolution Genetic Linkage Maps To Improve The Soybean Genome Sequence Assembly Glyma1.01, Qijian Song, Jerry Jenkins, Gaofeng Jia, David L. Hyten, Vince Pantalone, Scott A. Jackson, Jeremy Schmutz, Perry B. Cregan Jan 2016

Construction Of High Resolution Genetic Linkage Maps To Improve The Soybean Genome Sequence Assembly Glyma1.01, Qijian Song, Jerry Jenkins, Gaofeng Jia, David L. Hyten, Vince Pantalone, Scott A. Jackson, Jeremy Schmutz, Perry B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Background: A landmark in soybean research, Glyma1.01, the first whole genome sequence of variety Williams 82 (Glycine max L. Merr.) was completed in 2010 and is widely used. However, because the assembly was primarily built based on the linkage maps constructed with a limited number of markers and recombinant inbred lines (RILs), the assembled sequence, especially in some genomic regions with sparse numbers of anchoring markers, needs to be improved. Molecular markers are being used by researchers in the soybean community, however, with the updating of the Glyma1.01 build based on the high-resolution linkage maps resulting from this research, …


Muts Homolog1 Silencing Mediates Orf220 Substoichiometric Shifting And Causes Male Sterility In Brassica Juncea, Na Zhao, Xinyue Xu, Yashitola Wamboldt, Sally Ann Mackenzie, Xiaodong Yang, Zhongyuan Hu, Jinghua Yang, Mingfang Zhang Jan 2016

Muts Homolog1 Silencing Mediates Orf220 Substoichiometric Shifting And Causes Male Sterility In Brassica Juncea, Na Zhao, Xinyue Xu, Yashitola Wamboldt, Sally Ann Mackenzie, Xiaodong Yang, Zhongyuan Hu, Jinghua Yang, Mingfang Zhang

Department of Agronomy and Horticulture: Faculty Publications

Cytoplasmic male sterility (CMS) has consistently been associated with the expression of mitochondrial open reading frames (ORFs) that arise from genomic rearrangements. Spontaneous fertility reversion in CMS has been observed in several cases, but a clear understanding of fertility reversion controlled by nuclear genetic influences has been lacking. Here, we identified spontaneous fertile revertant lines for Brassica juncea CMS cytoplasm in which the mitochondrial genome has undergone substoichiometric shifting (SSS) to suppress ORF220 copy number. We placed ORF220, with or without a mitochondrial targeting presequence, under the control of the CaMV35S and AP3 promoters in Arabidopsis to confirm that ORF220 …


Growing Sensitivity Of Maize To Water Scarcity Under Climate Change, Qingfeng Meng, Xinping Chen, David B. Lobell, Zhenling Cui, Yi Zhang, Haishun Yang, Fusuo Zhang Jan 2016

Growing Sensitivity Of Maize To Water Scarcity Under Climate Change, Qingfeng Meng, Xinping Chen, David B. Lobell, Zhenling Cui, Yi Zhang, Haishun Yang, Fusuo Zhang

Department of Agronomy and Horticulture: Faculty Publications

Climate change can reduce crop yields and thereby threaten food security. The current measures used to adapt to climate change involve avoiding crops yield decrease, however, the limitations of such measures due to water and other resources scarcity have not been well understood. Here, we quantify how the sensitivity of maize to water availability has increased because of the shift toward longer-maturing varieties during last three decades in the Chinese Maize Belt (CMB). We report that modern, longer-maturing varieties have extended the growing period by an average of 8 days and have significantly offset the negative impacts of climate change …


Information Thermodynamics Of Cytosine Dna Methylation, Robersy Sanchez Rodriguez, Sally Ann Mackenzie Jan 2016

Information Thermodynamics Of Cytosine Dna Methylation, Robersy Sanchez Rodriguez, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Cytosine DNA methylation (CDM) is a stable epigenetic modification to the genome and a widespread regulatory process in living organisms that involves multicomponent molecular machines. Genome-wide cytosine methylation patterning participates in the epigenetic reprogramming of a cell, suggesting that the biological information contained within methylation positions may be amenable to decoding. Adaptation to a new cellular or organismal environment also implies the potential for genome-wide redistribution of CDM changes that will ensure the stability of DNA molecules. This raises the question of whether or not we would be able to sort out the regulatory methylation signals from the CDM background …


Horizontal Gene Acquisitions, Mobile Element Proliferation, And Genome Decay In The Host-Restricted Plant Pathogen Erwinia Tracheiphila, Lori R. Shapiro, Erin D. Scully, Timonthy J. Straub, Jihye Park, Andrew G. Stephenson, Gwyn A. Beattie, Mark L. Gleason, Roberto Kolter, Miguel C. Coelho, Consuelo M. De Moraes, Mark C. Mescher, Olga Zhaxybayeva Jan 2016

Horizontal Gene Acquisitions, Mobile Element Proliferation, And Genome Decay In The Host-Restricted Plant Pathogen Erwinia Tracheiphila, Lori R. Shapiro, Erin D. Scully, Timonthy J. Straub, Jihye Park, Andrew G. Stephenson, Gwyn A. Beattie, Mark L. Gleason, Roberto Kolter, Miguel C. Coelho, Consuelo M. De Moraes, Mark C. Mescher, Olga Zhaxybayeva

Department of Agronomy and Horticulture: Faculty Publications

Modern industrial agriculture depends on high-density cultivation of genetically similar crop plants, creating favorable conditions for the emergence of novel pathogens with increased fitness in managed compared with ecologically intact settings. Here, we present the genome sequence of six strains of the cucurbit bacterial wilt pathogen Erwinia tracheiphila (Enterobacteriaceae) isolated from infected squash plants in New York, Pennsylvania, Kentucky, and Michigan. These genomes exhibit a high proportion of recent horizontal gene acquisitions, invasion and remarkable amplification of mobile genetic elements, and pseudogenization of approximately 20% of the coding sequences. These genome attributes indicate that E. tracheiphila recently emerged as a …


Herbicide Options For Effective Weed Management In Dry Directseeded Rice Under Scented Rice-Wheat Rotation Of Western Indo- Gangetic Plains, Vijay Singh, Mangi L. Jat, Zahoor A. Ganie, Bhagirath S. Chauhan, Raj K. Gupta Jan 2016

Herbicide Options For Effective Weed Management In Dry Directseeded Rice Under Scented Rice-Wheat Rotation Of Western Indo- Gangetic Plains, Vijay Singh, Mangi L. Jat, Zahoor A. Ganie, Bhagirath S. Chauhan, Raj K. Gupta

Department of Agronomy and Horticulture: Faculty Publications

Farmers' participatory field trials were conducted at Madhuban, and Taraori, the two participatory experimental sites/locations of the Cereal Systems Initiative for South Asia (CSISA), a collaborative project of IRRI and CIMMYT in Karnal district of Haryana, India, during Kharif (wet season) 2010 and 2011. This research aimed to evaluate preemergence (PRE) and postemergence (POST) herbicides for providing feasible and economically viable weed management options to farmers for predominant scented rice varieties. Treatments with pendimethalin PRE fb bispyribac-sodium þ azimsulfuron POST had lower weed biomass at 45 days after sowing (DAS). At Madhuban, highest grain yield of scented basmati rice (3.43 …


Mapping Qtls And Association Of Differentially Expressed Gene Transcripts For Multiple Agronomic Traits Under Different Nitrogen Levels In Sorghum, Malleswari Gelli, Sharon E. Mitchell, Kan Liu, Thomas E. Clemente, Donald P. Weeks, Chi Zhang, David R. Holding, Ismail M. Dweikat Jan 2016

Mapping Qtls And Association Of Differentially Expressed Gene Transcripts For Multiple Agronomic Traits Under Different Nitrogen Levels In Sorghum, Malleswari Gelli, Sharon E. Mitchell, Kan Liu, Thomas E. Clemente, Donald P. Weeks, Chi Zhang, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Sorghum is an important C4 crop which relies on applied Nitrogen fertilizers (N) for optimal yields, of which substantial amounts are lost into the atmosphere. Understanding the genetic variation of sorghum in response to limited nitrogen supply is important for elucidating the underlying genetic mechanisms of nitrogen utilization.

Results: A bi-parental mapping population consisting of 131 recombinant inbred lines (RILs) was used to map quantitative trait loci (QTLs) influencing different agronomic traits evaluated under normal N (100 kg.ha−1 fertilizer) and low N (0 kg.ha−1 fertilizer) conditions. A linkage map spanning 1614 cM was developed …