Other Plant Sciences Commons^™

Extension Agriculture And Natural Resources In The U.S. Midwest: A Review And Analysis Of Challenges And Future Opportunities, Mahdi M. Al-Kaisi, Roger Wesley Elmore, Gerald A. Miller, David Kwaw-Mensah

Department of Agronomy and Horticulture: Faculty Publications

This review addresses key changes in U.S. agricultural extension and future opportunities. Agricultural extension has been a part of the land-grant university (LGU) system for more than 100 years. The Morrill Act of 1862 established the LGU system by authorizing states access to federally controlled land and funding for public institutions offering educational opportunities focusing on agriculture and mechanical arts for farmers and the working class. Current surveys in Iowa reveal changing trends in extension: more than 90% of farmers identified private-sector crop advisers as their primary source for recommendations, whereas more than 80% of those advisors identified Iowa State …

Soybean Yield And Nodulation Response To Crop History And Inoculation, Stephen Mason, Tomie Galusha, Zaher Kmail

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] inoculation was imposed on a long-term continuous grain sorghum [Sorghum bicolor (L.) Moench] and soybean cropping systems study with and without manure application at Mead, NE. The objective was to determine the influence of 28-yr history of continuous grain sorghum and soybean production, inoculation, and manure on soybean yield and nodulation. Average 2-yr soybean grain yield was 0.5 Mg ha^–1 greater on plots with continuous grain sorghum crop history rather than soybean history, even after this history was broken by 2-yr crop rotation with grain sorghum in the 2 yr previous to …

Soil Moisture Affects Growing-Season Wildfire Size In The Southern Great Plains, Erik S. Krueger, Tyson E. Ochsner, David M. Engle, J. D. Carlson, Dirac L. Twidwell, Samuel D. Fuhlendorf

Department of Agronomy and Horticulture: Faculty Publications

The increasing availability of soil moisture data presents an opportunity for its use in wildfire danger assessments, but research regarding the influence of soil moisture on wildfires is scarce. Our objective was to identify relationships between soil moisture and wildfire size for Oklahoma wildfires during the growing (May-October) and dormant seasons (November-April). We hypothesized that soil moisture influences wildfire size when vegetation is growing but is less important when most vegetation is dead or dormant. Soil moisture, as fraction of available water capacity (FAW), and commonly measured weather variables were determined for 38,419 wildfires from 2000–2012. Wildfires were grouped by …

Relative Salt Tolerance Of Seven Strawberry Cultivars, Youping Sun, Genhua Niu, Russ Wallace, Joseph Masabni, Mengmeng Gu

Plants, Soils, and Climate Faculty Publications

Strawberry (Fragaria × ananassa) cultivars (“Albion”, “Benicia”, “Camarosa”, “Camino Real”, “Chandler”, “Radiance”, and “San Andreas”) were evaluated for salt tolerance in a greenhouse environment. Plants were irrigated with a nutrient solution with an electrical conductivity (EC) of 1.1 dS·m−1 (control) or a nutrient solution with the addition of salts (salt solution) with ECs of 2.2, 3.3, or 4.4 dS·m−1 for four months. Salinity reduced plant growth and fruit yield of strawberry; however, the magnitude of reduction varied with cultivar. For example, at an EC of 4.4 dS·m−1 , “Benicia” and “Chandler” had 39% and 44% less shoot dry weight (DW) …

Demography And Disease Of The Rare Shrub Buckleya Distichophylla (Santalaceae) In Northeastern Tennessee, William Seth Ratliff

Undergraduate Honors Theses

Piratebush (Buckleya distichophylla (Nutt.) Torr.) is a rare, hemiparasitic shrub with the only extant populations in western North Carolina, northeastern Tennessee, and southwestern Virginia. The preferred natural hosts of piratebush, Carolina and eastern hemlocks, have seen sharp declines over the last decade due to the invasive hemlock woolly adelgid. Virginia pine, another important host of piratebush, is also susceptible to disease, specifically Cronartium appalachianum, a rust fungus for which piratebush is the secondary host. This study described and analyzed current demographic parameters of three Tennessee piratebush populations. Additionally, spatial patterns of disease and demographic characters were analyzed. These …

Plant Community Responses To Invasive Shrub And Vine Removal In An Urban Park Woodland., Eric Richard Moore

Electronic Theses and Dissertations

Counter to what some people think, urban areas can be biodiversity hotspots. Maintaining this biodiversity can be challenging, since exotic shrubs and vines block sunlight and threaten native plant regeneration. Since 2007, the Louisville Olmsted Parks Conservancy (LOPC) has spent $2 million on invasive plant management in Cherokee Park. Before the project began, long-term transects were established by the LOPC to collect baseline presence/absence data on 11 invasive plant species. In 2014, I revisited these transects and documented presence/absence data on the entire plant community. I found that four species (garlic mustard, winter creeper, Japanese honeysuckle, and English ivy) have …

Distribution Of Herbicide-Resistant Shattercane And Johnsongrass Populations In Sorghum Production Areas Of Nebraska And Northern Kansas, Rodrigo Werle, Amit J. Jhala, Melinda K. Yerka, J. Anita Dille, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Overreliance on acetolactate synthase (ALS)-inhibiting herbicides for weed control during the 1990s resulted in selection of ALS-resistant shattercane [Sorghum bicolor (L.) Moench ssp. drummondii (Nees ex Steud.) de Wet ex Davidse] biotypes in Nebraska. The objective of this study was to assess the baseline presence of ALS-resistance in 190 shattercane and 59 johnsongrass [Sorghum halepense (L.) Pers.] populations collected across northern Kansas, northwestern Missouri, and southern Nebraska in 2013. In 2014, a preliminary field experiment was conducted to evaluate the presence of herbicide resistance in the aforementioned populations. Treatments consisted of four herbicides (clethodim {2-[1-[[(E)-3-chloroprop-2-enoxy]amino] propylidene]-5-(2-ethylsulfanylpropyl)cyclohexane-1,3-dione}, glyphosate [N-(phosphonomethyl) …

Genetic Control Of Morphometric Diversity In The Maize Shoot Apical Meristem, Samuel Leiboff, Xianran Li, Heng-Cheng Hu, Natalie Todt, Jinliang Yang, Xiao Li, Xiaoqing Yu, Gary J. Muehlbauer, Marja C.P. Timmermans, Jianming Yu, Patrick S. Schnable, Michael J. Scanlon

Department of Agronomy and Horticulture: Faculty Publications

The maize shoot apical meristem (SAM) comprises a small pool of stem cells that generate all above-ground organs. Although mutational studies have identified genetic networks regulating SAM function, little is known about SAM morphological variation in natural populations. Here we report the use of high-throughput image processing to capture rich SAM size variation within a diverse maize inbred panel. We demonstrate correlations between seedling SAM size and agronomically important adult traits such as flowering time, stem size and leaf node number. Combining SAM phenotypes with 1.2 million single nucleotide polymorphisms (SNPs) via genome-wide association study reveals unexpected SAM morphology candidate …

Residue Harvest Effects On Irrigated, No-Till Corn Yield And Nitrogen Response, Charles S. Wortmann, Charles A. Shapiro, Marty R. Schmer

Department of Agronomy and Horticulture: Faculty Publications

Crop residue harvest occurs on about 40% of Nebraska’s 3,700,000 ha of corn (Zea mays L.) land, primarily for feeding of beef cattle. Immobilization of applied N is expected to be less with residue harvest due to reduced microbial activity for digestion of high C/N ratio organic material. Residue reduction may affect subsequent crop yield and response to applied N. Field research was conducted at three locations over 2 yr in eastern Nebraska for irrigated, no-till corn following corn to determine residue harvest effects on yield and the economically optimal nitrogen rate (EONR). Study sites had deep silt loam …

Herbicides Applied At Or Shortly After Seeding Are Effective For Weed Control In Seedling Buffalograss, Luqi Li, Matthew D. Sousek, Zachary Reicher

Department of Agronomy and Horticulture: Faculty Publications

Herbicides applied shortly after seeding of buffalograss [Buchloe dactyloides (Nutt.) Engelm.] can help reduce weed pressure and maximize establishment of buffalograss. This study evaluated 12 relatively recently developed herbicides for turf safety and weed control when applied at seeding or 0 or 2 weeks after emergence (WAE) of ‘Bowie’ or ‘Sundancer’ buffalograss. Primary weed species on the site were common purslane (Portulaca oleracea L.), redroot pigweed (Amaranthus retroflexus L.), and/or yellow foxtail [Setaria lutescens (Weigel ex Stuntz) F.T. Hubb.]. Regardless of cultivar, untreated checks had53% weed cover by 6 WAE, whereas most of the herbicide treatments resulted …

Synfind: Compiling Syntenic Regions Across Any Set Of Genomes On Demand, Haibao Tang, Matthew D. Bomhoff, Evan Briones, Liangsheng Zhang, James C. Schnable, Eric Lyons

Department of Agronomy and Horticulture: Faculty Publications

The identification of conserved syntenic regions enables discovery of predicted locations for orthologous and homeologous genes, evenwhennosuchgeneispresent.Thiscapabilitymeansthatsynteny-basedmethodsarefarmoreeffectivethansequencesimilaritybased methods in identifying true-negatives, a necessity forstudying gene loss and gene transposition. However, the identification of syntenicregionsrequirescomplexanalyseswhichmustberepeatedforpairwisecomparisonsbetweenanytwospecies.Therefore,as the number of published genomes increases, there is a growing demand for scalable, simple-to-use applications to perform comparative genomic analyses that cater to both gene family studies and genome-scale studies. We implemented SynFind, a web-based tool that addresses this need. Given one query genome, SynFind is capable of identifying conserved syntenic regions in any set of targetgenomes.SynFindiscapableofreportingper-geneinformation,usefulforresearchersstudyingspecificgenefamilies,aswellas genome-wide data sets of syntenic gene and predicted gene …

A Population Structure And Genome-Wide Association Analysis On The Usda Soybean Germplasm Collection, Nonoy Bandillo, Diego Jarquin, Qijian Song, Randall L. Nelson, Perry Cregan, James Specht, Aaron Lorenz

Department of Agronomy and Horticulture: Faculty Publications

Population structure analyses and genome-wide association studies (GWAS) conducted on crop germplasm collections provide valuable information on the frequency and distribution of alleles governing economically important traits. The value of these analyses is substantially enhanced when the accession numbers can be increased from ~1,000 to ~10,000 or more. In this research, we conducted the first comprehensive analysis of population structure on the collection of 14,000 soybean accessions [Glycine max (L.) Merr. and G. soja Siebold & Zucc.] using a 50KSNP chip. Accessions originating from Japan were relatively homogenous and distinct from the Korean accessions. As a whole, both Japanese and …

Chromatin Immunoprecipitation (Chip) Assay For Detecting Direct And Indirect Protein -Dna Interactions In Magnaporthe Oryzae, Gang Li, Margarita Marroquin-Guzman, Richard A. Wilson

Department of Plant Pathology: Faculty Publications

Chromatin immunoprecipitation (ChIP) is a powerful technology for analyzing protein-DNA interactions in cells. Robust ChIP procedures have been established for investigating direct interactions between protein and DNA. However, detecting indirect protein-DNA interactions in vivo is challenging. Recently, we used ChIP to analyze an indirect protein-DNA interaction between a putative histone demethylase, MoJmjC, and the promoter of the superoxide dismutase 1-encoding gene MoSOD1 in the rice blast fungus Magnaporthe oryzae (M. oryzae) (Fernandez et al., 2014). We tagged MoJmjC with the 3x FLAG epitope (Fernandez et al., 2014), instead of the larger and more commonly used GFP epitope, to mitigate …

Transgenic Soybean Plants Expressing Asoybean Homolog Of Glycine-Rich Protein 7 (Grp7) And Exhibiting Improved Innate Immunity, James R. Alfano, Anna Joe, Thomas E. Clemente, Zhengqing Fu, Ming Guo, Byeong-Ryool Jeong, Thomas Elthon

Department of Plant Pathology: Faculty Publications

This disclosure provides for transgenic Soybean plants expressing a soybean homolog of glycine-rich protein 7 (GRP7) and exhibiting improved innate immunity and meth ods of making Such plants.

Adding Genetically Distant Individuals To Training Populations Reduces Genomic Prediction Accuracy In Barley, Aaron Lorenz, Kevin P. Smith

Department of Agronomy and Horticulture: Faculty Publications

One of the most important factors affecting genomic prediction accuracy appears to be training population (TP) composition. The objective of this study was to evaluate the effect of genomic relationship on genomic prediction accuracy and determine if adding increasingly unrelated individuals to a TP can reduce prediction accuracy. To accomplish this, a population of barley (Hordeum vulgare L.) lines from the University of Minnesota (lines denoted as MN) and North Dakota State University (lines denoted as ND) breeding programs were used for model training. Predictions were validated using two independent sets of progenies derived from MN  MN crosses …

Community Seed Banks: Origins, Evolution, And Prospects, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

As a survey of contemporary community-level seed banks, this is an excellent compilation and instructive guide. The first section provides an overview of how seed banks originated, their varied goals and activities, several contrasting forms of management, and how they organize and perform to meet these goals. An intriguing claim in the book is that the concept of “seed banks is only some three decades old,” while in fact the practice of saving, preserving, and exchanging seed within a community is probably as old as human communities themselves.

Organized seed banks often serve specific functions: preserving seeds, providing seed access …

Switchgrass Germplasm Resources, Michael D. Casler, Kenneth P. Vogel, Melanie Harrison

Department of Agronomy and Horticulture: Faculty Publications

Switchgrass (Panicum virgatum L.) is an important native grass and dominant member of the tallgrass prairie ecosystem. It is used for conservation, restoration, livestock feed production, and bioenergy feedstock production. The purpose of this review is to describe the biological and geographical basis for switchgrass germplasm diversity and to provide a resource for scientists and outreach personnel to find switchgrass germplasm to meet their needs. Upland and lowland ecotypes represent the most important polymorphism in switchgrass, with distinct but overlapping geographic distributions. Variation in ploidy exists within both ecotypes, with 2n = 4x = 36 the dominant ploidy …

The Khmer Software Package: Enabling Efficient Nucleotide Sequence Analysis [Version 1; Referees: 2 Approved, 1 Approved With Reservations], Michael R. Crusoe, Hussien F. Alameldin, Sherine Awad, Elmar Boucher, Adam Caldwell, Reed Cartwright, Amanda Charbonneau, Bede Constantinides, Greg Edvenson, Scott Fay, Jacob Fenton, Thomas Fenzl, Jordan Fish, Leonor Garcia-Gutierrez, Phillip Garland, Jonathan Gluck, Iván González, Sarah Guermond, Jiarong Guo, Aditi Gupta, Joshua R. Herr, Adina Howe, Alex Hyer, Andreas Härpfer, Luiz Irber, Rhys Kidd, David Lin, Justin Lippi, Tamer Mansour, Pamela Mca'nulty, Eric Mcdonald, Jessica Mizzi, Kevin D. Murray, Joshua R. Nahum, Kaben Nanlohy, Alexander Johan Nederbragt, Humberto Ortiz-Zuazaga, Jeramia Ory, Jason Pell, Charles Pepe-Ranney, Zachary N. Russ, Camille Scott, Josiah Seaman, Scott Sievert 38, Jared Simpson, Connor T. Skennerton, James Spencer, Ramakrishnan Srinivasan, Daniel Standage, James A. Stapleton, Susan R. Steinman, Joe Stein, Benjamin Taylor, Will Trimble, Heather L. Wiencko, Michael Wright, Brian Wyss, Qingpeng Zhang, En Zyme, C. Titus Brown, Erich Schwarz

Department of Plant Pathology: Faculty Publications

The khmer package is a freely available software library for working efficiently with fixed length DNA words, or k-mers. khmer provides implementations of a probabilistic k-mer counting data structure, a compressible De Bruijn graph representation, De Bruijn graph partitioning, and digital normalization. khmer is implemented in C++ and Python, and is freely available under the BSD license at https://github.com/dib-lab/khmer/.

Screening Of Olive Cultivars For Tolerance To Fusicladium Oleagineum In South Africa, Zakhele Cyril Msimango

Professional Agricultural Workers Journal

Abstract

This study evaluated commercial olive cultivars for resistance to olive leaf spot (OLS). A growth chamber trial was undertaken with one temperature treatment (16°C) applied during inoculation while a relative humidity (RH) of above 80% was maintained. Plants were kept for 48 hours at 99% RH after inoculation and then moved to shade netted area for disease development at a temperature of 25±5°C. The results were used for categorizing the eight olive cultivars evaluated in this study according to their OLS susceptibility. ‘Frantoio’ was categorized as most tolerant; ‘Nandi’ and ‘Leccino’ were found to be moderately tolerant, and ‘Nocellara …

Prescribed Fire Monitoring Report, Tallgrass Prairie National Preserve 2014 (Iqcs Fire Number 285382, 285383, 266782, 285677), Sherry A. Leis, Sarah E. Hinman

United States National Park Service: Publications

Introduction

In 2014, the preserve’s federal and NGO partners conducted prescribed fires during March, April, and October that encompassed 8129.8 acres of Tallgrass Prairie National Preserve (TAPR). This was a unique burn year in that prescribed burns occurred in the spring, the traditional burn season, and the fall. Fall burns were conducted to support needed archaeological surveys as part of the environmental compliance for a symphony event scheduled for June 2015 at the preserve. Burns at TAPR were coordinated with local US Fish and Wildlife Service (USFWS), The Nature Conservancy (TNC), and various units of the National Park Service.

Burns …

A Unique 5' Translation Element Discovered In Triticum Mosaic Virus, Robyn Roberts, Jincan Zhang, Laura K. Mayberry, Satyanarayana Tatineni, Karen S. Browning, Aurelie M. Rakotondrafara

Department of Plant Pathology: Faculty Publications

Several plant viruses encode elements at the 5' end of their RNAs, which, unlike most cellular mRNAs, can initiate translation in the absence of a 5' m7GpppG cap. Here, we describe an exceptionally long (739-nucleotide [nt]) leader sequence in triticum mosaic virus (TriMV), a recently emerged wheat pathogen that belongs to the Potyviridae family of positive-strand RNA viruses. We demonstrate that the TriMV 5' leader drives strong cap-independent translation in both wheat germ extract and oat protoplasts through a novel, noncanonical translation mechanism. Translation preferentially initiates at the 13th start codon within the leader sequence independently of eIF4E but involves …

Community Involvement To Address A Long-Standing Invasive Species Problem: Aspects Of Civic Ecology In Practice, Rebecca W. Dolan, Kelly Harris, Mark Adler

Scholarship and Professional Work - LAS

Invasive non-native species (INS) are found in every city around the globe, but their impacts in urban settings as biological agents of visual pollution that block views of natural landscapes and disconnect citizens from nature are not as often addressed as comprehensively as their impacts in natural areas or agricultural settings. The multiple impacts of INS in cities make them ideal candidates for aspects of Civic Ecology Practice, where local environmental stewardship action is taken to enhance green infrastructure and community well-being in urban and other human-dominated systems. We present details of a community driven program focused on removal of …

Community Involvement To Address A Long-Standing Invasive Species Problem: Aspects Of Civic Ecology In Practice, Rebecca W. Dolan, Kelly Harris, Mark Adler

Rebecca W. Dolan

Invasive non-native species (INS) are found in every city around the globe, but their impacts in urban settings as biological agents of visual pollution that block views of natural landscapes and disconnect citizens from nature are not as often addressed as comprehensively as their impacts in natural areas or agricultural settings. The multiple impacts of INS in cities make them ideal candidates for aspects of Civic Ecology Practice, where local environmental stewardship action is taken to enhance green infrastructure and community well-being in urban and other human-dominated systems. We present details of a community driven program focused on removal of …

Sources Of Heat In Synthetic Turf Systems, Adam William Thoms

Doctoral Dissertations

With options for cooling synthetic turf limited, an outdoor experiment was conducted at the University of Tennessee Center for Athletic Field Safety (Knoxville, TN) to develop a model to predict maximum, minimum, and mean synthetic turf surface temperature using forecasted atmospheric conditions. Synthetic turf surface temperature varied due to both air temperature and solar radiation. Predictive models using these data accounted for 86, 95, and 94% of the variation in daily maximum, minimum, and mean synthetic turf surface temperature. Accuracy of these models for predicting daily mean and minimum synthetic turf surface temperature using 48 and 72-hour forecasted air temperature …

Occurrence Of An Herbicide-Resistant Plant Trait In Agricultural Field Margins, Karla L. Gag, David J. Gibson, Bryan G. Young, Julie M. Young, Joseph L. Matthews, Stephen C. Weller, Robert G. Wilson

Department of Agronomy and Horticulture: Faculty Publications

Agricultural environments allow study of evolutionary change in plants. An example of evolution within agroecological systems is the selection for resistance to the herbicide glyphosate within the weed, Conyza canadensis. Changes in survivorship and reproduction associated with the development of glyphosate resistance (GR) may impact fitness and influence the frequency of occurrence of the GR trait. We hypothesized that site characteristics and history would affect the occurrence of GR C. canadensis in field margins. We surveyed GR occurrence in field margins and asked whether there were correlations between GR occurrence and location, crop rotation, GR crop trait rotation, crop type, …

Map Supplements For The Metallic Woodboring Beetles (Coleoptera: Buprestidae) Of Tennessee, William E. Klingeman Iii, Jason A. Hansen, Joshua P. Basham, Jason B. Oliver, Nadeer N. Youssef, Whitney Swink, Christine A. Nalepa, Donna C. Fare, J. Kevin Moulton

Plant Sciences Publications and Other Works

Following compilation of distribution records and seasonal flight activity information for 106 metallic wood-boring beetle (Coleoptera: Buprestidae) species for Tennessee, maps were updated to provide county-level collection notations for 10,543 published records and specimen collections made from 1934 to 2013. County collection tallies across the ecoregions in Tennessee are also presented. Maps for individual species highlight gaps in key geographic areas where specimens have not been collected and can be valuable for future biosurveillance, monitoring and management efforts for these economically and ecologically important insects.

Comparing Yield Monitors With Weigh Wagons For On-Farm Corn Hybrid Evaluation, Bjorn P. Nelson, Roger Wesley Elmore, Andrew W. Lenssen

Department of Agronomy and Horticulture: Faculty Publications

For many years, on-farm yield evaluations of corn (Zea mays L.) hybrids were done with weigh wagons, handheld moisture testers, and measuring wheels. Today, most combines have continuous flow yield and moisture sensors. Published research results comparing the accuracy of combine-mounted sensor systems with that of weigh wagons are limited for on-farm corn hybrid evaluation. This study examined the accuracy of combine-mounted yield sensors with traditional weigh wagon methodology in on-farm corn hybrid strip trials. Data from combine-mounted sensors for plot weight, moisture percentage, and yield were compared with weigh wagon weight, handheld moisture testers, and calculated yield …

Genetic Engineering Online Lessons Improve Teaching And Increase Knowledge And Accepting Attitudes Among Students, Grace Troupe

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Genetic engineering has been used in the production of food in the U.S. for nearly three decades, however, science literacy in genetic engineering among consumers is still low. To address this problem, an online resource called The Journey of a Gene (passel.unl.edu/ge) was created to help incorporate genetic engineering education in high school and college curriculums. Here we report two studies conducted to evaluate the effectiveness of The Journey of a Gene in 1) improving student knowledge and attitudes about genetic engineering and 2) helping teachers increase their knowledge as well as quantity and quality of genetic engineering instruction. In …

Genetic Diversity Of Miscanthus In The Us Naturalized Populations Based On Molecular Marker, Suma Basak

College of Agriculture, Environment and Nutrition Sciences Graduate Theses and Dissertations

ABSTRACT

Miscanthus is a fast growing bioenergy grass, tolerant of adverse conditions and has high biomass production, and is increasingly attractive as a potential source of cellulosic ethanol. An understanding of genetic variation in naturalized populations of Miscanthus in the US may help in selecting appropriate parents for breeding superior cultivars that are adapted in the wide-ranging and changing environments. The molecular marker analysis of Miscanthus is an important tool towards the genetic improvement program of this bioenergy grass. As sugarcane is closely related to Miscanthus, we employed its DNA markers as probes to explore genetic diversity within and among …

Forest Sustainability In An Altered Landscape Requires Human Intervention, Adam C. Labatore

Biology Theses

Recruitment success determines the abundance and composition of plant communities. Successful recruitment can be summarized as a simple dichotomy of establishment versus seed limitations. Establishment limitation occurs when plant populations are inhibited by habitat availability and quality, whereas seed limitation occurs when seeds fail to arrive in necessary densities. Recruitment failure undermines overstory sustainability if the existing trees cannot replace themselves. Preliminary analysis indicated tree recruitment failure at an urban nature preserve with too few tree seedlings to replace mature canopy trees. These data suggested that the urban forest was not sustainable. The overarching goal of the study was to …