Other Plant Sciences Commons^™

Accumulation Of A 5′ Proximal Subgenomic Rna Of Citrus Tristeza Virus Is Correlated With Encapsidation By The Minor Coat Protein, Siddarame Gowda, Satyanarayana Tatineni, Svetlana Y. Folimonova, Mark E. Hilf, William O. Dawson

Department of Plant Pathology: Faculty Publications

During replication, Citrus tristeza virus (CTV) produces large amounts of two unusual subgenomic (sg) RNAs that are positive-stranded and 5′ coterminal. Although these RNAs are produced in similar amounts and are similar in size, with LMT1 (~750 nt) only slightly larger than LMT2 (~650), we found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the LMT2 RNA occurred late, coinciding with virion accumulation. The long …

Choline-Utilizing Microblal Strains For Biologically Controlling Fusarium Head Blight, David A. Schisler, Naseem I. Khan, Michael J. Boehm, Patricia J. Slininger

Department of Plant Pathology: Faculty Publications

Three choline utilizing strains of microorganisms isolated from the anthers of wheat, Aureobasidium pullulans strainAS 55.2, Arthrobacter species strain OH 221.3, and Pseudomonas species strain AS 64.4, are superior antagonists of F. graminearum. These microorganisms are effective for suppression and control of FHB in cereals, particularly in wheat and barley.

Dollar Spot Fungus Sclerotinla Homoeocarpa Produces Oxalic Acid, R. C. Venu, Robert A. Beaulieu, Terrance L. Graham, Ainhoa Martinez Medina, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Dollar spot, caused by Sclerotinia homoeocarpa,. is one of the most devastating diseases of turfgrass worldwide. Many fungi belonging to the genus Sclerotinia produce oxalic acid along with pectolytic cell wall-degrading enzymes. A series of in vitro experiments showed the relationships among temperature, pH, mycelial growth and acid production. Mycelial growth and acid production were most abundant when S. homoeocarpa was grown between 20 and 30°C. Acid production by S. homoeocarpa appeared to be dependent upon the pH of the environment in which it was grown. High performance liquid chromatography analysis of spent broth revealed the presence of oxalic acid. …

Molecular Characterization Of Citrus Tatter Leaf Virus Historically Associated With Meyer Lemon Trees: Complete Genome Sequence And Development Of Biologically Active In Vitro Transcripts, Satyanarayana Tatineni, Mohammad R. Afunian, Mark E. Hilf, Siddarame Gowda, William O. Dawson, Stephen M. Garnsey

Department of Plant Pathology: Faculty Publications

Citrus tatter leaf virus isolated from Meyer lemon trees (CTLV-ML) from California and Florida induces bud union incompatibility of citrus trees grafted on the widely used trifoliate and trifoliate hybrid rootstocks. The complete genome sequence of CTLV-ML was determined to be 6,495 nucleotides (nts), with two overlapping open reading frames (ORFs) and a poly (A) tail at the 3′ end. The genome organization is similar to other capilloviruses, with ORF1 (nts 37 to 6,354) encoding a putative 242-kDa polyprotein which contains replication-associated domains plus a coat protein (CP), and ORF2 (nts 4,788 to 5,750), which is located within ORF1 in …

Plant-Microbes Interactions In Enhanced Fertilizer-Use Efficiency, Anthony O. Adesemoye, Joseph W. Kloepper

Department of Plant Pathology: Faculty Publications

The continued use of chemical fertilizers and manures for enhanced soil fertility and crop productivity often results in unexpected harmful environmental effects, including leaching of nitrate into groundwater, surface runoff of phosphorus and nitrogen runoff, and eutrophication of aquatic ecosystems. Integrated nutrient management systems are needed to maintain agricultural productivity and protect the environment. Microbial inoculants are promising components of such management systems. This review is a critical summary of the efforts in using microbial inoculants, including plant growth-promoting rhizobacteria and arbuscular mycorrhizal fungi for increasing the use efficiency of fertilizers. Studies with microbial inoculants and nutrients have demonstrated that …

Bulked Segregant Analysis Using The Goldengate Assay To Locate The Rpp3 Locus That Confers Resistance To Soybean Rust In Soybean, D. L. Hyten, James R. Smith, Reid D. Frederick, Mark L. Tucker, Qijian Song, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Few resistance loci to soybean rust (SBR), caused by Phakopsora pachyrhizi Syd., have been genetically mapped and linked to molecular markers that can be used for marker assisted selection. New technologies are available for single nucleotide polymorphism (SNP) genotyping that can be used to rapidly map traits controlled by single loci such as resistance to SBR. Our objective was to demonstrate that the highthroughput SNP genotyping method known as the GoldenGate assay can be used to perform bulked segregant analysis (BSA) to fi nd candidate regions to facilitate effi cient mapping of a dominant resistant locus to SBR designated Rpp3 …

Mapping And Confirmation Of A New Allele At Rpp1 From Soybean Pi 594538a Conferring Rb Lesion–Type Resistance To Soybean Rust, Nanda Chakraborty, Joe Curley, Reid D. Frederick, D. L. Hyten, Randall L. Nelson, Glen L. Hartman, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean rust, caused by Phakopsora pachyrhizi (H. Sydow & Sydow), is a destructive soybean [Glycine max (L.) Merr.] disease and identification of new resistance genes is essential for effective rust management. Our research objectives were to map and confirm the location of resistance gene(s) in PI 594538A using a population of 98 F_3:4 lines from across between PI 594538A (reddish-brown [RB] lesions) and the susceptible cultivar Loda (tan [TAN] lesions). The lines were inoculated with the P. pachyrhizi isolate ZM01-1 from Zimbabwe. The RB resistance in PI 594538A mapped on linkage group G as a single dominant gene. …

Detection And Validation Of Single Feature Polymorphisms Using Rna Expression Data From A Rice Genome Array, Sung-Hyun Kim, Prasanna R. Bhat, Xinping Cui, Harkamal Walia, Jin Xu, Steve Wanamaker, Abdelbagi M. Ismail, Clyde Wilson, Timothy J. Close

Department of Agronomy and Horticulture: Faculty Publications

Background: A large number of genetic variations have been identified in rice. Such variations must in many cases control phenotypic differences in abiotic stress tolerance and other traits. A single feature polymorphism (SFP) is an oligonucleotide array-based polymorphism which can be used for identification of SNPs or insertion/deletions (INDELs) for high throughput genotyping and high density mapping. Here we applied SFP markers to a lingering question about the source of salt tolerance in a particular rice recombinant inbred line (RIL) derived from a salt tolerant and salt sensitive parent. Results: Expression data obtained by hybridizing RNA to an oligonucleotide array …

Antibiotic Use In Animal Production: Environmental Concerns, Teshome H. Regassa, Richard K. Koelsch, Charles S. Wortmann, Richard F. Randle, Akwasi A. Abunyewa

Department of Agronomy and Horticulture: Faculty Publications

Introduction

Antibiotic1 use in animal production has led to improved feed use efficiency and increased growth rates. In turn, these resulted in reduced food production costs and reduced excrement of manure nutrients which may cause pollution problems.

On the other hand, antibiotic use leads to antibiotic resistance and a possible reduction in effectiveness of treatment options for both animals and humans. This publication addresses these issues.

Disease-causing microorganisms2, including bacteria,are the most diverse and numerous organisms on earth. Diverse in their habitat, environmental adaptation, and mechanisms of reproduction, they have short generations with high multiplication rates. Such characteristics help develop …