Other Plant Sciences Commons^™

Center For Grassland Studies Newsletter, Winter 2004, Volume 10, No. 1

Center for Grassland Studies: Newsletters

Matching Beef Cattle Genetics to Feed Resources by Jim Gosey, Department of Animal Science, UNL

Ecological Monitoring to Assist Producers with Grassland Production Forecasts by George Green, School of Natural Resources, UNL

Nebraska Ranch Practicum Enters Sixth Year by Jerry Volesky, Don Adams, Richard Clark, Dillon Feuz, Patrick Reece, Brent Plugge, Bud Stolzenburg and Troy Walz, UNL

Bull Riders for Biodiversity? – And Other Heresies for the Northern Great Plains by Tyler Sutton, for The Conservation Alliance of the Great Plains

2004 Nebraska Grazing Conference to be Aug. 10-11

The Nebraska Turfgrass Conference: 42 Years of Educating Nebraska Turfgrass Managers …

Nebline, November/December 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Local Efforts to Promote Good Health
What Parents Can Do
Tax Planning Update for 2004
Don’t Forget to Winterize Your Underground Sprinkler System
Management Pays Better Than Labor
Management = Planning for the Future
Helping Trees Through Winter
Sources of Man-Made Chemicals in Drinking Water
How to Hire an Arborist
Tips on Storing Firewood
Tips on Turkey Preparation
WINTER FRUIT GLAZE Recipe
How to Cook a Turkey the Day Before Serving It
GREEN ONION AND CORN BREAD STUFFING Recipe
Simple Tip to Avoid Scratches When Storing Nonstick Skillets
Cooking Tools Class Oct. 26
President’s Notes — Janet’s Jargon
Household Hints: …

Center For Grassland Studies Newsletter, Fall 2004, Volume 10, No. 4

Center for Grassland Studies: Newsletters

Nebraska's Natural Legacy Project by Kristal Stoner, Nebraska Game and Parks Commission

Earth, Wind, and Fire in the Flint Hills by David Hartnett, Konza Prairie Biological Station, Kansas State University

Owens Highlights UNL Grazing Activities at Grazing Conference

Professional Golf Management Program Tees Off with 31 Students

CGS Director Massengale Inducted into CSREES Hall of Fame

Nebline, October 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Discover 4-H, Discover You!
Five Ways to Join 4-H!
Nebraska Wild Fruit and Nut Jam Oct. 2
Ten Rules for Planting Trees
Garden Guide: Things to do this month
Direct Seeding Hardwood Tree Species
Storing Sweet Potatoes
Watch for Deer When Driving, Especially at Dawn and Dusk
Head Lice Resources You Can Trust
Nebraska Water Facts
The Hazards of Biting Fleas
Upcoming Household Hazardous Waste Collections
The Hazards of Biting Fleas
Prepare Bins and Equipment Before Harvest
“Grapes” is October Rural Living Clinic
Add Lime This Fall for Next Spring’s Alfalfa
Finding Forgotten Food
National Popcorn Poppin’ Month
Camp Abbott: …

Nebline, September 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

What if You Could Access Extension’s Educational Resources at the Push of a Button?
Sorting Through Internet Clutter
What Users Are Saying
Garden Guide: Things to do this month
Good, Low-Maintenance Trees to Plant
Herbal Festival
Aster Yellows on Purple Coneflower
Ladybugs, Ladybugs, Fly Away Home! Please!
Composting Indoors with Worms
Upcoming Household Hazardous Waste Collections
Packed Lunch Pizzazz!
Sports Mix Recipe
Adding A Rainbow To Your Diet
President’s Notes — Janet’s Jargon
Household Hints: Chill Out with an Efficient Fridge
FCE News & Events
Stress Affects Adolescents Too
Plan Now for Holiday Spending
Values
St. Johnswort is Increasing in …

Nebline, August 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

People’s Choice 4-H Awards
“My Pond Won’t Stay Full!”
Terminating Farm Leases
Put Farm Leases in Writing for Legal Protection
“Ponds” is August Rural Living Clinic
Treating Bee Stings
How Effective are DEET Repellents?
Tree Squirrels - Tricksters and Troublemakers
Found an Underground Yellow Jacket Nest? Proceed with Care!
Summer Fruits and Veggies: Q & A
“Healthy One Dish Dinners for 1, 2, 4 or More”
President’s Notes — Janet’s Jargon
Household Hints: Lower Your Lighting Costs
FCE News & Events
More Tools for Student Success
Helping a Child Ease Back Into School
Getting Ready for the New School Year …

Agribusiness Sheep Updates - 2004 Part 3, Ken Hart, Keith Croker, Rob Davidson, Mario D'Antuono, Evan Burt, Tanya Kilminster, Diana Fedorenko, Darryl Mcclements, Robert Beard, Sandy Turton, Martin Atwell, Robert Beard

Sheep Updates

Posters Can Merino Ewes be Teased to Synchronise Oestrus for a Summer/Autumn Mating? Ken Hart, Keith Croker, Department of Agriculture Western Australia, Rob Davidson, School of Animal Biology, University of Western Australia. Mario D'Antuono, Department of Agriculture Western Australia Damara Sheep: Now Looking Domestic Evan Burt and Tanya Kilminster, Department of Agriculture Western Australia Production and Water Use of Lucerne and French Serradella Under Three Sowing Rates Diana Fedorenko1,4, Darryl McClements1,4 , Robert Beard3,4 1Department of Agriculture, 3 Farmer, Meckering, 4CRC for Plant-based Management of Dryland Salinity. E-SHEEP - Individual Animal Management is here Sandy Turton & Martin Atwell, Department …

Agribusiness Sheep Updates - 2004 Part 2, Anyou Liu, Clinton Revell, Phil Nichols, Brad Nutt, Darryl Clements, Lucy Anderton, Stephen Gherardi, Chris Oldham, Paul Sanford, John Gladman, G. E. Donald, A. Edirisinghe, D. A. Henry, S. P. Gittins, R. C. G. Smith, Roy Butler, Kelly Pearce, David Masters, David Pethick, Andrew Thompson, Ken Hart, Johan Greeff, Beth Paganoni, Rachel Kirby, Matt Ryan, Kira Butler, Roger Heggarty, David Hopkins, Samantha Giles, Tom Plaisted, Mark Ferguson, Darren Gordon, John Young, Sandra Brown, Ian Mcfarland, John Archer, John Milton, Rob Davidson, Graeme Martin, David Lindsay, Johnathan England, Mandy Curnow, Karina P. Wood, Ashley K. White, B. Lloyd Davies, Paul M. Carberry, Mark Hyder, Mike Freer, Andrew Van Burgel, Kazue Tanaka, Andrew Peterson, Roger Wiese, Gonzalo Mata, Evan Burt, Amanda Miller, Anne Bennett, Felicity Flugge, Amir Abadi, Perry Dolling, Dean Thomas, Mike Ewing, David Lindsay, Emma Kopke, E. A. Dowling, E. K. Crossley, Brien (Ben) E. Norton, John Karlsson, Geoff Pollott, Diana Fedorenko, Darryl Clements, Robert Beard, Brown Besier, Una Ryan, Caroline Bath

Sheep Updates

Precision Pastures

Using Species Diversity to Improve Pasture Performance Anyou Liu and Clinton Revell, Department of Agriculture, Western Australia

New Annual Pasture Legumes for Sheep Graziers Phil Nichols, Angelo Loi, Brad Nutt and Darryl McClements Department of Agriculture Western Australia

Pastures from Space – Can Satellite Estimates of Pasture Growth Rate be used to Increase Farm Profit? Lucy Anderton, Stephen Gherardi and Chris Oldham Department of Agriculture Western Australia

Summer-active Perennial Grasses for Profitable Sheep Production Paul Sanford and John Gladman, Department of Agriculture, Western Australia

Pastures From Space – Validation Of Predictions Of Pasture Growth Rates DONALD, G.E^{.A …}

Center For Grassland Studies Newsletter, Summer 2004, Volume 10, No. 3

Center for Grassland Studies: Newsletters

WILD Nebraska Grasslands by Bill Vodehnal, Nebraska Game and Parks Commission

High School Students Improve Their Game While Having Fun at UNL Golf Camp

Multiple Dimensions of a Strong Professional Golf Management Program by Charles Francis, Department of Agronomy and Horticulture, UNL

CGS Advisory Council Tours Northeast Nebraska

Groundwater Nitrates Being Used to Fertilize Intensive Grazed Pasture

Grazing and Grassland Management Is Theme of Wildlife Society Meeting

Regional Researchers Collaborate to Improve Grazing Systems

UNL Researchers Study Seed Production of Eastern Red Cedar

Range Management Focus of Beef Basics VI

Still Time to See Exhibition – Listening to the Prairie …

Nebline, July 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Tips, Tools and Travel-Safe Foods for Summer Food Safety
Frozen Custard Ice Cream Recipe
2004 “Great Plants” Plants of the Year
Black Spot of Rose
Garden Guide
West Nile Virus: Elderly at Risk
Using DEET Repellents Safely
Is your Acreage or Farm a Mosquito-Breeding Site?
Effect of Increasing Energy Prices on Irrigation Costs
Watch for Blister Beetles in Alfalfa
Pesticide Container Recycling
New Rules for CRP—Acres Can Be Used for Hay or Pasture
Pouring Quality Concrete
Drinking Water Bacteria
Control of Muskrat Damage in Ponds
“Private Drinking Water Systems” is July Rural Living Clinic
Cool It! Use a Refrigerator/ Freezer …

Promoters From Chlorella Virus Genes Providing For Expression Of Genes In Prokaryotic And Eukaryotic Hosts, Amitava Mitra, James L. Van Etten

Department of Plant Pathology: Faculty Publications

The invention is directed to novel promoters or mutants thereof from Chlorella virus DNA methyltansferase genes. A Chlorella Virus gene promoter is operably linked to a first and/or Second DNA sequence encoding a gene that is different from the Chlorella virus to form an expression cassette. An expression cassette can be introduced into prokaryotic and/or eukaryotic cells and can provide for a high level of expression of the gene encoded by the first and/or Second DNA sequence. The invention also provides a method for Screening other Chlorella virus genes for promoters that can function to express a heterologous gene in …

Nebline, June 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Better Safe Than Sorry
Safety in the Home
Controlling Bagworms
Propagation of Shrubs from Softwood Cuttings
Garden Guide: Things to do This Month
2004 Perennial Plant of the Year
Brighten Your Outdoor Area With Hanging Baskets
President’s Notes — Janet’s Jargon
Household Hints: Water Saving Measures
More Tools for Student Success
What Has Happened to Family Time?
Respect
Effect of Increasing Energy Prices on Farming Costs
Pesticide Container Recycling
“Small Scale Animal Feeding” is June Rural Living Clinic
The Nebraska LEAD Program
Wheat Plot Tour June 10
Examine Trees For Insect, Disease Problems
Septic Tanks Must Be Pumped By Certified …

Organic Horticulture: Strategic Opportunities In Western Australia, Steven Mccoy

Bulletins 4000 -

This report considers the potential to develop organic horticulture in WA. The study examines key factors limiting industry scale and scope and identifies preferred soil types, regional locations, prospective crops and potential scale where organic production may offer comparative economic advantage.

Center For Grassland Studies Newsletter, Spring 2004, Volume 10, No. 2

Center for Grassland Studies: Newsletters

CGS Coordinates New Professional Golf Management Program

Alternatives to Grazing Drought-Stricken Pastures by Ivan Rush, Panhandle Research and Extension Center, UNL

2004 Nebraska Grazing Conference in Kearney August 10-11

Dave and Loretta Hamilton: Grazing and Ranch Management Mentors by Dana Larsen, Natural Resources Conservation Service, USDA

Loess Hills Conservation Measures Improve Rangeland Conditions and Aid American Burying Beetle in Nebraska by Thomas Walker, Jr., Nebraska Game and Parks Commission Jerry Volesky, Department of Agronomy and Horticulture, UNL Wyatt Hoback, Department of Biology, University of Nebraska at Kearney

XX International Grassland Congress, Ireland, 2005

Allan Savory on Holistic Resource Management at …

Nebline, May 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

UNL Termite Workshops Can Help You Make Better Decisions About Termites
Controlling Yellow Nutsedge
Garden Guide: Things to do this month
Annual Vines in the Landscape
Blue Flowers for the Garden
Garter Snakes are Urban Snakes
Keep Unwanted Bird Flocks from Dropping In
Backyard Wildlife: How to Attract Birds with Nest Material
May is Time to Control Leafy Spurge
Scrap Tire Collection May 15 & 16
Fertilizing Grasses in Pastures
Wheat Plot Tour June 10
Knights of Ak-Sar-Ben Pioneer Farm Family Awards
Pay Attention to Utility Lines When Planting Trees
Slime Flux in Trees
Bittersweet Vines, Colorful But Invasive
Growing …

Nebline, April 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Nose Printing as a Method of Cattle Identification
Eye-Catching Technology
Henbit or Ground Ivy- How to Tell the Difference
Night-Blooming Flowers
Garden Guide: Things To Do This Month
Growing Parsley
Competing with Ants for Kitchen Counter Space? You May Have Odorous House Ants
Everything Homeowners Need to Know About Termite Control
Spring Turkey Hunting Workshop
2004 Household Hazardous Waste Collections
Look For Vole Damage
Fertilizing Grass Pastures and Hay Lands
Liming Acid Soils – pH and Buffer pH
Scrap Tire Collection May 15 & 16
Give Trees a Chance with Proper Weed Control
Utility Tractor Recall
Daily Tractor Maintenance
Use …

Nebline, March 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

All-American Selections: 72 Years of Award-Winning Plants
Nature, the Ultimate Recycler
Scrap Tire Collection Announced
Guidelines for Farm Debt
Roosting Starlings are a Nasty Problem
UNL Beekeeping Programs for 2004
Cats in the Neighborhood Can be a Nuisance, Harmful to Local Wildlife
Ants on the Web
Eating GREEN on St. Patrick’s Day
Easy PB & Banana Waffles Recipe
Oh Those Darn Portion Sizes
Tea Time! - Brewing a Tasty (& Healthy!) Cup of Tea
Eating Dairy Products May Help Children Control Their Weight
President’s Notes – Janet’s Jargon
Household Hints
FCE News & Events
National Poison Prevention Week is March …

Nebline, February 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Lancaster County Benefits from a Year-Round Farmer’s Market
Computerized Financial Record Keeping Workshops
Check Condition of Stored Grain
The Dream of Country Living
Attracting Birds with Proper Habitat
Urban Agriculture is Branch of Modern Sustainable Agriculture
Power Tool Safety
See “Red” on Valentine’s Day
Strawberry Sauce Recipe
Golden Apple Oatmeal Recipe
A Healthy Plate - What Does It Look Like?
No-Salt Sloppy Joe Seasoning Mix
President’s Notes – Janet’s Jargon
Household Hints: Keep Heating & Cooling Costs Under Control
FCE News & Events
Sharing Meals Important to Building Family Unity
Ease Parenting During Teen Years With Communication, Involvement
A Caring …

Closterovirus Bipolar Virion: Evidence For Initiation Of Assembly By Minor Coat Protein And Its Restriction To The Genomic Rna 5' Region, Satyanarayana Tatineni, Siddarame Gowda, Maria A. Ayllon, William O. Dawson

Department of Plant Pathology: Faculty Publications

The long flexuous virions of the Closteroviridae have a unique bipolar architecture incorporating two coat proteins, with most of the helical nucleocapsid encapsidated by the major coat protein (CP) and a small portion of one end encapsidated by the minor coat protein (CPm). It is not known whether CPm encapsidates the genomic RNA and, if so, which end and what effects transition between the two coat proteins. Two other virus-encoded proteins, an HSP70 homolog (HSP70h) and an = 61-kDa protein, are required to augment virion assembly. In this work, we examine the in vivo encapsidation of Citrus tristeza virus by …

Establishing Effects Of Application Of Swine Manure On Crop Leaf Damage To Growing Crops, Charles Shapiro, University Of Nebraska-Lincoln

Department of Agronomy and Horticulture: Faculty Publications

Swine manure from a below barn storage facility was applied to growing corn and soybeans to determine phytotoxic effects. The manure had an electrical conductivity of around 20 mmhos/cm and was diluted for treatment at full, half, quarter and none. The manure mixtures were applied at a rate of 0.5 in/acre using a procedure that wet the foliage three times in a 15-minute time span. Manure mixtures were applied at V7 and V14 for corn and V3 and R1 for soybeans. Phytotoxic effects were shown at the high rate for both crops, but soybeans were killed at the early application …

Comparison Of Modis And Avhrr 16-Day Normalized Difference Vegetation Index Composite Data, Kevin P. Gallo, Lei Ji, Brad Reed, John Dwyer, Jeffrey Eidenshink

School of Natural Resources: Faculty Publications

Normalized difference vegetation index (NDVI) data derived from visible and near-infrared data acquired by the MODIS and AVHRR sensors were compared over the same time periods and a variety of land cover classes within the conterminous USA. The relationship between the AVHRR derived NDVI values and those of future sensors is critical to continued long term monitoring of land surface properties. The results indicate that the 16-day composite values are quite similar over the 23 intervals of 2001 that were analyzed, and a linear relationship exists between the NDVI values from the two sensors. The composite AVHRR NDVI data were …

Seed Quality Qtl In A Prominent Soybean Population, D. L. Hyten Jr., V. R. Pantalone, C. E. Sams, A. M. Saxon, D. Landau-Ellis, T. R. Stefaniak, M. E. Schmidt

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is a versatile crop due to its multitude of uses as a high protein meal and vegetable oil. Soybean seed traits such as seed protein and oil concentration and seed size are important quantitative traits. The objective of this study was to identify representative protein, oil, and seed size quantitative trait loci (QTL) in soybean. A recombinant inbred line (RIL) population consisting of 131 F6-derived lines was created from two prominent ancestors of North American soy-beans (‘Essex’ and ‘Williams’) and the RILs were grown in six environments. One hundred simple sequence repeat (SSR) markers …

Molecular Differentiation Of Two Sibling Species Of The Black Fly Simulium Vittatum (Diptera: Simuliidae) Based On Random Amplified Polymorphic Dna, G. A. Duncan, P. H. Adler, K. P. Pruess, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

Larvae of the black fly morphospecies Simulium vittatum from Colorado, Montana, Nebraska, and New Hampshire were cytologically identified as either the IS-7 or the IIIL-1 cytospecies. DNA was PCR amplified from cytotyped larvae using eight 10-mer primers, labeled with ₃₃P, and run on polyacrylamide gels. The entire data set of 96 amplicons produced incomplete separation of the two cytospecies when subjected to neighbor-joining and maximum parsimony analyses. However, when analyzed within geographical regions, separate species status was supported. Boot- strap support for distinctness of the two cytospecies was best in Colorado where they were collected in true sympatry. The …

The Hopptof Locus Of Pseudomonas Syringae Pv. Tomato Dc3000 Encodes A Type Iii Chaperone And A Cognate Effector, Libo Shan, Hye-Sook Oh, Jianfu Chen, Ming Guo, Jianmin Zhou, James R. Alfano, Alan Collmer, Xu Jia, Xiaoyan Tang

Department of Plant Pathology: Faculty Publications

Type III secretion systems are highly conserved among gram-negative plant and animal pathogenic bacteria. Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells. Analysis of the whole genome sequence of Pseudomonas syringae pv. tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in P. syringae pv. phaseolicola. The HopPtoF locus harbors two genes, ShcF_Pto and HopF_Pto, that are preceded by a single hrp box promoter. We present evidence here to show that ShcF_Pto and HopF_Pto encode a type …

Effects Of Choline, Betaine, And Wheat Floral Extracts On Growth Of Fusarium Graminearum, Jessica S. Engle, Patrick E. Lipps, Terry L. Graham, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Fusarium head blight has been more severe when infection occurs during anthesis, indicating that floral organs may be important infection courts. Choline acetate and glycinebetaine have been extracted from wheat and reported to be growth stimulants of Fusarium graminearum. They are hypothesized to enhance infection and tissue colonization. Growth of F. graminearum was examined on media amended with extracts from floral parts of nine wheat genotypes with various Fusarium head blight resistance levels. Results indicated no significant effect of anther, palea, or lemma extracts on radial growth when compared with unamended controls. Effects on spore germination and hyphal growth …

Nematode Molecular Diagnostics: From Bands To Barcodes, Tom Powers

Department of Plant Pathology: Faculty Publications

Nematodes are considered among the most difficult animals to identify. DNA-based diagnostic methods have already gained acceptance in applications ranging from quarantine determinations to assessments of biodiversity. Researchers are currently in an information-gathering mode, with intensive efforts applied to accumulating nucleotide sequence of 18S and 28S ribosomal genes, internally transcribed spacer regions, and mitochondrial genes. Important linkages with collateral data such as digitized images, video clips and specimen voucher web pages are being established on GenBank and NemATOL, the nematode-specific Tree of Life database. The growing DNA taxonomy of nematodes has lead to their use in testing specific short sequences …

Type Iii Secretion System Effector Proteins: Double Agents In Bacterial Disease And Plant Defense, James R. Alfano, Alan Collmer

Department of Plant Pathology: Faculty Publications

Many phytopathogenic bacteria inject virulence effector proteins into plant cells via a Hrp type III secretion system (TTSS). Without the TTSS, these pathogens cannot defeat basal defenses, grow in plants, produce disease lesions in hosts, or elicit the hypersensitive response (HR) in nonhosts. Pathogen genome projects employing bioinformatic methods to identify TTSS Hrp regulon promoters and TTSS pathway targeting signals suggest that phytopathogenic Pseudomonas, Xanthomonas, and Ralstonia spp. harbor large arsenals of effectors. The Hrp TTSS employs cus- tomized cytoplasmic chaperones, conserved export components in the bacterial en- velope (also used by the TTSS of animal pathogens), and …

Nebline, January 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Rural Living Carries Rewards, Responsibilities
Monthly “Acreage Insights” Clinics Focus on Rural Living Topics
Caring for Florist Azaleas
Garden Guide
Starting Avocado Seeds
Growing Norfolk Island Pines
Prevention and Control of Rabbit Damage
Don’t Let the Bed Bugs Bite!
Birds Need Water in Winter Too
Use Compact Fluorescent Lamps to Save Energy
Crop Protection Clinic Scheduled for Jan. 7
Off-Farm Income Important to Farm Families
Private Pesticide Applicator Training
Sorghum Seminars Scheduled
Use Portable Generators Carefully
Unused and Abandoned Wells
Put In The Big Rocks First!
Touchdown Beef Chili Recipe
10,000 Steps for Health
Serving Soup - Safely!
Tips for …

Using Multi-Spectral Imagery To Evaluate Corn Grown Under Nitrogen And Drought Stressed Conditions, Shannon L. Osborne, James S. Schepers, Mike R. Schlemmer

Department of Agronomy and Horticulture: Faculty Publications

The in-season estimation of crop stresses which have the potential of adversely affecting crop yield and/or quality could allow producers to make in-season management decisions to correct for the particular stress. A field study was conducted to evaluate the use of multispectral imagery for estimating corn (Zea mays L.) grain yield, in-season biomass and nitrogen (N) concentration under varying N and drought stresses. The experiment was a split-plot design with three replications using a factorial arrangement of treatments. Three irrigation (whole-plot) treatments included dry land, irrigation based on 0.5, and full evapotranspiration (ET). Sub-plot treatments included five N …

Expression Of A Putative Flavonoid 3'-Hydroxylase In Sorghum Mesocotyls Synthesizing 3-Deoxyanthocyanidin Phytoalexins, Jayanand Boddu, Catherine Svabek, Rajandeep Sekhon, Amanda Gevens, Ralph L. Nicholson, A. Daniel Jones, Jeffrey F. Pedersen, David L. Gustine, Surinder Chopra

Department of Agronomy and Horticulture: Faculty Publications

In sorghum, ingress of Cochliobolus heterostrophus stimulates the synthesis of 3-deoxyanthocyanidins that act as phytoalexins. Apigeninidin and luteolinidin are two major phytoalexins induced in the first 24 h after infection. In an attempt to understand genetic regulation of the biosynthesis of sorghum phytoalexins, we isolated a differentially expressed partial cDNA. Characterization and comparison showed that this cDNA sequence corresponds to a putative flavonoid 3’-hydroxylase. Full length sequence characterization allowed us to establish that the sorghum putative f3’h cDNA encodes a peptide of 517 amino acids that has domains conserved among cytochrome P450 proteins functioning in …