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Articles 601 - 630 of 1579

Full-Text Articles in Other Plant Sciences

Clarireedia: A New Fungal Genus Comprising Four Pathogenic Species Responsible For Dollar Spot Disease Of Turfgrass, Catalina Salgado-Salazar, Lisa A. Beirn, Adnan Ismaiel, Michael J. Boehm, Ignazio Carbone, Alexander I. Putman, Lane P. Tredway, Bruce B. Clarke, Jo Anne Crouch Apr 2018

Clarireedia: A New Fungal Genus Comprising Four Pathogenic Species Responsible For Dollar Spot Disease Of Turfgrass, Catalina Salgado-Salazar, Lisa A. Beirn, Adnan Ismaiel, Michael J. Boehm, Ignazio Carbone, Alexander I. Putman, Lane P. Tredway, Bruce B. Clarke, Jo Anne Crouch

Department of Plant Pathology: Faculty Publications

Dollar spot is one of the most destructive and economically important fungal diseases of amenity turfgrasses. The causal agent was first described in 1937 as the ascomycete Sclerotinia homoeocarpa. However, the genus-level taxonomic placement of this fungus has been the subject of an ongoing debate for over 75 y. Existing morphological and rDNA sequence evidence indicates that this organism is more appropriately placed in the family Rutstroemiaceae rather than the Sclerotiniaceae. Here we use DNA sequence data from samples of the dollar spot fungus and other members of the Rutstroemiaceae (e.g. Rutstroemia, Lanzia, Lambertella) collected throughout the world to determine …


Impacts Of Cover Crop And Residue Removal On Soil Hydraulic And Thermal Properties, Michael T. Sindelar Apr 2018

Impacts Of Cover Crop And Residue Removal On Soil Hydraulic And Thermal Properties, Michael T. Sindelar

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Large-scale crop residue removal for livestock or biofuel production may negatively affect soil and water resources. A combination of management practices could be the key to manage such resources under increasingly variable climate. For instance, use of cover crops (CCs) could offset the negative impacts that corn (Zea mays L.) residue removal may have on soil water and energy balance. We studied: 1) the effect of corn residue removal (56%) with and without winter rye (Secale cereale L.) CC on soil hydraulic and thermal properties including water infiltration, water retention, pores-size distribution, thermal conductivity, specific heat capacity, and …


Vegetation And Soil Responses To Concrete Grinding Residue Application On Highway Roadsides Of Eastern Nebraska, Ana Wingeyer, Martha Mamo, Walter H. Schacht, Dennis L. Mccallister, Pamela Sutton Apr 2018

Vegetation And Soil Responses To Concrete Grinding Residue Application On Highway Roadsides Of Eastern Nebraska, Ana Wingeyer, Martha Mamo, Walter H. Schacht, Dennis L. Mccallister, Pamela Sutton

Department of Agronomy and Horticulture: Faculty Publications

As a precautionary principle, the National Pollutant Discharge Elimination System (NPDES) permit establishes that the primary pollutant in concrete grinding residue (CGR) is its alkalinity and restricts CGR roadside discharge to 11 Mg ha−1 or the agronomic liming rate, whichever is lower. We evaluated the effect of CGR application on roadside soil chemical properties, existing vegetation, and rainfall runoff. Five CGR rates (0, 11, 22, 45, and 90 dry Mg ha−1) were tested on roadsides slopes at two different locations in eastern Nebraska. Vegetation, soil, and runoff characteristics were evaluated before CGR application and 30 d and …


A Systems Modeling Approach To Forecast Corn Economic Optimum Nitrogen Rate, Laila A. Puntel, John E. Sawyer, Daniel W. Barker, Peter J. Thorburn, Michael J. Castellano, Kenneth J. Moore, Andy Vanloocke, Emily A. Heaton, Sotirios V. Archontoulis Apr 2018

A Systems Modeling Approach To Forecast Corn Economic Optimum Nitrogen Rate, Laila A. Puntel, John E. Sawyer, Daniel W. Barker, Peter J. Thorburn, Michael J. Castellano, Kenneth J. Moore, Andy Vanloocke, Emily A. Heaton, Sotirios V. Archontoulis

Department of Agronomy and Horticulture: Faculty Publications

Historically crop models have been used to evaluate crop yield responses to nitrogen (N) rates after harvest when it is too late for the farmers to make in-season adjustments. We hypothesize that the use of a crop model as an in-season forecast tool will improve current N decision-making. To explore this, we used the Agricultural Production Systems sIMulator (APSIM) calibrated with long-term experimental data for central Iowa, USA (16-years in continuous corn and 15-years in soybean-corn rotation) combined with actual weather data up to a specific crop stage and historical weather data thereafter. The objectives were to: (1) evaluate the …


Review Of Genome-Wide Association Studies: From Polymorphism To Personalized Medicine, Jinliang Yang Mar 2018

Review Of Genome-Wide Association Studies: From Polymorphism To Personalized Medicine, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

Genome-wide association studies (GWAS) has become a powerful tool in the area of quantitative genetics to map the relationship between trait and genomic variations. This volume provides a great resource for beginners to learn about the recent advances in GWAS and for domain experts to identify the gaps in the area. The first part of the volume lays out the statistical background of GWAS. I really liked the article by Yang et al., Introduction to Statistical Methods in Genome-Wide Association Studies. In this chapter, the authors talked about the missing heritability issue and introduced ways to calculate heritability using the …


An Epigenetic Breeding System In Soybean For Increased Yield And Stability, Sunil K. Kenchanmane Raju, Mon-Ray Shao, Robersy Sanchez, Ying-Zhi Xu, Ajay Sandhu, George L. Graef, Sally A. Mackenzie Feb 2018

An Epigenetic Breeding System In Soybean For Increased Yield And Stability, Sunil K. Kenchanmane Raju, Mon-Ray Shao, Robersy Sanchez, Ying-Zhi Xu, Ajay Sandhu, George L. Graef, Sally A. Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Epigenetic variation has been associated with a wide range of adaptive phenotypes in plants, but there exist few direct means for exploiting this variation. RNAi suppression of the plant-specific gene, MutS HOMOLOG1 (MSH1), in multiple plant species produces a range of developmental changes accompanied by modulation of defence, phytohormone and abiotic stress response pathways along with methylome repatterning. This msh1-conditioned developmental reprogramming is retained independent of transgene segregation, giving rise to transgene-null ‘memory’ effects. An isogenic memory line crossed to wild type produces progeny families displaying increased variation in adaptive traits that respond to selection. This study …


Inheritance Of Mesotrione Resistance In An Amaranthus Tuberculatus (Var. Rudis) Population From Nebraska, Usa, Maxwel C. Oliveira, Todd A. Gaines, Amit J. Jhala, Stevan Z. Knezevic Feb 2018

Inheritance Of Mesotrione Resistance In An Amaranthus Tuberculatus (Var. Rudis) Population From Nebraska, Usa, Maxwel C. Oliveira, Todd A. Gaines, Amit J. Jhala, Stevan Z. Knezevic

Department of Agronomy and Horticulture: Faculty Publications

A population of Amaranthus tuberculatus (var. rudis) evolved resistance to 4- hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicides (mesotrione, tembotrione, and topramezone) in Nebraska. The level of resistance was the highest to mesotrione, and the mechanism of resistance in this population is metabolism-based likely via cytochrome P450 enzymes. The increasing number of weeds resistant to herbicides warrants studies on the ecology and evolutionary factors contributing for resistance evolution, including inheritance of resistance traits. In this study, we investigated the genetic control of mesotrione resistance in an A. tuberculatus population from Nebraska, USA. Results showed that reciprocal crosses in the F1 families exhibited …


Genome-Wide Analysis Of Grain Yield Stability And Environmental Interactions In A Multiparental Soybean Population, Alencar Xavier, Diego Jarquin, Reka Howard, Vishnu Ramasubramanian, James E. Specht, George L. Graef, William D. Beavis, Brian W. Diers, Qijian Song, Perry B. Cregan, Randall L. Nelson, Rouf Mian, J. Grover Shannon, Leah K. Mchale, Dechun Wang, William Schapaugh, Aaron J. Lorenz, Shizhong Xu, William M. Muir, Katy M. Rainey Feb 2018

Genome-Wide Analysis Of Grain Yield Stability And Environmental Interactions In A Multiparental Soybean Population, Alencar Xavier, Diego Jarquin, Reka Howard, Vishnu Ramasubramanian, James E. Specht, George L. Graef, William D. Beavis, Brian W. Diers, Qijian Song, Perry B. Cregan, Randall L. Nelson, Rouf Mian, J. Grover Shannon, Leah K. Mchale, Dechun Wang, William Schapaugh, Aaron J. Lorenz, Shizhong Xu, William M. Muir, Katy M. Rainey

Department of Agronomy and Horticulture: Faculty Publications

Genetic improvement toward optimized and stable agronomic performance of soybean genotypes is desirable for food security. Understanding how genotypes perform in different environmental conditions helps breeders develop sustainable cultivars adapted to target regions. Complex traits of importance are known to be controlled by a large number of genomic regions with small effects whose magnitude and direction are modulated by environmental factors. Knowledge of the constraints and undesirable effects resulting from genotype by environmental interactions is a key objective in improving selection procedures in soybean breeding programs. In this study, the genetic basis of soybean grain yield responsiveness to environmental factors …


Overlapping Residual Herbicides For Control Of Photosystem (Ps) Ii- And 4-Hydroxyphenylpyruvate Dioxygenase (Hppd)-Inhibitor-Resistant Palmer Amaranth (Amaranthus Palmeri S. Watson) In Glyphosate-Resistant Maize, Parminder S. Chahal, Zahoor Ahmad Ganie, Amit J. Jhala Jan 2018

Overlapping Residual Herbicides For Control Of Photosystem (Ps) Ii- And 4-Hydroxyphenylpyruvate Dioxygenase (Hppd)-Inhibitor-Resistant Palmer Amaranth (Amaranthus Palmeri S. Watson) In Glyphosate-Resistant Maize, Parminder S. Chahal, Zahoor Ahmad Ganie, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

A Palmer amaranth (Amaranthus palmeri S. Watson) biotype has evolved resistance to photosystem (PS) II- (atrazine) and 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides (mesotrione, tembotrione, and topramezone) in maize seed production field in Nebraska, USA. The objectives of this study were to determine the effect of soil residual pre-emergence (PRE) herbicides followed by (fb) tank-mixture of residual and foliar active post-emergence (POST) herbicides on PS-II- and HPPD-inhibitor-resistant Palmer amaranth control, maize yield, and net economic returns. Field experiments were conducted in a grower’s field infested with PS II- and HPPD-inhibitor-resistant Palmer amaranth near Shickley in Fillmore County, Nebraska, USA in 2015 …


Evaluation Of Bacillus Strains For Plant Growth Promotion And Predictability Of Efficacy By In Vitro Physiological Traits, Rufus J. Akinrinlola, Gary Y. Yuen, Rhae A. Drijber, Anthony O. Adesemoye Jan 2018

Evaluation Of Bacillus Strains For Plant Growth Promotion And Predictability Of Efficacy By In Vitro Physiological Traits, Rufus J. Akinrinlola, Gary Y. Yuen, Rhae A. Drijber, Anthony O. Adesemoye

Department of Plant Pathology: Faculty Publications

Bacilli are commonly used as plant growth-promoting agents but can be limited in effectiveness to certain crop and soil environments. The objectives of this study were to (1) identify Bacillus strains that can be consistent in promoting the growth of corn, wheat, and soybean and (2) determine whether physiological traits expressed in vitro can be predictive of growth promotion efficacy/consistency and be used for selecting effective strains. Twelve Bacillus strains isolated from wheat rhizospheres were evaluated in greenhouse pot tests with nonsterile soil for their effects on the growth of corn, soybean, and wheat. The strains also were assessed in …


Field Evaluation Of Commercially Available Small Unmanned Aircraft Crop Spray Systems, Wayne Woldt, D. Martin, M. Latheef, Greg Kruger, Robert J. Wright, Justin Mcmechan, Christopher A. Proctor, Tamra Jackson-Ziems Jan 2018

Field Evaluation Of Commercially Available Small Unmanned Aircraft Crop Spray Systems, Wayne Woldt, D. Martin, M. Latheef, Greg Kruger, Robert J. Wright, Justin Mcmechan, Christopher A. Proctor, Tamra Jackson-Ziems

Department of Plant Pathology: Faculty Publications

Agricultural research and development on small unmanned aircraft systems (UAS) has been directed toward UAS enabled sensing to detect features of interest. While compelling, there is an immediate need to increase the breadth and depth of UAS-based research, to move beyond sensing, and explore active intervention in agricultural production systems. This paper is focused on the concept of crop protection through ultra-precise, unmanned aerial application systems, and seeks to initiate research discussion in this important area of opportunity. Toward this end, two different, commercially available, small Unmanned Aerial Application Systems (sUAAS - defined as less than 55 lbs. maximum take-off …


Optimising The Identification Of Causal Variants Across Varying Genetic Architectures In Crops, Chenyong Miao, Jinliang Yang, James C. Schnable Jan 2018

Optimising The Identification Of Causal Variants Across Varying Genetic Architectures In Crops, Chenyong Miao, Jinliang Yang, James C. Schnable

Department of Agronomy and Horticulture: Faculty Publications

Association studies use statistical links between genetic markers and the phenotype variation across many individuals to identify genes controlling variation in the target phenotype. However, this approach, particularly conducted on a genome-wide scale (GWAS), has limited power to identify the genes responsible for variation in traits controlled by complex genetic architectures. In this study, we employ real-world genotype datasets from four crop species with distinct minor allele frequency distributions, population structures and linkage disequilibrium patterns. We demonstrate that different GWAS statistical approaches provide favourable trade-offs between power and accuracy for traits controlled by different types of genetic architectures. FarmCPU provides …


Transcriptional Reprogramming Of Legume Genomes: Perspective And Challenges Associated With Single-Cell And Single Cell-Type Approaches During Nodule Development, Marc Libault Jan 2018

Transcriptional Reprogramming Of Legume Genomes: Perspective And Challenges Associated With Single-Cell And Single Cell-Type Approaches During Nodule Development, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Transcriptomic approaches revealed thousands of genes differentially or specifically expressed during nodulation, a biological process resulting from the symbiosis between leguminous plant roots and rhizobia, atmospheric nitrogen-fixing symbiotic bacteria. Ultimately, nodulation will lead to the development of a new root organ, the nodule. Through functional genomic studies, plant transcriptomes have been used by scientists to reveal plant genes potentially controlling nodulation. However, it is important to acknowledge that the physiology, transcriptomic programs, and biochemical properties of the plant cells involved in nodulation are continuously regulated. They also differ between the different cell-types composing the nodules. To generate a more accurate …


First Case Of Glufosinate-Resistant Rigid Ryegrass (Lolium Rigidum Gaud.) In Greece, Ilias S. Travlos, Nikolina Cheimona, Rafael De Prado, Amit J. Jhala, Demosthenis Chachalis, Eleni Tani Jan 2018

First Case Of Glufosinate-Resistant Rigid Ryegrass (Lolium Rigidum Gaud.) In Greece, Ilias S. Travlos, Nikolina Cheimona, Rafael De Prado, Amit J. Jhala, Demosthenis Chachalis, Eleni Tani

Department of Agronomy and Horticulture: Faculty Publications

Repeated applications of the same herbicide(s), which are characterized by the same mode of action, increase selection pressure, which in turn favours the evolution of herbicide-resistant weeds. Glufosinate is a broad-spectrum non-selective herbicide being used for weed control for many years around the world. Rigid ryegrass (Lolium rigidum Gaud.) is an economically important grass weed in Greece. Recent complaints by growers about control failure of rigid ryegrass with glufosinate require further investigation and have been the basis of this study. The objectives of this study were to confirm the existence of glufosinate-resistant L. rigidum in Greece and evaluate the …


Empowerment And Tech Adoption: Introducing The Treadle Pump Triggers Farmers’ Innovation In Eastern Ethiopia, Shimelis Beyene, Teshome H. Regassa, Belaineh Legesse, Martha Mamo, Tsegaye Tadesse Jan 2018

Empowerment And Tech Adoption: Introducing The Treadle Pump Triggers Farmers’ Innovation In Eastern Ethiopia, Shimelis Beyene, Teshome H. Regassa, Belaineh Legesse, Martha Mamo, Tsegaye Tadesse

Department of Agronomy and Horticulture: Faculty Publications

In 2013, thirty-eight treadle pumps (TPs) were installed as low-cost technology introduction for small-scale irrigation in eastern Ethiopia. This pilot project also trained six farmers on tube well excavation, as well as the installation and maintenance of pumps. In June 2015, researchers visited nine of the thirty-eight TP villages and found only two TPs functioning as originally installed. The rest were replaced with a new technology developed by the trained farmers. Farmers, empowered by training, gained more control in developing technology options tailored to local needs and conditions of their communities. Adopters of the new technology stated that the limited …


Beyond The Plot: Technology Extrapolation Domains For Scaling Out Agronomic Science, Juan I. Rattalino Edreira, Kenneth G. Cassman, Zvi Hochman, Martin K. Van Ittersum, Lenny Van Bussel, Lieven Claessens, Patricio Grassini Jan 2018

Beyond The Plot: Technology Extrapolation Domains For Scaling Out Agronomic Science, Juan I. Rattalino Edreira, Kenneth G. Cassman, Zvi Hochman, Martin K. Van Ittersum, Lenny Van Bussel, Lieven Claessens, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Ensuring an adequate food supply in systems that protect environmental quality and conserve natural resources requires productive and resource-efficient cropping systems on existing farmland.Meeting this challenge will be difficult without a robust spatial framework that facilitates rapid evaluation and scaling-out of currently available and emerging technologies. Here we develop a global spatial framework to delineate ‘technology extrapolation domains’ based on key climate and soil factors that govern crop yields and yield stability in rainfed crop production. The proposed framework adequately represents the spatial pattern of crop yields and stability when evaluated over the data-rich US Corn Belt. It also facilitates …


Is Conservation Agriculture A Potential Option For Cereal-Based Sustainable Farming System In The Eastern Indo-Gangetic Plains Of Nepal?, Dipendra Pokharel, Raj Kant Jha, Thakur Prasad Tiwari, Mahesh Kumar Gathala, Hari Krishna Shrestha, Dinesh Panday Jan 2018

Is Conservation Agriculture A Potential Option For Cereal-Based Sustainable Farming System In The Eastern Indo-Gangetic Plains Of Nepal?, Dipendra Pokharel, Raj Kant Jha, Thakur Prasad Tiwari, Mahesh Kumar Gathala, Hari Krishna Shrestha, Dinesh Panday

Department of Agronomy and Horticulture: Faculty Publications

A decline in land and water productivity, increase in the cost of cultivation, and labor-intensive practices are affecting the cereal-based farming system in Nepal, particularly in the Indo-Gangetic Plains (IGP). Conservation agriculture (CA) practices have been found to be the climate-, energy-, and labor-smart and sustainable agricultural production technologies. Sustainable and Resilient Farming System Intensification (SRFSI) has been working since 2014 in response to the sustainability of the cereal-based (rice–wheat and rice–maize) farming in Sunsari and Dhanusha districts of Nepal. This study was conducted to assess the adoption and scaling up of CA in addition to input usage, production, net …


Impact Of Drought On Chemical Composition And Sugar Yields From Dilute-Acid Pretreatment And Enzymatic Hydrolysis Of Miscanthus, A Tall Fescue Mixture, And Switchgrass, Amber Hoover, Rachel Emerson, Allison Ray, Daniel Stevens, Sabrina Morgan, Marnie Cortez, Robert Kallenbach, Matthew Sousek, Rodney Farris, Dayna Daubaras Jan 2018

Impact Of Drought On Chemical Composition And Sugar Yields From Dilute-Acid Pretreatment And Enzymatic Hydrolysis Of Miscanthus, A Tall Fescue Mixture, And Switchgrass, Amber Hoover, Rachel Emerson, Allison Ray, Daniel Stevens, Sabrina Morgan, Marnie Cortez, Robert Kallenbach, Matthew Sousek, Rodney Farris, Dayna Daubaras

Department of Agronomy and Horticulture: Faculty Publications

Environmental factors like drought impact the quality of biomass entering a bioconversion process. Drought often reduces the sugar content in lignocellulosic biomass, which could have economic impacts, particularly when compounded with losses in dry biomass yield; however, the effects on conversion efficiency are not completely understood. This study investigated how drought may impact biomass composition and sugar yields from dilute-acid pretreatment and enzymatic hydrolysis of Miscanthus, a tall fescue mixture, and switchgrass from Nebraska, Missouri, and Oklahoma, respectively, grown as part of Regional Feedstock Partnership field trials. Samples were grown and harvested in 2010 during non-drought conditions and in …


Impaired Phloem Loading In Zmsweet13a,B,C Sucrose Transporter Triple Knock-Out Mutants In Zea Mays, Margaret Bezrutczyk, Thomas Hartwig, Marc Horschman, Si Nian Char, Jinliang Yang, Bing Yang, Wolf B. Frommer, Davide Sosso Jan 2018

Impaired Phloem Loading In Zmsweet13a,B,C Sucrose Transporter Triple Knock-Out Mutants In Zea Mays, Margaret Bezrutczyk, Thomas Hartwig, Marc Horschman, Si Nian Char, Jinliang Yang, Bing Yang, Wolf B. Frommer, Davide Sosso

Department of Agronomy and Horticulture: Faculty Publications

Crop yield is critical for human nutrition, yet the underlying machinery that ultimately determines yield potential is still not understood. Crop productivity under ideal conditions is determined by the efficiency with which plants intercept light, convert it into chemical energy, translocate photosynthates and convert these to storage products in harvestable organs (Zhu et al., 2010). In many crops, sucrose is the primary form for translocation inside the conduit (i.e. the phloem). A combination of SWEETmediated efflux from phloem parenchyma and subsequent secondary active sucrose import by SUT sucrose/H+ symporters is thought to create the driving force for pressure gradient-driven …


Tetracycline And Sulfonamide Antibiotic Resistance Genes In Soils From Nebraska Organic Farming Operations, Marylynn Cadena, Lisa M. Durso, Daniel N. Miller, Heidi M. Waldrip, B. L. Castleberry, Rhae A. Drijber, Charles S. Wortmann Jan 2018

Tetracycline And Sulfonamide Antibiotic Resistance Genes In Soils From Nebraska Organic Farming Operations, Marylynn Cadena, Lisa M. Durso, Daniel N. Miller, Heidi M. Waldrip, B. L. Castleberry, Rhae A. Drijber, Charles S. Wortmann

Department of Agronomy and Horticulture: Faculty Publications

There is widespread agreement that agricultural antibiotic resistance should be reduced, however, it is unclear from the available literature what an appropriate target for reduction would be. Organic farms provide a unique opportunity to disentangle questions of agricultural antibiotic drug use from questions of antibiotic resistance in the soil. In this study, soil was collected from 12 certified organic farms in Nebraska, evaluated for the presence of tetracycline and sulfonamide resistance genes (n = 15 targets), and correlated to soil physical, chemical, and biological parameters. Tetracycline and sulfonamide antibiotic resistance genes (ARGs) were found in soils from all 12 …


Sifting And Winnowing: Analysis Of Farmer Field Data For Soybean In The Us North-Central Region, Spyridon Mourtzinis, Juan I. Rattalino Edreira, Patricio Grassini, Adam C. Roth, Shaun N. Casteel, Ignacio A. Ciampitti, Hans J. Kandel, Peter M. Kyveryga, Mark A. Licht, Laura E. Lindsey, Daren S. Mueller, Emerson D. Nafziger, Seth L. Naeve, Jordan Stanley, Michael J. Staton, Shawn P. Conley Jan 2018

Sifting And Winnowing: Analysis Of Farmer Field Data For Soybean In The Us North-Central Region, Spyridon Mourtzinis, Juan I. Rattalino Edreira, Patricio Grassini, Adam C. Roth, Shaun N. Casteel, Ignacio A. Ciampitti, Hans J. Kandel, Peter M. Kyveryga, Mark A. Licht, Laura E. Lindsey, Daren S. Mueller, Emerson D. Nafziger, Seth L. Naeve, Jordan Stanley, Michael J. Staton, Shawn P. Conley

Department of Agronomy and Horticulture: Faculty Publications

Field trials are commonly used to estimate the effects of different factors on crop yields. In the present study, we followed an alternative approach to identify factors that explain field-to-field yield variation, which consisted of farmer survey data, a spatial framework, and multiple statistical procedures. This approach was used to identify management factors with strongest association with on-farm soybean yield variation in the US North Central (NC) region. Field survey data, including yield and management information, were collected over two crop growing seasons (2014 and 2015) from rainfed and irrigated soybean fields (total of 3568 field-year observations). Fields were grouped …


Characterization Of Neofabraea Actinidiae And N. Brasiliensis As Causal Agents Of Apple Bull’S-Eye Rot In Southern Brazil, Amauri Bogo, Carla C. Comparin, Rosa M. Valdebenito Sanhueza, Patricia Ritschel, Ricardo T. Casa, Fábio N. Silva, Sydney E. Everhart Jan 2018

Characterization Of Neofabraea Actinidiae And N. Brasiliensis As Causal Agents Of Apple Bull’S-Eye Rot In Southern Brazil, Amauri Bogo, Carla C. Comparin, Rosa M. Valdebenito Sanhueza, Patricia Ritschel, Ricardo T. Casa, Fábio N. Silva, Sydney E. Everhart

Department of Plant Pathology: Faculty Publications

The causal agents of apple bull’s-eye rot in southern Brazil have recently been described as Neofabraea actinidiae and N. brasiliensis. Isolates of both species were evaluated for response of mycelial growth index (MGI) to different temperatures, enzyme production, mycelial growth inhibition and effective concentrations (EC50 and EC100) of the fungicides triflumizole, pyrimethanil and thiophanate methyl, as well as aggressiveness on fruits of ‘Fuji’ hybrid and ‘Pink Lady’. There was significantly lower mycelium growth in N. brasiliensis compared with N. actinidiae at all temperatures tested. Neither species grew at 3 and 32°C. There were minor differences in …


Elucidating The Role Of Highly Homologous Nicotiana Benthamiana Ubiquitin E2 Gene Family Members In Plant Immunity Through An Improved Virus‑Induced Gene Silencing Approach, Bangjun Zhou, Lirong Zeng Jan 2018

Elucidating The Role Of Highly Homologous Nicotiana Benthamiana Ubiquitin E2 Gene Family Members In Plant Immunity Through An Improved Virus‑Induced Gene Silencing Approach, Bangjun Zhou, Lirong Zeng

Department of Plant Pathology: Faculty Publications

Background: Virus-induced gene silencing (VIGS) has been used in many plant species as an attractive post transcriptional gene silencing (PTGS) method for studying gene function either individually or at large-scale in a high-throughput manner. However, the specificity and efficiency for knocking down members of a highly homologous gene family have remained to date a significant challenge in VIGS due to silencing of off-targets. Results: Here we present an improved method for the selection and evaluation of gene fragments used for VIGS to specifically and efficiently knock down members of a highly homologous gene family. Using this method, we knocked down …


The Tomato U-Box Type E3 Ligase Pub13 Acts With Group Iii Ubiquitin E2 Enzymes To Modulate Fls2-Mediated Immune Signaling, Bangjun Zhou, Lirong Zeng Jan 2018

The Tomato U-Box Type E3 Ligase Pub13 Acts With Group Iii Ubiquitin E2 Enzymes To Modulate Fls2-Mediated Immune Signaling, Bangjun Zhou, Lirong Zeng

Department of Plant Pathology: Faculty Publications

In Arabidopsis and rice, the ubiquitin ligase PUB13-mediated protein degradation plays a significant role in plant pattern-triggered immunity (PTI) and flowering time control. The Arabidopsis PUB13 has been shown to attenuate the pattern recognition receptor FLS2-mediated immune signaling by ubiquitinating FLS2 and consequently promoting its degradation by the 26S proteasome. Nevertheless, the cognate ubiquitin-conjugating enzymes (E2) with which PUB13 acts to modulate FLS2-mediated PTI are unknown. To address this question, we investigate here the tomato (Solanum lycopersicum) homolog of PUB13, SlPUB13 by utilizing the recently characterized complete set of tomato E2s. Of the 13 groups of tomato E2s, …


Proteomic Analysis Of Ubiquitinated Proteins In Rice (Oryza Sativa) After Treatment With Pathogen-Associated Molecular Pattern (Pamp) Elicitors, Xiao-Lin Chen, Xin Xie, Liye Wu, Caiyun Liu, Lirong Zeng, Xueping Zhou, Feng Luo, Guo-Liang Wang, Wende Liu Jan 2018

Proteomic Analysis Of Ubiquitinated Proteins In Rice (Oryza Sativa) After Treatment With Pathogen-Associated Molecular Pattern (Pamp) Elicitors, Xiao-Lin Chen, Xin Xie, Liye Wu, Caiyun Liu, Lirong Zeng, Xueping Zhou, Feng Luo, Guo-Liang Wang, Wende Liu

Department of Plant Pathology: Faculty Publications

Reversible protein ubiquitination plays essential roles in regulating cellular processes. Although many reports have described the functions of ubiquitination in plant defense responses, few have focused on global changes in the ubiquitome. To better understand the regulatory roles of ubiquitination in rice pattern-triggered immunity (PTI), we investigated the ubiquitome of rice seedlings after treatment with two pathogen-associated molecular patterns, the fungal-derived chitin or the bacterialderived flg22, using label-free quantitative proteomics. In chitin-treated samples, 144 and 167 lysine-ubiquitination sites in 121 and 162 proteins showed increased and decreased ubiquitination, respectively. In flg22-treated samples, 151 and 179 lysine-ubiquitination sites in 118 and …


Application Of Machine Learning Methodologies For Predicting Corn Economic Optimal Nitrogen Rate, Zhisheng Qin, D. Brenton Myers, Curtis J. Ransom, Newell R. Kitchen, Sang-Zi Liang, James J. Camberato, Paul R. Carter, Richard B. Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A. M. Laboski, Brad D. Malone, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan Jan 2018

Application Of Machine Learning Methodologies For Predicting Corn Economic Optimal Nitrogen Rate, Zhisheng Qin, D. Brenton Myers, Curtis J. Ransom, Newell R. Kitchen, Sang-Zi Liang, James J. Camberato, Paul R. Carter, Richard B. Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A. M. Laboski, Brad D. Malone, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Determination of in-season N requirement for corn (Zea mays L.) is challenging due to interactions of genotype, environment, and management. Machine learning (ML), with its predictive power to tackle complex systems, may solve this barrier in the development of locally based N recommendations. The objective of this study was to explore application of ML methodologies to predict economic optimum nitrogen rate (EONR) for corn using data from 47 experiments across the US Corn Belt. Two features, a water table adjusted available water capacity (AWCwt) and a ratio of in-season rainfall to AWCwt (RAWCwt), were …


Managing Wicked Herbicide-Resistance: Lessons From The Field, Jill Schroeder, Michael Barrett, David R. Shaw, Amy B. Asmus, Harold Coble, David Ervin, Raymond A. Jussaume Jr., Michael D. K. Owen, Ian Burke, Cody F. Creech, A. Stanley Culpepper, William S. Curran, Darrin M. Dodds, Todd A. Gaines, Jeffrey L. Gunsolus, Bradley D. Hanson, Prashant Jha, Annie E. Klodd, Andrew R. Kniss, Ramon G. Leon, Sandra Mcdonald, Don W. Morishita, Brian J. Schutte, Christy L. Sprague, Phillip W. Stahlman, Larry E. Steckel, Mark J. Vangessel Jan 2018

Managing Wicked Herbicide-Resistance: Lessons From The Field, Jill Schroeder, Michael Barrett, David R. Shaw, Amy B. Asmus, Harold Coble, David Ervin, Raymond A. Jussaume Jr., Michael D. K. Owen, Ian Burke, Cody F. Creech, A. Stanley Culpepper, William S. Curran, Darrin M. Dodds, Todd A. Gaines, Jeffrey L. Gunsolus, Bradley D. Hanson, Prashant Jha, Annie E. Klodd, Andrew R. Kniss, Ramon G. Leon, Sandra Mcdonald, Don W. Morishita, Brian J. Schutte, Christy L. Sprague, Phillip W. Stahlman, Larry E. Steckel, Mark J. Vangessel

Department of Agronomy and Horticulture: Faculty Publications

Herbicide resistance is ‘wicked’ in nature; therefore, results of the many educational efforts to encourage diversification of weed control practices in the United States have been mixed. It is clear that we do not sufficiently understand the totality of the grassroots obstacles, concerns, challenges, and specific solutions needed for varied crop production systems. Weed management issues and solutions vary with such variables as management styles, regions, cropping systems, and available or affordable technologies. Therefore, to help the weed science community better understand the needs and ideas of those directly dealing with herbicide resistance, seven half-day regional listening sessions were held …


Growth, Fitness, And Overwinter Survival Of A Shattercane (Sorghum Bicolor Ssp. Drummondii) × Grain Sorghum (Sorghum Bicolor Ssp. Bicolor) F2 Population, Jared J. Schmidt, Melinda K. Yerka, Jeffrey F. Pedersen, John L. Lindquist Jan 2018

Growth, Fitness, And Overwinter Survival Of A Shattercane (Sorghum Bicolor Ssp. Drummondii) × Grain Sorghum (Sorghum Bicolor Ssp. Bicolor) F2 Population, Jared J. Schmidt, Melinda K. Yerka, Jeffrey F. Pedersen, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Although sorghum [Sorghum bicolor (L.) Moench ssp. bicolor] is the fifth most important grain crop in terms of global production, no commercial hybrids carry genetically engineered (GE) traits for resistance to insect pests or herbicides due to regulatory concerns about gene flow to weedy relatives. However, non-GE herbicide resistance currently is being developed in grain sorghum and will likely transfer to related weeds. Monitoring the impact of this new nuclear technology on the evolution and invasiveness of related weeds requires a baseline understanding of the population biology of grain sorghum genes once they transfer to in situ weed …


Phenotypic Data From Inbred Parents Can Improve Genomic Prediction In Pearl Millet Hybrids, Zhikai Liang, Shashi K. Gupta, Cheng-Ting Yeh, Yang Zhang, Daniel W. Ngu, Ramesh Kumar, Hemant T. Patil, Kanulal D. Mungra, Dev Vart Yadav, Abhishek Rathore, Rakesh K. Srivastava, Rajeev Gupta, Jinliang Yang, Rajeev K. Varshney, Patrick S. Schnable, James C. Schnable Jan 2018

Phenotypic Data From Inbred Parents Can Improve Genomic Prediction In Pearl Millet Hybrids, Zhikai Liang, Shashi K. Gupta, Cheng-Ting Yeh, Yang Zhang, Daniel W. Ngu, Ramesh Kumar, Hemant T. Patil, Kanulal D. Mungra, Dev Vart Yadav, Abhishek Rathore, Rakesh K. Srivastava, Rajeev Gupta, Jinliang Yang, Rajeev K. Varshney, Patrick S. Schnable, James C. Schnable

Department of Agronomy and Horticulture: Faculty Publications

Pearl millet is a non-model grain and fodder crop adapted to extremely hot and dry environments globally. In India, a great deal of public and private sectors’ investment has focused on developing pearl millet single cross hybrids based on the cytoplasmic-genetic male sterility (CMS) system, while in Africa most pearl millet production relies on open pollinated varieties. Pearl millet lines were phenotyped for both the inbred parents and hybrids stage. Many breeding efforts focus on phenotypic selection of inbred parents to generate improved parental lines and hybrids. This study evaluated two genotyping techniques and four genomic selection schemes in pearl …


Isolation And Analysis Of Microbial Communities In Soil, Rhizosphere, And Roots In Perennial Grass Experiments, Morgan R. Mcpherson, Peng Wang, Robert B. Mitchell, Daniel P. Schachtman Jan 2018

Isolation And Analysis Of Microbial Communities In Soil, Rhizosphere, And Roots In Perennial Grass Experiments, Morgan R. Mcpherson, Peng Wang, Robert B. Mitchell, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Plant and soil microbiome studies are becoming increasingly important for understanding the roles microorganisms play in agricultural productivity. The purpose of this manuscript is to provide detail on how to rapidly sample soil, rhizosphere, and endosphere of replicated field trials and analyze changes that may occur in the microbial communities due to sample type, treatment, and plant genotype. The experiment used to demonstrate these methods consists of replicated field plots containing two, pure, warm-season grasses (Panicum virgatum and Andropogon gerardii) and a low-diversity grass mixture (A. gerardii, Sorghastrum nutans, and Bouteloua curtipendula). Briefly, plants …