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Articles 1351 - 1380 of 1579

Full-Text Articles in Other Plant Sciences

Fertility Restoration Of The Sorghum A3 Male-Sterile Cytoplasm Through A Sporophytic Mechanism Derived From Sudangrass, Hoang V. Tang, Jeffrey F. Pedersen, Christine D. Chase, Daryl R. Pring Jan 2007

Fertility Restoration Of The Sorghum A3 Male-Sterile Cytoplasm Through A Sporophytic Mechanism Derived From Sudangrass, Hoang V. Tang, Jeffrey F. Pedersen, Christine D. Chase, Daryl R. Pring

Department of Agronomy and Horticulture: Faculty Publications

Fertility restoration of sorghum [Sorghum bicolor (L.) Moench] lines carrying the IS1112C (A3 group) male-sterile cytoplasm has been documented as a two-gene gametophytic mechanism involving complementary action of restoring alleles designated Rf3 and Rf4, as derived from IS1112C. Fertility restoration capability has also been reported from sudangrass (S. bicolor subsp. drummondii) populations. We describe characteristics of a fertility restoration system derived from sudangrass, in which male-sterile individuals were observed at high frequency in backcross and F2-F3 segregating populations. Segregation analyses were consistent with a sporophytic restoration system involving two complementary genes. Pollen iodine …


Publishing Ltd Whole-Plant Mineral Partitioning Throughout The Life Cycle In Arabidopsis Thaliana Ecotypes Columbia, Landsberg Erecta, Cape Verde Islands, And The Mutant Line Ysl1ysl3, Brian M. Waters, Michael A. Grusak Jan 2007

Publishing Ltd Whole-Plant Mineral Partitioning Throughout The Life Cycle In Arabidopsis Thaliana Ecotypes Columbia, Landsberg Erecta, Cape Verde Islands, And The Mutant Line Ysl1ysl3, Brian M. Waters, Michael A. Grusak

Department of Agronomy and Horticulture: Faculty Publications

• Minimal information exists on whole-plant dynamics of mineral flow through Arabidopsis thaliana or on the source tissues responsible for mineral export to developing seeds. Understanding these phenomena in a model plant could help in the development of nutritionally enhanced crop cultivars. • A whole-plant partitioning study, using sequential harvests, was conducted to characterize growth and mineral concentrations and contents of rosettes, cauline leaves, stems, immature fruit, mature fruit hulls, and seeds of three WT lines (Col-0, Ler, and Cvi) and one mutant line (Col-0::ysl1ysl3). • Shoot mineral content increased throughout the life cycle for all minerals, although tissue-specific mineral …


A Soybean Transcript Map: Gene Distribution, Haplotype And Single-Nucleotide Polymorphism Analysis, Ik-Young Choi, D. L. Hyten, Lakshmi K. Matukumalli, Qijian Song, Julian M. Chaky, Charles V. Quigley, Kevin Chase, K. Gordon Lark, Robert S. Reiter, Mun-Sup Yoon, Eun-Young Hwang, Seung-In Yi, Nevin D. Young, Randy C. Shoemaker, Curtis P. Van Tassell, James E. Specht, P. B. Cregan Jan 2007

A Soybean Transcript Map: Gene Distribution, Haplotype And Single-Nucleotide Polymorphism Analysis, Ik-Young Choi, D. L. Hyten, Lakshmi K. Matukumalli, Qijian Song, Julian M. Chaky, Charles V. Quigley, Kevin Chase, K. Gordon Lark, Robert S. Reiter, Mun-Sup Yoon, Eun-Young Hwang, Seung-In Yi, Nevin D. Young, Randy C. Shoemaker, Curtis P. Van Tassell, James E. Specht, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

The first genetic transcript map of the soybean genome was created by mapping one SNP in each of 1141 genes in one or more of three recombinant inbred line mapping populations, thus providing a picture of the distribution of genic sequences across the mapped portion of the genome. Singlenucleotide polymorphisms (SNPs) were discovered via the resequencing of sequence-tagged sites (STSs) developed from expressed sequence tag (EST) sequence. From an initial set of 9459 polymerase chain reaction primer sets designed to a diverse set of genes, 4240 STSs were amplified and sequenced in each of six diverse soybean genotypes. In the …


Genetic Diversity In Populations Of Xanthomonas Campestris Pv. Campestris In Cruciferous Weeds In Central Coastal California, A. Ignatov, A. Sechler, E. L. Schuenzel, I. Agarkova, B. Oliver, A. K. Vidaver, N. W. Schaad Jan 2007

Genetic Diversity In Populations Of Xanthomonas Campestris Pv. Campestris In Cruciferous Weeds In Central Coastal California, A. Ignatov, A. Sechler, E. L. Schuenzel, I. Agarkova, B. Oliver, A. K. Vidaver, N. W. Schaad

Department of Plant Pathology: Faculty Publications

Xanthomonas campestris pv. campestris (X. campestris) infects a large number of cruciferous plants, including weeds. California has one of the largest and most diverse populations of wild cruciferous plants in the world. Although considerable information is available on the genetic diversity of X. campestris in commercial crop plants, nothing is known about the diversity in strains infecting weeds. To assess the genetic diversity among strains of X. campestris in weeds in noncultivated and cultivated areas, strains of the pathogen were isolated from populations of cruciferous weeds growing in coastal valley crop-production sites and from remote nonproduction sites along the …


Expression Analysis Of Rice Defense-Related Genes In Turfgrass In Response To Magnaporthe Grisea, Young-Ki Jo, Guo-Liang Wang, Michael J. Boehm Jan 2007

Expression Analysis Of Rice Defense-Related Genes In Turfgrass In Response To Magnaporthe Grisea, Young-Ki Jo, Guo-Liang Wang, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Magnaporthe grisea (anamorph = Pyricularia grisea) causes blast on rice (Oryza sativa) and gray leaf spot on turfgrass. Gray leaf spot is a serious disease on St. Augustinegrass (Stenotaphrum secundatum), perennial ryegrass (Lolium perenne), and tall fescue (Festuca arundinacea). Virulence assays performed in this study revealed that M. grisea collected from rice could also cause disease on St. Augustinegrass and tall fescue. One rice isolate, Che86061, caused similar disease reactions on susceptible cultivars of rice and St. Augustinegrass and an incompatible interaction on resistant cultivars of both species. To explore whether …


Cell Wall Proteome In The Maize Primary Root Elongation Zone. Ii. Region-Specific Changes In Water Soluble And Lightly Ionically Bound Proteins Under Water Deficit1[W][Oa], Jinming Zhu, Sophie Alvarez, Ellen L. Marsh, Mary E. Lenoble, In-Jeong Cho, Mayandi Sivaguru, Sixue Chen, Henry T. Nguyen, Yajun Wu, Daniel P. Schachtman, Robert E. Sharp Jan 2007

Cell Wall Proteome In The Maize Primary Root Elongation Zone. Ii. Region-Specific Changes In Water Soluble And Lightly Ionically Bound Proteins Under Water Deficit1[W][Oa], Jinming Zhu, Sophie Alvarez, Ellen L. Marsh, Mary E. Lenoble, In-Jeong Cho, Mayandi Sivaguru, Sixue Chen, Henry T. Nguyen, Yajun Wu, Daniel P. Schachtman, Robert E. Sharp

Department of Agronomy and Horticulture: Faculty Publications

Previous work on the adaptation of maize (Zea mays) primary roots to water deficit showed that cell elongation is maintained preferentially toward the apex, and that this response involves modification of cell wall extension properties. To gain a comprehensive understanding of how cell wall protein (CWP) composition changes in association with the differential growth responses to water deficit in different regions of the elongation zone, a proteomics approach was used to examine water soluble and loosely ionically bound CWPs. The results revealed major and predominantly region-specific changes in protein profiles between well-watered and water-stressed roots. In total, 152 …


Map Location Of The Rpp1 Locus That Confers Resistance To Soybean Rust In Soybean, D. L. Hyten, G. L. Hartman, R. L. Nelson, R. D. Frederick, V. C. Concibido, J. M. Narvel, P. B. Cregan Jan 2007

Map Location Of The Rpp1 Locus That Confers Resistance To Soybean Rust In Soybean, D. L. Hyten, G. L. Hartman, R. L. Nelson, R. D. Frederick, V. C. Concibido, J. M. Narvel, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Soybean rust (SBR), caused by Phakopsora pachyrhizi, was first discovered in North America in 2004 and has the potential to become a major soybean [Glycine max (L.) Merr.] disease in the USA. Currently, four SBR resistance genes have been identifi ed but not mapped on the soybean genetic linkage map. One of these resistance genes is the Rpp1 gene, which is present in the soybean accession PI 200492. The availability of molecular markers associated with Rpp1 will permit marker-assisted selection and expedite the incorporation of this gene into U.S. cultivars. We compared simple sequence repeat (SSR) markers between …


Barcsoysnp23: A Panel Of 23 Selected Snps For Soybean Cultivar Identification, M. S. Yoon, Q.J. Song, I. Y. Choi, James E. Specht, D. L. Hyten, P. B. Cregan Jan 2007

Barcsoysnp23: A Panel Of 23 Selected Snps For Soybean Cultivar Identification, M. S. Yoon, Q.J. Song, I. Y. Choi, James E. Specht, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

This report describes a set of 23 informative SNPs (BARCSoySNP23) distributed on 19 of the 20 soybean linkage groups that can be used for soybean cultivar identification. Selection of the SNPs to include in this set was made based upon the information provided by each SNP for distinguishing a diverse set of soybean genotypes as well as the linkage map position of each SNP. The genotypes included the ancestors of North American cultivars, modern North American cultivars and a group of Korean cultivars. The procedure used to identify this subset of highly informative SNP markers resulted in a significant increase …


Ethylene Involvement In The Regulation Of The H+-Atpase Csha1 Gene And Of The New Isolated Ferric Reductase Csfro1 And Iron Transporter Csirt1 Genes In Cucumber Plants, Brian M. Waters, Carlos Lucena, Francisco J. Romera, Gena G. Jester, April N. Wynn, Carmen L. Rojas, Esteban Alcántara, Rafael Pérez-Vicente Jan 2007

Ethylene Involvement In The Regulation Of The H+-Atpase Csha1 Gene And Of The New Isolated Ferric Reductase Csfro1 And Iron Transporter Csirt1 Genes In Cucumber Plants, Brian M. Waters, Carlos Lucena, Francisco J. Romera, Gena G. Jester, April N. Wynn, Carmen L. Rojas, Esteban Alcántara, Rafael Pérez-Vicente

Department of Agronomy and Horticulture: Faculty Publications

In previous works using ethylene inhibitors and precursors, it has been shown that ethylene participates in the regulation of several Fe-deficiency stress responses by Strategy I plants, such as enhanced ferric reductase activity, rhizosphere acidification, and subapical root hair development. Furthermore, recent evidence suggests that ethylene could regulate the expression of both the ferric reductase and the iron transporter genes of Strategy I plants by affecting the FER (or FER-like) transcription factor. Recently, two H+-ATPase genes have been isolated from cucumber roots, CsHA1 and CsHA2. CsHA1 is up-regulated under Fe deficiency while CsHA2 is constitutively expressed. In …


Fertility Restoration Of The Sorghum A3 Male-Sterile Cytoplasm Through A Sporophytic Mechanism Derived From Sudangrass, Hoang V. Tang, Jeffrey F. Pedersen, Christine D. Chase, Daryl R. Pring Jan 2007

Fertility Restoration Of The Sorghum A3 Male-Sterile Cytoplasm Through A Sporophytic Mechanism Derived From Sudangrass, Hoang V. Tang, Jeffrey F. Pedersen, Christine D. Chase, Daryl R. Pring

Department of Agronomy and Horticulture: Faculty Publications

Fertility restoration of sorghum [Sorghum bicolor (L.) Moench] lines carrying the IS1112C (A3 group) male-sterile cytoplasm has been documented as a two-gene gametophytic mechanism involving complementary action of restoring alleles designated Rf3 and Rf4, as derived from IS1112C. Fertility restoration capability has also been reported from sudangrass (S. bicolor subsp. drummondii) populations. We describe characteristics of a fertility restoration system derived from sudangrass, in which male-sterile individuals were observed at high frequency in backcross and F2-F3 segregating populations. Segregation analyses were consistent with a sporophytic restoration system involving two complementary genes. Pollen iodine staining in fertile …


Array-Based Genotyping And Expression Analysis Of Barley Cv. Maythorpe And Golden Promise, Harkamal Walia, Clyde Wilson, Pascal Condamine, Abdelbagi M. Ismail, Jin Xu, Xinping Cui, Timothy J. Close Jan 2007

Array-Based Genotyping And Expression Analysis Of Barley Cv. Maythorpe And Golden Promise, Harkamal Walia, Clyde Wilson, Pascal Condamine, Abdelbagi M. Ismail, Jin Xu, Xinping Cui, Timothy J. Close

Department of Agronomy and Horticulture: Faculty Publications

Background: Golden Promise is a salt-tolerant spring barley closely related to Maythorpe. Salt tolerance in Golden Promise has been attributed to a single mutation at the Ari-e locus (on 5H) resulting from irradiation of Maythorpe. Golden Promise accumulates lower shoot Na+ compared to Maythorpe when growing under saline conditions. This study focused on elucidating the genetic basis and mechanisms involved in this difference. Results: The level of polymorphism between the two genotypes was explored using the Barley1 GeneChip for single feature polymorphisms (SFPs) and an oligonucleotide pool assay for single nucleotide polymorphisms (SNPs). Polymorphism analyses revealed three haplotype blocks …


Nutrient Use Efficiency: Measurement And Management, Achim Dobermann Jan 2007

Nutrient Use Efficiency: Measurement And Management, Achim Dobermann

Department of Agronomy and Horticulture: Faculty Publications

Mineral fertilizers have sustained world agriculture and thus global population and wealth growth for more than 100 years (Smil, 2001; Stewart et al., 2005). Their contribution to increasing crop yields has spared millions of hectares of natural ecosystems that otherwise would have been converted to agriculture (Balmford et al., 2005). However, lacking, imbalanced, inappropriate or excessive use of nutrients in agricultural systems remains a concern. Nutrient mining is a major cause for low crop yields in parts of the developing world, particularly Africa. In other situations, nutrients such as nitrogen (N) and phosphorus (P) often move beyond the bounds of …


Whole-Genome Expression Profiling Defines The Hrpl Regulon Of Pseudomonas Syringae Pv. Tomato Dc3000, Allows De Novo Reconstruction Of The Hrp Cis Element, And Identifies Novel Coregulated Genes, Adriana O. Ferreira, Christopher R. Myers, Jeffrey S. Gordon, Gregory B. Martin, Monica Vencato, Alan Collmer, Misty D. Wehling, James R. Alfano, Gabriel Moreno-Hagelsieb, Warren F. Lamboy, Geneviene Declerck, David J. Schneider, Samuel W. Cartinhour Nov 2006

Whole-Genome Expression Profiling Defines The Hrpl Regulon Of Pseudomonas Syringae Pv. Tomato Dc3000, Allows De Novo Reconstruction Of The Hrp Cis Element, And Identifies Novel Coregulated Genes, Adriana O. Ferreira, Christopher R. Myers, Jeffrey S. Gordon, Gregory B. Martin, Monica Vencato, Alan Collmer, Misty D. Wehling, James R. Alfano, Gabriel Moreno-Hagelsieb, Warren F. Lamboy, Geneviene Declerck, David J. Schneider, Samuel W. Cartinhour

Department of Plant Pathology: Faculty Publications

Pseudomonas syringae pv. tomato DC3000 is a model pathogen of tomato and Arabidopsis that uses a hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS) to deliver virulence effector proteins into host cells. Expression of the Hrp system and many effector genes is activated by the HrpL alternative sigma factor. Here, an open reading frame-specific whole-genome microarray was constructed for DC3000 and used to comprehensively identify genes that are differentially expressed in wild-type and ΔhrpL strains. Among the genes whose differential regulation was statistically significant, 119 were upregulated and 76 were downregulated in the wild-type compared with the ΔhrpL …


Microbial Content Of Abattoir Wastewater And Its Contaminated Soil In Lagos, Nigeria, A. O. Adesemoye, B. O. Opere, S. C. O. Makinde Oct 2006

Microbial Content Of Abattoir Wastewater And Its Contaminated Soil In Lagos, Nigeria, A. O. Adesemoye, B. O. Opere, S. C. O. Makinde

Department of Plant Pathology: Faculty Publications

Microbial content of wastewater in two abattoirs and the impact on microbial population of receiving soil was studied in Agege and Ojo Local Government Areas in Lagos State, Nigeria. Wastewater samples were collected from each of the abattoirs over three months period and examined for microbial content. Soil samples contaminated with the wastewaters were also collected and analyzed for microbial content as compared to soil without wastewater contamination in the neighborhood (control). Some physico-chemical parameters of the samples such as total dissolved solid, chemical oxygen demand, etc., were examined. The wastewater samples from both abattoirs were highly contaminated; Agege abattoir …


Inducible Yeast System For Viral Rna Recombination Reveals Requirement For An Rna Replication Signal On Both Parental Rnas, Hernan Garcia-Ruiz, Paul Ahlquist Sep 2006

Inducible Yeast System For Viral Rna Recombination Reveals Requirement For An Rna Replication Signal On Both Parental Rnas, Hernan Garcia-Ruiz, Paul Ahlquist

Department of Plant Pathology: Faculty Publications

To facilitate RNA recombination studies, we tested whether Saccharomyces cerevisiae, which supports brome mosaic virus (BMV) replication, also supports BMV RNA recombination. Yeast strains expressing BMV RNA replication proteins 1a and 2apol were engineered to transiently coexpress two independently inducible, overlapping, nonreplicating derivatives of BMV genomic RNA3. B33' lacked the coat protein gene and negative-strand RNA promoter. B35' lacked the positive-strand RNA promoter and had the coat gene replaced by the selectable URA3 gene. After 12 to 72 h of induction, B33' and B35' transcription was repressed and Ura+ yeast cells were selected. All Ura+ cells …


Bacillus Species Nrrl B-30212 For Reducing Fusarium Head Blight In Cereals, David A. Schisler, Naseem L. Khan, Michael J. Boehm Feb 2006

Bacillus Species Nrrl B-30212 For Reducing Fusarium Head Blight In Cereals, David A. Schisler, Naseem L. Khan, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Four yeasts (NRRL Y-30213, NRRL Y-30214, NRRL Y-30215, and NRRL Y-30216) and 1 bacterium (NRRL B-30212) have been identified as being superior antagonists capable of suppressing Fusarium head blight (head scab) in cereals, particularly in wheat and barley. Fusarium head blight is primarily caused by the fungus Gibberella zeae (anamorph=Fusarium graminearum).


Carbon-To-Nitrogen Ratio And Carbon Loading Of Production Media Influence Freeze-Drying Survival And Biocontrol Efficacy Of Cryptococcus Nodaensis Oh 182.9, Shouan Zhang, David A. Schisler, Michael J. Boehm, Patricia J. Slininger Jan 2006

Carbon-To-Nitrogen Ratio And Carbon Loading Of Production Media Influence Freeze-Drying Survival And Biocontrol Efficacy Of Cryptococcus Nodaensis Oh 182.9, Shouan Zhang, David A. Schisler, Michael J. Boehm, Patricia J. Slininger

Department of Plant Pathology: Faculty Publications

Fusarium head blight (FHB), caused by Gibberella zeae, is a devastating disease of wheat worldwide. Cryptococcus nodaensis OH 182.9 is an effective biocontrol agent for this disease. Development of a dried product of OH 182.9 would have potential advantages of ease of handling, favorable economics, and acceptance by end users. Isolate OH 182.9 was grown for 48 and 72 h in semi-defined complete liquid (SDCL) medium with carbon-to-nitrogen (C/N) ratios of 6.5:1, 9:1, 11:1, 15:1, and 30:1, and in SDCL C/N 30:1 media with varied carbon loadings of 7, 14, 21, and 28 g/liter. Total biomass production and cell …


Influence Of Nutrient Availability, Stand Age, And Canopy Structure On Isoprene Flux In A Eucalyptus Saligna Experimental Forest, Jennifer L. Funk, Christian P. Giardina, Alexander Knohl, Manuel T. Lerdau Jan 2006

Influence Of Nutrient Availability, Stand Age, And Canopy Structure On Isoprene Flux In A Eucalyptus Saligna Experimental Forest, Jennifer L. Funk, Christian P. Giardina, Alexander Knohl, Manuel T. Lerdau

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Eucalyptus plantations occupy approximately 10 million ha of land in the tropics and, increasingly, afforestation and reforestation projects are relying on this genus to provide rapid occupation of degraded sites, large quantities of high-quality wood products, and high rates of carbon sequestration. Members of the genus Eucalyptus are also very high emitters of isoprene, the dominant volatile organic compound emitted by trees in tropical ecosystems, which significantly influences the oxidative capacity of the atmosphere. While fertilization growth response of these trees has been intensively studied, little is known about how fertilization and tree age alter isoprene production from plantations of …


Snp-Phage – High Throughput Snp Discovery Pipeline, Lakshmi K. Matukumalli, John J. Grefenstette, David L. Hyten, Ik-Young Choi, Perry B. Cregan, Curt P. Van Tassell Jan 2006

Snp-Phage – High Throughput Snp Discovery Pipeline, Lakshmi K. Matukumalli, John J. Grefenstette, David L. Hyten, Ik-Young Choi, Perry B. Cregan, Curt P. Van Tassell

Department of Agronomy and Horticulture: Faculty Publications

Background: Single nucleotide polymorphisms (SNPs) as defined here are single base sequence changes or short insertion/deletions between or within individuals of a given species. As a result of their abundance and the availability of high throughput analysis technologies SNP markers have begun to replace other traditional markers such as restriction fragment length polymorphisms (RFLPs), amplified fragment length polymorphisms (AFLPs) and simple sequence repeats (SSRs or microsatellite) markers for fine mapping and association studies in several species. For SNP discovery from chromatogram data, several bioinformatics programs have to be combined to generate an analysis pipeline. Results have to be stored in …


First Report Of Sudden Death Syndrome Of Soybean Caused By Fusarium Solani F. Sp. Glycines In Nebraska, A. D. Ziems, L. J. Giesler, G. Y. Yuen Jan 2006

First Report Of Sudden Death Syndrome Of Soybean Caused By Fusarium Solani F. Sp. Glycines In Nebraska, A. D. Ziems, L. J. Giesler, G. Y. Yuen

Department of Plant Pathology: Faculty Publications

During August of 2004, soybean (Glycine max (L.) Merr.) plants exhibiting symptoms typical of sudden death syndrome (SDS) caused by Fusarium solani (Mart.) Sacc. f. sp. glycines (= Fusarium virguliforme Akoi, O'Donnell, Homma, & Lattanzi) (1) were observed in Nemaha and Pierce counties in eastern Nebraska. Leaf symptoms ranged from small chlorotic spots to prominent interveinal necrosis on plants at R5-R6 growth stages. Taproots of symptomatic plants were plated on potato dextrose agar (PDA) amended with hymexazol, ampicillin, and rifampicin (HAR). Resulting fungal isolates grew slowly and developed masses of blue macroconidia, characteristic of F. solani f. sp. glycines …


Avenacin Production In Creeping Bentgrass (Agrostis Stolonifera) And Its Influence On The Host Range Of Gaeumannomyces Graminis, S. L. Thomas, P. Bonello, P. E. Lipps, Michael J. Boehm Jan 2006

Avenacin Production In Creeping Bentgrass (Agrostis Stolonifera) And Its Influence On The Host Range Of Gaeumannomyces Graminis, S. L. Thomas, P. Bonello, P. E. Lipps, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Avenacinase activity has been shown to be a key factor determining the host range of Gaeumannomyces graminis on oats (Avena sativa). G. graminis var. avenae produces avenacinase, which detoxifies the oat root saponin avenacin, enabling it to infect oats. G. graminis var. tritici does not produce avenacinase and is unable to infect oats. G. graminis var. avenae is also reported to incite take-all patch on creeping bentgrass (Agrostis stolonifera). It is unknown whether creeping bentgrass produces avenacin and if the avenacin-avenacinase interaction influences G. graminis pathogenicity on creeping bentgrass. The root extracts of six creeping bentgrass …


Fungicide Sensitivity Of Sclerotinia Homoeocarpa From Golf Courses In Ohio, Young-Ki Jo, Amy L. Niver, Joseph W. Rimelspach, Michael J. Boehm Jan 2006

Fungicide Sensitivity Of Sclerotinia Homoeocarpa From Golf Courses In Ohio, Young-Ki Jo, Amy L. Niver, Joseph W. Rimelspach, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Managing dollar spot, the most common and chronic disease on intensively cultivated turfgrass, relies on the judicious use of fungicides. The heavy use of fungicides has led to the development of isolates of Sclerotinia homoeocarpa insensitive to several classes of fungicides, including benzimidazoles, demethylation-inhibitors, and dicarboximides. In vitro fungicide sensitivity assays using single discriminatory concentrations of thiophanate-methyl, propiconazole, and iprodione were developed in this study for evaluating field efficacy of these fungicides and the prevalence of fungicide insensitivity within S. homoeocarpa isolated from golf courses throughout Ohio. Discriminatory concentrations for these fungicides were determined to be: thiophanate-methyl = 1,000 μg …


Evaluation Of The Single Kernel Characterization System (Skcs) For Measurement Of Sorghum Grain Attributes, S. R. Bean, O. K. Chung, M. R. Tuinstra, Jeffrey F. Pedersen, J. Erpelding Jan 2006

Evaluation Of The Single Kernel Characterization System (Skcs) For Measurement Of Sorghum Grain Attributes, S. R. Bean, O. K. Chung, M. R. Tuinstra, Jeffrey F. Pedersen, J. Erpelding

Department of Agronomy and Horticulture: Faculty Publications

The single kernel characterization system (SKCS) has been widely used in the wheat industry, and SKCS parameters have been linked to end-use quality in wheat. The SKCS has promise as a tool for evaluating sorghum grain quality. However, the SKCS was designed to analyze wheat, which has a different kernel structure from sorghum. To gain a better understanding of the meaning of SKCS predictions for grain sorghum, individual sorghum grains were measured for length, width, thickness (diameter), and weight by laboratory methods and by the SKCS. SKCS predictions for kernel weight and thickness were highly correlated to laboratory measurements. However, …


Application Of Machine Learning In Snp Discovery, Lakshmi K. Matukumalli, John J. Grefenstette, David L. Hyten, Ik-Young Choi, Perry B. Cregan, Curtis P. Van Tassell Jan 2006

Application Of Machine Learning In Snp Discovery, Lakshmi K. Matukumalli, John J. Grefenstette, David L. Hyten, Ik-Young Choi, Perry B. Cregan, Curtis P. Van Tassell

Department of Agronomy and Horticulture: Faculty Publications

Background: Single nucleotide polymorphisms (SNP) constitute more than 90% of the genetic variation, and hence can account for most trait differences among individuals in a given species. Polymorphism detection software PolyBayes and PolyPhred give high false positive SNP predictions even with stringent parameter values. We developed a machine learning (ML) method to augment PolyBayes to improve its prediction accuracy. ML methods have also been successfully applied to other bioinformatics problems in predicting genes, promoters, transcription factor binding sites and protein structures. Results: The ML program C4.5 was applied to a set of features in order to build a SNP classifier …


Chemical Composition And Response To Dilute-Acid Pretreatment And Enzymatic Saccharification Of Alfalfa, Reed Canarygrass, And Switchgrass, Bruce S. Dien, Hans-Joachim G. Jung, Kenneth P. Vogel, Michael D. Casler, Joann F. S. Lamb, Loren Iten, Robert C. Mitchell, Gautum Sarath Jan 2006

Chemical Composition And Response To Dilute-Acid Pretreatment And Enzymatic Saccharification Of Alfalfa, Reed Canarygrass, And Switchgrass, Bruce S. Dien, Hans-Joachim G. Jung, Kenneth P. Vogel, Michael D. Casler, Joann F. S. Lamb, Loren Iten, Robert C. Mitchell, Gautum Sarath

Department of Agronomy and Horticulture: Faculty Publications

Alfalfa stems, reed canarygrass, and switchgrass; perennial herbaceous species that have potential as biomass energy crops in temperate regions; were evaluated for their bioconversion potential as energy crops. Each forage species was harvested at two or three maturity stages and analyzed for carbohydrates, lignin, protein, lipid, organic acids, and mineral composition. The biomass samples were also evaluated for sugar yields following pretreatment with dilute sulfuric followed by enzymatic saccharification using a commercial cellulase preparation. Total carbohydrate content of the plants varied from 518 to 655 g kg-1 dry matter (DM) and cellulose concentration from 209 to 322 g kg …


Mutations In Arabidopsis Yellow Stripe-Like1 And Yellow Stripe-Like3 Reveal Their Roles In Metal Ion Homeostasis And Loading Of Metal Ions In Seeds, Brian M. Waters, Heng-Hsuan Chu, Raymond J. Didonato, Louis A. Roberts, Robynn B. Eisley, Brett Lahner, David E. Salt, Elsbeth L. Walker Jan 2006

Mutations In Arabidopsis Yellow Stripe-Like1 And Yellow Stripe-Like3 Reveal Their Roles In Metal Ion Homeostasis And Loading Of Metal Ions In Seeds, Brian M. Waters, Heng-Hsuan Chu, Raymond J. Didonato, Louis A. Roberts, Robynn B. Eisley, Brett Lahner, David E. Salt, Elsbeth L. Walker

Department of Agronomy and Horticulture: Faculty Publications

Here, we describe two members of the Arabidopsis (Arabidopsis thaliana) Yellow Stripe-Like (YSL) family, AtYSL1 and AtYSL3. The YSL1 and YSL3 proteins are members of the oligopeptide transporter family and are predicted to be integral membrane proteins. YSL1 and YSL3 are similar to the maize (Zea mays) YS1 phytosiderophore transporter (ZmYS1) and the AtYSL2 iron (Fe)-nicotianamine transporter, and are predicted to transport metal-nicotianamine complexes into cells. YSL1 and YSL3 mRNAs are expressed in both root and shoot tissues, and both are regulated in response to the Fe status of the plant. β-Glucuronidase reporter expression, driven by …


First Report Of Leaf Spot Of Smooth Bromegrass Caused By Pithomyces Chartarum In Nebraska, C. Eken, C. C. Jochum, G. Y. Yuen Jan 2006

First Report Of Leaf Spot Of Smooth Bromegrass Caused By Pithomyces Chartarum In Nebraska, C. Eken, C. C. Jochum, G. Y. Yuen

Department of Plant Pathology: Faculty Publications

Smooth bromegrass (Bromus inermis Leyss.) is the most common perennial grass species cultivated for forage in North America. During late fall of 2004, smooth bromegrass plants in Lincoln, NE were observed to have brown lesions on leaf midveins that were several centimeters long. Symptomatic leaves were surface disinfested for 1 min in 2% NaOCl and incubated at 25°C on potato dextrose agar (PDA) and water agar. The fungus, Pithomyces chartarum (Berk. & Curt) Ellis, was isolated consistently and identified on the basis of morphological characteristics (1). Colonies were effused and black on PDA. Conidiophores measured 3.5 to 8 …


Bioinformatics-Enabled Identification Of The Hrpl Regulon And Type Iii Secretion System Effector Proteins Of Pseudomonas Syringae Pv. Phaseolicola 1448a, Monica Vencato, Fang Tian, James R. Alfano, C. Robin Buell, Samuel Cartinhour, Genevieve A. Declerck, David S. Guttman, John Stavrinides, Vinita Joardar, Magdalen Lindeberg, Philip A. Bronstein, John W. Mansfield, Christopher R. Myers, Alan Collmer, David J. Schneider Jan 2006

Bioinformatics-Enabled Identification Of The Hrpl Regulon And Type Iii Secretion System Effector Proteins Of Pseudomonas Syringae Pv. Phaseolicola 1448a, Monica Vencato, Fang Tian, James R. Alfano, C. Robin Buell, Samuel Cartinhour, Genevieve A. Declerck, David S. Guttman, John Stavrinides, Vinita Joardar, Magdalen Lindeberg, Philip A. Bronstein, John W. Mansfield, Christopher R. Myers, Alan Collmer, David J. Schneider

Department of Plant Pathology: Faculty Publications

The ability of Pseudomonas syringae pv. phaseolicola to cause halo blight of bean is dependent on its ability to translocate effector proteins into host cells via the hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS). To identify genes encoding type III effectors and other potential virulence factors that are regulated by the HrpL alternative sigma factor, we used a hidden Markov model, weight matrix model, and type III targeting-associated patterns to search the genome of P. syringae pv. phaseolicola 1448A, which recently was sequenced to completion. We identified 44 high-probability putative Hrp promoters upstream of genes encoding the …


Regulation Of Hyphal Morphogenesis And The Dna Damage Response By The Aspergillus Nidulans Atm Homolog Atma, Iran Malavazi, Camile P. Semighini, Marcia Regina Von Zeska Kress, Steven D. Harris, Gustavo H. Goldman Jan 2006

Regulation Of Hyphal Morphogenesis And The Dna Damage Response By The Aspergillus Nidulans Atm Homolog Atma, Iran Malavazi, Camile P. Semighini, Marcia Regina Von Zeska Kress, Steven D. Harris, Gustavo H. Goldman

Department of Plant Pathology: Faculty Publications

Ataxia telangiectasia (A-T) is an inherited disorder characterized by progressive loss of motor function and susceptibility to cancer. The most prominent clinical feature observed in A-T patients is the degeneration of Purkinje motor neurons. Numerous studies have emphasized the role of the affected gene product, ATM, in the regulation of the DNA damage response. However, in Purkinje cells, the bulk of ATM localizes to the cytoplasm and may play a role in vesicle trafficking. The nature of this function, and its involvement in the pathology underlying A-T, remain unknown. Here we characterize the homolog of ATM (AtmA) in the filamentous …


Soybean Cultivars Resulted From More Recombination Events Than Unselected Lines In The Same Population, T. R. Stefaniak, D. L. Hyten Jr., V. R. Pantalone, A. Klarer, T. W. Pfeiffer Jan 2006

Soybean Cultivars Resulted From More Recombination Events Than Unselected Lines In The Same Population, T. R. Stefaniak, D. L. Hyten Jr., V. R. Pantalone, A. Klarer, T. W. Pfeiffer

Department of Agronomy and Horticulture: Faculty Publications

The selection of superior adapted cultivars has contributed to the doubling of soybean [Glycine max (L.) Merr.] yields in the USA since 1930. Genetic variation was required for this selection to be effective. The objective of this study was to evaluate the importance of homologous chromosome meiotic recombination in the creation of soybean cultivars. A set of 10 adapted high-yielding cultivars selected from the cross ‘Williams’ × ‘Essex’ was com-pared with a set of 156 random recombinant inbred lines (RILs) from the same population. Crossover events were identified using 143 simple sequence repeat (SSR) markers span-ning all 20 soybean …