Other Plant Sciences Commons^™

Overexpression Of The Sorghum Bicolor Sbccoaomt Alters Cell Wall Associated Hydroxycinnamoyl Groups, Hannah M. Tetreault, Erin D. Scully, Tammy Gries, Nathan A. Palmer, Deanna L. Funnell-Harris, Bruce S. Dien, Gautam Sarath, Thomas E. Clemente, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

Sorghum (Sorghum bicolor) is a drought tolerant crop, which is being developed as a bioenergy feedstock. The monolignol biosynthesis pathway is a major focus for altering the abundance and composition of lignin. Caffeoyl coenzyme-A O-methyltransferase (CCoAOMT) is an S-adenosyl methionine (SAM)-dependent O-methyltransferase that methylates caffeoyl-CoA to generate feruloyl-CoA, an intermediate required for the biosynthesis of both G- and S-lignin. SbCCoAOMT was overexpressed to assess the impact of increasing the amount of this enzyme on biomass composition. SbCCoAOMT overexpression increased both soluble and cell wall-bound (esterified) ferulic and sinapic acids, however lignin concentration and its composition …

Elucidating Sorghum Biomass, Nitrogen And Chlorophyll Contents With Spectral And Morphological Traits Derived From Unmanned Aircraft System, Jiating Li, Yeyin Shi, Arun-Narenthiran Veeranampalayam-Sivakumar, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Unmanned aircraft systems (UAS) provide an efficient way to phenotype cropmorphology with spectral traits such as plant height, canopy cover and various vegetation indices (VIs) providing information to elucidate genotypic responses to the environment. In this study, we investigated the potential use of UAS-derived traits to elucidate biomass, nitrogen and chlorophyll content in sorghum under nitrogen stress treatments. A nitrogen stress trial located in Nebraska, USA, contained 24 different sorghum lines, 2 nitrogen treatments and 8 replications, for a total of 384 plots. Morphological and spectral traits including plant height, canopy cover and various VIs were derived from UAS flights …

Nebline, October 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Nebraska Extension’s CHIME program: Enhancing childcare professionals’ well-being with mindfulness

Food & Health

Farm & Acreage

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Horticulture

Early Childhood

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The Effect Of Ingredient-Specific Calorie Information On Calories Ordered, Christopher R. Gustafson, Eliana Zeballos

Department of Agronomy and Horticulture: Faculty Publications

Providing calorie labeling is a widely used strategy to combat obesity. However, there is little evidence that the availability of calorie information at food-away-from-home establishments has an effect on food choices. Listing calorie information for each ingredient, though, may allow customers to avoid high-calorie items that add little to their enjoyment. Data from a natural experiment were used to compare total calories ordered before and after the provision of per-ingredient versus for build-your-own sandwiches, and per-sandwich for pre-defined sandwiches, at a supermarket sandwich counter. Sandwich order slips from a Lincoln, Neb. supermarket were collected from December 15, 2016 to February …

Phylogenomic Evidence For Ancient Recombination Between Plastid Genomes Of The Cupressus-Juniperus-Xanthocyparis Complex (Cupressaceae), Andan Zhu, Weishu Fan, Robert P. Adams, Jeffrey P. Mower

Department of Agronomy and Horticulture: Faculty Publications

Background: Phylogenetic relationships among Eastern Hemisphere cypresses, Western Hemisphere cypresses, junipers, and their closest relatives are controversial, and generic delimitations have been in flux for the past decade. To address relationships and attempt to produce a more robust classification, we sequenced 11 new plastid genomes (plastomes) from the five variously described genera in this complex (Callitropsis, Cupressus, Hesperocyparis, Juniperus, and Xanthocyparis) and compared them with additional plastomes from diverse members of Cupressaceae.

Results: Phylogenetic analysis of protein-coding genes recovered a topology in which Juniperus is sister to Cupressus, whereas a tree based on …

Nebline, September 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Septic systems need regular maintenance to function properly

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Mobile Elements Shape Plastome Evolution In Ferns, Tanner A. Robison, Amanda L. Grusz, Paul G. Wolf, Jeffrey P. Mower, Blake D. Fauskee, Karla Sosa, Eric Schuettpelz

Department of Agronomy and Horticulture: Faculty Publications

Plastid genomes display remarkable organizational stability over evolutionary time. From green algae to angiosperms, most plastid genomes are largely collinear, with only a few cases of inversion, gene loss, or, in extremely rare cases, gene addition. These plastome insertions are mostly clade-specific and are typically of nuclear or mitochondrial origin. Here, we expand on these findings and present the first family-level survey of plastome evolution in ferns, revealing a novel suite of dynamic mobile elements. Comparative plastome analyses of the Preridaceae expose several mobile open reading frames that vary in sequence length, insertion site, and configuration among sampled taxa. Even …

The Evaluation Of Two Different Harvesting 'Topbunch' And 'Hi-Crop' Collards (Brassica Oleracea (L)) Leaves From Plants Grown In A Wiregrass Tunnel House, Edward Sparks, Raymon Shange, Jacquelyn Jackson, Victor Khan, James E. Currington, Ramble Ankumah, Nathaniel Ellison, George X. Hunter Jr., Jeffery L. Moore

Professional Agricultural Workers Journal

A study was conducted to determine if a 100% or 50% harvest intensity of ‘Topbunch’ collards leaves could be a recommended practice for Tunnel House producers. The experiment was conducted as a split-plot design with harvest dates as main plots, and harvesting intensity of 100% or 50% of leaves as sub-plots. All treatments were replicated three times, drip irrigated, and fertilized according to soil test recommendations. The results showed significant interactions between harvest methods and dates for number and weight of leaves harvested. There were also significant differences for the weight of leaves harvested and numbers. The leaf recovery rates …

Nebline, August 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Nutrition Education Kits developed by Extension help LPS students learn about nutrition and physical activity

Food & Health

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Special pullout section: Lancaster County Super Fair 2018 Schedule & Map

Evaluation Of Glyphosate And Ppo-Inhibiting Herbicide Tank-Mixtures To Manage Glyphosate Resistance In Soybean, Jesaelen Gizotti De Moraes

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Protoporphyrinogen oxidase (PPO)-inhibiting herbicides in combination with glyphosate for postemergence (POST) applications is one of the primary alternatives to manage glyphosate-resistant weeds and the only effective POST chemical option in conventional and glyphosate-tolerant soybean to control glyphosate and ALS-inhibiting resistant weeds. Antagonistic interactions have been reported between many different herbicide modes of action and optimal droplet size may be affected by tank-mixtures of different herbicides. Additionally, the impact of adjuvants on the factors aforementioned as well as on physical properties needs to be thoroughly investigate to maximize herbicide efficacy. Therefore, the objectives of this research were to: 1) conduct greenhouse …

Utilizing Random Regression Models For Genomic Prediction Of A Longitudinal Trait Derived From High‐Throughput Phenotyping, Malachy T. Campbell, Harkamal Walia, Gota Morota

Department of Agronomy and Horticulture: Faculty Publications

The accessibility of high‐throughput phenotyping platforms in both the greenhouse and field, as well as the relatively low cost of unmanned aerial vehicles, has provided researchers with an effective means to characterize large populations throughout the growing season. These longitudinal phenotypes can provide important insight into plant development and responses to the environment. Despite the growing use of these new phenotyping approaches in plant breeding, the use of genomic prediction models for longitudinal phenotypes is limited in major crop species. The objective of this study was to demonstrate the utility of random regression (RR) models using Legendre polynomials for genomic …

Identification Of Genomic Regions Contributing To Protein Accumulation In Wheat Under Well-Watered And Water Deficit Growth Conditions, Ibrahim S. Elbasyoni, Sabah M. Morsy, Raghuprakash Kastoori Ramamurthy, Atef M. Nassar

Department of Agronomy and Horticulture: Faculty Publications

Sustaining wheat production under low-input conditions through development and identifying genotypes with enhanced nutritional quality are two current concerns of wheat breeders. Wheat grain total protein content, to no small extent, determines the economic and nutritive value of wheat. Therefore, the objectives of this study are to identify accessions with high and low grain protein content (GPC) under well-watered and water-deficit growth conditions and to locate genomic regions that contribute to GPC accumulation. Spring wheat grains obtained from 2111 accessions that were grown under well-watered and water-deficit conditions were assessed for GPC using near-infrared spectroscopy (NIR). Results indicated significant influences …

Nebline, July 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Pretty things that sting

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Nebline, June 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Nebraska Pollinator Habitat Certification program

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Soil Science In Precision Agriculture Lesson Plan, Carson Ellis Cronkright

Agricultural Education and Communication

Due to the increasing demands for the American agricultural industry to provide exceptional products at an increasing rate of production, as well as implement the practice of sustainable farming, it is imperative that more modern concepts be taught at a younger age (Ray, 2013). In order to inform students of advancements and the future of agriculture in the United States, curriculum and lesson plans should reflect this movement in industry in order to provide relevant information and maintain the highest quality of education possible. This project will provide a constructed lesson plan as one example of adopting more progressive methods …

Nebline, May 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Farmers: Use climate for planning, weather for doing

Farm & Acreage

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Nebline, April 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Can You Pass This Food Safety Quiz?

Food & Health

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Special Pullout Section: Weed Awareness

Impacts Of Cover Crop And Residue Removal On Soil Hydraulic And Thermal Properties, Michael T. Sindelar

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Large-scale crop residue removal for livestock or biofuel production may negatively affect soil and water resources. A combination of management practices could be the key to manage such resources under increasingly variable climate. For instance, use of cover crops (CCs) could offset the negative impacts that corn (Zea mays L.) residue removal may have on soil water and energy balance. We studied: 1) the effect of corn residue removal (56%) with and without winter rye (Secale cereale L.) CC on soil hydraulic and thermal properties including water infiltration, water retention, pores-size distribution, thermal conductivity, specific heat capacity, and …

Vegetation And Soil Responses To Concrete Grinding Residue Application On Highway Roadsides Of Eastern Nebraska, Ana Wingeyer, Martha Mamo, Walter H. Schacht, Dennis L. Mccallister, Pamela Sutton

Department of Agronomy and Horticulture: Faculty Publications

As a precautionary principle, the National Pollutant Discharge Elimination System (NPDES) permit establishes that the primary pollutant in concrete grinding residue (CGR) is its alkalinity and restricts CGR roadside discharge to 11 Mg ha⁻¹ or the agronomic liming rate, whichever is lower. We evaluated the effect of CGR application on roadside soil chemical properties, existing vegetation, and rainfall runoff. Five CGR rates (0, 11, 22, 45, and 90 dry Mg ha⁻¹) were tested on roadsides slopes at two different locations in eastern Nebraska. Vegetation, soil, and runoff characteristics were evaluated before CGR application and 30 d and …

A Systems Modeling Approach To Forecast Corn Economic Optimum Nitrogen Rate, Laila A. Puntel, John E. Sawyer, Daniel W. Barker, Peter J. Thorburn, Michael J. Castellano, Kenneth J. Moore, Andy Vanloocke, Emily A. Heaton, Sotirios V. Archontoulis

Department of Agronomy and Horticulture: Faculty Publications

Historically crop models have been used to evaluate crop yield responses to nitrogen (N) rates after harvest when it is too late for the farmers to make in-season adjustments. We hypothesize that the use of a crop model as an in-season forecast tool will improve current N decision-making. To explore this, we used the Agricultural Production Systems sIMulator (APSIM) calibrated with long-term experimental data for central Iowa, USA (16-years in continuous corn and 15-years in soybean-corn rotation) combined with actual weather data up to a specific crop stage and historical weather data thereafter. The objectives were to: (1) evaluate the …

Nebline, March 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Early Spring Care for Fruit Trees

Food & Health

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Review Of Genome-Wide Association Studies: From Polymorphism To Personalized Medicine, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

Genome-wide association studies (GWAS) has become a powerful tool in the area of quantitative genetics to map the relationship between trait and genomic variations. This volume provides a great resource for beginners to learn about the recent advances in GWAS and for domain experts to identify the gaps in the area. The first part of the volume lays out the statistical background of GWAS. I really liked the article by Yang et al., Introduction to Statistical Methods in Genome-Wide Association Studies. In this chapter, the authors talked about the missing heritability issue and introduced ways to calculate heritability using the …

An Epigenetic Breeding System In Soybean For Increased Yield And Stability, Sunil K. Kenchanmane Raju, Mon-Ray Shao, Robersy Sanchez, Ying-Zhi Xu, Ajay Sandhu, George L. Graef, Sally A. Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Epigenetic variation has been associated with a wide range of adaptive phenotypes in plants, but there exist few direct means for exploiting this variation. RNAi suppression of the plant-specific gene, MutS HOMOLOG1 (MSH1), in multiple plant species produces a range of developmental changes accompanied by modulation of defence, phytohormone and abiotic stress response pathways along with methylome repatterning. This msh1-conditioned developmental reprogramming is retained independent of transgene segregation, giving rise to transgene-null ‘memory’ effects. An isogenic memory line crossed to wild type produces progeny families displaying increased variation in adaptive traits that respond to selection. This study …

Inheritance Of Mesotrione Resistance In An Amaranthus Tuberculatus (Var. Rudis) Population From Nebraska, Usa, Maxwel C. Oliveira, Todd A. Gaines, Amit J. Jhala, Stevan Z. Knezevic

Department of Agronomy and Horticulture: Faculty Publications

A population of Amaranthus tuberculatus (var. rudis) evolved resistance to 4- hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicides (mesotrione, tembotrione, and topramezone) in Nebraska. The level of resistance was the highest to mesotrione, and the mechanism of resistance in this population is metabolism-based likely via cytochrome P450 enzymes. The increasing number of weeds resistant to herbicides warrants studies on the ecology and evolutionary factors contributing for resistance evolution, including inheritance of resistance traits. In this study, we investigated the genetic control of mesotrione resistance in an A. tuberculatus population from Nebraska, USA. Results showed that reciprocal crosses in the F1 families exhibited …

Nebline, February 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: The Expanded Food and Nutrition Education Program Teaches Limited-Resource Families Through Peer Education

Food & Health

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Pests & Wildlife

Horticulture

Early Childhood

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Genome-Wide Analysis Of Grain Yield Stability And Environmental Interactions In A Multiparental Soybean Population, Alencar Xavier, Diego Jarquin, Reka Howard, Vishnu Ramasubramanian, James E. Specht, George L. Graef, William D. Beavis, Brian W. Diers, Qijian Song, Perry B. Cregan, Randall L. Nelson, Rouf Mian, J. Grover Shannon, Leah K. Mchale, Dechun Wang, William Schapaugh, Aaron J. Lorenz, Shizhong Xu, William M. Muir, Katy M. Rainey

Department of Agronomy and Horticulture: Faculty Publications

Genetic improvement toward optimized and stable agronomic performance of soybean genotypes is desirable for food security. Understanding how genotypes perform in different environmental conditions helps breeders develop sustainable cultivars adapted to target regions. Complex traits of importance are known to be controlled by a large number of genomic regions with small effects whose magnitude and direction are modulated by environmental factors. Knowledge of the constraints and undesirable effects resulting from genotype by environmental interactions is a key objective in improving selection procedures in soybean breeding programs. In this study, the genetic basis of soybean grain yield responsiveness to environmental factors …

Overlapping Residual Herbicides For Control Of Photosystem (Ps) Ii- And 4-Hydroxyphenylpyruvate Dioxygenase (Hppd)-Inhibitor-Resistant Palmer Amaranth (Amaranthus Palmeri S. Watson) In Glyphosate-Resistant Maize, Parminder S. Chahal, Zahoor Ahmad Ganie, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

A Palmer amaranth (Amaranthus palmeri S. Watson) biotype has evolved resistance to photosystem (PS) II- (atrazine) and 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides (mesotrione, tembotrione, and topramezone) in maize seed production field in Nebraska, USA. The objectives of this study were to determine the effect of soil residual pre-emergence (PRE) herbicides followed by (fb) tank-mixture of residual and foliar active post-emergence (POST) herbicides on PS-II- and HPPD-inhibitor-resistant Palmer amaranth control, maize yield, and net economic returns. Field experiments were conducted in a grower’s field infested with PS II- and HPPD-inhibitor-resistant Palmer amaranth near Shickley in Fillmore County, Nebraska, USA in 2015 …

Nebline, January 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Extension 2017 Highlights

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Optimising The Identification Of Causal Variants Across Varying Genetic Architectures In Crops, Chenyong Miao, Jinliang Yang, James C. Schnable

Department of Agronomy and Horticulture: Faculty Publications

Association studies use statistical links between genetic markers and the phenotype variation across many individuals to identify genes controlling variation in the target phenotype. However, this approach, particularly conducted on a genome-wide scale (GWAS), has limited power to identify the genes responsible for variation in traits controlled by complex genetic architectures. In this study, we employ real-world genotype datasets from four crop species with distinct minor allele frequency distributions, population structures and linkage disequilibrium patterns. We demonstrate that different GWAS statistical approaches provide favourable trade-offs between power and accuracy for traits controlled by different types of genetic architectures. FarmCPU provides …

Transcriptional Reprogramming Of Legume Genomes: Perspective And Challenges Associated With Single-Cell And Single Cell-Type Approaches During Nodule Development, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Transcriptomic approaches revealed thousands of genes differentially or specifically expressed during nodulation, a biological process resulting from the symbiosis between leguminous plant roots and rhizobia, atmospheric nitrogen-fixing symbiotic bacteria. Ultimately, nodulation will lead to the development of a new root organ, the nodule. Through functional genomic studies, plant transcriptomes have been used by scientists to reveal plant genes potentially controlling nodulation. However, it is important to acknowledge that the physiology, transcriptomic programs, and biochemical properties of the plant cells involved in nodulation are continuously regulated. They also differ between the different cell-types composing the nodules. To generate a more accurate …