Other Plant Sciences Commons^™

Techniques To Improve The Volume, Texture And Nutritional Quality Of Gluten-Free Bread, Zachary Christman

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

This article highlights the use of soy flour to improve the protein and fiber of gluten free bread. Also, the use of an extrusion process on soybean and corn flour blends improves the volume and texture of the bread. A full listing of the ingredients and method for production of higher quality gluten free bread is included.

Pasture Legumes And Grasses For Saline Land In Western Australia, Department Of Primary Industries And Regional Development, Western Australia

Natural resources factsheets

The productivity of saline and waterlogged land can be markedly increased by using salt tolerant legumes and grasses, in mixtures. The mix is particularly important for increasing the productivity and profitability of saltbush-based pastures. The information is relevant to the south-west agricultural region of Western Australia.

The Department of Primary Industries and Regional Development provides this information on the tolerance and establishment of salt tolerant legumes and grasses to improve the productivity and profitability of saline land.

Saltbushes For Dryland Salinity Management In Western Australia, Department Of Primary Industries And Regional Development, Western Australia

Natural resources factsheets

Saltland pastures always have a range of plant species, even in dense saltbush plantations. Saltbush will often change the watertable and surface soil characteristics enough for other plants to colonise. The understorey plants are often the most productive component of saltbush pastures.

This page provides information about each of the saltbushes that can be used in these mixed salt-tolerant pastures.

The Department of Primary Industries and Regional Development recommends that saltland management is part of a whole farm – or preferable whole catchment – plan.

Tall Wheatgrass (Thinopyrum Ponticum) For Dryland Salinity Management, Department Of Primary Industries And Regional Development, Western Australia

Natural resources factsheets

Tall wheatgrass (Thinopyrum ponticum) has been widely planted in Western Australia on saline land in the southern agricultural areas, on land that shows signs of salt and is not permanently waterlogged.

The information provided here assumes that tall wheatgrass is being grazed effectively by livestock.

Nebline, May 2021

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Building Health Equity in Lincoln: Extension Launches Health Equity Coalition

Recipe of the Month

Intensive Gardening Techniques

Garden Guide Things to Do This Month

Naturally Occurring Elements in Groundwater--Part 3 of a Series — Fluoride

Cash Rent Survey

Preparing for the Inevitable: Japanese Beetles

Challenge Accepted! Children Thrive Outside

Heart of 4-H Volunteer Award: Rusty & Heidi Hanley

4-H Announcements for 4-H'ers and Volunteers

Clover College: June 15–18, 2021

EXTENSION CALENDAR

4-H’ers Qualify for State Speech & PSA Contest

4-H Horse Stampede Results

Nozzle Selection And Adjuvant Impact On The Efficacy Of Glyphosate And Ppo-Inhibiting Herbicide Tank-Mixtures, Jesaelen G. Moraes, Thomas R. Butts, Vitor M. Anunciato, Joe D. Luck, Wesley C. Hoffmann, Ulisses R. Antuniassi, Greg R. Kruger

Department of Agronomy and Horticulture: Faculty Publications

PPO-inhibiting herbicides in combination with glyphosate for postemergence applications is a common approach to manage glyphosate- and ALS-inhibitor-resistant weeds. PPO-inhibitors can reduce glyphosate translocation when applied in tank-mixtures, but adjuvants may be used to overcome this effect. Additionally, optimal droplet size may be affected by tank-mixtures of different herbicides and it can be crucial to herbicide efficacy. Field and greenhouse studies were conducted to investigate the impact of nozzle selection and adjuvants on weed control and interactions when applying PPO-inhibitors (fomesafen or lactofen) alone or in tank-mixture with glyphosate to five weed species using six nozzle types. Ultra-coarse droplets were …

Nebline, April 2021

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Experiential Learning Teaches Skills and More

DIY FitBall: A Fun Fitness Activity

Recipe of the Month

Get Ready to Control Pine Tip Blight

Garden Guide

2021 Prennial Plant of the Year

Naturally Occurring Elements in Groundwater, Part 2 of a Series: Iron and Manganese

No Bull! Examine Your Sires’ Breeding Soundness

Inspiring Rich Conversations through Questions by Sarah Roberts

Become a Community Scientist by Kait Chapman

Hearto f 4-H Volunteer Award: James Blake

4-H Announcements for 4-H'ers and Volunteers

Watch Chicks Hatch on Egg Cam

4-H Achievement Celebration

Extension Calendar

2021 Weed Awareness

Controlling and Managing Phragmites

Take 2, Two …

Comparing A Mixed Model Approach To Traditional Stability Estimators For Mapping Genotype By Environment Interactions And Yield Stability In Soybean [Glycine Max (L.) Merr.], Mary M. Happ, George L. Graef, Haichuan Wang, Reka Howard, Luis Posadas, David L. Hyten

Department of Agronomy and Horticulture: Faculty Publications

Identifying genetic loci associated with yield stability has helped plant breeders and geneticists begin to understand the role and influence of genotype by environment (GxE) interactions in soybean [Glycine max (L.) Merr.] productivity, as well as other crops. Quantifying a genotype’s range of performance across testing locations has been developed over decades with dozens of methodologies available. This includes directly modeling GxE interactions as part of an overall model for yield, as well as methods which generate overall yield “stability” values from multi-environment trial data. Correspondence between these methods as it pertains to the outcomes of genome wide association studies …

Soil Erosion Modelling: A Bibliometric Analysis, Nejc Bezak, Matjǎz Mikǒs, Pasquale Borrelli, Christine Alewell, Dinesh Panday, Panos Panagos, 59 Co-Authors

Department of Agronomy and Horticulture: Faculty Publications

Soil erosion can present a major threat to agriculture due to loss of soil, nutrients, and organic carbon. Therefore, soil erosion modelling is one of the steps used to plan suitable soil protection measures and detect erosion hotspots. A bibliometric analysis of this topic can reveal research patterns and soil erosion modelling characteristics that can help identify steps needed to enhance the research conducted in this field. Therefore, a detailed bibliometric analysis, including investigation of collaboration networks and citation patterns, should be conducted. The updated version of the Global Applications of Soil Erosion Modelling Tracker (GASEMT) database contains information about …

Mental Model-Based Repeated Multifaceted (Mrm) Intervention Design: A Conceptual Framework For Improving Preventive Health Behaviors And Outcomes, Mazbahul G. Ahamad, Fahian Tanin

Department of Agronomy and Horticulture: Faculty Publications

Objective: Field interventions employed to improve preventive health behaviors and outcomes generally use well established approaches; however, recent studies have reported that health education and promotional interventions have little to no impact on health behaviors, especially in low- and middle-income countries. We aimed to develop a conceptual framework to improve intervention designs that would internalize these concerns and limitations.

Results: We identified three major experimental design- and implementation-related concerns associated with mental models, including the balance between the treatment and control groups, the treatment group’s willingness to adopt suggested behaviors, and the type, length, frequency, intensity, and sequence of treatments. …

Grass Bud Responses To Fire In A Semiarid Savanna System, Quinn A. Hiers, Morgan L. Treadwell, Matthew B. Dickinson, Kathleen L. Kavanagh, Alexandra G. Lodge, Heath D. Starns, Doug R. Tolleson, Dirac Twidwell, Carissa L. Wonkka, William E. Rogers

Department of Agronomy and Horticulture: Faculty Publications

Increasingly, land managers have attempted to use extreme prescribed fire as a method to address woody plant encroachment in savanna ecosystems. The effect that these fires have on herbaceous vegetation is poorly understood. We experimentally examined immediate (<24 hr) bud response of two dominant graminoids, a C₃ caespitose grass, Nassella leucotricha, and a C₄ stoloniferous grass, Hilaria belangeri, following fires of varying energy (J/m² ) in a semiarid savanna in the Edwards Plateau ecoregion of Texas. Treatments included high- and low-energy fires determined by contrasting fuel loading and a no burn (control) treatment. Belowground axillary buds were counted and their activities classified to determine immediate effects …

Effects Of Intraguild Interactions On Anticarsia Gemmatalis And Chrysodeixis Includens Larval Fitness And Behavior In Soybean, Sabrina Ongaratto, Edson Ll Baldin, Thomas E. Hunt, Debora G. Montezano, Emily A. Robinson, Maria C. Dos Santos

Department of Agronomy and Horticulture: Faculty Publications

BACKGROUND: Crop pest management requires an understanding of the complex interactions among species that potentially impact crop yield. In soybean, the velvetbean caterpillar, Anticarsia gemmatalis (Hübner), and the soybean looper, Chrysodeixis includens (Walker), are described as key pests, sharing the same feeding guild. We assessed the intraguild interactions of these species under laboratory conditions. Fitness cost study was conducted to examine the influence of competition on insect development. A video tracking system was used to evaluate behavioral parameters during larval interactions in scenarios with and without food availability. RESULTS: In the fitness cost assay, pupal weight was not significantly affected, …

Microbial Community Field Surveys Reveal Abundant Pseudomonas Population In Sorghum Rhizosphere Composed Of Many Closely Related Phylotypes, Dawn Chiniquy, Elle M. Barnes, Jinglie Zhou, Kyle Hartman, Xiaohui Li, Amy Sheflin, Ellen Marsh, Jessica Prenni, Adam M. Deutschbauer, Daniel P. Schachtman, Susannah G. Tringe

Department of Agronomy and Horticulture: Faculty Publications

While the root-associated microbiome is typically less diverse than the surrounding soil due to both plant selection and microbial competition for plant derived resources, it typically retains considerable complexity, harboring many hundreds of distinct bacterial species. Here, we report a time-dependent deviation from this trend in the rhizospheres of field grown sorghum. In this study, 16S rRNA amplicon sequencing was used to determine the impact of nitrogen fertilization on the development of the root-associated microbiomes of 10 sorghum genotypes grown in eastern Nebraska. We observed that early rhizosphere samples exhibit a significant reduction in overall diversity due to a high …

Utility Of Climatic Information Via Combining Ability Models To Improve Genomic Prediction For Yield Within The Genomes To Fields Maize Project, Diego Jarquin, Natalia De Leon, Cinta Romay, Martin Bohn, Edward S. Buckler, Ignacio Ciampitti, Jode Edwards, David Ertl, Sherry Flint-Garcia, Michael A. Gore, Christopher Graham, Candice N. Hirsch, James B. Holland, David Hooker, Shawn M. Kaeppler, Joseph Knoll, Elizabeth C. Lee, Carolyn J. Lawrence-Dill, Jonathan P. Lynch, Stephen P. Moose, Seth C. Murray, Rebecca Nelson, Torbert Rocheford, James C. Schnable, Patrick S. Schnable, Margaret Smith, Nathan Springer, Peter Thomison, Mitch Tuinstra, Randall J. Wisser, Wenwei Xu, Jianming Yu, Aaron Lorenz

Department of Agronomy and Horticulture: Faculty Publications

Genomic prediction provides an efficient alternative to conventional phenotypic selection for developing improved cultivars with desirable characteristics. New and improved methods to genomic prediction are continually being developed that attempt to deal with the integration of data types beyond genomic information. Modern automated weather systems offer the opportunity to capture continuous data on a range of environmental parameters at specific field locations. In principle, this information could characterize training and target environments and enhance predictive ability by incorporating weather characteristics as part of the genotype-by-environment (G×E) interaction component in prediction models. We assessed the usefulness of including weather data variables …

A Microbiome Engineering Framework To Evaluate Rhizobial Symbionts Of Legumes, Kenjiro W. Quides, Hagop S. Atamian

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Background

For well over a century, rhizobia have been recognized as effective biofertilizer options for legume crops. This has led to the widespread use of rhizobial inoculants in agricultural systems, but a recurring issue has emerged: applied rhizobia struggle to provide growth benefits to legume crops. This has largely been attributed to the presence of soil rhizobia and has been termed the ‘rhizobial competition problem.’

Scope

Microbiome engineering has emerged as a methodology to circumvent the rhizobial competition problem by creating legume microbiomes that do not require exogenous rhizobia. However, we highlight an alternative implementation of microbiome engineering that focuses …

Effects Of Foliar Fungicide On Yield, Micronutrients, And Cadmium In Grains From Historical And Modern Hard Winter Wheat Genotypes, Hollman Motta-Romero, Ferdinand Niyongira, Jeffrey D. Boehm Jr, Devin J. Rose

Department of Agronomy and Horticulture: Faculty Publications

Plant breeding and disease management practices have increased the grain yield of hard winter wheat (Triticum aestivum L.) adapted to the Great Plains of the United States during the last century. However, the effect of genetic gains for seed yield and the application of fungicide on the micronutrient and cadmium (Cd) concentration in wheat grains is still unclear. The objectives of this study were to evaluate the effects of fungicide application on the productivity and nutritional quality of wheat cultivars representing 80 years of plant breeding efforts. Field experiments were conducted over two crop years (2017 and 2018) with eighteen …

Nebline, March 2021

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Naturally Occurring Elements in Groundwater - Part 1 of a Series — Calcium and Magnesium

Celebrate National Nutrition Month

RECIPE OF THE MONTH

2021 All-America Selections Vegetable Winners

Starting Seeds Indoors

Garden Guide Things to Do This Month

Planning for Spring Calving Season

Chemigation Trainings

2021 Nebraska On-Farm Research Annual Results Update Meetings

Houseplants: Simple Solutions to Plant Pests

Heart of 4-H Volunteer Award: Whitney Lehn

Meet 2020–2021 4-H Council

4-H Announcements for 4-H'ers and Volunteers

Private Pesticide Applicator Trainings by Zoom

EXTENSION CALENDAR

Upcoming Early Childhood Trainings

Cultivating Hops For Cone Production In Nebraska, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

The hop cone is the primary product of agronomic value when growing Humulus lupulus L. (common hop). Cones are modified stem and leaf structures that protect the female flower cluster that forms chemical compounds important for flavoring beer and other uses. The majority of hops in the United States (~96%) is grown in the states of Washington, Oregon, and Idaho. Hops can be grown in very diverse climates, but it is the climatic consistency of the Pacific NW that provides product consistency and reasoning commercial hops production is prevalent in the region. Hops is a niche crop outside of the …

Novel And Transgressive Salinity Tolerance In Recombinant Inbred Lines Of Rice Created By Physiological Coupling-Uncoupling And Network Rewiring Effects, Isaiah C.M. Pabuayon, Ai Kitazumi, Kevin R. Cushman, Rakesh Kumar Singh, Glenn B. Gregorio, Balpreet Dhatt, Masoud Zabet-Moghaddam, Harkamal Walia, Benildo G. De Los Reyes

Department of Agronomy and Horticulture: Faculty Publications

The phenomenon of transgressive segregation, where a small minority of recombinants are outliers relative to the range of parental phenotypes, is commonly observed in plant breeding populations. While this phenomenon has been attributed to complementation and epistatic effects, the physiological and developmental synergism involved have not been fully illuminated by the QTL mapping approach alone, especially for stress adaptive traits involving highly complex interactions. By systems-level profiling of the IR29 × Pokkali recombinant inbred population of rice, we addressed the hypothesis that novel salinity tolerance phenotypes are created by reconfigured physiological networks due to positive or negative coupling-uncoupling of developmental …

Evaluation Of Soil Moisture Sensing Technologies In Silt Loam And Loamy Sand Soils: Assessment Of Performance, Temperature Sensitivity, And Site- And Sensor-Specific Calibration Functions, K. Sharma, Sibel Irmak, M. S. Kukal, M. C. Vuran, Amit J. Jhala, Xia Qiao

Department of Agronomy and Horticulture: Faculty Publications

Reliable soil moisture information is vital for optimal irrigation management, farm-level agronomic decision-making, hydrologic studies, and cropping systems modeling. A wide range of soil moisture sensing technologies is commercially available, but their performance must be evaluated for diverse conditions of use. In this research, we investigated nine soil moisture sensors based on time-domain reflectometry, capacitance, and electrical resistance principles in production field conditions with two installation orientations, i.e., vertical (V) and horizontal (H), in two soils (silt loam and loamy sand) and two growing seasons (2017 and 2018). Performance parameters deduced from the 2017 datasets revealed that sensor type and …

Nebline, February 2021

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Staying active in winter

Nebraska Extension’s Physical Activity Resources

At-Home Workout: No Equipment Needed

Recipe of the Month

2021 Flower All-America Selections Winners

Garden Guide Things to Do This Month

ProHort Lawn & Landscape Update

Know Your Bugs Before You Treat: Bed vs. Bat

More Than Counting: Incorporating Math Into Daily Interactions With Preschoolers

Pesticide Applicator Trainings

Total Coliform Bacteria in Drinking Water

HEART OF 4-H Volunteer Award, Laura Cook

Meet 2020–2021 4-H Teen Council

4-H Club Gives Youth With Special Needs Opportunity to Show Lambs

Pick-A-Pig Club Accepting New Members

Companion Animal Webinar Series

4-H Rabbit Show, March 20 …

Corn Residue Baling And Grazing Impacts On Corn Yield Under Irrigated Conservation Tillage Systems, Sabrina J. Ruis, Charles Burr, Humberto Blanco-Canqui, Brian Olson, Mark Reiman, Daran Rudnick, Johnna Baller, Kathryn J. Hanford

Department of Agronomy and Horticulture: Faculty Publications

Crop residue grazing or baling is common in the western Corn Belt. However, its impacts on subsequent crop yields under different irrigation levels and tillage systems are unclear. We investigated the impacts of corn (Zea mays L.) residue baling and cattle grazing on soil compaction, water content, and corn yield under full and limited irrigated no-till in Nebraska during three years. In Years 2 and 3, an additional tillage treatment (strip till) was implemented to evaluate its effects on grain yield under the above treatments. Residue removal effects on compaction and water content did not vary with irrigation level. …

Seedextractor: An Open-Source Gui For Seed Image Analysis, Feiyu Zhu, Puneet Paul, Waseem Hussain, Kyle Wallman, Balpreet K. Dhatt, Jaspreet Sandhu, Larissa Irvin, Gota Morota, Hongfeng Yu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Accurate measurement of seed size parameters is essential for both breeding efforts aimed at enhancing yields and basic research focused on discovering genetic components that regulate seed size. To address this need, we have developed an open source graphical user interface (GUI) software, SeedExtractor that determines seed size and shape (including area, perimeter, length, width, circularity, and centroid), and seed color with capability to process a large number of images in a time-efficient manner. In this context, our application takes 2 s for analyzing an image, i.e., significantly less compared to the other tools. As this software is open-source, it …

Soil Properties Limiting Vegetation Establishment Along Roadsides, Shad D. Mills, Martha Mamo, Sabrina J. Ruis, Humberto Blanco-Canqui, Walter Schacht, Tala Awada, Xu Li, Pamela Sutton

Department of Agronomy and Horticulture: Faculty Publications

Roadside vegetation provides a multitude of ecosystem services, including pollutant remediation, runoff reduction, wildlife habitat, and aesthetic scenery. Establishment of permanent vegetation along paved roads after construction can be challenging, particularly within 1 m of the pavement. Adverse soil conditions could be one of the leading factors limiting roadside vegetation growth. In this study, we assessed soil physical and chemical properties along a transect perpendicular to the road at six microtopographic positions (road edge, shoulder, side slope, ditch, backslope, and field edge) along two highway segments near Beaver Crossing and Sargent, NE. At the Beaver Crossing site, Na concentration was …

Intervention Of Climate‐Smart Practices In Wheat Under Rice‐ Wheat Cropping System In Nepal, Jamma Jaya Gairhe, Mandeep Adhikari, Deepak Ghimire, Arun Kjatri-Chhetri, Dinesh Panday

Department of Agronomy and Horticulture: Faculty Publications

Besides a proper agronomic management followed by Nepalese farmers, wheat (Triticum aestivum L.) production has been severely affected by changing climate. There are many interventions, including climate‐smart practices, to cope with this situation and possibly enhance crop and soil productivity. Field experiments were set up in a randomized complete block design with six treatments (TRT) with four replications in three locations (LOC) during wheat‐growing seasons in Nepal from 2014 to 2016. Treatments included (i) Controlled Practice (CP), (ii) Improved Low (IL), (iii) Improved High (IH), (iv) Climate Smart Agriculture Low (CSAL), (v) Climate Smart Agriculture Medium (CSAM), and (vi) Climate …

A Bumper Crop Of Snps In Soybean Through High-Density Genotyping-By-Sequencing (Hd-Gbs), Davoud Torkamaneh, Jerome Laroche, Brian Boyle, David L. Hyten, Fancois Belzile

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.

Genome-Wide Discovery Of Natural Variation In Pre-Mrna Splicing And Prioritising Causal Alternative Splicing To Salt Stress Response In Rice, Huihui Yu, Qian Du, Malachy Campbell, Bin Yu, Harkamal Walia, Chi Zhang

Department of Agronomy and Horticulture: Faculty Publications

Pre-mRNA splicing is an essential step for the regulation of gene expression. In order to specifically capture splicing variants in plants for genome-wide association studies (GWAS), we developed a software tool to quantify and visualise Variations of Splicing in Population (VASP). VASP can quantify splicing variants from short-read RNA-seq datasets and discover genotype-specific splicing (GSS) events, which can be used to prioritise causal pre-mRNA splicing events in GWAS. We applied our method to an RNA-seq dataset with 328 samples from 82 genotypes from a rice diversity panel exposed to optimal and saline growing conditions. In total, 764 significant GSS events …

Gwas Revealed Effect Of Genotype × Environment Interactions For Grain Yield Of Nebraska Winter Wheat, Shamseldeen Eltaher, P Stephen Baenziger, Vikas Belamkar, Hamdy A. Emara, Ahmed A. Nower, Khaled F.M. Salem, Ahmad M. Alqudah, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Background: Improving grain yield in cereals especially in wheat is a main objective for plant breeders. One of the main constrains for improving this trait is the G × E interaction (GEI) which affects the performance of wheat genotypes in different environments. Selecting high yielding genotypes that can be used for a target set of environments is needed. Phenotypic selection can be misleading due to the environmental conditions. Incorporating information from phenotypic and genomic analyses can be useful in selecting the higher yielding genotypes for a group of environments. Results: A set of 270 F_3:6 wheat genotypes in the …

Nebline, January 2021

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

2020 Extension Highlight Highlights Responding as Local Needs Changed

Helping People Solve Real-World Problems

20,311 Youth Engaged in 4-H Programs

Make Your New Year’s Resolutions Stick

Recipe of the Month

Crop Production Clinics, Jan. 7, 14, and 21

Pesticide Applicator Trainings

Extension’s 2021 Master Gardener Trainings, Two Sites Available

Upcoming Green Industry Conferences

Garden Guide Things to do This Month

HEART OF 4-H VOLUNTEER AWARD: Emalea Dean

PASE Results

4-H Thanks Sponsors

4-H Announcements for 4-H’ers and Volunteers

4-H Thanks Volunteers

EXTENSION CALENDAR

EXTENSION NEWS

Seed-Shattering Phenology At Soybean Harvest Of Economically Important Weeds In Multiple Regions Of The United States. Part 2: Grass Species, Lauren M. Schwartz-Lazaro, Lovreet S. Shergill, Jeffrey A. Evans, Muthukumar V. Bagavathiannan, Shawn C. Beam, Mandy D. Bish, Jason A. Bond, Kevin W. Bradley, William S. Curran, Adam S. Davis, Wesley J. Everman, Michael L. Flessner, Steven C. Haring, Nicholas R. Jordan, Nicholas E. Korres, John L. Lindquist, Jason K. Norsworthy, Tameka L. Sanders, Larry E. Steckel, Mark J. Vangessel, Blake Young, Steven B. Mirsky

Department of Agronomy and Horticulture: Faculty Publications

Seed shatter is an important weediness trait on which the efficacy of harvest weed seed control (HWSC) depends. The level of seed shatter in a species is likely influenced by agroecological and environmental factors. In 2016 and 2017, we assessed seed shatter of eight economically important grass weed species in soybean [Glycine max (L.) Merr.] from crop physiological maturity to 4 wk after maturity at multiple sites spread across 11 states in the southern, northern, and mid-Atlantic United States. From soybean maturity to 4 wk after maturity, cumulative percent seed shatter was lowest in the southern U.S. regions and increased …