Horticulture Commons^™

Estimating Economic Minimums Of Mowing, Fertilizing, And Irrigating Turfgrass, Douglas J. Soldat, James T. Brosnan, Ambika Chandra, Roch E. Gaussoin, Alec Kowalewski, Bernd Leinauer, Frank S. Rossi, John C. Stier, J. Bryan Unruh

Department of Agronomy and Horticulture: Faculty Publications

The public health crisis and economic recession caused by the COVID-19 pandemic have forced turfgrass industry professionals to re-evaluate standard practices. Minimum costs required to fertilize, irrigate, and mow turfgrasses can be roughly estimated using climate data, turfgrass physiology information, and resource costs. Although the actual minimum costs vary situationally and regionally, mowing golf putting greens optimally requires about US$34 per acre per month, whereas other turfgrass areas cost less than US$11 per acre per growing month. Fertilizer applications to turfgrass cost US$22 or less per acre per growing month. Irrigation costs (water and electricity for pumping) vary widely, with …

Continuous Cropping Alters Multiple Biotic And Abiotic Indicators Of Soil Health, Pervaiz Ahmad, Javed Iqbal, Qingming Zhang, Dima Chen, Hui Wei, Muhammad Saleem

Department of Agronomy and Horticulture: Faculty Publications

The continuous cropping (CC) of major agricultural, horticultural, and industrial crops is an established practice worldwide, though it has significant soil health-related concerns. However, a combined review of the effects of CC on soil health indicators, in particular omics ones, remains missing. The CC may negatively impact multiple biotic and abiotic indicators of soil health, fertility, and crop yield. It could potentially alter the soil biotic indicators, which include but are not limited to the composition, abundance, diversity, and functioning of soil micro- and macro-organisms, microbial networks, enzyme activities, and soil food web interactions. Moreover, it could also alter various …

Use Of Family Structure Information In Interaction With Environments For Leveraging Genomic Prediction Models, Reyna Persa, Hiroyoshi Iwata, Diego Jarquin

Department of Agronomy and Horticulture: Faculty Publications

The characterization of genomes with great detail offered by the modern genotyping platforms have opened a venue for accurately predicting the genotype-by-environment interaction (GE) effects of untested genotypes in different environmental conditions. Already developed statistical models have shown the advantages of including the GE interaction component in the prediction context using molecular markers, pedigree, or both. In order to leverage the family information of highly structured populations when pedigree data is not available, we developed a model that uses the family membership instead. The proposed model extends the reaction norm model by including the interaction between families and environments (FE). …

Coal Char Affects Soil Ph To Reduce Ammonia Volatilization From Sandy Loam Soil, Dinesh Panday, Maysoon M. Mikha, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Ammonia (NH3) volatilization loss adversely affects N availability in soil-plant systems, reduces crop yield, and negatively impacts environment. Char (coal combus- tion residue), which contains up to 293 g kg−1 total C by weight, has been shown to reduce NH3 volatilization due to its considerably high surface area and cation exchange capacity. The NH3 loss can be greatly affected by a shift in soil pH or urea hydrolysis. A 21-d laboratory study was conducted to evaluate the effects of char on soil pH, N transformations, and subsequent NH3 volatilization in sandy loam soil. Two char rates (0 and 13.4 Mg …

Isoseq Transcriptome Assembly Of C3 Panicoid Grasses Provides Tools To Study Evolutionary Change In The Panicoideae, Daniel S. Carvalho, Aime Nishimwe, James Schnable

Department of Agronomy and Horticulture: Faculty Publications

The number of plant species with genomic and transcriptomic data has been increasing rapidly. The grasses—Poaceae—have been well represented among species with published reference genomes. However, as a result the genomes of wild grasses are less frequently targeted by sequencing efforts. Sequence data from wild relatives of crop species in the grasses can aid the study of domestication, gene discovery for breeding and crop improvement, and improve our understanding of the evolution of C₄ photosynthesis. Here, we used long-read sequencing technology to characterize the transcriptomes of three C₃ panicoid grass species: Dichanthelium oligosanthes, Chasmanthium laxum, and …

The Lateral Root Density Gene Regulates Root Growth During Water Stress In Wheat, Dante F. Placido, Jaspreet Sandhu, Shirley Sato, Natalya Nersesian, Truyen Quach, Thomas Clemente, Paul Staswick, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Drought stress is the major limiting factor in agriculture. Wheat, which is the most widely grown crop in the world, is predominantly cultivated in drought-prone rainfed environments. Since roots play a critical role in water uptake, root response to water limitations is an important component for enhancing wheat adaptation. In an effort to discover novel genetic sources for improving wheat adaptation, we characterized a wheat translocation line with a chromosomal segment from Agropyron elongatum, a wild relative of wheat, which unlike common wheat maintains root growth under limited-water conditions. By exploring the root transcriptome data, we found that reduced …

Adjusting Corn Nitrogen Management By Including A Mineralizable-Nitrogen Test With The Preplant And Presidedress Nitrate Tests, Jason Clark, Fabián G. Fernández, Kristen S. Veum, James J. Camberato, Paul R. Carter, Richard Ferguson, David W. Franzen, Daniel E. Kaiser, Newell R. Kitchen, C. A.M. Laboski, Emerson Nafziger, Carl J. Rosen, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

The anaerobic potentially mineralizable N (PMN) test combined with the preplant (PPNT) and presidedress (PSNT) nitrate tests may improve corn (Zea mays L.) N fertilization predictions. Forty-nine corn N response experiments (mostly corn following soybean [Glycine max (L.) Merr.]) were conducted in the U.S. Midwest from 2014–2016 to evaluate the ability of the PPNT and PSNT to predict corn relative yield (RY) and N fertilizer over- and under-application rates when adjusted by PMN. Before planting and N fertilization, PPNT (0–30, 30–60, and 60–90 cm) and PMN (0–30 cm) samples were obtained. In-season soil samples were obtained at the …

Relating Four-Day Soil Respiration To Corn Nitrogen Fertilizer Needs Across 49 U.S. Midwest Fields, G. M. Bean, Newell R. Kitchen, Kristen S. Veum, James J. Camberato, Richard Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A.M. Laboski, Emerson Nafziger, John E. Sawyer, Matt Yost

Department of Agronomy and Horticulture: Faculty Publications

Soil microbes drive biological functions thatmediate chemical and physical processes necessary for plants to sustain growth. Laboratory soil respiration has been proposed as one universal soil health indicator representing these functions, potentially informing crop and soil management decisions. Research is needed to test the premise that soil respiration is helpful for profitable in-season nitrogen (N) rate management decisions in corn (Zea mays L.). The objective of this research was two-fold: (i) determine if the amount of N applied at the time of planting effected soil respiration, and (ii) evaluate the relationship of soil respiration to corn yield response to …

Adding A Late Fall Application Of Proxy (Ethephon) Before Two Traditional Spring Applications Improves Seedhead Control Of Annual Bluegrass, Zachary Reicher, Matthew Sousek, Aaron J. Patton, Adam Van Dyke, William C. Kreuser, John C. Inguagiato, Kevin M. Miele, John Brewer, Shawn D. Askew, Aaron Hathaway, Thomas A. Nikolai, Alec Kowalewski, Brian Mcdonald

Department of Agronomy and Horticulture: Faculty Publications

Annual bluegrass (ABG) (Poa annua L.) is a prolific seed producer in the spring on golf courses that in turn decreases aesthetic quality and trueness of ball roll on coolseason putting greens. Proxy (ethephon) applied twice in the spring after green-up is the current industry standard after the loss of Embark (mefluidide) from the turf and ornamental market. However, plant growth regulators including Proxy have been used for years to help suppressABGseedheads with inconsistent success. The primary objective of this study was to determine if ABG seedhead suppression is improved by adding a late fall application of Proxy to …

Allelic Variation In Rice Fertilization Independent Endosperm 1 Contributes To Grain Width Under High Night Temperature Stress, Balpreet K. Dhatt, Puneet Paul, Jaspreet Sandhu, Waseem Hussain, Larissa Irvin, Feiyu Zhu, Maria Arlene Adviento-Borbe, Argelia Lorence, Paul Staswick, Hongfeng Yu, Gota Morota, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

A higher minimum (night-time) temperature is considered a greater limiting factor for reduced rice yield than a similar increase in maximum (daytime) temperature. While the physiological impact of high night temperature (HNT) has been studied, the genetic and molecular basis of HNT stress response remains unexplored.

We examined the phenotypic variation for mature grain size (length and width) in a diverse set of rice accessions under HNT stress. Genome-wide association analysis identified several HNT-specific loci regulating grain size as well as loci that are common for optimal and HNT stress conditions.

A novel locus contributing to grain width under HNT …

Leveraging Genome-Enabled Growth Models To Study Shoot Growth Responses To Water Deficit In Rice, Malachy T. Campbell, Alexandre Grondin, Harkamal Walia, Gota Morota

Department of Agronomy and Horticulture: Faculty Publications

lucidating genotype-by-environment interactions and partitioning its contribution to phenotypic variation remains a challenge for plant scientists. We propose a framework that utilizes genome-wide markers to model genotype-specific shoot growth trajectories as a function of time and soil water availability. A rice diversity panel was phenotyped daily for 21 d using an automated, high-throughput image-based, phenotyping platform that enabled estimation of daily shoot biomass and soil water content. Using these data, we modeled shoot growth as a function of time and soil water content, and were able to determine the time point where an inflection in the growth trajectory occurred. We …

Voxel Carving-Based 3d Reconstruction Of Sorghum Identifies Genetic Determinants Of Light Interception Efficiency, Mathieu Gaillard, Chenyong Miao, James Schnable, Bedrich Benes

Department of Agronomy and Horticulture: Faculty Publications

Changes in canopy architecture traits have been shown to contribute to yield increases. Optimizing both light interception and light interception efficiency of agricultural crop canopies will be essential to meeting the growing food needs. Canopy architecture is inherently three-dimensional (3D), but many approaches to measuring canopy architecture component traits treat the canopy as a two-dimensional (2D) structure to make large scale measurement, selective breeding, and gene identification logistically feasible. We develop a high throughput voxel carving strategy to reconstruct 3D representations of sorghum from a small number of RGB photos. Our approach builds on the voxel carving algorithm to allow …

Genome-Wide Identification And Analysis Of Heterotic Loci In Three Maize Hybrids, Hongjun Liu, Qin Wang, Mengjiao Chen, Yahui Ding, Xuerong Yang, Jie Liu, Xiaohan Li, Congcong Zhou, Qilin Tian, Yiqi Lu, Danlin Fan, Junpeng Shi, Lin Zhang, Congbin Kang, Mingfei Mingfei Sun, Fangyuan Li, Yujian Wu, Yongzhong Zhang, Baoshen Liu, Xiang Yu Zhao, Qi Feng, Jinliang Yang, Bin Han, Jinsheng Lai, Xian Sheng Zhang, Xuehui Huang

Department of Agronomy and Horticulture: Faculty Publications

Heterosis, or hybrid vigour, is a predominant phenomenon in plant genetics, serving as the basis of crop hybrid breeding, but the causative loci and genes underlying heterosis remain unclear in many crops. Here, we present a large-scale genetic analysis using 5360 offsprings from three elite maize hybrids, which identifies 628 loci underlying 19 yield-related traits with relatively high mapping resolutions. Heterotic pattern investigations of the 628 loci show that numerous loci, mostly with complete–incomplete dominance (the major one) or overdominance effects (the secondary one) for heterozygous genotypes and nearly equal proportion of advantageous alleles from both parental lines, are the …

Do Coffee Agroforestry Systems Always Improve Soil Carbon Stocks Deeper In The Soil?—A Case Study From Turrialba, Costa Rica, Nilovna Chatterjee, P. K. Ramachandran Nair, Vimala D. Nair, Abhishek Bhattacharjee, Elias De Melo Virginio Filho, Rheinhold G. Muschler, Martin R.A. Noponen

Department of Agronomy and Horticulture: Faculty Publications

Shaded perennial agroforestry systems (AFS) are regarded as desirable land‐use practices that improve soil carbon sequestration. However, most studies assume a positive correlation between above ground and below ground carbon without considering the effect of past and current land management, textural variations (silt and clay percentage), and such other site‐specific factors that have a major influence on the extent of soil C sequestration. We assessed SOC stock at various depths (0–10, 10–30, 30–60, and 60–100 cm) in shaded perennial coffee (Coffea arabica L.) AFS in a 17‐ year‐old experimental field at the Centro Agronómico Tropical de Investigación y Enseñanza, (9°53′44′′ …

Pi‑Plat: A High‑Resolution Image‑Based 3d Reconstruction Method To Estimate Growth Dynamics Of Rice Inflorescence Traits, Jaspreet Sandhu, Feiyu Zhu, Puneet Paul, Tian Gao, Balpreet K. Dhatt, Yufeng Ge, Paul E. Staswick, Hongfeng Yu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Background: Recent advances in image-based plant phenotyping have improved our capability to study vegetative stage growth dynamics. However, more complex agronomic traits such as inflorescence architecture (IA), which predominantly contributes to grain crop yield are more challenging to quantify and hence are relatively less explored. Previous efforts to estimate inflorescence-related traits using image-based phenotyping have been limited to destructive end-point measurements. Development of non-destructive inflorescence phenotyping platforms could accelerate the discovery of the phenotypic variation with respect to inflorescence dynamics and mapping of the underlying genes regulating critical yield components.

Results: The major objective of this study is to evaluate …

Divergent Phenotypic Response Of Rice Accessions To Transient Heat Stress During Early Seed Development, Puneet Paul, Balpreet K. Dhatt, Jaspreet Sandhu, Waseem Hussain, Larissa Irvin, Gota Morota, Paul E. Staswick, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Increasing global surface temperatures is posing a major food security challenge. Part of the solution to address this problem is to improve crop heat resilience, especially during grain development, along with agronomic decisions such as shift in planting time and increasing crop diversification. Rice is a major food crop consumed by more than 3 billion people. For rice, thermal sensitivity of reproductive development and grain filling is well-documented, while knowledge concerning the impact of heat stress (HS) on early seed development is limited. Here, we aim to study the phenotypic variation in a set of diverse rice accessions for elucidating …

Deep Kernel And Deep Learning For Genome-Based Prediction Of Single Traits In Multienvironment Breeding Trials, José Crossa, Johannes W.R. Martini, Daniel Gianola, Paulino Pérez-Rodríguez, Diego Jarquin, Philomin Juliana, Osval Antonio Montesinos López, Jaime Cuevas

Department of Agronomy and Horticulture: Faculty Publications

Deep learning (DL) is a promising method for genomic-enabled prediction. However, the implementation of DL is difficult because many hyperparameters (number of hidden layers, number of neurons, learning rate, number of epochs, batch size, etc.) need to be tuned. For this reason, deep kernel methods, which only require defining the number of layers, may be an attractive alternative. Deep kernel methods emulate DL models with a large number of neurons, but are defined by relatively easily computed covariance matrices. In this research, we compared the genome-based prediction of DL to a deep kernel (arc-cosine kernel, AK), to the commonly used …

Plant Hormones Differentially Control The Sub-Cellular Localization Of Plasma Membrane Microdomains During The Early Stage Of Soybean Nodulation, Zhenzhen Qiao, Prince Zogli, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Phytohormones regulate the mutualistic symbiotic interaction between legumes and rhizobia, nitrogen-fixing soil bacteria, notably by controlling the formation of the infection thread in the root hair (RH). At the cellular level, the formation of the infection thread is promoted by the translocation of plasma membrane microdomains at the tip of the RH. We hypothesize that phytohormones regulate the translocation of plasma membrane microdomains to regulate infection thread formation. Accordingly, we treated with hormone and hormone inhibitors transgenic soybean roots expressing fusions between the Green Fluorescent Protein (GFP) and GmFWL1 or GmFLOT2/4, two microdomain-associated proteins translocated at the tip of the …

Evaluation Of A Global Spring Wheat Panel For Stripe Rust: Resistance Loci Validation And Novel Resources Identification, Ibrahim Elbasyoni, Walid M. El-Orabey, Sabah Morsy, P. S. Baenziger, Zakaria Al Ajlouni4, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Stripe rust (incited by Puccinia striiformis f. sp. tritici) is airborne wheat (Triticum aestivum L.) disease with dynamic virulence evolution. Thus, anticipatory and continued screening in hotspot regions is crucial to identify new pathotypes and integrate new resistance resources to prevent potential disease epidemics. A global wheat panel consisting of 882 landraces and 912 improved accessions was evaluated in two locations in Egypt during 2016 and 2017. Five prevalent and aggressive pathotypes of stripe rust were used to inoculate the accessions during the two growing seasons and two locations under field conditions. The objectives were to evaluate the panel for …

Metabolic Dynamics Of Developing Rice Seeds Under High Night-Time Temperature Stress, Balpreet K. Dhatt, Nathan Abshire, Puneet Paul, Kalani Hasanthika, Jaspreet Sandhu, Qi Zhang, Toshihiro Obata, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

High temperature stress during rice reproductive development results in yield losses. Reduced grain yield and grain quality has been associated with high temperature stress, and specifically with high night-time temperatures (HNT). Characterizing the impact of HNT on the phenotypic and metabolic status of developing rice seeds can provide insights into the mechanisms involved in yield and quality decline. Here, we examined the impact of warmer nights on the morphology and metabolome during early seed development in six diverse rice accessions. Seed size was sensitive to HNT in four of the six genotypes, while seed fertility and seed weight were unaffected. …

Nebline, November/December 2019

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

4-H Horse Program Grows Youth for Life

Thanksgiving Food Safety

Recipe of the Month

Successful Farmer Series Starts Jan. 10

Produce Safety Workshop, Nov. 19

Pesticide Applicator Trainings

Upcoming Green Industry Conferences

A New Can of Worms: Asian Jumping Worms

Use Caution to Avoid Deer-Vehicle Collisions

Reuse Your Fresh Cut Christmas Tree for Wildlife

Gifts For The Gardener

Garden Guide: Things to Do This Month

High-Quality Child Care Depends on Effective Family Engagement

Heart of 4-H Volunteer Award: Beverlee Keller

Heart of 4-H Volunteer Award: Dave Hattan

4-H Video Companion Animal Showmanship Contest Results

Outgoing 4-H Council Members

2019 4-H …

Ghd8 Controls Rice Photoperiod Sensitivity By Forming A Complex That Interacts With Ghd7, Peng Wang, Rong Gong, Ying Yang, Sibin Yu

Department of Agronomy and Horticulture: Faculty Publications

Background: Flowering time is one of the most important agronomic characteristics that ultimately determine yield potential and eco-geographical adaptation in crops. Ghd8 and Ghd7, two major flowering genes, have similar functions and large pleiotropic effects in controlling the heading date, plant height and grain yield of rice. However, these gene interactions at the genetic and molecular levels have not been determined to date.

Results: In this study, we investigated the genetic interaction between Ghd8 and Ghd7 by using a set of near-isogenic lines and a panel of natural germplasm accessions in rice. We found that Ghd8 affected multiple agronomic traits …

Application Of Autofluorescence For Confocal Microscopy To Aid In Archaeoparasitological Analyses, Johnica Jo Morrow, Christian Elowsky

Department of Agronomy and Horticulture: Faculty Publications

Confocal laser scanning microscopy (CLSM) was used to examine archaeoparasitological specimens from coprolites associated with La Cueva de los Muertos Chiquitos (CMC) located near present-day Durango, Mexico. The eggs for 4 different types of parasites recovered from CMC coprolites were imaged using CLSM to assist with identification efforts. While some of the parasite eggs recovered from CMC coprolites were readily identified using standard light microscopy (LM), CLSM provided useful data for more challenging identifications by highlighting subtle morphological features and enhancing visualization of parasite egg anatomy. While other advanced microscopy techniques, such as scanning electron microscopy (SEM), may also detect …

Nebline, October 2019

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Groundwater Protection: It’s Up to Everyone

The Nutritional Power of Pumpkins

Recipe of the Month

Sulfur Deficiency in Corn

Planting Garlic in the Fall Vegetable Garden

Composting Demonstrations

I Spy a Spider: Common Spiders Around the Home

Invasive Species: American Bullfrog

Plant Spring Bulbs for Pollinators

Garden Guide: Things to Do This Month

Become a Master Gardener

Chatting With Babies Helps Brain Development

Heart of 4-H Volunteer Award: Kelsey Ebke

4-H Golf Tournament Fundraiser, Sept. 29

4-H Specialty Clubs Invite New Members

4-H Announcements for 4-H'ers and Volunteers

State Fair 4-H Top Results

Lancaster County 4-H Quilt of Valor Presented …

Canavalia And Dolichos Extracts For Sustainable Pest Biocontrol And Plant Nutrition Improvement In El Salvador, Carlos Martinez

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Botanical repellents and pesticides are now being rediscovered as new tools for integrated pest management in order to reduce the use of toxic chemicals in crop production. Canavalia gladiata and Dolichos lablab are two Fabaceae very well adapted to farmlands of El Salvador, effective as living barriers and mostly as cover crops, however, they are not yet very well disseminated. This document describes the potential for using the liquid extracts and the dry flour of raw seeds of those plants for economic benefit and practical convenience for pest management in Salvadorian agriculture under field conditions. Seed extracts were useful when …

Nebline, September 2019

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Read for Resilience Program Helps Young Children Cope After a Disaster

Breakfast — Don’t Leave Home Without It!

Recipe of the Month

Grazing Calves on Annual Forages

Sheet Composting Requires No Turning

Composting Demonstrations

Prepared, Not Scared. Be Ready for Disasters

Plant Asters for Fall Color

Garden Guide: Things to Do This Month

The Lowdown on Lyme Disease

Heart of 4-H Volunteer Award: Brenda Nepper

4-H Teen Council Invites New Members

Support 4-H by Dining at Chipotle on O Street, Sept. 16

New Horticulture 4-H Club Meeting, Sept. 19

4-H Golf Tournament Fundraiser, Sept. 29

Interested in Joining 4-H? Come …

Nebline, August 2019

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Kids Eat Right Month

Food Safety Tips for Electric Multi-Cookers

Recipe of the Month

Soybean Management Field Days

Landlord–Tenant Cash Rent Workshop, Aug. 21

Fall is Best for Control of Tough Weeds

Free Water Screening for Nitrates

Include Pets in Your Emergency Plan

Going Batty: Bugs on Bats

Make Your Yard a Greener Place

Garden Guide: Things to Do This Month

Tips for a Fun and Educational Day at the Fair

Upcoming Early Childhood Trainings

Heart of 4-H Volunteer Award: Susan Holland

4-H’ers Test Family and Consumer Science and Entrepreneurship Skills at Life Challenge

4-H’ers Test Their Knowledge of Animals …

An Integrated Genomics And Phenomics Approach To Study The Evolution Of C4 Photosynthesis, Daniel Santana De Carvalho

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The C4 photosynthetic pathway was first described over 50 years ago. Today, it is known that C4 evolved independently > 60 in plant lineages, which involves understanding not only the genetic, but also the metabolic features and differences involved in this process. Also, several adaptations are involved in the evolution of this type of photosynthesis, for example: changes in leaf anatomy and the evolution of kranz anatomy, physiology and metabolic pathways. In order to further investigate this pathway, different technologies and methods have been developed to unravel genes involved in C4 photosynthesis. With the advances in molecular biology and bioinformatics tools …

Rust And Viral Mosaic Diseases In Biofuel Switchgrass, Anthony A. Muhle

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Switchgrass (Panicum virgatum L.) is a perennial warm-season monocot that is indigenous to locations in North America east of the Rocky Mountains, and is considered a model grass for biofuel feedstock production. As switchgrass production increases, diseases pose a potential threat to biomass production and ethanol extraction. The two predominant switchgrass diseases in Nebraska are rust caused by Puccinia spp. and a viral mosaic disease caused by Panicum mosaic virus (PMV) and its associated Satellite panicum mosaic virus (SPMV). In this thesis, one study determined how SPMV affects PMV infection and systemic spread in two populations of switchgrass at different …

Hormonal Signaling Induced In Soybean By Lysobacter Enzymogenes Strain C3, Jessica C. Walnut

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The biological control bacterium Lysobacter enzymogenes strain C3 has been shown to suppress fungal diseases by producing a suite of lytic enzymes and antimicrobial secondary metabolites. Previous studies have found that C3, when applied to grass and cereal plants, also is capable of inducing local and systemic resistance against fungal pathogens. It is unknown, however, whether the bacterium has the ability to induce resistance in dicots and what signaling pathways are involved. This study assessed the ability of C3 to trigger local and systemic induced resistance responses in soybean (Glycine max ‘Williams82’) by analyzing relative expression of salicylic acid …