Botany Commons^™

Observing Gravitropism In Brassica Rapa Roots, Trang Ly, Maya O'Callagham, Lan Poeschl, Adil Saeed, George Zorn

Undergraduate Research Posters

The objective of this experiment is to observe the effect of gravitropism on the orientation of Brassica rapa (B. rapa) roots. Specifically, amyloplasts that reside at the bottom of root tips act as gravity sensors. As plants are disturbed from upright orientation, roots automatically grow according to the downward pull of gravity. It is hypothesized that as the angle of rotation increases, the angle of root growth is inversely proportional. Five plants containing four seeds of B. rapa were grown over the course of 10 days. Each plates, aside from the control, were rotated once 90°, 80°, and 270° to …

Field Validation Of A Farmer Supplied Data Approach To Close Soybean Yield Gaps In The Us North Central Region, José F. Andrade, Spyridon Mourtzinis, Juan I. Rattalino Edreira, Shawn P. Conley, John Gaska, Herman J. Kandel, Laura E. Lindsey, Seth Naeve, Scott Nelson, Maninder P. Singhi, Laura J. Thompson, James E. Specht, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

CONTEXT: Producer-reported data can be used to identify suites of management practices that lead to higher yield and profit. However, a rigorous validation of the approach in relation to its potential impact on farmer yield and profit is lacking.

OBJECTIVE: This study aimed to validate a producer-data approach on its capability to guide on-farm evaluation of management practices with greatest potential for increasing producer yield and profit. We show proof of concept using soybean in the North Central US region as a case study.

METHODS: We used a combination of regression tree analysis and a spatial framework to determine practices …

A New Perspective When Examining Maize Fertilizer Nitrogen Use Efficiency, Incrementally, Newell R. Kitchen, Curtis J. Ransom, James S. Schepers, Jerry L. Hatfield, Raymond Massey, Scott T. Drummond

Department of Agronomy and Horticulture: Faculty Publications

For maize (Zea mays L.), nitrogen (N) fertilizer use is often summarized from field to global scales using average N use efficiency (NUE). But expressing NUE as averages is misleading because grain increase to added N diminishes near optimal yield. Thus, environmental risks increase as economic benefits decrease. Here, we use empirical datasets obtained in North America of maize grain yield response to N fertilizer (n = 189) to create and interpret incremental NUE (iNUE), or the change in NUE with change in N fertilization. We show for those last units of N applied to reach economic optimal N …

Inactivation Of The Entire Arabidopsis Group Ii Gh3s Confers Tolerance To Salinity And Water Deficit, Rubén Casanova-Sáez, Eduardo Mateo-Bonmatí, Jan Šimura, Aleš Pěnčík, Ondřej Novák, Paul Staswick, Karin Ljung

Department of Agronomy and Horticulture: Faculty Publications

Indole-3-acetic acid (IAA) controls a plethora of developmental processes. Thus, regulation of its concentration is of great relevance for plant performance. Cellular IAA concentration depends on its transport, biosynthesis and the various pathways for IAA inactivation, including oxidation and conjugation.

Group II members of the GRETCHEN HAGEN 3 (GH3) gene family code for acyl acid amido synthetases catalysing the conjugation of IAA to amino acids. However, the high degree of functional redundancy among them has hampered thorough analysis of their roles in plant development.

In this work, we generated an Arabidopsis gh3.1,2,3,4,5,6,9,17 (gh3oct) mutant to knock out the group II …

Root Exudates Impact Plant Performance Under Abiotic Stress, Yen Ning Chai, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Plant root exudates serve pivotal roles in supporting plant development and interactions with the physicochemical and biological factors in the rhizosphere. Under stress conditions, root exudation is involved in enhancing plant resource-use efficiency and facilitating the crosstalk between plant and soil microbes to ameliorate stress. Although there are a large number of root exudates that remain to be characterized, recent technological advancements have allowed for the function of many exudate compounds to be elucidated. In this review, we discuss current knowledge about the key root exudates that modulate plant resource-use efficiency under various abiotic stresses including drought, aluminum toxicity, phosphorus, …

Morpho-Physiological Diversity Of Barley (Hordeum Vulgare L.) Germplasm For Heat Tolerance, Muhammad Ashraf, Fahad Al-Qurainy, Muhammad Sajid Aqeel Ahmad, Muhammad Yasir Iqbal, Adnan Mehmood, Alia Riffat, Ambreen Khadija Alvi

Turkish Journal of Botany

The aim of the present study was to examine the diversity for heat tolerance in a set of 105 accessions using some key morphophysiological attributes. The data was standardized by calculating percent change over control, and used for phonetic analysis and construction of dendograms based on distance matrix (all primary data is given as supplementary material listed in statistical analysis section of Materials and Methods). These distance matrices were then used for the unweighted pair group method with arithmetic mean (UPGMA) cluster analysis using the sequential agglomerative hierarchical nonoverlapping (SAHN) method. The cluster analysis indicated some potential attributes that could …

In Silico Analysis Of Xth Gene Family From Barley (Hordeum Vulgare L.) And Their Comparative Expression Analysis During Germination, Hülya Akdemi̇r Koç, Merve Seven, Ümi̇t Cem Derman, Andrew John Harvey

Turkish Journal of Botany

Changes in plant cell walls are critical for expansion and growth. Xyloglucan endotransglycosylase/hydrolase (XTH) enzymes are the major modifiers of xyloglucan within the cell wall and are present as large gene families. Despite the paucity of xyloglucan in barley, our analyses of the barley genome revealed at least 42 XTH genes, the most XTH members recorded for a monocot genome to date. In this paper, we show a detailed look at the barley XTH gene family, including detailed bioinformatics analyses of conserved protein motifs and structure, phylogenetic relationships, and a comparison of the expression patterns during germination and seedling growth …

Paraphlomis Nana (Lamiaceae), A New Species From Chongqing, China, Yaping Chen, Chi Xiong, Houlin Zhou, Feng Chen, Chunlei Xiang

Turkish Journal of Botany

Most species of Paraphlomis are distributed in the south of the Yangtze River in China. In this study, a new species, P. nana, from northeast Chongqing in the north of the Yangtze River is described and illustrated. Bayesian inference and maximum likelihood analyses based on two nuclear ribosomal DNA regions (ITS and ETS) and three plastid DNA markers (rpl32-trnL, rps16, and trnL-trnF) were carried out to explore the phylogenetic position of the new species within Paraphlomis. Though the nuclear and plastid trees reveal incongruent placements of P. nana in Paraphlomis, which may be caused by chloroplast capture, a close relationship …

New Species And New Record In Agaricus Subg. Minores From India, Entaj Tarafder, Arun Kumar Dutta, Krishnendu Acharya

Turkish Journal of Botany

Two new species of Agaricus, namely A. midnapurensis and A. purpureosquamulosus, belonging to A. subg. Minores, are proposed here as new to science based on specimens collected in West Bengal, India. Agaricus midnapurensis is distinguished by its medium-sized basidiomata with a pileus covered by greyish brown to brown or dark brown squamules, ellipsoid to broadly ellipsoid basidiospores with a mean value of 7.3 × 5.4 µm, and broadly clavate to pyriform cheilocystidia, 22-28 × 10-14 µm. Agaricus purpureosquamulosus has purplish coloured pileus squamules, ellipsoid to elongate basidiospores with a mean value of 7.3 × 4.5 µm, and clavate cheilocystidia measuring …

Iron Toxicity-Induced Dna Damage, Dna Methylation Changes, And Ltr Retrotransposon Polymorphisms In Zea Mays, Esra Arslan Yüksel, Murat Aydin, Mahmut Si̇nan Taşpinar, Güleray Ağar

Turkish Journal of Botany

The impact of Fe2+ (iron)toxicity on genomic instability, DNA methylation status, and Long Terminal Repeat Retrotransposons (LTR RTs) polymorphisms on Zea mays is unknown. We investigated the toxicity of Fe2+ using Random Amplified Polymorphic DNA (RAPD), Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) and Inter Retrotransposon Amplified Polymorphism (IRAP) assays in Zea mays seedlings, respectively. The results indicated that each dose of FeSO4 (50, 100, 200, and 300 mg/L) had a reducing effect on Genomic Template Stability (GTS) and increasing in RAPD pattern changes (DNA damage). The value of DNA methylation rised gradually depending on FeSO4 doses. Moreover, five LTR RTs …

Shackletonia Backorii - A New Species Of Lichenised Fungus From James Ross Island (Antarctic Peninsula), Mehmet Gökhan Halici, Mi̇that Güllü, Ekrem Bölükbaşi, Merve Kahraman Yi̇ği̇t

Turkish Journal of Botany

The new lichen species Shackletonia backorii Halıcı, Güllü & Kahraman is described from James Ross Island, which is located in the North-East Antarctic Peninsula region. The new species is morphologically most similar to S. insignis (Søchting & Øvstedal) Søchting, Frödén & Arup, which was described from Livingstone Island (North-West Antarctic Peninsula region). These two species have a similar bluish grey to blackish thallus with a microfruticose and coralloid structure and red brown or almost black zeorine apothecia, but S. backorii has rhomboid ascospores and grows on soil whereas S. insignis has ellipsoid ascospores and occurs in sheltered crevices of rocks. …

Genetic Diversity And Population Structure Analysis For Ampelopsis Grossedentata Using Microsatellite Markers, Xiao Ying Li, L Wei

Turkish Journal of Botany

Ampelopsis grossedentata is an important national medicine in China. It is mainly used commonly by the Yao, Miao, Dong, and other ethnic minorities of China. The current literature, however, contains no study on the molecular genetics and taxonomy of A. grossedentata, which had led to potential problems in the conservation and utilization of this resource. Therefore, the present study was aimed to use simple sequence repeat (SSR) markers for assessing the levels and the distribution of genetic diversity in a sample set of 225 individuals from nine populations of A. grossedentata in China. The genetic differentiation among the nine …

Genome-Wide Identification And Evolutionary Analysis Of Cngc Gene Families In Sixteen Brassicaceae Plant Genomes, Akram Ali Baloch, Kaleem U. Kakar, Asma Abro, Muhammad Mushtaq, Zarqa Nawaz, Abrar Hussain

Turkish Journal of Botany

Cyclic nucleotide-gated channels (CNGCs) are ligand-gated calcium signaling channels, play vital biological functions. Nonetheless, the CNGC gene families have not been well studied in Brassicaceae which includes commercially important and evolutionary model crops. Here, using in silico approaches, we recognized 414 CNGC genes in sixteen sequenced genomes of Brassicaceae species, which represent 10 genera of 8 tribes, ranging between 10-51 genes. These CNGCs can be classified into four main groups (I-IV) and two subgroups (IV-A and IV-B), of which, Group-II CNGC is the oldest that has gradually disappeared from the Arabis alpina L. genome. These results present a comprehensive overview …

Ecological Intensification With Soil Health Practices Demonstrates Positive Impacts On Multiple Soil Properties: A Large-Scale Farmer-Led Experiment, Fernanda Souza Krupek, Daren D. Redfearn, Kent M. Eskridge, Andrea Basche

Department of Agronomy and Horticulture: Faculty Publications

Improving soil health is critical to reversing trends of soil degradation and is of increasing interest to a range of stakeholders including policymakers, agricultural industry leaders, food companies, and farmers. Crop and soil management practices focused on ecological functions can be effective in restoring fundamental biological, chemical and physical soil properties. The call for ecological intensification of agricultural systems has the potential to improve soil health and input-use efficiency. In this study, we developed a framework to classify spatial and temporal ecological intensification with soil health practices: tillage, crop rotation, cover crop, organic amendment, and crop-livestock integration. We applied this …

Determinants Of Population Genetic Structure In Chamaecrista Fasciculata (Michx.) Greene (Fabaceae) In The Southeastern United States, Mahboubeh Hosseinalizadeh Nobarinezhad, Lisa E. Wallace

Biological Sciences Faculty Publications

Chamaecrista fasciculata is a widely distributed, phenotypically variable species in the eastern U.S. Whereas studies have demonstrated genetic structure and local adaptation in northern areas of its distribution, there has been no comparison of genetic variability among populations at the southern extent where phenotypic variation is more complex. We characterized genetic variation at 14 microsatellite loci for populations in Mississippi and Alabama and compared this to variation in a phenotypic trait, leaf pubescence. Geographic distance, climatic variables, and elevation were evaluated as factors to explain the observed patterns of genetic diversity. A significant amount of variation (19%) resided among populations, …

Influence Of Abiotic Drivers On 1-Year Seedling Survival Of Six Mangrove Species In Southeast Asia, Taylor M. Sloey, Kiah Eng Lim, Jared Moore, Jie Min Heng, Jia Min Heng, Michiel Van Breugel

Biological Sciences Faculty Publications

Establishment and survival of plant species in systems with dominant environmental drivers (i.e. factors that exert disproportionate control over species establishment and survival) is often thought to be dominated by one master variable. In forested wetlands such as mangroves, hydrology is typically considered the dominant limiting driver. At the same time, light is a major driver of plant community dynamics, with some of the best understood plant life-history tradeoffs related to fast growth under high-light conditions versus survival under low-light conditions. Yet light is given relatively limited consideration in mangrove research compared to other drivers. Understanding the relative importance of …

A Flora Of Coyote Ridge And Flat, Inyo County, California, Martin Purdy

CGU Theses & Dissertations

California’s Sierra Nevada mountain range contains a disproportionate share of the state’s plant diversity and is one of the most floristically diverse regions of its size in the United States. The high Sierra Nevada, in particular, has been identified as an important center of species richness and endemism within California. Anthropogenic climate warming is expected to disproportionately affect mountain ecosystems, and models have predicted serious habitat contraction and extirpation for many alpine plant taxa. A specimen-based inventory of the vascular and non-vascular plants of Coyote Ridge and Flat was conducted to establish baseline data for one such sensitive alpine and …

The Challenges Of Growing Orchids From Seeds For Conservation: An Assessment Of Asymbiotic Techniques, Devani Jolman, Martín I. Batalla, Alexis Hungerford, Pryce Norwood, Noah Tait, Lisa E. Wallace

Biological Sciences Faculty Publications

Lewis Knudson first successfully germinated orchid seeds asymbiotically on artificial medium in 1922. While many orchid species have since been grown asymbiotically, the tremendous variation in how species respond to artificial medium and growth conditions ex situ has also become apparent in the past century. In this study, we reviewed published journal articles on asymbiotic orchid seed germination to provide a summary of techniques used and to evaluate if these differ between terrestrial and epiphytic species, to identify areas where additional research is needed, and to evaluate whether asymbiotic germination could be used more often in ex situ conservation. We …

Identification And Characterization Of A Long Fruit Mutant In Cucumis Sativus L., Yajing Hu, Xiaojing Dong, Yana Zhang, Rui Tang, Jiali Li, Chenxing Cao, Chunhua Chen, Zhonghai Ren, Lina Wang

Turkish Journal of Botany

A long fruit (lf) mutant was obtained from the cucumber inbred line 'SN5' by ethyl methanesulfonate (EMS)-mediated mutagenesis. We found that plant height and internode length of lf were significantly decreased, while leaf area was significantly increased in comparison to wild type (WT) plants. Higher carbohydrate level, together with lower chlorophyll content and photosynthetic parameters, was revealed in lf leaves than those of WT. Longer fruits, as well as enhanced fruit quality, were observed for mutant plants in comparison to WT, and the difference in fruit length was continuously increased with the development of fruits. ELISA and real-time PCR analyses …

Arundinella Stenostachya (Arundinella, Poaceae), A Reevaluated New Species From Se Asia, Liqiong Jiang, Zhenwen Liu, Ende Liu, Atchara Teerawatananon, Yuehua Wang, Hua Peng

Turkish Journal of Botany

Arundinella stenostachya, a new species, is described and illustrated. It is morphologically similar to A. decempedalis and A. bengalensis, but differs from the former species by the length of panicle branches, type of trichome on nodes and pedicels length, and from the latter in height and diameter of culms, the length and width of leaf blades, pedicels length, and the length of panicles and branches. The new species has been mistakenly identified as A. decempedalis and A. bengalensis. Molecular analyses based on ITS sequences also support A. stenostachya as a distinct species.

Dna Barcoding Analysis And Phylogenetic Relationships Of Indian Wild Coffee Species, Manoj Mishra, Pavankumar Jingade, Arun Kumar C Huded

Turkish Journal of Botany

Wild coffee species are the reservoirs of genetic diversity and could play a critical role in the genetic improvement of coffee. However, the conservation and genetic assessment of wild coffee species has been largely neglected. In the present study, DNA barcoding approaches were employed to assess the phylogenetic relationships between five indigenous wild coffee species from India and compared with two cultivated, and four wild coffee species of African origin. The efficacy of three barcoding loci namely matK,rbcL, and trnL-trnF was investigated using PCR amplification and sequence characterization. The intergenic spacer trnL-trnF is the highly polymorphic loci followed by matK …

Chromosome Numbers And Genome Size Data On Species Of The Genus Petrorhagia(Caryophyllaceae) From Turkey, Kamuran Aktaş, Jaume Pellicer, Teresa Garnatje

Turkish Journal of Botany

Genome size, i.e. the total amount of DNA in an unreplicated somatic nucleus is considered an important character in the biology of organisms, and it is especially relevant for land plants given the extraordinary diversity reported. Despite the continuous growth of data and the efforts aimed at increasing our understanding of plant genome size diversity, more data are needed to have an evenly representation across lineages. With this premise in mind, in this work, we investigated genome size, complemented with chromosome data in eight species of the genus Petrorhagia (Caryophyllaceae) from Turkey. Chromosome counts made indicate that the species studied …

Current Nomenclature And Systematics Of Capsella Medik. With Lectotypifications:Towards Solving The Puzzle, Yelda Güzel

Turkish Journal of Botany

The taxonomic history of Capsella Medik., one of the genera with the highest phenotypic plasticity, and thus the highest synonym number relative to the recognized species number, was reviewed. The current systematics of the genus was discussed and the exact number of taxa was determined. In light of the molecular and nomenclatural data, it was decided that the genus was represented around the world by five species and one hybrid. Considering the molecular studies that have been conducted over the 30 years it was concluded that the species of this genus are typical examples of the concept of cryptic species. …

Tripleurospermum Eskilensis (Asteraceae): A New Halophytic Species From Centralanatolia, Turkey, Mehtap Tekşen, Seher Karaman Erkul, Hayri̇ Duman, Mevlüde Alev Ateş, Mehmet Sağiroğlu

Turkish Journal of Botany

In the present study, a new species of the genus, Tripleurospermum eskilensis (Asteraceae), is described from Central Anatolia, Turkey. The new species has been collected from Eskil region in a restricted area (Aksaray Province). It grows in salty steppe, and is closely related to T. decipiens in terms of morphological characteristics. It has also been morphologically compared with discoid and disciform capitulated species in Turkey. This study includes its detailed description, diagnostic characters, original photographs, geographical distribution, habitat and ecology, conservation status, and identification key. The comparison of Tripleurospermum eskilensis with closely related species was conducted in terms of morphological …

Effect Of Seaweed-Based Liquid Extracts On Biomass Production And Lipid Accumulation In Nannochloropsis Oculata And Dunaliella Salina, Krishna Moorthy Abarna, Rani Velu, Padmavathy P, Jawahar P, Uma A, Kalidas C, Satya Prakash Shukla

Turkish Journal of Botany

Seaweed liquid fertilizers (SLFs) contain macronutrients, micronutrients and trace nutrients essential for microalgal growth. The study aims to evaluate the efficiency of SLFs on the biomass production, biochemical and physiological processes; and the study was carried out at different SLF concentrations of selected seaweeds i.e. Kappaphycus alvarezii, Gelidiella acerosa and Turbinaria ornata. The comparative analysis of the growth performance of two unicellular green alga on three different organic media suggest that the liquid fertilizer from red seaweed was highly efficient than the SLF prepared from brown seaweed. The growth rate of N. oculata and D. salina in K. alvarezii SLF …

Archaeobotanical Evidence Of Agricultural Products In The Southern Mediterranean Part Of Turkey During The Bronze Ages From Tatarlı Höyük, Sali̇h Kavak, Hali̇l Çakan

Turkish Journal of Botany

In this study, archaeobotanical data were gathered from the Early, Middle, and Late Bronze Age layers (2400-1200 BC) of the Tatarlı Höyük exposed in the 2011?2012 excavation seasons in the Ceyhan district of Adana Province were evaluated. In 8 trenches relating to these periods, archaeological contexts such as inside areas, hearths, ovens, middens, ceramic pots, etc., as well as contexts with carbonized plant remains were recovered by using dry and wet sieving methods, corresponding in total to 90 soil samples. The major agricultural plants identified were Triticum monococcum (einkorn wheat), Triticum dicoccum (emmer wheat), Triticum aestivum (bread wheat), Hordeum vulgare …

Chemical Fingerprints And Bioactivities Of 12 Anatolian Achillea Species By Lc-Ms/Ms With Chemometric Approach: Novel Phytonutrients, Natural Food Preservatives And Chlorogenic Acid Sources, Mustafa Abdullah Yilmaz, Abdulselam Ertaş, İsmai̇l Yener, Fatma Pinar Türkmenoğlu, Özge Tokul Ölmez, Mehmet Öztürk, Muhammed Altun, Oğuz Çakir, Abbas Tarhan, Mehmet Boğa, İbrahi̇m Demi̇rtaş, Mehmet Hakki Alma, Hamdi̇ Temel

Turkish Journal of Botany

The objective of this study was to evaluate the biological activities and chemical fingerprint profiles of the extracts obtained from twelve Achillea L. species (A. lycaonica, A. biebersteinii (syn: A. arabica), A. kotschyi subsp. kotschyi, A. schischkinii, A. millefolium subsp. millefolium, A. sintenisii, A. setacea, A. teretifolia, A. wilhelmsii subsp. wilhelmsii (syn: A. santolinoides subsp. wilhelmsii), A. nobilis, A. goniocephala, A. spinulifolia). The antioxidant, enzyme inhibitory and cytotoxic effects were evaluated to investigate their bioactivity profiles. Furthermore, the total flavonoid and phenolic contents were determined and LC-MS/MS analysis was performed to reveal the phytochemical profile of the investigated extracts. A. …

Taxonomy And Phylogeny Of A New Species Of Pseudocercospora On Solanum Nigrum From India, Archana Singh, Sanjay Yadav, Raghvendra Singh, Nawal Dubey

Turkish Journal of Botany

A new species Pseudocercospora solanicola is discovered causing severe leaf spot disease on Solanum nigrum from Sonebhadra. Species is described and illustrated based on morphology, molecular sequence analysis and phylogeny of the large subunit (LSU) and internal transcribed spacer region of the nuclear ribosomal RNA (rRNA) gene. Morphologically, new species has similarity with allied species in Pseudocercospora but differs in having black circular, velvety leaf spot and size of stromata, conidia and conidiophores. A key to Pseudocercospora species on Solanum has been provided.

Lamium Cappadocicum, A New Species From Central Anatolia, Turkey: Evidence From Molecular And Morphological Studies, Ferhat Celep, Fergan Karaer, Bryan T. Drew

Turkish Journal of Botany

Lamium is a taxonomically convoluted genus of about 34 species. Within Lamium, the L. garganicum species complex is particularly challenging. Here, based on morphological and molecular studies, Lamium cappadocicum Celep & Karaer sp. nova (Lamiaceae) is separated from L. garganicum and L. bilgilii and described as a new species, and L. garganicum subsp. rectum (= L. garganicum subsp. pulchrum) is resurrected. The new species is only known from the Hasan Mountain (Aksaray) in Central Anatolia, Turkey. Lamium cappadocicum is closely related to L. garganicum subsp. rectum but differs from it by its mat-forming caespitose habit, reniform (rarely ovate …

Morphological And Molecular Characterization Of Banana Clones Growing In Turkey, Fi̇li̇z Baysal, Sezai̇ Erci̇sli̇

Turkish Journal of Botany

This study was conducted to estimate genetic relationships among banana clones growing in Turkey via some morphological parameters and two molecular marker systems. In terms of yield parameters such as bunch weight, hand number, fruit weight, and total fruit number, Grand Nain (GN) clone came to the forefront with the highest values. It was followed by Azman (AZ), Dwarf Cavendish (DC), and Erdemli Yerli (EY) clones, respectively. To see the variation between clones more clearly, 24 RAPD (Random Amplified Polymorphic DNA) primers and 48 SRAP (Sequence Related Amplified Polymorphism) primer combinations were used in molecular analysis. The total number of …