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Organogenesis Of Ammi Majus Via Callus Culture From Leaf., Sandip S. Magdum, Sanjeev Kumar

Sandip S. Magdum

Ammi majus L. is an imperiled medicinal plant, which contains various medically important and uncommon extraordinary secondary metabolite compounds. An efficient organogenesis protocol was established for Ammi majus through in vitro process of leaf callusing, shooting and rooting by using different combination of auxins and cytokinins. In vitro cultured juvenile leaf explants were used directly for callus formation on MS supplemented with different concentration of IAA, Kn and CH. Maximum percentage response for callus formation was 97.7 after 5 weeks of incubation on MS supplemented with 2 mg/l IAA, 2 mg/l Kn and 1000 mg/l CH. About 81% of calluses …