Agronomy and Crop Sciences Commons^™

Transgenesis And Genomics In Molecular Breeding Of Forage Plants, G. Spangenberg, R. Kalla, A. Lidgett, T. Sawbridge, E. K. Ong, U. John

IGC Proceedings (1977-2023)

Forage plant breeding has been largely based on phenotypic selection following sexual recombination of natural genetic variation found between and within ecotypes. Advances in plant genetic manipulation over the last 15 years have provided convincing evidence that these powerful technologies can complement and enhance plant breeding programs. Significant progress in the establishment of the methodologies required for the molecular breeding of forage plants has been made. Examples of current products and approaches for the application of these methodologies to forage grass and legume improvement are outlined. Large-scale genomic analysis of many organisms is under way with human, arabidopsis and rice …

Opportunities In Molecular Biology: Enhancement Of The Nutritional Value Of Forages, K. R. Hancock, M. J. Ulyatt

IGC Proceedings (1977-2023)

Pasture plants, while producing a cost-effective source of feed for grazing ruminants, can frequently be less than optimal in meeting the animals’ nutrient requirements. Over the past half century, there has been a major effort to improve the quantity and nutritional quality of pasture plants using conventional plant breeding. Although considerable progress has been made in improving the quality and agronomic characteristics of our major pasture plants by this means, breeding can only be applied to plants capable of sexual crosses. This poses severe limitations both in terms of speed of progress and in the number of genes available for …

The Role Of Endophytic Fungi In Brachiaria, A Tropical Forage Grass, S. Kelemu, J. F. White, Idupulapati M. Rao

IGC Proceedings (1977-2023)

In temperate zones, endophytic fungi are widely used as biological protection agents for forage and turf grasses. They form nonpathogenic and intercellular associations with grasses and sedges, completing their entire life cycle within the plants’ aerial parts. Our surveys and studies confirmed that various endophytic fungi, including Acremonium spp., also inhabit native savanna grasses and introduced tropical forage grasses. We are now determining the potentially symbiotic relationships between these fungi and C₄ tropical forages, specifically between the endophyte A. implicatum and Brachiaria grasses. We treated half of a group of genetically identical clones of Brachiaria with fungicide to generate …

Advances In Endophyte Research. Progress And Priorities In Temperate Areas, L. R. Fletcher, H. S. Easton

IGC Proceedings (1977-2023)

Fungal endophytes infect a large number of temperate grass species, genera and tribes (Leuchtmann and Clay, 1997). The majority of these systemic endophytes fall into the genus Epichloe or the closely related Neotyphodium genus.

The asexual Neotyphodium endophytes are asymptomatic, never emerge from between the host grass cells, and are only transmitted vertically, via seed of the host plant. They are believed to have derived from the Epichloe endophytes, (Bacon and White, 2000; Schardl and Wilkinson, 2000) which do emerge from their intercellular habitat and form stromata around the emerging seedhead of their host. This is manifested as the choke …

Advances In Rhizobial Research – Progress Priorities In Temperate Areas, Angela Sessitsch

IGC Proceedings (1977-2023)

Rhizobia are well known for their capacity to establish a symbiosis with legumes. During this symbiosis the bacteria inhabit root nodules where they reduce atmospheric nitrogen and make it available to the plant. Biological nitrogen fixation (BNF) is an important source of nitrogen and the various legume crops and pasture species often fix as much as 200-300 kg nitrogen per hectare (Peoples et al., 1995). Globally, symbiotic nitrogen fixation has been estimated to amount to at least 70 million metric tons of nitrogen per year (Brockwell et al., 1995). Furthermore, in many cases nitrogen fertilizers are not efficiently used by …

The Legume – Rhizobia Symbiosis. Does It Vary For The Tropics Relative To The Mediterranean Basin?, J. G. Howieson, A. Mcinnes

IGC Proceedings (1977-2023)

Symbiotic N fixation from legumes is one of the most important biological processes on the planet. It currently provides the majority of the N requirement in agriculture, yet will have to double if cereal crop production is to meet world demand by 2020 (Kennedy and Cocking 1997). To effectively harness the value of biological N fixation from legumes we need to more fully understand G² x E; where G refers to the genotypes of both the legume (G_l) and its microsymbiont (rhizobia; G_r), and E refers to the edaphic environment in which the symbiosis is …

Advances In Forage Legumes: Shrub Legumes, M. H. Shelton

IGC Proceedings (1977-2023)

Forage tree legumes benefit agriculture through their multi-purpose contributions to livestock feeding, to productivity of farming systems, and to protection of the environment.

Of the several hundred forage tree legume species regarded as having potential for forage only c. twenty are in common use. These are listed together with their agroecological range and tolerance of adverse environments including acid soils, cool temperatures, low rainfall, poor drainage and high salinity. The value of many species to ruminants is limited due to low nutritive value arising from low protein availability and low acceptability caused principally by high condensed tannin content. Only 10 …

Alfalfa, J. H. Bouton

IGC Proceedings (1977-2023)

Cultivated alfalfa or lucerne (Medicago sativa spp. sativa) is the most important forage legume in the world with approximately 32 million hectares cultivated mainly in the temperate regions of both the northern and southern hemispheres. Intensive research has been conducted on the management and physiology of the crop and a major seed industry has developed world wide. Since insect and disease pests are numerous in alfalfa, development of cultivars with the proper fall dormancy and a broad genetic base for pest resistance has been pursued in order to provide increased adaptation, persistence, and yield. There has been a …

Carbon And Nitrogen Isotopic Discrimination In Alfalfa For High Yield And Quality Forage, W. S. Oliveira, P. P. A. Oliveira, M. Corsi, S. M. Tsai

IGC Proceedings (1977-2023)

Biological nitrogen fixation and assimilation of CO₂ can be indirectly monitored in alfalfa (Medicago sativa L.) through the variation of the stable N and C isotopes in the plant (Δ values). Such measurements result in a simple, non-destructive and reliable method for identification of alfalfa cultivars of high yield and nutritional quality. A program for selection of 12 alfalfa cultivars under field and controlled conditions (lysimeters) included determination of biomass and N accumulation. Under controlled conditions yields ranged from 1.77 to 2.47 ton/ha/month (Pioneer 5312 and WL-605) and N2-fixed varied from 77.43 to 84.01% for total N (Brazilian …

Stolon Growth And Its Morphological Components In White Clover (Trifolium Repens L.) Cultivars, Omar Scheneiter, E. M. Pagano

IGC Proceedings (1977-2023)

A trial was performed to study the seasonal changes in stolon growth and its morphological components in four white clover cultivars, in the north of Buenos Aires Province, in Argentina. Stolons were tagged in four seasonal measurements periods per year, during two consecutive years. Leaf, stolon and flowerheads weights were recorded at regular intervals in each measurement period and total stolon growth was calculated. Differences among cultivars were found in stolon growth and its morphological components in most of the measurement periods. In spring and summer, differences among cultivars in stolon growth were related to flowering quantity and earliness, and …

Dry Matter Production Of Shoots And Root Density Of Two Cultivars Of Lablab Purpureus (L.) Sweet, L. R. De A. Rodrigues, T. J. D. Rodrigues, A. K. B. Ramos, D. G. De Quadros

IGC Proceedings (1977-2023)

This experiment was conducted in green house conditions to evaluate the DM accumulation in the shoots and in the roots of two cultivars of Lablab purpureus (L.) Sweet. A 2x3 factorial (two cultivars and three evaluation dates) was conducted according to a randomized complete block design with four replications, being the cultivars Highworth and Rongai evaluated at 42, 56, and 70 days after seedling emergence (DASE). The results indicated that the cvs. Highworth and Rongai have the same pattern of DM accumulation in the shoots. In the upper layer of the soil (0-0.20 m) it was found 38.83% and 43.64% …

Yellow-Flowered Lucerne (Medicago Falcata L.) - A Potential Forage Legume For The North, T. Mela, H. Ihamäki, M. Matilainen

IGC Proceedings (1977-2023)

The suitability of yellow-flowered lucerne (Medicago falcata L.) for forage production under the northern conditions was studied in a mixture sward with timothy (Phleum pratense L.) in field trials in South Finland for four years from 1995 to 1998. Through the first three winters lucerne stayed unharmed. In June and July lucerne grew vigorously, but in August little. The total seasonal yields were highest in the second and the third year of harvesting, up to 10 t DM ha^-1. In the winter 1997-1998 , winter damages killed 21 to 38 % of lucerne plants, and resulted …

Role Of White Clover In The Improvement Of Economical Efficiency Of Feeds From Grasslands, P. Goliñski

IGC Proceedings (1977-2023)

The aim of the research was to analyse the role played by white clover in the improvement of economical efficiency of feeds obtained from permanent grasslands. The influence of the proportion of white clover in the pasture sward on unit production costs of yield, energy and crude protein and the profit threshold in milk production was analysed taking into account three white clover cultivars, nitrogen fertilisation, irrigation and overdrilling. The investigation procedures employed the author’s own calculation models elaborated in optimisation programs based on production conditions found in dairy farms in the region of Wielkopolska. It was proved that the …

The Use Of Cafeteria Trials For The Selection Of Desmodium Ovalifolium Genotypes, Axel Schmidt, R. Schultze-Kraft, B. L. Maass, C. E. Lascano

IGC Proceedings (1977-2023)

For the selection of tropical legumes which contain anti-nutritive components such as tannins, relative acceptability of genotypes to ruminants is of particular importance, since these plant components may influence selective grazing behaviour and subsequent animal productivity. Plant-animal interactions are not predictable from laboratory analyses. Involving grazing animals through the conduction of relative-acceptability (=cafeteria) trials at early stages of the germplasm selection process might therefore provide a convenient tool to adjust and confirm genotype selection based on laboratory quality analyses data. As part of a multilocational germplasm evaluation project, cafeteria-experiments were conducted at two contrasting environments in Colombia with a core …

Stolon Mass And Classes In White Clover (Trifolium Repens L.) With Two Soil Water Availability Levels, Omar Scheneiter, E. M. Pagano

IGC Proceedings (1977-2023)

Two trials were carried out to study the stolon mass and classes in white clover (Trifolium repens L.) under two soil water availability levels in summer. In each trial, treatments were two white clover cultivars. From December 15^th to February 15^th in 1993/94 and again during the same period the following year, white clover was grown under 30 to 70 % (Forced water uptake) or 85-to100 % (Maximum water uptake) of field capacity. Every two months, throughout two years, total stolon mass, and buried, superficial, and aerial stolons were measured. Differences between cultivars were detected in stolon …

Morphological Description Of Selected Pigeonpea (Cajanus Cajan (L.) Millsp.) Lines, Rodolfo Godoy, F. H. De Souza

IGC Proceedings (1977-2023)

Seventeen pigeonpea pure line accessions selected for desirable forage traits and three commercial cultivars were described according to morphological characters. The following descriptors were used: growth habit, plant height, number of primary and secondary branches, stem color and thickness, leaf shape and hairiness, flower base and second color, pattern of flower streaks, flowering pattern, immature and mature pod color, pod form and hairiness, seed color pattern, base and second seed color, seed eye (region around hilum) color and seed shape and width. Each individual line and cultivar was satisfactorily distinguished from the others by the chosen characters.

Improvement Of Rose Clover Winter Forage Production, Gerald R. Smith, F. M. Rouquette Jr.

IGC Proceedings (1977-2023)

Rose clover (Trifolium hirtum All.) is a winter annual forage legume that has potential for increased use in the U.S. southern region. New cultivars of rose clover are needed with the combination of cold tolerance, winter forage production and full bloom date of late April. Crosses were made between two early flowering cultivars and one late flowering rose clover cultivar. The F₂ and F₃ generations from these crosses were evaluated in northeast Texas for winter growth, cold damage and date of flowering. Fourteen F₃ lines, out of 37 selected F2 parents, showed large increases (>100%) …

Evaluation Of Brachiaria Brizantha Ecotypes Under Grazing In Small Plots, Valéria P. B. Euclides, Cacilda B. Do Valle, Manuel C. M. Macedo, M. P. Oliveira

IGC Proceedings (1977-2023)

An intense search for new cultivars amongst recently collected and introduced ecotypes from Africa is in effect since 1988, at the National Beef Cattle Research Center, of the Brazilian Agricultural Research Corporation (Embrapa Beef Cattle). After agronomic evaluations of a large collection, 21 ecotypes were pre-selected for multilocational trials and from these, eight were elected for evaluation under grazing. The objective of this trial was thus to evaluate the persistence and carrying capacity of these eight new Brachiaria brizantha ecotypes after two years of grazing. The experimental design was a randomized complete block with nine treatments and two replicates. The …

In Vitro Propagation Of Pennisetum Purpureum Schum., M. M. G. Karasawa, J. E. B. P. Pinto, A. V. Pereira, José C. Pinto, F. G. Silva

IGC Proceedings (1977-2023)

A protocol is described for rapid multiplication of elephantgrass (Pennisetum purpureum Schum.) through shoot tip culture. The plant growth medium consisted of basal medium of Murashige and Skoog (MS) and vitamins Wood Plant Medium (WPM). The medium was supplemented with 0.00; 4.44; 8.88; 13.32 and 17.76 μM of benzylaminopurine (BAP). The elephantgrass was micropropagated by axillary shoot proliferations. Maximum propagule proliferation occurred on Murashige and Skoog (MS) medium enriched with 4.4 μM benzylaminopurine (BAP), resulting an average of 4.0 shoots per explant from cultivar Mineiro and 2.19 from cultivar Pioneiro. The best height and root plantlets were obtained with …

'Stay-Green' And Non-'Stay-Green' Perennial Ryegrass In Field Swards With Different Intervals Between Cuts, D. Wilman, K. R. Kennedy, M. O. Humphreys

IGC Proceedings (1977-2023)

The purpose of this study was to investigate the effect of the 'stay-green' character, originally introduced into perennial ryegrass (Lolium perenne L.) for amenity purposes, on herbage yield, N concentration and colour in field swards of 'stay-green' forage ryegrass managed in different ways for agricultural use. The conclusions were that the introduction of the 'stay-green' character (1) confers greater greenness only at some times of year and only when there has been a sufficiently long period of regrowth, (2) is expressed only in older, as distinct from young, tillers, (3) reduces herbage yield, particularly when a long interval is …

Agronomic Evaluation Of Alfalfa Cultivars In Rio Grande Do Sul, Brasil, J. C. De Saibro, R. Battisti, T. M. S. Freitas

IGC Proceedings (1977-2023)

From April 1995 to June 1998, thirty five alfalfa (Medicago sativa L.) cultivars (cvs.) were evaluated in a field trial at the “Depressão Central” ecoclimatic region, in southern Brazil. Two years after sowing, 30 cultivars were excluded from the test due to their poor overall agronomic performance, mainly low forage DM yields. The remaining cultivars: Crioula (local genotype used as a check), Victoria, Rio and P 30 are hay-type cultivars while Alfagraze is a grazing-tolerant type. Twenty-two harvests were made and no significant differences were found for total DM yields among cvs. Crioula, Victoria, Rio and P 30; however, …

Switchgrass For Biomass Production, Monroe Rasnake, D. Parrish, Garry D. Lacefield, D. Wolf

IGC Proceedings (1977-2023)

Six cultivars of switchgrass (Panicum virgatum) were established in 1993 at Princeton, Kentucky, USA. Two harvest systems were used: two cuttings per year in mid-June and November and one cutting per year in November. Biomass yield and stand survival were evaluated. Lowland cultivars produced more biomass than upland cultivars in both cutting managements. Upland cultivars did better in a two cut system while the other cultivars showed little difference. Excellent stands of all cultivars were maintained under both cutting managements.

Metabolic Engineering Of Peanut Hairy Roots Via Rna Interference To Study The Role Of Prenylated Stilbenoids Against Water Stress, Gaurav Gajurel

Student Theses and Dissertations

Abiotic stress, such as drought, causes over five-hundred million dollars in losses in peanut production per year in the world. Peanuts are known to produce antioxidant prenylated stilbenoids, however, their role in abiotic stress tolerance have not been elucidated. The overall goal of this project was to determine if selected prenylated stilbenoids play a role against water stress in peanut. To address this issue, transgenic hairy roots with the capacity to produce diverse prenylated stilbenoids using an RNA-interference (RNAi) approach were established. RNAi molecular constructs targeting a stilbenoid-specific prenyltransferase were designed, synthesized, and expressed via Agrobacterium rhizogenes-mediated transformation in peanut …

Genome-Wide Dna Polymorphism Analysis And Molecular Marker Development For The Setaria Italica Variety “Ssr41” And Positional Cloning Of The Setaria White Leaf Sheath Gene Siwls1, Hui Zhang, Sha Tang, James C. Schnable, Qiang He, Yuanzhu Gao, Mingzhao Luo, Guanqing Jia, Baili Feng, Hui Zhi, Xianmin Diao

Department of Agronomy and Horticulture: Faculty Publications

Genome-wide DNA polymorphism analysis and molecular marker development are important for forward genetics research and DNA marker-assisted breeding. As an ideal model system for Panicoideae grasses and an important minor crop in East Asia, foxtail millet (Setaria italica) has a high-quality reference genome as well as large mutant libraries based on the “Yugu1” variety. However, there is still a lack of genetic and mutation mapping tools available for forward genetics research on S. italica. Here, we screened another S. italica genotype, “SSR41”, which is morphologically similar to, and readily cross-pollinates with, “Yugu1”. High-throughput resequencing of “SSR41” …

Evaluation Of Paspalum Spp. With Adaptation To Poorly Drained Soils In The Tropical Americas, Michael Peters, B. Hincapié, P. Avila, C. E. Lascano

IGC Proceedings (1977-2023)

A collection of Paspalum accessions obtained from Brazil was tested for their adaptation to poorly drained soils. Three accessions were selected based on their DM production and nutritive value. Productivity and quality of these three accessions was stable over the periods of maximum and minimum rainfall, giving potential for continuous forage supply throughout the year in the tropical Americas. Grazing trials for the selected accessions are underway.

Intake Characteristics And Performance Of Contrasting Grass Varieties Continuously Stocked With Sheep, R. J. Orr, J. E. Cook, R. A. Champion, A. J. Rook

IGC Proceedings (1977-2023)

Fifteen intermediate-heading perennial ryegrass (Lolium perenne) varieties were grazed by sheep under continuous variable stocking management. Mean daily grass dry matter intake per ewe was positively correlated with liveweight gain per ewe (r = 0.63) and per ha (r = 0.58) over an 8-month grazing season. Daily intake was not correlated with grazing time (r = 0.12) but was positively correlated with instantaneous dry matter intake rate (r = 0.75). Breeding varieties with high intake rate characteristics should allow increases in the contribution of grazed grass to grassland-based livestock farming. There is now a …

Drought Tolerance Of Interspecific Hybrids Between Trifolium Repens And Trifolium Ambiguum, A. H. Marshall, M. T. Abberton, T. P. T. Michaelson-Yeates, I. Rhodes, A. Williams

IGC Proceedings (1977-2023)

Hybrids between the stolonifeous white clover (Trifolium repens L., 2n=4x=32) and rhizomatous Caucasian clover (T. ambiguum M.Bieb, 2n=4x=32) have been produced. A backcross 2 (BC2) generation with white clover as the recurrent parent combines the growth habit of both parent species with the objective of increasing the persistency of large leaved T. repens varieties under grazing. T. ambiguum is more drought tolerant than T. repens. The drought tolerance of the hybrids in comparison with the parental species was compared in deep soil bins over a four week drought cycle. Soil moisture content, leaf relative water content …

Variability For Nitrogen And Phosphorus Uptake Among Timothy Genotypes, Réal Michaud, Gilles Bélanger, A. Brégard

IGC Proceedings (1977-2023)

Cultivars of timothy (Phleum pratense L.) with high N and P use efficiencies are required to reduce costs of production and risks of N and P pollution, and to meet the nutrition requirements of high producing animals. This field study aimed at comparing, (under three N rates) the relationship between DM yield, N uptake, and P uptake of 27 timothy half-sib families, plus the cultivar Champ as a control (Dry matter yield), N and P uptake increased with increasing N rates. Genotypes also differed for DM yield, N and P uptake. For a given DM yield, the genotypes had …

New Stylosanthes Guianensis For Tropical Grasslands, Bela Grof, Celso D. Fernandes, A. T. F. Fernandes

IGC Proceedings (1977-2023)

Hybrid stylos (Stylosanthes guianensis var. vulgaris x var. pauciflora) with durable, quantitative resistance to anthracnose, mid-season harvest maturity date (early-July), high DM and seed yields have been selected at the Embrapa Beef Cattle Research Center, Campo Grande, Brazil. The hybrids displayed improved forage traits in Brazil, the native habitat and major center of diversity of the species and its pathogen, as well as in vastly different ecosystems. Dry forage yields and anthracnose resistance of superior selections and their composites were equal, in some instances, significantly better, than those of cv. Mineirão in multilocational trials situated in the Cerrados from …

A Chickpea Genetic Variation Map Based On The Sequencing Of 3,366 Genomes, Rajeev K. Varshney, Manish Roorkiwal, Shuai Sun, Prasad Bajaj, Annapurna Chitikineni, Mahendar Thudi, Narendra P. Singh, Xiao Du, Hari D. Upadhyaya, Diego Jarquin

Department of Agronomy and Horticulture: Faculty Publications

Zero hunger and good health could be realized by 2030 through effective conservation, characterization and utilization of germplasm resources1 . So far, few chickpea (Cicerarietinum) germplasm accessions have been characterized at the genome sequence level2 . Here we present a detailed map of variation in 3,171 cultivated and 195 wild accessions to provide publicly available resources for chickpea genomics research and breeding. We constructed a chickpea pan-genome to describe genomic diversity across cultivated chickpea and its wild progenitor accessions. A divergence tree using genes present in around 80% of individuals in one species allowed us to estimate the divergence of …