Agronomy and Crop Sciences Commons^™

Quantitative Imaging Of Cell-Level Auxin Cytokinin Outputs In Soybean And Model Plants, Paul Gaillard

Electronic Theses and Dissertations

Auxin and cytokinin are major plant hormones that orchestrate plant development. These hormones are involved in cell division and differentiation and their spatio‐temporal balance controls organ initiation and maturation. Even though independent transcriptional responses to these hormones have been characterized, their concomitant outputs have not been described. We developed methodology to determine auxin-cytokinin relative output (ACRO) ratios at cellular resolution. Hormone-specific fluorescent markers that localize to the nucleus were used to report transcriptional outputs of auxin and cytokinin. Multiphoton microscopy and image data processing were used to quantify their relative ratio at cellular level in three-dimensional tissue volumes. This strategy …

Use Of Unmanned Aerial System (Uas) For High Throughput Evaluation Of Forage Yield In Oat Breeding Nurseries, Prakriti Sharma

Electronic Theses and Dissertations

Current strategies for phenotyping (for traits like biomass) numerous breeding lines under field conditions demand significant investment in both time and labor. Unmanned aerial systems (UAS) can be used to collect vegetation indexes (VI) with high throughput and could provide an efficient way to predict forage yield in breeding nurseries with accuracy. The main objective of the study was to evaluate the use of VIs derived from UAV collected images for estimating crop biomass. For this study, forage trials consisting of 35 oat genotypes were carried out at three locations in 2018 and four locations in 2019. Unmanned aerial vehicles …

Letter From The Faculty And Managing Editors, Beth Kegley, Gail Halleck

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Estimation Of Additive And Dominance Effects Of A Mutant Glutathione S-Transferase Gene On Anthocyanin Content In Muscadine Grape (Vitis Rotundifolia), Autumn Brown, Margaret Worthington, Aruna Varanasi, Lacy Nelson, Renee T. Threlfall, Luke R. Howard

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The skin color of muscadine grapes (Vitis rotundifolia) is typically classified as black or bronze. A glutathione S-transferase, VrunGST4, has been identified as a candidate gene for berry skin color in muscadine grapes. A molecular marker was developed within VrunGST4 to distinguish between muscadine genotypes (cultivars and selections) with bronze (T:T), heterozygote black (C:T), and homozygote black (C:C) berries. The objectives of this study were to determine whether there was a correlation between berry skin color and total anthocyanin content and to calculate additive and dominance effects of VrunGST4 in determining total anthocyanins in the berries of two biparental F1 …

Food Accessibility Related To The Double Your Dollar Program, Julia Carlson, Heather Friedrich, Mechelle Bailey, Curt Rom

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The Double Your Dollar (DYD) Program is a program that gives Supplemental Nutrition Assistance Program (SNAP) beneficiaries and Senior Farmers Market Nutrition Program (SFMNP) participants match dollars to spend at local farmers markets. The DYD’s goal is to incentivize healthy eating among individuals of low income and promote spending at farmers markets. Food insecurity affects over 60,000 individuals in Washington and Benton counties in Arkansas. The aim of this study was to assess how the DYD program impacted users’ food accessibility and how the program could be improved for the future. A survey was used to address basic demographics, type, …

The Biofiltration Ability Of Asparagus Densiflorus To Remove Sulfur Dioxide From The Indoor Atmosphere, Rhiannon De La Rosa, Mary Savin

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Sulfur dioxide is an inorganic compound (IC) and air pollutant that causes health risks in humans. The buildup of sulfur dioxide (SO2) in enclosed indoor spaces is, therefore, a concern to human health, especially since the average person spends 90% of his/her time indoors. This study focused on decreasing SO2 concentration in a cost-effective and simple way—by using botanical biofiltration, or the uptake of pollutants by plants. Research in biofiltration has focused mostly on the remediation of volatile organic compounds (VOC). However, research has also shown that plant species that remediate VOC efficiently also have the potential for efficient IC …

The Economics Of On-Farm Rice Drying In Arkansas, Clayton J. Parker, Lanier Nalley

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Globally, rice producers are faced with the temporal problem of deciding the optimal time to harvest rice. When harvested, paddy rice is typically at a harvest moisture content (HMC) between 15% and 22% and subsequently dried by the mill to a moisture content (MC) of 12.5%. Riceland Foods Inc., the largest miller of rice in the world, uses a stair-step pricing model to charge farmers to dry, which can complicate the timing of harvest as producers try to balance the tradeoff of minimizing drying costs by waiting to harvest at lower HMC vs. maintaining higher rice quality typically observed when …

Decomposition In Pasture Soil Receiving Excreta From Ruminants Fed Alfalfa Forage Diet Supplemented With Increasing Proportions Of Sericea Lespedeza Legume, Yang Kai Tang, Mary C. Savin, Dirk Philipp, Ken Coffey, Jiangchao Zhao

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Healthy soil is fundamental to a productive pasture system as it will decompose labile organic matter and promote retention of carbon to build a stable, resistant pool of organic matter. An easy, standardized approach to measure decomposition and litter stabilization that is gaining popularity in both citizen science and research studies is the use of the Tea Bag Index. The Tea Bag Index is a relatively new method evaluating the loss of organic material in two different kinds of commercial tea bags (green tea and Rooibos tea) after burial in the soil for 90 days. The objective of this experiment …

Corn Response To Wastewater-Recycled Phosphorus Fertilizers, Shane R. Ylagan, Kristofor R. Brye

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The ability to recycle phosphorus (P) from wastewaters could provide a sustainable, continuous source of P that might also help protect surface water quality from P enrichment. The mineral struvite (MgNH4PO4 · 6H2O) is an understudied material that can be created from P- and nitrogen (N)-containing wastewater and has been shown to have agricultural fertilizer value. The objective of this study was to evaluate the effects of electrochemically precipitated struvite (ECST), chemically precipitated struvite (Crystal Green; CG), diammonium phosphate (DAP), monoammonium phosphate (MAP), rock phosphate (RP), and triple superphosphate (TSP) on corn (Zea mays) response in a greenhouse pot study. …

Letter From Division Of Agriculture Communications, Fred Miller

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Evaluating The Impact Of Grafting On Local Tomato Production In Nebraska, Raihanah Hassim, Samuel E. Wortman, Ashley A. Thompson, Stacy A. Adams

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Grafting has been successfully used in vegetable production for tomatoes, peppers, eggplants, cucumbers, and watermelon. Besides its usefulness for managing soil-borne diseases, grafting can improve nutrient uptake and yield. However, few studies have assessed the effects of grafting and soil fertility management on yield of open field-grown tomatoes in the Midwest. Therefore, the objective of this two-year research was to better document the effects of grafting heirloom and hybrid tomato cultivar onto hybrid tomato rootstocks on tomato yield and quality. The field experiments were located at the University of Nebraska Lincoln - East Campus in Lincoln, West Central Research and …

Intercropping For Water Conservation: Environmental And Economic Implications Of A Sustainable Farming Practice In California's Central Valley, Sophie Baker

Scripps Senior Theses

California’s agricultural sector is the biggest water consumer in the state and faces intense pressure to reduce its overall water usage. Industrialized monoculture systems dominate the industry and often disregard long-term environmental and economic externalities for short-term profit maximization. To maintain longstanding food security and economic stability as well as protect the state’s water supply, it is critical that these systems transition to more sustainable and resilient production mechanisms. As an alternative to monoculture, intercropping affords greater potential to conserve water, protect soil quality, and increase crop yields, among other metrics of sustainability. However, there has been much controversy over …

Improving Kentucky's Winter Wheat And Double Crop Soybean Rotation, Katherine Susan Rod

Theses and Dissertations--Plant and Soil Sciences

The winter wheat double crop soybean rotation is an economically viable rotation for Kentucky farmers. Recent decreases in commodity prices has warranted the need to evaluate intensive management practices that can increase yields and profitability in this crop rotation. There were three goals of this dissertation: 1.) identify management practices that would decrease deoxynivalenol (DON) in harvested wheat grain and increase wheat heading and anthesis uniformity, 2.) evaluate double crop soybean planting timing and identify intensive management practices to increase seed yield, and 3.) determine the profitability of these management options. These studies were conducted in Princeton KY between 2016 …

Growth And Development, From: Forages: The Science Of Grassland Agriculture, Volume Ii, Robert B. Mitchell, Daren Redfearn, Kenneth J. Moore

Department of Agronomy and Horticulture: Faculty Publications

The growth and development of forage plants is an amazing process. In some annual grasses such as cereal rye, plants can go from the late vegetative stage to fully-flowered in less than two weeks. Conversely, some perennial grasses like indiangrass can go from the vegetative stage to the elongation stage, then enter a quiescent phase for several weeks until adequate moisture is available which then moves plants into the flowering stages to complete the seed production process. Understanding the developmental morphology of forage plants is important for making good management decisions. Many such decisions involve timing the initiation or termination …

Internet Of Things In Agricultural Innovation And Security, Abdul Salam

Computer Information Technology Faculty Publications

The agricultural Internet of Things (Ag-IoT) paradigm has tremendous potential in transparent integration of underground soil sensing, farm machinery, and sensor-guided irrigation systems with the complex social network of growers, agronomists, crop consultants, and advisors. The aim of the IoT in agricultural innovation and security chapter is to present agricultural IoT research and paradigm to promote sustainable production of safe, healthy, and profitable crop and animal agricultural products. This chapter covers the IoT platform to test optimized management strategies, engage farmer and industry groups, and investigate new and traditional technology drivers that will enhance resilience of the farmers to the …

Internet Of Things For Sustainable Forestry, Abdul Salam

Computer Information Technology Faculty Publications

Forests and grasslands play an important role in water and air purification, prevention of the soil erosion, and in provision of habitat to wildlife. Internet of Things has a tremendous potential to play a vital role in the forest ecosystem management and stability. The conservation of species and habitats, timber production, prevention of forest soil degradation, forest fire prediction, mitigation, and control can be attained through forest management using Internet of Things. The use and adoption of IoT in forest ecosystem management is challenging due to many factors. Vast geographical areas and limited resources in terms of budget and equipment …

Genetic Diversity Of Aflatoxin-Producing Aspergillus Flavus Isolated From Selected Groundnut Growing Agro-Ecological Zones Of Uganda, Amos Acur, Renee Arias, Steven Odongo, Samuel Tuhaise, National Agricultural Research Laboratories, Kampala, Uganda, John Adriko, Dennis Muhanguzi, Stephen Buah, Andrew Kiggundu

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Groundnut pre- and post-harvest contamination is commonly caused by fungi from the Genus Aspergillus. Aspergillus flavus is the most important of these fungi. It belongs to section Flavi; a group consisting of aflatoxigenic (A. flavus, A. parasiticus and A. nomius) and non-aflatoxigenic (A. oryzae, A. sojae and A. tamarii) fungi. Aflatoxins are food-borne toxic secondary metabolites of Aspergillus species associated with severe hepatic carcinoma and children stuntedness. Despite the well-known public health significance of aflatoxicosis, there is a paucity of information about the prevalence, genetic diversity and population structure of A. flavus in different …

Contributions In Puerto Rico To Bean, Phaseolus Spp., Research, James S. Beaver, Consuelo Estévez De Jensen, Phillip N. Miklas, Timothy G. Porch

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Most literature reviews focus on a specific topic. The purpose of this paper, however, is to review the contributions made by a multidisciplinary team of researchers at a specific location (Puerto Rico) over a period of several decades. This paper documents bean research of the University of Puerto Rico and the USDA-ARS Tropical Agriculture Research Station during the past century. The following illustrates the merits of continuity of effort in research and shows that research often follows a logical sequence of actions to solve problems related to genetic improvement as well as to study biotic and abiotic factors that affect …

The Morphological, Physiological, And Genetic Underpinnings Of Intraspecific Salinity Tolerance In Sorghum Bicolor, Ashley N. Henderson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Decreases in land quality and quantity threaten the efficient production of agriculturally and economically pivotal crops. Such reductions in arable lands are a consequence of population growth and urbanization, and often result in the introduction of various abiotic stresses. The most common abiotic stressors include water limitation (drought), water logging (over watering), poor water quality (salinity), and extreme temperatures (cold, frost, heat). Each of these stressors negatively impact plant growth, development, and yield. Soil salinity, specifically, is a considerable constraint affecting lands used in agriculture. Salts in the soil rise both naturally and through anthropogenic factors making the abundance a …

Comparing And Linking Organic Carbon And Iron In Soil And Headwater Stream In A Pasture And A Forest Catchment In A Central Appalachian Region, West Virginia, Lili Lei

Graduate Theses, Dissertations, and Problem Reports (ETD)

Continued global warming and surface water brownification are two main environmental issues which have attracted attention and are related to soil organic carbon (SOC) cycling. Iron oxides differ in reducibility and thus have essential roles in regulating SOC preservation and remineralization in soil and transport of dissolved organic carbon (DOC) from soil to surface water. In the central Appalachian region, anthropogenic disturbances are increasing, which leads to major issues of soil degradation and depletion of SOC concentrations. Cropland and pasture soils are subject to intense disturbances compared to the forest soil, which may lead to differences in SOC fractions and …

Integrated Crop–Livestock Systems In Paddy Fields: New Strategies For Flooded Rice Nutrition, Luiz Gustavo De O. Denardin, Amanda P. Martins, Felipe De C. Carmona, Murilo G. Veloso, Gabriela I. Carmona, Paulo César De F. Carvalho, Ibanor Anghinoni

Department of Entomology: Faculty Publications

Integrated crop–livestock systems (ICLSs) appear as a good alternative to increase nutrient use efficiency (NUE) in rice (Oryza sativa L.) through the improvement in nutrient cycling and soil chemical attributes in paddy fields. The objective of this study was to evaluate the impact of an ICLS on soil chemical attributes and on the fertilization requirement of N, P, and K by flooded rice in the Brazilian subtropical region. Nutritional status, yield, and NUE of flooded rice were evaluated by fertilization trials through rice response to different fertilization rates of N, P, and K. Soil chemical attributes were evaluated at …

Cover Crop Productivity And Subsequent Soybean Yield In The Western Corn Belt, Katja Koehler-Cole, Roger W. Elmore, Humberto Blanco-Canqui, Charles A. Francis, Charles Shapiro, Christopher A. Proctor, Sabrina J. Ruis, Derek M. Heeren, Suat Irmak, Richard Ferguson

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Cover crops (CC) in corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] rotations may prevent N loss and provide other ecosystem services but CC productivity in the western Corn Belt is limited by the short growing season. Our objective was to assess CC treatment and planting practice effects on CC biomass, spring soil nitrate concentrations, and soybean yield at two rainfed sites in eastern and one irrigated site in south-central Nebraska over 4 years. Cover crop treatments (cereal rye [Secale cereale L.] [RYE] and a mix of rye, legume, and brassica species [MIX]) were planted …

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Allelic Variation In Rice Fertilization Independent Endosperm 1 Contributes To Grain Width Under High Night Temperature Stress, Balpreet K. Dhatt, Puneet Paul, Jaspreet Sandhu, Waseem Hussain, Larissa Irvin, Feiyu Zhu, Maria Arlene Adviento-Borbe, Argelia Lorence, Paul Staswick, Hongfeng Yu, Gota Morota, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

A higher minimum (night-time) temperature is considered a greater limiting factor for reduced rice yield than a similar increase in maximum (daytime) temperature. While the physiological impact of high night temperature (HNT) has been studied, the genetic and molecular basis of HNT stress response remains unexplored.

We examined the phenotypic variation for mature grain size (length and width) in a diverse set of rice accessions under HNT stress. Genome-wide association analysis identified several HNT-specific loci regulating grain size as well as loci that are common for optimal and HNT stress conditions.

A novel locus contributing to grain width under HNT …

Voxel Carving-Based 3d Reconstruction Of Sorghum Identifies Genetic Determinants Of Light Interception Efficiency, Mathieu Gaillard, Chenyong Miao, James Schnable, Bedrich Benes

Department of Agronomy and Horticulture: Faculty Publications

Changes in canopy architecture traits have been shown to contribute to yield increases. Optimizing both light interception and light interception efficiency of agricultural crop canopies will be essential to meeting the growing food needs. Canopy architecture is inherently three-dimensional (3D), but many approaches to measuring canopy architecture component traits treat the canopy as a two-dimensional (2D) structure to make large scale measurement, selective breeding, and gene identification logistically feasible. We develop a high throughput voxel carving strategy to reconstruct 3D representations of sorghum from a small number of RGB photos. Our approach builds on the voxel carving algorithm to allow …

Genome-Wide Identification And Analysis Of Heterotic Loci In Three Maize Hybrids, Hongjun Liu, Qin Wang, Mengjiao Chen, Yahui Ding, Xuerong Yang, Jie Liu, Xiaohan Li, Congcong Zhou, Qilin Tian, Yiqi Lu, Danlin Fan, Junpeng Shi, Lin Zhang, Congbin Kang, Mingfei Mingfei Sun, Fangyuan Li, Yujian Wu, Yongzhong Zhang, Baoshen Liu, Xiang Yu Zhao, Qi Feng, Jinliang Yang, Bin Han, Jinsheng Lai, Xian Sheng Zhang, Xuehui Huang

Department of Agronomy and Horticulture: Faculty Publications

Heterosis, or hybrid vigour, is a predominant phenomenon in plant genetics, serving as the basis of crop hybrid breeding, but the causative loci and genes underlying heterosis remain unclear in many crops. Here, we present a large-scale genetic analysis using 5360 offsprings from three elite maize hybrids, which identifies 628 loci underlying 19 yield-related traits with relatively high mapping resolutions. Heterotic pattern investigations of the 628 loci show that numerous loci, mostly with complete–incomplete dominance (the major one) or overdominance effects (the secondary one) for heterozygous genotypes and nearly equal proportion of advantageous alleles from both parental lines, are the …

Cover Crops And Cover Crop Mixes: Stratification Of Biological Effects, Landon M. Gibbs

Theses and Dissertations--Plant and Soil Sciences

The potential nutrient cycling benefits from legumes (e.g. N₂-fixation) and the high biomass potential of cereal rye are well known. Further studies are warranted to evaluate bi-culture mixtures and their effects on soil nutrient stratification and microbial enzyme activity because these two properties may be differently expressed (enhanced) by legume/grass mixes. The objectives of this study were: (1) show different cover crops and cover crop mixes containing grasses and legumes differentially stratify carbon and N; (2) show the change in microbial enzyme activity in soils planted with individual cover crops relative to cover crop mixes; 3) determine the …

Effect Of Management Decisions On Corn Yield Productivity And Stability In Environments With Contrasting Water Availability, Juan Ignacio Di Salvo

Theses and Dissertations--Plant and Soil Sciences

Corn (Zea Mays L.) is a grain crop with large productivity, but also elevated evapotranspiration demand, making it highly susceptible to periods of water stress occurring during critical reproductive stages. Environmental conditions in Kentucky make it possible to grow corn under rainfed conditions, but the crop is still likely to experience water stress during some times of the growing season depending on the year and location. There is limited information on the size of the yield gap due to water stress in Kentucky, and the timing and intensity of water deficit.

In addition, evaluating the interactive effects of hybrid …

Effect Of Organic Soil Amendment On Hemp, Mounica Talasila

Murray State Theses and Dissertations

The research study is about the impact of different levels of organic soil amendment on the levels of psychoactive compounds produced in industrial hemp. Inorganic or chemical pesticides or fertilizers are not as extensively used in hemp as in other crops. Apart from this, using an organic soil amendment would be an environmentally sustainable way. A randomized block design (RBD) model was used to study the impacts of the soil amendment. The soil amendment used is called “Rehab” a product of Organilock created from the remnants of animals like fish and chicken. This research design has 3 different soil amendment …