Agronomy and Crop Sciences Commons^™

Laboratory Performance Of A Mass Flow Sensor For Dry Edible Bean Harvesters, Rodrigo S. Zandonadi, Timothy S. Stombaugh, Scott A. Shearer, Daniel M. Queiroz, Michael P. Sama

Biosystems and Agricultural Engineering Faculty Publications

Due to the importance of yield monitoring, researchers have been developing systems for crops such as tomatoes, forage, sugar cane, citrus, and coffee. A yield monitoring system for pull type dry edible beans harvester has not yet been developed. The goal of this project was to design and test a drive torque measurement device on a clean grain bucket elevator of a dry bean harvester, and evaluate its potential to be used as a mass flow sensor. Tests were conducted in the Yield Monitor Test Facility (YMTF) of University of Kentucky following the recommendation of ASABE Standard S578 (2007) The …

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Whole Genome Wide Expression Profiles Of Vitis Amurensis Grape Responding To Downy Mildew By Using Solexa Sequencing Technology, Jiao Wu, Yali Zhang, Huiqin Zhang, Hong Huang, Kevin M. Folta, Jiang Lu

School of Information Faculty Publications

Background: Downy mildew (DM), caused by pathogen Plasmopara viticola (PV) is the single most damaging disease of grapes (Vitis L.) worldwide. However, the mechanisms of the disease development in grapes are poorly understood. A method for estimating gene expression levels using Solexa sequencing of Type I restriction-endonuclease-generated cDNA fragments was used for deep sequencing the transcriptomes resulting from PV infected leaves of Vitis amurensis Rupr. cv. Zuoshan-1. Our goal is to identify genes that are involved in resistance to grape DM disease.

Results: Approximately 8.5 million (M) 21-nt cDNA tags were sequenced in the cDNA library derived from PV pathogen-infected …

Nebraska Seed Focus March 2010, Nebraska Crop Improvement Association

Nebraska Seed Focus

Seed Improvement Conference

The Distinguished Service Award

Correction to Seed Book

Nebraska Crop Improvement Association Annual Meeting Minutes

NCIA Board Reorganizes

Nebraska Seed Focus June 2010, Nebraska Crop Improvement Association

Nebraska Seed Focus

Wheat Seed Testing

Maps for Field Inspections

2010 Nebraska Winter Wheat Field Days

Nebraska Seed Focus September 2010, Nebraska Crop Improvement Association

Nebraska Seed Focus

Selling Seed in Bulk

New Insurance Product

Kansas Wheat Alliance Settles 3 PVP Infringement Cases

Sales Reports Due Dates

Inspected Acres 2010

Nebraska Seed Focus December 2010, Nebraska Crop Improvement Association

Nebraska Seed Focus

Nebraska Seed Improvement Conference - 2011

Tentative Schedule

Reminder

2011 Spring Seed Guides

OFFICIAL NOTICE

2010 Spring Seed Book, Nebraska Crop Improvement Association

Nebraska Crop Improvement Association Seed Guides

FOUNDATION SEED

PLANT VARIETY PROTECTION ACT and HOW IT BENEFITS YOU!

TURFGRASSES

PERENNIAL FORAGE GRASSES

SPRING WHEAT

SPRING BARLEY

OAT VARIETY CHARACTERISTICS - 2010

OATS

MILLET

HYBRID SEED CORN

NEBRASKA SEED QUALITY ASSURANCE® PROGRAM

SOYBEANS BUYERS’ NOTICE

APPROVED SEED CONDITIONERS

CUSTOM CERTIFIED CONDITIONERS

2009 NCIA MEMBERS

Genetic Diversity Of Wheat Cultivars From Turkey And U. S. Great Plains, Anyamanee Auvuchanon

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Genetic diversity of wheat cultivars from Turkey and the Great Plains was studied under the hypothesis ‘Turkey’ wheat originated from Turkey and is the original hard red winter wheat landrace in the Great Plains. Wheat cultivars in Turkey and the Great Plains were selected for adaptation in two countries which were similar in climate. Twenty-two Turkish and twenty-three Great Plains wheat cultivars were selected for this study using SSR markers, agronomic, and end-use quality traits data. Wheat cultivars were clustered into five groups based on SSR markers and the clustering largely followed their countries of origin and pedigree. Modern Great …

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 11 2010, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Letter From The Dean, Michael Vayda

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Yield Potential And Resource-Use Efficiency Of Maize Systems In The Western U. S. Corn Belt, Patricio Grassini

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Maize demand for food, livestock feed, and biofuel is expected to increase substantially. The Western U.S. Corn Belt accounts for 23% of U.S. maize production, and irrigated maize accounts for 43 and 58% of maize land area and total production, respectively, in this region. The most sensitive parameters (yield potential [YP], water-limited yield potential [YP-W], yield gap between actual yield and YP, and resource-use efficiency) governing performance of maize systems in the region are lacking. A simulation model was used to quantify YP under irrigated and rainfed conditions based on weather data, soil properties, and crop management at 18 locations. …

Nitrogen And Water Stress Impacts Hard Red Spring Wheat (Triticum Aestivum) Canopy Reflectance, Cheryl L. Reese, Daniel Long, David Clay, Sharon Clay, Dwayne Beck

Agronomy, Horticulture and Plant Science Faculty Publications

Remote sensing has been proposed as a method for implementing an inseason spring wheat (Triticum aestivum) nitrogen (N) fertilization program. However, in fields where yields are influenced by both water and N stress, accurate N recommendations require that that the N and water stress signals be separated from each other. The objective of this study was to determine the impact of water and N stress on canopy reflectance and the ability of vegetation (NDVI, GNDVI, and BNDVI), and chlorophyll (CGreen and CRedEdge) indices to separate water and N stress. A split-plot experiment containing four blocks was conducted in 2002, 2003, …

Phosphorus For High Rainfall Pastures, Mike Bolland, Bill Russell, David Weaver

Bulletins 4000 -

Soil testing for phosphorus provides a reliable indication of when the level of phosphorus in a soil is likely to be adequate for pasture production in the next growing season. It is a waste of money to apply phosphorus fertiliser when soil testing indicates none is required, or to acidified soils, or to undergrazed pastures, or to pastures dominated by poorly productive species.

Legumes have higher phosphorus requirement than grasses.

Critical soil test phosphorus values do not change when nitrogen fertiliser is applied.

Century-Old Mystery Of Puccinia Striiformis Life History Solved With The Identification Of Berberis As An Alternate Host, Yue Jin, Les J. Szabo, Martin Carson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The life history of Puccinia striiformis remains a mystery because the alternate host has never been identified. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P. striiformis. Analyses using real-time polymerase chain reaction and DNA sequence confirmed the rust fungus as P. striiformis. Pycnia and aecia were produced on B. chinensis, B. holstii, B. koreana, and B. vulgaris after inoculation using germinating telia of P. striiformis f. sp. tritici. Wheat inoculated with aeciospores from B. chinensis resulted in …

Agronomic Performance Of Five Varieties Of Forage Grasses Inroduced In Carmen Pampa, Province Of Nor Yungas, La Paz, Joel Eliseo Bautista Vargas

Theses and Dissertations

This study was done with the objective of evaluating the agronomic performance of five varieties of forage grasses introduced to the community of Carmen Pampa in the province of Nor Yungas, La Paz from 2005-2006. The average temperature during the study was 11.93ºC and the average precipitation was 801.75 mm. The trial was done with a design of randomized blocks having five species and six repetitions. The species studied were: Panicum virgatum, Sorghastrum nutans, Andropogon gerardii, Tripsacum dactyloides, and Eragrostis curvula. Variables evaluated were: plant height (cm), coverage (%), number of plants, bunches of florets, and dry matter yield (kg/ha). …

Arkansas Soybean Performance Tests 2009, D. G. Dombeck, R. D. Bond, I. L. Eldridge, R. M. Pryor

Arkansas Agricultural Experiment Station Research Series

Soybean variety and strain performance tests are conducted each year in Arkansas by the University of Arkansas Division of Agriculture Arkansas Crop Variety Improvement Program. The tests provide information to companies developing varieties and/or marketing seed within the state, and aid the Arkansas Cooperative Extension Service in formulating variety recommendations for soybean producers.

Grazing Evaluation Of A Novel Endophyte Tall Fescue Developed For The Upper Transition Zone, Jennifer Michelle Johnson

University of Kentucky Doctoral Dissertations

A wild-type endophyte (Neotyphodium coenophialum [(Morgan-Jones & Gams) Glen Bacon & Hanlin]) that infects tall fescue [Lolium arundinaceum (Schreb.) Darbysh. = Schedonorous arundinaceum (Schreb.) Dumort.] imparts tolerances to moisture, heat, and grazing stresses, but also produces ergot alkaloids that adversely affect performance and physiology of cattle. Novel endophytes, developed by AgResearch Ltd. NZ, can sustain fescue persistence and productivity, but do not produce toxic ergot alkaloids. University of Kentucky Plant Breeder, T. D. Phillips Ph.D, developed a tall fescue experimental population (KYFA9301) for the upper transition zone. A 2-yr grazing experiment was conducted with steers to evaluate steer …

A High Density Integrated Genetic Linkage Map Of Soybean And The Development Of A 1536 Universal Soy Linkage Panel For Quantitative Trait Locus Mapping, D. L. Hyten, Ik-Young Choi, Qijian Song, James E. Specht, Thomas E. Carter Jr., Randy C. Shoemaker, Eun-Young Hwang, Lakshmi K. Matukumalli, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Single nucleotide polymorphisms (SNPs) are the marker of choice for many researchers due to their abundance and the high-throughput methods available for their multiplex analysis. Only recently have SNP markers been available to researchers in soybean [Glycine max (L.) Merr.] with the release of the third version of the consensus genetic linkage map that added 1141 SNP markers to the map. Our objectives were to add 2500 additional SNP markers to the soybean integrated map and select a set of 1536 SNPs to create a universal linkage panel for high-throughput soybean quantitative trait locus (QTL) mapping. The GoldenGate assay …

Structural And Functional Divergence Of A 1-Mb Duplicated Region In The Soybean (Glycine Max) Genome And Comparison To An Orthologous Region From Phaseolus Vulgaris, Jer-Young Lin, Robert M. Stupar, Christian Hans, D. L. Hyten, Scott A. Jackson

Department of Agronomy and Horticulture: Faculty Publications

Soybean (Glycine max) has undergone at least two rounds of polyploidization, resulting in a paleopolyploid genome that is a mosaic of homoeologous regions. To determine the structural and functional impact of these duplications, we sequenced two ~1-Mb homoeologous regions of soybean, Gm8 and Gm15, derived from the most recent ~13 million year duplication event and the orthologous region from common bean (Phaseolus vulgaris), Pv5. We observed inversions leading to major structural variation and a bias between the two chromosome segments as Gm15 experienced more gene movement (gene retention rate of 81% in Gm15 versus 91% in …

Fine Mapping The Soybean Aphid Resistance Gene Rag1 In Soybean, Ki-Seung Kim, Stephanie Bellendir, Karen A. Hudson, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

The soybean aphid (Aphis glycines Matsumura) is an important soybean [Glycine max (L.) Merr.] pest in North America. The dominant aphid resistance gene Rag1 was previously mapped from the cultivar ‘Dowling’ to a 12 cM marker interval on soybean chromosome 7 (formerly linkage group M). The development of additional genetic markers mapping closer to Rag1 was needed to accurately position the gene to improve the effectiveness of markerassisted selection (MAS) and to eventually clone it. The objectives of this study were to identify single nucleotide polymorphisms (SNPs) near Rag1 and to position these SNPs relative to Rag1. …

Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from ‘Dowling’. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping …

An Integrative Approach To Genomic Introgression Mapping, Andrew J. Severin, Gregory A. Peiffer, Wayne W. Xu, D. L. Hyten, Bruna Bucciarelli, Jamie A. O'Rourke, Yung-Tsi Bolon, David Grant, Andrew Farmer, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker, Robert M. Stupar

Department of Agronomy and Horticulture: Faculty Publications

Near-isogenic lines (NILs) are valuable genetic resources for many crop species, including soybean (Glycine max). The development of new molecular platforms promises to accelerate the mapping of genetic introgressions in these materials. Here, we compare some existing and emerging methodologies for genetic introgression mapping: single-feature polymorphism analysis, Illumina GoldenGate single nucleotide polymorphism (SNP) genotyping, and de novo SNP discovery via RNA-Seq analysis of next-generation sequence data. We used these methods to map the introgressed regions in an iron-inefficient soybean NIL and found that the three mapping approaches are complementary when utilized in combination. The comparative RNA-Seq approach offers …

Crop Registration: The Pathway To Public Access Of Plant Genetic Materials To Build Crops For The Future, D. D. Ellis, K. A. Garland-Campbell, J. A. Grotenhuis, M. M. Jenderek, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Starting in 1926 and continuing for 80+ yr, 11,241 crop genetic materials have been registered as of 31 Dec. 2008. The crop registration process is an important pathway to publically describe and document new and useful genetic materials and to incorporate these into the public domain via the National Plant Germplasm System (NPGS). Crop Registration materials are now searchable via the Germplasm Resources Information Network (GRIN) and demand for registered materials remains strong with more than 9150 registered accessions distributed in the past 26 yr by the NPGS. Guidelines continue to evolve to accommodate global factors effecting germplasm exchange such …

Efficacy Of Singular And Stacked Brown Midrib 6 And 12 In The Modification Of Lignocellulose And Grain Chemistry, Scott E. Sattler, Deanna L. Funnell-Harris, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

In sorghum, brown midrib (bmr) 6 and 12 impair the last two steps of monolignol synthesis. bmr genes were introduced into grain sorghum to improve the digestibility of lignocellulosic tissues for grazing or bioenergy uses following grain harvest. Near-isogenic grain sorghum hybrids (AWheatland X RTx430) were developed containing bmr6, bmr12, and the bmr6 bmr12 double mutant (stacked), and their impacts were assessed in a two-year field study. The bmr genes did not significantly impact grain or lignocellulosic tissue yield. Lignocellulosic tissue from bmr6, bmr12, and stacked hybrids had reduced lignin content and increased in …

Heterosis In Sweet Sorghum And Selection Of A New Sweet Sorghum Hybrid For Use In Syrup Production In Appalachia, T. W. Pfeiffer, M. J. Bitzer, J. J. Toy, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Although heterosis is well established in grain and forage sorghum [Sorghum bicolor (L.) Moench], reports of heterosis in sweet sorghum are limited to results from grain sorghum × sweet sorghum hybrids. Recent development of cytoplasmic male-sterile sweet sorghum lines allows creation of sweet sorghum hybrids for research and industry. Male sterility may also affect allocation of photosynthate to plant parts, creating the potential to increase sugar content in stems by eliminating seed as a sink. The objectives of this study were to compare performance of A₃ cytoplasmic male-sterile lines and A₃ cytoplasmic male-sterile hybrids to fertile B …

Influences Of Wind And Sandblasting On The Endangered Blowout Penstemon, James Stubbendieck, Cheryl D. Dunn, Heidi L. Hillhouse, L. M. Landholt

Department of Agronomy and Horticulture: Faculty Publications

Blowout penstemon Penstemon haydenii is a federally endangered species growing only in areas with active wind erosion in sand dunes of the central United States. This early seral species declines as the blowout habitat stabilizes, allowing later seral species to increase. Blowout penstemon populations and plant size declined in the 1990s when precipitation was higher than normal, resulting in reduced sand movement. We conducted a greenhouse experiment to determine whether blowing sand influenced vigor and persistence of blowout penstemon seedlings. Treatments were wind, sandblasting, wind with sandblasting, and a control. The wind treatment was a constant 14 km h^{–1 …}

Fine Mapping Of The Scn Resistance Locus Rhg1-B From Pi 88788, Myungsik Kim, David L. Hyten, Andrew F. Bent, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is the most economically damaging soybean [Glycine max (L.) Merr.] pest in the USA and genetic resistance is a key component for its control. Although SCN resistance is quantitative, the rhg1 locus on chromosome 18 (formerly known as Linkage Group G) confers a high level of resistance. The objective of this study was to fi ne-map the rhg1-b allele that is derived from plant introduction (PI) 88788. F₂ and F₃ plants and F_3:4 lines from crosses between SCN resistant and susceptible genotypes were tested with genetic markers to …

High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Background: Next generation sequencing has significantly increased the speed at which single nucleotide polymorphisms (SNPs) can be discovered and subsequently used as molecular markers for research. Unfortunately, for species such as common bean (Phaseolus vulgaris L.) which do not have a whole genome sequence available, the use of next generation sequencing for SNP discovery is much more difficult and costly. To this end we developed a method which couples sequences obtained from the Roche 454-FLX system (454) with the Illumina Genome Analyzer (GA) for high-throughput SNP discovery. Results: Using a multi-tier reduced representation library we discovered a total of …