Open Access. Powered by Scholars. Published by Universities.®

Agronomy and Crop Sciences Commons™

Open Access. Powered by Scholars. Published by Universities.®

South Dakota State University

Discipline
Keyword
Publication Year
Publication
Publication Type

Articles 181 - 210 of 292

Full-Text Articles in Agronomy and Crop Sciences

Stability Analysis Of Immunogenic Gliadin Accumulation In Hard Red Spring Wheat (Triticum Aestivum L.), Stacy Lindblom-Dreis Jan 2018

Stability Analysis Of Immunogenic Gliadin Accumulation In Hard Red Spring Wheat (Triticum Aestivum L.), Stacy Lindblom-Dreis

Electronic Theses and Dissertations

Misconceptions regarding plant breeding objectives have led to speculation regarding the increasing prevalence of gluten intolerance, wheat allergy, and celiac disease. It is thought that contemporary wheat cultivars accumulate more immunogenic proteins than older cultivars because of supposed genetic changes effected through plant breeding strategies. This study evaluated the stability of gliadin accumulation in 191 hard red spring wheat cultivars grown in 12 location-years. Two enzyme-linked immunosorbent assays (ELISAs) were developed. A paired t test (N = 34) failed to find a statistically significant difference between the experimental ELISAs and the commercial R5 assay measures of mean gliadin (t = …


Soybean Aphid Biotype 4 Resistance In Soja And Soybean Plant Introductions, Sophia R. Conzemius Jan 2018

Soybean Aphid Biotype 4 Resistance In Soja And Soybean Plant Introductions, Sophia R. Conzemius

Electronic Theses and Dissertations

Soybean aphid, Aphis glycines Matsumura, is a major pest to soybean, Glycine max (L.) Merr. Host plant resistance is a management tactic that uses naturally occurring soybean plant defenses to limit soybean aphid pest damage. Virulent soybean aphid biotypes are able to successfully colonize on certain aphid resistant soybean. Soybean aphid biotype 4 is most virulent, overcoming all commercially available soybean aphid resistant soybeans (Rag1, Rag2, and Rag1+Rag2). Additional sources of resistance to avirulent biotypes have been identified in soja and soybean plant introductions (PIs). This study examined those resistant soja and soybean for resistance to the newly found soybean …


Regulation Of Local Auxin Metabolism During Soybean Nodule Development, Suresh Damodaran Jan 2018

Regulation Of Local Auxin Metabolism During Soybean Nodule Development, Suresh Damodaran

Electronic Theses and Dissertations

Legume-rhizobia symbiosis leads to the development of secondary root organs called nodules. Rhizobia bacteria housed inside nodules assimilate atmospheric nitrogen and convert them into plant usable forms thereby reducing the need for fertilizer application in crop legumes like soybean. Nodule development is a coordinated process orchestrated by multiple plant hormones. In soybean, the auxin responsive gene expression was detected in nodule primordia and in the periphery of mature nodules, primarily in nodule vasculature. Auxin hypersensitivity reduces nodule formation in soybean and also polar auxin transport inhibition at the site of nodule development is not crucial for determinate nodule formation. Therefore, …


Quantifying The Short-Term Impacts Of Cover Crops And Grazing On Soil Health Under An Integrated Crop-Livestock System In South Dakota, Vishal Seth Jan 2018

Quantifying The Short-Term Impacts Of Cover Crops And Grazing On Soil Health Under An Integrated Crop-Livestock System In South Dakota, Vishal Seth

Electronic Theses and Dissertations

Cover crops and grazing under Integrated Crop-Livestock System (ICLS) can impact the rhizosphere nutrient cycling and may have potential to increase the long-term sustainability and economic profitability of agricultural production system. In South Dakota, crop production practices and livestock husbandry are both common which provide the opportunity for ICLS to be successful in this region. However, little is known about the impacts of the cover crops and grazing under ICLS on soil properties in this region. The present study was conducted at South Dakota State University Research Farm (44°20'34.8"N, 96°48'14.8"W), Brookings, SD, to quantify the impacts of cover crops and …


Palmer Amaranth In South Dakota: Growth, Herbicidal Control, And Soybean Yield Loss, Brian Van De Stroet Jan 2018

Palmer Amaranth In South Dakota: Growth, Herbicidal Control, And Soybean Yield Loss, Brian Van De Stroet

Electronic Theses and Dissertations

Palmer amaranth is a growing concern in the United States. Previously thought to only be able to occupy the southern United States, this plant can now be found throughout the northern states as well. Infestations of Palmer amaranth can now be found in South Dakota and is raising many concerns. Palmer amaranth is characterized by large growth and can be highly competitive with many important crops. Soybean is an important crop in South Dakota, as well as the rest of the world, and has not escaped the detrimental aspects of an infestation of Palmer amaranth. The objectives of this study …


A Public–Industry Partnership For Enhancing Corn Nitrogen Research And Datasets: Project Description, Methodology, And Outcomes, Newell R. Kitchen, John F. Shanahan, Curtis J. Ransom, Christopher J. Bandura, Gregory M. Bean, James J. Camberato, Paul R. Carter, Jason D. Clark, Richard B. Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A.M. Laboski, Emerson D. Nafziger, Zhisheng Qing, John E. Sawyer, Matt Shafer Aug 2017

A Public–Industry Partnership For Enhancing Corn Nitrogen Research And Datasets: Project Description, Methodology, And Outcomes, Newell R. Kitchen, John F. Shanahan, Curtis J. Ransom, Christopher J. Bandura, Gregory M. Bean, James J. Camberato, Paul R. Carter, Jason D. Clark, Richard B. Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A.M. Laboski, Emerson D. Nafziger, Zhisheng Qing, John E. Sawyer, Matt Shafer

Agronomy, Horticulture and Plant Science Faculty Publications

Due to economic and environmental consequences of N lost from fertilizer applications in corn (Zea mays L.), considerable public and industry attention has been devoted to the development of N decision tools. Needed are research and databases and associated metadata, at numerous locations and years to represent a wide geographic range of soil and weather scenarios, for evaluating tool performance. The goals of this research were to conduct standardized corn N rate response field studies to evaluate the performance of multiple public-domain N decision tools across diverse soils and environmental conditions, develop and publish new agronomic science for improved …


Biomass Yield Of Switchgrass Cultivars Under High- Versus Low-Input Conditions, Michael D. Casler, Sergio Sosa, Lindsey Hoffman, Hilary Mayton, Calvin Ernst, Paul R. Adler, Arvid R. Boe, Stacy A. Bonos Mar 2017

Biomass Yield Of Switchgrass Cultivars Under High- Versus Low-Input Conditions, Michael D. Casler, Sergio Sosa, Lindsey Hoffman, Hilary Mayton, Calvin Ernst, Paul R. Adler, Arvid R. Boe, Stacy A. Bonos

Agronomy, Horticulture and Plant Science Faculty Publications

Switchgrass (Panicum virgatum L.) is undergoing development as a biomass crop to support conversion of cellulosic biomass to energy. To avoid the competition of biomass with food or feed crops, most commercialization proposals suggest that switchgrass should be grown exclusively on marginal lands that are not fit for food or feed production. The objective of this study was to investigate the potential for cultivar x environment interactions that would affect the methods and approaches for breeding and evaluating switchgrass cultivars, including both upland and lowland types, for high-input versus low-input types of environments. Biomass yield was measured on 14 cultivars …


Corn Silage Variety Trial Archive, Jonathan Kleinjan, David Casper, Kevin K. Kirby, S. M. Hawks Jan 2017

Corn Silage Variety Trial Archive, Jonathan Kleinjan, David Casper, Kevin K. Kirby, S. M. Hawks

SDSU Extension Publications Archive

This report features the available corn silage data from 2003-2017. Crop performance testing results are released annually through the activities of SDSU Extension and the South Dakota Agricultural Experiment Station at SDSU.


Evaluating Adaptions Of Soft Red Winter Wheat In Eastern Region Of Usa, Dilmini Alahakoon, Anne Fennell, Jixiang Wu Jan 2017

Evaluating Adaptions Of Soft Red Winter Wheat In Eastern Region Of Usa, Dilmini Alahakoon, Anne Fennell, Jixiang Wu

Agronomy, Horticulture and Plant Science Faculty Publications

Identification of winter wheat genotypes that are highly adapted to a wide range of environmental conditions is one of the most important wheat research objectives. Multienvironment trials (METs) under diverse environments is a commonly used practice to evaluate mean performance and yield stability. However, locations used and genotypes planted may vary from year to year which may cause yield stability analysis to be statistically challenging. In this study, we evaluated yield trial data containing 117 eastern soft red winter wheat genotypes that were grown in 35 locations in eastern production areas and four growing seasons (2012/2013 to 2015/2016). We used …


Spring Wheat Variety Trial Archive, Robert Hall, Kevin Kirby, John Rickertsen, Bruce Swan, G. Piechowski, Jesse Hall, Nathan Mueller, Christopher Graham, S. M. Hawks, Jonathan Kleinjan Jan 2017

Spring Wheat Variety Trial Archive, Robert Hall, Kevin Kirby, John Rickertsen, Bruce Swan, G. Piechowski, Jesse Hall, Nathan Mueller, Christopher Graham, S. M. Hawks, Jonathan Kleinjan

SDSU Extension Publications Archive

Crop performance testing results are released annually through the activities of SDSU Extension and the South Dakota Agricultural Experiment Station at SDSU.


Oats Variety Trial Archive, Robert Hall, Kevin K. Kirby, John Rickertsen, Bruce Swan, G. Piechowski, Jesse Hall, S. M. Hawks, Nathan Mueller, Christopher Graham, Jonathan Kleinjan, Shaukat Ali Jan 2017

Oats Variety Trial Archive, Robert Hall, Kevin K. Kirby, John Rickertsen, Bruce Swan, G. Piechowski, Jesse Hall, S. M. Hawks, Nathan Mueller, Christopher Graham, Jonathan Kleinjan, Shaukat Ali

SDSU Extension Publications Archive

This report features the available oats data from 2003-2017. Crop performance testing results are released annually through the activities of SDSU Extension and the South Dakota Agricultural Experiment Station at SDSU.


Grain Sorghum Variety Trial Archive, John Rickertsen, Bruce Swan, Nathan Mueller, Christopher Graham, Jonathan Kleinjan, Kevin Kirby, Dwayne Beck, Michael Swan Jan 2017

Grain Sorghum Variety Trial Archive, John Rickertsen, Bruce Swan, Nathan Mueller, Christopher Graham, Jonathan Kleinjan, Kevin Kirby, Dwayne Beck, Michael Swan

SDSU Extension Publications Archive

This report features the available sorghum data from 2003-2017. Crop performance testing results are released annually through the activities of SDSU Extension and the South Dakota Agricultural Experiment Station at SDSU.


Sunflower & Flax Hybrid Performance And Variety Trial Archive, Kathleen Grady, Lee Gilbertson, Thandiwe Nleya, John Rickertsen, Bruce Swan, Febina Mathew, Christopher Graham, Nathan Braun, Paul Okello Jan 2017

Sunflower & Flax Hybrid Performance And Variety Trial Archive, Kathleen Grady, Lee Gilbertson, Thandiwe Nleya, John Rickertsen, Bruce Swan, Febina Mathew, Christopher Graham, Nathan Braun, Paul Okello

SDSU Extension Publications Archive

This report features the available sunflower and flax data from 2003-2017. Crop performance testing results are released annually through the activities of SDSU Extension and the South Dakota Agricultural Experiment Station at SDSU.


Alfalfa Variety Trial Results Archive, Vance Owens, R. Bortnem, D. Gustafson, E. Omdahl, Chris Lee, Peter Jeranyama, Matt Hansen, Karla Hernandez Jan 2017

Alfalfa Variety Trial Results Archive, Vance Owens, R. Bortnem, D. Gustafson, E. Omdahl, Chris Lee, Peter Jeranyama, Matt Hansen, Karla Hernandez

SDSU Extension Publications Archive

Crop performance testing results are released annually through the activities of SDSU Extension and the South Dakota Agricultural Experiment Station at SDSU.


Soybean Variety Trial Archive, Kevin Kirby, Robert Hall, G. Piechowski, Jesse Hall, S. M. Hawks, Nathan Mueller, Jonathan Kleinjan Jan 2017

Soybean Variety Trial Archive, Kevin Kirby, Robert Hall, G. Piechowski, Jesse Hall, S. M. Hawks, Nathan Mueller, Jonathan Kleinjan

SDSU Extension Publications Archive

This report features the available soybean variety trial data from 2003-2017. Crop performance testing results are released annually through the activities of SDSU Extension and the South Dakota Agricultural Experiment Station at SDSU.


Corn Hybrid Trial Archive, Robert Hall, Kevin Kirby, G. Piechowski, Nathan Mueller, Jonathan Kleinjan, Jesse Hall, S. M. Hawks Jan 2017

Corn Hybrid Trial Archive, Robert Hall, Kevin Kirby, G. Piechowski, Nathan Mueller, Jonathan Kleinjan, Jesse Hall, S. M. Hawks

SDSU Extension Publications Archive

This report features the available corn data from 2003-2017. Crop performance testing results are released annually through the activities of SDSU Extension and the South Dakota Agricultural Experiment Station at SDSU.


Phytophthora Sojae Infecting Soybean: Pathotype Diversity, New Sources Of Resistance And Interaction With The Soybean Cyst Nematode, Rawnaq Nazneen Chowdhury Jan 2017

Phytophthora Sojae Infecting Soybean: Pathotype Diversity, New Sources Of Resistance And Interaction With The Soybean Cyst Nematode, Rawnaq Nazneen Chowdhury

Electronic Theses and Dissertations

Phytophthora root and stem rot, caused by Phytophthora sojae Kaufmann and Gerdemann, is an important disease of soybean (Glycine max L.) in South Dakota. To gain a better understanding of the importance of P. sojae in South Dakota, specifically pathotype diversity, identification of new resistance sources and the interaction with the soybean cyst nematode (Heterodera glycines Ichinohe, SCN), this research was undertaken with the following objectives - 1) to characterize the pathotype diversity of P. sojae causing Phytophthora root and stem rot on soybean in commercial fields in South Dakota; 2) to compare inoculation methods to evaluate for …


Using Gluten Index To Improve Spring Wheat Loaf Volume Prediction, Yaming Lu Jan 2017

Using Gluten Index To Improve Spring Wheat Loaf Volume Prediction, Yaming Lu

Electronic Theses and Dissertations

Wheat (Triticum aestivum L.) is the most widely cultivated crop in the world and contributes about 20% of the total dietary calories and proteins globally. Unique properties of doughs formed from wheat flour make it feasible to produce a range of food, including bread. Loaf volume is the most perceptible indicator for breadmaking quality. Selection in the early generations for loaf volume is difficult due to the requirement of the large volume of grains and due to the costly, time-consuming, and labor-intensive evaluation process. Identification of simple and reliable predictive tests for loaf volume is highly desirable. This study …


Mitigating Risks Of Ochratoxin A Contamination In Oats, Bandana Dhungana Jan 2017

Mitigating Risks Of Ochratoxin A Contamination In Oats, Bandana Dhungana

Electronic Theses and Dissertations

Ochratoxin A (OTA) is a toxic metabolite produced by several species of fungal genera Penicillium and Aspergillus. The major OTA producer in temperate regions (i.e. U.S., Canada, Europe) is Penicillium verrucosum. Oat (Avena sativa), like any other cereal grain, can be contaminated with OTA when storage conditions are favorable for fungal growth. The presence of OTA exceeding the European Union maximum limit of 3 ppb in processed food has been reported in some samples of oat-based breakfast cereals from the US. The use of oat genotypes with limited OTA accumulation would be an effective way to …


Soil Response To Cropping Sequences And Grazing Under Integrated Crop-Livestock System, Hanxiao Feng Jan 2017

Soil Response To Cropping Sequences And Grazing Under Integrated Crop-Livestock System, Hanxiao Feng

Electronic Theses and Dissertations

Integrated crop-livestock system (ICLS) is helpful in diversifying a farm for improving its long-term sustainability and economic benefits. In the United States, the ICLS has been increasing in recent years because of its economic and environmental benefits. However, the impacts of ICLS on soil quality is not well documented in North Dakota. The objective of our study is to assess the impacts of cropping sequences and cattle grazing on the selected soil properties in the crop diversity and livestock integration practice. This study site was established in 2010 at the Dickinson Research Extension Center, Dickinson, North Dakota. The study design …


Molecular Characterization Of Spot Blotch And Bacterial Leaf Streak Resistance In Bread Wheat, Girma Ayana Jan 2017

Molecular Characterization Of Spot Blotch And Bacterial Leaf Streak Resistance In Bread Wheat, Girma Ayana

Electronic Theses and Dissertations

Spot blotch (SB), caused by Bipolaris sorokiniana (Sacc.) Shoem, and bacterial leaf streak (BLS), caused by Xanthomonas translucens pv. undulosa (Smith et al.), two important foliar diseases of wheat in the major production regions of the US and the world. Deployment of adequate host resistance against them depends on determining the resistance genes or quantitative trait loci (QTLs) responsible for the trait and identification of genetic markers linked to QTL that facilitate markers assisted breeding. We conducted two independent studies and characterized QTLs for BLS and SB resistance. In the first study, we constructed a genetic linkage map of 1,211 …


Characterization Of Pyrenophora Tritici-Repentis In Wheat And Rye To Study Tan Spot Susceptibility And Insights Into Its Relationship With Stem Rust Resistance, Sidrat Abdullah Jan 2017

Characterization Of Pyrenophora Tritici-Repentis In Wheat And Rye To Study Tan Spot Susceptibility And Insights Into Its Relationship With Stem Rust Resistance, Sidrat Abdullah

Electronic Theses and Dissertations

Tan spot, caused by the ascomycete fungus, Pyrenophora tritici-repentis (Ptr), is a major foliar fungal disease of wheat worldwide. To date, Ptr isolates have been grouped into eight races based on the production of three host selective effectors (toxins), Ptr ToxA, ToxB, and ToxC, which are associated with necrosis and chlorosis symptoms on wheat differential lines. In order to see the correlation between stem rust resistance and tan spot susceptibility in 1970’s, to know the pathogen virulence profile, and explore the sources of tan spot resistance, we recovered isolates from wheat and rye and evaluated wheat and rye lines with …


Interactive Effects Of Cover Crops, Invertebrate Communities And Soil Health In Corn Production Systems, Claire Lacanne Jan 2017

Interactive Effects Of Cover Crops, Invertebrate Communities And Soil Health In Corn Production Systems, Claire Lacanne

Electronic Theses and Dissertations

The adoption of regenerative farming practices is gaining traction, but the costs and benefits are not often considered on a systems level. Encouraging biodiversity and soil health is the goal of many agricultural practices used in regenerative farming; regenerative systems employ practices which abide by the two main principles of increasing biodiversity and decreasing disturbance, with the goal of encouraging ecosystem functioning to minimize inputs and maximize the productivity of a farm. I examined the management of corn (Zea mays) fields across four states in the Upper Midwest region of the United States. Regenerative systems in this study …


Field Pea Variety Trial Archive, Robert Hall, John Rickertsen, Kevin K. Kirby, Bruce Swan, G. Piechowski, Jesse Hall, Nathan Mueller, Ruth Beck, Christopher Graham, Jonathan Kleinjan, S. M. Hawks, Michael Swan Jan 2017

Field Pea Variety Trial Archive, Robert Hall, John Rickertsen, Kevin K. Kirby, Bruce Swan, G. Piechowski, Jesse Hall, Nathan Mueller, Ruth Beck, Christopher Graham, Jonathan Kleinjan, S. M. Hawks, Michael Swan

SDSU Extension Publications Archive

This report features the available pea data from 2003-2017. Crop performance testing results are released annually through the activities of SDSU Extension and the South Dakota Agricultural Experiment Station at SDSU.


Development Of Best Management Practices For Production Of Ethiopian Mustard (Brassica Carinata) In South Dakota, Phillip Kenneth Alberti Jan 2017

Development Of Best Management Practices For Production Of Ethiopian Mustard (Brassica Carinata) In South Dakota, Phillip Kenneth Alberti

Electronic Theses and Dissertations

Ethiopian mustard (Brassica carinata) is an alternative non-food oilseed crop which has received interest for its potential as a low-input option for production in the Northern Great Plains (NGP). As B. carinata is a new crop to the NGP, the best management practices have yet to be developed. The overall goal of this project was to develop best management practices for B. carinata production in two diverse agroenvironments in South Dakota. Two field experiments were conducted to assess the response of two B. carinata varieties to i) four seeding rates (4.5, 9, 13.5, and 18 kg ha-1 …


Physiological And Molecular Characterisation Of Lucerne (Medicago Sativa L.) Germplasm With Improved Seedling Freezing Tolerance, M. Rokebul Anowar, Anne Fennell, Arvid Boe, Ivan W. Mott, Michael D. Peel, Yajun Wu Jun 2016

Physiological And Molecular Characterisation Of Lucerne (Medicago Sativa L.) Germplasm With Improved Seedling Freezing Tolerance, M. Rokebul Anowar, Anne Fennell, Arvid Boe, Ivan W. Mott, Michael D. Peel, Yajun Wu

Agronomy, Horticulture and Plant Science Faculty Publications

We conducted greenhouse experiments to compare 14 lucerne (alfalfa, Medicago sativa L.) germplasms for their survival following freezing. Some are collections adapted to the Grand River National Grasslands in South Dakota. We hypothesised that these collections might have developed a tolerance to survive the frigid growth conditions common there. Two of these collections, River side (RS) and Foster ranch (FR), showed greater freezing tolerance than the other germplasms tested, based on their consistent survival rates with or without cold acclimation. In multiple freezing studies, RS and FR had average survival rates of 74% and 79%, respectively, in contrast to the …


Igrow Corn: Best Management Practices, David E. Clay, Gregg G. Carlson, Sharon A. Clay, Emmanuel Byamukama Jan 2016

Igrow Corn: Best Management Practices, David E. Clay, Gregg G. Carlson, Sharon A. Clay, Emmanuel Byamukama

Agronomy, Horticulture, and Plant Science Books

No abstract provided.


Biochar Reduced Nitrous Oxide And Carbon Dioxide Emissions From Soil With Different Water And Temperature Cycles, Jiyul Chang, David E. Clay, Sharon A. Clay, Rajesh Chintala, Janet M. Miller, Thomas Schumacher Jan 2016

Biochar Reduced Nitrous Oxide And Carbon Dioxide Emissions From Soil With Different Water And Temperature Cycles, Jiyul Chang, David E. Clay, Sharon A. Clay, Rajesh Chintala, Janet M. Miller, Thomas Schumacher

Agronomy, Horticulture and Plant Science Faculty Publications

Interactions among biochar, respiration, nitrification, and soils can result in biochar increasing, decreasing, or not impacting greenhouse gas (GHG) emissions. This experiment determined the impact of water-filled porosity (WFP) and corn (Zea mays L.) stover biochar on CO2 and N2O emissions in May (spring) and August (summer). The May experiment contained two N rates [0 and 224 kg Ca(NO3)2–N ha–1], whereas the August had three N rates [0, 224 kg Ca(NO3)2–N ha–1, and 224 kg (NH4)2SO4–N ha–1 …


Soil Salinity Study In Northern Great Plains Sodium Affected Soil, Tulsi P. Kharel Jan 2016

Soil Salinity Study In Northern Great Plains Sodium Affected Soil, Tulsi P. Kharel

Electronic Theses and Dissertations

Climate and land-use changes when combined with the marine sediments that underlay portions of the Northern Great Plains have increased the salinization and sodification risks. The objectives of this dissertation were to compare three chemical amendments (calcium chloride, sulfuric acid and gypsum) remediation strategies on water permeability and sodium (Na) transport in undisturbed soil columns and to develop a remote sensing technique to characterize salinization in South Dakota soils. Fortyeight undisturbed soil columns (30 cm x 15 cm) collected from White Lake, Redfield, and Pierpont were used to assess the chemical remediation strategies. In this study the experimental design was …


Restoration Of Eroded Lands With Biochar As Soil Amendment In South Dakota, Saroop S. Sandhu Jan 2016

Restoration Of Eroded Lands With Biochar As Soil Amendment In South Dakota, Saroop S. Sandhu

Electronic Theses and Dissertations

Biochar is produced from incomplete pyrolysis of plant biomass. Due to the unique properties of biochar such as a higher number of micropores, surface area and carbon content, and recalcitrant nature of carbon, biochar is hypothesized to improve physical and hydrological properties of soil. This study assesses the impact of biochar on soil organic carbon, physical and hydrological properties of two landscape positions under a corn (Zea mays L.)-soybean (Glycine max L.) rotation. Three types of biochar including corn stover, ponderosa pine (Pinus ponderosa Lawson and C. Lawson) wood residue, and switchgrass (Panicum virgatum L.) were applied in the field …