Agronomy and Crop Sciences Commons^™

Soybean Barcsoysnp6k: An Assay For Soybean Genetics And Breeding Research, Qijian Song, Long Yan, Charles Quigley, Edward Fickus, He Wei, Linfeng Chen, Faming Dong, Susan Araya, Jinlong Liu, David Hyten, Vincent R. Pantalone, Randall L. Nelson

Department of Agronomy and Horticulture: Faculty Publications

The limited number of recombinant events in recombinant inbred lines suggests that for a biparental population with a limited number of recombinant inbred lines, it is unnecessary to genotype the lines with many markers. For genomic prediction and selection, previous studies have demonstrated that only 1000–2000 genome-wide common markers across all lines/accessions are needed to reach maximum efficiency of genomic prediction in populations. Evaluation of too many markers will not only increase the cost but also generate redundant information. We developed a soybean (Glycine max) assay, BARCSoySNP6K, containing 6000 markers, which were carefully chosen from the SoySNP50K assay based …

Functional Characterization Of Petiolule-Like Pulvinus (Plp) Gene In Abscission Zone Development In Medicago Truncatula And Its Application To Genetic Improvement Of Alfalfa, Juan Du, Shaoyun Lu, Maofeng Chai, Chuanen Zhou, Liang Sun, Yuhong Tang, Jaydeep Kolape, Zhaozhu Wen, Marjan Behzadirad, Tianxiu Zhong, Juan Sun, Yunwei Zhang, Zeng-Yu Wang

Department of Agronomy and Horticulture: Faculty Publications

Alfalfa (Medicago sativa L.) is one of the most important forage crops throughout the world. Maximizing leaf retention during the haymaking process is critical for achieving superior hay quality and maintaining biomass yield. Leaf abscission process affects leaf retention. Previous studies have largely focused on the molecular mechanisms of floral organ, pedicel and seed abscission but scarcely touched on leaf and petiole abscission. This study focuses on leaf and petiole abscission in the model legume Medicago truncatula and its closely related commercial species alfalfa. By analysing the petiolule-like pulvinus (plp) mutant in M. truncatula at phenotypic level (breakstrength and shaking …

B.R. Wells Arkansas Rice Research Studies 2019, K.A. K. Moldenhauer, B. Scott, J. Hardke

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Influence Of Cover Crop Management Practices On Rainfed Corn Production In Semi-Arid Western Nebraska, Alexandre Tonon Rosa

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

With the increased cover crop (CC) popularity, producers of semi-arid regions of western Nebraska are questioning whether they could successfully incorporate CC into their rainfed winter wheat (Triticum aestivum L.)-corn (Zea mays L.)-fallow rotations. The major concern is that CCs may deplete soil water affecting the subsequent crop. Therefore, three studies were established under rainfed conditions of western Nebraska to access the effects of CCs on soil water, soil compaction, nutrient cycling, weed demographics, residue coverage, and subsequent corn yield. The first study evaluated the influence of CC planting and termination times prior to corn establishment. Late termination …

Perioperative And Oncological Outcomes Following Minimally Invasive Versus Open Pancreaticoduodenectomy For Pancreatic Duct Adenocarcinoma, Rui Sun, Jiawen Yu, Yifan Zhang

Department of Agronomy and Horticulture: Faculty Publications

Background The outcomes of minimally invasive pancreaticoduodenectomy have not been adequately compared with those of open pancreaticoduodenectomy in patients with pancreatic ductal adenocarcinoma. We performed a meta‐analysis to compare the perioperative and oncological outcomes of these two pancreaticoduodenectomy procedures specifically in patients with pancreatic ductal adenocarcinoma.

Methods Before this study was initiated, a specific protocol was designed and has been registered in PROSEPRO (ID: CRD42020149438). Using the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines, PubMed, EMBASE, Web of Science, Cochrane Central Register, and ClinicalTrials.gov databases were systematically searched for studies published between January 1994 and October 2019. …

Evaluating Opportunities For Integrated Crop Livestock Systems In Eastern Nebraska, Elizabeth Kay Christenson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Current agricultural systems focused on production of few commodities are facing production, economic, and environmental challenges. To address these challenges, Integrated Crop Livestock Systems (ICLS) have emerged through three primary methods 1) perennial grasslands for grazing and/or hay production, 2) crop residue grazing, and 3) cover crop grazing. To evaluate potential of ICLS mitigating current challenges, a field scale model ICLS was developed in 2015. The ICLS includes 4-ha each of ‘Newell’ smooth bromegrass (Bromus inermis L.), ‘Liberty’ switchgrass (Panicum virgatum L.), and ‘Shawnee’ switchgrass. The ICLS also included 8-ha of continuous corn (Zea mays L.). In …

Soil-Nitrogen, Potentially Mineralizable-Nitrogen, And Field Condition Information Marginally Improves Corn Nitrogen Management, Jason Clark, Fabián G. Fernández, Kristen S. Veum, James J. Camberato, Paul R. Carter, Richard Ferguson, David W. Franzen, Daniel E. Kaiser, Newell R. Kitchen, Carrie A.M. Laboski, Emerson Nafziger, Carl J. Rosen, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Anaerobic potentially mineralizable nitrogen (PMN) combined with preplant nitrate test (PPNT) or pre-sidedress nitrate test (PSNT) may improve corn (Zea mays L.) N management. Forty-nine corn N response studies were conducted across the U.S. Midwest to evaluate the capacity of PPNT and PSNT to predict grain yield, N uptake, and economic optimal N rate (EONR) when adjusted by soil sampling depth, soil texture, temperature, PMN, and initial NH₄–N from PMN analysis. Pre-plant soil samples were obtained for PPNT (0- to 30-, 30- to 60-, 60- to 90-cm depths) and PMN (0- to 30-cm depth) before corn planting …

Arkansas Corn And Grain Sorghum Research Studies 2019, Victor Ford, Jason Kelley, Nathan Mckinney Ii

Arkansas Agricultural Experiment Station Research Series

The 2020 Arkansas Corn and Grain Sorghum Research Studies Series is the inaugural edition of this annual report and includes research results on all topics pertaining to corn and grain sorghum production including disease management, environmental/sustainability, irrigation, post-harvest drying, soil fertility, weed control, and research verification program results. Our objective is capturing and broadly distributing the results of research projects funded by the Arkansas Corn and Grain Sorghum Board. The intended audience includes producers and their advisors, current investigators, and future researchers. The Series will also serve as a citable archive of research results. Research reports contained in this publication …

Phenotypic And Genotypic Studies In The Peach [Prunus Persica (L.) Batsch] And Muscadine Grape (Vitis Rotundifolia Michx.), Jennifer Anne Lewter

Graduate Theses and Dissertations

Peach: Peaches [Prunus persica (L.) Batsch] are routinely chilled to increase shelf-life. Exposure to temperatures of 5° C for two weeks can induce chilling injury (CI) symptoms, including flesh mealiness (or wooliness) and a lack of juiciness. Phenotypic data were collected on seven biparental F1 peach populations maintained at the University of Arkansas Fruit Research Station. A genome wide association study (GWAS) was performed using TASSEL 5 which identified four quantitative trait loci (QTLs) associated with expressible juice, four QTLs for mealiness, five QTLs for soluble solids, and three QTLs for fruit weight. Exploiting these genetic markers could help breeders …

Effects Of Pyric Herbivory On Prairie-Chicken (Tympanuchus Spp) Habitat, Heath D. Starns, Samuel D. Fuhlendorf, Robert D. Elmore, Dirac Twidwell, Eric T. Thacker, Torre J. Hovick, Barney Luttbeg

Department of Agronomy and Horticulture: Faculty Publications

The reduction and simplification of grasslands has led to the decline of numerous species of grassland fauna, particularly grassland-obligate birds. Prairie-chickens (Tympanuchus spp.) are an example of obligate grassland birds that have declined throughout most of their distribution and are species of conservation concern. Pyric herbivory has been suggested as a land management strategy for enhancing prairie-chicken habitat and stabilizing declining population trends. We assessed differences in vegetation structure created by pyric herbivory compared to fire-only treatments to determine whether pyric herbivory increased habitat heterogeneity for prairie-chickens, spatially or temporally. Our study was performed at four sites in the southern …

Production And Selection Of Quality Protein Popcorn Hybrids Using A Novel Ranking System And Combining Ability Estimates, Leandra Parsons, Ying Ren, Abou Yobi, Preston Hurst, Ruthie Angelovici, Oscar Rodriguez, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Popcorn varieties are agronomically sub-optimal and genetically limited compared to other maize subspecies. To increase genetic diversity and improve popcorn agronomics, dent germplasm has been introduced to popcorn with limited success and generally, major loss of popping. Between 2013 and 2018, 12 Quality Protein Popcorn (QPP) inbreds containing Quality Protein Maize (QPM) and popcorn germplasm were produced that maintained popping while carrying the opaque-2 allele conferring elevated kernel lysine. This is an opportune trait in the growing market for healthier snacks and a model for mining QPM traits into popcorn. We crossed QPP inbreds to explore the effects of heterosis …

Genomic Prediction Enhanced Sparse Testing For Multi-Environment Trials, Diego Jarquin, Reka Howard, José Crossa, Yoseph Beyene, Manje Gowda, Johannes W.R. Martini, Giovanny Covarrubias Pazaran, Juan Burgueño, Angela Pacheco, Martin Grondona, Valentin Wimmer, Boddupalli M. Prasanna

Department of Agronomy and Horticulture: Faculty Publications

“Sparse testing” refers to reduced multi-environment breeding trials in which not all genotypes of interest are grown in each environment. Using genomic-enabled prediction and a model embracing genotype · environment interaction (GE), the non-observed genotype-in-environment combinations can be predicted. Consequently, the overall costs can be reduced and the testing capacities can be increased. The accuracy of predicting the unobserved data depends on different factors including (1) how many genotypes overlap between environments, (2) in how many environments each genotype is grown, and (3) which prediction method is used. In this research, we studied the predictive ability obtained when using a …

Effects Of Tank Contamination And Impact Of Drift-Reducing Agents On Weed Control In Response To Dicamba Applications, Milos Zaric

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Availability of dicamba-tolerant (DT) crops from 2017 provided farmers with additional herbicides for weed control management in row crops. However, the technology alike this one has concerns regarding dicamba off-target movement (OTM) causing undesirable effects on sensitive vegetation. Even though dicamba has high water solubility OTM that has often been overlooked when it comes to unintended crop exposure is dicamba tank contamination. Considering the complexity of spraying equipment soybean response may be expected even when small amounts of residues are left in the spray equipment. Typically, the same field spray equipment is used to perform herbicide application through growing season …

Irrigation, Carbon Amelioration, Nitrogen, And Stover Removal Effects On Continuous Corn, Marty Schmer, Virginia L. Jin, Richard B. Ferguson, Brian J. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Corn (Zea mays L.) residue or stover is harvested as supplemental feed for livestock and is a potential feedstock for cellulosic biofuels. Limited information is available on corn stover removal effects on grain yield under different irrigation rates, nitrogen (N) fertilizer rates, and practices to maintain soil carbon (C) and minimize soil erosion. We evaluated potential interactions between irrigation rate (full or limited), C amelioration practices (cover crop, surface-applied manure, or no amelioration practice), fertilizer N management (125 or 200 kg N ha⁻¹), and stover removal (residue removal or no residue removal), on no-till continuous corn …

Weed Control Efficacy Of Bio-Based Sprayable Mulch Films In Specialty Crop Systems, Eliott Gloeb

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

A sprayable bio-based mulch film (biofilm) could present a more sustainable weed management tool for specialty crop producers than conventional plastic mulch films while also offering flexibility in application patterns and timing. From 2017 to 2019, six greenhouse trials and four field trials were conducted at the University of Nebraska-Lincoln to study the effects of biofilm application on weed suppression and crop yields. Multiple application rates (0.81 L/m² - 9.78 L/m²) and application times (prior to weed emergence and post weed emergence) were tested. Corn starch, glycerol, keratin hydrolysate, corn gluten meal, corn zein, and isolated soy …

Characterizing Variation In Nitrogen Use Efficiency In Wheat Genotypes Using Proximal Canopy Sensing For Sustainable Wheat Production, Mohammed A. Naser, Raj Khosla, Louis Longchamps, Subash Dahal

Department of Agronomy and Horticulture: Faculty Publications

Global nitrogen use efficiency (NUE) for cereal production is marginal and is estimated to be about 33%. Remote sensing tools have tremendous potential for improving NUE in crops through efficient nitrogen management as well as the identification of high-NUE genotypes. The objectives of this study were (i) to identify and quantify the variation in NUE across 24 winter wheat genotypes (Triticum aestivum L.) and (ii) to determine if the normalized difference vegetation index (NDVI) could characterize the variability in NUE across wheat genotypes. This study was conducted in 2010 and 2011 in the semi-arid climate of Northeastern Colorado across dryland …

Genome Wide Association Study Reveals Plant Loci Controlling Heritability Of The Rhizosphere Microbiome, Siwen Deng, Daniel F. Caddell, Gen Xu, Lindsay Dahlen, Lorenzo Washington, Jinliang Yang, Devin Coleman-Derr

Department of Agronomy and Horticulture: Faculty Publications

Host genetics has recently been shown to be a driver of plant microbiome composition. However, identifying the underlying genetic loci controlling microbial selection remains challenging. Genome-wide association studies (GWAS) represent a potentially powerful, unbiased method to identify microbes sensitive to the host genotype and to connect them with the genetic loci that influence their colonization. Here, we conducted a population-level microbiome analysis of the rhizospheres of 200 sorghum genotypes. Using 16S rRNA amplicon sequencing, we identify rhizosphere-associated bacteria exhibiting heritable associations with plant genotype, and identify significant overlap between these lineages and heritable taxa recently identified in maize. Furthermore, we …

Assessing Approaches For Stratifying Producer Fields Based On Biophysical Attributes For Regional Yield-Gap Analysis, Spyridon Mourtzinis, Patricio Grassini, Juan I. Rattalino Edreira, José F. Andrade, Peter M. Kyveryga, Shawn P. Conley

Department of Agronomy and Horticulture: Faculty Publications

Large databases containing producer field-level yield and management records can be used to identify causes of yield gaps. A relevant question is how to account for the diverse biophysical background (i.e., climate and soil) across fields and years, which can confound the effect of a given management practice on yield. Here we evaluated two approaches to group producer fields based on biophysical attributes: (i) a technology extrapolation domain spatial framework (‘TEDs’) that delineates regions with similar (long-term average) annual weather and soil water storage capacity and (ii) clusters based on field-specific soil properties and weather during each crop …

Midseason Nitrogen Sufficiency Guidelines For Corn Production In Arkansas Based On Tissue Analysis And Remote Sensing, Caio Luiz Dos Santos

Graduate Theses and Dissertations

There are a lack of tools to assess midseason nitrogen (N) status in corn (Zea mays L.) production systems and identify the need for additional N fertilization. This study was conducted to determine the ability of leaf N concentration (LN) and the Dark Green Color Index (DGCI) at the 10th collared leaf stage (V10), the 12th collared leaf stage (V12), and tasseling (VT) to predict if midseason N is required to maximize corn grain yield. From 2017 to 2019, eight field studies with 11 N treatments ranging from 0 to 258 kg N ha-1 were conducted on silt loam soils …

Resilience To Large, “Catastrophic” Wildfires In North America's Grassland Biome, Victoria M. Donovan, Dirac Twidwell, Daniel R. Uden, Tsegaye Tadesse, Brian D. Wardlow, Christine H. Bielski, Matthew O. Jones, Brady W. Allred, David E. Naugle, Craig R. Allen

Department of Agronomy and Horticulture: Faculty Publications

Wildfires are ecosystem‐level drivers of structure and function in many vegetated biomes. While numerous studies have emphasized the benefits of fire to ecosystems, large wildfires have also been associated with the loss of ecosystem services and shifts in vegetation abundance. The size and number of wildfires are increasing across a number of regions, and yet the outcomes of large wildfire on vegetation at large‐scales are still largely unknown. We introduce an exhaustive analysis of wildfire‐scale vegetation response to large wildfires across North America's grassland biome. We use 18 years of a newly released vegetation data set combined with 1,390 geospatial …

Control Of Volunteer Corn In Enlist Corn And Economics Of Herbicide Programs For Weed Control In Conventional And Multiple Herbicide-Resistant Soybean Across Nebraska, Adam Michael Striegel

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

With commercialization of multiple herbicide-resistant corn and soybean cultivars, producers have new management options for controlling herbicide-resistant weeds and volunteer corn. Corn-on-corn production systems are common in irrigated fields in southcentral Nebraska which can create issues with volunteer corn management in corn fields. Enlist corn contains a new multiple herbicide-resistant trait providing resistance to 2,4-D choline, glyphosate, and the aryloxyphenoxypropionate (FOPs). Field experiments were conducted in 2018 and 2019 at South Central Agricultural Laboratory near Clay Center, Nebraska with the objective to evaluate ACCase-inhibiting herbicides and herbicide application timing on volunteer corn control, Enlist corn injury, and yield. Glyphosate/glufosinate-resistant corn …

Buffalograss Diversity Assessment, Jesse Collin Marshall

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Buffalograss [Buchloë dactylodes (Nutt.) Engelm. syn. Bouteloua dactyloides (Nutt.) Columbus] is a low input alternative turfgrass for the Great Plains region. Buffalograss is a dioecious stoloniferous warm season grass with wide variation of valuable traits and is well adapted for use in minimal maintenance landscapes. The obligate outcrossing nature of the species and wide variation of potentially valuable traits make buffalograss a prime candidate for cultivar improvement and genetic study. Limited information is available regarding buffalograss genetics. In effort to expand genetic resources around buffalograss, we developed buffalograss derived simple sequence repeat markers from previously available transcriptomes of buffalograss …

Reaction Of Tepary Beans To Eight Virulent Races Of The Rust Pathogen That Overcomes All Known Common Bean Rust Resistance Genes, Santos Barrera Lemus, Prabin Tamang, Carlos A. Urrea, Marcial A. Pastor-Corrales

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Bean rust, caused by Uromyces appendiculatus, is a major disease of common and snap bean (Phaseolus vulgaris) worldwide (Stavely, 1984). Although host resistance is an important component of rust management (Mmbaga et al., 1996), populations of the rust pathogen comprise an extensive and shifting virulence diversity that could render susceptible all known rust resistance genes in common bean. Conversely, it has been suggested that certain tepary bean (Phaseolus acutifolius) accessions are broadly resistant to bean rust (Miklas & Stavely, 1998). The objectives of this study were to verify if tepary beans are resistant to …

Extreme Fire As A Management Tool To Combat Regime Shifts In The Range Of The Endangered American Burying Beetle, Alison K. Ludwig, Daniel R. Uden, Dirac Twidwell

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

This study is focused on the population of federally-endangered American burying beetles in south-central Nebraska. It is focused on changes in land cover over time and at several levels of spatial scale, and how management efforts are impacting both the beetle and a changing landscape. Our findings are applicable to a large portion of the Great Plains, which is undergoing the same shift from grassland to woodland, and to areas where the beetle is still found.

Variance Heterogeneity Genome-Wide Mapping For Cadmium In Bread Wheat Reveals Novel Genomic Loci And Epistatic Interactions, Waseem Hussain, Malachy T. Campbell, Diego Jarquin, Harkamal Walia, Gota Morota

Department of Agronomy and Horticulture: Faculty Publications

Genome-wide association mapping identifies quantitative trait loci (QTL) that influence the mean differences between the marker genotypes for a given trait. While most loci influence the mean value of a trait, certain loci, known as variance heterogeneity QTL (vQTL) determine the variability of the trait instead of the mean trait value (mQTL). In the present study, we performed a variance heterogeneity genome-wide association study (vGWAS) for grain cadmium (Cd) concentration in bread wheat. We used double generalized linear model and hierarchical generalized linear model to identify vQTL associated with grain Cd. We identified novel vQTL regions on chromosomes 2A and …

A Global Perspective On Sustainable Intensification Research, Kenneth Cassman, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Despite general agreement that meeting food demand without further loss of natural ecosystems requires sustainable intensification, there is little dialogue about the research agenda needed to achieve it. To that end, we evaluate current trajectories towards sustainable intensification, review published research on the topic, identify missing links, and propose a prioritization framework to fill gaps. Although progress towards sustainable intensification is behind schedule, we are optimistic that current trends can get back on course assuming a well-prioritized and adequately funded research portfolio and appropriate policies and institutions to support it.

Herbicide Injury From Dicamba And 2,4-D: How Much Is Too Much In Lettuce?, Xinzheng Chen, Amit J. Jhala, Stevan Z. Knezevic, Samuel E. Wortman

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Off-target herbicide injury from dicamba and 2,4-D is an increasingly common problem for specialty crop growers in the Midwest United States. Lettuce (Lactuca sativa L.) is a common specialty crops grown in Nebraska, but proximity to corn and soybean production leaves growers vulnerable to crop injury and significant economic loss. The goal of this study was to quantify crop injury and yield loss in greenhouse grown lettuce after exposure to simulated sub-lethal drift rates of 2,4-D and dicamba. Sublethal doses were determined based on a percentage of the maximum labeled rate and ranged from 25% to 0.01%. Tested lettuce …

Spring Meadow Management Practices: What’S A Rancher To Do?, Tara M. Harms, Jerry D. Volesky, Mitchell B. Stephenson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Subirrigated meadows are a valuable forage resource to ranching operations in the Nebraska Sandhills, being used for both hay production and livestock grazing.

The water table of these meadows is within one meter of the soil surface during the growing season.

In some years, wet conditions hinder meadow utilization, resulting in a buildup of standing dead and litter plant material which can lower forage production.

Investigate if burning and mowing are effective strategies to remove dead plant material from meadows • Determine if burning or mowing interact with grazing to influence end of season forage production

Burning or mowing effectively …

Optimum Rates Of Surface-Applied Coal Char Decreased Soil Ammonia Volatilization Loss, Dinesh Panday, Maysoon M. Mikha, Harold P. Collins, Virginia L. Jin, Michael Kaiser, Jennifer Cooper, Arindam Malakar, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Fertilizer N losses from agricultural systems have economic and environmental implications. Soil amendment with high C materials, such as coal char, may mitigate N losses. Char, a coal combustion residue, obtained from a sugar factory in Scottsbluff, NE, contained 29% C by weight. A 30-d laboratory study was conducted to evaluate the effects of char addition on N losses via nitrous oxide (N₂O) emission, ammonia (NH₃) volatilization, and nitrate (NO₃–N) leaching from fertilized loam and sandy loam soils. Char was applied at five different rates (0, 6.7, 10.1, 13.4, and 26.8 Mg C ha …

Using Ndvi To Differentiate Wheat Genotypes Productivity Under Dryland And Irrigated Conditions, Mohammed A. Naser, Raj Khosla, Louis Longchamps, Subash Dahal

Department of Agronomy and Horticulture: Faculty Publications

Crop breeders are looking for tools to facilitate the screening of genotypes in field trials. Remote sensing-based indices such as normalized difference vegetative index (NDVI) are sensitive to biomass and nitrogen (N) variability in crop canopies. The objectives of this study were (i) to determine if proximal sensor-based NDVI readings can differentiate the yield of winter wheat (Triticum aestivum L.) genotypes and (ii) to determine if NDVI readings can be used to classify wheat genotypes into grain yield productivity classes. This study was conducted in northeastern Colorado in 2010 and 2011. The NDVI readings were acquired weekly from March to …