Agronomy and Crop Sciences Commons^™

Exome-Based New Allele-Specific Pcr Markers And Transferability For Sodicity Tolerance In Bread Wheat (Triticum Aestivum L.), Roopali Bhoite, Rosemary Smith, Urmil Bansal, Mirza Dowla, Harbans Bariana, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Targeted exome-based genotype by sequencing (t-GBS), a sequencing technology that tags SNPs and haplotypes in gene-rich regions was used in previous genome-wide association studies (GWAS) for sodicity tolerance in bread wheat. Thirty-nine novel SNPs including 18 haplotypes for yield and yield-components were identified. The present study aimed at developing SNP-derived markers by precisely locating new SNPs on ~180 bp allelic sequence of t-GBS, marker validation, and SNP functional characterization based on its exonic location. We identified unknown locations of significant SNPs/haplotypes by aligning allelic sequences on to IWGSC RefSeqv1.0 on respective chromosomes. Eighteen out of the target 39 SNP locations …

Targeted Sequencing Of The Panicum Miliaceum Gene Space And Genotyping Of Variant Sites From Population Genetics Studies, Combined In A Single Assay, As A Tool For Broomcorn Millet Assisted Breeding, Gabriele Magris, Serena Foria, Silvano Ciani, Dipak K. Santra, Ombretta Polenghi, Virna Cerne, Michele Morgante, Gabriele Di Gaspero

Department of Agronomy and Horticulture: Faculty Publications

Broomcorn millet (Panicum miliaceum L.) is a prehistorical cereal, today cultivated as a minor crop with low yields but with a renewed interest for its high water use effciency and gluten-free grains. To reverse the downward trend in broomcorn millet cultivation, the crop needs genetic improvement and creation of novel genetic variation to increase productivity. In order to facilitate genomics-assisted breeding, we designed a reduced representation genome-sequencing assay that investigates 1.8% of the nuclear DNA in a targeted and reproducible way, with an intensity of genomic sampling that is a direct function of local recombination rate. We used this …

Indigenous Lakadong Turmeric Of Meghalaya And Its Future Prospects, Manjit Kumar Ray, Dipak K. Santra, Piyush Kumar Mishra, Saurav Das

Department of Agronomy and Horticulture: Faculty Publications

Turmeric (Curcuma longa) has long been used in traditional Indian medicine. India accounts for 80% of total global turmeric production. Lakadong turmeric gets its name from the tiny village of Lakadong, which is located in the foothills of the Jaintia Hills in Meghalaya, India. It is known for having a high curcumin content of more than 7%, as opposed to 2 - 4% in regular varieties. The tribes of this region brought Lakadong turmeric from the forest and domesticated it for medicinal purposes centuries ago. Growth in local coal industries and a gradual decline in the market have …

Effect Of Cutting Management On The Forage Production And Quality Of Tepary Bean (Phaseolus Acutifolius A. Gray), Travis W. Witt, Brian K. Northup, Timothy G. Porch, Santos Barrera, Carlos A. Urrea

Department of Agronomy and Horticulture: Faculty Publications

Tepary bean (Phaseolus acutifolius A. Gray) is an underutilized drought tolerant annual legume, originating from the Sonoran Desert, that may be a beneficial forage/hay for beef cattle in the Southern Great Plains of the US (SGP). The SGP has erratic rainfall and periods of intermittent drought exacerbated by high summer temperatures. In 2020 and 2021, a split-plot design was used to evaluate 13 genotypes of tepary bean and a forage soybean (control) at El Reno, OK, USA to compare production of plant biomass and forage nutritive value parameters under seven harvest regimes. Genotypes were used as the main plot …

Protecting Wa Crops Issue 32 - August 2023, Department Of Primary Industries And Regional Development, Western Australia

Protecting WA Crops

Protecting WA Crops Issue 32 - August 2023 contents:

• Sclerotinia management for narrow leaf lupins crops
• Meet Crop Protection team member – Ciara Beard

Heat Stress-Induced Lipid Alterations In Peanut, Walker Spivey

All Theses

Peanut (Arachis hypogaea) is a globally important crop cultivated for human consumption and for the production of seed oil. As anthropogenic climate change continues to challenge global crop production with extreme environmental conditions such as high temperatures, the development of heat-tolerant varieties is crucial to meet peanut production demands. At the cellular level, one of the fundamental causes of yield loss at high temperatures (HT) is membrane damage. This work reports the investigations on a subset of a peanut recombinant inbred line population demonstrating that the membrane lipid remodeling occurring at HT is consistent with homeoviscous adaptation to …

A Comparison Of Different Stomatal Density Phenotypes Of Hordeum Vulgare Under Varied Watering Regimes Reveals Superior Genotypes With Enhanced Drought Tolerance, Brittany Clare Robertson, Yong Han, Chengdao Li

Grain and Other Field Crops Research Articles

Enhancing the water-use efficiency (WUE) of barley cultivars may safeguard yield deficits during periods of low rainfall. Reduced stomatal density is linked to enhanced WUE, leading to improved drought resistance across plant genera. In this study, 10 barley varieties exhibiting a range of stomatal density phenotypes were grown under differing soil water contents to determine whether stomatal density influences the capacity of genotypes to resist low water availability. The low-stomatal-density genotype Hindmarsh showed the least impact on biomass production during early development, with a 37.13% decrease in dry biomass during drought treatment. Low-stomatal-density genotypes additionally outcompeted high-stomatal-density genotypes under water-deprivation …

Lunchbox Hydroponics, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

Growing plants with hydroponics is less complicated than it may appear when the fundamentals of the system are understood. This publication provides instruction on the construction of a simple hydroponic prototype suitable for school or youth education programs. The fundamentals of hydroponics, basic design of a solution system, seed starting, creating a nutrient solution, and plant care are explored through this engaging project. This prototype can successfully be used to grow small edible and herb plants in the classroom or at home.

Electrochemically Precipitated Struvite Effects On Soil Property And Crop Response, Tatum Simms

Graduate Theses and Dissertations

Phosphorus (P) is a vital resource that is finite and only mineable in raw-material form in a few countries worldwide. Struvite (MgNH4PO4·6H2O) is a mineral that can be produced from wastewater that may provide a promising alternative source of fertilizer-P for crop production, but comprehensive characterizations of struvite behavior in various soils and responses of various crops are necessary. This study aimed to determine the (1) leaching potential, (2) vertical soil profile-P distribution, (3) wheat (Triticum aestivum), corn (Zea mays), and soybean (Glycine max) response, and (4) soil property changes over time of electrochemically precipitated struvite (ECST) compared to that …

Increasing Rye Cover Crop Biomass Production After Corn Residue Removal To Balance Economics And Soil Health, Sabrina J. Ruis, Humberto Blanco-Canqui, Paul J. Jasa, Glen Slater, Richard B. Ferguson

Department of Agronomy and Horticulture: Faculty Publications

Low or variable cover crop (CC) biomass production could limit CC benefits. Longer CC growing periods via late termination could increase CC benefits, especially under limited crop residue return. We studied whether early (2–3 wk before planting)- or late (at planting)-terminated winter rye (Secale cereale L.) CC maintains soil properties, crop yields, and farm income under 0%, 25%, 50%, 75%, and 100% corn (Zea mays L.) residue removal in rainfed and irrigated no-till in the U.S. Great Plains after 6 yr. Early-terminated CCs produced < 1 Mg ha^-1 of biomass while late-terminated CCs averaged 1.6 Mg ha^-1 at the rainfed …

Soybean Tolerance To Ultra-Low Doses Of Dicamba: Hormesis Or Not, Luka Milosevic, O. Adewale Osipitan, Jon E. Scott, Stevan Z. Knezevic

Department of Agronomy and Horticulture: Faculty Publications

The widespread use of dicamba across the soybean growing regions of the United States resulted in increased off-target movement of this herbicide to susceptible vegetations. There are speculations that a drift of sublethal (ultra-low) doses of dicamba can enhance soybean growth and yield through a phenomenon called hormesis. Field studies were conducted during 2018 and 2019 to determine whether soybean growth and yield can be enhanced with ultra-low doses (0.0112–56 g ae ha^-1) of dicamba, applied at three growth stages (V2, R1 and R2). There was no evidence that the ultra-low doses of dicamba (0.0112–56 g ae ha …

Unraveling Prevalence And Effects Of Deleterious Mutations In Maize Elite Lines Across Decades Of Modern Breeding, Shichao Sun, Baobao Wang, Changyu Li, Gen Xu, Jinliang Yang, Matthew B. Hufford, Jeffrey Ross-Ibarra, Haiyang Wang, Li Wang

Department of Agronomy and Horticulture: Faculty Publications

Future breeding is likely to involve the detection and removal of deleterious alleles, which are mutations that negatively affect crop fitness. However, little is known about the prevalence of such mutations and their effects on phenotypic traits in the context of modern crop breeding. To address this, we examined the number and frequency of deleterious mutations in 350 elite maize inbred lines developed over the past few decades in China and the United States. Our findings reveal an accumulation of weakly deleterious mutations and a decrease in strongly deleterious mutations, indicating the dominant effects of genetic drift and purifying selection …

Method Developments To Identify Loci And Selection Patterns Associated With Genotype By Environment Interactions In Soybean, Mary M. Happ

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

For many complex traits such as grain yield, genotype by environment (GxE) interactions are a prevalent source of phenotypic variation. Exploring the capacity of different methodologies to help describe and quantify the GxE interaction landscape for grain yield is an important step in informing plant breeders what the most viable strategies for management and exploitation of GxE may be. In this endeavor, we compared the results from multiple genome wide association studies (GWAS) that used either stability estimators as a phenotype to capture GxE variance, or directly mapped GxE in a mixed model for yield. Leading into this study, a …

Crop-Livestock Diversification And Efficiency In Agriculture, Jay Parsons, Maroua Afi

Cornhusker Economics

Diversification is a familiar strategy for managing risk in agriculture. It can take several forms including growing more than one crop or operating a farm with both crop and livestock enterprises. As with other strategies for managing risk, diversification comes with a unique set of costs. Managing a farm with multiple enterprises creates additional overhead as well as additional demands on management, labor, land, capital, and other resources. Diversification adds complexity to an operation and too much complexity can lead to inefficiencies.

Genomes To Fields 2022 Maize Genotype By Environment Prediction Competition, Dayane Cristina Lima, Jacob D. Washburn, José Ignacio Varela, Qiuyue Chen, Joseph L. Gage, Maria Cinta Romay, James Holland, David Ertl, Marco Lopez-Cruz, Fernando M. Aguate, Gustavo De Los Campos, Shawn Kaeppler, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint-Garcia, Michael A. Gore, Candice N. Hirsch, Joseph E. Knoll, John Mckay, Richard Minyo, Seth C. Murray, Osler A. Ortez, James C. Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Erin E. Sparks, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Teclemariam Weldekidan, Wenwei Xu, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Objectives The Genomes to Fields (G2F) 2022 Maize Genotype by Environment (GxE) Prediction Competition aimed to develop models for predicting grain yield for the 2022 Maize GxE project field trials, leveraging the datasets previously generated by this project and other publicly available data.

Data description This resource used data from the Maize GxE project within the G2F Initiative [1]. The dataset included phenotypic and genotypic data of the hybrids evaluated in 45 locations from 2014 to 2022. Also, soil, weather, environmental covariates data and metadata information for all environments (combination of year and location). Competitors also had access to ReadMe …

Role Of Biotechnology In Creating Sustainable Agriculture, Saurav Das, Manjit Kumar Ray, Dinesh Panday, Piyush Kumar Mishra

Department of Agronomy and Horticulture: Faculty Publications

This narrative review paper discusses the role of biotechnology in the development of sustainable agriculture. The paper begins by defning sustanability and highlights the importance of biotechnology in establishing sustainable agriculture. Sustainable agriculture is an approach that prioritizes meeting current food and fiber production needs while conserving and enhancing natural resources for future generations. To achieve agricultural sustainability, it is necessary to strike a balance between economic viability, environmental stewardship, and social responsibility. This can be difficult, especially in the face of biotic and abiotic stresses such as pests, diseases, climate change, soil degradation, and water depletion. The prevalence of …

Genetic Resources And Precise Gene Editing For Targeted Improvement Of Barley Abiotic Stress Tolerance, Sakura Karunarathne, Esther Walker, Darshan Sharma, Chengdao Li, Yong Han

Grain and Other Field Crops Research Articles

Abiotic stresses, predominately drought, heat, salinity, cold, and waterlogging, adversely affect cereal crops. They limit barley production worldwide and cause huge economic losses. In barley, functional genes under various stresses have been identified over the years and genetic improvement to stress tolerance has taken a new turn with the introduction of modern gene-editing platforms. In particular, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is a robust and versatile tool for precise mutation creation and trait improvement. In this review, we highlight the stress-affected regions and the corresponding economic losses among the main barley producers. We collate about …

Protecting Wa Crops Issue 31 - July 2023, Department Of Primary Industries And Regional Development, Western Australia

Protecting WA Crops

Protecting WA Crops Issue 31 - July 2023 contents:

• New project to reduce Rhizoctonia impact in low and medium rainfall zones
• Meet Crop Protection team member – Dr Ahmed Saad

Evaluating Drill Interseeded Cover Crop Establishment And Nitrogen Impact In Irrigated Corn, Victor De Sousa Ferreira

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The adoption of cover crops as a strategy to improve soil health and cropping systems sustainability is on the rise in the United States. PRE herbicides with soil residual activity are widely applied in corn production systems to prevent early season weed development, crop-weed competition, and yield loss. When preemergence herbicides are applied in the field, the active ingredients remain in the soil rhizosphere for a period of time, killing weed seedlings as they emerge. However, PRE herbicides can also impact the establishment of interseeded cover crops. Greenhouse bioassay was conducted to evaluate the preemergence herbicide carry-over potential to interseeded …

Skeleton Weed Program 2022/2023: Annual Report To Grain Growers, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

The Skeleton Weed Program has been funded by Western Australian (WA) growers through the Grains, Seed, and Hay Industry Funding Scheme (GSHIFS) since 2010. During that time, the program has grown, placing a greater emphasis on working with farmers, local coordination, support, and encompassing critical research and development.

The Skeleton Weed Program continues to provide critical assistance to grain growers in the management of skeleton weed through the:

• searching of more than 400,000 ha of land to map infested areas for treatment.

• chemical control of skeleton weed on 10,000 ha of infested land.

• successful eradication of skeleton …

Study Of Production And Adaptation Characters Of Some Newly Obtained Genotypes Of Durum Wheat (Triticum Durum Desf.) In Sub-Humid Region (El Harrouch North-Eastern Of Algeria), Lamia Bourouh, Nabila Souilah, Boulacel Mouad, Ghania Chaib, Tahar Hazmoune, Bendif Hamdi

Journal of Bioresource Management

The experiment was carried out at DAOUDI Larbi pilot farm in El Harrouch, during the 2021/2022 crop year, under sub-humid weather conditions. The main objective of this research is based on the study of production and adaptation characters of seven durum wheat newly obtained genotypes (Triticum durum Desf.). The results of descriptive sheets according to UPOV recommendations (2014-2017), showed diversity between the varieties studied, such as: plant height, straw section, awns (presence, disposition, anthocyanin pigmentation, color, and length), ear (density, color, shape, length and glaucescence), lower glume (shape, color, weight and shape of shoulder, length and curvature of beak), …

Classim: A Relational Database Driven Crop Model Interface, Dennis Timlin, David Fleisher, Maura Maura, Kirsten Paff, Wenguang Sun, Sahila Beegum, Sanai Li, Zhuangji Wang, Vangimalla Reddy

Department of Agronomy and Horticulture: Faculty Publications

Crop models are valuable tools for examining the interactions of cultivar characteristics, environment, and management practices, and how they affect crop growth and development. The difficulty in finding all the data needed to set up a simulation can often deter potential users from utilizing a crop model. Model interfaces are necessary to make these complex tools accessible to end-users who may lack the expertise needed to work with the models directly, but who would benefit from the information generated by the models. As crop models vary in terms of input and output structures, there is no one universally compatible interface, …

Conversion Of Native Grassland To Coniferous Forests Decreased Stocks Of Soil Organic Carbon And Microbial Biomass, Lidong Li, Elnaz Hosseiniaghdam, Rhae A. Drijber, Elizabeth Jeske, Tala Awada, J. Hiller, Michael Kaiser

Department of Agronomy and Horticulture: Faculty Publications

Aims Encroachment of woody species into grasslands is a global phenomenon that affects ecosystem services, including soil organic carbon (SOC) storage and microbial community structure. We determined stocks of SOC and soil microbial biomass as affected by conversion of grasslands to coniferous forests.

Methods We examined SOC and soil δ¹³C signatures under three vegetation covers: native grasslands, eastern redcedar (Juniperus virginiana), and ponderosa pine (Pinus ponderosa), at six soil depths (0 − 10, 10 − 30, 30 − 100, 100 − 170, 170 − 240, 240 − 300 cm). Fatty acid methyl esters (FAMEs) …

Tillage, Green Manuring And Crop Residue Management Impacts On Crop Productivity, Potassium Use Efficiency And Potassium Fractions Under Rice-Wheat System, Sandeep Sharma, Pritpal Singh, Hayssam M. Ali, Manzer Hussain Siddiqui, Javed Iqbal

Department of Agronomy and Horticulture: Faculty Publications

The conventional crop production practices including intensive tillage and open field crop residue burning in world’ largest rice-wheat system (RWS) are adversely affecting crop productivity besides deteriorating natural resources and ecosystems’ sustainability. In order to improve system productivity, potassium (K) use efficiency and apparent K balance, adoption of conservation tillage in a RWS with residue management is considered highly effective. We therefore, studied the effect of wheat straw retention and green manure (GM) in rice (main plot treatment), and tillage and rice residue management in subsequent wheat (sub-plot treatments) on crop productivity, K use efficiency and its transformation amongst different …

First Things First: Widespread Nutrient Deficiencies Limit Yields In Smallholder Oil Palm Fields, Hendra Sugianto, Juan Pablo Monzon, Iput Pradiko, Fatima A. Tenorio, Ya Li Lim, Christopher R. Donough, Sunawan, Suroso Rahutomo, Fahmuddin Agus, James Cock, Joni Amsar, Rana Farrasati, Ridho Iskandar, Juan I. Rattalino Edreira, Shofia Saleh, Heri Santoso, Antonius P. Tito, Nadib Ulfaria, Maja A. Slingerland, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

CONTEXT: Indonesia is the most important oil palm producing country. Nearly 40% of planted area is managed by smallholders, with yields well below the potential. Efforts to increase productivity have focused on the source of planting material, with little attention paid to plant nutrition.

OBJECTIVE: To assess the degree to which current productivity in smallholder oil palm fields is limited by nutrients in scenarios with distinct sources of planting material.

METHODS: We collected detailed data on leaf nutrient concentration from 30 fields to derive minimum sampling size needed to diagnose nutrient status. Subsequently, we collected data on …

Editorial: New Insights Into Mechanisms Of Epigenetic Modifiers In Plant Growth And Development, Volume Ii, Ming Luo, Tomasz Jacek Sarnowski, Marc Libault, Gabino Ríos, Jean-Benoit Charron, Nitin Mantri, Shoudong Zhang

Department of Agronomy and Horticulture: Faculty Publications

As we have learned, chromatin modifications, including histone modifications and DNA methylation, play a key role in plant development (Ng and Bird, 1999). However, accumulated evidence shows that, besides chromatin biochemical modifications, other epigenetic regulations such as chromatin architecture also function at a pivot point to regulate plant development (Zhang et al., 2021). In this Research Topic, five research papers describe multiple developmental facets mediated by different epigenetic mechanisms besides histone modifications. These manuscripts report experimental evidence or summarize recent advances in epigenetic regulations of some important developmental genes or development-related mechanisms. This Research Topic allows readers to learn of …

A Complete Telomere-To-Telomere Assembly Of The Maize Genome, Jian Chen, Zijian Wang, Kaiwen Tan, Wei Huang, Junpeng Shi, Tong Li, Jiang Hu, Kai Wang, Chao Wang, Beibei Xin, Haiming Zhao, Weibin Song, Matthew B. Hufford, James C. Schnable, Weiwei Jin, China Agricultural University, Hainan Yazhou Bay Seed Laboratory

Department of Agronomy and Horticulture: Faculty Publications

A complete telomere-to-telomere (T2T) finished genome has been the long pursuit of genomic research. Through generating deep coverage ultralong Oxford Nanopore Technology (ONT) and PacBio HiFi reads, we report here a complete genome assembly of maize with each chromosome entirely traversed in a single contig. The 2,178.6 Mb T2T Mo17 genome with a base accuracy of over 99.99% unveiled the structural features of all repetitive regions of the genome. There were several super-long simple-sequence-repeat arrays having consecutive thymine–adenine– guanine (TAG) tri-nucleotide repeats up to 235 kb. The assembly of the entire nucleolar organizer region of the 26.8 Mb array with …

Genome-Wide Association Analysis For Emergence Of Deeply Sown Rice (Oryza Sativa) Reveals Novel Aus-Specific Phytohormone Candidate Genes For Adaptation To Dry-Direct Seeding In The Field, Sandeep A. Sakhale, Shailesh Yadav, Lindsay V. Clark, Alexander E. Lipka, Arvind Kumar, Erik J. Sacks

Department of Agronomy and Horticulture: Faculty Publications

Dry direct-seeded rice (dry-DSR) is typically sown deeply to circumvent the need for irrigation, and thus seedling emergence is a crucial trait affecting plant stand and yield. To breed elite cultivars that use less water and are climate-resilient, an understanding of the genomic regions and underlying genes that confer emergence in deeply sown dry-DSR would be highly advantageous. A combined diversity panel of 470 rice accessions (RDP1 plus aus subset of 3K RGP) was evaluated with 2.9 million single nucleotide polymorphisms (SNPs) to identify associations with dry-DSR traits in the field and component traits in a controlled-environment experiment. Using genome-wide …

Genomic Approaches To Enhance Adaptive Plasticity To Cope With Soil Constraints Amidst Climate Change In Wheat, Roopali Bhoite, Yong Han, Alamuru Krishna Chaitanya, Rajeev K. Varshney, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Climate change is varying the availability of resources, soil physicochemical properties, and rainfall events, which collectively determines soil physical and chemical properties. Soil constraints—acidity (pH < 6), salinity (pH ≤ 8.5), sodicity, and dispersion (pH > 8.5)—are major causes of wheat yield loss in arid and semiarid cropping systems. To cope with changing environments, plants employ adaptive strategies such as phenotypic plasticity, a key multifaceted trait, to promote shifts in phenotypes. Adaptive strategies for constrained soils are complex, determined by key functional traits and genotype × environment × management interactions. The understanding of the molecular basis of stress tolerance is particularly challenging for plasticity traits. Advances in sequencing and high-throughput genomics …

Impact Of Nitrogen Availability On The Accumulation Of Vegetative Lipids In Sorghum, Leticia Felicio Pasqualino

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Sorghum (Sorghum bicolor (L.) Moench) is a versatile and prosperous feedstock crop for renewable energy production. With the increase in bioenergy demand, the production of higher oil content per biomass in a feedstock crop is a highly desirable trait for the biofuel conversion process yield. Especially if this outcome can be achieved using fewer inputs in the field, such as nitrogen. In microalgae species, nitrogen limitation has been associated with changes in the carbon storage metabolic pathway favoring triacylglycerol (TAG) accumulation. In this context, this study aimed to assess whether nitrogen starvation would result in a similar outcome in …