Agronomy and Crop Sciences Commons^™

Structural Variants In The Soybean Genome Localize To Clusters Of Biotic Stress-Response Genes, Leah K. Mchale, William J. Haun, Wayne W. Xu, Pudota B. Bhaskar, Justin E. Anderson, D. L. Hyten, Daniel J. Gerhardt, Jeffrey A. Jeddeloh, Robert M. Stupar

Department of Agronomy and Horticulture: Faculty Publications

Genome-wide structural and gene content variations are hypothesized to drive important phenotypic variation within a species. Structural and gene content variations were assessed among four soybean (Glycine max) genotypes using array hybridization and targeted resequencing. Many chromosomes exhibited relatively low rates of structural variation (SV) among genotypes. However, several regions exhibited both copy number and presence-absence variation, the most prominent found on chromosomes 3, 6, 7, 16, and 18. Interestingly, the regions most enriched for SV were specifically localized to gene-rich regions that harbor clustered multigene families. The most abundant classes of gene families associated with these regions …

Identification Of Positive Yield Qtl Alleles From Exotic Soybean Germplasm In Two Backcross Populations, K.-S. Kim, B. W. Diers, D. L. Hyten, M. A. Rouf Mian, J. G. Shannon, R. L. Nelson

Department of Agronomy and Horticulture: Faculty Publications

Increasing seed yield is an important breeding goal of soybean [Glycine max (L.) Merr.] improvement efforts. Due to the small number of ancestors and subsequent breeding and selection, the genetic base of current soybean cultivars in North America is narrow. The objective of this study was to map quantitative trait loci (QTL) in two backcross populations developed using soybean plant introductions as donor parents. The first population included 116 BC₂F₃-derived lines developed using ‘‘Elgin’’ as the recurrent parent and PI 436684 as the donor parent (E population). The second population included 93 BC₃F …

Molecular Mapping Of Soybean Rust Resistance In Soybean Accession Pi 561356 And Snp Haplotype Analysis Of The Rpp1 Region In Diverse Germplasm, Ki-Seung Kim, Jair R. Unfried, D. L. Hyten, Reid D. Frederick, Glen L. Hartman, Randall L. Nelson, Qijian Song, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean rust (SBR), caused by Phakopsora pachyrhizi Sydow, is one of the most economically important and destructive diseases of soybean [Glycine max (L.) Merr.] and the discovery of novel SBR resistance genes is needed because of virulence diversity in the pathogen. The objectives of this research were to map SBR resistance in plant introduction (PI) 561356 and to identify single nucleotide polymorphism (SNP) haplotypes within the region on soybean chromosome 18 where the SBR resistance gene Rpp1 maps. One-hundred F_2:3 lines derived from a cross between PI 561356 and the susceptible experimental line LD02-4485 were genotyped with genetic …

Advances In Genome Sequencing And Genotyping Technology For Soybean Diversity Analysis, David L. Hyten

Department of Agronomy and Horticulture: Faculty Publications

The completion of a soybean draft whole genome sequence along with advances in sequencing and genotyping technologies are creating a revolution in soybean genomics. The draft whole genome sequence of soybean is allowing researchers to fully take advantage of this new technology and is rapidly changing how soybean germ plasm is mined. Genetic markers such as SNPs can be quickly identified by using next generation sequencing and assayed on a large number of materials using advanced technologies that can genotype tens of thousands of markers on thousands of individuals very rapidly. This ability to qUickly identify and genotype genetic diversity …

High-Resolution Mapping Of Open Chromatin In The Rice Genome, Wenli Zhang, Yufeng Wu, James C. Schnable, Zixian Zeng, Michael Freeling, Gregory E. Crawford, Jiming Jiang

Department of Agronomy and Horticulture: Faculty Publications

Gene expression is controlled by the complex interaction of transcription factors binding to promoters and other regulatory DNA elements. One common characteristic of the genomic regions associated with regulatory proteins is a pronounced sensitivity to DNase I digestion. We generated genome-wide high-resolution maps of DNase I hypersensitive (DH) sites from both seedling and callus tissues of rice (Oryza sativa). Approximately 25% of the DH sites from both tissues were found in putative promoters, indicating that the vast majority of the gene regulatory elements in rice are not located in promoter regions. We found 58% more DH sites in …

Expertos Analizan Impacto Del Cambio Climático En Granos Básicos Y Sorgos Forrajeros En El Salvador, Kimberly Christiansen

INTSORMIL Impacts and Bulletins

En el evento participan profesionales de El Salvador, así como de las universidades de Kansas State y Purdue, de Estados Unidos.

Exponer y analizar los principales efectos derivados del cambio climático en los cultivos de El Salvador, así como el impacto que ha tenido la adopción de variedades forrajeras de sorgo en el país, son los objetivos principales de los foros realizados este miércoles por el Centro Nacional de Tecnología Agropecuaria y Forestal “Enrique Álvarez Córdova” (CENTA) con apoyo del Instituto Internacional de Sorgo, Mijo y otros granos (INTSORMIL).

Arkansas Soybean Performance Tests 2011, R. D. Bond, D. G. Dombek, J. A. Still, R. M. Pryor

Arkansas Agricultural Experiment Station Research Series

Soybean variety and strain performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture Arkansas Crop Variety Improvement Program. The tests provide information to companies developing varieties and/or marketing seed within the state, and aid the Arkansas Cooperative Extension Service in formulating variety recommendations for soybean producers.

Rice (Oryza Sativa) Response To Low Glyphosate Rates As Influenced By Cultivar, Growth Stage, And Imazethapyr Applications, Jason R. Meier

Graduate Theses and Dissertations

Off-target movement of glyphosate onto rice is a perennial concern when rice is grown in close proximity to glyphosate-tolernat crops. If differential tolerance to sub-lethal rates of glyphosate exists among rice (Oryza sativa) cultivars, these cultivars could be utilized in breeding programs or glyphosate-drift sensitive areas. Field and greenhouse experiments were conducted in 2006 and 2007 to examine differences among rice cultivars in response to sub-lethal rates of glyphosate, and to examine imidazolinone-tolerant rice response to imazethapyr and sub-lethal rates of glyphosate applied sequentially to determine the potential for either herbicide to predispose rice to greater injury. In the field …

Arkansas Corn And Grain Sorghum Perfomance Tests 2011, R. D. Bond, D. G. Dombeck, J. A. Still

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

Heritable Epigenetic Variation Among Maize Inbreds, Steve R. Eichten, Ruth A. Swanson, James C. Schnable, Amanda J. Waters, Peter J. Hermanson, Sanzhen Liu, Cheng-Ting Yeh, Yi Jia, Karla Gendler, Michael Freeling, Patrick S. Schnable, Matthew W. Vaughn, Nathan M. Springer

Department of Agronomy and Horticulture: Faculty Publications

Epigenetic variation describes heritable differences that are not attributable to changes in DNA sequence. There is the potential for pure epigenetic variation that occurs in the absence of any genetic change or for more complex situations that involve both genetic and epigenetic differences. Methylation of cytosine residues provides one mechanism for the inheritance of epigenetic information. A genome-wide profiling of DNA methylation in two different genotypes of Zea mays (ssp. mays), an organism with a complex genome of interspersed genes and repetitive elements, allowed the identification and characterization of examples of natural epigenetic variation. The distribution of DNA methylation …

From The Dean And Director, Gary Pierzynski

INTSORMIL Impacts and Bulletins

This issue of the Ag Report has an international theme. It features our efforts to train the Kansas National Guard agribusiness development teams deploying to Afghanistan and some of our international activities in Africa.

Grain Science Honors Alumni And Industry Leaders, Gloria Holcombe

INTSORMIL Impacts and Bulletins

The Department of Grain Science and Industry recognized eight distinguished individuals at its spring luncheon.

Outstanding Service Awards were presented to Aaron Harries, John Khoury,John Mathew, and Jim Rempe. Outstanding Alumni Awards went to Fred Merrill, Bill Pursley, Dale Rodman, and Lloyd Rooney.

Borlaug Fellowship Opens Doors For Rachel Opole [Student Accolades], Mary Lou Peter

INTSORMIL Impacts and Bulletins

Rachel Opole grew up in Nairobi, Kenya’s capitol, and the K-State doctoral student developed an early interest in livestock and crop production. Those interests, largely influenced by her parents, inspired her desire to improve crops grown to feed people. To enhance her work in that area, Opole earned the Norman E. Borlaug Leadership Enhancement in Agriculture Program (LEAP) fellowship, administered by the University of California–Davis.

Beneficial Partnerships: K-Staters Conduct Projects Throughout Africa, Gloria Holcombe, Timothy Dalton

INTSORMIL Impacts and Bulletins

Africa grows many of the same crops as Kansas, including sorghum and wheat. In fact, sorghum germplasm came from Africa, and now Kansas is the No. 1 sorghum producer in the United States.

K-State has expertise in grain storage issues, wheat and grain sorghum milling, and growing crops under dryland and irrigated conditions — vital information for African producers. Faculty secure grant funding to cover travel and other expenses associated with these partnerships. As an added bonus, they bring international experience into the classroom to share with their students. International collaborations help develop networks to share research, establish new markets, …

Plant Pathologist And Grain Scientist Earn K-State’S Highest Honor, Gloria Holcombe

INTSORMIL Impacts and Bulletins

Two innovative College of Agriculture faculty — John Leslie, professor and head of the Department of Plant Pathology, and Xiuzhi “Susan” Sun, professor of grain science and industry — were selected university distinguished professors, a lifetime title and the highest honor K-State bestows on its faculty.

Phenological Development In Two-Row Spring Barley When Grown In A Long-Day (Alberta, Canada) And A Short-Day (Western Australia, Australia) Environment, Blakely Paynter, P E. Juskiw, J H. Helm

Grain and Other Field Crops Research Articles

Phenological development in eight cultivars of two-row, spring barley (Hordeum vulgare L.) was examined when sown at two dates of seeding in two diverse environments. These environments were a short-day environment at Northam, Western Australia, Australia, in 1997 and a long-day environment at Lacombe, Alberta, Canada, in 1998. The Australian and Canadian barley cultivars used had different combinations of basic vegetative phase and daylength sensitivity. Barley grown at Lacombe reached each stage of phenological development in less time than at Northam. Most noticeable was a shorter duration of the period between seedling emergence to double ridge and between double ridge …

Weed Science Research Summaries 2010, K. L. Smith, J A. Bullington, R. C. Doherty, J. R. Meier

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Arkansas Wheat Cultivar Performance Tests 2010-2011, R. E. Mason, R. G. Miller, R. D. Bond, E. A. Milus, J. P. Kelley, R. K. Bacon

Arkansas Agricultural Experiment Station Research Series

Wheat cultivar performance tests are conducted each year in Arkansas by the Arkansas Agricultural Experiment Station, Department of Crop, Soil and Environmental Sciences. The tests provide information to companies developing cultivars and/or marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating cultivar recommendations for small-grain producers.

Miscanthus 3 Giganteus Productivity: The Effects Of Management In Different Environments, Matt Maughan, German Bollero, D.K. Lee, Robert Darmody, Stacy Bonos, Laura Cortese, James Murphy, Roch E. Gaussoin, Matthew Sousek, David Williams, Linda Williams, Fernando Miguez, Thomas Voigt

Department of Agronomy and Horticulture: Faculty Publications

Miscanthus 9 giganteus is a C4 perennial grass that shows great potential as a high-yielding biomass crop. Scant research has been published that reports M. 9 giganteus growth and biomass yields in different environments in the United States. This study investigated the establishment success, plant growth, and dry biomass yield of M. 9 giganteus during its first three seasons at four locations (Urbana, IL; Lexington, KY; Mead, NE; Adelphia, NJ) in the United States. Three nitrogen rates (0, 60, and 120 kg ha -1) were applied at each location each year. Good survival of M. 9 giganteus during its first …

Effects Of In Situ Freezing On Soil Net Nitrogen Mineralization And Net Nitrification In Fertilized Grassland Of Northern China, X. Zhang, W. Bai, Frank S. Gilliam, Q. Wang, X. Han, L. Li

Biological Sciences Faculty Research

Effects of soil freezing on nitrogen (N) mineralization have been the subject of increased attention in the ecological literature, though fewer studies have examined N mineralization responses to successive mild freezing, severe freezing and cyclic freeze–thaw events. Even less is known about relationships of responses to soil N status. This study measured soil N mineralization and nitrification in the field along an experimental N gradient in a grassland of northern China during the dormant season (October 2005–April 2006), a period in which freezing naturally occurs. Net N mineralization exhibited great temporal variability, with nitrification being the predominant N transformation process. …

Gata-Family Transcription Factors In Magnaporthe Oryzae, Cristian F. Quispe

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The filamentous fungus, Magnaporthe oryzae, responsible for blast rice disease, destroys around 10-30% of the rice crop annually. Infection begins when the specialized infection structure, the appressorium, generates enormous internal turgor pressure through the accumulation of glycerol. This turgor acts on a penetration peg emerging at the base of the cell, causing it to breach the leaf surface allowing its infection.

The enzyme trehalose-6- phosphate synthase (Tps1) is a central regulator of the transition from appressorium development to infectious hyphal growth. In the first chapter we show that initiation of rice blast disease requires a regulatory mechanism involving an …

B. R. Wells Rice Research Studies 2010, R. J. Norman, K. A. K. Moldenhauer

Arkansas Agricultural Experiment Station Research Series

The 2010 Rice Research Verification Program ( RRVP) was conducted on twenty-two commercial rice fields across the state. Counties rarticirating in the program included Arkansas, Ashley, Chicot, Clark, Clay, Cross, Desha, Drew, Greene, Jackson, Jefferson, Lafayette, Lawrence, Lonoke, Mississippi, Phillips, Poinsett (2 fields), Prairie, Randolph, St. Francis, and White for a total of 1456 acres. Grain yield in the 2010 RRVP averaged 167 bu/acre ranging from 113 to 215 bu/acre. The 2010 RRVP average yield was 25 bu/acre greater than the estimated Arkansas state average of 142 bu/acre. The highest yielding field was in Clay County with a grain yield …

Herbicide-Resistant Risk Assessment: Response Of Common Nebraska Weeds To Dicamba Dose, Roberto J. Crespo

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Dicamba-resistant soybeans are being developed to provide an additional herbicide mechanism of action that can be used in soybean, and to provide a tool to help manage or mitigate the evolution of other herbicide-resistant weed populations. The objectives of this thesis were to assess the risk of common Nebraska weeds developing resistance to dicamba, quantify baseline dose-response to dicamba of high-risk weed species, and survey the variability in dicamba dose-response among populations of those species. Twenty-five weed scientists were asked to estimate the risk likelihood of ten weed species evolving resistance to dicamba following the commercialization of dicamba-resistant soybean. Palmer …

Sweet Sorghum As An Alternative Cellulosic Biofuel In Eastern Kentucky, Sarah A. Hazenfield

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Sarah A. Hazenfield in June of 2011.

Soil Test And Phosphorus Rate For High Rainfall Clover Pastures, Robert Summers, David Weaver

Bulletins 4000 -

Essential information for soil testing to determine the phosphorus fertiliser requirements for clover pastures in high rainfall coastal areas of Western Australia. Includes procedures for soil sampling, tissue testing, liming, when to apply fertiliser, how to decide on the target production level, how much fertiliser is required and type of fertiliser.

Grinders Convert Sorghum Into Flour, Intsormil

INTSORMIL Impacts and Bulletins

To help contend rising wheat costs Central American bakers are looking for alternative ways to produce their baked products at a low cost without sacrificing nutritional value or taste. Sorghum flour is the answer to their problems. Sorghum flour is about half the cost of wheat flour so using sorghum flour helps bakers save money and make more of a profit. Particle size of sorghum flour must be sufficiently fine in order to substitute for wheat flour in baking recipes. However, small grinders adequate for family level bakers have not been available in Central America. That is until a collaborative …

Uso De Molinos Cti Para La Produccion Y Uso De Harina De Sorgo, Intsormil

INTSORMIL Impacts and Bulletins

Para ayudar a lidiar con el aumento de los costos de trigo en Centro América, los panaderos están buscando formas alternativas para producir sus productos de panadería a un bajo costo sin sacrificar el valor nutritivo o sabor. La harina de sorgo es la respuesta a sus problemas. La harina de sorgo es de aproximadamente la mitad del costo de la harina de trigo, por lo que usar harina de sorgo puede ayudar a los panaderos a ahorrar dinero y disminuir costos de produccion. El tamaño de partícula de la harina de sorgo debe ser lo suficientemente fino como para …

Economic Impacts Of Drought On Kentucky Corn, Hay, And Soybeans, Kortney E. Craft

Masters Theses & Specialist Projects

Understanding climatic impacts is important if we are to comprehend the relationship between climate and society. Weather phenomena can have environmental, economical, and social impacts. Drought is the natural hazard that affects people the most. It is also the most complex and least understood. There is no one universally accepted definition for drought which makes its examination difficult. Droughts’ duration is also difficult to determine because it has no clearly defined onset and end. Also, drought varies both geographically and temporally making uniform drought monitoring difficult. Since drought is difficult to monitor and access, drought impacts are often poorly documented. …

Summaries Of Arkansas Cotton Research 2010, Derrick M. Oosterhuis

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Screening Synteny Blocks In Pairwise Genome Comparisons Through Integer Programming, Haibao Tang, Eric Lyons, Brent S. Pedersen, James C. Schnable, Andrew H. Paterson, Michael Freeling

Department of Agronomy and Horticulture: Faculty Publications

Background:

It is difficult to accurately interpret chromosomal correspondences such as true orthology and paralogy due to significant divergence of genomes from a common ancestor. Analyses are particularly problematic among lineages that have repeatedly experienced whole genome duplication (WGD) events. To compare multiple “subgenomes” derived from genome duplications, we need to relax the traditional requirements of “one-to-one” syntenic matchings of genomic regions in order to reflect “one-to-many” or more generally “many-to-many” matchings. However this relaxation may result in the identification of synteny blocks that are derived from ancient shared WGDs that are not of interest. For many downstream analyses, we …