Agricultural Science Commons^™

Ethylene Production, Cluster Root Formation, And Localization Of Iron(Iii) Reducing Capacity In Fe Deficient Squash Roots, Brian M. Waters, Dale G. Blevins

Department of Agronomy and Horticulture: Faculty Publications

Dicots and non-graminaceous monocots have the ability to increase root iron(III) reducing capacity in response to iron (Fe) deficiency stress. In squash (Cucurbita pepo L.) seedlings, Fe(III) reducing capacity was quantified during early vegetative growth. When plants were grown in Fe-free solution, the Fe(III) reducing capacity was greatly elevated, reached peak activity on day 4, then declined through day 6. Root ethylene production exhibited a temporal pattern that closely matched that of Fe(III) reducing capacity through day 6. On the 7th day of Fe deficiency, cluster root morphology developed, which coincided with a sharp increase in the root Fe(III) …

Variation In Boll Weevil (Coleoptera: Curculionidae) Captures In Pheromone Traps Arising From Wind Speed Moderation By Brush Lines, Thomas W. Sappington, Dale W. Spurgeon

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Paired trap lines of Þve boll weevil pheromone traps each were placed on opposite sides of a brush line at six different sites on a plantation in the Lower Rio Grande Valley of Texas. Temperature, wind speed, and wind direction were monitored with a nearby weather station. We observed a strong negative relationship between mean daily wind speed and total daily capture of boll weevils. About half of the day-to-day variation in weevil captures was explained by wind speed alone. In addition, our data indicate that much of the variation between traps within days may arise from differences in local …

Analysis Of Human Skin Emanations By Gas Chromatography/Mass Spectrometry. 2. Identification Of Volatile Compounds That Are Candidate Attractants For The Yellow Fever Mosquito (Aedes Aegypti), Ulrich R. Bernier, Daniel L. Kline, Donald Barnard, Carl Schreck, Richard A. Yost

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Volatile compounds emanated from human skin were studied by gas chromatography/mass spectrometry (GC/ MS). The purpose of this study was to identify compounds that may be human-produced kairomones which are used for host location by the mosquito, Aedes aegypti (L.). The procedure used to collect volatiles was chosen because of prior knowledge that attractive substances can be transferred from skin to glass by handling. Laboratory bioassays have shown that the residuum on the glass remains attractive to mosquitoes until the compounds of importance evaporate. The sampling and analytical procedures modeled the above-cited process as closely as possible except that the …

Oxygen Metabolism In Plantlbacteria Interactions: Characterization Of The Oxygen Uptake Response Of Plant Suspension Cells, C. Jacyn Baker, E. W. Orlandi, Kenneth L. Deahl

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In recent years the accumulation of reactive oxygen species (ROS) has been studied in plant cell suspension systems treated with bacterial pathogens. However, the associated utilization of molecular oxygen has not been well characterized. Using a multi-electrode oxygen analyser, the rates of oxygen consumption by tobacco cells during bacterial interactions were monitored. Heat-killed (HK) bacteria, which initiate an immediate ROS response in plant cells, were used as an elicitor to avoid complications of oxygen consumption by viable bacteria. An increase in oxygen uptake by the tobacco cells occurred within 4 min after addition of HK-bacteria and lasted for about 10 …

Inhibition Of Cysteine And Aspartyl Proteinases In The Alfalfa Weevil Midgut With Biochemical And Plant-Derived Proteinase Inhibitors, Stephen E. Wilhite, Thomas C. Elden, Joze Brzin, Ann C. Smigocki

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Proteolytic activities in alfalfa weevil (Hypera postica) larval midguts have been characterized. Effects of pH, thiol activators, low-molecular weight inhibitors, and proteinase inhibitors (PIs) on general substrate hydrolysis by midgut extracts were determined. Hemoglobinolytic activity was highest in the acidic to mildly acidic pH range, but was maximal at pH 3.5. Addition of thiolactivators dithiothreitol (DTT), 2-mercaptoethanol (2-ME), or l-cysteine had little effect on hemoglobin hydrolysis at pH 3.5, but enhanced azocaseinolytic activity two to three-fold at pH 5.0. The broad cysteine PI E-64 reduced azocaseinolytic activity by 64% or 42% at pH 5 in the presence or …

Bovine Blastocyst-Derived Trophectoderm And Endoderm Cell Cultures: Interferon Tau And Transferrin Expression As Respective In Vitro Markers, Neil C. Talbot, Thomas J. Caperna, J. Lannett Edwards, Wes Garrett, Kevin D. Wells, Alan D. Ealy

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Continuous cultures of bovine trophectoderm (CT-1 and CT- 5) and bovine endoderm (CE-1 and CE-2) were initiated and maintained on STO feeder cells. CT-1 and CT-5 were derived from the culture of intact, 10- to 11-day in vitro-produced blastocysts. CE-1 and CE-2 were derived from the culture of immunodissected inner cell masses of 7- to 8-day in vitro-produced blastocysts. The cultures were routinely passaged by physical dissociation. Although morphologically distinct, the trophectoderm and endoderm both grew as cell sheets of polarized epithelium (dome formations) composed of approximately cuboidal cells. Both cell types, particularly the endoderm, grew on top …

Similarity And Divergence Among Viruses In The Genus Furovirus, Yukio Shirako, Nobuhiro Suzuki, Roy C. French

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nucleotide sequences of RNAs 1 and 2 of a Japanese strain of soil-borne wheat mosaic virus (SBWMV), the type species of the genus Furovirus, and sorghum chlorotic spot virus (SCSV) were determined from cloned cDNA. The relationship among the Japanese and US strains of SBWMV, SCSV, oat golden stripe virus (OGSV), and recently proposed Chinese wheat mosaic and European wheat mosaic viruses (CWMV and EWMV) were examined at the nucleotide and amino acid levels. Pairwise comparisons of genome-encoded proteins among the six viruses showed that the US strains of SBWMV and CWMV were the most closely related pair in RNA …

Sistem Diagnosis Penyakit Tanaman Padi (Pakar Padi), Jin Gee Tee

Student Works (2000-2009)

Beras adalah makanan utama rakyat Malaysia. Banyak kajian dan penyelidikan dibuat untuk meningkatkan hasil tanaman padi. Di antaranya termasuklah mengkaji cara-cara kawalan serangga perosak dan kerja-kerja diagnosis penyakit tanaman padi. Maklumat-maklumat daripada hasil kajian harus disimpan dengan baik supaya kerja diagnosis dapat dijalankan dengan lebih lancar. Memandangkan keperluan ini, satu sistem berkomputeran yang menyimpan data data mengenai tanaman padi adalah penting dan dijangka akan memberi bantuan dalam pengurusan dan kerja-kerja diagnosis. Ini telah membawa kepada idea untuk membangunkan satu sistem diagnosis penyakit bagi tanaman padi. Sistem Diagnosis Penyakit Tanaman Padi, diberikan nama Pakar Padi adalah satu sistem berpangkalan komputer yang …

Biotechnology In Western Australian Agriculture, Agriculture Western Australia

Grain and other field crops published reports

The Government of Western Australia believes that the strategic use of biotechnology and particularly gene technology has the potential to offer Western Australian agriculture significant economic benefits by improving the productivity and quality of products, as well as contributing to the development of sustainable farming systems.

Information contained in these binders is intended to give an overview of Biotechnology in relation to what is occurring in Australia, in Western Australia and specifically, at Agriculture Western Australia (AGWEST).

Biotechnology has been around for twenty-five years or more, but has only recently become a public issue in Australia - particularly in relation …

Susceptibility Of The Legume Flower Thrips To Metarhizium Anisopliae On Different Varieties Of Cowpea, S. Ekesi, N. K. Maniania, W. Lwande

All Peer-Reviewed Publications

The susceptibility of Megalurothrips sjostedti to Metarhizium anisopliae when reared on susceptible, tolerant, and moderately resistant varieties of cowpea at different constant temperatures was evaluated in the laboratory. Insects were exposed either to direct spray of the conidia or to fungus-treated floral tissues. Mortality was significantly higher on the moderately resistant variety at all temperatures compared to the susceptible and tolerant varieties. Correspondingly, lethal time and lethal concentration values were significantly shorter and lower, respectively, on the moderately resistant variety compared to the other varieties, thus indicating that the two control methods are compatible as part of an integrated pest …

Evaluation Of Banana Cultivars For Resistance To The Banana Weevil Cosmopolites Sordidus And Nematode Complex In Western Kenya, T. Musabyimana, K. V. Seshu Reddy, L. Ngode, J. S. Prasad

All Peer-Reviewed Publications

Banana weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) and parasitic nematodes are often together on the same banana mat. Both pests have been recognized as major constraints to banana production. They may cause up to 85% yield loss in banana. Hence, both pests should be investigated together to define a control strategy for this complex constraint. With this view, our investigation was carried out in a naturally nematode-weevil infested farmer's field to search for host plant resistance to both pests. Pratylenchus goodeyi, Helicotylenchus multicinctus and Meloidogyne spp. were the most commonly occurring nematodes in the experiment and were often in mixed …

Powdered Neem Seed And Cake For Management Of The Banana Weevil, Cosmopolites Sordidus, And Parasitic Nematodes, T. Musabyimana, R. C. Saxena, E. W. Kairu, C. K.P.O. Ogol, Z. R. Khan

All Peer-Reviewed Publications

Field trials were conducted in Kenya with 'Nakyetengu', an East African highland banana cultivar (AAA-EA), highly susceptible to banana pests. Regardless of soil fertility levels, incorporation around the plant base of powdered neem (Azadirachta indica A. Juss.) seed or cake at 60-100 g/mat at 4-month intervals, gave better control of the banana weevil, Cosmopolites sordidus (Germar), and of parasitic nematodes, than that achieved with soil application of Furadan 5G (carbofuran) at 60 g/mat at 6-month intervals. Compared with untreated control, fruit yield in most of the neem treatments was significantly higher, particularly during the second cycle of crop production. Neem …

Effect Of Volatiles And Crude Extracts Of Different Plant Materials On Egg Viability Of Maruca Vitrata And Clavigralla Tomentosicollis, S. Ekesi

All Peer-Reviewed Publications

The effect of volatiles and aqueous extracts of black pepper, Piper guineense; neem seed, Azadirachta indica; garlic bulb, Allium sativum; and onion bulb, Allium cepa on egg viability of Maruca vitrata Fab. (Lepidoptera: Pyralidae) and Clavigralla tomentosicollis Stal (Heteroptera: Coreidae) was evaluated in laboratory experiments. Compared with the other treatments, volatiles of black pepper and garlic bulb were superior in reducing hatch of freshly laid (12-h-old) eggs of both species of insects. Volatiles of onion bulb and neem seed had no effect on egg hatch. When aqueous extracts of the various plant materials were tested at 5%, 10% and 15%, …