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Proceedings Of The 42nd Annual Meeting, Southern Soybean Disease Workers (March 11-12, 2015, Pensacola Beach, Florida), Craig Rothrock, Ed Sikora, Trey Price, Danise Beadle, Myra Purvis, Tom Allen, Loren Giesler

Southern Soybean Disease Workers: Conference Proceedings

Contents

Schedule

Southern United States Soybean Disease Loss Estimates for 2014. TW Allen, JP Damicone, NS Dufault, TR Faske, DE Hershman, CA Hollier, T Isakeit, RC Kemerait, NM Kleczewski, SR Koenning, HL Mehl, JD Mueller, C Overstreet, P Price, EJ Sikora, and H Young

Graduate student competition (Trey Price, moderator)

Effect of Secondary Nutrient Applications on Suppression of Charcoal Rot in Soybean. T Wilkerson, M Tomasu-Peterson, BR Golden, S Lu, AB Johnson, and TW Allen

Molecular Characterization of the G143A Mutation Leading to QoI Fungicide Resistance among Fungal Pathogens Causing Cercospora Leaf Blight and Purple Seed Stain of Soybean. S …

Search For Host Factors Involved In Attachment Of Agrobacterium Tumefaciens To Plants, Anna Petrovicheva

Dissertations, Theses, and Capstone Projects

Agrobacterium tumefaciens is able to infect a diverse array of plants and causes crown gall disease. Typically these bacteria attach to plant roots and transform the plant cells to induce tumors. The mechanism of this attachment in the infection process is not yet fully understood. Using wild type Arabidopsis thaliana, Columbia-0, and several Arabidopsis mutant lines as a binding target, we screened for A. thaliana mutants with altered adhesion.

The A. thaliana mutant lines were selected in The Arabidopsis Information Resource (TAIR) according to possible location of the resulting protein and similarity to known transformation mutants. Of these mutants …

Systematics And Biogeography Of Orthaea Kloztsch (Ericaceae: Vaccinieae), Nelson Salinas

Dissertations, Theses, and Capstone Projects

In the first chapter a study of the distribution patterns of the neotropical Vaccinieae (Ericaceae) is presented. Five areas of endemism were recovered: Central America, northern Chocó, southern Chocó, eastern Ecuador, and Yungas. Divergence times estimations indicate that the Andean clade of Vaccinieae migrated to South America during the Late Oligocene or Early Miocene (28.9-17.84 MA), and most of the subsequent diversification took place during the Tertiary. The Yungas was the first Andean area to be colonized, and several dispersals towards the north expanded their distribution range. Both areas of endemism and dispersal events were influenced by geological processes, such …

Bacterial Wilt Of Dry-Edible Beans In The Central High Plains Of The U.S.: Past, Present, And Future, Robert M. Harveson, Howard F. Schwartz, Carlos A. Urrea, C. Dean Yonts

Panhandle Research and Extension Center

Bacterial wilt, caused by Curtobacterium flaccumfaciens pv. flaccumfaciens, was first recognized and described as a new dry bean disease near Redfield, South Dakota, after the 1921 growing season on the farm of the Office of Forage Investigations. The grower on this farm informed the federal investigators that he believed the disease to be the same one that killed 90% of his crop in 1920. For the 1921 season, he saved and planted the seed harvested from the previous year, resulting in an estimated 25% loss.

. . .

It is likely that wilt has “reappeared” and become widely established throughout …

Testing Carbon Farming Opportunities For Salinity Management, Peter Ritson, Mike G. Clarke, Adele Killen, Sarah Jeffery

All other publications

An emerging prospect for farm revenue from revegetation of saline and other lands that are marginal or non-productive for agriculture is the sale of carbon credits. Australian Government schemes for carbon credits include the Carbon Farming Initiative (CFI) and the proposed Emissions Reduction Fund (ERF) Therefore, this study aimed to assess the potential for woody vegetation (trees and shrubs) established on and around salt-affected lands in the Northern Agricultural Region (NAR) to remove carbon dioxide from the atmosphere and store (sequester) the carbon in new growth. Revegetation plantings on six farms were selected for the study after the landowners expressed …

Potential Break Crops For Managing Root-Knot Nematode, Carla Wilkinson, Stuart Vincent, Sarah J. Collins Dr

Grain and Other Field Crops Research Articles

Western Australian growers may be unintentionally increasing root-knot nematode, Meloidogyne fallax (False Columbia root-knot nematode) populations by using susceptible break crops between cash crops.

Characterization Of Novel Sorghum Brown Midrib Mutants From An Ems-Mutagenized Population, Scott E. Sattler, Ana Saballos, Zhanguo Xin, Deanna L. Funnell-Harris, Wilfred Vermerris, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Reducing lignin concentration in lignocellulosic biomass can increase forage digestibility for

ruminant livestock and saccharification yields of biomass for bioenergy. In sorghum (Sorghum bicolor (L.) Moench) and several other C4 grasses, brown midrib (bmr) mutants have been shown to reduce lignin concentration. Putative bmr mutants isolated from an EMS-mutagenized population were characterized and classified based on their leaf midrib phenotype and allelism tests with the previously described sorghum bmr mutants bmr2, bmr6, and bmr12. These tests resulted in the identification of additional alleles of bmr2, bmr6, and bmr12, and, in addition, six bmr …

Assessment Of Rice Self-Sufficiency In 2025 In Eight African Countries, P. A.J. Van Oort, K. Saito, E. Amovin-Assagba, Lenny G.J. Van Bussel, Justin Van Wart, Hugo De Groot, Martin K. Van Ittersum, Kenneth Cassman, M. C.S. Wopereis

Department of Agronomy and Horticulture: Faculty Publications

Most African countries are far from self-sufficient in meeting their rice consumption; in eight countries the production: consumption ratio, ranged from 0.16 to 1.18 in 2012. We show that for the year 2025, with population growth, diet change and yield increase on existing land (intensification), countries cannot become fully self-sufficient in rice. This implies that for the future, a mixture of area expansion and imports will be needed on top of yield gap closure. Further research is needed for identification of most suitable new land for rice area expansion and areas that should be protected.

Patch Burning: Implications On Water Erosion And Soil Properties, Altingul Ozaslan Parlak, Mehmet Parlak, Humberto Blanco-Canqui, Walter H. Schacht, John A. Guretzky, Martha Mamo

Department of Agronomy and Horticulture: Faculty Publications

Patch burning can be a potential management tool to create grassland heterogeneity and enhance forage productivity and plant biodiversity, but its impacts on soil and environment have not been widely documented. In summer 2013, we studied the effect of time after patch burning (4 mo after burning [recently burned patches], 16 mo after burning [older burned patches], and unburned patches [control]) on vegetative cover, water erosion, and soil properties on a patch-burn experiment established in 2011 on a Yutan silty clay loam near Mead, NE. The recently burned patches had 29 ± 8.0% (mean ± SD) more bare ground, 21 …

School Gardens: Cultivating A Child’S Nutritional Habits, Environmental Knowledge, And Sustainability Practices, Jeffrey Meltzer

Honors Theses

School gardens have existed since the late nineteenth century and today are becoming increasingly popular in many parts of the world, including where I studied in Maine and Australia (AUS). Multiple organizations support school gardens in Maine, including the Maine School Garden Network, which has over 125 registered school gardens. In AUS, the Stephanie Alexander Kitchen Garden Foundation primarily supports the school garden movement and has over 800 registered school gardens. While many researchers have studied school gardens, few have compared two countries, focused on environmental sustainability, or investigated Maine in particular. This thesis combines information from literature reviews, and …

Transgenic Wheat Expressing A Barley Udp-Glucosyltransferase Detoxifies Deoxynivalenol And Provides High Levels Of Resistance To Fusarium Graminearum, Xi Li, Sanghyun Shin, Shane Heinen, Ruth Dill-Macky, Franz Berthiller, Natalya Nersesian, Thomas Clemente, Susan Mccormick, Gary J. Muehbauer

Department of Agronomy and Horticulture: Faculty Publications

Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a devastating disease of wheat that results in economic losses worldwide. During infection, F. graminearum produces trichothecene mycotoxins, including deoxynivalenol (DON), that increase fungal virulence and reduce grain quality. Transgenic wheat expressing a barley UDP-glucosyltransferase (HvUGT13248) were developed and evaluated for FHB resistance, DON accumulation, and the ability to metabolize DON to the less toxic DON-3-O-glucoside (D3G). Pointinoculation tests in the greenhouse showed that transgenic wheat carrying HvUGT13248 exhibited significantly higher resistance to disease spread in the spike (type II resistance) compared with nontransformed controls. Two transgenic …

Fall Seed Guide 2015, Teshome Regassa, P. Stephen Baenziger, Stephen N. Wegulo, Greg Kruger, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Crops included in this guide are winter wheat, winter barley, and triticale. You may receive this guide in the mail or through the University of Nebraska Extension network. The data and other information this guide is can be found at our web site: http://cropwatch.unl.edu/varietytest/ and http://www.unl.edu/ncia. Additional information is available at the wheat variety virtual tour web site http://cropwatch.unl.edu/wheat/virtual or the winter wheat variety selection tool page http:// citnews.unl.edu/winter_wheat_tool/index.shtml. The last season was hard on Nebraska winter wheat. Emergence was poor in the west due to dry conditions. On the other hand, some plots in the east had to be …

Model And Sensor-Based Recommendation Approaches For In-Season Nitrogen Management In Corn, L. J. Thompson, R. B. Ferguson, N. Kitchen, D. W. Frazen, M. Mamo, H. Yang, J. S. Schepers

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen management for corn (Zea mays L.) may be improved by applying a portion of N in-season. This investigation was conducted to evaluate crop modeling (Maize-N) and active crop canopy sensing approaches for recommending in-season N fertilizer rates. These approaches were evaluated during 2012–2013 on 11 field sites, in Missouri, Nebraska, and North Dakota. Nitrogen management also included a no-N treatment (check) and a non-limiting N reference (all at planting). Nitrogen management treatments were assessed for two hybrids and at low and high seeding rates, arranged in a randomized complete block design. In 9 of 11 site-years, the sensor-based …

Potential For Crop Production Increase In Argentina Through Closure Of Existing Yield Gaps, Fernando Aramburu Merlos, Juan Pablo Monzon, Jorge L. Mercau, Miguel Taboada, Fernando H. Andrade, Antonio J. Hall, Esteban Jobbagy, Kenneth Cassman, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Favorable climate and soils for rainfed crop production, together with a relatively low population density,results in 70–90% of Argentina grain production being exported. No assessment to date has tried to estimate the potential for extra grain production for soybean, wheat and maize, which account for 78%of total harvested area, by yield gap closure on existing cropland area and its impact at a global scale.The objectives of this paper are (i) to estimate how much additional grain could be produced without expanding crop area by closing yield gaps in Argentina, (ii) to investigate how this production and yield gaps varies across …

Plants Coping Abiotic And Biotic Stresses: A Tale Of Diligent Management, Hatem Rouached, Sikander Pal, Shimon Rachmilevitch, Marc Libault, Lam-Son Phan Tran

Department of Agronomy and Horticulture: Faculty Publications

Plants unlike other living forms are sessile thereby facing severe biotic and abiotic stresses. Plants have evolved different efficient defence responses which thrive upon a number of intrinsic factors, such as genotypic and phenotypic constitutions and developmental circumstances, and extrinsic factors like severity and duration of the stresses. Stress management uses molecular and biochemical level controls, the competence, and speed, at which a stress signal is perceived and transmitted to generate stress signal molecules and activate stress-protective mechanisms. A well-concerted action of the plants’ competence at morphological, physiological, biochemical, and molecular strata regulates numerous adaptive responses to biotic and abiotic …

Calibration And Validation Of The Hybrid-Maize Crop Model For Regional Analysis And Application Over The U.S. Corn Belt, Xing Liu, Jeff Andresen, Haishun Yang, Dev Niyogi

Department of Agronomy and Horticulture: Faculty Publications

Detailed parameter sensitivity, model validation, and regional calibration of the Hybrid-Maize crop model were undertaken for the purpose of regional agroclimatic assessments. The model was run at both field scale and county scale. The county-scale study was based on 30-yr daily weather data and corn yield data from the National Agricultural Statistics Service survey for 24 locations across the Corn Belt of the United States. The field-scale study was based on AmeriFlux sites at Bondville, Illinois, andMead, Nebraska. By using the one-at-a-time and interaction-explicit factorial design approaches for sensitivity analysis, the study found that the five most sensitive parameters of …

Nutrient Availability And Dynamics Of Compost Bedded Pack Dairy Barn Waste, Leslie Hammond

Theses and Dissertations--Plant and Soil Sciences

Increased adoption of compost bedded pack dairy barns (CBP), a relatively new housing and manure handling strategy, warrants study to facilitate proper use of CBP waste as a soil amendment. This study: 1) characterized in situ nutrient content and availability of CBP waste in terms of nitrogen (N) and phosphorus (P); 2) examined the decomposition of surface-applied CBP waste compared to an alternative processed solid waste amendment; 3) examined nutrient dynamics of incorporated CBP waste in high and low soil test phosphorus (STP) environments. Quick anaerobic mineralization assays revealed that bed management affects nutrient content and availability of CBP waste. …

Identification Of Novel Qtl Governing Root Architectural Traits In An Interspecific Soybean Population, Lakshmi P. Manavalan, Silvas J. Prince, Theresa A. Musket, Julian Chaky, Rupesh Deshmukh, Tri D. Vuong, Li Song, Perry B. Cregan, James C. Nelson, J. Grover Shannon, James E. Specht, Henry T. Nguyen

Department of Agronomy and Horticulture: Faculty Publications

Cultivated soybean (Glycine max L.) cv. Dunbar (PI 552538) and wild G. soja (PI 326582A) exhibited significant differences in root architecture and root-related traits. In this study, phenotypic variability for root traits among 251 BC₂F₅ backcross inbred lines (BILs) developed from the cross Dunbar/PI 326582A were identified. The root systems of the parents and BILs were evaluated in controlled environmental conditions using a cone system at seedling stage. The G. max parent Dunbar contributed phenotypically favorable alleles at a major quantitative trait locus on chromosome 8 (Satt315-I locus) that governed root traits (tap root length and …

Genetic And Molecular Characterization Of Submergence Response Identifies Subtol6 As A Major Submergence Tolerance Locus In Maize, Malachy T. Campbell, Christopher A. Proctor, Yongchao Dou, Aaron J. Schmitz, Piyaporn Phansak, Greg R. Kruger, Chi Zhang, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Maize is highly sensitive to short term flooding and submergence. Early season flooding reduces germination, survival and growth rate of maize seedlings. We aimed to discover genetic variation for submergence tolerance in maize and elucidate the genetic basis of submergence tolerance through transcriptional profiling and linkage analysis of contrasting genotypes. A diverse set of maize nested association mapping (NAM) founder lines were screened, and two highly tolerant (Mo18Wand M162W) and sensitive (B97 and B73) genotypes were identified. Tolerant lines exhibited delayed senescence and lower oxidative stress levels compared to sensitive lines. Transcriptome analysis was performed on these inbreds to provide …

Allmaps: Robust Scaffold Ordering Based On Multiple Maps, Haibo Tang, Xingtan Zhang, Chenyong Miao, Jisen Zhang, Ray Ming, James C. Schnable, Patrick S. Schnable, Eric Lyons, Jianguo Lu

Department of Agronomy and Horticulture: Faculty Publications

The ordering and orientation of genomic scaffolds to reconstruct chromosomes is an essential step during de novo genome assembly. Because this process utilizes various mapping techniques that each provides an independent line of evidence, a combination of multiple maps can improve the accuracy of the resulting chromosomal assemblies. We present ALLMAPS, a method capable of computing a scaffold ordering that maximizes colinearity across a collection of maps. ALLMAPS is robust against common mapping errors, and generates sequences that are maximally concordant with the input maps. ALLMAPS is a useful tool in building high-quality genome assemblies. ALLMAPS is available at: https://github.com/tanghaibao/jcvi/wiki/ALLMAPS.

From Field To Atlas: Upscaling Of Location-Specific Yield Gap Estimates, Lenny G.J. Van Bussel, Patricio Grassini, Justin Van Wart, Joost Wolf, Lieven Claessens, Haishun Yang, Hendrik Boogaard, Hugo De Groot, Kazuki Saito, Kenneth Cassman, Martin K. Van Ittersum

Department of Agronomy and Horticulture: Faculty Publications

Accurate estimation of yield gaps is only possible for locations where high quality local data are available,which are, however, lacking in many regions of the world. The challenge is how yield gap estimates based on location-specific input data can be used to obtain yield gap estimates for larger spatial areas. Hence,insight about the minimum number of locations required to achieve robust estimates of yield gaps at larger spatial scales is essential because data collection at a large number of locations is expensive and time consuming. In this paper we describe an approach that consists of a climate zonation scheme supplemented …

Effects Of Sowing Date On Phenotypic Plasticity Of Fitness-Related Traits In Two Annual Weeds On The Songnen Plain Of China, Haiyan Li, John L. Lindquist, Yunfei Yang

Department of Agronomy and Horticulture: Faculty Publications

Background Phenotypic plasticity of fitness-related traits is vital for plant species to adapt to variable environments. Chenopodium glaucum L. and Amaranthus retroflexus L. are two common weed species globally. Understanding the plasticity in life-history traits, especially in reproductive allocation, within and among these species is important for predicting their success and for managing them in different environments.

Methodology/Principal Findings Seeds of the two plant species were sown every 10 days from 26 Jun to 15 Aug. Life-history and fitness-related traits of both phenology and morphology were measured, and dry biomass of roots, stems, leaves, and reproductive tissues was determined at …

Fingerprinting Soybean Germplasm And Its Utility In Genomic Research, Qijian Song, D. L. Hyten, Gaofeng Jia, Charles V. Quigley, Edward W. Fickus, Randall L. Nelson, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

The United States Department of Agriculture, Soybean Germplasm Collection includes 18,480 domesticated soybean and 1,168 wild soybean accessions introduced from 84 countries or developed in the U.S. This collection was genotyped with the SoySNP50K BeadChip containing greater than 50K SNPs. Redundant accessions were identified in the collection and distinct genetic backgrounds of soybean from different geographic origins were observed that could be a unique resource for soybean genetic improvement. We detected a dramatic reduction of genetic diversity based on linkage disequilibrium and haplotype structure analyses of the wild, landrace and North American cultivar populations and identified candidate regions associated with …

Selective Genotyping For Marker Assisted Selection Strategies For Soybean Yield Improvement, Benjamin D. Fallen, Fred L. Allen, Dean A. Kopsell, Arnold M. Saxton, Leah Mchale, J. Grover Shannon, Stella K. Kantartzi, Andrea J. Cardinal, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone

Department of Agronomy and Horticulture: Faculty Publications

Using molecular markers in soybean [Glycine max (L.) Merr.] has lead to the identification of major loci controlling quantitative and qualitative traits that include: disease resistance, insect resistance and tolerance to abiotic stresses. Yield has been considered as one of the most important quantitative traits in soybean breeding. Unfortunately, yield is a very complex trait and most yield quantitative trait loci (QTL) that have been identified have had only limited success for marker assisted selection (MAS). The objective of this study was to identify QTL associated with soybean seed yield in preliminary yield trials grown in different environments and …

Qtl For Seed Protein And Amino Acids In The Benning × Danbaekkong Soybean Population, C. V. Warrington, H. Abdel‑Haleem, D. L. Hyten, P. B. Cregan, J. H. Orf, A. S. Killam, N. Bajjalieh, Z. Li, H. R. Boerma

Department of Agronomy and Horticulture: Faculty Publications

Soybean, rather than nitrogen-containing forages, is the primary source of quality protein in feed formulations for domestic swine, poultry, and dairy industries. As a sole dietary source of protein, soybean is deficient in the amino acids lysine (Lys), threonine (Thr), methionine (Met), and cysteine (Cys). Increasing these amino acids would benefit the feed industry. The objective of the present study was to identify quantitative trait loci (QTL) associated with crude protein (cp) and amino acids in the ‘Benning’ × ‘Danbaekkong’ population. The population was grown in five southern USA environments. Amino acid concentrations as a fraction of cp (Lys/cp, Thr/cp, …

Using A Simple Leaf Color Chart To Estimate Leaf And Canopy Chlorophyll A Content In Maize (Zea Mays), Anthony Nguy-Robertson, Yi Peng, Timothy Arkebauer, David Scoby, James Schepers, Anatoly Gitelson

Department of Agronomy and Horticulture: Faculty Publications

This study utilized a leaf color chart (LCC) to characterize the variation in leaf chlorophyll and estimate canopy chlorophyll in maize (Zea mays). The LCC consisted of four levels of greenness and was used to sort maize leaves in 2011 for three fields near Mead, Nebraska, USA. Leaf chlorophyll content for each color chart class was determined using two leaf-level sensors. The variation within each LCC class was reasonable (CV < 56%). The darkest color class predominated and indicated adequate fertilization rates using a SPAD. Canopy chlorophyll content was estimated using destructively measured leaf area index (LAI) and the LCC. This approach was verified with a method utilizing canopy reflectance collected by both satellite imagery and a four-band radiometer. The error between the two methods was reasonable (RMSE = 0.55-0.88 g m^-2; CV = 25.6-50.4%), indicating that both leaf and canopy chlorophyll can be estimated cheaply without a wet lab or field-based sensors.

First Approximations Of Prescribed Fire Risks Relative To Other Management Techniques Used On Private Lands, Dirac L. Twidwell, Carissa L. Wonkka, Michael T. Sindelar, John R. Weir

Department of Agronomy and Horticulture: Faculty Publications

Fire is widely recognized as a critical ecological and evolutionary driver that needs to be at the forefront of land management actions if conservation targets are to be met. However, the prevailing view is that prescribed fire is riskier than other land management techniques. Perceived risks associated with the application of fire limits its use and reduces agency support for prescribed burning in the private sector. As a result, considerably less cost-share support is given for prescribed fire compared to mechanical techniques. This study tests the general perception that fire is a riskier technique relative to other land management options. …

A Genomic Selection Index Applied To Simulated And Real Data, J. Jesus Ceron-Rojas, Jose Crossa, Vivi N. Arief, Kaye Basford, Jessica Rutkoski, Diego Jarquin, Gregorio Alvarado, Yoseph Beyene, Kassa Semagn, Ian Delacy

Department of Agronomy and Horticulture: Faculty Publications

A genomic selection index (GSI) is a linear combination of genomic estimated breeding values that uses genomic markers to predict the net genetic merit and select parents from a nonphenotyped testing population. Some authors have proposed a GSI; however, they have not used simulated or real data to validate the GSI theory and have not explained how to estimate the GSI selection response and the GSI expected genetic gain per selection cycle for the unobserved traits after the first selection cycle to obtain information about the genetic gains in each subsequent selection cycle. In this paper, we develop the theory …

Understanding Rice Adaptation To Varying Agro-Ecosystems: Trait Interactions And Quantitative Trait Loci, Shalabh Dixit, Alexandre Grondin, Cheng-Ruei Lee, Amelia Henry, Thomas-Mitchell Olds, Arvind Kumar

Department of Agronomy and Horticulture: Faculty Publications

Background: Interaction and genetic control for traits influencing the adaptation of the rice crop to varying environments was studied in a mapping population derived from parents (Moroberekan and Swarna) contrasting for drought tolerance, yield potential, lodging resistance, and adaptation to dry direct seeding. A BC₂F₃-derived mapping population for traits related to these four trait groups was phenotyped to understand the interactions among traits and to map and align QTLs using composite interval mapping (CIM). The study also aimed to identify QTLs for the four trait groups as composite traits using multivariate least square interval mapping (MLSIM) …

Overexpression Of Sbmyb60 Impacts Phenylpropanoid Biosynthesis And Alters Secondary Cell Wall Composition In Sorghum Bicolor, Erin D. Scully, Tammy Gries, Gautam Sarath, Nathan A. Palmer, Lisa Baird, Michelle J. Serapiglia, Bruce S. Dien, Akwasi A. Boateng, Zhengxiang Ge, Deanna L. Funnell-Harris, Paul Twigg, Thomas E. Clemente, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

The phenylpropanoid biosynthetic pathway that generates lignin subunits represents a significant target for altering the abundance and composition of lignin. The global regulators of phenylpropanoid metabolism may include MYB transcription factors, whose expression levels have been correlated with changes in secondary cell wall composition and the levels of several other aromatic compounds, including anthocyanins and flavonoids. While transcription factors correlated with downregulation of the phenylpropanoid biosynthesis pathway have been identified in several grass species, few transcription factors linked to activation of this pathway have been identified in C4 grasses, some of which are being developed as dedicated bioenergy feedstocks. In …