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- Bacterial artificial chromosome (BAC) clone end sequences (BES)Simple sequence repeats (SSRs)Plant disease resistance (R) genesNucleotide binding site targeted sequencingResistance gene analogs (1)
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Articles 1 - 13 of 13
Full-Text Articles in Plant Sciences
Using Plant Volatile Traps To Develop Phenology Models For Natural Enemies: An Example Using Chrysopa Nigricornis (Burmeister) (Neuroptera: Chrysopidae), Vincent P. Jones, David R. Horton, Nicholas J. Mills, Thomas R. Unruh, Eugene R. Milickzy, Peter W. Shearer, Kaushalya G. Amarasekare, Callie C. Baker, Tawnee D. Melton
Using Plant Volatile Traps To Develop Phenology Models For Natural Enemies: An Example Using Chrysopa Nigricornis (Burmeister) (Neuroptera: Chrysopidae), Vincent P. Jones, David R. Horton, Nicholas J. Mills, Thomas R. Unruh, Eugene R. Milickzy, Peter W. Shearer, Kaushalya G. Amarasekare, Callie C. Baker, Tawnee D. Melton
Agricultural and Environmental Sciences Faculty Research
A model predicting phenology of adult Chrysopa nigricornis (Burmeister) (Neuroptera: Chrysopidae) in orchards was developed from field (trapping) data supplemented with development data collected under laboratory conditions. Lower and upper thresholds of 10.1°C and 29.9°C, respectively, were estimated from published and unpublished laboratory observations, and were used to develop a phenology model. Season-long field data were collected using white delta traps that had been baited with squalene, a volatile shown previously to be highly attractive to C. nigricornis. The model was developed from data collected in three Washington apple orchards, and was validated using independent data sets collected from apple, …
Interplanting Annual Ryegrass, Wheat, Oat, And Corn To Mitigate Iron Deficiency In Dry Beans, Emmanuel Chiwo Omondi, Andrew R. Kniss
Interplanting Annual Ryegrass, Wheat, Oat, And Corn To Mitigate Iron Deficiency In Dry Beans, Emmanuel Chiwo Omondi, Andrew R. Kniss
Agricultural and Environmental Sciences Faculty Research
This study evaluated whether grass intercropping can be used to alleviate Fe deficiency chlorosis in dry beans (Phaseolus vulgaris L.) grown in high pH, calcareous soils with low organic matter. Field studies were conducted at the University of Wyoming Sustainable Agriculture Research and Extension Center in 2009 and 2010. Black- and navy beans were grown alone or intercropped with annual ryegrass (Lolium multiflorum Lam.), oat (Avena sativa L.), corn (Zea mays L.), or spring wheat (Triticum aestivum L.) in a two-factor factorial strip-plot randomized complete block design. All four grass species increased chlorophyll intensity in dry beans. However, grass species …
Soil Carbon And Nitrogen Mineralization And Crop Parameters In Typical Maize-Bean Intercropping In Western Kenya, Judith A. Odhiambo, Urszula Norton, Emmanuel C. Omondi, Dennis Ashilenje, Jay B. Norton
Soil Carbon And Nitrogen Mineralization And Crop Parameters In Typical Maize-Bean Intercropping In Western Kenya, Judith A. Odhiambo, Urszula Norton, Emmanuel C. Omondi, Dennis Ashilenje, Jay B. Norton
Agricultural and Environmental Sciences Faculty Research
Smallholder farmers in western Kenya who plant maize (Zea mays L.) intercropped with beans (Phaseolus vulgaris L.) face many challenges associated with nutrient-poor soils and weather-related crop failures. In regions where temperatures are favorable, crops are grown twice per year during long and short rainy seasons and in other regions, once per year during one long growing season. Growing crops two times per year necessitates frequent land preparation using inversion-type tillage. Little is known about the impact of current tillage-intensive crop management on annual soil organic matter (SOM) recovery. The aim of this study was to assess changes to soil …
Nitrous Oxide Emissions From A Commercial Cornfield (Zea Mays) Measured Using The Eddy Covariance Technique, H. Huang, J. Wang, D. Hui, D. R. Miller, S. Bhattarai, S. Dennis, D. Smart, T. Sammis, K. C. Reddy
Nitrous Oxide Emissions From A Commercial Cornfield (Zea Mays) Measured Using The Eddy Covariance Technique, H. Huang, J. Wang, D. Hui, D. R. Miller, S. Bhattarai, S. Dennis, D. Smart, T. Sammis, K. C. Reddy
Agricultural and Environmental Sciences Faculty Research
Increases in observed atmospheric concentrations of the long-lived greenhouse gas nitrous oxide (N2O) have been well documented. However, information on event-related instantaneous emissions during fertilizer applications is lacking. With the development of fast-response N2O analyzers, the eddy covariance (EC) technique can be used to gather instantaneous measurements of N2O concentrations to quantify the exchange of nitrogen between the soil and atmosphere. The objectives of this study were to evaluate the performance of a new EC system, to measure the N2O flux with the system, and finally to examine relationships of the N2O flux with soil temperature, soil moisture, precipitation, and …
Legume Crops Phylogeny And Genetic Diversity For Science And Breeding, Petr Smýkal, Clarice J. Coyne, Mike J. Ambrose, Nigel Maxted, Hanno Schaefer, Matthew W. Blair
Legume Crops Phylogeny And Genetic Diversity For Science And Breeding, Petr Smýkal, Clarice J. Coyne, Mike J. Ambrose, Nigel Maxted, Hanno Schaefer, Matthew W. Blair
Agricultural and Environmental Sciences Faculty Research
Economically, legumes (Fabaceae) represent the second most important family of crop plants after the grass family, Poaceae. Grain legumes account for 27% of world crop production and provide 33% of the dietary protein consumed by humans, while pasture and forage legumes provide vital part of animal feed. Fabaceae, the third largest family of flowering plants, has traditionally been divided into the following three subfamilies: Caesalpinioideae, Mimosoideae, and Papilionoideae, all together with 800 genera and 20,000 species. The latter subfamily contains most of the major cultivated food and feed crops. Among the grain legumes are some of mankind's earliest crop plants, …
Bac-End Microsatellites From Intra And Inter-Genic Regions Of The Common Bean Genome And Their Correlation With Cytogenetic Features, Matthew Wohlgemuth Blair, Juana Marcela Córdoba, Claritza Muñóz, Deissy K. Yuyó
Bac-End Microsatellites From Intra And Inter-Genic Regions Of The Common Bean Genome And Their Correlation With Cytogenetic Features, Matthew Wohlgemuth Blair, Juana Marcela Córdoba, Claritza Muñóz, Deissy K. Yuyó
Agricultural and Environmental Sciences Faculty Research
Highly polymorphic markers such as simple sequence repeats (SSRs) or microsatellites are very useful for genetic mapping. In this study novel SSRs were identified in BAC-end sequences (BES) from non-contigged, non-overlapping bacterial artificial clones (BACs) in common bean (Phaseolus vulgaris L.). These so called “singleton” BACs were from the G19833 Andean gene pool physical map and the new BES-SSR markers were used for the saturation of the inter-gene pool, DOR364×G19833 genetic map. A total of 899 SSR loci were found among the singleton BES, but only 346 loci corresponded to the single di- or tri-nucleotide motifs that were likely …
Organic Treatments For Control Of Pepper Weevil (Coleoptera: Curculionidae), Karla M. Addesso, Philip A. Stansly, Barry C. Kostyk, Heather J. Mcauslane
Organic Treatments For Control Of Pepper Weevil (Coleoptera: Curculionidae), Karla M. Addesso, Philip A. Stansly, Barry C. Kostyk, Heather J. Mcauslane
Agricultural and Environmental Sciences Faculty Research
The pepper weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae) is a major pest of pepper (Capsicum spp.; Solanales: Solanaceae) in the southern United States, Mexico, Central America and the Caribbean. Feeding and oviposition cause flower and fruit abscission and internal fruit damage resulting in serious yield losses. Females lay eggs in flower buds and small fruits, shielding larvae from contact pesticides, leaving only the adult stage vulnerable. The purpose of this study was to investigate low-risk and organic products for use against the pepper weevil to provide both organic and conventional growers with more control options. A neem product (Ecozin® …
Life History Of Paracoccus Marginatus (Hemiptera: Pseudococcidae) On Four Host Plant Species Under Laboratory Conditions, Kaushalya G. Amarasekare, Catharine M. Mannion, Lance S. Osborne, Nancy D. Epsky
Life History Of Paracoccus Marginatus (Hemiptera: Pseudococcidae) On Four Host Plant Species Under Laboratory Conditions, Kaushalya G. Amarasekare, Catharine M. Mannion, Lance S. Osborne, Nancy D. Epsky
Agricultural and Environmental Sciences Faculty Research
Life history of the mealybug, Paracoccus marginatus Williams and Granara de Willink, on three ornamental plants [Hibiscus rosa-sinensis L., Acalypha wilkesiana (Muell.-Arg.), and Plumeria rubra L.] and one weed species (Parthenium hysterophorus L.) was studied under laboratory conditions. Mealybugs were able to develop, survive, and reproduce on all four hosts; however, there were differences in the life history parameters. Adult females that developed on acalypha and parthenium emerged ≈1 d earlier than those that developed on hibiscus and plumeria. Adult males had a longer developmental time on plumeria than on the other hosts. Survival of first- and second-instar …
Development And Mapping Of Ssr Markers Linked To Resistance-Gene Homologue Clusters In Common Bean, Luz Nayibegarzon, Matthew Wohlgemuth Blair
Development And Mapping Of Ssr Markers Linked To Resistance-Gene Homologue Clusters In Common Bean, Luz Nayibegarzon, Matthew Wohlgemuth Blair
Agricultural and Environmental Sciences Faculty Research
Common bean is an important but often a disease-susceptible legume crop of temperate, subtropical and tropical regions worldwide. The crop is affected by bacterial, fungal and viral pathogens. The strategy of resistance-gene homologue (RGH) cloning has proven to be an efficient tool for identifying markers and R (resistance) genes associated with resistances to diseases. Microsatellite or SSR markers can be identified by physical association with RGH clones on large-insert DNA clones such as bacterial artificial chromosomes (BACs). Our objectives in this work were to identify RGH-SSR in a BAC library from the Andean genotype G19833 and to test and map …
The Role Of The Testa During Development And In Establishment Of Dormancy Of The Legume Seed, Petr Smýkal, Vanessa Vernoud, Matthew W. Blair, Aleš Soukup, Richard D. Thompson
The Role Of The Testa During Development And In Establishment Of Dormancy Of The Legume Seed, Petr Smýkal, Vanessa Vernoud, Matthew W. Blair, Aleš Soukup, Richard D. Thompson
Agricultural and Environmental Sciences Faculty Research
Timing of seed germination is one of the key steps in plant life cycles. It determines the beginning of plant growth in natural or agricultural ecosystems. In the wild, many seeds exhibit dormancy and will only germinate after exposure to certain environmental conditions. In contrast, crop seeds germinate as soon as they are imbibed usually at planting time. These domestication-triggered changes represent adaptations to cultivation and human harvesting. Germination is one of the common sets of traits recorded in different crops and termed the “domestication syndrome.” Moreover, legume seed imbibition has a crucial role in cooking properties. Different seed dormancy …
Comparison Of Five Major Trichome Regulatory Genes In Brassica Villosa With Orthologues Within The Brassicaceae, Naghabushana K. Nayidu, Sateesh Kagale, Ali Taheri, Thushan S. Withana-Gamage, Isobel A. P. Parkin, Andrew G. Sharpe, Margaret Y. Gruber
Comparison Of Five Major Trichome Regulatory Genes In Brassica Villosa With Orthologues Within The Brassicaceae, Naghabushana K. Nayidu, Sateesh Kagale, Ali Taheri, Thushan S. Withana-Gamage, Isobel A. P. Parkin, Andrew G. Sharpe, Margaret Y. Gruber
Agricultural and Environmental Sciences Faculty Research
Coding sequences for major trichome regulatory genes, including the positive regulators GLABRA 1(GL1), GLABRA 2 (GL2), ENHANCER OF GLABRA 3 (EGL3), and TRANSPARENT TESTA GLABRA 1 (TTG1) and the negative regulator TRIPTYCHON (TRY), were cloned from wild Brassica villosa, which is characterized by dense trichome coverage over most of the plant. Transcript (FPKM) levels from RNA sequencing indicated much higher expression of the GL2 and TTG1 regulatory genes in B. villosa leaves compared with expression levels of GL1 and EGL3 genes in either B. villosa or the reference genome species, glabrous B. oleracea; however, cotyledon TTG1 expression was high in …
Effect Of Aluminum Treatment On Proteomes Of Radicles Of Seeds Derived From Al-Treated Tomato Plants, Ikenna Okekeogbu, Zhujia Ye, Sasikiran Sangireddy, Hui Li, Sarabjit Bhatti, Dafeng Hui, Kevin J. Howe, Tara Fish, Yong Yang, Theodore W. Thannhauser
Effect Of Aluminum Treatment On Proteomes Of Radicles Of Seeds Derived From Al-Treated Tomato Plants, Ikenna Okekeogbu, Zhujia Ye, Sasikiran Sangireddy, Hui Li, Sarabjit Bhatti, Dafeng Hui, Kevin J. Howe, Tara Fish, Yong Yang, Theodore W. Thannhauser
Agricultural and Environmental Sciences Faculty Research
Aluminum (Al) toxicity is a major constraint to plant growth and crop yield in acid soils. Tomato cultivars are especially susceptible to excessive Al3+ accumulated in the root zone. In this study, tomato plants were grown in a hydroponic culture system supplemented with 50 µM AlK(SO4)2. Seeds harvested from Al-treated plants contained a significantly higher Al content than those grown in the control hydroponic solution. In this study, these Al-enriched tomato seeds (harvested from Al-treated tomato plants) were germinated in 50 µM AlK(SO4)2 solution in a homopiperazine-1,4-bis(2-ethanesulfonic acid) buffer (pH 4.0), and …
Stem Cutting Propagation In Whole Pine Tree Substrates, Anthony L. Witcher, Eugene K. Blythe, Glenn B. Fain, Kenneth J. Curry
Stem Cutting Propagation In Whole Pine Tree Substrates, Anthony L. Witcher, Eugene K. Blythe, Glenn B. Fain, Kenneth J. Curry
Agricultural and Environmental Sciences Faculty Research
Wood-based substrates have been extensively evaluated for greenhouse and nursery crop production, yet these substrates have not been evaluated for propagation. The objective of this study was to evaluate processed whole loblolly pine trees (WPT) (Pinus taeda) as a rooting substrate for stem cutting propagation of a range of ornamental crops. Substrates included processed WPT, pine (Pinus sp.) bark (PB), and each mixed with equal parts (by volume) peatmoss (PM) (WPT:PM and PB:PM, respectively). Substrate physical (air space, container capacity, total porosity, bulk density, and particle size distribution) and chemical [pH and electrical conductivity (EC)] properties were determined for all …