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Bacterial artificial chromosome (BAC) clone end sequences (BES)Simple sequence repeats (SSRs)Plant disease resistance (R) genesNucleotide binding site targeted sequencingResistance gene analogs
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Development And Mapping Of Ssr Markers Linked To Resistance-Gene Homologue Clusters In Common Bean, Luz Nayibegarzon, Matthew Wohlgemuth Blair
Development And Mapping Of Ssr Markers Linked To Resistance-Gene Homologue Clusters In Common Bean, Luz Nayibegarzon, Matthew Wohlgemuth Blair
Agricultural and Environmental Sciences Faculty Research
Common bean is an important but often a disease-susceptible legume crop of temperate, subtropical and tropical regions worldwide. The crop is affected by bacterial, fungal and viral pathogens. The strategy of resistance-gene homologue (RGH) cloning has proven to be an efficient tool for identifying markers and R (resistance) genes associated with resistances to diseases. Microsatellite or SSR markers can be identified by physical association with RGH clones on large-insert DNA clones such as bacterial artificial chromosomes (BACs). Our objectives in this work were to identify RGH-SSR in a BAC library from the Andean genotype G19833 and to test and map …