Physiology Commons^™

Phytochemical Analysis, Antioxidant, And Antibacterial Properties Of Partition Fractions Of Adansonia Digitata And Annona Muricata Extracts Using Chloroform, Ethyl Acetate, Ethanol, And Aqueous Solvent Systems, Fagbohun Oyenike Bushirat, Hassan Abdusalam Adewuyi, Sakariyau Adio Waheed, Maryam Nana Musa, Timothy God-Giveth Olusegun, Ayomide Babatunde Ishola, Agumage Idoko, Adeola Victor Kolawole, Adesanmi Adefunmilayo Oluwatuyi, Sarah Ngozi Agwasim

Chemistry & Biochemistry Faculty Publications

Adansonia digitata and Annona muricata are traditionally used medicinal plants with reported pharmacological properties. In this study, we aimed to elucidate the phytochemical composition, antioxidant activity, and antibacterial properties of partition fractions of Adansonia digitata and Annona muricata extracts obtained using chloroform, ethyl acetate, ethanol, and aqueous solvent systems. The plant extracts were successively partitioned using chloroform, ethyl acetate, ethanol, and aqueous solvent systems. Phytochemical analysis was performed using standard methods. Antioxidant activity was evaluated using FRAP and DPPH assays. Antibacterial activity was assessed using agar well diffusion and MIC determination. Phytochemical analysis revealed the presence of alkaloids (10.2-15.6%), flavonoids …

The Role Of Aquaporin-3 Lysine 282 Acetylation In Renal Water Balance, Nha Van Huynh

All ETDs from UAB

Aquaporin-3 (AQP3) mediates basolateral water transport in the kidney collecting duct principal cells, contributing to vasopressin-mediated urine concentration. We previously identified a novel post-translational modification of AQP3, the acetylation of lysine 282 (K282), which we hypothesized as a positive regulator of AQP3 water permeability. First, we found that collecting duct K282 acetylated AQP3 increased upon water deprivation, suggesting an involvement in renal water balance. To decipher the functional role of this modification, we utilized AQP3 acetylation (K282Q or Q) and deacetylation (K282R or R) mimetic mutant mice models generated using CRISPR/Cas9 knock-in technology. Both male and female wild-type (WT) and …

Cellphone Separation Modulates The Effects Of Working Memory Load On Ex-Gaussian Parameters Of Choice Reaction Time, Michael Gazzanigo, Alexa Quesnel, Catalina Roldan, Xiao Yang

Psychology Faculty Publications

Cognitive effects of cellphone dependency among young adults have garnered increasing research attention. While cellphones have been identified as a distractor in daily tasks, related psychological processes remain unclear. As a potential mechanism underlying those effects of cellphones, excessive working memory (WM) load has not yet been well examined. Our study investigated the effects of the mental representation of cellphone separation on WM. Seventy-five participants (M(age) = 21.3 years; 55 females, 20 males) were assigned into three groups: the cued separation, natural separation, or control group, and completed a block of choice reaction time (CRT) task, and a dual-task block: …

Use Of Coelomic Fluid As A Noninvasive Indicator Of Reproductive Status In Elasmobranchs, Emily Sapp

UNF Graduate Theses and Dissertations

Information on reproduction in elasmobranchs is important for the management and conservation of populations. However, the ability to obtain reliable data on elasmobranch reproduction without animal euthanasia is extremely limited. Therefore, there has been a call for nonlethal approaches for identifying reproductive status of elasmobranchs. Although useful, current techniques can be limited with respect to access to equipment and reliability of blood analyses. This study investigates whether a new noninvasive approach, the examination of coelomic fluid (CF), can provide a reliable indicator of reproductive status. CF is fluid that bathes the internal organs of the abdominal cavity, including ovaries, and …

Stress And Behavior In Beef Calves During Weaning, Kathleen E. Tierney

Theses and Dissertations

Animal welfare in commercial livestock operations has been an increasing topic of concern. It is well known that stress can have significant behavioral and physiological repercussions. It is especially important in commercial operations to reduce stress, as animal efficiency is the leading priority. In an effort to reduce stress on animals and improve welfare in livestock operations, several studies have evaluated common husbandry practices. One of the most widely studied practices is weaning, which encapsulates the process of removing the young from the dam. Several studies have evaluated the effects of weaning on beef cattle through a variety of behavioral …

A Case Of Acromegaly, Zhiwei Zhang, Donald Eagerton

South Atlantic Division GME Research Day 2025

No abstract provided.

Fiber Photometry Analysis Of Spontaneous Dopamine Signals: The Z-Scored Data Are Not The Data, Conner W. Wallace, Clare Y. Slinkard, Rachael Shaughnessy, Katherine M. Holleran, Samuel W. Centanni, Christopher C. Lapish, Sara R. Jones

Department of Medicine Faculty Publications

Fluorescent sensors have revolutionized the measurement of molecules in the brain, and the dLight dopamine sensor has been used extensively to examine reward- and cue-evoked dopamine release, but only recently has the field turned its attention to spontaneous release events. Analysis of spontaneous events typically requires evaluation of hundreds of events over minutes to hours, and the most common method of analysis, z-scoring, was not designed for this purpose. Here, we compare the accuracy and reliability of three different analysis methods to identify pharmacologically induced changes in dopamine release and uptake in freely moving C57BL/6J mice. The D1-like receptor antagonist …

Science, Physiology, And Nutrition For The Nonscientist, Judi S. Morrill

Open Educational Resources

A wonderful blend of physiology, nutrition, biochemistry, genetics, biology, evolution, chemistry--what we all need to know as informed citizens. A basic knowledge of the life sciences and how our bodies work--to promote our own good health, especially as we're bombarded with misleading advertisements, soundbites, and the like. DNA fingerprinting, calorie requirements, dietary advice, genetic engineering (including gene editing with CRISPR cas9)--all in an easy-to understand book.

The Role Of Tgf-Beta Superfamily Members In Immune Homeostasis And Disease, Natalie Eva Nieuwenhuizen, Ioannis Eleftherianos, Piotr Jan Kraj, Maria Semitekolou

Biological Sciences Faculty Publications

[Introduction] The Transforming Growth Factor beta (TGF-beta) superfamily, encompassing molecules such as TGFs, activins, Bone Morphogenetic Proteins (BMPs), Growth/Differentiation Factor (GDFs) and Nodals, represents the largest family of growth and differentiation factors, playing crucial roles in developmental and physiological processes across animal species (1). These molecules are integral to tissue homeostasis and cell fate determination. Among them, TGF-beta is particularly noted for its regulatory influence on immune responses and tissue fibrosis (2, 3). Recent research has expanded our understanding of the immune functions of other superfamily members, including activin A and BMPs (4). These molecules signal through receptor complexes composed …

Beyond Strong: A Phenomenology Of Women Executives And The Connection Between Leadership And Fitness, Nozomi Bullock

Antioch University Dissertations & Theses

Senior-level executive leaders’ work in a dynamic, competitive, 24/7 business environment can be physically rigorous, mentally demanding, and emotionally isolating. The same holds true for women pursuing senior-level leadership roles in corporate America. Women who achieve and succeed in such roles remain the minority: Outsiders in elite spaces who are held to higher standards and overly scrutinized. Among senior-level executive women leaders, those committed to a fitness and training regimen including weightlifting constitute an even smaller minority. Senior-level executive roles require stamina, resilience, and courage—qualities that make up the foundation of the mindset of fitness and strength training (Foyster et …

Lactate's Role In Appetite Suppression, Seth Mccarthy

Theses and Dissertations (Comprehensive)

Once considered metabolic waste produced during exercise, lactate is now known to have diverse roles in biology. Muscle-derived lactate can travel through the bloodstream and be taken up by other tissues and i) oxidized, ii) converted to glucose via gluconeogenesis, or iii) bind to its receptor to trigger molecular signaling cascades. Lactate has been described as a “myokine” capable of inter-organ crosstalk and compelling evidence demonstrates lactate’s role as a signalling molecule in pathways related to the regulation of appetite. The purpose of this dissertation was to further elucidate lactate’s role in appetite regulation using series of studies aimed at …

Leucine Catabolism Is Associated With Longevity In Female Eastern Lubber Grasshoppers, Kerri R. Conklin

UNF Graduate Theses and Dissertations

Background & Aims: Dietary isoleucine restriction can improve metabolic health and increase lifespan. It has been hypothesized that shifts in branched-chain amino acid catabolism drives this longevity effect. Impaired branched-chain amino acid catabolism is linked to several age-related diseases. We investigated whether dietary isoleucine restriction increases leucine catabolism and associates with longevity and full reproduction.

Methods: In two experiments, adult female Eastern Lubber grasshoppers (Romalea microptera) were assigned to either a high-quality diet (Balanced ile), or diets with varying isoleucine content (Moderate ile-R and Severe ile-R). In Experiment 1, we used a low-energy (Low Lettuce) group as a positive …

Computational Approaches For Diagnosing Neurological Impairments And Restoring Functional Movement, Serhii Bahdasariants

Graduate Theses, Dissertations, and Problem Reports (ETD)

Movement is the primary means by which the nervous system converts intention into interaction with the environment. Voluntary limb motion enables standing, walking, reaching, and grasping—actions that underpin self‑care, communication, and economic participation. When diseases or injuries disrupt the pathways that couple neural commands to muscle forces—whether through cortical stroke, spinal cord injury, or peripheral nerve damage—mechanical outcomes such as weakness, loss of coordination, and ultimately reduced mobility emerge. These impairments cascade into secondary complications, increased dependence on caregivers, and diminished quality of life. Quantifying how the lesion alters the mechanics of movement is therefore essential for designing targeted interventions …

Neuroendocrine Regulation Of The Luteinizing Hormone Surge And Puberty Onset In Sheep, Max J. Griesgraber

Graduate Theses, Dissertations, and Problem Reports (ETD)

Gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH), and follicle stimulating hormone (FSH) are essential for the reproduction of all mammalian species, including both sheep and humans. GnRH is secreted by hypothalamic neurons into the hypophyseal portal vasculature in a pulsatile manner, which then acts within the anterior pituitary to elicit the release of LH and FSH into wider systemic circulation. In females LH and FSH act on the ovaries to promote estradiol (E2) production, follicular development, and under certain conditions in regard to LH, ovulation. Furthermore, while E2 inhibits GnRH and subsequent LH secretion under most conditions, at high concentrations E2 …

Reversible Degradation Of Peptidoglycan By Lytic Transglycosylases, Aaron Devereaux

Theses and Dissertations (Comprehensive)

Antimicrobial resistance continues to be a burden on the global healthcare system with an estimated cost of billions of dollars and millions of deaths each year. Recently, there has been little to no development on new antibiotics to treat bacterial infection, and any that are developed become resisted to within a few years. Both Gram-positive and Gram-negative bacteria contain a mesh-like layer called peptidoglycan (PG) that surrounds their cells providing strength, cell shape, and protection from their environments. This layer is a polymer of N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) connected via a b-1,4-glycosidic bond, with each strand being cross-linked …